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Journal articles on the topic 'Immunohistochemical method'

Author: Grafiati
Published: 28 May 2022
Last updated: 31 July 2025

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1

Stancu, Adrian, Alina Ghise, Marius Pente, et al. "Immunohistochemical Diagnosis Method in Parvovirus." Revista de Chimie 71, no. 7 (2020): 491–96. http://dx.doi.org/10.37358/rc.20.7.8268.

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10 cases of histopathologically suggestive lesions for parvovirus in the canine species were examined by the avidin-biotin-peroxidase complex. For this, a mouse monoclonal antibody was used. Following the immunohistochemical examination, positive results were obtained in 80% (8 dogs) of processed samples. The most frequent positive immunohistochemical reaction was observed in macrophages, lymphocytes, necrotic cells of the intestinal crypt, evidence of parvoviral antigen is reliable for determining the etiology of the disease if parvovirus is suspected on the basis of the lesions. In order to
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2

Alanen, S., M. J. Nissinen, T. Karhunen, and P. Panula. "An immunohistochemical method for tele-methylhistamine." Agents and Actions 36, S2 (1992): C390—C393. http://dx.doi.org/10.1007/bf01997382.

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3

Pospiech, M., B. Tremlová, E. Renčová, and Z. Randulová. "Immunohistochemical detection of soya protein – optimisation and verification of the method." Czech Journal of Food Sciences 27, No. 1 (2009): 11–19. http://dx.doi.org/10.17221/2848-cjfs.

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A functional immunohistochemical method for soya proteins detection was developed. The procedure is based on the avidin-biotin complex (ABC) method that attains sufficient sensitivity. The method was verified by the analysis of the model samples of different forms of soya additives containing various concentrations of soya isolate. The detection limit of soya present in the model samples was 0.5%. Different possibilities of the background staining were tested. The best results were obtained with the background staining according to Calleja. The results were confirmed by the accredited indirect
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Loreto, Carla, and Rosario Caltabiano. "Immunohistochemical Expression." Applied Sciences 11, no. 1 (2021): 360. http://dx.doi.org/10.3390/app11010360.

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Immunohistochemistry (IHC) is an ancillary method, widely used in pathologist practice, that allows to identify diagnostic and prognostic/predictive therapeutic response protein markers on tissue samples by the use of specific monoclonal antibodies and chromogenic substances that guarantee the visualization of the antibody–antigene binding complex under the light microscope [...]
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Pospiech, M., B. Tremlová, E. Renčová, RandulováZ, Z. Řezáčová Lukášková, and J. Pokorná. "Comparison of the results of the ELISA, histochemical, and immunohistochemical detection of soya proteins in meat products    ." Czech Journal of Food Sciences 29, No. 5 (2011): 471–79. http://dx.doi.org/10.17221/209/2010-cjfs.

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This work compares the commonly used immunochemical methods for soya protein detection and alternative microscopic methods. Immunochemical methods were represented by the competitive ELISA method. Histochemical and immunohistochemical methods were used for microscopical examination. From a group of 252 meat products, each sample was examined for soya proteins by ELISA, histochemical, and immunohistochemical methods. The products came from the following categories: cooked sausages, ham, dry cooked sausages, and fermented sausages. The results showed that the highest accuracy was achieved by imm
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Řezáčová-Lukášková, Z., B. Tremlová, M. Pospiech, E. Renčová, and Z. Randulová. "Immunohistochemical detection of wheat protein in model samples." Czech Journal of Food Sciences 28, No. 6 (2010): 516–19. http://dx.doi.org/10.17221/1838-cjfs.

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The study focused on the optimisation of immunohistochemical examination for gluten content detection in model samples (pork meat with wheat semi-smooth flour, pork meat with wheat protein edible vital). The best results were achieved with immunohistochemical method based on ABC (avidin–biotin complex) method utilising polyclonal antibodies diluted 1:1000. The results demonstrate that for pure wheat protein detection, the utilisation of immunohistochemical detection, which can detect as little as 0.1% of the added wheat protein, is more advantageous, while the commonly used ELISA met
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Novakovic, Zorica, Sofija Katic-Radivojevic, and Vera Todorovic. "Implementation of immunohistochemical methods in diagnostics of bovine sarcocystosis." Veterinarski glasnik 60, no. 3-4 (2006): 175–86. http://dx.doi.org/10.2298/vetgl0604175n.

