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Journal articles on the topic 'Immunochromatographic test system'

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1

Shao, Lei, Longyu Zhang, Shilin Li, and Pengyuan Zhang. "Design and Quantitative Analysis of Cancer Detection System Based on Fluorescence Immune Analysis." Journal of Healthcare Engineering 2019 (December 24, 2019): 1–9. http://dx.doi.org/10.1155/2019/1672940.

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Human blood is an important medical detection index. With the development in clinical medical detection instruments and detection technology, the requirements for detection accuracy and efficiency have been gradually improved. Fluorescent immunochromatography is a new detection technique. It has the characteristics of high efficiency, convenience, no pollution, and wide detection range. Human blood can be detected quickly using fluorescent immunochromatography. At present, it has received great attention from the field of clinical testing. In this paper, a set of fluorescent immunochromatograp
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Zherdev, A. V., E. A. Zvereva, N. A. Taranova, I. V. Safenkova, N. L. Vostrikova, and B. B. Dzantiev. "Immunochromatographic food control tools: New developments and practical prospects." Theory and practice of meat processing 9, no. 4 (2025): 280–95. https://doi.org/10.21323/2414-438x-2024-9-4-280-295.

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In the modern food production technologies, the tools and means of simple and rapid testing raw materials, intermediate products and the final ready-to-consume food products are in high demand. This monitoring allows determining the content of toxic and pathogenic contaminants and confirms the compliance of the objects being tested with the established regulatory requirements. Mobile tests tools and means (so called test systems) provide the opportunity of wide range monitoring without involving the specialized laboratories and highly qualified specialists. Thus, test systems for detection of
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Galeeva, A. G., Sh M. Nasyrov, G. S. Kashevarov, et al. "Immunochromatographic test-system for express diagnostics of rabies." Agrarian science, no. 9 (September 22, 2023): 32–37. http://dx.doi.org/10.32634/0869-8155-2023-374-9-32-37.

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Relevance. Rabies is included in the group of transmissible viral zooanthroponoses that globally affect public health and the public economy. In Russian Federation, according to the State Standard 26075-2013, the method of virus isolation is a priority for the diagnosis of rabies, followed by detection by the method of fluorescent antibodies (MFA), however, the call of the World Health Organization to abandon research using animals makes it relevant to further develop and improve diagnostic methods in vitro.The purpose of the study. Designing a test system based on immunochromatographic analys
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Nikitina, A. V., S. G. Mardanly, and V. V. Pomazanov. "COMPARATIVE EVALUATION OF THE IMMUNOCHROMATOGRAPHIC TEST FOR DETECTION OF HEMOGLOBIN." Russian Clinical Laboratory Diagnostics 65, no. 7 (2020): 439–42. http://dx.doi.org/10.18821/0869-2084-2020-65-7-439-442.

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The evaluating results of the diagnostic characteristics of an immunochromatographic test for the detection of fecal occult blood (hemoglobin) were presents in the article. The test was approved samples without hemoglobin and model samples containing of standardized preparation of hemoglobin in various concentrations in comparison with two immunochromatographic test systems. The developed test system identified hemoglobin in concentrations higher 50 ng/ml in 99,2% cases, the specificity of the analysis was 98,1%. The obtained levels of sensitivity and specificity were higher the similar parame
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Li, Yifan, Zhenfeng Li, Baozhu Jia, et al. "Detection of AFB1 by Immunochromatographic Test Strips Based on Double-Probe Signal Amplification with Nanobody and Biotin–Streptavidin System." Foods 13, no. 21 (2024): 3396. http://dx.doi.org/10.3390/foods13213396.

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Aflatoxin B1 (AFB1) is highly toxic and difficult to prevent. It is mainly produced by fungi and exists in plants and animals and is classified by the World Health Organization as a class I carcinogen, posing a serious threat to human and animal health. Therefore, it is important to establish an efficient, sensitive, and on-site detection method for AFB1 to protect human health. The immunochromatographic test strip method is simple, sensitive, and can achieve real-time detection. However, traditional immunochromatographic test strips have low sensitivity due to their relatively weak optical pr
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Nikitina, Anna Viktorovna, Y. A. Akinshina, N. E. Nishchakova, E. A. Amelina, and S. G. Mardanly. "IMMUNOCHROMATOGRAPHIC TEST FOR DETECTION OF FECAL OCCULT BLOOD." Russian Clinical Laboratory Diagnostics 64, no. 9 (2019): 536–40. http://dx.doi.org/10.18821/0869-2084-2019-64-9-536-540.

