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Journal articles on the topic 'Illumina Mi-Seq'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Urumbil, Sithara K., and M. Anil Kumar. "Diversity Analysis of Endophytic Bacterial Microflora in Emilia sonchifolia (Linn.) DC on Illumina Mi Seq Platforms." Journal of Pure and Applied Microbiology 14, no. 1 (2020): 679–87. http://dx.doi.org/10.22207/jpam.14.1.70.

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Haenel, Quiterie, Oleksandr Holovachov, Ulf Jondelius, Per Sundberg, and Sarah Bourlat. "NGS-based biodiversity and community structure analysis of meiofaunal eukaryotes in shell sand from Hållö island, Smögen, and soft mud from Gullmarn Fjord, Sweden." Biodiversity Data Journal 5 (June 8, 2017): e12731. https://doi.org/10.3897/BDJ.5.e12731.

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Aim: The aim of this study was to assess the biodiversity and community structure of Swedish meiofaunal eukaryotes using metabarcoding. To validate the reliability of the metabarcoding approach, we compare the taxonomic resolution obtained using the mitochondrial cytochrome oxidase 1 (COI) 'mini-barcode' and nuclear 18S small ribosomal subunit (18S) V1-V2 region, with traditional morphology-based identification of Xenacoelomorpha and Nematoda. Location: 30 samples were analysed from two ecologically distinct locations along the west coast of Sweden. 18 replicate samples of coarse shell sand we

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Atherton, Sarah, and Ulf Jondelius. "Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding." Biodiversity Data Journal 8 (April 27, 2020): e51813. https://doi.org/10.3897/BDJ.8.e51813.

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The meiofauna is an important part of the marine ecosystem, but its composition and distribution patterns are relatively unexplored. Here we assessed the biodiversity and community structure of meiofauna from five locations on the Swedish western and southern coasts using a high-throughput DNA sequencing (metabarcoding) approach. The mitochondrial cytochrome oxidase 1 (COI) mini-barcode and nuclear 18S small ribosomal subunit (18S) V1-V2 region were amplified and sequenced using Illumina MiSeq technology. Our analyses revealed a higher number of species than previously found in other areas: th

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Zhang, Fengling, Xingjia Xiang, Yuanqiu Dong, Shaofei Yan, Yunwei Song, and Lizhi Zhou. "Significant Differences in the Gut Bacterial Communities of Hooded Crane (Grus monacha) in Different Seasons at a Stopover Site on the Flyway." Animals 10, no. 4 (2020): 701. http://dx.doi.org/10.3390/ani10040701.

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Intestinal bacterial communities form an integral component of the organism. Many factors influence gut bacterial community composition and diversity, including diet, environment and seasonality. During seasonal migration, birds use many habitats and food resources, which may influence their intestinal bacterial community structure. Hooded crane (Grus monacha) is a migrant waterbird that traverses long distances and occupies varied habitats. In this study, we investigated the diversity and differences in intestinal bacterial communities of hooded cranes over the migratory seasons. Fecal sample

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Nadifah, Fitri, Wayan Tunas Artama, Budi Setiadi Daryono, and Endah Retnaningrum. "Characterization of the urogenital microbiome in patients with urinary tract infections." Indonesian Journal of Biotechnology 27, no. 3 (2022): 142. http://dx.doi.org/10.22146/ijbiotech.69212.

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Standard microbiological culture techniques can only identify a fraction of the urogenital microbiome. Meanwhile, identifying and characterizing infectious microorganisms are very important for the success of diagnosis and treatments, especially for Urinary Tract Infection (UTI) patients. This study aimed to characterize the urogenital microbiome of UTI patients using 16S rRNA gene sequencing. We sequenced two pooled DNA samples from voided urine of UTI patients (21 females and 13 males). To determine the structure and composition of taxa in the samples, 16S rRNA gene sequencing was performed

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Kushkevych, Ivan, Monika Vítězová, Tomáš Vítěz, and Milan Bartoš. "Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms." Open Life Sciences 12, no. 1 (2017): 82–91. http://dx.doi.org/10.1515/biol-2017-0009.