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Bovine sarcocystosis was examined in three age categories of cattle using direct and immunohistochemical methods. The direct methods of compression, trypsin digestion and a histological method were applied, and the applied immunohistochemical methods included peroxidase antiperoxidase (PAP), the highly sensitive streptavidin biotin immunoperoxidase complex technique (LSAB+) and indirect immunofluorescence (IF). Primary rabbit antiserum obtained for the zoites Sarcocystis cruzi syn. S. bovicanis was used for all immunochemical and immunohistochemical examinations. Cross reactivity was determine
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8

Ellis, John A., Karen Martin, Norman G. Robert, and Deborah M. Haines. "Comparison of Detection Methods for Bovine Viral Diarrhea Virus in Bovine Abortions and Neonatal Death." Journal of Veterinary Diagnostic Investigation 7, no. 4 (1995): 433–36. http://dx.doi.org/10.1177/104063879500700402.

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The objective of this study was to examine a monoclonal antibody-based immunohistochemical staining method for its efficacy in diagnosis of bovine virus diarrhea virus (BVDV)-induced abortion and neonatal calf death. This method was compared to viral isolation and immunofluorescence staining of frozen tissue sections. Tissues from 105 cases, 53 fetuses and 52 neonates, were tested by the 3 methods. There were significant numbers of both false negatives and false positives with the immunofluorescence method and significant numbers of false negatives with the viral isolation method. Of the metho
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ANDREI, Lucian Sorin, Adriana Corina ANDREI, Vlad HERLEA, Florentina ALMARI, and Aleandru MICU. "Retroperitoneal Precaval Pecoma – an Incidental Finding." Medicina Moderna - Modern Medicine 31, no. 4 (2024): 359–62. https://doi.org/10.31689/rmm.2024.31.4.359.

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PEComas are rare mesenchymal tumors of histologically and immunohistochemically distinctive perivascular epithelioid cells. Most of these tumours are benign, but in malignancies the prognosis is usually very poor. The clinical presentation of this tumours is nonspecific and the imagistic eamination is usually not sufficiently sensitive to establish a diagnosis. The best diagnostic method is histopathological eamination combined with immunohistochemical eamination. Treatment of this tumors is surgery followed by chemotherapy (in case of malignancy). We present the case of a 43-year-old female p
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10

Wong, K. T., J. Vadivelu, S. D. Puthucheary, and K. L. Tan. "An immunohistochemical method for the diagnosis of melioidosis." Pathology 28, no. 2 (1996): 188–91. http://dx.doi.org/10.1080/00313029600169853.

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11

Yamagishi, Masuo, Satoshi Hasegawa, Sugata Takahashi, Yuichi Nakano, and Toshihiko Iwanaga. "Immunohistochemical Method for the Diagnosis of Olfactory Disturbance." Acta Oto-Laryngologica 103, no. 1-2 (1987): 145–50. http://dx.doi.org/10.3109/00016488709134710.

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12

Tanaka, Shinya. "A newly developed rapid immunohistochemical method: R-IHC." Pathology 46 (2014): S25—S26. http://dx.doi.org/10.1097/01.pat.0000454137.04707.27.

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13

Alatas, Fatima Safira, Kouji Masumoto, Kouji Nagata, et al. "Diagnostic challenges of hypoganglionosis based on immunohistochemical method." Translational Pediatrics 12, no. 6 (2023): 1161–69. http://dx.doi.org/10.21037/tp-22-592.

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14

Petrášová, Michaela, Matěj Pospiech, Zuzana Řezáčová Lukášková, and Bohuslava Tremlová. "Comparison of new immunofluorescence method for detection of soy protein in meat products with immunohistochemical, histochemical, and ELISA methods." Acta Veterinaria Brno 83, no. 10 (2014): S65—S69. http://dx.doi.org/10.2754/avb201483s10s65.

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Soy proteins are commonly used in the food industry thanks to their technological properties. However, soy is, along with cow’s milk, eggs, wheat, peanuts, tree nuts, fish, crustaceans, and molluscs, responsible for around 90% of food allergies, and is also one of the foodstuffs that can cause anaphylaxis. The aim of this work was to compare the immunofluorescence method for the detection of soy protein in meat products purchased from the retail market with other microscopic methods (immunohistochemical and histochemical), with the ELISA reference method and with the confirmatory results. With
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15

Russo, D., A. Ambrosino, A. Vittoria, and A. Cecio. "Signal amplification by combining two advanced immunohistochemical techniques." European Journal of Histochemistry 47, no. 4 (2009): 379. http://dx.doi.org/10.4081/850.