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The developing and evaluating results of the diagnostic characteristics of an immunochromatographic test for the detection of fecal occult blood (hemoglobin) were presents in the article. The test was approved samples without hemoglobin and model samples containing of standardized preparation of hemoglobin in various concentrations. The developed test system identified hemoglobin in concentrations higher 5 μg/g feces in 97.6% cases, the specificity of the analysis was 100%. The immunochromatographic FOB-test can be used for rapid analysis in the primary screening of colorectal cancer.
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Sotnikov, Dmitriy V., Lyubov V. Barshevskaya, Anatoly V. Zherdev, et al. "Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots." Applied Sciences 10, no. 3 (2020): 738. http://dx.doi.org/10.3390/app10030738.

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In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: Gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochromatographic serodiagnostics systems that use only one marker. The approbation of the test system was conducted on samples of cattle sera with low, but diagnostically significant titers of specific antibodies. We show that when two conjugates are used, the intensity of the detectable signal increases by 2–3 times compared with the test system using the QD conjug
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Zhumalin, Aibek, Zhanbolat Suranshiyev, Orken Akibekov, and Gulbadan Otepova. "DEVELOPMENT OF A TEST SYSTEM FOR RAPID DIAGNOSIS OF TRICHINELLOSIS IN ANIMALS." 3i intellect idea innovation - интеллект идея инновация 1 (2025): 43–51. https://doi.org/10.52269//22266070_2025_1_43.

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Trichinosis is a parasitic disease caused by nematodes of the Trichinella genus, posing significant concerns for both veterinary and medical fields. This disease is widespread in many countries, including Kazakhstan. Trichinosis primarily spreads through domestic and wild animals, and humans can contract it through raw or undercooked meat. In recent years, the spread of trichinosis in Asia, Europe, and other regions has shown an increasing trend. In Kazakhstan, the disease is mainly transmitted through dog, badger, and wild boar meat. Traditional diagnostic methods, such as trichinoscopy and s
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Smirnova, D. N., N. V. Bogacheva, S. B. Petrov та N. A. Tuneva. "Comparative assessment of the results of detection of СagА-positive strains of Helicobacter pylori by molecular-genetic and immunochromatographic methods in different biological materials". Russian Clinical Laboratory Diagnostics 67, № 1 (2022): 48–52. http://dx.doi.org/10.51620/0869-2084-2022-67-1-48-52.

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A comparative analysis of the detection of CagA-positive strains of H. pylori by immunochromatographic and molecular genetic methods was carried out. We used H. pylori strains isolated from individuals with diseases of the gastrointestinal tract. The immunochromatographic method was implemented using a developed experimental model of an immunochromatographic test system for detecting the H. pylori CagA protein in various biological materials. Determination of the pathogenicity gene cagA of H. pylori was carried out using the «Helikopol SA» test system («Litekh», Russia). The assessment of the
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Petrakova, A. V., A. E. Urusov, M. V. Voznyak, A. V. Zherdev, and B. B. Dzantiev. "Immunochromatographic test system for the detection of T-2 toxin." Applied Biochemistry and Microbiology 51, no. 6 (2015): 688–94. http://dx.doi.org/10.1134/s0003683815060113.

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Ragozina, M. Yu, N. S. Komova, K. V. Serebrennikova, A. V. Zherdev, and B. B. Dzantiev. "LATERAL FLOW TECHNIQUE FOR THE DETERMINATION OF DIBUTYL PHTHALATE." BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES 1, no. 2022-20 (2022): 139–40. http://dx.doi.org/10.37747/2312-640x-2022-20-139-140.

