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Dissertations / Theses on the topic 'Immune-modulatory effects'
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Vos, Arjan Paul. "Preclinical studies on the immune-modulatory effects of dietary oligosaccharides." [Maastricht] : Maastricht : Maastricht University ; University Library, Universiteit Maastricht [host], 2008. http://arno.unimaas.nl/show.cgi?fid=10681.
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Bundscherer, Lena Christina [Verfasser]. "Immune-modulatory effects of non-thermal plasma / Lena Christina Bundscherer." Greifswald : Universitätsbibliothek Greifswald, 2014. http://d-nb.info/1050266897/34.
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Klaesson, Sven. "Immune modulatory effects of intravenous immunoglobulin in vitro and after allogeneic bone marrow transplantation /." Stockholm, 1997. http://diss.kib.ki.se/1997/91-628-2636-0.
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Helal, Racha [Verfasser]. "Biopharmaceutical investigations of the effects of immune-modulatory plant extracts and phytochemicals on lysozyme expression in human cells / Racha Helal." Berlin : Freie Universität Berlin, 2011. http://d-nb.info/1025490010/34.
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Mohan, C. V. "Modulatory effects of cadmium and copper on the susceptibility and immune response of common carp, Cyprinus carpio (L) to selected pathogens." Thesis, University of Stirling, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280056.
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Fernandes, Antonio Celestino. "Investigation of the immune-modulatory effects of erythromycin." Thesis, 1986. https://hdl.handle.net/10539/25754.
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A Dissertation submitted to the Faculty of Medicine, University of the Witwatersrand, Johannesburg, for the Degree of Master of Science (MED).
JOHANNESBURG, 1986The Literature Review covers the immunosuppressive and immunopotentiating properties of antibiotics on the immune system and the effects these could have on the resolution of an infection. The possible pathogenic mechanisms of C. albicans are also reviewed in this section. The experimental section shows that pre-treatment of mice with erythromycin increases the mean survival time following intraperitoneal inoculation of C. albicans. It was shown that erythromycin enhanced lymphocyte transformation and PMNL migration in both in-vivo and in-vitro situations. These enhanced immunological components probably caused improved survival times in the aforementioned animal experiments. To investigate the effects of oral administration of erythromycin on in-vivo PMNL migration in adult volunteers a new quantitative test which could only be applied to humans was developed and is described in detail. Using this method preliminary data were obtained which show that erythromycin increases PMNL migration in-vivo.
IT2018
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Shih, Song-Shan, and 施松杉. "Immune modulatory effects by acupmoxa on cecal ligation and puncture induced septic rats." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/05059083129673110795.
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碩士國立陽明大學
傳統醫藥學研究所
87
Abdominal infection raises severe septic cascades, and usually results in multiple organs failure (MOF). The balance of pro- and anti-inflammatory cytokines is related to disease severity and host survival. Prevention and early treatment remain the management strategy for such abdominal sepsis. Acupuncture and moxibustion (acupmoxa) is well known to control infectious diseases. However, the exact mechanism remains unclear. Our aim is to test the hypothesis that somatic stimulation by electro-acupuncture (EA) or by moxibustion-like local heat (LH) is the better effective management to improve the survival rates and clinical outcomes of septic animals. Abdominal sepsis was induced on female Spraque-Dawley rats (200-300g) by using cecal ligation and puncture (CLP) method. Somatic stimulation by EA or moxibustion-like LH on right upper quadrant acupoints Qimen (Liv-14) and Riyue (GB-24) were used twice a day at 9:00 a.m. and 5:00 p.m. for 5 days after the onset of CLP-induced sepsis. Animals were randomly allocated into four groups. They were sham (negative control), CLP-EA, CLP-LH, and CLP-alone (positive control) group. At definite time points of 0, 8, 24, 72, and 120 hours following CLP, parameters of infection such as hematocrit, SGPT, white blood cells count, serum nitrite level, serum interleukin-1b level and interleukin-10 level, as well as hepatic iNOS gene expression were measured. Finally, the survival rates of the stratification groups were analyzed by Kaplan & Meier''s method. The cumulative survival rates at 24 hr and 48 hr in sham group (n=13), CLP-EA group (n=23), CLP-LH group (n=28) and CLP-alone group (n=25) were 100.0% and 100.0%, 69.6% and 60.9%, 25.0% and 14.3%%, and 48.0% and 44.0%, respectively (p<0.001). The CLP-EA group also showed a better outcome, interms of parameters of infection, on the control of CLP-induced sepsis. The hepatic iNOS gene expression was down-regulated by CLP-EA or CLP-LH manipulation. We conclude that early electro-acupuncture (EA) can improve the clinical outcomes on CLP-induced abdominal sepsis, and provides an easily applicable alternative for those patients with abdominal sepsis.
