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Journal articles on the topic 'Immunoassay Enzyme-linked immunosorbent assay'

Author: Grafiati

Published: 4 June 2021

Last updated: 12 February 2022

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1

Gan, Stephanie D., and Kruti R. Patel. "Enzyme Immunoassay and Enzyme-Linked Immunosorbent Assay." Journal of Investigative Dermatology 133, no. 9 (2013): 1–3. http://dx.doi.org/10.1038/jid.2013.287.

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2

Lequin, Rudolf M. "Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)." Clinical Chemistry 51, no. 12 (2005): 2415–18. http://dx.doi.org/10.1373/clinchem.2005.051532.

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Abstract This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient sampl

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3

Dasgupta, Amitava, Edward Kang, Margaret Olsen, Jeffrey K. Actor, and Pradip Datta. "Interference of Asian, American, and Indian (Ashwagandha) Ginsengs in Serum Digoxin Measurements by a Fluorescence Polarization Immunoassay Can Be Minimized by Using a New Enzyme-Linked Chemiluminescent Immunosorbent or Turbidimetric Assay." Archives of Pathology & Laboratory Medicine 131, no. 4 (2007): 619–21. http://dx.doi.org/10.5858/2007-131-619-ioaaai.

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Abstract Context.—Ginsengs are widely used by the general population. These herbs interfere with serum digoxin measurement using the fluorescence polarization immunoassay. Objective.—To assess potential interference of different ginsengs (Asian, American, and Indian, also known as Ashwagandha) in vitro and in vivo in a mouse model by using a new enzyme-linked chemiluminescent immunosorbent digoxin assay and an existing turbidimetric assay. Comparisons were made with the fluorescence polarization immunoassay. Design.—Aliquots of drug-free serum pools were supplemented with ginseng and apparent

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4

Sathe, Manisha, Shruti Srivastava, Sumit Agrawal, and Ramrao Ghorpade. "Effect of Spacer and the Enzyme-Linked Immunosorbent Assay." Defence Science Journal 66, no. 5 (2016): 471. http://dx.doi.org/10.14429/dsj.66.10700.

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The effect of spacers and the enzyme-linked immunosorbent assay (ELISA) formats on the functional parameters of assays such as lower detection limit, inhibitory concentration at 50 per cent (IC50), and specificity were studied. Enzyme conjugates having hydrophobic and hydrophilic spacers were prepared using O-isopropyl methylphosphonic acid (IMPA) and horseradish peroxidase (HRP) as an enzyme label. Comparison was made with reference to enzyme conjugate without any spacer. The present investigation revealed that the presence of a hydrophilic spacer in the enzyme conjugate significantly improve

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5

Vítková, M., Z. Macková, R. Koblovská, and O. Lapčík. "Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants." Czech Journal of Food Sciences 22, SI - Chem. Reactions in Foods V (2004): S199—S202. http://dx.doi.org/10.17221/10659-cjfs.

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The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4’ via a carboxy methyl spacer. The developed ELISAs are highly specific, I<sub>50</sub> values of the standard curves range between 0.3–1.2 ng/ml. The cross reactivities to other isoflavones are in acceptable range and the interference of non-isoflavonoid

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6

Du, Pengfei, Maojun Jin, Lihua Yang, et al. "A rapid immunomagnetic-bead-based immunoassay for triazophos analysis." RSC Advances 5, no. 99 (2015): 81046–51. http://dx.doi.org/10.1039/c5ra15106f.

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7

Heinegård, D., A. Björne-Persson, L. Cöster, et al. "The core proteins of large and small interstitial proteoglycans from various connective tissues form distinct subgroups." Biochemical Journal 230, no. 1 (1985): 181–94. http://dx.doi.org/10.1042/bj2300181.

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Large and small proteoglycans were separately isolated from a number of connective tissues and compared to determine the extent of structural similarity. This was studied by enzyme-linked immunosorbent assays and by the peptide patterns obtained when 125I-labelled proteoglycans were digested with trypsin. All the large proteoglycans, i.e. from tendon, sclera, cartilage and aorta, appear to contain the structure typical for the hyaluronic acid-binding region, both shown by enzyme-linked immunosorbent assay and by content of peptides unique for this region. These proteoglycans also share other s

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8

Radhakrishnan, Jeejabai, Rovi Origenes, Gina Littlejohn, et al. "Plasma Cytochrome c Detection Using a Highly Sensitive Electrochemiluminescence Enzyme-Linked Immunosorbent Assay." Biomarker Insights 12 (January 1, 2017): 117727191774697. http://dx.doi.org/10.1177/1177271917746972.

