Relevant bibliographies by topics / Immunohistochemistry - Diagnosis

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Immunohistochemistry - Diagnosis'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 February 2022

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Journal articles on the topic "Immunohistochemistry - Diagnosis"

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Jensen, Marianne Lidang, Ole Nielsen, Preben Johansen, and Per Pr??torius Clausen. "Immunohistochemistry in Tumor Diagnosis." Applied Immunohistochemistry 5, no. 1 (1997): 35–44. http://dx.doi.org/10.1097/00022744-199703000-00006.

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Miettinen, Markku. "Immunohistochemistry in Tumour Diagnosis." Annals of Medicine 25, no. 3 (January 1993): 221–33. http://dx.doi.org/10.3109/07853899309147868.

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Hall, P. A. "Immunohistochemistry in lymphoma diagnosis." BMJ 293, no. 6561 (December 13, 1986): 1520–21. http://dx.doi.org/10.1136/bmj.293.6561.1520.

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Dunbar, Erin, and Anthony T. Yachnis. "Glioma Diagnosis: Immunohistochemistry and Beyond." Advances in Anatomic Pathology 17, no. 3 (May 2010): 187–201. http://dx.doi.org/10.1097/pap.0b013e3181d98cd9.

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van der Valk, P., H. Mullink, P. C. Huijgens, T. M. Tadema, W. Vos, and C. J. L. M. Meijer. "Immunohistochemistry in Bone Marrow Diagnosis." American Journal of Surgical Pathology 13, no. 2 (February 1989): 97–106. http://dx.doi.org/10.1097/00000478-198902000-00002.

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Chilosi, M., L. Montagna, A. Benedetti, F. Menestrina, G. Pizzolo, and Cattedra di Ematologia. "Immunohistochemistry in Bone Marrow Diagnosis." American Journal of Surgical Pathology 13, no. 12 (December 1989): 1069. http://dx.doi.org/10.1097/00000478-198912000-00011.

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Gonzalez, Sergio, and Christel Bolte. "Immunohistochemistry in Epidermolysis Bullosa Diagnosis." American Journal of Dermatopathology 26, no. 1 (February 2004): 84. http://dx.doi.org/10.1097/00000372-200402000-00016.

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Prieto, Victor G., and Christopher R. Shea. "Immunohistochemistry of Melanocytic Proliferations." Archives of Pathology & Laboratory Medicine 135, no. 7 (July 1, 2011): 853–59. http://dx.doi.org/10.5858/2009-0717-rar.1.

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Abstract Context.—Histologic analysis allows accurate classification of most melanocytic lesions as benign or malignant. Only in a minority of lesions is it necessary to use other techniques as an aid in the diagnosis. Among them, most authors recommend immunohistochemistry. Objective.—To describe how to apply immunohistochemistry to particular differential diagnoses and the potential pitfalls. Data Sources.—Personal experience and review of literature. Conclusions.—here is no single marker, or combination thereof, that establishes an unequivocal diagnosis of melanoma or nevus. Thus it is necessary to carefully analyze the pattern of expression (patchy versus diffuse) and localization (maturation) in the context of morphologic standard features.
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Deavers, Michael T. "Immunohistochemistry in Gynecologic Pathology." Archives of Pathology & Laboratory Medicine 132, no. 2 (February 1, 2008): 175–80. http://dx.doi.org/10.5858/2008-132-175-iigp.

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Abstract Although most diagnoses in gynecologic pathology are established through the examination of routine hematoxylin-eosin–stained slides, immunohistochemical studies can be of assistance in some cases. The following case studies are used to illustrate the value of some relatively new markers in the diagnosis of gynecologic neoplasms and to highlight potential diagnostic pitfalls.
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Roskams, Tania. "The role of immunohistochemistry in diagnosis." Clinics in Liver Disease 6, no. 2 (May 2002): 571–89. http://dx.doi.org/10.1016/s1089-3261(02)00012-0.

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Dissertations / Theses on the topic "Immunohistochemistry - Diagnosis"

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Kolivras, Athanassios. "Immunohistochemistry in the histopathological diagnosis of primary scalp alopecia." Doctoral thesis, Universite Libre de Bruxelles, 2016. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/238160.