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The immunohistochemical techniques known as EnVision™+ System (EVS) and Mirror Image Complementary Antibody (MICA) were recently introduced into laboratory practice because of their high sensitivity. In this paper these techniques were compared and their sequences combined to obtain a new method possibly more sensitive than the original ones. The immunohistochemical staining employing the avidin-biotin complex (ABC), largely used as routine, was adopted as a term of comparison. Samples from the small and large intestine of pigs and sheep were fixed in Bouin and embedded in Paraplast. The prima
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Thoresen, Ove F., Knut Falk, and Øystein Evensen. "Comparison of Immunohistochemistry, Acid-Fast Staining, and Cultivation for Detection of Mycobacterium Paratuberculosis in Goats." Journal of Veterinary Diagnostic Investigation 6, no. 2 (1994): 195–99. http://dx.doi.org/10.1177/104063879400600210.

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Forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. Culture of the specimens for Mycobacterium paratuberculosis was compared with Ziehl-Neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a M. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. Immunohistochemical techniques detected most positive samples and produced more clearly visible reactions than did acid-fast staining. Eighteen of 47 samples were positive by immunohistochemic
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17

Moran, M. M., R. J. Siegel, J. W. Said, and M. C. Fishbein. "Demonstration of myoglobin and CK-M in myocardium. Comparison of five fixation methods and three immunohistochemical techniques." Journal of Histochemistry & Cytochemistry 33, no. 11 (1985): 1110–15. http://dx.doi.org/10.1177/33.11.3902962.

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The results of immunohistochemical staining vary depending on the tissue, fixative, antigen-antibody system, and immunohistochemical staining methods used. The purpose of this study was to evaluate the effect of different methods of fixation, different antigen-antibody systems, and different immunohistochemical methods on immunohistochemical staining of myocardium. Samples of normal fresh canine myocardium from six dogs were fresh frozen and fixed in 10% neutral buffered formalin, Bouin's, Bayley's and Carnoy's fixatives. Immunohistochemical staining for myoglobin and creatine kinase M was per
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18

Stafford, V. V., E. B. Streltsova, A. D. Zaberezhny, T. I. Aliper, and A. M. Gulyukin. "Immunohistochemical Method for Detection PCV-2 Antigen in Pigs." IOP Conference Series: Earth and Environmental Science 666, no. 5 (2021): 052017. http://dx.doi.org/10.1088/1755-1315/666/5/052017.

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19

Raheem, O., T. Suominen, S. Penttilä, H. Haapasalo, and B. Udd. "P2.36 Immunohistochemical diagnostic method for chloride channel mutated myotonia." Neuromuscular Disorders 20, no. 9-10 (2010): 629. http://dx.doi.org/10.1016/j.nmd.2010.07.108.

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20

Řezáčová Lukášková, Zuzana, Matěj Pospiech, Bohuslava Tremlová, Eva Renčová, and Michaela Petrášová. "Quantitative immunohistochemical method for detection of wheat protein in model sausage." Acta Veterinaria Brno 83, no. 10 (2014): S71—S76. http://dx.doi.org/10.2754/avb201483s10s71.

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Since gluten can induce coeliac symptoms in hypersensitive consumers with coeliac disease, it is necessary to label foodstuffs containing it. In order to label foodstuffs, it is essential to find reliable methods to accurately determine the amount of wheat protein in food. The objective of this study was to compare the quantitative detection of wheat protein in model sausages by ELISA and immunohistochemical methods. Immunohistochemistry was combined with stereology to achieve quantitative results. High correlation between addition of wheat protein and compared methods was confirmed. For ELISA
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21

Liu, Chia-Hsing, Chih-Hung Lin, Min-Jan Tsai, Yu-Hsuan Chen, Sheau-Fang Yang, and Kun-Bow Tsai. "Melanin Bleaching With Warm Hydrogen Peroxide and Integrated Immunohistochemical Analysis: An Automated Platform." International Journal of Surgical Pathology 26, no. 5 (2018): 410–16. http://dx.doi.org/10.1177/1066896918756998.

