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Dissertations / Theses on the topic 'Immunoassay Enzyme-linked immunosorbent assay'

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Published: 4 June 2021

Last updated: 12 February 2022

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1

Carroll, Andrea D. "Development of bead injection methodology for immunoassays /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/8599.

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2

Orchard, Robert Graham. "A flow-through enzyme-linked immunoassay for progesterone." The University of Waikato, 2007. http://hdl.handle.net/10289/2277.

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Bovine reproductive performance is one of the most important factors influencing dairy farm profitability. Present-day techniques for oestrus- and pregnancy-detection are unreliable and labour-intensive. Although measuring milk-progesterone at regular intervals allows the fertility status of a cow to be determined reliably, the labour cost of collecting and analysing samples is prohibitive. This project aimed to develop a progesterone sensing system that could be automated and integrated with the milking unit, thus minimising labour costs. The proposed system involved mixing the milk sample wi

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3

Campbell, Charles Nelson. "Separationless immunoassay and DNA sensing using wired enzyme based amperometric affinity electrodes /." Digital version:, 2000. http://wwwlib.umi.com/cr/utexas/fullcit?p9992761.

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4

de, Alwis Wathuthanthirige Uditha. "Analytical applications of chemically modified antibodies." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184601.

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The components involved in an immunoassay were investigated in order to improve the detection limits of the ELISA and to make the assay adaptable to a flow injection analysis (FIA) configuration. The goal being the total automation of the ELISA procedure which is long, tedious and has high standard deviation. The antibody purification and cleavage methods were studied with special emphasis on obtaining products with highest immunological activity. The antibody-enzyme coupling reactions using homobifunctional reagents and heterobifunctional reagents were studied in order to attempt the preparat

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5

Lövgren, Ulf. "Enzyme immunoassay in combination with liquid chromatography for sensitive and selective determination of drugs in biosamples." Lund : Dept. of Analytical Chemistry, Lund University, 1997. http://books.google.com/books?id=Ju1qAAAAMAAJ.

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6

Kiote-Schmidt, Chrissoula. "Etablierung eines kompetitiven enzymgekoppelten Immunoassays zum Nachweis eines kleinen Peptids in Serum- und Liquorproben." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-59192.

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7

Booth, Ronald A. Carleton University Dissertation Biology. "Development of cloth-enzyme immunoassays for the detection of E. coli O157 and verotoxin." Ottawa, 1996.

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8

Moberg, Ylva. "Evaluation of an equine-optimized enzyme-linked immunosorbent assay for the determination of serum insulin in canine and feline samples." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-354036.

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Background: Insulin is an important hormone for glucose homeostasis. It is released from β-cells in the endocrine pancreas as a response to increased concentrations of plasma glucose. The major effect of insulin is the facilitation of cellular uptake and storage of glucose as glycogen. Insulinomas are tumours that produce excessive amounts of insulin resulting in hypoglycaemia. The condition has been observed in dogs and cats and is often malignant. One part of establishing the diagnosis is confirmation of elevated concentrations of insulin in a hypoglycaemic sample. Aim: The aim of this study

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9

Hardy, Charles Thomas. "Evaluation and calibration of enzyme immunoassays for detecting antibody to the human immunodeficiency virus and other agents /." Thesis, Connect to this title online; UW restricted, 1993. http://hdl.handle.net/1773/9297.

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10

Kim, Sangkyung. "Redox cycling for an in-situ enzyme labeled immunoassay on interdigitated array electrodes." Diss., Available online, Georgia Institute of Technology, 2004:, 2004. http://etd.gatech.edu/theses/available/etd-08192004-105039/unrestricted/kim%5Fsangkyung%5F200412%5Fphd.pdf.

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Thesis (Ph. D.)--Biomedical Engineering, Georgia Institute of Technology, 2005.<br>Hesketh, Peter, Committee Chair ; Edmondson, Dale, Committee Member ; Frazier, Albert, Committee Member ; Hunt, William, Committee Member ; Janata, Jiri, Committee Member. Includes bibliographical references.

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11

McDuffee, Emily. "A new immunoassay for quantification of a novel cancer antigen in serum and immunostaining of carcinoma tissues and cultured cells revealing the antigenic cellular location." [Johnson City, Tenn. : East Tennessee State University], 2002. http://etd-submit.etsu.edu/etd/theses/available/etd-1106102-175606/unrestricted/McDuffeeE112102a.pdf.

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12

Bahlmann, Arnold. "Entwicklung und Validierung eines Enzyme-linked Immunosorbent Assays (ELISA) für die Quantifizierung von Carbamazepin in Abwasser, Oberflächenwasser und Trinkwasser." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16729.