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Primary scalp alopecia is classically divided into cicatricial (scarring) and non-cicatricial (non-scarring). Challenging cases are assessed with a scalp biopsy. The use of both horizontal and vertical sections (HoVert sections) has dramatically improved the accuracy of histopathological diagnosis. In this work, we have used immunostaining to address diagnostic difficulties, which persist despite all currently available tools. We performed an immunostain panel (CD3, CD4, CD8 and CD20) in order to distinguish pattern hair loss from alopecia aerate in cases which do not have the usual peribulbar lymphocytic infiltrate and showed that CD3+ T-lymphocytes within the empty fibrous follicular tracts favor a diagnosis of alopecia areata. We performed CD123 in order to distinguish lichen planopilaris from alopecia lupus erythematosus in cases with only a superficial lymphocytic infiltrate and an uninvolved interfollicular epidermis and showed that clusters of CD123+ plasmacytoid dendritic cells favor a diagnosis of lupus erythematosus. We performed cytokeratin 15 in order to assess whether the loss of the follicular bulge stem cells has diagnostic value in cicatricial alopecia and demonstrated that the loss of cytokeratin 15+ bulge stem cells is identified in lichen planopilaris, frontal fibrosing alopecia, and lupus erythematous, so cytokeratin 15 has no diagnostic value. We have attempted to integrate the new concepts and our findings into the traditional classifications of alopecia and proposed a new diagnostic algorithm. In conclusion, immunostaining combined with HoVert grossing advances the accuracy of histopathological diagnosis of primary scalp alopecia.
L’alopécie primitive du cuir chevelu est habituellement classée en cicatricielle et non-cicatricielle. Dans les cas difficiles, la biopsie du cuir chevelu peut aider au diagnostic. L’utilisation de coupes, à la fois verticales et horizontales sur le même spécimen (technique HoVert), a radicalement amélioré le diagnostic histopathologique. Dans ce travail, nous avons utilisé l’immunohistochimie pour évaluer les difficultés diagnostiques qui persistent malgré tous les outils actuels. Nous avons utilisé les CD3, CD4, CD8 et CD20 pour différencier l’alopécie androgénique de la pelade dépourvue de l’infiltrat lymphocytaire péribulbaire habituel et nous avons démontré que la présence de lymphocytes CD3+ dans les travées folliculaires fibreuses est en faveur de la pelade. Nous avons utilisé le CD123 pour différencier le lichen plan pilaire du lupus érythémateux alopécie avec infiltrat lymphocytaire superficiel et sans atteinte de l’épiderme interfolliculaire et nous avons démontré que la présence d’amas de cellules dendritiques plasmacytoïdes CD123+ est en faveur du lupus érythémateux. Nous avons utilisé la cytokératine 15 pour évaluer si la perte des cellules souches du bulge a une valeur diagnostique dans l’alopécie cicatricielle et nous avons démontré que cette perte s’observait de manière identique dans le lichen plan pilaire, l’alopécie frontale fibrosante comme dans le lupus érythémateux et n’avait donc aucune valeur diagnostique. Nous avons tenté d’intégrer les nouveaux concepts et nos données dans les classifications traditionnelles des alopécies et nous avons élaboré un nouvel algorithme diagnostique. L’association des immunomarquages avec la technique HoVert ouvre de nouvelles perspectives dans le diagnostic histopathologique des alopécies primaires du cuir chevelu.
Doctorat en Sciences médicales (Médecine)
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Ashton-Key, Margaret Rose. "New approaches to the diagnosis of malignant lymphomas." Thesis, University of Southampton, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242743.

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De, Silva Roxanne. "The use of collagen IV immunohistochemistry in the diagnosis of bullous pemphigoid." Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/25249.

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Background: Autoimmune bullous dermatoses present with overlapping clinical features that require histopathological correlation. Immunofluorescence is the most routinely used reliable investigation for diagnosis but requires specialised equipment and is technically sophisticated. Collagen IV immunohistochemistry is reported as a reliable test for the diagnosis of epidermolysis bullosa acquisita whereby It stains the roof of a subepidermal blister and would be expected on the floor in bullous pemphigoid. This technique could be performed as an easily accessible alternative to direct immunofluorescence and has been used anecdotally at our hospital. Aim: To investigate whether collagen IV immunohistochemistry can be used as a reliable histopathological confirmation of bullous pemphigoid. Methods: Two major investigations: 1. A systematic literature search was undertaken of all studies describing the use of collagen IV immunohistochemistry and those comparing it with immunofluorescence in the diagnosis of bullous pemphigoid. 2. A retrospective study of patients diagnosed with bullous pemphigoid over 12 years seen at Groote Schuur Hospital was performed. Patient records that had results for both direct immunofluorescence and collagen IV immunohistochemistry were selected. The positive percentage agreement was calculated. Results: 1. Two studies were found that investigated the use of collagen IV immunohistochemistry in bullous pemphigoid. All reported 33 (100%) cases demonstrated collagen IV at the floor of a subepidermal blister. Of these, 25/25 cases were in agreement with direct immunofluorescence and 7/8 with indirect immunofluorescence which were used as reference standard investigations. 2. In this study, collagen IV was positive in 96% (79/82) of cases and direct immunofluorescence was positive in 85% (72/82) of cases. A positive percentage agreement of 80.5% suggested a strongly positive test accordance. Limitations: 1. The literature search was limited to articles written in english only. 2. The retrospective design and the lack of controls without bullous pemphigoid made it impossible to calculate sensitivity and specificity as well as the kappa statistic. Conclusion: Collagen IV immunohistochemistry is a valid, simple and widely available test which demonstrates accordance with routinely used direct immunofluorescence in the confirmation of bullous pemphigoid. Through clinical and histomorphological correlation, it may be a useful test in resourcelimited settings without facilities for direct immunofluorescence. However, larger controlled studies are warranted to confirm this.
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Grant, John William. "Immunohistochemistry in the diagnosis and characterisation of neoplasms affecting the central nervous system." Thesis, University of Aberdeen, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278778.