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Objective. Diagnosing melanocytic lesions is among the most challenging problems in the practice of pathology. The difficulty of physically masking melanin pigment and the similarity of its color to commonly used chromogens often complicate examination of the cytomorphology and immunohistochemical staining results for tumor cells. Melanin bleach can be very helpful for histopathological diagnosis of heavily pigmented melanocytic lesions. Although various depigmentation methods have been reported, no standardized methods have been developed. This study developed a fully automated platform that
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22

Do Van, A., Chu Nguyen Van, Lang Ngo Van, Thu Le Van, and Quan Duong Hong. "Assessment of results in manual immunohistochemical and automated immunohistochemical staining of estrogen receptor in breast cancer." Journal of Health and Development Studies 07, no. 05 (2023): 71–78. http://dx.doi.org/10.38148/jhds.0705skpt22-089.

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Objectives: Determine the rate and extent of ER expression in BC by manual IHC staining method compared with automatic machine staining; Comment on some factors affecting the quality of manual IHC staining with ER compared to automatic machine staining. Subjects: 33 cases of BC and results of IHC test with ER+ marker at K1 hospital in 2022. Research method: Experimental research. Results: With IHC staining by automatic machine, the positive rate is 100%, of which positive 2(+) accounts for the highest rate with 51.5%, then positive 3(+) and positive 1(+) is 39.4 and 9.1%, respectively. With ma
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Fan, Lin, Yanyan Tian, Rong Yin, et al. "Enzyme catalysis enhanced dark-field imaging as a novel immunohistochemical method." Nanoscale 8, no. 16 (2016): 8553–58. http://dx.doi.org/10.1039/c5nr08232c.

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24

Malisius, Rainer, Hartmut Merz, Boris Heinz, Evariste Gafumbegete, Britta U. Koch, and Alfred C. Feller. "Constant Detection of CD2, CD3, CD4, and CD5 in Fixed and Paraffin-embedded Tissue Using the Peroxidase-mediated Deposition of Biotin-Tyramide." Journal of Histochemistry & Cytochemistry 45, no. 12 (1997): 1665–72. http://dx.doi.org/10.1177/002215549704501210.

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Immunohistochemical methods are widely used for diagnostic purposes in histopathology. However, the use of most monoclonal anti-leukocyte antibodies is limited to frozen tissues. Initially, it was believed that formalin fixation in particular, which is the gold standard for morphological tissue preservation, destroys most of the antigen binding sites. In recent years, protease digestion and the introduction of microwave techniques have significantly enhanced the sensitivity of immunohistochemical techniques, and a variety of hidden antigen sites in formalin-fixed tissue have been retrieved for
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Karataş, Özhan, Gökhan Akçakavak, Murat Eren, et al. "Determination of 8-OHdG and 4-HNE Expressions in Sheep with Hepatic Lipidosis by Immunohistochemical Method." Manas Journal of Agriculture Veterinary and Life Sciences 15, no. 1 (2025): 9–15. https://doi.org/10.53518/mjavl.1572721.

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Lipidosis is generally defined as the accumulation of triglycerides in limited droplets within the cytoplasm of parenchymal cells. Lipidosis occurs due to toxic, chemical, infectious, and metabolic causes. This study aimed to reveal the local 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 4-hydroxynonenal (4-HNE) expressions immunohistochemically according to the severity of the disease in sheep with hepatic lipidosis. The study material consisted of a total of 30 male sheep livers, including 6 healthy and 24 with hepatic lipidosis. After the liver samples were fixed in 10% formaldehyde solution, th
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BEREZSKY, OLEH, OLEH PITSUN, TAMARA DATSKO, BOHDAN DERYSH, and GRYGORY MELNYK. "BREAST CANCER IMMUNOHISTOLOGICAL IMAGING DATABASE." Computer systems and information technologies, no. 1 (April 14, 2022): 75–82. http://dx.doi.org/10.31891/csit-2022-1-10.

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Breast cancer is the most common pathology among women. The death rate from breast cancer among women remainshigh. Early diagnosis and individual therapy are effective ways to extend people's lives. The main diagnostic methods arecytological, histological, and immunohistochemical. The cytological method allows assessing the qualitative and quantitativechanges in cells, as well as identifying extra- and intracellular inclusions and microorganisms. The histological method allows you toexplore changes in the location of groups of cells in a particular tissue. The immunohistochemical method is bas
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Thür, B., M. Hilbe, M. Strasser, and F. Ehrensperger. "Immunohistochemical diagnosis of pestivirus infection associated with bovine and ovine abortion and perinatal death." American Journal of Veterinary Research 58, no. 12 (1997): 1371–75. http://dx.doi.org/10.2460/ajvr.1997.58.12.1371.