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An immunochromatographic method for the rapid determination of dibutyl phthalate has been developed. For this purpose, the competitive immunochemical interaction of monoclonal antibodies labeled with gold nanoparticles with a hapten-protein conjugate and a free analyte in various media was characterized. An immunochromatographic test system has been tested to detect and evaluate the content of dibutyl phthalate in natural waters.
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Wang, Jing-Yun, Ming-Hui Chen, Zhi-Chao Sheng, Dao-Feng Liu, Song-Song Wu, and Wei-Hua Lai. "Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk." RSC Advances 5, no. 76 (2015): 62300–62305. http://dx.doi.org/10.1039/c5ra13279g.

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Gorshkova, R. M., D. A. Slobodova, N. P. Novoselov, and P. P. Gladyshev. "Multiplexed Immunochromatographic Test-System for Rapid Diagnosis of Acute Heart Failure." Fibre Chemistry 52, no. 4 (2020): 247–50. http://dx.doi.org/10.1007/s10692-021-10189-y.

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Spada, Eva, Daniela Proverbio, Luciana Baggiani, Giada Bagnagatti De Giorgi, Roberta Perego, and Elisabetta Ferro. "Evaluation of an immunochromatographic test for feline AB system blood typing." Journal of Veterinary Emergency and Critical Care 26, no. 1 (2015): 137–41. http://dx.doi.org/10.1111/vec.12360.

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Wicaksono, Wiyogo Prio, and Arnis Prameswari Putri. "Electrochemical Immunochromatographic Strip Test for Melamine Biosensor in Milk Products Using Silver Nanoparticles as Probe." Applied Mechanics and Materials 490-491 (January 2014): 1624–28. http://dx.doi.org/10.4028/www.scientific.net/amm.490-491.1624.

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A quantitative and sensitive immunochromatographic strip test is developed to detect of melamine in milk products. The strip test based on antigen-antibody (melamine-anti melamine) complex reaction. As label to antibody is used silver nanoparticles to form antibody-silver nanoparticles (AgNP-Ab). The strip test consists of sample pad, conjugated pad (AgNP-Ab), nitrocelloluse membrane, test zone (Ab), and absorbant pad. When melamine is found in the sample, then antibody-melamine-antibody-silver nanoparticles complex was made. Silver nanoparticles have good electrochemical properties. Electroch
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Barshevskaya, Lyubov V., Dmitriy V. Sotnikov, Anatoly V. Zherdev, et al. "Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia." Biosensors 9, no. 4 (2019): 115. http://dx.doi.org/10.3390/bios9040115.

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An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A/G and three analytical zones with immobilized pathogen antigens: Brucella abortus lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of Mycobacterium bovis, and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The di
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Andreeva, I. P., E. A. Yakovleva, V. G. Grigorenko, and A. P. Osipov. "The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers." Moscow University Chemistry Bulletin 74, no. 6 (2019): 315–21. http://dx.doi.org/10.3103/s002713141906004x.

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Byzova, N. A., A. V. Zherdev, P. G. Sveshnikov, E. G. Sadykhov, and B. B. Dzantiev. "Development of an immunochromatographic test system for the detection of Helicobacter pylori antigens." Applied Biochemistry and Microbiology 51, no. 5 (2015): 608–17. http://dx.doi.org/10.1134/s000368381505004x.

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Nash, Michael A., John M. Hoffman, Dean Y. Stevens, Allan S. Hoffman, Patrick S. Stayton, and Paul Yager. "Laboratory-scale protein striping system for patterning biomolecules onto paper-based immunochromatographic test strips." Lab on a Chip 10, no. 17 (2010): 2279. http://dx.doi.org/10.1039/c004991c.

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Hendrickson, Olga D., Elena A. Zvereva, Demid S. Popravko, Anatoly V. Zherdev, Chuanlai Xu, and Boris B. Dzantiev. "An immunochromatographic test system for the determination of lincomycin in foodstuffs of animal origin." Journal of Chromatography B 1141 (March 2020): 122014. http://dx.doi.org/10.1016/j.jchromb.2020.122014.

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Byzova, N. A., L. N. Lukhverchik, A. V. Zherdev, N. V. Piven, A. I. Burakovskii, and B. B. Dzantiev. "Development of an immunochromatographic test system for the detection of human epidermal growth factor." Applied Biochemistry and Microbiology 49, no. 6 (2013): 606–12. http://dx.doi.org/10.1134/s0003683813060033.