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This work describes 5 groups of tumours seen in routine neuropathological practice in Southampton between 1980 and 1986. The clinical and light microscopic findings in a total of 44 tumours are described. A panel of monoclonal and polyclonal antibodies was used in immunohistochemical studies on each group, and this made important contributions to the diagnosis and characterisation of these tumours. Six primary central nervous system lymphomas were shown to be 5 follicle centre cell lymphomas (Kiel-classification) and 1 T-cell lymphoma. 15 lymphomas causing spinal cord compression were typed as 11 follicle centre cell lymphomas, 3 T-cell lymphomas and 1 lymphoblastic lymphoma. T-cell lymphomas appear to be more likely to be localised in the spine and to have a better prognosis. In all these tumours admixed reactive cell populations were also identified immunohistochemically. Ten primitive neuroectodermal tumours of the cerebrum were examined and immunohistochemistry assisted in their distinction from lymphoma and metastatic carcinoma. It also showed evidence of differentiation in apparently poorly differentiated parts of the tumours. Immunohistochemical studies facilitated the distinction of pleomorphic xanthoastrocytoma from a monstrocellular astrocytoma and a meningeal malignant fibrous histiocytoma. The recognition of this first entity has important prognostic implications. In 10 cerebellar haemangioblastomas a panel of antibodies was used to investigate the histogenesis of the stromal cell component of these tumours. Endothelial and stromal cells were found to be antigenically distinct and neurone specific enolase activity was found in the latter. The implications of this finding are discussed. The studies confirm the important and increasing role played by immunohistochemistry in our understanding of central nervous system neoplasia.
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Praciano, ClaudÃnia Costa. "Press imprint smear: um mÃtodo simples, rÃpido e de baixo custo no diagnÃstico de Leishmaniose cutÃnea." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13182.

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
A leishmaniose cutÃnea (LC) à a forma mais comum da leishmaniose, com 1 milhÃo de novos casos por ano. Apresenta um amplo espectro clÃnico, podendo ser confundida com outras doenÃas que acometem a pele. Desse modo, à de fundamental importÃncia o diagnÃstico rÃpido e preciso da doenÃa. O diagnÃstico parasitolÃgico à o mais utilizado na rotina laboratorial, entretanto o exame direto geralmente apresenta baixa sensibilidade. Em virtude disso, torna-se necessÃrio buscar tÃcnicas alternativas para o diagnÃstico das leishmanioses. Neste trabalho, comparou-se o teste do press imprint smear com o exame histolÃgico e a imuno-histoquÃmica, no diagnÃstico direto de leishmaniose cutÃnea. Pacientes com suspeita clinica de LC foram submetidos à coleta de duas biÃpsias da borda das lesÃes, com punch de 3 mm, para a realizaÃÃo dos testes: press imprint smear, exame histolÃgico e imuno-histoquÃmica. Este Ãltimo foi considerado o teste padrÃo ouro. O press imprint smear consistiu em esmagar o fragmento de biÃpsia entre duas lÃminas de vidro sob pressÃo, de tal modo que o extrato (suco) tissular se espalha na superfÃcie das duas lÃminas. Os esfregaÃos foram corados com Giemsa, para pesquisa de amastigotas. Outro fragmento foi fixado em formalina a 10%. Desta amostra, uma lÃmina foi utilizada no exame histolÃgico, corada com hematoxilina-eosina, e outra para o teste de imuno-histoquÃmica. Ao total, foram incluÃdos 78 pacientes com diagnÃstico clinico-epidemiolÃgico de LC. A tÃcnica do press imprint smear e o exame histolÃgico foram realizados na amostra de todos os pacientes. Entretanto, a imuno-histoquÃmica foi realizada em apenas 73 amostras. Setenta e dois (98,6%) foram positivos para Leishmania na imuno-histoquÃmica, com sensibilidade de 98,6%; o press imprint smear foi positivo em 48 pacientes (61,5%), com sensibilidade de 58,3%. No exame histolÃgico foram identificadas amastigotas em 35 pacientes (44,9%), apresentando sensibilidade de 45,8%. Os resultados mostraram que o press imprint smear apresentou maior sensibilidade do que o exame histolÃgico, para o diagnÃstico da leishmaniose cutÃnea. AlÃm disso, à uma tÃcnica de baixo custo e fÃcil execuÃÃo.
The cutaneous leishmaniasis (CL) is the most common form of leishmaniasis, with 1 million new cases per year. Presents a broad clinical spectrum, which may be confused with other diseases that affect the skin. Thus, it is of fundamental importance to rapid and accurate diagnosis of the disease. Parasitological diagnosis is the most used test in laboratory routine, however direct examination generally has low sensitivity. As a result, it becomes necessary to find alternative techniques for the diagnosis of leishmaniasis. This study compared the test press imprint smear with histological examination and immunohistochemistry, for the direct diagnosis of leishmaniasis. Patients with clinical suspicion of LC underwent a sampling from two samples of biopsies from the edge of the lesions, with a punch of 3 mm for the tests: press imprint smear, histological examination and immunohistochemistry. The latter was considered the gold standard test. The press imprint smear consisted in crushing the biopsy fragment between two glass plates under pressure so that the extract (juice) tissue spreads on the surface of the two blades. The smears were stained with Giemsa for the detection of amastigotes. Another fragment was fixed in 10% formalin. From this sample, a blade was used in histology, stained with hematoxylin-eosin, and one for the immunohistochemical test. In total, 78 patients were included with clinical and epidemiological diagnosis of LC. The press imprint smear technique and histologic examination were conducted on the sample of all patients. However, immunohistochemistry was performed in only 73 samples. Seventy-two (98,6%) were positive for leishmania in immunohistochemistry, with sensitivity of 98,6%; the press imprint smear was positive in 48 patients (61,5%), with sensitivity of 58,3%. In the histological examination were identified amastigotes in 35 patients (44,9%), with sensitivity of 45,8%. The press imprint smear was more sensitive than histological examination for the diagnosis of cutaneous leishmaniasis. In addition, a technique is inexpensive and easy to perform.
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Mazyambe, Margaret Kena. "Evaluating the specancer cell targeting peptides for applications in cancer diagnostics." University of the Western Cape, 2013. http://hdl.handle.net/11394/4266.

