Relevant bibliographies by topics / Immunological investigation

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Immunological investigation'

Author: Grafiati
Published: 4 June 2021
Last updated: 7 February 2022

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Journal articles on the topic "Immunological investigation"

1

Oʼleary, Colin, and Hugh J. Willison. "Immunological investigation." Current Opinion in Neurology 8, no. 5 (October 1995): 349–53. http://dx.doi.org/10.1097/00019052-199510000-00004.

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Hayward, A. R. "Laboratory Investigation of Immunological Disorders." Immunology Today 7, no. 7-8 (July 1986): 246. http://dx.doi.org/10.1016/0167-5699(86)90114-3.

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Meléndez-Vásquez, Carmen, Jane Redford, P. P. Choudhary, Ian A. Gray, Philip Maitland, Norman A. Gregson, Kenneth J. Smith, and Richard A. C. Hughes. "Immunological investigation of chronic inflammatory demyelinating polyradiculoneuropathy." Journal of Neuroimmunology 73, no. 1-2 (March 1997): 124–34. http://dx.doi.org/10.1016/s0165-5728(96)00189-0.

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Guidi, A., L. Castigliego, S. Preziuso, P. Gaspari, D. Gianfaldoni, and G. Del Bono. "Immunological and Histochemical Investigation of Darkened Turkey Muscles." Veterinary Research Communications 27 (2003): 687–90. http://dx.doi.org/10.1023/b:verc.0000014248.41842.08.

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Wu, Chih-Te, Paul A. Davis, VelImir A. Luketic, and M. Eric Gershwin. "A Review of the Physiological and Immunological Functions of Biliary Epithelial Cells: Targets for Primary Biliary Cirrhosis, Primary Sclerosing Cholangitis and Drug-induced Ductopenias." Clinical and Developmental Immunology 11, no. 3-4 (2004): 205–13. http://dx.doi.org/10.1080/17402520400004177.

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Our understanding of biliary epithelial cells (BEC) in physiobiology and immunology has steadily expanded. BEC transports IgA as well as IgM into bile, synthesizes and secretes various chemokines, cytokines, and expresses adhesion molecules involved in cell interaction and signal transduction. These then suggest a myriad of potential roles for BEC in defense from invading microorganisms as well as the pathogenesis of diverse immunologically driven diseases such as primary biliary cirrhosis (PBC), graft-versus-host disease, and primary sclerosing cholangitis (PSC). Despite the progress, there still remain many areas of BEC biology that require further investigation. Most importantly, it remains to be clarified that the extent to which the immunologic activities observed in BEC represent a BEC response to tissue injury or whether BEC themselves are the active participants in the pathogenesis of various cholestatic immunological diseases, including PBC and PSC.
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Berki, Alexandra Réka, Arany Attila Csaba, Jámbor Ilona, Szabó Krisztina, Mile Marianna, Makra Gabriella, Csiki Zoltán, Balogh László, and Papp Gábor. "Investigation on the Immunological Effects of Regular Physical Activity." STADIUM - Hungarian Journal of Sport Sciences 2, no. 1 (August 26, 2019): 1–15. http://dx.doi.org/10.36439/hjsc/2019/1/2929.

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Introduction: Effects of physical activity on immune functions may largely depend on the type of activity, its intensity and duration. However, little information is available regarding the immunological effects of sporting activities in older ages. The aim of our study was to examine the changes in a wide spectrum of lymphocyte subtypes after a period of regular workout among healthy women of different ages. Methods: In our study, we enrolled 16 elderly women (between ages of 60-75 years) formerly not engaged in regular physical activity, who took part in lightweight conditioning gymnastic exercise program. Eight young athletes and 26 young adults with sedentary lifestyle served as controls. The percentages of peripheral NK, NKT cells, T and B lymphocyte subtypes were determined by flow cytometry based on the staining of extracellular markers and intracellular cytokines. Results: Lymphocyte subgroups did not differ between the groups of young athletes and young adults with sedentary lifestyle. However, in elderly women, levels of NKT cells were lower, while ratios of T helper / T cytotoxic cells were higher compared to younger individuals. At the end of exercise program, in elderly women, percentages of naive B cells decreased, while levels of switched-memory B cells increased. Furthermore, proportions of Th2 cells increased, while levels of immunosuppressive CD4+CD127-CD25bright Treg cells decreased as the result of regular exercise. Conclusion: Differences observed after lightweight exercise programs reflect a presumably enhanced immunoreactivity and increased ability for immune responses in older ages.
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Berki, Alexandra Réka, Attila Csaba Arany, Ilona Jámbor, Krisztina Szabó, Marianna Mile, Gabriella Makra, Zoltán Csiki, László Balogh, and Gábor Papp. "Investigation on the Immunological Effects of Regular Physical Activity." Stadium - Hungarian Journal of Sport Sciences 2, no. 1 (January 11, 2021): 1–15. http://dx.doi.org/10.36439/shjs/2019/1/2929.