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Yen, Sheng-Lun, Minh Hoang, Wei-Chen Wang, et al. "DETECTION OF CANINE PARVOVIRUS TYPE 2 BY A COMMERCIALLY AVAILABLE IN-HOUSE PCR." Taiwan Veterinary Journal 46, no. 01 (2020): 37–43. http://dx.doi.org/10.1142/s1682648519500069.

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Canine parvovirus type 2 (CPV-2) is an important pathogen that causes anorexia, vomiting, acute gastroenteritis, and bloody diarrhea. CPV-2 was divided into three variants (2a–2c) based on residue 426 of the VP2 protein. Recently, a novel Asian CPV-2c isolate was more prevalent in the Asian continent. The diagnostic tools of CPV-2 are characterized into traditional (such as immunochromatography test) and molecular methods based on their pathogen detection mechanisms. However, the low sensitivity of the immunochromatographic tests is supported by their simplicity and rapid ability to provide re
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Sebastian, Anju, and Archana S. "Diagnostic Utility of a Rapid Immunochromatographic Test for Procalcitonin in Paediatric Infections." International Journal of Health Sciences and Research 13, no. 6 (2023): 1–5. http://dx.doi.org/10.52403/ijhsr.20230601.

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Procalcitonin (PCT) is an inflammatory marker that has been used as an indicator of severe bacterial infection. A rapid immunochromatographic test was used in this study, which can be used for on-site detection of PCT. A prospective study was done in the Department of Microbiology of a tertiary care hospital from March 2021 to February 2022. 227 patients were included in the study. Respiratory tract infection was the commonest infection (40.8%) in children followed by central nervous system, gastrointestinal tract and urinary tract infections. Out of 227 patients, 23patients had positive blood
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Lin, Kai-Wen, and Yu-Chi Chang. "Embedded Immunodetection System for Fecal Occult Blood." Biosensors 11, no. 4 (2021): 106. http://dx.doi.org/10.3390/bios11040106.

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In this paper, a rapid test system with high sensitivity, linearity, and stability is presented for fecal occult blood (FOB) detection. The coloration results of the immune response are used as the basis for the determination of the detection target in combination with an immunochromatographic strip. The rapid test system can be used to detect and calculate the concentration of the sample, so detection of the immune coloration response is more accurate in a quantitative analysis. The system is composed of both hardware and software. The programs used for the analysis and programmed by Python i
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Jangulova, A. N., Zh Zh Akanova, and A. K. Bulashev. "Using of immunochromatographic analysis to determine antibiotics in milk." HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY: Veterinary sciences, no. 2(006) (June 28, 2024): 36–44. http://dx.doi.org/10.51452/kazatuvc.2024.2(006).1713.

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Background and Aim. Failure to comply with the rules for the use of antibiotics and/or the tim-ing of milk production from cows subjected to antibiotic therapy leads to the entry of residual amounts of drugs into the human body with dairy products, causing various pathologies. For practice, simple but sufficiently sensitive and specific rapid tests are needed to determine the safety of milk. Such tests can be developed based on immunochromatographic analysis (ICA). Materials and Methods. Colloidal gold (CG) particles were prepared according to the method of Wang et.al. (2014) and examined usin
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Abbas, Muhammad, Nasima Iqbal, Rahman Ullah, Waheed Ur Rahman, and Habib Ullah. "Immunochromatographic Detection of SARS CoV-2 Antigen In Comparison With the Polymerase Chain Reaction for Laboratory Diagnosis of Covid-19 in Karachi, Pakistan." Journal of Rehman Medical Institute 9, no. 4 (2024): 03–07. http://dx.doi.org/10.52442/jrmi.v9i4.569.

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Introduction: The Covid-19 pandemic raised the question of which laboratory test was relevant for a rapid and accurate diagnosis of the disease. The current two methods for the detection of SARS CoV-2 are the ICT (Immunochromatographic Technique) and the PCR (Polymerase Chain Reaction) tests; therefore comparative analysis of test performance has to be done. Objective: To perform a comparative analysis of two different techniques (Immunochromatographic technique for Antigen and Polymerase Chain Reaction for RNA) which are implicated for the detection of SARS CoV-2. Materials & Methods: The
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Razo, S., V. G. Panferov, I. V. Safenkova, et al. "Development of an immunochromatographic test system with nanozyme amplification for detecting the phytopathogen Erwinia amylovora." Achievements of Science and Technology in Agro-Industrial Complex 36, no. 1 (2022): 34–39. http://dx.doi.org/10.53859/02352451_2022_36_1_34.