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AbstractThe production of high-quality methane depends on many factors, including temperature, pH, substrate, composition and relationship of the microorganisms. The qualitative and quantitative composition of methanogenic and sulfate-reducing microorganisms and their relationship in the experimental bioreactors has never been studied. The aim of this research was to characterize, for the first time, the diversity of the methanogenic microorganisms and sulfate-reducing bacteria, and study their relationship and biogas production in experimental bioreactors. Amplification of 16S rRNA gene fragm

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Chitwood, J. L., V. R. Burruel, S. A. Meyers, and P. J. Ross. "131 RNA-Seq TRANSCRIPTOME PROFILING OF INDIVIDUAL RHESUS MACAQUE OOCYTES AND PRE-IMPLANTATION EMBRYOS." Reproduction, Fertility and Development 26, no. 1 (2014): 179. http://dx.doi.org/10.1071/rdv26n1ab131.

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The objective of this study was to perform whole transcriptome sequencing (RNA-Seq) on individual rhesus macaque oocytes and intracytoplasmic sperm injection-derived embryos. Nine developmental stages were assayed (n = 2–3 per stage): germinal vesicle (GV), meiosis I (MI), and meiosis II (MII) oocytes and pronuclear (PN), 2-cell (2C), 4-cell (4C), 8-cell (8C), morula (MO), and blastocyst (BL) stage embryos. Rhesus females were superovulated with hrFSH and oocytes collected by follicular aspiration of ovaries recovered at necropsy 35 h after hCG treatment. Based on GV and polar body (PB) presen

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Jones, James M. C., Elizabeth A. Webb, Michael D. J. Lynch, Trevor C. Charles, Pedro M. Antunes, and Frédérique C. Guinel. "Does a carbonatite deposit influence its surrounding ecosystem?" FACETS 4, no. 1 (2019): 389–406. http://dx.doi.org/10.1139/facets-2018-0029.

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Carbonatites are unusual alkaline rocks with diverse compositions. Although previous work has characterized the effects these rocks have on soils and plants, little is known about their impacts on local ecosystems. Using a deposit within the Great Lakes–St. Lawrence forest in northern Ontario, Canada, we investigated the effect of a carbonatite on soil chemistry and on the structure of plant and soil microbial communities. This was done using a vegetation survey conducted above and around the deposit, with corresponding soil samples collected for determining soil nutrient composition and for a

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Baptista Neto, José Antônio, Christine Gaylarde, Iwona Beech, Bernard J. Smith, and John J. McAlister. "Degradação de gnaisse e granito em fachadas de edifícios históricos no centro do Rio de Janeiro." Sistemas & Gestão 15, no. 1 (2020): 80–90. http://dx.doi.org/10.20985/1980-5160.2020.v15n1.1621.

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A maioria do patrimônio cultural construído na cidade do Rio de Janeiro, Brasil, é feito de gnaisse ou granito, e a exposição das fachadas ao ambiente poluído leva a uma forte degradação. Para entender estes processos meteorológicos, foram estudados cinco edifícios históricos na cidade. Estes exibiram manchas de ferro, desintegração granular, bolhas, fraturas incipientes e escamação de contornos e desenvolvimento de crosta negra. As amostras coletadas nestes edifícios foram examinadas na tentativa de compreender os mecanismos da meteorologia de superfície. Amostras de rochas foram coletadas em

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Pellegrini, Marika, Daniela M. Spera, Claudia Ercole, and Maddalena del Gallo. "Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plant Growth and Development and Soil Fertility Status and Microbial Community." Proceedings 66, no. 1 (2021): 20. http://dx.doi.org/10.3390/proceedings2020066020.

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The present work was aimed at investigating the effects of a four strains consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-S

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Pellegrini, Marika, Daniela M. Spera, Claudia Ercole, and Maddalena Del Gallo. "Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plants, Soil, and the Autochthonous Microbial Community." Microorganisms 9, no. 3 (2021): 639. http://dx.doi.org/10.3390/microorganisms9030639.