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Kriegeskorte, Anja Kerstin [Verfasser]. "Analysis of immune-modulatory effects of NKG2D ligands on T cells / Anja Kerstin Kriegeskorte." 2007. http://d-nb.info/985919639/34.
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Chen, Yi-Lien, and 陳逸璉. "The immuno-modulatory effects of polysaccharides from Antrodia camphorata on immune effector cells and OVA-induced asthmatic mice." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/50814301482063018934.
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碩士臺北醫學大學
醫學科學研究所
96
Antrodia camphorata (syn. Taiwanofungus camphorata) is a special medicinal mushroom in Taiwan. Previous studies showed that polysaccharides isolated from Antrodia camphorata (AC-PS) had the anti-inflammation and anti-tumor abilities. However, the modulatory effects of AC-PS in antigen presenting cell (APC) and T cell are unclear. In this study, we first examined the immune-modulatory effects of the purified polysaccharides isolated from Antrodia camphorata (GF-2) on dendritic cells (DCs) and T cells. Our data showed that GF-2 enhanced the production of IL-10 and IL-12 from DCs challenged with LPS. GF-2 also increased the expression of CD80 and CD86 on DCs. However, GF-2 combined with LPS reduced the expression of MHC classⅡ. In addition, GF-2-treated DCs markedly drive T cells to develop into IL-10-secreting T cells. These results indicated that GF-2 promoted the production of IL-10 to modulate immune response in vitro. In vivo, mice were immunized with OVA plus GF-2 to investigate the modulatory effects of GF-2. Our data showed that GF-2 significantly inhibited the production of OVA-specfic IgE and IgG1. GF-2 not only decreased the release of IL-5 but also increased the production of IL-10 and IFN-γ in splenocytes. Considering these results, we suggested that GF-2 could attenuate IL-5 by enhancing the production of IL-10 and IFN-γ. Furthermore, we evaluated the preventive effect of GF-2 in asthmatic animal model. The results showed that GF-2 decreased airway hyperresponsiveness (AHR), eosinophilia and Th2 cytokines secretion. In conclusion, we suggested that GF-2 could modulate the response of asthma and we hope GF-2 might be used as novel therapeutic reagent for asthma treatment.
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Lee, Wan-Yun, and 李宛芸. "The modulatory effects of zerumbone on the immune effector cells and airway inflammation in a murine model of asthma." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/02295449951983433042.
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Lin, Meng-Wei, and 林孟葦. "he modulatory effects of Oridonin on the immune effector cells and airway inflammation in a murine model of asthma." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/925bq6.
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碩士臺北醫學大學
醫學科學研究所
102
Allergic asthma is a chronic inflammatory lung disease of the airway, characterized by airway eosinophil accumulation, goblet cell hyperplasia with mucus hypersecretion, and hyper-responsiveness in response to inhaled allergens. The inflammatory process in allergic asthma is dominated by type 2 T helper (Th2) cells that produce interleukin-5 (IL-5), IL-4 and IL-13, which activate eosinophil and induce immunoglobulin E(IgE) production by B cells.. Oridonin, an ent-kaurane diterpenoid compound (C20H28O6), is isolated from the traditional Chinese herb Rabdosia rubescens. Oridonin has shown anti-tumor, anti-bacterial, and anti-inflammatory properties. Thus, we want to investigate the anti-allergic effects and mechanisms of Oridonin on immune effector cells and ovalbumin(OVA)-induced asthmatic mice. Our data showed that Oridonin inhibited the production of IL-12, IL-10 and IL-6 in LPS-stimulated DCs.However,Oridonin treatment didn’t effect the expression of MHC class II、CD40、CD80、CD86、PD-L1 and CD54 molercules in LPS-stimulated DCs. By mix lymphocyte reaction, such Oridonin plus LPS-treated DCs didn’t inhibit T cells proliferation and cytokine production. On the other hand, we found that Oridonin could directly suppress anti-CD3/CD28 antibodies-activated CD4+ T cells cytokine production. Based on the above results, we though that Oridonin may have the therapeutic ability to reduce allergic-specific immune responses in vivo. In therapeutic animal model of OVA-induced asthma, Oridonin treatment significantly attenuated the severe levels of airway hyper-responsiveness and the lung inflammation, as well as Th2 cytokines secretion in OVA-stimulated splenocytes. Additionally, the expression levels of OVA-specific IgE and IgG1 were reduced in these Oridonin-treated mice. Notably, the administration of Oridonin in enhanced the generation of CD25+ Foxp3+ CD4+ regulatort T cells (Treg). These findings indicated that Oridonin may inhibit the development of allergic asthma by increasing the number of CD25+ Foxp3+ CD4+ Treg cells in these mice. In the future, we hope that Oridonin can be development as a novel agent to treat allergic asthma.
