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Journal articles on the topic 'Immunodiagnostic panels'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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1

Esquivel-Velázquez, M., P. Ostoa-Saloma, J. Morales-Montor, R. Hernández-Bello, and C. Larralde. "Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge." Journal of Biomedicine and Biotechnology 2011 (2011): 1–11. http://dx.doi.org/10.1155/2011/516042.

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Neurocysticercosis (NCC) is a disease of the central nervous system that is considered a public health problem in endemic areas. The definitive diagnosis of this disease is made using a combination of tools that include imaging of the brain and immunodiagnostic tests, but the facilities for performing them are usually not available in endemic areas. The immunodiagnosis of NCC is a useful tool that can provide important information on whether a patient is infected or not, but it presents many drawbacks as not all infected patients can be detected. These tests rely on purified or semipurified an
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2

Vallari, Ana S., Robert K. Hickman, John R. Hackett, Catherine A. Brennan, Vincent A. Varitek, and Sushil G. Devare. "Rapid Assay for Simultaneous Detection and Differentiation of Immunoglobulin G Antibodies to Human Immunodeficiency Virus Type 1 (HIV-1) Group M, HIV-1 Group O, and HIV-2." Journal of Clinical Microbiology 36, no. 12 (1998): 3657–61. http://dx.doi.org/10.1128/jcm.36.12.3657-3661.1998.

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A rapid immunodiagnostic test that detects and discriminates human immunodeficiency virus (HIV) infections on the basis of viral type, HIV type 1 (HIV-1) group M, HIV-1 group O, or HIV-2, was developed. The rapid assay for the detection of HIV (HIV rapid assay) was designed as an instrument-free chromatographic immunoassay that detects immunoglobulin G (IgG) antibodies to HIV. To assess the performance of the HIV rapid assay, 470 HIV-positive plasma samples were tested by PCR and/or Western blotting to confirm the genotype of the infecting virus. These samples were infected with strains that r
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3

Madan, Taruna, Priyanka Priyadarsiny, Mudit Vaid, et al. "Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen of Aspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis." Clinical Diagnostic Laboratory Immunology 11, no. 3 (2004): 552–58. http://dx.doi.org/10.1128/cdli.11.3.552-558.2004.

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ABSTRACT Allergic bronchopulmonary aspergillosis (ABPA) is an immunologically complex allergic disorder caused by the fungal pathogen Aspergillus fumigatus. Elevated levels of total immunoglobulin E (IgE), specific IgE, and IgG antibodies in sera are important immunodiagnostic criteria for ABPA. International reference standards or standardized immunodiagnostic assays are not available due to a lack of well-defined diagnostic antigens. The present study was carried out to identify and evaluate the immunodiagnostic relevance of synthetic epitopic peptides of Asp f 1, a major allergen, antigen,
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4

Dai, Liping, Jitian Li, Rosalia Ortega, Wei Qian, Carlos A. Casiano, and Jian-Ying Zhang. "Preferential Autoimmune Response in Prostate Cancer to Cyclin B1 in a Panel of Tumor-Associated Antigens." Journal of Immunology Research 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/827827.

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Previous studies have demonstrated that sera from patients with prostate cancer (PCa) contain autoantibodies that react with tumor-associated antigens (TAAs). Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls. Autoantibodies to cyclin B1 were detected in 31.0% of sera from randomly selected patients with PCa versus 4.8% in sera with BPH. In the further analysis, 31.4% of sera from PCa patients at the early stage contained a
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5

Wu, Jinyu, Peng Wang, Zhuo Han, et al. "A novel immunodiagnosis panel for hepatocellular carcinoma based on bioinformatics and the autoantibody‐antigen system." Cancer Science 113, no. 2 (2021): 411–22. http://dx.doi.org/10.1111/cas.15217.

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6

Jiang, Di, Yulin Wang, Man Liu, et al. "A panel of autoantibodies against tumor-associated antigens in the early immunodiagnosis of lung cancer." Immunobiology 225, no. 1 (2020): 151848. http://dx.doi.org/10.1016/j.imbio.2019.09.007.