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Background: Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury. Methods and results: We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad dynamic range (2-1200 ng/mL in humans and 5-500 ng/mL in rat) and high assay reproducibility (inter-assay coefficient <6% in humans and <10% in rat). In patients after blunt trauma, plasma cytochrome c directly correlated with injury severity. In rats after cardiac resuscitation, plasma cytochr

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9

Ehm, M., and A. Kappel. "Immunoassays for diagnosis of coagulation disorders." Hämostaseologie 30, no. 04 (2010): 194–201. http://dx.doi.org/10.1055/s-0037-1619055.

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SummaryImmunoassays play a pivotal role in the clinical laboratory. In the coagulation section of the laboratory, they are used as an aid for diagnosis of deep vein thrombosis or pulmonary embolism, thrombophilia screening, or detection of coagulation factor deficiencies, respectively. Enzyme-linked immunosorbent assay (ELISA) and latex agglutination immunoassay technologies are currently most widely used, while Luminescent Oxygen Channeling Immuno - assay (LOCI®) and other chemiluminescencebased immunoassays are emerging technologies for the coagulation laboratory. However, not all immunoassa

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10

van der Ende, A., R. W. M. van der Hulst, P. Roorda, G. N. J. Tytgat, and J. Dankert. "Evaluation of Three Commercial Serological Tests with Different Methodologies To Assess Helicobacter pyloriInfection." Journal of Clinical Microbiology 37, no. 12 (1999): 4150–52. http://dx.doi.org/10.1128/jcm.37.12.4150-4152.1999.

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The sera of 142 Helicobacter pylori-positive and 32H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and the ELISA are more sensitive than the chemiluminescent enzyme immunoassay (P < 0.05). The chemiluminescent enzyme immunoassay has the advantage that it is fully automated.

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Andreeva, I. P., N. T. Vorobyeva, L. I. Vinnitsky, S. S. Bogush, E. M. Gavrilova, and A. M. Egorov. "Enzyme-linked immunosorbent assay for determination of cyclosporin a in whole blood." Biomeditsinskaya Khimiya 57, no. 5 (2011): 554–61. http://dx.doi.org/10.18097/pbmc20115705554.

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A test-system based on enzyme-linked immunosorbent assay (ELISA) for quantitative determination of cyclosporin A (CSA) in human whole blood has been developed. The detection limit of the method was 25 ng/ml, the linearity of the method in the concentration range 60-1400 ng/ml varied from 94 to 105%, the variation coefficient did not exceed 8%. The novel method exhibited good correlation with radio-immunoassay and polarization fluoroimmunoassay methods; the linear regression coefficients were 0.965 and 0.983, respectively. The developed test system is stable for at least 9 months when stored at

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12

Kudryashova, A. M., A. G. Galstian, E. B. Faizuloev, et al. "DETECTION OF ADENOVIRUS ANTIGEN BY A SURFACE-ENHANCED RAMAN SCATTERING ENZYME-LINKED IMMUNOSORBENT ASSAY." Journal of microbiology epidemiology immunobiology 1, no. 3 (2019): 25–31. http://dx.doi.org/10.36233/0372-9311-2018-3-25-31.

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Aim. Study of the possibility of adenovirus antigen detection by recording of surface-enhanced Raman scattering (SERS) spectra of enzyme oxidized product of 3,3',5,5'-tetramethylbenzidine. Materials and methods. Clinical fecal samples containing adenoviruses, group A rotaviruses, noroviruses and healthy children samples, as well as laboratory strains of adenoviruses with a titer of 5 — 6 lg TCD50/ml were used. Sandwich immunoassay was used, the Raman spectra were recorded by a Raman spectrometer (532 nm) after incubation with silver nanoparticles. Results. The concordance of the adenovirus det

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13

Rittenburg, James H., Alexandra Adams, John Palmer, and John C. Allen. "Improved Enzyme-Linked Immunosorbent Assay for Determination of Soy Protein in Meat Products." Journal of AOAC INTERNATIONAL 70, no. 3 (1987): 582–87. http://dx.doi.org/10.1093/jaoac/70.3.582.

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Abstract An Indirect, Competitive Enzyme-Linked Immunosorbent Assay (Elisa) Has Been Developed For Quantitation Of Soy Protein In Meat Products. The Methodology Allows Rapid Aqueous Extraction Of Meat Samples Into A Liquid Form Suitable For Assay. The Assay Is Highly Specific For Soy Protein And Is Designed To Measure Soy Protein Levels Between 1 And 10% Of The Wet Weight Of The Sample. Standardized, Stabilized Reagents For Carrying Out The Procedure Are Commercially Available In A Kit. The Analysis, Including Sample Preparation, Can Be Completed Within A Workday, And The Actual Immunoassay In

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14

Kodama, Takanari, Satoshi Ichiyama, Kumiko Sato, Toshi Nada, and Nobuo Nakashima. "Evaluation of a Membrane Filter Assay System, Ortho HCV Ab Quik Pack, for Detection of Anti-Hepatitis C Virus Antibody." Journal of Clinical Microbiology 36, no. 5 (1998): 1439–40. http://dx.doi.org/10.1128/jcm.36.5.1439-1440.1998.