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Ein kompetitiver ELISA (Enzyme-linked Immunosorbent Assay) für den Nachweis von Carbamazepin (CBZ) mit einer Bestimmungsgrenze von ca. 30 ng/L wurde entwickelt und validiert. Dieser in Gewässern häufig auftretende anthropogene Marker wurde anschließend in einer Vielzahl an Proben aus Abwässern, Oberflächengewässern und Trinkwässern nachgewiesen. Der ELISA zeigte eine exzellente Präzision und erbrachte in allen Matrizes geringfügig höhere Analysenergebnisse als die Referenzmethode HPLC-MS/MS. Die beständige Überbestimmung der CBZ-Konzentration in Höhe von ca. 7 % konnte auf die Präsenz von Ceti

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13

Wesolowski, Eva. "Monoclonal antibody enzyme-linked immunosorbent assay for carcinoembryonic antigen." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66230.

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14

Nording, Malin. "Rapid sample preparation and bioanalytical techniques for efficient screening of organic pollutants in the environment." Doctoral thesis, Umeå universitet, Kemi, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-842.

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Large numbers of samples often need to be prepared and analysed in surveys of organic pollutants in the environment, but while the methods commonly used in such surveys can provide abundant detail they are generally costly, time-consuming and require large amounts of resources, so there is a need for simpler techniques. The work underlying this thesis assessed the potential utility of more convenient sample preparation and bioanalytical techniques for rapidly screening various environmental matrices that could be useful complements to higher resolution methods. Initially, the utility of a simp

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15

de, Oliveira Felipe Marques Souza. "Development and Application of Proximity Assays for Proteome Analysis in Medicine." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-334536.

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Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. In paper I, we present an adaptation of the solid-phase proximity ligation assay (SP-PLA) for the detection of post-translational modification of proteins (PTMs). The assay was adapted for

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16

Kim, In Soo. "A quantitative enzyme linked immunosorbent assay for polychlorinated biphenyls in transformer oil." Thesis, Cranfield University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323838.

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17

Meindel, Mandy J. "Enzyme-linked immunosorbent assay to measure serum ferritin in toucans (Ramphastidae sp.)." Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/18896.

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Master of Science<br>Department of Diagnostic Medicine/ Pathobiology<br>Lisa M. Pohlman<br>Background: Iron storage disease has proven to be a serious health concern for captive toucans. Physiologic mechanisms to efficiently extract iron from naturally iron-deficient diets appear the likely cause of iron overload when fed iron-sufficient diets in captivity. Iron overload can result in diabetes, heart failure, and even death. Serum ferritin concentrations are considered the most reliable screening tool to predict total body iron stores in many species, but an assay has not been available to

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18

Kwok, Siu Kwong Alan. "Enzyme-linked immunosorbent assay development for advanced glycation end products and brochocin-C." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/mq22621.pdf.

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19

Leung, Sau-man Sally. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2337309X.

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20

Nitaya, Poopyruchpong Vithoon Viyanant. "Detection of opisthorchis viverrini infection by enzyme-linked immunosorbent assay using partially purified antigens /." abstract, 1988. http://mulinet3.li.mahidol.ac.th/thesis/2531/31E-Nitaya-P.pdf.

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21

Murphy, Jamie Ellen. "Development and evaluation of a sarcocystis neurona-specific IgM capture enzyme linked immunosorbent assay." The Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1399903151.

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22

Östling, Jeanette. "An Approach to Improve the Detection System of a Diagnostic Enzyme-Linked Immunosorbent Assay." Thesis, KTH, Skolan för bioteknologi (BIO), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-214620.

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23

梁秀雯 and Sau-man Sally Leung. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970096.

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24

Sheehan, C. P. "The application of the enzyme-linked immunosorbent assay (ELISA) to ABH grouping in forensic science." Thesis, University of Surrey, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381661.

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25

Cheow, Lih Feng. "Development of an Enzyme-Linked ImmunoSorbent Assay (ELISA) with Enhanced Sensitivity in a Nanofluidic System." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/55148.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 66-68).<br>Experimental studies were performed to evaluate the kinetics and equilibrium binding constants of biomolecules in nanofluidic channels. Binding events in the nanochannel were detected using electrical and fluorescence methods. We concluded that antibody-antigen binding constants in nanochannels were similar to experiments performed in microtiter plates at low antigen concentrations; howev

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26

Lupica, Samuel J. "Nitrate Toxicity to Common Carp Measured Noninvasively by Novel Enzyme-linked Immunosorbent Assay for Cortisol." University of Toledo Health Science Campus / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1226956326.

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27

Botha, Elizabeth Magdelena. "Molecular characterization of South African lineage II West Nile virus isolates ltime PCR assay." Pretoria : [s.n.], 2008. http://upetd.up.ac.za/thesis/available/etd-06122008-130924/.