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7

Praekelt, Uta, Rolf Reissbrodt, Andreas Kresse, et al. "Monoclonal antibodies against all known variants of EspA: development of a simple diagnostic test for enteropathogenic Escherichia coli based on a key virulence factor." Journal of Medical Microbiology 63, no. 12 (2014): 1595–607. http://dx.doi.org/10.1099/jmm.0.076323-0.

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Enteropathogenic Escherichia coli (EPEC) are a major cause of infant diarrhoea in developing countries and a significant public health issue in industrialized countries. Currently there are no simple tests available for the diagnosis of EPEC. Serology of O-antigens is widely used routinely in many laboratories throughout the world, even though it has been known for many years to be an unreliable indicator of EPEC virulence. We have developed a simple, low-cost immunodiagnostic test based on the EspA filament, an essential virulence factor of EPEC and the related enterohaemorrhagic E. coli (EHE
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8

Wang, Peng, Chunhua Song, Weihong Xie, et al. "Evaluation of Diagnostic Value in Using a Panel of Multiple Tumor-Associated Antigens for Immunodiagnosis of Cancer." Journal of Immunology Research 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/512540.

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To determine whether a panel of multiple tumor-associated antigens (TAAs) would enhance antibody detection, the diagnostic value of autoantibodies to a panel of multiple TAAs in cancer has been evaluated. The TAAs used in this study was composed of eight TAAs including Imp1, p62, Koc, p53, C-myc, Cyclin B1, Survivin, and p16 full-length recombinant proteins. Enzyme-linked immunosorbent assay and immunoblotting were used to detect antibodies in 304 cancer sera and also 58 sera from normal individuals. The antibody frequency to any individual TAA in cancer was variable but rarely exceeded 20%. W
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9

Zhang, Hong-Fei, Jie-Jie Qin, Peng-Fei Ren, et al. "A panel of autoantibodies against multiple tumor-associated antigens in the immunodiagnosis of esophageal squamous cell cancer." Cancer Immunology, Immunotherapy 65, no. 10 (2016): 1233–42. http://dx.doi.org/10.1007/s00262-016-1886-6.

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10

Rhodes, S. G., D. Gavier-Widen, B. M. Buddle, et al. "Antigen Specificity in Experimental Bovine Tuberculosis." Infection and Immunity 68, no. 5 (2000): 2573–78. http://dx.doi.org/10.1128/iai.68.5.2573-2578.2000.

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ABSTRACT This report describes the kinetics of T-cell responses to a panel of mycobacterial antigens (PPD-M, PPD-A, ESAT-6, Ag85, 38kD, MPB64, MPB70, MPB83, hsp16.1, hsp65, and hsp70) following experimental infection of cattle with Mycobacterium bovis. Increased antigen-specific lymphocyte proliferation, gamma interferon, and interleukin-2 responses were observed in all calves following infection. Positive lymphocyte proliferation and cytokine responses to PPD-M and ESAT-6 were observed throughout the infection period studied. In contrast, responses to all other antigens were more variable and
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11

Camussone, Cecilia, Verónica Gonzalez, María S. Belluzo, et al. "Comparison of Recombinant Trypanosoma cruzi Peptide Mixtures versus Multiepitope Chimeric Proteins as Sensitizing Antigens for Immunodiagnosis." Clinical and Vaccine Immunology 16, no. 6 (2009): 899–905. http://dx.doi.org/10.1128/cvi.00005-09.

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ABSTRACT The aim of this work was to determine the best strategy to display antigens (Ags) on immunochemical devices to improve test selectivity and sensitivity. We comparatively evaluated five Trypanosoma cruzi antigenic recombinant peptides, chose the three more sensitive ones, built up chimeras bearing these selected Ags, and systematically compared by enzyme-linked immunosorbent assay the performance of the assortments of those peptides with that of the multiepitope constructions bearing all those peptides lineally fused. The better-performing Ags that were compared included peptides homol
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Margutti, Paola, Elena Ortona, Federica Delunardo, et al. "Thioredoxin peroxidase from Echinococcus granulosus: a candidate to extend the antigenic panel for the immunodiagnosis of human cystic echinococcosis." Diagnostic Microbiology and Infectious Disease 60, no. 3 (2008): 279–85. http://dx.doi.org/10.1016/j.diagmicrobio.2007.10.004.