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A simple membrane immunoassay assay system, Quik Pack, for the detection of hepatitis C virus antibody was compared with two enzyme-linked immunosorbent assays (ELISAs) in a study of 600 serum samples. Quik Pack exhibited excellent sensitivity and specificity: 96.0 and 99.7%, respectively, versus the ELISA-2 and 99.7 and 99.4%, respectively, versus the ELISA-3.

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15

Numazaki, K., S. Chiba, T. Moroboshi, T. Kudoh, T. Yamanaka, and T. Nakao. "Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies against Chlamydia trachomatis." Journal of Clinical Pathology 38, no. 3 (1985): 345–50. http://dx.doi.org/10.1136/jcp.38.3.345.

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16

Wen-Tao, Xu, Huang Kun-Lun, Deng Ai-Ke, and Luo Yun-Bo. "Enzyme linked immunosorbent assay for PAT protein detection in genetically modified rape." Chinese Journal of Agricultural Biotechnology 3, no. 3 (2006): 177–81. http://dx.doi.org/10.1079/cjb2006106.

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AbstractWe have developed and applied an immunoassay method to detect genetically modified (GM) rape containing phosphinothricin acetyltransferase (PAT). The purified PAT was identified by Western blotting and enzymic activity analysis. The polyclonal antibody against purified PAT protein was obtained and purified by both a saturated ammonium sulphate method and protein A-Sepharose 4B. The sensitivity and cross-reactivity of the polyclonal antibody has been demonstrated in an enzyme-linked immunosorbent assay (ELISA). The result of the ELISA for antiserum sensitivity was about 2×10−5mg/ml and

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17

Lyu, Zhaoyuan, Shichao Ding, Nan Zhang та ін. "Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of Aβ 1-40: A Biomarker of Alzheimer’s Disease". Research 2020 (19 жовтня 2020): 1–11. http://dx.doi.org/10.34133/2020/4724505.

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Single-atom nanozymes (SANs) possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity. By introducing SANs into immunoassay, limitations of ELISA such as low stability of horseradish peroxidase (HRP) can be well addressed, thereby improving the performance of the immunoassays. In this work, we have developed novel Fe-N-C single-atom nanozymes (Fe-Nx SANs) derived from Fe-doped polypyrrole (PPy) nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40. Result

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18

Masters, A. M., B. Samarasinghe, M. J. Kalkhoven, G. L. den Hollander, and D. G. Palmer. "Improvements to the immunoassay for detection of Rathayibacter toxicus in hay." Crop and Pasture Science 62, no. 6 (2011): 523. http://dx.doi.org/10.1071/cp10337.

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An improved protocol for the previously described enzyme-linked immunosorbent assay for Rathayibacter toxicus in hay is described. The improvements were driven mainly by the export hay industry requirement of same-day turnaround for testing of hay extracts. The preparation of hay extracts was shortened by 8 h. The time for adding samples to the enzyme-linked immunosorbent assay plates was shortened by the use of sample tubes with penetrable stoppers combined with specially designed racks. The monoclonal antibody used in the original protocol was purified and conjugated to horseradish peroxidas

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19

Kavanagh, Owen, Mary K. Estes, Amanda Reeck, et al. "Serological Responses to Experimental Norwalk Virus Infection Measured Using a Quantitative Duplex Time-Resolved Fluorescence Immunoassay." Clinical and Vaccine Immunology 18, no. 7 (2011): 1187–90. http://dx.doi.org/10.1128/cvi.00039-11.

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ABSTRACTA quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

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20

Liu, Zhenjiang, Kewei Ren, Ming Li, Jiagao Wang, Jianfan Sun, and Daolin Du. "A new residue method for the determination of flonicamid in agricultural and environmental samples using enzyme immunoassay systems." RSC Advances 6, no. 42 (2016): 35842–46. http://dx.doi.org/10.1039/c5ra27425g.

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21

Kunishima, ShinjI, Klyotaka Hayashi, Sentaro Kobayashi, Tomokl Naoe, and Ryuzo Ohno. "New enzyme-linked immunosorbent assay for glycocalicin in plasma." Clinical Chemistry 37, no. 2 (1991): 169–72. http://dx.doi.org/10.1093/clinchem/37.2.169.