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Introduction: Effects of physical activity on immune functions may largely depend on the type of activity, its intensity and duration. However, little information is available regarding the immunological effects of sporting activities in older ages. The aim of our study was to examine the changes in a wide spectrum of lymphocyte subtypes after a period of regular workout among healthy women of different ages. Methods: In our study, we enrolled 16 elderly women (between ages of 60-75 years) formerly not engaged in regular physical activity, who took part in lightweight conditioning gymnastic exercise program. Eight young athletes and 26 young adults with sedentary lifestyle served as controls. The percentages of peripheral NK, NKT cells, T and B lymphocyte subtypes were determined by flow cytometry based on the staining of extracellular markers and intracellular cytokines. Results: Lymphocyte subgroups did not differ between the groups of young athletes and young adults with sedentary lifestyle. However, in elderly women, levels of NKT cells were lower, while ratios of T helper / T cytotoxic cells were higher compared to younger individuals. At the end of exercise program, in elderly women, percentages of naive B cells decreased, while levels of switched-memory B cells increased. Furthermore, proportions of Th2 cells increased, while levels of immunosuppressive CD4+CD127-CD25bright Treg cells decreased as the result of regular exercise. Conclusion: Differences observed after lightweight exercise programs reflect a presumably enhanced immunoreactivity and increased ability for immune responses in older ages.
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Alexandra Réka, Berki, Attila Csaba Arany, Ilona Jámbor, Krisztina Szabó, Marianna Mile, Gabriella Makra, Zoltán Csiki, László Balogh, and Gábor Papp. "Investigation on the Immunological Effects of Regular Physical Activity." STADIUM - Hungarian Journal of Sport Sciences 2, no. 1 (August 26, 2019): 1–15. http://dx.doi.org/10.36439/sjsc.v2i1.2929.

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Introduction: Effects of physical activity on immune functions may largely depend on the type of activity, its intensity and duration. However, little information is available regarding the immunological effects of sporting activities in older ages. The aim of our study was to examine the changes in a wide spectrum of lymphocyte subtypes after a period of regular workout among healthy women of different ages. Methods: In our study, we enrolled 16 elderly women (between ages of 60-75 years) formerly not engaged in regular physical activity, who took part in lightweight conditioning gymnastic exercise program. Eight young athletes and 26 young adults with sedentary lifestyle served as controls. The percentages of peripheral NK, NKT cells, T and B lymphocyte subtypes were determined by flow cytometry based on the staining of extracellular markers and intracellular cytokines. Results: Lymphocyte subgroups did not differ between the groups of young athletes and young adults with sedentary lifestyle. However, in elderly women, levels of NKT cells were lower, while ratios of T helper / T cytotoxic cells were higher compared to younger individuals. At the end of exercise program, in elderly women, percentages of naive B cells decreased, while levels of switched-memory B cells increased. Furthermore, proportions of Th2 cells increased, while levels of immunosuppressive CD4+CD127-CD25bright Treg cells decreased as the result of regular exercise. Conclusion: Differences observed after lightweight exercise programs reflect a presumably enhanced immunoreactivity and increased ability for immune responses in older ages.
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Alexandra Réka, Berki, Attila Csaba Arany, Ilona Jámbor, Krisztina Szabó, Marianna Mile, Gabriella Makra, Zoltán Csiki, László Balogh, and Gábor Papp. "Investigation on the Immunological Effects of Regular Physical Activity." Stadium - Hungarian Journal of Sport Sciences 2, no. 1 (August 26, 2019): 1–15. http://dx.doi.org/10.36439/sjsc/2019/1/2929.