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13

Wang, Peng, Jiejie Qin, Hua Ye, Liuxia Li, Xiao Wang, and Jianying Zhang. "Using a panel of multiple tumor‐associated antigens to enhance the autoantibody detection in the immunodiagnosis of ovarian cancer." Journal of Cellular Biochemistry 120, no. 3 (2018): 3091–100. http://dx.doi.org/10.1002/jcb.27497.

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14

LORENZO, C., J. A. LAST, and G. G. GONZÁLEZ-SAPIENZA. "The immunogenicity of Echinococcus granulosus antigen 5 is determined by its post-translational modifications." Parasitology 131, no. 5 (2005): 669–77. http://dx.doi.org/10.1017/s0031182005008309.

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Since its early introduction as a marker for the immunodiagnosis of hydatid disease, antigen 5 (Ag5) has been regarded as one of the more relevant antigens of Echinococcus granulosus, and it is still widely used in different confirmation techniques. In this work we prepared 2 recombinant forms of the antigen, namely, rAg5 (corresponding to the unprocessed polypeptide chain of the antigen) and rAg5-38s (corresponding to its 38 kDa subunit). Their antigenicities were compared to that of the native antigen using a human serum collection. There was a major drop in the reactivity of the sera, parti
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15

De Marchi, Claudia R., Javier M. Di Noia, Alberto C. C. Frasch, Vicente Amato Neto, Igor C. Almeida, and Carlos A. Buscaglia. "Evaluation of a Recombinant Trypanosoma cruzi Mucin-Like Antigen for Serodiagnosis of Chagas' Disease." Clinical and Vaccine Immunology 18, no. 11 (2011): 1850–55. http://dx.doi.org/10.1128/cvi.05289-11.

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ABSTRACTChagas' disease is caused by the protozoan parasiteTrypanosoma cruziand is one of the most important endemic problems in Latin America. Lately, it has also become a health concern in the United States and Europe. Currently, a diagnosis of Chagas' disease and the screening of blood supplies for antiparasite antibodies are achieved by conventional serological tests that show substantial variation in the reproducibility and reliability of their results. In addition, the specificity of these assays is curtailed by antigenic cross-reactivity with sera from patients affected by other endemic
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16

González-Sapienza, Gualberto, Carmen Lorenzo, and Alberto Nieto. "Improved Immunodiagnosis of Cystic Hydatid Disease by Using a Synthetic Peptide with Higher Diagnostic Value Than That of Its Parent Protein, Echinococcus granulosus Antigen B." Journal of Clinical Microbiology 38, no. 11 (2000): 3979–83. http://dx.doi.org/10.1128/jcm.38.11.3979-3983.2000.

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The assays are used for the diagnosis of hydatid disease are still imperfect. The reported diagnostic sensitivity and specificity vary greatly depending on the panel of sera used, the laboratory conducting the assay, and, more critically, the antigen used. To contribute to its standardization, we have recently ranked the diagnostic performances of the major parasite antigens and the available synthetic peptides using a large collection of serum samples. That work showed that antigen B (AgB) possesses the highest diagnostic value among these antigens. In the present work we further dissected it
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17

Rotchanapreeda, Tiwa, Pattarana Sae-Chew, Tassanee Lohnoo, et al. "Immunological Cross-Reactivity of Proteins Extracted from the Oomycete Pythium insidiosum and the Fungus Basidiobolus ranarum Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis." Journal of Fungi 7, no. 6 (2021): 474. http://dx.doi.org/10.3390/jof7060474.

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Pythiosis, a life-threatening disease caused by Pythium insidiosum, has been increasingly diagnosed worldwide. A recently developed immunochromatographic test (ICT) enables the rapid diagnosis of pythiosis. During the 3-year clinical implementation of ICT in Thailand, we collected the laboratory reports of 38 animals with suspected pythiosis and detected ICT false-positive results in three horses and a dog with basidiobolomycosis. P. insidiosum and Basidiobolus ranarum cause infections with indistinguishable clinical and microscopic features. This study investigated cross-reactive antibodies b
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18

Cuba Cuba, C. A., C. O. Torno, O. Ledesma, et al. "Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes." Revista do Instituto de Medicina Tropical de São Paulo 38, no. 6 (1996): 413–21. http://dx.doi.org/10.1590/s0036-46651996000600005.