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Jin, Shiqun, Lihao Bai, Guo Xia, et al. "A Smartphone-Based Detection Method of Colloidal Gold Immunochromatographic Strip." Photonics 8, no. 12 (2021): 576. http://dx.doi.org/10.3390/photonics8120576.

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The outbreak of the new coronavirus (SARS-CoV-2) infection has become a global public health crisis. Antigen detection strips (colloidal gold) can be widely used in novel coronavirus clinical screening and can even be extended to home self-testing, which provides a practical and effective way for people to obtain health status information away from the crowd. In this paper, a colloidal gold detection system without complex devices is proposed, which is based on smartphone usage along with a mobile-phone software embedded with normalization algorithms and a special designed background paper. Th
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Zvereva, Elena A., Nadezhda A. Byzova, Olga D. Hendrickson, et al. "Immunochromatographic Detection of Myoglobin as a Specific Biomarker of Porcine Muscle Tissues in Meat Products." Applied Sciences 10, no. 21 (2020): 7437. http://dx.doi.org/10.3390/app10217437.

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An immunochromatographic detection of myoglobin (MG) as a specific marker of porcine muscle tissue has been developed. The method is based on the sandwich lateral flow immunoassay (LFIA) with gold nanoparticles (AuNPs) as a label. The developed test system determines MG with a detection limit of 5 ng mL−1 within 15 min. A specific determination of porcine MG and no cross-reactivity with MG from other tested mammals and bird species was demonstrated. The test system is able to detect pork additives, as low as 0.01% (w/w), in minced beef. A technique of MG extraction from muscle tissue has been
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Zvereva, E. A., D. V. Sotnikov, K. A. Belichenko, et al. "Development of Immunochromatographic Test System for Detection of Antibiotic Clinafloxacin and Its Application for Honey Control." Applied Biochemistry and Microbiology 57, no. 6 (2021): 778–85. http://dx.doi.org/10.1134/s0003683821060144.

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Alekseeva, S. A., I. V. Kasina, and T. I. Nemirovskaya. "Comparative analysis of the results of live anthrax vaccine identification by immunofluorescence and immunochromatography." Biological Products. Prevention, Diagnosis, Treatment 24, no. 3 (2024): 348–56. http://dx.doi.org/10.30895/2221-996x-2024-24-3-348-356.

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INTRODUCTION. The quality evaluation of live anthrax vaccines will benefit from the implementation of alternative testing methods that are capable of specific identification of the Bacillus anthracis spore antigen using the appropriate diagnostic products that are authorised in the Russian Federation for the detection of B. anthracis spores.AIM. This study aimed to investigate the applicability of immunofluorescence analysis to the identification of live anthrax vaccines and compare this method with immunochromatography.MATERIALS AND METHODS. The study used commercial batches of a live anthrax
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Tao, Sirui, Yu Duan, Yinhe Zha, et al. "Development and Evaluation of an Immunochromatographic Strip and a Magnetic Chemiluminescence Immunoassay for Detection of Porcine Circovirus Type 2 Antigen." Veterinary Sciences 12, no. 1 (2025): 40. https://doi.org/10.3390/vetsci12010040.

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Porcine circovirus type 2 (PCV2) is the main and primary causative agent of Postweaning Multisystemic Wasting Syndrome (PMWS). To date, immunoperoxidase monolayer assay (IPMA), indirect immunofluorescent assay (IFA), and enzyme linked immunosorbent assay (ELISA) are the most commonly diagnostic methods for detecting PCV2 antigens. However, these methods require specialized equipment and technical expertise and are suitable for laboratory use only. This study aims to develop an immunochromatographic strip and a magnetic chemiluminescence immunoassay for the detection of PCV2 antigens. The recom
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Zvereva, Elena A., Olga D. Hendrickson, Boris B. Dzantiev, and Anatoly V. Zherdev. "Lateral Flow Test System to Control Total Content of Muscle Tissues in Raw Meat Products." Sensors 22, no. 24 (2022): 9724. http://dx.doi.org/10.3390/s22249724.