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Abstract A new sandwich-type enzyme-linked immunosorbent assay for quantifying glycocalicin, a proteolytic fragment of platelet membrane glycoprotein Ib, is described. The assay is based on the use of two monoclonal antibodies raised against glycoprotein Ib and involves the avidin-biotin technique. The detection limit is 7 micrograms/L and the range of glycocalicin determined in plasma is 0.01 to 1 mg/L. Assay time is 2 h. The intra-assay CV ranged from 3.6% to 5.2%, the interassay CV from 5.4% to 8.0%. Analytical recovery of purified glycocalicin added to a plasma pool averaged 96%. In 36 hea

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22

Guimarães, M. C., B. J. Celeste, and E. L. Franco. "Evaluation of dot enzyme-linked immunosorbent assay for mucocutaneous leishmaniasis and comparison with microplate enzyme immunoassay." Journal of Clinical Microbiology 24, no. 3 (1986): 364–67. http://dx.doi.org/10.1128/jcm.24.3.364-367.1986.

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23

Wodzig, K. Will H., Maurice M. A. L. Pelsers, Ger J. van der Vusse, Werner Roos, and Jan F. C. Glatz. "One-Step Enzyme-Linked Immunosorbent Assay (ELISA) for Plasma Fatty Acid-Binding Protein." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 34, no. 3 (1997): 263–68. http://dx.doi.org/10.1177/000456329703400307.

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To allow a more rapid determination of heart-type fatty acid-binding protein (FABP) concentration in plasma a direct non-competitive (sandwich-type) ELISA was developed which uses high-affinity monoclonal antibodies to FABP. Total performance time of the one-step immunoassay is 45min. The standard curve was linear between 0·2–6μg/L, and the within-run and between-run coefficients of variations were below 6 and 11%, respectively. The serum FABP concentration measured in 79 healthy individuals was 1·6 (0·8) [mean (SD), range 0·3–5·0] μg/L. The assay can be used for rapid plasma or serum FABP mea

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Wang, Bing, Jincui Gu, Boyi Chen, et al. "Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool." Journal of Analytical Methods in Chemistry 2018 (June 5, 2018): 1–9. http://dx.doi.org/10.1155/2018/2641624.

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The identification of ancient wool is of great importance in archaeology. Despite lots of meaningful information can be achieved by conventional detection methods, that is, light and electron microscopy, spectroscopy, and chromatography, the efficacy is likely to be limited in the detection of ancient samples with contamination or severe degradation. In this work, an immunoassay was proposed and performed for the identification of ancient wool. First, a specific antibody, which has the benefits of low cost, easy operation, and extensive applicability, was developed directly through immunizing

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Paugam, A., J. Sarfati, R. Romieu, M. Viguier, J. Dupouy-Camet, and J. P. Latgé. "Detection of Aspergillus Galactomannan: Comparison of an Enzyme-Linked Immunoassay and a Europium-Linked Time-Resolved Fluoroimmunoassay." Journal of Clinical Microbiology 36, no. 10 (1998): 3079–80. http://dx.doi.org/10.1128/jcm.36.10.3079-3080.1998.

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With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an enzyme-linked immunosorbent assay using a peroxidase-conjugated detector antibody. No increase in the sensitivity of the detection of GM standards was seen with the europium-based fluoroimmunoassay.

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Kruhlik, A. S., D. V. Tsaulouski, T. V. Mirantsova, O. L. Sharko, and V. V. Shmanai. "Enzyme immunoassay of vitamin d3 metabolites." Proceedings of the National Academy of Sciences of Belarus, Chemical Series 56, no. 2 (2020): 197–205. http://dx.doi.org/10.29235/1561-8331-2020-56-2-197-205.

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We report herein improved version of the synthesis of hapten based on cholecalciferol and its active metabolite 25-hydroxycholecalcalferol. The methodology of obtaining high-molecular immunogenic conjugates of vitamin D3 derivatives with bovine serum albumin and horseradish peroxidase conjugates for direct ELISA was optimized. Immunisation of rabbits was carried out and polyclonal antibodies to 25-hydroxycholecalceferol were obtained and tested in an enzyme-linked immunosorbent assay. To improve the accuracy of the method, the sample preparation procedure was optimized, including the release o

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Candlish, Alan A. G., William H. Stimson, and John E. Smith. "Determination of Ochratoxin A by Monoclonal Antibody-Based Enzyme Immunoassay." Journal of AOAC INTERNATIONAL 71, no. 5 (1988): 961–64. http://dx.doi.org/10.1093/jaoac/71.5.961.