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Diagnostic and parasite characterization and identification studies were carried out in human patients with cutaneous leishmaniasis lesions in Santiago del Estero, Northern Province of Argentina. Diagnostic procedures were biopsies of lesions for smears and inoculations in hamster, needle aspirations of material from ulcers for "in vitro" cultures. Immunodiagnostic techniques applied were IFAT-IgG and Montenegro skin test. Primary isolation of eight stocks of leishmanial parasites was achieved from patients with active lesions. All stocks were biologically characterized by their behaviour in h
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19

Sathongdejwisit, Pakornswit, Kritsada Pruksaphon, Akarin Intaramat, et al. "A Novel, Inexpensive In-House Immunochromatographic Strip Test for Cryptococcosis Based on the Cryptococcal Glucuronoxylomannan Specific Monoclonal Antibody 18B7." Diagnostics 11, no. 5 (2021): 758. http://dx.doi.org/10.3390/diagnostics11050758.

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The aim of this study was to develop a novel lateral flow immunochromatoghaphic strip test (ICT) for detecting cryptococcal polysaccharide capsular antigens using only a single specific monoclonal antibody, mAb 18B7. The mAb 18B7 is a well characterized antibody that specifically binds repeating epitopes displayed on the cryptococcal polysaccharide glucuronoxylomannan (GXM). We validated the immunoreactivities of mAb 18B7 against capsular antigens of different cryptococcal serotypes. The mAb 18B7 ICT was constructed as a sandwich ICT strip and the antibody serving in the mobile phase (colloida
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20

Duan, Yaru, Chi Cui, Cuipeng Qiu, et al. "Serum Autoantibodies against LRDD, STC1, and FOXA1 as Biomarkers in the Detection of Ovarian Cancer." Disease Markers 2022 (March 1, 2022): 1–11. http://dx.doi.org/10.1155/2022/6657820.

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Purpose. This study is aimed at evaluating serum autoantibodies against four tumor-associated antigens, including LRDD, STC1, FOXA1, and EDNRB, as biomarkers in the immunodiagnosis of ovarian cancer (OC). Methods. The autoantibodies against LRDD, STC1, FOXA1, and EDNRB were measured using an enzyme-linked immunosorbent assay (ELISA) in 94 OC patients and 94 normal healthy controls (NHC) in the research group. In addition, the diagnostic values of different autoantibodies were validated in another independent validation group, which comprised 136 OC patients, 136 NHC, and 181 patients with beni
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21

Mu, Yi, Catherine A. Gordon, Remigio M. Olveda, et al. "Identification of a linear B-cell epitope on the Schistosoma japonicum saposin protein, SjSAP4: Potential as a component of a multi-epitope diagnostic assay." PLOS Neglected Tropical Diseases 16, no. 7 (2022): e0010619. http://dx.doi.org/10.1371/journal.pntd.0010619.

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Background Schistosoma japonicum is one of three major species of blood flukes causing schistosomiasis, a disease, which continues to be a major public health issue in the Philippines. SjSAP4, a member of a multigene family of saposin-like proteins, is a recognized immunodiagnostic biomarker for schistosomiasis japonica. This study aimed to identify linear B-cell epitopes on SjSAP4 and to validate their potential as components of a multi-epitope assay for the serological diagnosis of schistosomiasis japonica. Methodology SjSAP4-dereived peptides were expressed as GST-peptide-fused proteins and
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Lawrenz, M. B., J. M. Hardham, R. T. Owens, et al. "Human Antibody Responses to VlsE Antigenic Variation Protein of Borrelia burgdorferi." Journal of Clinical Microbiology 37, no. 12 (1999): 3997–4004. http://dx.doi.org/10.1128/jcm.37.12.3997-4004.1999.

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VlsE is a 35-kDa surface-exposed lipoprotein of Borrelia burgdorferi that was shown previously to undergo antigenic variation through segmental recombination of silent vlscassettes with vlsE during experimental mouse infections. Previous data had indicated that sera from North American Lyme disease patients and experimentally infected animals contained antibodies reactive with VlsE. In this study, sera from patients with Lyme disease, syphilis, and autoimmune conditions as well as from healthy controls were examined for reactivity with VlsE by Western blotting and enzyme-linked immunosorbent a
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Mutter, Jurik A., Mohammad Shahrokh Esfahani, Joseph Schroers-Martin, et al. "Inferred Gene Expression By Cell-Free DNA Profiling Allows Noninvasive Lymphoma Classification." Blood 142, Supplement 1 (2023): 245. http://dx.doi.org/10.1182/blood-2023-186853.