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>Magister Scientiae - MSc
Cancer is a disease most often associated with poor prognosis. During the development of the disease, cells acquire genetic mutations which result in changes in bio-molecules (DNA and protein), thus altering normal functioning of cells. These bio-molecules can thus serve as biomarkers for the diagnosis of cancer and can also facilitate the early detection of cancer. Antibodies labelled with organic fluorophores are typically used in immunohistochemistry techniques to screen cancerous tissue for the presence of biomarkers. More recently, researchers started to use cancer specific peptides (e.g LYP-1, RGD,) rather than antibodies for this purpose. Advantages of peptides include high affinity to their binding target, rapid accumulation at target sites and the ability to evade the immune system. Fluorescent nanocrystals or quantum dots are emerging as nanoparticles that can replace organic fluorophores. Several properties of quantum dots make these nanoparticles an ideal application in the detection of cancer related biomarkers. These include size tunable fluorescence emission, resistance to photobleaching as well as high quantum yields that result in bright emission of fluorescence. The aim of this research project was to investigate the specific binding of selected peptides to cancer cells using functionalized quantum dots. Since the cost of synthetic peptides are so high, the aim of this study was also to express these peptides in E.coli bacterial cells. Cancer targeting peptides were identified from literature and oligonucleotides with sequences encoding these peptides were designed. Four oligonucleotides encoding the peptides p6.1, p.L, MV and NL1.1 were successfully cloned using the pET21b plasmid vector. However, the peptides were not successfully expressed in E.coli. Cancer targeting peptides namely p.C, p.H, p.L, p6.1 and Frop-1 were chemically synthesized and obtained from GL biochem (Shanghai). These peptides were conjugated to quantum dots (Qdot 525) using 1-ethyl-3-(3-dimethylamino) carbodiimide HCl (EDC) chemistry. The peptidequantum dot conjugates were applied to cancer cells to achieve specific binding. The Kmst-6 noncancerous cell line served as a control. The binding of the peptide-quantum dot conjugates was analyzed using flow cytometry and fluorescence microscopy. The p.H peptide revealed the highest binding affinity to cancer cells as indicated by fluorescence intensity. This was followed by the p.C peptide which showed differential binding amongst the cancer cell lines. The Frop-1 peptide displayed the lowest binding affinity, while the binding affinity of the peptides to Kmst-6 cell lines was very low. This study demonstrated that the cancer targeting peptides used in this study bind to cancer cells and that the specificity with which these peptides bind to the cells depends on the cell types and the peptide
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Dadgar, Ashraf. "Methods for identification and diagnosis of amyloidosis." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7175.

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The amyloidoses are biochemically heterogeneous diseases with patholophysiologic deposits of various proteins. Amyloid deposits can occur either localized to one organ or tissue or as part of a systemic disease with deposits in many different tissue. The clinical course, prognosis and therapy are different for each type of amyloidosis and therefore a type specific diagnosis is demanded as early as possible. We describe a method for typing of the most common systemic amyloidoses based on Western blot analysis combined with specific

in- house antibodies, using subcutaneous fat biopsies. We found that the method is reliable and easy to perform and the tissue sample needed is obtained by minor surgery.

In the aortic intima amyloid deposits are often associated with atherosclerosis plaques. In our study we also investigated the prevalence of intimal amyloid from 10 patients age 58-94, amyloid deposits were present in 50% of the cases.