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SUMMARY Objective To establish a reliable, rapid, economical method for detection of pestivirus infection in bovine and ovine fetuses and to examine participation of these viruses in abortions and neonatal mortality. Animals 213 bovine and 31 ovine fetuses, as well as 36 newborn calves and 25 lambs, which had died within 3 days after birth, were tested for bovine viral diarrhea virus (BVDV) and border disease virus by use of different methods. Procedure Detection of BVDV in fetuses was performed by immunohistochemical methods, using a panel of monoclonal antibodies against pestivirus antigens
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Silveira, Tatiany, Emerson Veloso, Ivy Gonçalves, and Enio Ferreira. "Use of Giemsa staining for the immunohistochemical counterstaining in canine melanomas: an “old and forgotten” method." Brazilian Journal of Veterinary Pathology 13, no. 1 (2020): 17–20. http://dx.doi.org/10.24070/bjvp.1983-0246.v13i1p17-20.

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Abbuhl, M. F., and M. E. Velasco. "An economical minichamber for immunohistochemical incubation." Journal of Histochemistry & Cytochemistry 33, no. 2 (1985): 162–64. http://dx.doi.org/10.1177/33.2.2578500.

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A simple and economical "slide-minichamber" method for incubating tissue sections with antisera in immunohistochemical (peroxidase-antiperoxidase) staining procedures is described. The technique requires only materials routinely used in the laboratory. The method permits prolonged incubation of tissue sections with antiserum at 4 degrees C or at room temperature, use of small quantities of antiserum, and simultaneous incubation of two tissue sections with the same small quantity of antiserum, thereby allowing use of very dilute antisera and conservation of antisera when availability is limited
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Linsenmayer, T. F., J. M. Fitch, and T. M. Schmid. "Multiple-reaction cycling: a method for enhancement of the immunochemical signal of monoclonal antibodies." Journal of Histochemistry & Cytochemistry 36, no. 8 (1988): 1075–78. http://dx.doi.org/10.1177/36.8.3292645.

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Most current studies using immunochemical and immunohistochemical procedures to detect antigen-antibody complexes employ some type of indirect method. Such procedures afford signal amplification because several marker-conjugate molecules can bind to each primary antibody molecule. We have observed that for monoclonal antibodies an even greater amplification can be afforded simply by performing two (or more) reaction cycles (i.e., primary antibody, secondary antibody-primary antibody, secondary antibody-etc). In the present report, we demonstrate the utility of this method for immunohistochemic
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Sandoval, C., and B. Vásquez. "Evaluation of Immunohistochemical Reactivity with Avidin-Biotin Complex (ABC) Method." International Journal of Medical and Surgical Sciences 3, no. 3 (2018): 909–18. http://dx.doi.org/10.32457/ijmss.2016.024.

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Immunohistochemistry is any technique that can detect cellular and extracellular components in situ by means of specific antibodies, using enzymatic detection systems. Among immunohistochemical methods, the technique of avidin - biotin complex (ABC) is widely used because of its high sensitivity. The aim of this study was to evaluate the immunohistochemical reactivity of the 4C4.9 antibody for detection of S-100 protein using the ABC method. For the evaluation of immunohistochemical reactivity 2 biopsies of human skin were used with histopathological diagnosis of ulcerated malignant melanoma a
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Stancu, Adrian. "Use of the Immunohistochemical Method in Feline Infectious Peritonitis Diagnosis." Revista de Chimie 70, no. 1 (2019): 147–48. http://dx.doi.org/10.37358/rc.19.1.6870.

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PIF was first described by Holzworth in 1963, but is currently reported to be one of the most feared feline diseases in many countries, due to the mortality it can produce, especially among cats of improved breeds and from breeders.
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Nakajima, Kazuyuki. "A simple immunohistochemical method for detecting microglia in rat brain." Gazette of Medical Sciences 1, no. 6 (2020): 22–29. http://dx.doi.org/10.46766/thegms.neuro.20120401.

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Surpat (Hulea), Anca Sofiana, Viorel Herman, Iosif Marincu, Narcisa Mederle, and Ovidiu Alexandru Mederle. "Immunohistochemical method for identification of Lawsonia intracellularis infection in pigs." Journal of Biotechnology 208 (August 2015): S100. http://dx.doi.org/10.1016/j.jbiotec.2015.06.314.