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Liu, Yi-Ling, and 劉怡伶. "The immuno-modulatory effects of Notch ligand Delta4 in the induction of immune cells activation and differentiation." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/74876297286733634703.
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碩士臺北醫學大學
醫學科學研究所
97
The Notch pathway can support cell proliferation, differentiation, and apoptosis processes to influence the development and function of various organs. Dendritic cells (DCs) are professional antigen presenting cells (APCs), they can induce T cells activation and promote T cells differentiation by stimulating with antigen. Some data proved that Delta on APCs associated to stimulate Th1-type responses. However, the complex interaction of Notch signaling and molecular events underlying these processes of DCs and T cells remains controversial. To observe whether Delta4 plays the immune-modulatory role in immune cells, we generated Ad-Delta4 vector to address this question. However, we can’t detect the Delta4 protein expression in this system. Therefore, we futher used retro-Delta4 vector to transfect C2C12 cells. Unfortunately, the retro-Delta4-transfected C2C12 cells interfered the effect of Delta4 on DCs and was not suitable to be a adeptor to satumilate DCs. Finally, recombinant Delta4 protein was used as a tool to proceed the expiriments. The data showed that Jagged1, Delta1, and Delta4 mRNA expressions were increased in Delta4-pretreated DCs. However, the expressions of those Notch ligands were reduced in OVA-stumilated DCs in the presence of Delta4. In addition, IL-12 prodution was decreased and IL-10 secreation was increased in OVA-treated DCs with Delta4 stumilation. Forthermore, we observed that T cells prefered to differentiate to Th1 cells after high dose of Delta4 treatment. In contrast, low dose of Delta4 promoted Th2 cell development. In the future, we need to find a suitable cell line as a Delta4 overexpression adaptor to induced Th1-type cells differentiation and inhibit Th2-type cells generation. Therefore, Delta4 might be used as novel therapeutic reagent for asthma treatment.
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HO, Kuan-Tseng, and 何冠增. "Study on the modulatory effects of norisoboldine on immune effector cells and airway inflammation in a murine model of asthma." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/crcc6f.
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CHENG, CHIA-PI, and 鄭珈毘. "To Investigate The Immune Modulatory Effects And Mechanisms Of Decoy Receptor 3 And Small Molecule (NDMC101) In Rheumatoid Arthritis." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/44514884021501276121.