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The present work was aimed at investigating the effects of a four bacterial strain consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on Allium cepa L. and on soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated and Control seeds (treated with autoclaved inoculum) were sown in an open-field and followed until harvest. Plant growth development parameters, as well as soil physico–chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq

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Pellegrini, Marika, Daniela M. Spera, Claudia Ercole, and Maddalena del Gallo. "Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plant Growth and Development and Soil Fertility Status and Microbial Community." Proceedings 66, no. 1 (2021): 20. http://dx.doi.org/10.3390/proceedings2020066020.

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The present work was aimed at investigating the effects of a four strains consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-S

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Shchubelka, K., Walter W. Wolfsberger, O. Oleksyk, and Et All. "Genomics of Type 1 Diabetes in Ukraine Initiative." INTERNATIONAL JOURNAL OF ENDOCRINOLOGY (Ukraine) 21, no. 4 (2025): 343–50. https://doi.org/10.22141/2224-0721.21.4.2025.1557.

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Type 1 diabetes (T1D) is a complex autoimmune disorder with a strong genetic component. While genome-wide association studies have identified over 90 loci associated with T1D, the contribution of rare, coding, and population-specific variants remains poorly explored. A new international collaborative effort, based in Ukraine in partnership with Uzhhorod National University and coordinated with Oakland University, was launched to enrol 20,000 individuals (10,000 clinically confirmed T1D cases and 10,000 ethnically/geographically matched controls) from Ukraine and neighbouring Poland, generate h

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Z, Mirakbarova, and Turdikulova S. H. "Targeted Hot Spot Sequencing of Uzbek Lung Cancer Patients." Biomedical and Pharmacology Journal 14, no. 1 (2021): 385–90. http://dx.doi.org/10.13005/bpj/2138.

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Objectives: Carcinoma of lung is a widespread multifactorial disease with a poor prognosis. Uzbek lung cancer patients screening for prevalent mutations, associated with cancer development by targeted next generation sequencing was the main objective of current research. Materials and methods: Preparation of sequencing libraries was conducted using the Illumina Cancer Hot spot Panel v2 covering mutational hot spot regions of 50 cancer genes, from DNA extracted from formalin-fixed paraffin-embedded tumor samples of 10 patients. Sequencing was performed with the Mi Seq Next Generation Sequencing

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Rabee, Alaa Emara, Amr A. Sayed Alahl, Mebarek Lamara, and Suzanne L. Ishaq. "Fibrolytic rumen bacteria of camel and sheep and their applications in the bioconversion of barley straw to soluble sugars for biofuel production." PLOS ONE 17, no. 1 (2022): e0262304. http://dx.doi.org/10.1371/journal.pone.0262304.

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Lignocellulosic biomass such as barley straw is a renewable and sustainable alternative to traditional feeds and could be used as bioenergy sources; however, low hydrolysis rate reduces the fermentation efficiency. Understanding the degradation and colonization of barley straw by rumen bacteria is the key step to improve the utilization of barley straw in animal feeding or biofuel production. This study evaluated the hydrolysis of barley straw as a result of the inoculation by rumen fluid of camel and sheep. Ground barley straw was incubated anaerobically with rumen inocula from three fistulat

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Babiker, Hani M., Alex John Liu, Alejandro Recio Boiles, et al. "Correlative analysis of circulating tumor (ct) DNA and tumor mutational analysis (TMA) in patients with advanced pancreatobilliary malignancies (PBM)." Journal of Clinical Oncology 36, no. 4_suppl (2018): 235. http://dx.doi.org/10.1200/jco.2018.36.4_suppl.235.