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Xiao, Jingnan, Zongping Xia, Anuradha Pradhan, Qiong Zhou, and Yuechueng Liu. "An immunohistochemical method that distinguishes free from complexed SNAP-25." Journal of Neuroscience Research 75, no. 1 (2003): 143–51. http://dx.doi.org/10.1002/jnr.10840.

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Durán-Díaz, Iván, Auxiliadora Sarmiento, Irene Fondón, Clément Bodineau, Mercedes Tomé, and Raúl V. Durán. "A Robust Method for the Unsupervised Scoring of Immunohistochemical Staining." Entropy 26, no. 2 (2024): 165. http://dx.doi.org/10.3390/e26020165.

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Immunohistochemistry is a powerful technique that is widely used in biomedical research and clinics; it allows one to determine the expression levels of some proteins of interest in tissue samples using color intensity due to the expression of biomarkers with specific antibodies. As such, immunohistochemical images are complex and their features are difficult to quantify. Recently, we proposed a novel method, including a first separation stage based on non-negative matrix factorization (NMF), that achieved good results. However, this method was highly dependent on the parameters that control s
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Cao, Ting, Shijie Yi, Xuefeng Yang, and Qing Wu. "Clinical Significance of Polo-Like Kinase 4 as a Marker for Advanced Tumor Stage and Dismal Prognosis in Patients With Surgical Gastric Cancer." Technology in Cancer Research & Treatment 19 (January 1, 2020): 153303382093553. http://dx.doi.org/10.1177/1533033820935531.

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Objective: This study aimed to investigate the correlation of polo-like kinase 4 with clinicopathological features and survival profiles in patients with gastric cancer. Methods: This retrospective study was conducted based on the clinical data from 289 eligible patients with gastric cancer who received resection. Polo-like kinase 4 expression in adjacent tissue and tumor tissue was determined by immunohistochemical assay and semiquantified scoring method using immunohistochemical score by staining intensity score multiplying staining density score. Based on the total immunohistochemical score
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Galuske, R. A., J. A. Delius, and W. Singer. "Lucifer yellow filling of immunohistochemically pre-labeled neurons: a new method to characterize neuronal subpopulations." Journal of Histochemistry & Cytochemistry 41, no. 7 (1993): 1043–52. http://dx.doi.org/10.1177/41.7.8515047.

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We describe a new technique for the morphological characterization of immunohistochemically labeled neuron populations. We demonstrate that it is possible to fill neurons iontophoretically with Lucifer Yellow (LY) in fixed slices of cat visual cortex after the respective cells have been identified by indirect immunofluorescence for the neural cell adhesion molecule N-CAM 180, with the VC1.1 antibody or with an antibody against glutamate dehydrogenase (GAD). Morphological analysis of the injected cells at the light and electron microscopic level revealed that the N-CAM 180-positive neurons shar
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Tuccari, G., F. Fedele, G. Giuffrè, C. J. Trombetta, and G. Barresi. "Immunohistochemical Demonstration of Metallothionein in Eyes with Choroidal Melanomas." European Journal of Ophthalmology 10, no. 4 (2000): 312–17. http://dx.doi.org/10.1177/112067210001000407.

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Purpose The since immunohistochemically detectable metallothionein (MT) overexpression has been described in a variety of human tumours, including skin melanomas, in relation to different stages of tumour development and progression. Materials and Methods We used a monoclonal antibody to investigate the distribution of MT in 18 formalin-fixed, paraffin-embedded, surgically enucleated eyes with choroidal melanomas, from 18 patients (8 male, 10 female; age range 30–83 years, mean 58.7). Clinico-pathological details and follow-up data (2–124 months, mean 36.1) were also available. MT immunoreacti
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Paromova, Yana, Nadejda Timofeeva, Elena Maslova, et al. "General Principles and Special Features of Histological and Immunohistochemical Methods of Bone Tissue Examination." Journal of Biomimetics, Biomaterials and Biomedical Engineering 46 (June 2020): 45–51. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.46.45.

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The detailed research and analysis of modern data on the problem of methods of histological and immunohistochemical researches of connective tissue, bone in particular, was conducted by us so as to evaluate the possibility of creating a single method of bone tissue immunohistochemical analysis.The description was given in detail to most widespread methods of preparation of connective, bone in particular, tissues for the research conduction during experiments connected with the study of bone tissue regeneration including implants with bioactive coverage. The present-day medical data reflects a
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Liskina, I. V., L. M. Zahaba, S. D. Kuzovkova, and O. O. Мельник. "COMPARATIVE ANALYSIS OF M. TUBERCULOSIS DETECTION BY DIFFERENT HISTOLOGICAL METHODS IN LUNG TISSUE WITH PULMONARY TUBERCULOMA WITH DIFFERENT ACTIVITY OF SPECIFIC INFLAMMATION." Medical Science of Ukraine (MSU) 13, no. 1-2 (2017): 26–32. http://dx.doi.org/10.32345/2664-4738.1-2.2017.04.