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博士國防醫學院
醫學科學研究所
100
Here we want to investigate the immunomodulatory effects and mechanisms of decoy receptor 3 (DCR3) and small molecule N-(4-chloro-2-fluorophenyl)-2- hydroxybenzamide (NDMC101) in an experimental model of rheumatoid arthritis. In methods, differentiation into osteoclast-like cells was examined by tartrate-resistant acid phosphatase (TRAP) staining and expression of osteoclast differentiation markers. Osteoclast activity was assessed by Pit formation assay. The mechanism of the inhibition was studied by biochemical analyses such as RT-PCR and immunoblotting. Besides, cell viability was determined by MTT assay. Cell apoptosis and signaling pathways were evaluated by immunoblotting and using flow cytometry. Collagen-induced arthritis (CIA) mice were administered test articles by gavages and treated with DCR3 by hydrodynamic injection to assess its efficacy. Then clinical, histological, and biochemical parameters were assessed to determine the effects of DCR3 and N-(4-chloro-2-fluorophenyl)-2-hydroxybenzamide (NDMC101) on synovial inflammation and bone erosion by hematoxlin and eosin staining and Enzyme-linked immunosorbent assay (ELISA). Furthermore, splenocytes and inguinal lymphocytes cell numbers, cell populations, and serum proinflammatory cytokines were analyzed by using flow cytometry. We found that in vitro, DCR3 inhibited Pam3CSK4 (Toll-like receptor 1/2 ligand)-induced B220+ B cells proliferation. DCR3 and NDMC101 inhibited RANKL-induced NF-κB activation and NFATc1 nuclear translocation in RAW264.7 and also inhibited RANKL-induced formation of TRAP+ multinucleated cells. Pit formation assay showed that DCR3 and NDMC101 significantly inhibited the bone-resorbing activity of mature osteoclasts. Moreover, DCR3 enhanced RANKL-induced Fas ligand expression and enhanced RANKL-induced cell apoptosis. DCR3 induced intrinsic cytochrome c and activated Caspase 9 apoptosis pathway. In vivo, DCR3 and NDMC101 significantly attenuated disease severity in collagen-induced arthritis (CIA) mice. DCR3 inhibited the volume of inguinal lymph nodes, numbers of CD19+ B cells, and population of CD4+ T cell which producing IFNγ, IL-4, IL-17A, and Foxp3. DCR3 treatment reduced the serum level of IL-6, total IgG2a, and CII specific IgG2a antibody. NDMC101 treatment reduced the serum proinflammatory cytokines TNFα and IL-1β. In conclusion, the data indicated that NDMC101 could more effectively inhibited RANKL-induced osteoclastogenesis than salicylic acid. Also, DCR3 could induce RANKL-stimulated monocyte apoptosis. In vivo, both molecules could attenuate collagen-induced arthritis in mice via suppressing proinflammatory cytokines. Moreover, DCR3 impaired peripheral lymphocytes activation and proliferation in lymphatic organ. Therefore, these might provide a new insight of remedy for rheumatoid arthritis.
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Chang, Ching Mao, and 張清貿. "The efficacy of Chinese herbal formula on Sjögren's syndrome and its mechanism of anti-oxidative and immune modulatory effects." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/v27p52.
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Papageorgiou, Marco. "The Immunomodulatory Effects of Methamphetamine." Thesis, 2020. https://vuir.vu.edu.au/41838/.
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The psychostimulant, METH causes central nervous system damage, along with short and long-term changes to the innate and adaptive immune system. METH was investigated for its impact(s) across a range of physiological contexts. Specifically, crystal METH was investigated for its immune-modulatory effects, in cells of the innate immunity, as well as gene expression modifications in the mouse colon using open- source gene ontology programs. In this regard, changes in differential gene expression and subsequent enrichment in gene ontology groups allowed for a deeper understanding of how METH impacts ontological pathways. Metagenomics was also employed to track changes to colon bacteria upon an escalating dose, followed by a withdrawal period. Together, results indicated that METH causes changes to some genes involved in innate immunity, and minor shifts to abundant bacterial species in the colon. Moreover, gene ontology networks showed several significantly up- and down-differentially regulated genes across functional, molecular and biological processes according to open-source software. Overall, this work represents a significant milestone in the amalgamation of bioinformatics, next-generation sequencing technology and metagenomic diversity profiling. Lastly, this work can initiate further research into how chronic METH use, and withdrawal could help construct models on weaving the relationship between mental health outcomes in METH users.
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Kuo, Fu-Hua, and 郭馥華. "The modulatory effect of silibinin on T cell immune responses." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/61043180292708594484.