Amyloidos är ett sjukdomstillstånd där proteiner som normalt är lösliga i kroppen felveckas och formar långa olösliga fibriller som ansamlas i vävnader och organ såsom t.ex. hjärta, hjärna och lever. Det finns cirka 25 proteiner som kan ge upphov till amyloidos. Man kan skilja på två huvudgrupper av amyloidos, systemisk och lokaliserad. Vid lokal amyloidos kan inlagringar förekomma i specifika vävnader vid framför allt vissa åldersberoende sjukdomar som t.ex. Alzheimers sjukdom. Vid systemisk amyloidos förekommer inlagringar i praktiskt taget alla vävnader. Symtomatologin vid systemisk amyloidos är variabel och sjukdomsbilden kan vara svårtolkad men tidig och specifik diagnostik ger möjlighet till riktad terapi mot den bakomliggande sjukdomen. Syftet med denna studie var att utvärdera en Western blot metod som använts för typning av vanligaste formerna av systemisk amyloidos. De slutsatser som nåtts är att denna metod är snabbt, pålitligt och enkel att utföra. Diagnos erhölls med finnålsbiopsi av bukfettvävnad som är enkel, snabb och billig metod med liten risk för patienternas hälsa. Vi lyckades också med hjälp av immunhistokemisk infärgning titta på prevalens av amyloid i aortas intima.

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Kårsnäs, Andreas. "Image Analysis Methods and Tools for Digital Histopathology Applications Relevant to Breast Cancer Diagnosis." Doctoral thesis, Uppsala universitet, Avdelningen för visuell information och interaktion, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-219306.

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In 2012, more than 1.6 million new cases of breast cancer were diagnosed and about half a million women died of breast cancer. The incidence has increased in the developing world. The mortality, however, has decreased. This is thought to partly be the result of advances in diagnosis and treatment. Studying tissue samples from biopsies through a microscope is an important part of diagnosing breast cancer. Recent techniques include camera-equipped microscopes and whole slide scanning systems that allow for digital high-throughput scanning of tissue samples. The introduction of digital pathology has simplified parts of the analysis, but manual interpretation of tissue slides is still labor intensive and costly, and involves the risk for human errors and inconsistency. Digital image analysis has been proposed as an alternative approach that can assist the pathologist in making an accurate diagnosis by providing additional automatic, fast and reproducible analyses. This thesis addresses the automation of conventional analyses of tissue, stained for biomarkers specific for the diagnosis of breast cancer, with the purpose of complementing the role of the pathologist. In order to quantify biomarker expression, extraction and classification of sub-cellular structures are needed. This thesis presents a method that allows for robust and fast segmentation of cell nuclei meeting the need for methods that are accurate despite large biological variations and variations in staining. The method is inspired by sparse coding and is based on dictionaries of local image patches. It is implemented in a tool for quantifying biomarker expression of various sub-cellular structures in whole slide images. Also presented are two methods for classifying the sub-cellular localization of staining patterns, in an attempt to automate the validation of antibody specificity, an important task within the process of antibody generation.  In addition, this thesis explores methods for evaluation of multimodal data. Algorithms for registering consecutive tissue sections stained for different biomarkers are evaluated, both in terms of registration accuracy and deformation of local structures. A novel region-growing segmentation method for multimodal data is also presented. In conclusion, this thesis presents computerized image analysis methods and tools of potential value for digital pathology applications.
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Tran, Quoc Viet. "NEW INSIGHTS IN THE DIAGNOSIS AND MANAGEMENT OF HIRSCHSPRUNG’S DISEASE." Doctoral thesis, Universite Libre de Bruxelles, 2018. https://dipot.ulb.ac.be/dspace/bitstream/2013/263487/4/Thesis.pdf.

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Hirschsprung’s disease is a common pathology in pediatric surgery. Besides, long-term outcome of surgically-treated patients remains a crucial issue. The management of Hirschsprung’s disease has remarkably advanced over the years, but difficulties persist particularly in the developing countries (such as Vietnam), where essential diagnostic procedures, such as preoperative histopathological exploration techniques/ facilities (mainly for acetylcholinesterase staining), or adequate postoperative management and follow-up requirements are unavailable.We, therefore, contemplated to work-out a relevant histo-diagnostic approach to overcome these constraints that limit our diagnostic approaches, namely, in Vietnam, and we introduced a “less-demanding” diagnostic approach, namely calretinin immunohistochemical staining which is known to be adequate for formalin-fixed tissues (and thus not necessitating frozen section equipment). We thus used calretinin immunohistochemistry in a prospective study on a large cohort of Vietnamese HD cases. Results showed that rectal suction biopsy using calretinin immunohistochemistry provides an effective histopathological diagnostic tool that can replace AChE and provides a valuable evaluating approach for both preoperative and postoperative management.In addition, we also studied long-term outcome in operated patients and impact of postoperative morbidities on their quality of life. Indeed, a long-term multidisciplinary management with dedicated procedures such as anorectal manometry is essentially required for patients with severe defecation disorders.
Doctorat en Sciences biomédicales et pharmaceutiques (Médecine)
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Ferreira, Rita de Cassia. "Contribuição da analise de textura do nucleo celular para o diagnostico diferencial de lesões foliculares da tireoide : comparação com marcadores imunoistoquimicos." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311564.