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Abstract A simple and rapid indirect enzyme-linked immunosorbent assay was developed for the quantitative determination of ochratoxin A in barley after the successful production of a high affinity, specific monoclonal antibody. A rapid sample cleanup was achieved by extracting ochratoxin A from barley with chloroform and partitioning the toxin into bicarbonate buffer; the buffer solution was then added directly to the assay plate and ochratoxin A content was assessed. Recoveries were greater than 85% and detection limits were 5 μg ochratoxin A/ kg barley.

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Yunianti, Faizah, Siswanto Darmadi, M. Y. Probohoesodo, and Budiono Budiono. "PERBANDINGAN PENENTUAN KADAR TIROKSIN ENZYME LINKED IMMUNOFLUORESCENT ASSAY (ELFA) DAN ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA)." INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 18, no. 1 (2016): 15. http://dx.doi.org/10.24293/ijcpml.v18i1.355.

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The determination of thyroxin (T4) is known as a good indicator to know the condition of thyroid function. In the hyperthyroid state, are shown clearly the increased levels of T4, while in the hypothyroid state their levels always decrease. The T4 levels change due to the physiological and pathological conditions on the ability of thyroxin binding globulin (TBG). The T4 measurement can be performed using an enzyme-linked fluorescent immunoassay (ELFA) or enzyme-linked immunosorbant assay (ELISA). Both ELFA and ELISA can detect T4 antigen. In these research fifty one randomized samples sera fro

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Azimi, Nooshin T., Fujiang Wen, Richard M. Lister, Dennis A. Chen, and Fred E. Lytle. "Enzyme-Linked Immunoassays Using Nanosecond Fluorometric Detection." Applied Spectroscopy 46, no. 6 (1992): 994–98. http://dx.doi.org/10.1366/0003702924124402.

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Nanosecond temporal resolution is combined with an enzyme-linked immunosorbent assay (ELISA) to improve the lower limit of detection for a plant virus, brome mosaic virus. The method uses alkaline phosphatase as the enzyme link and β-naphthyl phosphate as the substrate. Enzymatic activity produces the highly fluorescent tag β-naphthol. The 8.9-ns lifetime of the tag allows temporal discrimination against the assay blank, providing a 64× improvement in the detection limit as compared to a steady-state measurement, and a ∼4100× improvement over a standard ELISA method incorporating the chromogen

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Satterly, Neal G., Matthew A. Voorhees, Abbe D. Ames, and Randal J. Schoepp. "Comparison of MagPix Assays and Enzyme-Linked Immunosorbent Assay for Detection of Hemorrhagic Fever Viruses." Journal of Clinical Microbiology 55, no. 1 (2016): 68–78. http://dx.doi.org/10.1128/jcm.01693-16.

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ABSTRACT Viral hemorrhagic fevers, because of their high mortality rates, the lack of medical countermeasures, and their potential use as instruments of bioterrorism, pose a significant threat to the developed and the developing areas of the world. The key to preventing the spread of these diseases is early and accurate detection. For decades, the gold-standard immunoassay for hemorrhagic fever detection has been the enzyme-linked immunosorbent assay (ELISA); however, newer technologies are emerging with increased sensitivities. One such technology is the Luminex MagPix platform using xMAP mic

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Ganz, Tomas, Gordana Olbina, Domenico Girelli, Elizabeta Nemeth, and Mark Westerman. "Immunoassay for human serum hepcidin." Blood 112, no. 10 (2008): 4292–97. http://dx.doi.org/10.1182/blood-2008-02-139915.

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Abstract We developed and validated the first serum enzyme-linked immunosorbent assay for hepcidin, the principal iron-regulatory hormone that has been very difficult to measure. In healthy volunteers, the 5% to 95% range of hepcidin concentrations was 29 to 254 ng/mL in men (n = 65) and 17 to 286 ng/mL in women (n = 49), with median concentrations 112 versus 65 (P < .001). The lower limit of detection was 5 ng/mL. Serum hepcidin concentrations in 24 healthy subjects correlated well with their urinary hepcidin (r = 0.82). Serum hepcidin appropriately correlated with serum ferritin (r = 0.63

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Gruson, Damien, Thibault Lepoutre, and Françoise Smits. "Chromogranin-A Levels Measured with Automated Immunoassay." International Journal of Biological Markers 30, no. 1 (2015): 132–35. http://dx.doi.org/10.5301/jbm.5000096.

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Measurement of chromogranin-A (CgA) levels is relevant for the diagnosis of neuroendocrine neoplasms. The use of CgA testing for risk stratification of cardiovascular diseases is also increasing. The objective of our study was to determine the performances and reference values of a novel automated assay for CgA testing. The new method was compared with an enzyme-linked immunosorbent assay. Our results showed that the performances of the automated assay were satisfactory and that the agreement between the two methods was excellent. The automation of CgA testing also reduced the turnaround time

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Wu, John T. Y., Sally Dreger, Eva Y. W. Chow, Evelyn E. Bowlby, and Lester S. Y. Wong. "Developing an Automated Enzyme Immunoassay for High-Throughput Screening of Bovine Serum Antibodies to Neospora Caninum." JALA: Journal of the Association for Laboratory Automation 8, no. 1 (2003): 46–50. http://dx.doi.org/10.1016/s1535-5535-04-00241-2.