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Abstract:
Introduction: Effects of physical activity on immune functions may largely depend on the type of activity, its intensity and duration. However, little information is available regarding the immunological effects of sporting activities in older ages. The aim of our study was to examine the changes in a wide spectrum of lymphocyte subtypes after a period of regular workout among healthy women of different ages. Methods: In our study, we enrolled 16 elderly women (between ages of 60-75 years) formerly not engaged in regular physical activity, who took part in lightweight conditioning gymnastic exercise program. Eight young athletes and 26 young adults with sedentary lifestyle served as controls. The percentages of peripheral NK, NKT cells, T and B lymphocyte subtypes were determined by flow cytometry based on the staining of extracellular markers and intracellular cytokines. Results: Lymphocyte subgroups did not differ between the groups of young athletes and young adults with sedentary lifestyle. However, in elderly women, levels of NKT cells were lower, while ratios of T helper / T cytotoxic cells were higher compared to younger individuals. At the end of exercise program, in elderly women, percentages of naive B cells decreased, while levels of switched-memory B cells increased. Furthermore, proportions of Th2 cells increased, while levels of immunosuppressive CD4+CD127-CD25bright Treg cells decreased as the result of regular exercise. Conclusion: Differences observed after lightweight exercise programs reflect a presumably enhanced immunoreactivity and increased ability for immune responses in older ages.
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Azovskaja, T. "Immunological investigation of acute lymphoblastic and myeloblastic leukemia variantes." Immunology Letters 56, no. 1-3 (May 1997): 452. http://dx.doi.org/10.1016/s0165-2478(97)88678-6.

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Dissertations / Theses on the topic "Immunological investigation"

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Souberbielle, Bernard. "Immunological investigation in multiple sclerosis." Thesis, University of St Andrews, 1993. http://hdl.handle.net/10023/13440.

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In this thesis, immunological parameters in multiple sclerosis patients (MS) have been examined and techniques contributing to the study of these aspects have been developed. The epidemiology of MS suggests that both environmental and genetic factors contribute to the disease process. According to a widespread theory, an autoimmune reaction leading to demyelination could be induced, in genetically determined individuals, by an infectious agent (s) e.g a paramyxovirus or a retrovirus. In the first part of the thesis, the oligoclonal immunoglobulin (Igs) in the CSF of MS patients in relation to the paramyxovirus SV5 were studied. This was to complement an initial observation made in the department that a significant proportion of cerebrospinal fluids (CSF) could have oligoclonal bands directly acting against antigens (Ags) of this virus. In the second part of the thesis, techniques were developed for the analysis of proliferative capacity of peripheral lymphocytes of MS and control patients using possible MS autoantigens viz myelin and brain vessels. The humoral response against these Ags was also assessed by immunoblotting using MS, neurological and normal control patients. In parallel and in the view to obtaining antigens for the immunological studies, techniques for the purification and characterisation of human brain vessels from post mortem brains were assessed and attempts to culture human brain endothelial cell lines were made.
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Budd, Timothy Charles. "An immunological investigation of neuromuscular transmission in insects." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334782.

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Boag, Alisdair Matthew. "An immunological and genetic investigation of canine hypoadrenocorticism (Addison's Disease)." Thesis, Royal Veterinary College (University of London), 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.618317.

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Sloper, K. S. "An immunological investigation into the aetiology of atopic eczema in children." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233526.

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O'Sullivan, Colette. "Epithelial mucins associated with breast carcinoma : purification, characterisation and immunological investigation." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290401.

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Oughterson, Susan Michelle. "Fish calcitonins and ultimobranchial gland - a histological, immunological and physiological investigation." Thesis, University of Salford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315453.

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Baker, Robert. "Investigation of glucocorticoid and local immunological function in tuberculosis and inflammation." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249300.

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Cooper, Philip. "Onchocerciasis in Ecuador : a cellular immunological and epidemiological investigation of chorioretinopathy." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338137.

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Broadbent, Andrew James. "Chlamydia trachomatis : quantification, immunological investigation and co-infection with HIV-1." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/5929.