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Orientadores: Patricia Sabino de Matos, Konradin Metze
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: O diagnóstico diferencial entre algumas lesões da tireóide é, muitas vezes, difícil de ser determinado, mesmo diante de diversas amostras de tecido. O propósito deste estudo foi o de investigar até que ponto a análise de textura da cromatina em cortes histológicos de rotina contribui para o diagnóstico diferencial entre lesões foliculares da glândula tireóide. Entraram no estudo cortes histológicos corados com hematoxilina-eosina de 12 nódulos adenomatosos hiperplásicos (NA), 18 adenomas foliculares (AF), 24 carcinomas foliculares mínimamente invasores (CFMI) e 22 variantes foliculares do carcinoma papilífero (VFCP). As amostras de tecido utilizadas foram analisadas morfologicamente por meio de estudo histológico e estudo imunoistoquímico pela expressão de diferentes proteínas ou marcadores. Os marcadores utilizados foram HBME-1, CK-19 e galectina-3. Em cada caso 100 núcleos foram aleatoriamente escolhidos, digitalizados e segmentados. A estrutura dos núcleos foi descrita por morfometria, densitometria e por variáveis derivadas da matriz de co-ocorrência. Sexo, idade do paciente e metástases foram informações pesquisadas. Os resultados da análise de imagem computadorizada foram comparados com aqueles obtidos por meio de estudo imunoistoquímico, na distinção das lesões foliculares benignas e malignas da tireóide. Os dados foram comparados por análise de variância (nível de significância p<0,05). As amostras sugerem que o HBME-1 foi um marcador mais sensível quanto à malignidade. Entretanto, o uso dos três marcadores, num painel, pode ser útil em casos específicos, como por exemplo, na diferenciação de lesões foliculares, com referência especial à variante folicular do carcinoma papilífero. Dados descritos como fatores prognósticos, nas lesões foliculares malignas, não se mostraram significantes para discriminar o carcinoma folicular da variante folicular do carcinoma papilífero. As variáveis de morfometria não apresentaram grande valor discriminante, exceto, pelo P5FF4 (quinto percentil do fator de forma) que estabeleceu diferenças significantes entre AF e VFCP e entre CFMI e VFCP. Variáveis densitométricas demonstraram diferenças significantes entre NA e AF, NA e CFMI, AF e VFCP, AF e CFMI e entre CFMI e VFCP. Variáveis derivadas da matriz de co-ocorrência demonstraram diferenças entre os subgrupos, mas somente o P5Energy (quinto percentil da variável ?energia?), demonstrou diferença entre AF e CFMI. Ainda a variável r2Mink (grau de aproximação da dimensão fractal de Minkowski) diferenciou lesões benignas de malignas (NA e CFMI, NA e VFCP, AF e CFMI, AF e VFCP). A análise de textura pôde auxiliar na discriminação entre lesões benignas e malignas da tireóide principalmente quando se tratava da discriminação entre NA ou AF e VFCP, na qual a análise histológica, baseada em características nucleares pode trazer grandes dificuldades. Por outro lado, o diagnóstico entre AF e CFMI é difícil e não se baseia em características nucleares, mas no achado de invasão capsular ou vascular. Entretanto, nestes casos, as variáveis 'energia' e r2Mink, contribuíram para o diagnóstico diferencial. Portanto, a análise de imagem computadorizada pode ser considerada como uma ferramenta que auxilia no diagnóstico diferencial de lesões foliculares da tireóide, somando-se à interpretação morfológica e imunoistoquímica
Abstract: The differential diagnosis between some thyroid lesions is often difficult to determine, even with permanent sections. The purpose of this study was to find out if chromatin texture analysis contributes to the differential diagnosis of thyroid follicular lesions. We selected slides stained with H-E (hematoxilin-eosin) of 12 hyperplastic adenomatous nodules (AN), 18 follicular adenomas (FA), 24 minimally invasive follicular carcinomas (MIFC) and 22 follicular variants of papillary carcinomas (FVPC). A total of 100 nuclei of each case were digitalized and segmented. The utility of a combination of immunostaining including galectin-3, HBME-1 and CK-19 in the routine differentiation of the histologies of thyroid malignancies was evaluated. The nuclei structure was described by morfometry, densitometry and by variables derived by co-occurrence matrix. Sex, age of the patient and metastasis were clinical and morphologic parameters searched in this study. These data, described as prognostic factors for follicular malignant tumors, were compared by variance analysis (significance value p<0,05) and did not differentiate significantly among the groups. The best marker, in terms of sensitivity and specificity, was HBME-1. The combination CK-19, HBME-1 and galectin-3, in a panel, may be useful in specific cases, for example, for differentiate folicullar lesions, with special reference for folicullar variant of papillary carcinoma. Morphometric variables did not show significant discriminant value, except, the P5FF4 (5th percentile of the form factor) which demonstrated significant differences between FA and FVPC and between MIFC and FVPC. Densitometric variables demonstrated significant differences between AN and FA, AN and MIFC, FA and FVPC, FA and MIFC and even between MIFC and FVPC. The variables derived from co-occurrence matrix demonstrated differences among the sub- groups, but only P5 Energy (fifth percent of energy variable) showed significant differences between FA and MIFC. Also, the variable r2Mink (Goodnesss of fit of the Minkowski - fractal dimension) was able to differentiate benign and malignant lesions such as: AN and MIFC, AN and FVPC, FA and MIFC, FA and FVPC. Texture analysis may help to differentiate benign and malignant follicular lesions of the thyroid, specially when one is trying to differentiate AN or FA and FVPC. In this situation the histological analysis based on nuclear characteristics may not be definitive; owing to the fact that all the nuclear criteria may not be always present in most cases. On the other hand, the differential diagnosis of FA and MIFC is difficult and it is not based on nuclear characteristics, but on the finding of capsular or vascular invasion. However, in these cases, the variables energy and r2Mink contributed to the differential diagnosis. Therefore, the computerized image analysis may be considered as a tool that helps the differential diagnosis of follicular thyroid lesions, added to the morphological and immunohistochemical analysis
Doutorado
Anatomia Patologica
Doutor em Ciências Médicas
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Books on the topic "Immunohistochemistry - Diagnosis"