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28

Leelayawat, Chanvit. "Evaluation of antibody elution techniques using enzyme-linked antiglobulintest (ELAT) /." abstract, 1986. http://mulinet3.li.mahidol.ac.th/thesis/2529/29E-Chanvit-L.pdf.

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29

Gossen, Ulrike. "Indikation der Bodenqualität mittels immunologischer Quantifizierung der Enzymkonzentration am Beispiel der Urease in schwermetallkontaminierten Böden /." Großbeeren [u.a.] : IGZ, 2002. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=010272469&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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30

Lack, Pamela. "Bestimmung von Immunglobulin G und M im Serum neugeborener Kälber während der ersten zehn Lebenstage unter besonderer Berücksichtigung des Fütterungsregimes." Giessen : VVB Laufersweiler, 2006. http://geb.uni-giessen.de/geb/volltexte/2007/4107/index.html.

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31

Liu, Huiqing. "Characterization of two-component organothiol mixed monolayers on gold and quantification of nonspecific adsorption on mixed SAM biosensor platforms using electrochemical enzyme immunology." Auburn, Ala., 2007. http://repo.lib.auburn.edu/2007%20Spring%20Theses/LIU_HUIQING_29.pdf.

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32

Hitt, John Michael 1952. "DETERMINATION OF THE EFFICACY OF THE ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) IN CHARACTERIZING CROTALUS SNAKE VENOM AT THE SPECIES LEVEL." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/276352.

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33

Tse, Maying Tsemay, and 謝美盈. "Evaluation of three commercially available influenza A type-specific blocking enzyme-linked immunosorbent assays for seroepidemiologicalstudies of influenza A virus infection in pigs." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B4827382X.

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The emergence of the pandemic H1N1 2009 virus of swine-origin and its transmission back to swine highlighted the need for global surveillance of swine influenza. Serology can help to address the epidemiological situation of influenza infection. Since typical serology tests such as hemagglutination inhibition or microneutralization assays are subtype and partially virus-lineage specific, it is important to select appropriate viral antigens for such studies. A poorly chosen panel of antigens will lead to underestimation of the seroprevalence. The choice of well-matched antigen is difficult i

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34

Jahn, Carsten. "Analytik cuticulagebundener Rückstände des Fungicids Chlorthalonil unter besonderer Berücksichtigung immunchemischer Nachweisverfahren /." Aachen : Shaker, 2000. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=009061215&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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35

Eggemann, Gabriele. "Untersuchungen zum Vorkommen von Chlamydieninfektionen in Zuchtsauenbeständen und deren Bedeutung für das Fruchtbarkeitsgeschehen /." Giessen : Köhler, 1999. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=008937022&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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36

Leitch, Robert A. "Analysis of immunodominant epitopes of Neisseria gonorrhoeae lipopolysaccharide using a competitive inhibition enzyme linked immunosorbent assay." Thesis, University of Ottawa (Canada), 1985. http://hdl.handle.net/10393/4930.

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37

Sattler, Tatjana, Eveline Wodak, Sandra Revilla-Fernández, and Friedrich Schmoll. "Comparison of different commercial ELISAs for detection of antibodies against porcine respiratory and reproductive syndrome virus in serum." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-158536.

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Background: In recent years, several new ELISAs for the detection of antibodies against the porcine reproductive and respiratory disease virus (PRRSV) in pig serum have been developed. To interpret the results, specificity and sensitivity data as well as agreement to a reference ELISA must be available. In this study, three commercial ELISAs (INgezim PRRS 2.0 - ELISA II, Priocheck® PRRSV Ab porcine – ELISA III and CIVTEST suis PRRS E/S PLUS - ELISA IV, detecting PRRSV type 1 antibodies) were compared to a standard ELISA (IDEXX PRRS X3 Ab Test - ELISA I). The serum of three pigs vaccinated with

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38

Johnson, Raymond Camille Joseph. "Detection of nepoviruses by ELISA in tissue-cultured and field-grown grapevines." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27968.

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The detection by serology of nematode-transmitted polyhedral viruses (nepoviruses) in grapevines is often unreliable. Nepoviruses were detected by enzyme-linked immunosorbent assay (ELISA) in tissue-cultured and field-grown grapevines. Nepovirus detection in in vitro plants (plantlets) was affected by virus distribution and growth room temperature. The reliability of virus detection in field-grown grapevines was improved when modified grinding buffers were used. Arabis mosaic virus (AMV) was detected by ELISA, for the first time, in in vitro grapevines initiated from field-and screenhouse-gro

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39

Dell, Kerstin. "Entwicklung eines indirekten ELISA zum serologischen Nachweis von Opisthorchis felineus (Rivolta, 1884) und Metorchis bilis (Braun, 1790) (Trematoda: Opisthorchiidae) beim Fuchs : mit einem Beitrag zu den Leberenzymaktivitäten bei experimenteller Opisthorchiidose /." Friedland : Bielefeld, 2001. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=009538526&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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40

Bates, Kimberly M. "The biology of dictyocaulus viviparus in Missouri /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841262.