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Background : Current diagnostic criteria for mature B-cell tumors require a combination of histopathological, immunophenotypic, cytogenetic, and genetic evaluations on tissue biopsy specimens (Campo, Blood 2022; Alaggio, Leuk 2022). Despite this battery of techniques, substantial heterogeneity remains in specific subgroups that can be further resolved using additional molecular diagnostic methods, including gene expression profiling (GEP). For example, among aggressive B-cell tumors, a subset recognized as high-grade B-cell lymphomas can be identified as harboring MYC+BCL2 lesions by FISH (HGB
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SPIROSKI, Mirko Zh. "IMMUNOLOGYCAL APPROACH TO THE DIAGNOSIS OF AUTISM." August 22, 2015. https://doi.org/10.5281/zenodo.28989.

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Tang, Cong, Patrícia Corredeira, Sandra Casimiro, et al. "Immunodiagnostic plasma amino acid residue biomarkers detect cancer early and predict treatment response." Nature Communications 16, no. 1 (2025). https://doi.org/10.1038/s41467-025-61685-2.

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Abstract The immune response to tumour development is frequently targeted with therapeutics but remains largely unexplored in diagnostics, despite being stronger for early-stage tumours. We present an immunodiagnostic platform to detect this. We identify a panel of amino acid residue biomarkers providing a signature of cancer-specific immune activation associated with tumour development and distinct from autoimmune and infectious diseases, measurable optically in neat blood plasma, and validate within N = 170 participants. By measuring the total concentrations of cysteine, free cysteine, lysin
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Ribeiro, Anna Julia, Kamila Alves Silva, Lucas da Silva Lopes, et al. "The use of peptides for immunodiagnosis of human Chagas disease." Amino Acids 56, no. 1 (2024). http://dx.doi.org/10.1007/s00726-024-03394-6.

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AbstractChagas disease, caused by the protozoa Trypanosoma cruzi, continues to be a serious public health problem in Latin America, worsened by the limitations in its detection. Given the importance of developing new diagnostic methods for this disease, the present review aimed to verify the number of publications dedicated to research on peptides that demonstrate their usefulness in serodiagnosis. To this end, a bibliographic survey was conducted on the PubMed platform using the keyword “peptide” or “epitope” combined with “Chagas disease” or “Trypanosoma cruzi”; “diagno*” or “serodiagnosis”
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Silva, Bruno B., Eduarda N. F. N. Santos, Lucelina S. Araújo, et al. "Plant Expression of Hydrophobin Fused K39 Antigen for Visceral Leishmaniasis Immunodiagnosis." Frontiers in Plant Science 12 (May 31, 2021). http://dx.doi.org/10.3389/fpls.2021.674015.

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Visceral leishmaniasis is a Neglected Tropical Disease of high mortality caused by the protozoan Leishmania infantum. Its transmission cycle is complex, and it has in the domestic dog its main reservoir. The diagnostic tests currently used rely on prokaryotic systems’ proteins, but their low sensitivity increases the disease’s burden. The plant transient expression of recombinant proteins allows the production of complex antigens. However, this system has limited competitiveness against the bacterial production of purified antigens. Thus, we have shown that the L. infantum K39 antigen’s fusion
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Wang, Bo, Christien A. Kluwe, Oana I. Lungu, et al. "Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining." Scientific Reports 5, no. 1 (2015). http://dx.doi.org/10.1038/srep13926.

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Abstract The ongoing evolution of Ebolaviruses poses significant challenges to the development of immunodiagnostics for detecting emergent viral variants. There is a critical need for the discovery of monoclonal antibodies with distinct affinities and specificities for different Ebolaviruses. We developed an efficient technology for the rapid discovery of a plethora of antigen-specific monoclonal antibodies from immunized animals by mining the VH:VL paired antibody repertoire encoded by highly expanded B cells in the draining popliteal lymph node (PLN). This approach requires neither screening
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Wang, Shuaibing, Jiejie Qin, Hua Ye, et al. "Using a panel of multiple tumor-associated antigens to enhance autoantibody detection for immunodiagnosis of gastric cancer." OncoImmunology, March 27, 2018, e1452582. http://dx.doi.org/10.1080/2162402x.2018.1452582.