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Assessment of the composition of meat-containing products is the task in demand due to their frequent deviations from declared recipes. The paper presents the developed test system for immunochromatographic determination of total meat content. The assay is based on the simultaneous use of monoclonal antibodies, which specifically interacts with mammalian skeletal troponin I, and polyclonal antibodies, which specifically detect bird immunoglobulin Y. To integrate the detection of both types of meat by the same test strip, the antibodies are mixed in the analytical zone of the test strip and in
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Hendrickson, Olga D., Elena A. Zvereva, Anatoly V. Zherdev, Tzonka Godjevargova, Chuanlai Xu, and Boris B. Dzantiev. "Development of a double immunochromatographic test system for simultaneous determination of lincomycin and tylosin antibiotics in foodstuffs." Food Chemistry 318 (July 2020): 126510. http://dx.doi.org/10.1016/j.foodchem.2020.126510.

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Hendrickson, Olga D., Elena A. Zvereva, Olga N. Solopova, et al. "Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood." Micromachines 13, no. 9 (2022): 1506. http://dx.doi.org/10.3390/mi13091506.

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In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelk
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Hendrickson, Olga D., Nadezhda A. Byzova, Irina V. Safenkova, Vasily G. Panferov, Boris B. Dzantiev, and Anatoly V. Zherdev. "Sensitive Immunochromatographic Determination of Salmonella typhimurium in Food Products Using Au@Pt Nanozyme." Nanomaterials 13, no. 23 (2023): 3074. http://dx.doi.org/10.3390/nano13233074.

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In this study, we developed a sensitive immunochromatographic analysis (ICA) of the Salmonella typhimurium bacterial pathogen contaminating food products and causing foodborne illness. The ICA of S. typhimurium was performed using Au@Pt nanozyme as a label ensuring both colorimetric detection and catalytic amplification of the analytical signal due to nanozyme peroxidase-mimic properties. The enhanced ICA enabled the detection of S. typhimurium cells with the visual limit of detection (LOD) of 2 × 102 CFU/mL, which outperformed the LOD in the ICA with traditional gold nanoparticles by two orde
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Singov, Evgeny K., Оleg S. Morenkov, Sergey V. Sipin, and Veronika V. Vrublevskaya. "Development and validation of an immunochromatographic test system for the determination of microcystins in water and tissues of freshwater fish." Toxicological Review 32, no. 3 (2024): 187–94. http://dx.doi.org/10.47470/0869-7922-2024-32-3-187-194.

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Introduction. Certain genera of cyanobacteria produce hepatotoxins associated with the group of microcystins (MCs). During periods of water blooms, cases of poisoning and death of fish, birds, wild and domestic animals, as well as humans, associated with the consumption of water, blue-green algae or other aquatic organisms contaminated with MC, have been recorded. Timely detection of MC in water reservoirs, in drinking water and in hydrobionts is necessary for carrying out preventive measures to prevent poisoning of people and animals with microcystins. Results. An immunochromatographic test s
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Omer, Duria Abdelraheim Mohammed Ahmed, Widad Alsheikh, and Ahmed Yousif Adam. "The Reliability of Immunochromatographic Test in Tuberculous Children Diagnosed by Scoring System at Mohamed Elamin Hamid Hospital – Sudan." IOSR Journal of Nursing and Health Science 06, no. 02 (2017): 35–40. http://dx.doi.org/10.9790/1959-0602053540.

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Hendrickson, OD, EA Zvereva, IA Shanin, AV Zherdev, and BB Dzantiev. "Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products." Journal of the Science of Food and Agriculture 99, no. 8 (2019): 3834–42. http://dx.doi.org/10.1002/jsfa.9605.

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Suksomboon, Phawiya, Pornanan Kueakhai, and Narin Changklungmoa. "Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection." Tropical Medicine and Infectious Disease 8, no. 3 (2023): 164. http://dx.doi.org/10.3390/tropicalmed8030164.