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An enzyme-linked immunosorbent assay (ELISA) for Neospora caninum antibodies was automated with a robotic workstation, the Beckman Coulter Biomek 2000, to screen 200 bovine sera. Comparing these results with manually run ELISA data, a 95.92% agreement (K = 0.9592) between the two assays was obtained. The automated assay was specific and sensitive with excellent positive and negative predictive values. The results were repeatable and reproducible. The automation flexibility was high and the operation complexity was minimal. High-throughput screening (HTS) for bovine antibodies to Neospora canin

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Yu, Xuezhi, Xiaoqi Tao, Jianzhong Shen, et al. "A one-step chemiluminescence immunoassay for 20 fluoroquinolone residues in fish and shrimp based on a single chain Fv–alkaline phosphatase fusion protein." Analytical Methods 7, no. 21 (2015): 9032–39. http://dx.doi.org/10.1039/c5ay01410g.

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Sharma, Rajesh, Pankaj Sharma, Gaush Talat, Praveen Gautam, Reba Chhabra, and Surinder Singh. "BRIEF OVERVIEW ON HEPATITIS C VIRUS IMMUNOASSAYS." International Journal of Research -GRANTHAALAYAH 4, no. 1 (2016): 178–84. http://dx.doi.org/10.29121/granthaalayah.v4.i1.2016.2862.

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The publication deals with a brief overview of Hepatitis C Virus (HCV) and donor blood screening for HCV by using conventional Rapid, Enzyme Linked Immunosorbent Assay (ELISA) and Chemiluminescence Immunoassay (CLIA) also. The advantages of various generation of HCV tests in terms of sensitivity, specificity and reduction in window period are discussed.

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NYQUIST-BATTIE, CYNTHIA, LAURA E. FRANK, DEANNA LUND, and DANIEL V. LIM. "Optimization of a Fluorescence Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Escherichia coli O157:H7 in Apple Juice." Journal of Food Protection 67, no. 12 (2004): 2756–59. http://dx.doi.org/10.4315/0362-028x-67.12.2756.

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Sandwich enzyme-linked immunosorbent assay, especially when coupled with biosensor technology, is a simple methodology that can rapidly screen juices for Escherichia coli O157:H7 contamination. However, sampling directly from apple juice and ciders has been postulated to reduce immunoassay sensitivity. In fluorescence sandwich enzyme-linked immunosorbent assays using commercially available polyclonal or monoclonal antibodies, sampling pasteurized apple juice spiked with E. coli O157:H7 compared to spiked phosphate-buffered saline shifted the range of detection. The spiked apple juice range of

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Topino, Simone, Laura Vincenzi, Ivano Mezzaroma, Emanuele Nicastri, Massimo Andreoni, and Maria C. Sirianni. "Correlation between Enzyme-Linked Immunosorbent Assay and Immunofluorescence Assay with Lytic Antigens for Detection of Antibodies to Human Herpesvirus 8." Clinical Diagnostic Laboratory Immunology 8, no. 1 (2001): 203–5. http://dx.doi.org/10.1128/cdli.8.1.203-205.2001.

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ABSTRACT The purpose of this study was to evaluate, in Kaposi's sarcoma patients, the correlation between antibody titers to the lytic antigens of human herpesvirus 8, as assessed by immunofluorescence assay, and values obtained by an enzyme immunoassay. The methods showed a stringent correlation, r = 0.625 (P< 0.001).

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MARAGOS, CHRIS M., and EUN-KYUNG KIM. "Detection of Zearalenone and Related Metabolites by Fluorescence Polarization Immunoassay." Journal of Food Protection 67, no. 5 (2004): 1039–43. http://dx.doi.org/10.4315/0362-028x-67.5.1039.

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Zearalenone is an estrogenic mycotoxin commonly found in grains throughout the world. A number of instrument- and antibody-based methods including enzyme-linked immunosorbent assays (ELISAs) have been developed to detect zearalenone (ZEN) and related toxins in commodities and foods. Although convenient, the commercial ELISAs for small molecules such as ZEN require a washing step to separate bound and unbound enzyme label before detection. In fluorescence polarization immunoassays, separation of bound and unbound label is not required, a property that reduces the time needed to perform the assa

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Cleary, John D., Stanley W. Chapman, Thomas C. Hardin, et al. "Amphotericin B Enzyme-Linked Immunoassay for Clinical Use: Comparison with Bioassay and HPLC." Annals of Pharmacotherapy 31, no. 1 (1997): 39–44. http://dx.doi.org/10.1177/106002809703100105.