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Relevance. Modern histological diagnostics of tuberculosis with using of histobacterioscopy is based on the application of Ziehl-Neelsen method, fluorescent and immunohistochemical methods. And it is mainly used for extrapulmonary forms of tuberculosis. From a scientific and practical standpoint, it is advisable to use these methods in a combined.
 Objective. To conduct a comparative analysis of the detection of M. tuberculosis and their antigens by various histological methods in pulmonary tissue with tuberculoma at different activities of a specific inflammatory process.
 Materials
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Melnychuk, Maksym P. "DIAGNOSTIC UTILITY OF IMMUNOHISTOCHEMICAL EXPRESSION OF KI-67, P63 AND AMACR IN PROSTATE INTRAEPITHELIAL NEOPLASIA." Wiadomości Lekarskie 76, no. 2 (2023): 420–26. http://dx.doi.org/10.36740/wlek202302125.

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The aim: To determine malignant transformation and progression ability of high grade and low grade prostate intraepithelial neoplasia with the help of immunohistochemical method. Materials and methods: The results of examination of 93 patients with PIN (50 patients with high grade PIN and 43 patients with low grade PIN) were assessed comparatively using immunohistochemical markers. Semiquantitative method was used to evaluate !"-67, #63 and AMACR tissue expression with four grades from „+” to „++++” or from 1 to 4 points: ‘+’ – low reaction, ‘++’ – poor reaction, ‘+++’ – moderate reaction and
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Uragoh, K., K. Sueishi, T. Nakamura, and S. Iwanaga. "A novel immunohistochemical method for in vivo detection of endotoxin using horseshoe crab factor C." Journal of Histochemistry & Cytochemistry 36, no. 10 (1988): 1275–83. http://dx.doi.org/10.1177/36.10.3047230.

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We developed a novel immunohistochemical method for in vivo detection of endotoxin (LPS) localization in relation to the biologically active region, by use of factor C, an initiation factor in the Limulus clotting system which is mediated by LPS, as a specific affinoligand to LPS, and using rabbit anti-factor C IgG. The competitive inhibition of various LPS, lipid A, anti-LPS factor, or polymyxin B to factor C binding indicates that the immunohistochemical reaction is specific to LPS. Investigating the time course of LPS distribution during 6 hr after IV injection of 5 mg/kg to rats, the great
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Guillaud, M., J. B. Matthews, A. Harrison, C. MacAulay, and K. Skov. "A Novel Image Cytometric Method for Quantitation of Immunohistochemical Staining of Cytoplasmic Antigens." Analytical Cellular Pathology 14, no. 2 (1997): 87–99. http://dx.doi.org/10.1155/1997/457297.

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Evaluation of molecular markers by immunohistochemical labelling of tissue sections has traditionally been performed by qualitative assessment by trained pathologists. For those markers with a staining component present outside of the nucleus, there has been no image histometric method available to reliably and consistently define cell interfaces within the tissue. We present a new method of approximating cellular boundaries to define cellular regions within which quantitative measurements of staining intensity may be made. The method is based upon Voronoi tessellation of a defined region of i
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Allithy, Amal Nooredeen, Islam Mohamed Magdi Ammar, and Maisa Hashem Mohammed. "Diagnostic and Prognostic Values of PTEN Expression in Functional and Pathological Endometrial Biopsies." Asian Pacific Journal of Cancer Biology 7, no. 1 (2022): 21–27. http://dx.doi.org/10.31557/apjcb.2022.7.1.21-27.

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Background: Endometrial carcinoma is the most common gynecological malignancy in developed countries. About 80% of endometrial carcinomas are preceded by atypical endometrial hyperplasia. PTEN is a tumor suppressor gene involved in many vital intracellular biological processes. PTEN is altered in more than 45% of endometrial carcinomas. The aim of this study was to evaluate the value immunohistochemical expression of PTEN in normal, hyperplastic and neoplastic endometrial tissues. Methods: Ninety-two endometrial samples were enrolled in this study. They were classified into normal (n=6), hyper
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Oleynikova, N. A., O. A. Kharlova, N. V. Danilova, I. A. Mikhailov, and P. G. Malkov. "Levamisole usage for the block of intestinal alkaline phosphatase in immunohistochemical staining." CLINICAL AND EXPERIMENTAL MORPHOLOGY 9, no. 3 (2020): 74–79. http://dx.doi.org/10.31088/cem2020.9.3.74-79.