1

M, Elias Jules, ed. Immunohistopathology: A practical approach to diagnosis. Chicago: ASCP Press, 1990.

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Chu, Peiguo. Modern immunohistochemistry. Cambridge: Cambridge University Press, 2009.

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Flow cytometry, immunohistochemistry, and molecular genetics for hematologic neoplasms. 2nd ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins, 2012.

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Leong, Anthony S. Y. Quantitative immunohistology: Problems and solutions. Hauppauge, N.Y: Nova Science Publishers, 2010.

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R, Morales Azorides, ed. Immunoperoxidase techniques: A practical approach to tumor diagnosis. Chicago: American Society of Clinical Pathologists Press, 1986.

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1952-, Wick Mark R., and Swanson Paul E. 1957-, eds. Immunohistology and electron microscopy of anaplastic and pleomorphic tumors. Cambridge: Cambridge University Press, 1997.

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Jasani, Bharat. Immunocytochemistry in diagnostic histopathology. Edinburgh: Churchill Livingstone, 1993.

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W, Schmid Kurt, ed. Immunocytochemistry in diagnostic histopathology. Edinburgh: Churchill Livingstone, 1993.

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Tuffaha, Muin S. A., Hans Guski, and Glen Kristiansen. Immunohistochemistry in Tumor Diagnostics. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-53577-7.

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Yazdi, Hossein M. Diagnostic immunocytochemistry and electron microscopy. New York: Igaku-Shoin, 1992.

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Book chapters on the topic "Immunohistochemistry - Diagnosis"

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Subtil, Antonio. "Basic Immunohistochemistry Panels." In Diagnosis of Cutaneous Lymphoid Infiltrates, 71–74. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-11654-5_15.

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Scully, Robert E. "Immunohistochemistry of Ovarian Tumors." In Immunocytochemistry in Tumor Diagnosis, 293–320. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2615-1_18.

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Tuffaha, Muin S. A., Hans Guski, and Glen Kristiansen. "Immunohistochemical Markers for the Diagnosis of Epithelial Tumors." In Immunohistochemistry in Tumor Diagnostics, 13–27. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-53577-7_2.

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Tuffaha, Muin S. A., Hans Guski, and Glen Kristiansen. "Recommendations for the Utility of Immunohistochemistry in Tumor Diagnosis." In Immunohistochemistry in Tumor Diagnostics, 257–58. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-53577-7_32.

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Tubbs, Raymond R. "Immunohistochemistry of Lymphomas in Frozen Tissue Sections." In Immunocytochemistry in Tumor Diagnosis, 116–40. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2615-1_8.

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Chan, C. C., and D. F. Shen. "Newer Methodologies in Immunohistochemistry and Diagnosis." In Uveitis Update, 1–13. Basel: KARGER, 1999. http://dx.doi.org/10.1159/000060753.

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Tuffaha, Muin S. A., Hans Guski, and Glen Kristiansen. "Markers to Assist the Diagnosis of Dysplasia and Malignant Transformation." In Immunohistochemistry in Tumor Diagnostics, 253–55. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-53577-7_31.

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Pantanowitz, Liron, Gabriel Caponetti, and Bruce J. Dezube. "The Immunohistochemistry of Kaposi’s Sarcoma." In Methods of Cancer Diagnosis, Therapy, and Prognosis, 405–31. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-2918-8_33.

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Rubinstein, L. J. "Immunohistochemistry in Neuro-Oncology: Markers or Signposts?" In Brain Oncology Biology, diagnosis and therapy, 31–34. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3347-7_6.

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Ponnusamy, Moorthy P., Ajay P. Singh, Subodh M. Lele, and Surinder K. Batra. "Ovarian Carcinoma: Diagnostic Immunohistochemistry of MUCIN4 (MUC4)." In Methods of Cancer Diagnosis, Therapy, and Prognosis, 13–21. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-2918-8_2.