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Three aspects of Chlamydia trachomatis translational research were explored in this thesis. Firstly, as over 75% of patients with LGV are also HIV-1 sero-positive, a cell-culture model was established to investigate whether HIV-1 altered the replication of C. trachomatis serovar L2 in vitro. Inclusion size was significantly increased in the presence of HIV-1, but there was no significant alteration in chlamydial growth kinetics, infectivity, morphology, or transcription of 16sRNA, ompA or euo, suggesting that viral co-infection did not induce chlamydial persistence. It is, therefore, unlikely that the association of HIV-1 and LGV in vivo is due to an impact of HIV-1 on chlamydial replication in co-infected cells. Secondly, as there is no information on the chlamydial load shed by individuals with a rectal C. trachomatis infection, a qPCR assay was developed and used to determine the number of C. trachomatis organisms per rectal swab in NAAT-positive patients. The geometric mean chlamydial load was 5.0 x 105 organisms per swab (Standard Deviation, 152) and load was associated with proctitis, but not symptoms or HIV-1 infection. Asymptomatic individuals shed as much C. trachomatis as patients with rectal symptoms and might maintain transmission in the community. Finally, an ex vivo IFN-γ ELISpot assay was developed to characterise human cellular immune responses to the C. trachomatis-specific protein, Pgp3. T-cell epitopes were found along the length of the protein, but the magnitude of the immune responses was low. The Pgp3- induced IFN-γ response correlated with C. trachomatis exposure and was dynamic, decreasing after effective treatment. These observations suggest that Pgp3- induced IFN-γ may be useful as a biomarker for current infection, although the sensitivity and specificity of the ELISpot assay need improvement.
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Freedman, A. R. "Investigation of the immunological mechanisms involved in the pathogenesis of coeliac disease." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599204.

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Books on the topic "Immunological investigation"

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Bruton, Rachel Kathlyn. An immunological and biochemical investigation of some cental metabotropic glutamate receptors (mGluRs). Birmingham: University of Birmingham, 1996.

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Oughterson, Susan Michelle. Fish calcitonins and the ultimobranchial gland: A histological, immunological and physiological investigation. Salford: University of Salford, 1992.

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Watson, J. Graham. Handbook of immunological investigations in children. London: Wright, 1990.

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Nichols, Eve K. Expanding access to investigational therapies for HIV infection and AIDS: March 12-13, 1990, conference summary. Washington, D.C: National Academy Press, 1991.

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Office, General Accounting. Gulf war illnesses: Procedural and reporting improvements are needed in DOD's investigative processes : report to the Honorable Lane Evans, Ranking Minority Member, Committee on Veterans Affairs, House of Representatives. Washington, D.C. (P.O. Box 37050, Washington, D.C. 20013): The Office, 1999.

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Sheila, Davey, World Health Organization, UNICEF, and World Bank, eds. State of the world's vaccines and immunization. 3rd ed. Geneva: World Health Organization, 2009.

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Committee on the Use of Animals in Research (U.S.), National Academy of Sciences (U.S.), and Institute of Medicine (U.S.), eds. Science, medicine, and animals. Washington, D.C: National Academy Press, 1991.

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Berden, Jo H. M., and Jack F. M. Wetzels. Immunological investigation of the patient with renal disease. Edited by Christopher G. Winearls. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199592548.003.0017.

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Laboratory techniques (electrophoresis, indirect immunofluorescence, ELISA, and immunoblotting) required for immunological investigation of the patient with renal disease are described. Renal disease-related aspects of immunoglobulins (immunoglobulin A, paraproteins, cryoglobulins), complement, antinuclear antibodies, anti-C1q antibodies, antineutrophil cytoplasmic antibodies, anti-glomerular basement membrane antibodies, antipodocyte antibodies, antiphospholipid antibodies, and antimicrobial responses (streptococci, hepatitis C, hepatitis B) are reviewed. Laboratory assays which evaluate the immune response, in particular the identification of (auto)-antibodies are valuable tools in establishing a diagnosis and/or monitoring of the activity of the disease. Guidelines are given for immunological studies in patients with specific renal syndromes including nephrotic syndrome, rapidly progressive glomerulonephritis, systemic lupus erythematosus, and thrombotic microangiopathy.
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Nithi, Kannan, and Sarosh Irani. Investigation in neurological disease. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0221.

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This chapter provides a brief overview of the more commonly available neurophysiology and neuroradiology techniques, guidance on how to perform a lumbar puncture, and a summary of biochemical, immunological, and genetic tests relevant to neurological disorders.
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Handbook of Immunological Investigations in Children. Elsevier, 1990. http://dx.doi.org/10.1016/c2013-0-06488-2.

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Book chapters on the topic "Immunological investigation"

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Birkett, Christopher R., and Keith Gull. "Immunological Approaches and Techniques Employed in Tubulin and Microtubule Research." In Investigation and Exploitation of Antibody Combining Sites, 153–66. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5006-4_19.