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碩士國立臺灣大學
獸醫學研究所
96
Silibinin and silymarin have been used as herbal medicines to treat liver disease for a long time. Recent years, silibinin and silymarin has been commonly used as dietary supplements, as well as medicinal products in many countries, including Taiwan. To date, scientific evidence indicates that silibinin and silymarin possess potential biological activities, including immune modulation (i.e. effects on T cell reactivity and cytokine expression). Although the immunomodulatory activity of silibinin on T cell functionality has been documented, contrasting effects on the balance of T helper (Th)1/Th2 cell-mediated immunity were reported. Hence, the objective of the present study was to investigate the effect of silibinin on Th1/Th2 immune balance. Both animal models and cell culture experiments were employed to examine the influence of silibinin on T-dependent antibody production, T cell-mediated allergic airway responses, and cytokine expression. The results demonstrated that daily oral administration of silibinin for 3 days prior to ovalbumin (OVA) sensitization markedly enhanced the production of IFN-γ by splenocytes and the serum level of OVA-specific IgG2a in OVA-sensitized BALB/c mice. In contrast, the production of IL-4 and OVA-specific IgE and total IgE was attenuated. These finding indicates that silibinin administration polarizes the Th1/Th2 balance toward the Th1 direction. In the murine model of allergic airway responses induced by the challenge of OVA aerosol to OVA-sensitized mice, no significant effect on the airway allergic immune response by silibinin administration was observed; however, the steady state mRNA expression of IFN-γ in the lung tissues of OVA-sensitized and challenged mice was markedly enhanced by silibinin administration. Furthermore, the direct effect of silibinin on T cell-derived cytokine expression was also studied in vitro. Splenocytes obtained from OVA-sensitized mice were exposed to silibinin in culture and stimulated with OVA to induce cytokine production. The results showed that silibinin exposure significantly attenuated the production of both IFN-γ and IL-4 by OVA-stimulated splenocytes. These results demonstrated that the effects of silibinin on T cell cytokine expression between the employed in vivo and in vitro experimental settings are inconsistent. The present study utilized 3 experimental systems, including animal models and cell culture experiments, to examine the immunomodulatory effect of silibinin on T cells; however, the results on T cell cytokine expression are contrasting. The inconsistency of silibinin-mediated effects on the Th1/Th2 immune balance has been reported in the literature, suggesting a very complicated profile of silibinin influence on the immune system. It is noticed that, under the employed condition of cell culture studies, the effective concentration range of IV silibinin (5-10 μM) to affect Th1 (IFN-γ) and Th2 (IL-4) cytokine expression is lower than the concentrations used by many reports in the literature. Together with the demonstrated effects of silibinin on Th1/Th2 cytokine expression in murine models, it is apparent that T cells are a sensitive target for silibinin. Further studies to elucidate the mechanism of silibinin-mediated effects on cytokine expression are warranted.
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Hurinanthan, Vashka. "Immune modulatory effect of Dichrostachys cinerea, Carpobrotus dimidiatus, Capparis tomentosa and Leonotis leonurus." Thesis, 2009. http://hdl.handle.net/10321/455.
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Submitted in fulfillment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, 2009.Dichrostachys cinerea, Carpobrotus dimidiatus, Capparis tomentosa and Leonotis leonurus are all plants that are indigenous to South Africa. These plants are used in traditional medicine to treat various ailments. However, there is little or no scientific data to justify these traditional uses. Furthermore, it is difficult to reconcile traditional knowledge with scientific evidence because of the overwhelming targeting of signal-responsive systems by plant defensive compounds, multiple sites of action and the connectedness of the signaling pathways, which provide many cures and have pleiotropic effects. In order to evaluate the action spectrum of these plants, and validate its widespread use, this research evaluated the antibacterial, antioxidant, anti-inflammatory, anti-mosquito and immunomodulatory properties of these plants. Antimicrobial activity of the extract was determined by evaluating the bactericidal and fungicidal action using the agar disc diffusion assay. Anti-oxidative properties of the extracts were tested using the DPPH photometric assay. Anti-inflammatory properties were carried out using the 5-lipoxygenase assay. The larvicidal, repellency and insecticidal assay was determined against A.arabiensis. The safe use of these plant extracts was determined by evaluating toxicity, a brine shrimp lethality assay and an in vitro cell culture system using human myelogenous leukemia cell line. Potential carcinogenic activity was evaluated using the Ames Salmonella Mutagenecity assay. The immunomodulatory activity of the extracts on human peripheral blood mononuclear cells 6 was evaluated on freshly harvested lymphocytes using the MTT assay. Cytokine response was evaluated by measuring the secretion of interferon-gamma and interleukin-10. Elucidation of the B cells, T cells, activated T cells, CD 4+, CD 8+ and NK cells was performed by flow cytometry. The extracts showed anti-microbial activity against Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Salmonella typhimurium, Serratia marcescens, Bacillus cereus and Tricoderm sp. The highest activity was shown by methanolic and aqueous extracts of L. leonurus leaves followed by methanolic and aqueous extracts of D. cinerea. Extracts of C. tomentosa and D.cinerea demonstrated a higher degree of free radical scavenging than rutin, which was used as a standard indicating that these plants have strong antioxidant properties. None of the plants showed significant anti-inflammatory activity when compared to NDGA. In the anti-mosquito assays, the extracts showed strong repellency and insecticidal activity. L. leonurus extracts demonstrated the highest insecticidal and repellency activity against the mosquito, and was also found to cause ‗knockdown‘ and mortality. The extracts display no toxicity, cytotoxicity and mutagenicity. The immunological studies for immune modulation showed that the methanol extracts of these plants induce a Th1- predominant immune response because they significantly suppressed the secretion of IL-10 and augment IFN-γ production, which are hallmarks used to indicate a stimulation of the innate immune response. This study also provides new information, with respect to the potential use of these plants in producing a mosquito repellent and an immunostimulant.