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Introduction. Immunohistochemical staining by an indirect method with a chromogenic label requires en-zymes, among which is alkaline phosphatase (AP). AP is also present in human tissues. It can break down the molecules of the immunohistochemical substrate, which leads to significant background staining. It is necessary to block endogenous enzymes prior to immunohistochemical staining to reduce this effect. One of the ways to block endogenous alkaline phosphatase is to use levamisole solutions. The aim of the study was to describe the technique of levamisole use to block the alkaline phosphata
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Ueda, Junji, Hiroshi Saito, Hiroaki Watanabe, and B. Mark Evers. "Novel and quantitative DNA dot-blotting method for assessment of in vivo proliferation." American Journal of Physiology-Gastrointestinal and Liver Physiology 288, no. 4 (2005): G842—G847. http://dx.doi.org/10.1152/ajpgi.00463.2004.

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Immunohistochemical assessment of 5-bromo-2-deoxyuridine (BrdU) in tissue sections is a widely used method to evaluate cell proliferation in vivo. However, this method requires time-consuming preparation of paraffin sections and laborious counting of BrdU-labeled nuclei on multiple sections. Here, we report the development of a rapid and reliable method to quantitate BrdU incorporation in intestinal and liver tissues using a dot-blot method. In vivo models of colon or liver proliferation were used to analyze the usefulness and reliability of this new method. Mice were killed after BrdU injecti
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Chumasov, E. I., E. S. Petrova, V. B. Samedov, E. A. Kolos, and D. E. Korzhevskii. "Immunohistochemical markers to study the digestive organs." CLINICAL AND EXPERIMENTAL MORPHOLOGY 11, no. 4 (2022): 70–84. http://dx.doi.org/10.31088/cem2022.11.4.70-84.

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Introduction. To select an appropriate and informative histological method is a very important step in morphological analysis of the digestive organs, one of them being immunohistochemistry. The aim of this paper was to highlight the most effective immunohistochemical markers in addition to the well-known markers used in pathological diagnosis. Materials and methods. We studied sections of the intestine and pancreas of Wistar rats (n=20) and fragments of the human colon obtained during resection surgeries (n=4). We described and analyzed the results of immunohistochemical studies with neuronal
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Salehi, Fateme, Kalman Kovacs, Michael D. Cusimano, et al. "Immunohistochemical expression of nestin in adenohypophysial vessels during development of pituitary infarction." Journal of Neurosurgery 108, no. 1 (2008): 118–23. http://dx.doi.org/10.3171/jns/2008/108/01/0118.

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Object The aim of this work was to investigate the immunohistochemical expression of nestin, a member of the intermediate filament family, in adenohypophysial vasculature during development and progression of pituitary infarction. Methods Forty-five nontumorous adenohypophyses and 34 pituitary adenomas of various types, all exhibiting acute or healing infarcts, were examined immunohistochemically using the streptavidin-biotin-peroxidase complex method. Results In both adenohypophyses and pituitary adenomas without infarction, nestin was expressed in only a few capillaries and endothelial cells
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Pramasari, Cristiani Nadya, Hadi Irawiraman, Endang Sawitri, Nisa Muthi’ah, Dewi Arsih Sulistiani, and Sarah Prinadira. "EXPRESSION OF CALRETININ IN AMELOBLASTOMA AND ODONTOGENIC CYST IN A.W. SJAHRANIE GENERAL HOSPITAL SAMARINDA." Dentino: Jurnal Kedokteran Gigi 9, no. 1 (2024): 105. http://dx.doi.org/10.20527/dentino.v9i1.18871.

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Background : Ameloblastoma and odontogenic cysts have similar clinical and radiographic features but different treatments. Diagnosis is established by histologic features, but sometimes this step have difficulty making appropriate assessment because epithelial cells can show various variations. Preoperative misdiagnosis may result in inefficient treatment. In such situations, immunohistochemistry (IHC) with the use of proper markers might be needed for the differentiation of these lesions. Purpose : This study aimed to assess calretinin expression in ameloblastoma and various odontogenic cysts
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