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235 Background: Tumor archival tissue (TT) MA of PBM is challenging due to insufficient specimen acquired from fine needle aspirations. This is a barrier to analyzing specimens for targeted therapy (clinical trial enrollment). The correlation of cell free ctDNA and TMA has not been studied extensively. We hypothesize that somatic TMA correlates with ctDNA and will be a surrogate for enrollment to targeted trials. Methods: We retrospectively identified PBM patients evaluated in the Phase I Program and analyzed ctDNA and TT. ctDNA was extracted from plasma, genomic alterations were analyzed by p

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Urbini, Milena, Annalisa Astolfi, Valentina Indio, et al. "Gene fusions evidence in a KIT/PDGFRA wild-type GIST without mutations in SDH units identified by a whole transcriptome study." Journal of Clinical Oncology 31, no. 15_suppl (2013): e21523-e21523. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e21523.

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e21523 Background: A subset of KIT/PDGFRA wild-type GIST (WT) harbour mutations in SDH units. In the majority of the remaining cases of WT GIST no other molecular events are identified.We performed a RNA-seq in a WT GIST without mutations in SDH genes using next generation approach to discover molecular events in this GIST population. Methods: In 2003, a 63-year old woman underwent surgery for an ileal GIST (size 6 cm, MI 6/50HPF).After 6 years, she developed a recurrence with a single hepatic lesion. The KIT and PDGFRA analysis of the lesion did not show mutations. Therefore, she did not rece

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Sakda, Patthanan, Xingjia Xiang, Zhongqiao Song, Yuannuo Wu, and Lizhi Zhou. "Impact of Season on Intestinal Bacterial Communities and Pathogenic Diversity in Two Captive Duck Species." Animals 13, no. 24 (2023): 3879. http://dx.doi.org/10.3390/ani13243879.

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Vertebrates and their gut bacteria interact in complex and mutually beneficial ways. The intestinal microbial composition is influenced by several external influences. In addition to food, the abiotic elements of the environment, such as temperature, humidity, and seasonal fluctuation are also important determinants. Fecal samples were collected from two captive duck species, Baikal teal (Sibirionetta formosa) and common teal (Anas crecca) across four seasons (summer, autumn, winter, and spring). These ducks were consistently fed the same diet throughout the entire experiment. High throughput

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Mahtab, Nazia, Lizhi Zhou, Fengling Zhang, and Wei Wang. "Seasonal Variations in the Gut Fungal Communities of Hooded Crane (Grus monacha) at Wintering and Stopover Sites in China." Animals 11, no. 4 (2021): 941. http://dx.doi.org/10.3390/ani11040941.

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The “gut fungal microbiome” maintains the immune system, homeostasis, and various physiological functions of an organism. Different factors shape and affect gut fungal diversity and community composition, such as environment, habitat type, food resources, and seasons during migration. Wild birds amid migration are exposed to different habitats with different environments, available food resources, and seasons, which may substantially impact their gut fungal community composition and diversity. The hooded crane (Grus monacha) is a known migratory bird that migrates over long distances and is ex

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Yap, C. F., N. Nair, S. Verstappen, K. Hyrich, A. Barton, and D. Plant. "POS0351 MULTI-OMICS ANALYSIS IDENTIFIES DIFFERENTIAL LIPID REGULATION AS A POTENTIAL MECHANISM UNDERPINNING METHOTREXATE RESPONSE IN RHEUMATOID ARTHRITIS." Annals of the Rheumatic Diseases 82, Suppl 1 (2023): 425.2–425. http://dx.doi.org/10.1136/annrheumdis-2023-eular.744.

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BackgroundRheumatoid arthritis (RA) is a chronic autoimmune disease that causes inflammation and joint damage, leading to disability and reduced quality of life. Methotrexate (MTX) is a widely used disease-modifying anti-rheumatic drug that is initially prescribed to reduce inflammation and slowing disease progression in RA; however, approximately 40% of treated patients do not experience a satisfactory response to this drug. Identifying reliable surrogate biomarkers of MTX response would facilitate precision medicine strategies and improve patient outcomes.ObjectivesTo identify blood-based tr

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Parker, Helen, Amatta Mirandari, Carolina Jaramillo-Oquendo, et al. "The Proliferative History Index, Epicmit, Derived from Genome-Wide Epigenomic Profiling, Is a Key Driver of Clinical Survival in Splenic Marginal Zone Lymphoma." Blood 142, Supplement 1 (2023): 4370. http://dx.doi.org/10.1182/blood-2023-174481.