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Lo, Modou Moustapha. "Characterisation of the immune modulatory effect of wild type Rift Valley fever virus strains." Doctoral thesis, 2010. http://hdl.handle.net/11858/00-1735-0000-0006-ADCE-F.
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Chiang, Yu-Bon, and 蔣友邦. "Effect of the dimerization of the Ganoderma microsporum protein GMI on its immune-modulatory function." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/14161518192087532402.
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碩士國立臺灣大學
生化科技學系
100
Lingzhi, a popular medicinal edible mushroom, has been used in Asia for the past 2000 years. The antitumoral and immune-stimulating effects of Lingzhi are attributed to polysaccharides, triterpenoids and fungal immunomodulatory proteins (FIPs). This study investigated GMI, a FIP cloned from Ganoderma microsporum and expressed in a methytrophic yeast, Pichia pastoris. The native form GMI is a noncovalently linked homodimer, and the monomer consists of 111 amino acids, with molecular weight of 12.4 kDa. In this study, three different point mutation GMIs here made at the sixth residue, from leucine (L) to cysteine (C), lysine (K) and phenylalanine (F) respectively, resulting of the mutant GMIs, GMI-L6C, GMI-L6K and GMI-L6F. These mutants and wild type GMI here used to study the role of dimerization in immune modulatory functions and protein structure. GMI-L6C is able to form a stable homodimer via the formation of a disulfide bond. GMI-L6K and GMI-L6F tend to be pure monomer due to the positive charge or steroic conformation. Based on the results of the immune cell assays and nanoparticle analyzer, homodimer form GMI-L6C is able to increase immune cells function, while the monomer form GMI-L6K and GMI-L6F are unable to enhance immune activity.
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葉怡玲. "The therapeutic effect and immune modulatory mechanism of IL-12 in an animal model of asthma." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/13773212455726237154.
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博士國立臺灣大學
免疫學研究所
91
There is strong evidence for the therapeutic effect of type 1 T helper cell (Th1)-related cytokines or reagents, such as IFN-g, IFN-a, IL-12 or CpG-motif oligodeoxynucleotide on airway inflammation. However, the mechanisms responsible for the therapeutic effect of Th1 cytokine have not yet been clarified. In order to study the therapeutic role of IL-12 on Der p1 sensitized mice, the interleukin-12 (IL-12) DNA plasmid as a therapeutic reagent was delivered intravenously into sensitized mice one day before aerosol challenge. The results demonstrated that intravenous delivery of IL-12 exerted no effect on anti-Der p1 IgG1, IgG2a and IgE antibody levels. Although IL-5 secretion level was suppressed by the treatment, the IL-4 or IFN-g secretion by splenocyte was not affected. Interestingly, IL-12 DNA plasmid could effectively inhibit eosinophilia and airway inflammation in vivo. It is noticeable that the level of eotaxin in BAL fluid also decreased. To further investigate the effect of Th1-related cytokines such as IL-12 or interferon-g (IFN-g) on the eotaxin level produced by lung cells, primary lung cell culture was established. The data suggested that IL-12 regulated airway inflammation by suppressing eotaxin secretion by lung tissue through an IFN-g independent mechanism. That might be the reason why IFN-g dependent or independent pathways both have been reported on IL-12 treatment. IL-18 is well known by its Th1 promoting effect when accompanying with IL-12. Dendritic cells (DCs) are the most potent antigen presenting cells for priming the naïve T helper cells. In order to prevent or treat airway inflammation, we used Ad-IL-12 and Ad-interleukin-18 (Ad-IL-18) infected dendritic cells to establish antigen-specific Th1 immune response in mice. Two experiment protocols were used including mice receiving cytokine expressing adenovirus infected DC seven days before allergen sensitization or six days before airway inhalation challenge respectively. The data showed that cytokine expressing adenoviruses modulated dendritic cells could alleviate airway inflammation in murine model of asthma. Ad-IL-12 infected DC suppressed eosinophilia but not IgE Ab level and airway hyperresponsiveness (AHR). By contrast, Ad-IL-12 