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Verma, Priyanka, Santwana Bhatnagar, Pradeep Kumar, et al. "Analysis of antibody response (IgM, IgG, IgG3) to Chikungunya virus using panel of peptides derived from envelope protein for serodiagnosis." Clinical Chemistry and Laboratory Medicine (CCLM) 52, no. 2 (2014). http://dx.doi.org/10.1515/cclm-2013-0363.

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AbstractMany epidemic outbreaks of Chikungunya fever (CHIKF) have been reported throughout the world including India after its reemergence in 2005. The immuno protective role of envelope proteins during Chikungunya virus (CHIKV) infection has been reported. With the aim of identifying the immunodominant epitopes within the envelope protein we investigated the detailed analysis of fine specificity of antibody response in different individuals during CHIKV infection.The peptides corresponding to the full length of E1, E2 and E3 proteins of S27 strain of CHIKV were synthesized and their seroreact
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Luo, Manli, Songmei Wu, Yan Ma, et al. "Evaluating a Panel of Autoantibodies Against Tumor-Associated Antigens in Human Osteosarcoma." Frontiers in Genetics 13 (April 25, 2022). http://dx.doi.org/10.3389/fgene.2022.872253.

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Background: The aim of this study was to identify a panel of candidate autoantibodies against tumor-associated antigens in the detection of osteosarcoma (OS) so as to provide a theoretical basis for constructing a non-invasive serological diagnosis method in early immunodiagnosis of OS.Methods: The serological proteome analysis (SERPA) approach was used to select candidate anti-TAA autoantibodies. Then, indirect enzyme-linked immunosorbent assay (ELISA) was used to verify the expression levels of eight candidate autoantibodies in the serum of 51 OS cases, 28 osteochondroma (OC), and 51 normal
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Song, Yujing, Erin Sandford, Yuzi Tian, et al. "Rapid single-molecule digital detection of protein biomarkers for continuous monitoring of systemic immune disorders." Blood, December 4, 2020. http://dx.doi.org/10.1182/blood.2019004399.

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Digital protein assays have great potential to advance immunodiagnostics because of their single-molecule sensitivity, high precision, and robust measurements. However, translating digital protein assays to acute clinical care has been challenging because it requires their deployment with a rapid turnaround. Herein, we present a technology platform for ultra-fast digital protein biomarker detection by employing single-molecule counting of immune-complex formation events at an early, pre-equilibrium state. This method, which we term "pre-equilibrium digital enzyme-linked immunosorbent assay" (P
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Lester, Sandra N., Megan Stumpf, Brandi D. Freeman, et al. "Examination of SARS-CoV-2 serological test results from multiple commercial and laboratory platforms with an in-house serum panel." Access Microbiology 6, no. 2 (2024). http://dx.doi.org/10.1099/acmi.0.000463.v4.

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Severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) is a novel human coronavirus that was identified in 2019. SARS-CoV-2 infection results in an acute, severe respiratory disease called coronavirus disease 2019 (COVID-19). The emergence and rapid spread of SARS-CoV-2 has led to a global public health crisis, which continues to affect populations across the globe. Real time reverse transcription polymerase chain reaction (rRT-PCR) is the reference standard test for COVID-19 diagnosis. Serological tests are valuable tools for serosurveillance programs and establishing correlates
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Kasturi, Kuppuswamy N., and Tomas Drgon. "Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples." Applied and Environmental Microbiology 83, no. 14 (2017). http://dx.doi.org/10.1128/aem.00644-17.

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ABSTRACT The methods currently used for detecting Salmonella in environmental samples require 2 days to produce results and have limited sensitivity. Here, we describe the development and validation of a real-time PCR Salmonella screening method that produces results in 18 to 24 h. Primers and probes specific to the gene invA, group D, and Salmonella enterica serovar Enteritidis organisms were designed and evaluated for inclusivity and exclusivity using a panel of 329 Salmonella isolates representing 126 serovars and 22 non-Salmonella organisms. The invA- and group D-specific sets identified a
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Moreira, Gustavo Marçal Schmidt Garcia, Sabine Gronow, Stefan Dübel, et al. "Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection of Listeria monocytogenes." Frontiers in Public Health 10 (March 15, 2022). http://dx.doi.org/10.3389/fpubh.2022.712657.