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Conference papers on the topic "Immunohistochemistry - Diagnosis"

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Ngo, Carine, Claire Daniel, Sy Duong-Quy, Olivier Brugière, and Aurélie Cazes. "Utility of C4d by immunohistochemistry for the diagnosis of antibody-mediated rejection in lung transplantation." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa4636.

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Noureddine, Kouther, Paul Gallagher, Martial Guillaud, and Calum MacAulay. "Abstract PO-043: Combining multiplexed immunohistochemistry and deep learning to spatially map the tumor microenvironment." In Abstracts: AACR Virtual Special Conference on Artificial Intelligence, Diagnosis, and Imaging; January 13-14, 2021. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1557-3265.adi21-po-043.

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Zhao, Jin-Hui, Zhan-Cui Chen, Hong Zhang, Shi-Cai Hou, and Gang-Ping Wang. "Application of Immunohistochemistry: CD10, h-Caldesmon, SMA, Ki-67, ER and PR in Diagnosis and Differential Diagnosis of Endometrial Stromal Sarcomas." In 2015 International Conference on Medicine and Biopharmaceutical. WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789814719810_0055.

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Sajja, Sujith V., Matthew P. Galloway, Farhad Ghoddoussi, T. Dhananjeyan, Andrea Kespel, and Pamela VandeVord. "Possible Mechanism of Blast-Induced Neuronal Damage in Hippocampus May Explain Cognitive Deficits." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19545.

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Traumatic brain injury due to blast exposure is quickly becoming the most frequently seen injury in today’s battlefields. Alterations in cognitive function, such as attention, memory, language and problem solving skills appear to occur as a result of blast-induced TBI. Furthermore, behavioral symptoms such as mood changes, depression, anxiety, impulsiveness and emotional outbursts are associated with blast-induced TBI (Okie et al, 2005). Observed overlaps between symptoms of post-traumatic stress disorder (PTSD) and TBI confound the differential diagnosis. Thus, soldiers with blast-induced TBI may be substantially under-diagnosed after exposure to blast waves. Animal models of blast-induced TBI are underdeveloped and there is a vital need for blast exposure biomarkers to help effectively diagnosis blast-induced TBI. In this study, we have investigated the mechanisms that underlie cognitive impairment of blast-induced neurotrauma. We have studied the cascade of neurochemical changes within the hippocampus of blast-exposed animals using 1H-Magnetic Resonance Spectroscopy (1HMRS). Furthermore, we examined changes in TBI protein markers using Western blotting and immunohistochemistry. Results suggest that exposure to blast waves has a significant effect on the hippocampus.
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Owens, VL, JM Boland, SM Jenkins, JL Donovan, AL Feldman, MC Aubry, and ES Yi. "PAX-5 Immunohistochemistry for Differential Diagnosis of Small Cell and Squamous Cell Carcinomas of the Lung." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a1105.

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Cipullo, I., P. Modaffari, R. Ponzone, F. Maggiorotto, P. Campisi, G. De Rosa, D. Balmativola, et al. "EP1075 Immunohistochemistry improves specificity in the diagnosis of preneoplastic fallopian tube lesions of BRCA mutation carriers." In ESGO Annual Meeting Abstracts. BMJ Publishing Group Ltd, 2019. http://dx.doi.org/10.1136/ijgc-2019-esgo.1117.

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Gilles, Erard, Jacques Bosq, Laura Caplier, Alice Bexon, Alexander Zukiwski, and Jacques Bonneterre. "Abstract 5567: Impact of progesterone receptor (PR) isotype-specific immunohistochemistry (IHC) on the diagnosis of triple negative breast cancer (BC)." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-5567.

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Peljto, Mirza, Joseph Krueger, Erik Hagendorn, David Young, Steve Potts, Quan Nguyen, Botoul Maqsodi, Takuro Yaoi, and Gary McMaster. "Abstract 44: Tissue image analysis tool to quantify HER2 RNA and protein expression by colorimetricin situhybridization and immunohistochemistry for breast cancer diagnosis." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-44.

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Si, Stephanie J., David Barrett, and Gerald Wertheim. "Abstract 454: Uniform TIM 3 and galectin 9 positivity on immunohistochemistry staining of tumor specimen at diagnosis in pediatric patients with Ewing sarcoma." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-454.

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Terata, Kaori, Hiroshi Nanjo, Eriko Takahashi, Ryuta Nakamura, Yoichi Akagami, Ayuko Yamaguchi, Misako Yatsuyanagi, Chiaki Kudo, and Yoshihiro Minamiya. "Abstract PS1-04: Utility of rapid- immunohistochemistry using an alternating current electric field for intraoperative diagnosis of sentinel lymph nodes in breast cancer." In Abstracts: 2020 San Antonio Breast Cancer Virtual Symposium; December 8-11, 2020; San Antonio, Texas. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.sabcs20-ps1-04.

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