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The epiCMIT (epigenetically-determined Cumulative MIToses) mitotic clock (Durran-Ferrer, 2020) tracks DNA hypo- and hyper-methylation changes in heterochromatin and H3K27me3-containing chromatin, respectively, providing a comprehensive record of B cell mitotic history (pre- and post-malignant transformation). Higher epiCMIT levels correlate with poor survival in CLL and MCL, suggesting that a greater mitotic history predicts future proliferative capacity. However, no studies have investigated epiCMIT in splenic marginal zone lymphoma (SMZL). We studied 142 SMZL cases, a rare lymphoid malignanc

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Vey, Norbert, Marie Anne Hospital, Aude Collignon, et al. "Cegal Protocol : Evaluation of the Feasibility of a Chemogenomic Approach to Identify Personalized Therapy for Relapse or Refractory AML Patients." Blood 132, Supplement 1 (2018): 1401. http://dx.doi.org/10.1182/blood-2018-99-119263.

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Abstract Introduction : Significant improvements of leukemia free survival have been obtained over the last 30 years in AML. This progress has resulted from optimization of the same chemotherapy-based "one-size-fits-all" approach. However, even though the majority of patients eligible for intensive chemotherapy achieve complete remission (60% to 80%), the majority still relapse and ultimately die (5-year overall survival being around 20%). Recent genomic studies have revealed the heterogeneity of AML at the molecular level, stressing the need for personalized therapeutic approaches. The fundam

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Bonadies, Nicolas, Vladislav Grigorjev, Naomi Porret, et al. "Multiomics Profiling Reveals Higher RNA Isoform Diversity and Potential Oncospecific Protein Isoform Targets in HSPCs of MDS and MDS/MPN Patients." Blood 142, Supplement 1 (2023): 4592. http://dx.doi.org/10.1182/blood-2023-178092.

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Background: Myelodysplastic Syndromes/Neoplasms (MDS) are characterized by somatic driver mutations in hematopoietic stem/progenitor cells (HSPCs) leading to hypermethylation, disruption of normal hematopoiesis and progression to acute myeloid leukemia ( Cazzola M, N Engl J Med 2020). With exception of a minority of fit patients, who are candidates for treatment with the potentially curative allogenic hematopoietic stem cell transplantation (allo HSCT), most elderly patients remain incurable and receive palliative treatment with hypomethylating agents. Due to the high phenotypic overlap betwee

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Gallage, Kavishka S., Alexander Van Nynatten, Nathan K. Lujan, Nathan R. Lovejoy, and Nicholas E. Mandrak. "Identifying early life stages of Great Lakes fishes using a metabarcoding approach." Canadian Journal of Fisheries and Aquatic Sciences, September 6, 2023. http://dx.doi.org/10.1139/cjfas-2023-0061.

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Detection of early life stages of fishes is important for understanding life history patterns and critical spawning habitats. When feasible, identifying early life stages of fishes using morphology requires taxonomic expertise and can be challenging, time consuming, and imprecise. In this study, we used DNA metabarcoding to identify egg and larval batch samples from two sites in the species-rich East Sydenham River, Ontario, Canada. We used a two-step PCR metabarcoding approach to amplify a highly variable region of the mitochondrial COI gene from 1075 mixed species batch samples. Amplicon lib

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Ángeles-Argáiz, Rodolfo Enrique, Luis Fernando Lozano Aguirre-Beltrán, Diana Hernández-Oaxaca, et al. "Assembly collapsing versus heterozygosity oversizing: detection of homokaryotic and heterokaryotic Laccaria trichodermophora strains by hybrid genome assembly." Microbial Genomics 10, no. 3 (2024). http://dx.doi.org/10.1099/mgen.0.001218.