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OBJECTIVE: To evaluate a new enzyme-linked immunosorbent assay (ELISA) for amphotericin B in serum samples. Results are compared with those obtained by HPLC and bioassay. DESIGN: Comparison of results obtained by ELISA, HPLC, and bioassay. METHODS: We developed a new ELISA using a polyclonal rabbit antibody to measure serum amphotericin B concentrations. Blinded samples of amphotericin B in concentrations of 0.15–78 μg/mL were prepared in human serum and assayed simultaneously by the ELISA, HPLC, and bioassay. The results of each assay were derived from standard curves and evaluated by using t

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Abe, Takaaki, Shunya Okamoto, Akinobu Taniguchi, et al. "A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay." Analytical Methods 12, no. 40 (2020): 4858–66. http://dx.doi.org/10.1039/d0ay01459a.

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In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding.

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Gall, D., K. Nielsen, W. Yu, and P. Smith. "Rapid, Field-Adapted Indirect Enzyme-Linked Immunosorbent Assay for Detection of Antibodies in Bovine Whole Blood and Serum to Brucella abortus." Clinical and Vaccine Immunology 13, no. 4 (2006): 501–6. http://dx.doi.org/10.1128/cvi.13.4.501-506.2006.

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ABSTRACT A simple, rapid, field-adapted indirect enzyme-linked immunoassay (FldELISA) for the detection of antibodies to Brucella abortus in whole blood and serum has been developed. This assay detects antibodies to B. abortus in approximately 15 min or less. Over a 3-month period, this assay has consistently identified immunized and nonimmunized animals, while the percent coefficient of variation for each immunized animal has been less than 20%. As with any indirect enzyme-linked immunoassay, quality control can be established and maintained. Using defined positive and negative sera, the sens

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Loginov, S. I. "Analysis of the effectiveness of the use of enzyme-linked immunosorbent assay for the diagnosis of leukemia in cattle during health-improving activities." Bulletin of NSAU (Novosibirsk State Agrarian University), no. 4 (December 31, 2020): 95–102. http://dx.doi.org/10.31677/2072-6724-2020-57-4-95-102.

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The aim of the work is to analyze the efficiency of using enzyme-linked immunosorbent assay in the diagnosis of cattle leukemia when carrying out health-improving measures in livestock enterprises that are unfavorable for this disease. Indicators of infection of cattle with leukemia virus on 6 farms of agricultural enterprises of the Tomsk region are presented. For serological diagnostics, the immunodiffusion reaction and enzyme immunoassay were used. It has been established that while carrying out health-improving measures in enterprises unfavorable for leukemia in cattle, the use of enzyme-l

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Loginov, S. I. "Analysis of the effectiveness of the use of enzyme-linked immunosorbent assay for the diagnosis of leukemia in cattle during health-improving activities." Bulletin of NSAU (Novosibirsk State Agrarian University), no. 4 (December 31, 2020): 95–102. http://dx.doi.org/10.31677/2072-6724-2020-57-4-95-102.

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The aim of the work is to analyze the efficiency of using enzyme-linked immunosorbent assay in the diagnosis of cattle leukemia when carrying out health-improving measures in livestock enterprises that are unfavorable for this disease. Indicators of infection of cattle with leukemia virus on 6 farms of agricultural enterprises of the Tomsk region are presented. For serological diagnostics, the immunodiffusion reaction and enzyme immunoassay were used. It has been established that while carrying out health-improving measures in enterprises unfavorable for leukemia in cattle, the use of enzyme-l

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Oelemann, Walter M. R., Maria Da Glória M. Teixeira, Giovani C. Veríssimo Da Costa, et al. "Evaluation of Three Commercial Enzyme-Linked Immunosorbent Assays for Diagnosis of Chagas’ Disease." Journal of Clinical Microbiology 36, no. 9 (1998): 2423–27. http://dx.doi.org/10.1128/jcm.36.9.2423-2427.1998.

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Chagas’ disease is a common cause of morbidity in Latin American countries. In Brazil, naturally occurring transmission of its etiologic agent, Trypanosoma cruzi, has been almost completely abolished through effective control programs aimed at the triatomid insect vector. Thus, transfusion of blood from infected donors has become the major route for contracting Chagas’ disease due to the socioeconomically motivated migration of residents from areas where the disease is endemic to the larger urban centers. Therefore, the employment of screening tests is mandatory for all blood banks throughout

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Peuravuori, H., and T. Korpela. "Pyrophosphatase-based enzyme-linked immunosorbent assay of total IgE in serum." Clinical Chemistry 39, no. 5 (1993): 846–51. http://dx.doi.org/10.1093/clinchem/39.5.846.