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Fasciolosis is a zoonotic disease caused by Fasciola gigantica or F. hepatica infections, which are frequently occurring parasites in animals and humans. The present gold-standard diagnostic technique involves finding parasite eggs through microscopy. However, this method is also restricted due to low specificity and low sensitivity. An alternative to coprological diagnosis is the immunochromatographic strip (ICS) test, which is rapid, simple, convenient, and cost-effective, with high sensitivity and high specificity. Cathepsin L1H (CathL1H) is a cysteine protease secreted by F. gigantica, whi
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Ozkan, Haydar, and Osman Semih Kayhan. "A Novel Automatic Rapid Diagnostic Test Reader Platform." Computational and Mathematical Methods in Medicine 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/7498217.

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A novel automatic Rapid Diagnostic Test (RDT) reader platform is designed to analyze and diagnose target disease by using existing consumer cameras of a laptop-computer or a tablet. The RDT reader is useable with numerous lateral immunochromatographic assays and similar biomedical tests. The system has two different components, which are 3D-printed, low-cost, tiny, and compact stand and a decision program named RDT-AutoReader 2.0. The program takes the image of RDT, crops the region of interest (ROI), and extracts the features from the control end test lines to classify the results as invalid,
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Petrova, O. V., D. K. Tverdokhlebova, O. I. Murygina, E. V. Smeltsova, and D. M. Nikulina. "Diagnostic characteristics of serological tests for the detection of SARS-CoV-2 cases." Russian Clinical Laboratory Diagnostics 66, no. 4 (2021): 210–12. http://dx.doi.org/10.51620/0869-2084-2021-66-4-210-212.

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To study the diagnostic characteristics of test systems for detecting antibodies to SARS-Cov-2. We studied the diagnostic characteristics of two test systems for detecting antibodies to SARS-Cov-2, registered in the Russian Federation. The first test system is a kit for detecting total antibodies to SARS-Cov-2 using immunochemiluminescence analysis on the «Cobas e 411» analyzer («Roche Diagnostics», Germany). The second test system is a kit for detecting IgM and IgG to SARS-Cov-2 («Core Technology Co., Ltd», China) by immunochromatographic analysis. The biological material for the study was bl
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Lacorn, Markus, Katharina Scherf, Steffen Uhlig, et al. "Determination of Gluten in Processed and Nonprocessed Corn Products by Qualitative R5 Immunochromatographic Dipstick: Collaborative Study, First Action 2015.16." Journal of AOAC INTERNATIONAL 99, no. 3 (2016): 730–37. http://dx.doi.org/10.5740/jaoacint.16-0017.

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Abstract In September 2013, the AACC International (AACI) Protein Technical Committee (now Protein and Enzymes Technical Committee) initiated a collaborative study of a method for the qualitative analysis of intact gluten in processed and nonprocessed corn products, using an R5 immunochromatographic dipstick system. It was validated to demonstrate that potential gluten-free products contain gluten lower than the Codex threshold of 20 mg/kg gluten. The results of the collaborative test with 18 participants confirmed that the method is suitable to detect gluten contaminations that are clearly lo
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Ben Aissa, Alejandra, Barbara Araújo, Esther Julián, Maria Valnice Boldrin Zanoni, and María Isabel Pividori. "Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays." Sensors 21, no. 18 (2021): 5992. http://dx.doi.org/10.3390/s21185992.

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This work addresses a method that combines immunomagnetic separation (IMS) and paper-based nucleic acid immunochromatographic assay for the sensitive detection of Mycolicibacterium fortuitum (basonym Mycobacterium fortuitum) In particular, the preconcentration of the bacteria was achieved by using magnetic particles modified with an antibody specific towards mycobacteria. Following the IMS, the bacteria were lysed, and the genome was amplified by double-tagging PCR, using a set of primers specific for the 16S rRNA gene for Mycobacterium. During the amplification, the amplicons were labeled wit
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Lu, Yi-Kuan, Di Xu, Wen-Yue Liu, Jing Xie, and Ying Lu. "A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green." Biosensors 12, no. 7 (2022): 456. http://dx.doi.org/10.3390/bios12070456.