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Genome assembly and annotation using short-paired reads is challenging for eukaryotic organisms due to their large size, variable ploidy and large number of repetitive elements. However, the use of single-molecule long reads improves assembly quality (completeness and contiguity), but haplotype duplications still pose assembly challenges. To address the effect of read length on genome assembly quality, gene prediction and annotation, we compared genome assemblers and sequencing technologies with four strains of the ectomycorrhizal fungus Laccaria trichodermophora. By analysing the predicted re

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Srivastava, Subodh K., Kurt Zeller, James H. Sobieraj, and Mark K. Nakhla. "Genome resources of four distinct pathogenic races within Fusarium oxysporum f. sp. vasinfectum that cause vascular wilt disease of cotton." Phytopathology®, August 31, 2020. http://dx.doi.org/10.1094/phyto-07-20-0298-a.

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Whole Genome Sequence (WGS) based identifications are being increasingly used by regulatory and public health agencies to facilitate the detection, investigation, and control of pathogens and pests. Fusarium oxysporum f. sp. vasinfectum (FOV) is a significant vascular wilt pathogen of cultivated cotton, and consists of several pathogenic races that are not each other’s closest phylogenetic relatives. We have developed WGS assemblies for isolates of race 1 (FOV1), race 4 (FOV4), race 5 (FOV5), and race 8 (FOV8) using a combination of Nanopore (MinION) and Illumina sequencing technology (Mi-Seq)

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Han, Zhiguang, Cong Wang, Binghai Lei, et al. "A limited overlap of interactions between the bacterial community of water and sediment in wetland ecosystem of the Yellow River floodplain." Frontiers in Microbiology 14 (June 22, 2023). http://dx.doi.org/10.3389/fmicb.2023.1193940.

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IntroductionAquatic ecosystems in floodplains provide homes for a variety of active bacterial populations. However, the coexistence pattern of bacterial communities of water and sediment in these ecosystems is unclear.MethodsIn the present study, Illumina Mi-Seq sequencing were to assess bacteria's co-occurrence patterns in the water and sediment of different time dynamics and plant communities of the Yellow River floodplain ecosystem.Results and discussionThe results showed that compared to water, the α-diversity of the bacterial community was way greater in sediment. The bacterial community

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Allam, Sahar A., Eman Mahrous, Sahar T. M. Tolba, Samy M. Mohamed, Mohamed T. Ragab, and Rania I. Mohamed. "Detection of Mycobacteria in Arabian camels and antimycobacterial potential of Moringa oleifera." Scientific Reports 15, no. 1 (2025). https://doi.org/10.1038/s41598-025-92402-0.

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Abstract The World Health Organization gave great attention to Mycobacterium tuberculosis, especially its zoonotic impact. Dromedary camels in Arabian countries are of great importance, as well as awareness of production and health. Little was known about the occurrence of M. tuberculosis among Arabian camels. Out of 88 samples were collected from necropsied male camels aged 5–6.5 years after the slaughter process resident in Cairo abattoir. Isolation of Mycobacteria was achieved on Middle Brook 7H10 agar with special supplements, and then the suspected colonies were assessed by their specific

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Pottker, Emanuele Serro, Bruna Webber, Samuel Cibulski, et al. "Genomics and Phenotypical Characterization of Two New Lytic Bacteriophages for Biocontrol of Salmonella enterica." Microbiology Research Journal International, December 31, 2020, 93–105. http://dx.doi.org/10.9734/mrji/2020/v30i1030277.

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Aims: To perform the isolation, characterization and sequencing of the bacteriophages. To demonstrate that the bacteriophages can be used for biocontrol of different Salmonella enterica serovars. Study Design: This study was an experimental study. Place and Duration of Study: Bacteriology and Mycology Laboratory in the Veterinary Hospital at the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo (FAMV/UPF), Biotechnology Center (CBiotec) of the Federal University of Paraíba (UFPB), Center for Microscopy and Microanalysis at the Faculty of Veterinary of the

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Baloh, Philippe, Nora Els, Robert O. David, et al. "Assessment of Artificial and Natural Transport Mechanisms of Ice Nucleating Particles in an Alpine Ski Resort in Obergurgl, Austria." October 4, 2019. https://doi.org/10.3389/fmicb.2019.02278.