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Listeria monocytogenes is the causative agent of listeriosis, a highly lethal disease initiated after the ingestion of Listeria-contaminated food. This species comprises different serovars, from which 4b, 1/2a, and 1/2b cause most of the infections. Among the different proteins involved in pathogenesis, the internalins A (InlA) and B (InlB) are the best characterized, since they play a major role in the enterocyte entry of Listeria cells during early infection. Due to their covalent attachment to the cell wall and location on the bacterial surface, along with their exclusive presence in the pa
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Arastoo, Mohammad, Lewis K. Penny, Richard Lofthouse, et al. "High-affinity antibodies specific to the core region of the tau protein exhibit diagnostic and therapeutic potential for Alzheimer’s disease." Alzheimer's Research & Therapy 16, no. 1 (2024). http://dx.doi.org/10.1186/s13195-024-01561-1.

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Abstract Background Recent advances in blood-based biomarker discovery are paving the way for simpler, more accessible diagnostic tools that can detect early signs of Alzheimer’s disease (AD). Recent successes in the development of amyloid-targeting immunotherapy approaches mark an important advancement in providing new options for the treatment of AD. We have developed a set of high-affinity monoclonal antibodies (mAbs) to tau protein that have the potential as tools for diagnosis and treatment of AD. Methods Sheep were immunised with either full-length tau (1-441) or truncated paired helical
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Riabkova, Natalia S., Agnessa P. Bogomolova, Alexander E. Kogan, et al. "Interaction of heparin with human cardiac troponin complex and its influence on the immunodetection of troponins in human blood samples." Clinical Chemistry and Laboratory Medicine (CCLM), May 14, 2024. http://dx.doi.org/10.1515/cclm-2024-0066.

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Abstract Objectives Heparin is a highly charged polysaccharide used as an anticoagulant to prevent blood coagulation in patients with presumed myocardial infarction and to prepare heparin plasma samples for laboratory tests. There are conflicting data regarding the effects of heparin on the measurement of cardiac isoforms of troponin I (cTnI) and troponin T (cTnT), which are used for the immunodiagnosis of acute myocardial infarction. In this study, we investigated the influence of heparin on the immunodetection of human cardiac troponins. Methods Gel filtration (GF) techniques and sandwich fl
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38

Tiwari, Ragini I., Shraddha S. Bhullar, Nitin H. Chandak, et al. "Novel Synthetic Peptide-based Enzyme Linked Immunosorbent Assay for Antibody Detection in Viral Infections of the Central Nervous System." JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2022. http://dx.doi.org/10.7860/jcdr/2022/52812.16573.

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Introduction: Viral infections of Central Nervous System (CNS) are associated with severe neurological sequelae and can lead to significant morbidity if not adequately diagnosed and treated. An immunological assay detecting antibodies in the Cerebrospinal Fluid (CSF) of the patients is widely used for diagnosis of viral infections of the CNS. In the present study, diagnostic efficacy of in-house designed synthetic peptide based Enzyme Linked Immunosorbent Assay (ELISA) was evaluated for detection of antibodies against neurotropic viruses panel such as Cytomegalovirus (CMV), Epstein-Barr virus
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39

"P98 Blood-based biomarkers of extracellular matrix turnover are elevated in patients with psoriasis compared with healthy controls." British Journal of Dermatology 191, Supplement_3 (2024). https://doi.org/10.1093/bjd/ljae360.128.

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Abstract The extracellular matrix (ECM) is a non-cellular network in all tissues and organs. Collagens are the most abundant proteins in the ECM, and their role is to maintain the tissue architecture of the different layers of the skin. Blood-based biomarkers of ECM fragments have previously been shown to be diagnostic and pharmacodynamic in psoriatic arthritis (PsA) and spondylarthritis. This study aimed to investigate whether ECM biomarkers can differentiate between patients with moderate-to-severe psoriasis (PSO) and healthy controls (HCs) and to determine the potential of ECM biomarkers as
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