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In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T1 line, blue T2 line and purple C line), a visual identification of TMS (0.2 μg/mL) and MG (0.5 μg/mL) was quickly achieved on site, which improved the accuracy of naked eye observations. The LODs of TMS in aquaculture water, fish and shrimp were 11.0, 29.6 and 61.4 ng/mL, respectively. MG LODs were 47.0 ng/mL (aquacultu
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Wu, Xiaoxia, Xiaofeng Tian, Lihua Xu, Jiutong Li, Xinxia Li, and Yuwen Wang. "Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay." BioMed Research International 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/8649314.

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Harsh demanding has been exposed on the concentration of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. In this study, we developed a new method based on background fluorescence quenching immunochromatographic assay (bFQICA) to detect AFM1 and CAP in milk. The detection limit for AFM1 was 0.0009 ng/mL, while that for the CAP was 0.0008 ng/mL. The assay variability was determined with 3 AFM1 standards (i.e., 0.25 ng/mL, 0.5 ng/mL, and 1.0 ng/mL), and the actual detection value was 0.2497, 0.5329, and 1.0941, respectively. For the assay variability of 3 CAP standards (i.e., 0.10 ng/mL, 0
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Hendrickson, Olga D., Elena A. Zvereva, Boris B. Dzantiev, and Anatoly V. Zherdev. "Highly Sensitive Immunochromatographic Detection of Porcine Myoglobin as Biomarker for Meat Authentication Using Prussian Blue Nanozyme." Foods 12, no. 23 (2023): 4252. http://dx.doi.org/10.3390/foods12234252.

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This study was aimed at the sensitive immunodetection of porcine myoglobin (MG) as a species-specific biomarker in meat products. The enhanced lateral flow immunoassay (LFIA) was created in the sandwich format using monoclonal antibodies (Mab) with specificity to porcine MG and labeled by Prussian blue nanoparticles (PBNPs) as peroxidase-mimicking nanozymes. Signal amplification was provided by the colored product of oxidation catalyzed by the PBNPs. Several Mab–PBNP conjugates with different antibody loads were synthesized; the one that provided the best analytical characteristics of the LFIA
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Yale, Gibson, Mani, et al. "Evaluation of an Immunochromatographic Assay as a Canine Rabies Surveillance Tool in Goa, India." Viruses 11, no. 7 (2019): 649. http://dx.doi.org/10.3390/v11070649.

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Rabies is a fatal zoonotic disease transmitted by the bite of a rabid animal. More than 95% of the human rabies cases in India are attributed to exposure to rabid dogs. This study evaluated the utility of a lateral flow immunochromatographic assay (LFA) (Anigen Rapid Rabies Ag Test Kit, Bionote, Hwaseong-si, Korea) for rapid post mortem diagnosis of rabies in dogs. Brain tissue was collected from 202 animals that were screened through the Government of Goa rabies surveillance system. The brain tissue samples were obtained from 188 dogs, nine cats, three bovines, one jackal and one monkey. In a
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Chuang, Louise, Jeng-Yang Hwang, Hen-Chang Chang, Fong-Ming Chang та Shiang-Bin Jong. "Rapid and simple quantitative measurement of α-fetoprotein by combining immunochromatographic strip test and artificial neural network image analysis system". Clinica Chimica Acta 348, № 1-2 (2004): 87–93. http://dx.doi.org/10.1016/j.cccn.2004.05.010.

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Byzova, Nadezhda A., Anatoly V. Zherdev, Elena A. Zvereva, and Boris B. Dzantiev. "Immunochromatographic Assay with Photometric Detection for Rapid Determination of the Herbicide Atrazine and Other Triazines in Foodstuffs." Journal of AOAC INTERNATIONAL 93, no. 1 (2010): 36–43. http://dx.doi.org/10.1093/jaoac/93.1.36.

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Abstract An immunochromatographic system for detection of the herbicide atrazine and its structural analogues has been developed. The assay is based on the competition of atrazine and immobilized atrazine-protein conjugate for binding with anti-atrazine monoclonal antibodies. The antibodies are immobilized on colloidal gold particles with an average diameter of 30 nm under conditions providing stability of these complexes. Concentrations of reagents and regimens of their immobilization have been optimized in order to reach the minimal limit of atrazine detection. The assay is initiated by cont
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