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Artificial snow production is a crucial part of modern skiing resorts in Austria and globally, and will develop even more so with changing precipitation patterns and a warming climate trend. Producing artificial snow requires major investments in energy, water, infrastructure and manpower for skiing resorts. In addition to appropriate meteorological conditions, the efficiency of artificial snow production depends on heterogeneous ice-nucleation, which can occur at temperatures as high as −2°C when induced by specific bacterial ice nucleating particles (INPs). We aimed to investigate

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Yu, Yanyan, Hao Liu, Lanlan Zhang, et al. "Distinct response patterns of plants and soil microorganisms to agronomic practices and seasonal variation in a floodplain ecosystem." Frontiers in Microbiology 14 (January 26, 2023). http://dx.doi.org/10.3389/fmicb.2023.1094750.

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IntroductionClimate change and anthropogenic activities are the greatest threats to floodplain ecosystems. A growing body of literature shows that floodplain ecosystems have experienced increased chemical fertilizer and pesticide loads, which will disturb the above and belowground ecosystems. However, we lack knowledge regarding the effects of such human activities on the vegetation and soil microbiomes in these ecosystems.MethodsIn the present study, plant functional traits and Illumina Mi-Seq sequencing were to assess the impact of nitrogen fertilizer and glyphosate addition on the structure

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Ioele, D., R. Paolillo, S. D'apice, et al. "Partial deletion of the Akap1 gene promotes abnormalities in gut barrier function, gut microbiota composition and cardiac function during cardiovascular aging in mice." European Heart Journal 45, Supplement_1 (2024). http://dx.doi.org/10.1093/eurheartj/ehae666.3681.

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Abstract Background Cardiovascular aging is characterized by mitochondrial dysfunction, changes in intestinal permeability and microbiota composition. Mitochondria-targeted A-kinase anchoring proteins (mitoAKAPs) are a group of structurally diverse proteins binding the regulatory subunits of protein kinase A (PKA), and targeting PKA at discrete intracellular locations. We analyzed the effects of global partial genetic deletion of Akap1 on intestinal barrier permeability, microbiota composition, inflammation, and cardiac function during aging in mice. Purpose The proposal of this study was to d

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Paolillo, R., N. Boccella, L. Coretti, et al. "Aging-associated cardiac dysfunction and leaky gut in heterozygous Akap1 knockout mice." European Heart Journal 41, Supplement_2 (2020). http://dx.doi.org/10.1093/ehjci/ehaa946.3605.

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Abstract Background Mitochondrial A-kinase anchoring proteins (mitoAKAPs) encoded by the Akap1 gene are crucially involved in multiple cellular processes, including cardiomyocyte survival and function. There is a correlation between ROS production, intestinal permeability and the composition of the intestinal microbiota, especially during aging. Whether Akap1 deletion affects microbiota composition, intestinal function during senescence is currently unknown. Purpose The purpose of the study was to shed light into the complex interplay between gut permeability and microbiota composition, cardia

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Zhang, Xiaoling, Yoshiyuki Wakabayashi, Shih-Jen Hwang, et al. "Abstract 15476: Genome-wide Transcriptome Study for Myocardial Infarction and Coronary Artery Calcification Using RNA-Seq." Circulation 132, suppl_3 (2015). http://dx.doi.org/10.1161/circ.132.suppl_3.15476.