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41

Livingston, Robert Schaad. "Helicobacter hepaticus infections in mice : serodiagnosis by enzyme-linked immunosorbent assays and characterization of the mucosal immune response /." free to MU campus, to others for purchase, 1999. http://wwwlib.umi.com/cr/mo/fullcit?p9946275.

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42

Kellerer, Thomas Georg. "Entwicklung von immunochemischen und PCR-Methoden zum qualitativen Nachweis von Tilletia-Arten und Ustilago nuda in Saatgut." kostenfrei, 2009. https://mediatum2.ub.tum.de/node?id=739743.

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43

Roßkopf, Ute. "Validierung der Wirksamkeitsprüfung für Clostridium tetani Impfstoffe ad usum veterinarium durch den direkten Nachweis von Tetanus-Antitoxin im Zieltier mittels ELISA." Giessen : VVB Laufersweiler, 2007. http://geb.uni-giessen.de/geb/volltexte/2007/4469/index.html.

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44

Shoha, Louis M. "An immunoassay to detect antibodies against endodontic medicaments a thesis submitted in partial fulfillment ... endodontics /." 1989. http://books.google.com/books?id=KIc9AAAAMAAJ.

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45

Widmann, Andrea A. "Development of an enzyme immunoassay using whole plasma to determine progesterone concentrations during early pregnancy in the mare." Thesis, 1991. http://hdl.handle.net/1957/37384.

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46

Yeh, Li-Chun, and 葉俐君. "Development of Amoxicillin Enzyme-Linked Immunosorbent Assay." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/66506324246340310706.

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碩士<br>國立中興大學<br>獸醫學系<br>92<br>The residues of β-lactam antibiotics in consumable meat and milk products pose potential risks for public health because of their propensity to induce allergic reactions in individuals and the increasing incidence of microbial resistance against these compounds. Amoxicillin is a β-lactam antibiotics widely used in human and veterinary clinical medicine. The aim of this study was to develop an enzyme -linked immunosorbent assay (ELISA) suitable for detection of amoxicillin in biological fluids. Amoxicillin polyclonal antibody was raised from rabbits immu

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47

Huang, Hann-shyang, and 黃漢翔. "Development of enzyme-linked immunosorbent assay for dexamethasone." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/17669407357735364037.

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碩士<br>國立中興大學<br>獸醫學系<br>88<br>A simple ELISA was developed for the detection of dexamethasone in equine urine, serum and milk. Dexamethasone oxime was prepared by refluxing with -aminooxy acetic acid in ethanol, and was conjugated to bovine serum albumin (BSA) by mixed anhydride method. Anti-dexamethasone serum was obtained after five immunizations. Enzyme conjugate was produced with oxime and horseradish peroxidase (HRP). Checkerboard method was used to determine optimal concentrations of antibodies and enzyme conjugate. A standard curve was established suggesting that it is a competitive im

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48

Yi-Te, Lin, and 林奕德. "Study of Design of Enzyme-Linked -Immunosorbent-Assay Biochip." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/87331062443787556755.

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49

Ho, Hsiao-Ching, and 侯曉晴. "Study of Protein Chips for Enzyme-Linked Immunosorbent Assay." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/496uk5.

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碩士<br>國立成功大學<br>工程科學系碩博士班<br>90<br>The goal of the study is to design and fabricate a tumor marker chip by using the techniques of MEMS. Apply enzyme-linked immunosorbent assay (ELISA) on the chip for the analysis of tumor marker in biological fluids. There are many advantages, including low reagent consumption, lowsample volume and waste production, faster response time, and feasibility to operate as a point-of-care testing. The contents of the study have two parts. One is to design and fabricate the reaction area of the chip. The optimization of the chip was performed analyzing the parameter

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50

蘇翰農. "Development of enzyme-linked immunosorbent assay (ELISA) for clenbuterol." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/55671671701643133563.

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碩士<br>國立中興大學<br>獸醫學系<br>90<br>Clenbuterol is one kind of β2 selective agonists. It binds withβ2 receptors then via series of signal transductions activating protein kinase to make pharmalogical effect. Clenbuterol clinically uses as bronchodilators by enhancing the clearance of mucosal cilliary, but it improves the carcass and lowers the feed efficacy. Illegal abusement using as growth promoter is common now. An ELISA was developed for the detection of clenbuterol in diversity. The clenbuterol derived diazotized clenbuterol and was conjugated with HSA to produce antigen. Anticlenbuterol serum

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