and Ad-IL-18 co-infected DCs can enhance Th1-related immune response and suppress allergen-specific IgE level, eosinophilia and AHR. Further, intratracheal delivery of Ad-IL-12 infected DCs 6 days before aerosol challenge can alleviate eosinophilia and AHR. However, the therapeutic effect of Ad-IL-18 only or co-infected DCs was not very significant. In conclusion, IL-12, similar to IFN-g, could inhibit the eotaxin expression by lung cells. Bone marrow-derived dendritic cells infected with adenoviral vectors expressing IL-12 genes can promote the Th1 immune response in mouse (IgG2a). Furthermore, Ad-IL-12 and Ad-IL-18 infected dendritic cells could effectively enhance the anti-OVA IgG2a antibody and inhibit the anti-OVA IgE Ab level in serum and subsequently suppress airway inflammation. Late treatment with Ad-IL-12 infected DCs could alleviate the eosinophilia, AHR, eotaxin expression and enhance the IFN-g expression in BAL fluid. Interestingly, Ad-IL-18 alone induced IFN-g expression but it did not suppress eosinophilia efficiently when compared to those of Ad-IL-12 treated group. The application of IL-12 genes or cytokine genes modulated dendritic cells can decrease airway inflammation (Th2 immune response switch to Th1 type) but the long-term therapeutic effect needs further investigation.
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Huang, Hsin-Yi, and 黃馨儀. "Immune modulatory effect of a pure compound Isolated from Taxillus lonicerifolius var. lonicerifolius in murine model of asthma." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/18749921700134755819.
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碩士國立臺灣大學
口腔生物科學研究所
95
Allergic asthma is one of the most important Th2 lymphocyte-mediated chronic inflammatory airway disease worldwide, both in the developing and developed countries. In the patients of allergic asthma, it is well characterized by skewed polarization of helper T cell development toward type 2 T helper cell with increased production of interleukin 4 (IL-4) and IL-5. Asthma is characterized by airway eosinophils inflammation, mucus hypersecretion, bronchial hyperreactivity, the presence of high levels of allergen-specific IgE. There is strong evidence for the therapeutic effect of type 1 T helper-related cytokines or reagents, such as IFN-α, IFN-γ, IL-12 or CpG-motif oligodexynucleotide on airway inflammation. Dendritic cell are the most potent antigen presenting cells for priming the naïve T helper cells. Taxillus lonicerifolius var. lonicerifolius is one species of Loranthaceae in Taiwan. One pure compound (1400041), kindly providing by Pro. Leu,. isolated from the fresh T. lonicerifolius var. lonicerifolius was used in this study. In this study, we found stimulating BM-DC with 30μg/ml 1400041 could enhance production of IL-12 p40, IL-12 p70 and IL-10, as well as enhance cell surface expression of CD80 (B7.1), CD86 (B7.2), MHCI and MHCII molecule. In addition, the capacity for endocytosis was suppressed in BM-DC treated with 1400041. And, neutralization with antibody against Toll-like receptor（TLR）-4 inhibited the 1400041-induced production of IL-12 p40, suggest a crucial role for TLR-4 in signaling DC upon incubation with 1400041. In addition, treatment of BM-DC with 1400041 resulted in enhanced T cell-stimulatory capacity and increased T cell secretion of IFN-γ. Taken together, our in vitro data demonstrated that this 1400041 can effectively promote the activation and maturation of DC, suggesting that 1400041 may possess a potential in regulating immune responses. To test the effect of 1400041 in vivo, we further examined the effects of this compound on antigen-specific antibody and cytokine production. Immunization with ovalbumin (OVA) / 1400041 showed that the anti-OVA IgG2a levels were significantly increased and the anti-OVA IgE levels were significantly decreased compared with that of OVA-alone group. While 1400041 had no significantly effect on the anti-OVA IgG1 levels and induced the secretion of IFN-g, it inhibited the secretion of IL-4 and IL-5. The findings demonstrate that 1400041 could be used as adjuvant to induce Th1 immunity in BALB/c mice. In the future, we aim to study the therapeutic effect and immune modulatory mechanism of 1400041 in murine model of asthma. We expect this compound might be used as novel therapeutic reagent for asthma treatment.