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Introduction: Coronary artery calcification (CAC) is a noninvasive measure of coronary atherosclerosis, which underlies most cases of myocardial infarction (MI). Genome-wide association (GWA) studies have identified common genetic variants associated with MI and CAC. However, the mechanisms underlying MI and CAC remain unclear. We sought to identify gene expression signatures of early MI and high CAC, and further investigate the underlying mechanisms between clinical MI and subclinical atherosclerosis in the Framingham Heart Study (FHS). Methods: We conducted a paired-end RNA sequencing on who

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D'Apice, S., R. Paolillo, N. Boccella, et al. "Partial loss of Akap1 promotes cardiac dysfunction, gut barrier dysfunction and alterations of gut microbiota composition during aging." European Heart Journal 42, Supplement_1 (2021). http://dx.doi.org/10.1093/eurheartj/ehab724.3302.

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Abstract Background Mitochondrial A-kinase anchoring proteins (mitoAKAP) encoded by the Akap1 gene are crucially involved in multiple cellular processes, including cardiomyocyte survival and function. Whether mitoAKAP levels play a role in aging and gut microbiota composition is currently unknown. Purpose The aim of this study was to highlight the complex interplay between cardiac dysfunction, gut barrier integrity, gut microbiota composition and aging in young (6-month-old, 6m) and old (24-month-old, 24m) wild type (wt) and Akap1 heterozygous mice (Akap1+/−). Methods Cardiac function was anal

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Paolillo, R., N. Boccella, L. Coretti, et al. "Transverse aortic constriction impairs intestinal barrier integrity, promotes inflammation and alterations in gut microbiota composition." European Heart Journal 41, Supplement_2 (2020). http://dx.doi.org/10.1093/ehjci/ehaa946.3667.

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Abstract Background Microbiota composition plays an important role in the pathophysiology of heart failure (HF). Reduced cardiac output can disrupt intestinal barrier function and promote systemic inflammation through bacterial translocation. Several gut and cardiac pathological conditions are reciprocally linked at multiple levels and through different, still largely undefined mechanisms. Purpose We analyzed the effects of Transverse aortic constriction (TAC) on aortic pressures, gut barrier integrity, systemic inflammation and gut microbiota composition. Methods TAC was induced in C57BL6 mic

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D´apice, Stefania, Roberta Paolillo, Lorena Coretti, et al. "523 MITOAKAP PLAY A ROLE IN THE PROGRESSION TOWARDS HEART FAILURE MODULATING GUT INTEGRITY AND COMPOSITION DURING AGING." European Heart Journal Supplements 24, Supplement_K (2022). http://dx.doi.org/10.1093/eurheartjsupp/suac121.414.

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Abstract Background Mitochondrial A-kinase anchoring proteins (mitoAKAP) encoded by the Akap1 gene promote Protein Kinase A mitochondrial targeting, regulating mitochondrial structure and function, reactive oxygen species production and cardiomyocyte survival. Whether mitoAKAP levels play a role in cardiac aging, gut barrier integrity and gut microbiota composition is currently unknown. Purpose The aim of this study was to highlight the complex interplay between cardiac dysfunction, gut barrier integrity, gut microbiota composition and aging in young (6-month-old, 6m) and old (24-month-old, 24

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D’Apice, Stefania, Roberta Paolillo, Lorena Coretti, Giovanni Esposito, Francesca Lembo, and Cinzia Perrino. "186 Partial loss of AKAP1 promotes cardiac dysfunction, gut barrier abnormalities, and alteration of gut microbiota composition during ageing." European Heart Journal Supplements 23, Supplement_G (2021). http://dx.doi.org/10.1093/eurheartj/suab139.010.

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Abstract Aims Mitochondrial A-kinase anchoring proteins (mitoAKAP) encoded by the Akap1 gene promote Protein Kinase A mitochondrial targeting, regulating mitochondrial structure and function, reactive oxygen species production, and cardiomyocyte survival. Whether mitoAKAP levels play a role in cardiac ageing, gut barrier integrity and gut microbiota composition is currently unknown. The aim of this study was to highlight the complex interplay between cardiac dysfunction, gut barrier integrity, gut microbiota composition and ageing in young (6-month-old, 6 m) and old (24-month-old, 24 m) wild t

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