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Abstract Human IgE was purified to near homogeneity by a two-step procedure consisting of immunoaffinity chromatography and high-performance liquid chromatography. Mouse hybridoma cell lines secreting antibodies against IgE were generated. Monoclonal and polyclonal antibodies were used to develop a "sandwich"-type ELISA for determining total IgE in human serum. Inorganic pyrophosphatase (EC 3.6.1.1), an enzyme having a high turnover number, was used as the label. The mean analytical recovery of our ELISA was 95.2% and the results showed good linear correlation with an established RIA of IgE. W

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Vo-Dinh, T., G. D. Griffin, and K. R. Ambrose. "A Portable Fiberoptic Monitor for Fluorimetric Bioassays." Applied Spectroscopy 40, no. 5 (1986): 696–700. http://dx.doi.org/10.1366/0003702864508575.

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A simple and portable fiberoptic instrument for fluorimetric bioassays is described. The instrument is designed to be readily adaptable to commercially available biotesting wells on microplates. The microwells can also be used as interchangeable sensor heads. The utility of the fiberoptic biosensor is illustrated with a model enzyme-linked immunosorbent assay (ELISA) used to measure varying amounts of rabbit immunoglobulin G from 10 pg to 1 μg. The detection method utilizes an enzyme-amplified immunoassay of 4-methylumbelliferone phosphate as the enzyme substrate. The sensitivity of this assay

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Deng, Dehua, Yuanqiang Hao, Jiajia Xue, Xiuhua Liu, Xinyue Xu, and Lin Liu. "A Colorimetric Enzyme-Linked Immunosorbent Assay with CuO Nanoparticles as Signal Labels Based on the Growth of Gold Nanoparticles In Situ." Nanomaterials 9, no. 1 (2018): 4. http://dx.doi.org/10.3390/nano9010004.

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A colorimetric immunoassay has been reported for prostate-specific antigen (PSA) detection with CuO nanoparticles (CuO NPs) as signal labels. The method is based on Cu2+-catalyzed oxidation of ascorbic acid (AA) by O2 to depress the formation of colored gold nanoparticles (AuNPs). Specifically, HAuCl4 can be reduced by AA to produce AuNPs in situ. In the presence of target, CuO NPs-labeled antibodies were captured via the sandwich-type immunoreaction. After dissolving CuO nanoparticles with acid, the released Cu2+ catalyzed the oxidation of AA by O2, thus depressing the generation of AuNPs. To

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Bury, J., and M. Rosseneu. "Quantification of human serum apolipoprotein AI by enzyme immunoassay." Clinical Chemistry 31, no. 2 (1985): 247–51. http://dx.doi.org/10.1093/clinchem/31.2.247.

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Abstract We developed a quantitative assay for apolipoprotein AI (apo AI) in human serum, using a "sandwich"-type enzyme-linked immunosorbent assay. Diluted serum samples were pipetted into the wells of polystyrene microtiter plates that had been previously coated with purified rabbit anti-human apo AI antibodies. After incubation for 2 h and washing, antibodies conjugated to horseradish peroxidase (EC 1.11.1.7) were added and incubated for 2 h; after further washing, the bound enzyme was assayed by oxidation of o-phenylenediamine. Assay conditions were optimized for the incubation time and th

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Kvas, Maia, Alyne G. Teixeira, Beatrice Chiang, and John P. Frampton. "Aqueous two-phase system antibody confinement enables cost-effective analysis of protein analytes by sandwich enzyme-linked immunosorbent assay with minimal optical crosstalk." Analyst 145, no. 16 (2020): 5458–65. http://dx.doi.org/10.1039/d0an00699h.

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Chen, Han Yu, and Hui Sheng Zhuang. "Development of an Analytical Protocol for the Determination of Polybrominated Biphenyls in Water by Enzyme-Linked Immunosorbent Assay." Advanced Materials Research 347-353 (October 2011): 1537–41. http://dx.doi.org/10.4028/www.scientific.net/amr.347-353.1537.

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A novel immunoassay has been developed for the quantitative determination of polybrominated biphenyls (PBBs) using indirect competitive format. A new method was developed to synthesize PBBs congener (PCB15) hapten and its artificial immunogen, then the polyclonal antibodies. The assay was optimized concerning the coating conjugate and antibody concentration, incubation time and temperature, the tolerance to organic solvents and so on. Under optimized conditions, PBB15 can be determined in the concentration range of 0.01-100 μg L-1 with a detection limit of 0.02 μg L-1. The cross-reactivities o

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