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Su, Hsiao-Chiao, and 蘇筱喬. "The modulatory effect of IL-10 and IL-12 genes on immune cells and OVA-induced asthmatic mice." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/80153149791803769908.
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碩士臺北醫學大學
醫學科學研究所
96
The T helper type 2 cells (Th2) play a curial role in the initiation of allergic asthma. Asthma is characterized by reversible airway obstruction, eosinophilic infiltration, airway remodeling, mucus hypersecretion and airway hyperresponsiveness. In our studies, we use IL-10 or IL-12 expessing adenoviral vector to observe whether combination treatment can suppress the airway inflammation of allergic asthma. Firstly, we applied the Ad-IL-10/IL-12 to the bone marrow derived dendritic cells (DCs) and found such modified DCs expressed increased surface makers of CD 80, CD 86 and MHC class II. These modified DCs drived activated CD4+ T cells to differiate to specific T cell line. These special T cells secreted high levels of IL-10 and IFN-γ and suppressed the proliferation of effector T cells through cell-cell contact rather than the cytokine mediation. In the in vivo experiments, Ad-IL-10/IL-12 infected DCs were injected into OVA-induced asthmatic animal model to investigate whether modified DCs could prevent the development of asthma. The data showed that modified DCs suppressed the development of airway hyper-responsiveness, reduced airway inflammation and enhanced the expression of IL-10 and IFN-γ. We suggest that Ad-IL-10/IL-12 infected DCs can prevent the rise of asthma effectively. In addition, this modified DCs applied to the established asthmtic animal model and the same therapeutic effect was observed. In conclusion, we suggest that Ad-IL-10/IL-12 infected DCs induce a group of IL-10+ and IFN-γ+ T cells in vivo and such specific T cells can suppress the airway inflammation of asthmatic animal. Thus, such modified DCs may provide a more appropriate tool in future application of gene therapy for allergic diseases.
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Lo, Modou Moustapha [Verfasser]. "Characterisation of the immune modulatory effect of wild type Rift Valley fever virus strains / vorgelegt von Modou Moustapha Lo." 2010. http://d-nb.info/1009494813/34.
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HUANG, SHAO-CING, and 黃紹清. "Establishment of atopic dermatitis in mouse model and investigation of the immune modulatory effect of probiotics in this model." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/44723841668439943650.
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碩士國立中正大學
分子生物研究所
102
Atopic dermatitis (AD) is an allergic disease of skin and caused by environmental allergens. Differentiated type 2 helper T (Th2) cells arise while invasion of allergens in skin. A mouse model of atopic dermatitis, OVA-induced allergic skin, was investigated in previous study. Recently much researches reported that treatment of probiotics can modulate immune response and cause immunological homeostasis, even relieve allergy symptoms. However, administration of Bacillus natto increase and stabilize the normal flora resident in intestine although these related studies are rare. In this study we use the OVA-induced method to establish mouse model of AD, and feed mouse for mixed probiotics include Bifidobacterium bifidum, Bifidobacterium longum, Lactobacillus plantarum, and Lactobacillus rhamnosus or natto bacteria (Bacillus natto Sawamura) to investigate the therapeutic and immunological effects of mixed probiotic reagents or Bacillus natto in AD. We successfully established a mouse model of atopic dermatitis refer to previous study, and found that OVA-induced mice had much severe inflammatory response including the appearance of skin, increased invasive cell count in the skin, increased IgE concentration of serum, and the up-regulated Th2 related cytokine expression of splenocytes compared to the PBS-induced mice. Many studies had found that probiotics may increases the expression of Th1 cytokines and Treg cells in vivo, cause immunological homeostasis and even relieve allergy symptoms. However, in our study, treatment of mixed probiotics or Bacillus natto could not ameliorate inflammation including the appearance of the skin, invasive cell count in skin, IgE concentration of serum, and Th2 related cytokine expression compared to the mice of OVA-handling only. Further we analyzed the Th1 cytokine IFN-γ expression and regulatory T cells transcription factor FOXP3 expression and found there is no significant difference between these. Therefore, we use the mixed probiotic reagents and Bacillus natto in AD model, and cannot effectively modulate the immune response even to see any improvement of the disease.