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Satoh, Hiroko, James R. Gillette, Lance R. Pohl, Tamiko Takemura, Victor J. Ferrans, Sandra E. Jelenich, John G. Kenna, and James Neuberger. "Investigation of the Immunological Basis of Halothane-Induced Hepatotoxicity." In Biological Reactive Intermediates III, 657–73. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-5134-4_61.

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Raff, Martin C. "An Immunological Approach to the Cell Lineage Problem in the Mammalian CNS." In Investigation and Exploitation of Antibody Combining Sites, 217–18. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5006-4_24.

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Bernauer, W., and P. H. Itin. "Immunological Investigations." In Cicatrising Conjunctivitis, 102–10. Basel: KARGER, 1997. http://dx.doi.org/10.1159/000060708.

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Plioplys, Audrius V. "Autism: Immunological Investigations." In Diagnosis and Treatment of Autism, 133–38. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4899-0882-7_9.

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Anderson, Graham, and Eric J. Jenkinson. "Investigating Central Tolerance With Reaggregate Thymus Organ Cultures." In Immunological Tolerance, 185–96. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-395-0_11.

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Savorani, Giancarlo, Gabriele Sarti, Afro Salsi, Francesco Cavazzuti, Lucio Zanichelli, Giuseppe Tucci, Eugenio Mocchegiani, Nicola Fabris, Mariella Chiricolo, and Federico Licastro. "Immunological Investigation of Thymic-Dependent Immunity in Normal Aging and in Patients with Senile Dementia of Alzheimer Type." In Biomedical Advances in Aging, 125–33. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0513-2_10.

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Butt, W. R., A. C. Crooke, and F. J. Cunningham. "Immunological Investigations of Human Gonadotropins." In Ciba Foundation Symposium - Immunoassay of Hormones (Colloquia on Endocrinology, Vol. 14), 310–28. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470719299.ch17.

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Bauer, Rudolf. "Chemistry, analysis and immunological investigations of Echinacea phytopharmaceuticals." In Immunomodulatory Agents from Plants, 41–88. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8763-2_2.

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Coscia, Maria Rosaria, and Umberto Oreste. "Immunological Investigations on Antarctic fish Parasitism by Nematodes." In New Developments in Marine Biotechnology, 213–16. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4757-5983-9_45.

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Conference papers on the topic "Immunological investigation"

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Kozlovskaya, G. V., M. I. Zinevich, Y. E. Kozlovsky, T. I. Khomyakova, and A. D. Magomedova. "IMMUNOLOGICAL ASPECT OF ORAL ADMINISTRATION OF THE PROBIOTIC LACTOBACILLUS CASEI LB 148 UNDER COLD STRESS IN ANIMALS." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS Volume 2. DSTU-Print, 2020. http://dx.doi.org/10.23947/interagro.2020.349-351.

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Gut microbiome changes is considered as a basic causative factor of stress-associated diseases. Probiotics are usually used for the correction of disbiosis. The aim of the investigation was the study of the effects of oral probiotic Lactobacillus casei LB 148 use onto the gut microbiota as well as the number and total square of intestinal lymph nodules of rats Sprague Dawley at in health rats and under the cold stress
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Koller, U., I. Pabinger, K. Lechner, and W. Knapp. "HEAT INACTIVATED HIGHLY PURIFIED FACTOR VIII CONCENTRATE IN THE TREATMENT OF HEMOPHILIACS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644057.

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51 severe hemophiliacs who had previously been treated with not virus-inactivated intermediate purity factor VIII concentrates were divided into two groups according to their immunological status. Group A (n=23) consisted of patients with CD4/CD8 (helper/suppressor) T cell ratio of 1.0, group B (n=28) of . patients with a ratio of 1.0. In patients of group A treatment was switched in May 1983 to highly purified heat inactivated factor VIII concentrate (BEHRINGWERKE GmbH, Marburg) whereas patients of group B continued to receive intermediate purity factor VIII concentrate. In both groups laboratory tests (clinical investigation, routine liver function tests, differential blood count, lymphocyte subpopulations and quantitative immunoglobulin analysis) were performed in May 1983 and repeated 6, 12 and 18 months thereafter. In group A a significant reduction (p 0.005) of CD8 positive cells from 10587/μl (median) to 540/μl (18 months) was observed; no significant changes of CD8 positive cells occurred in group B. CD4/CD8 ratio rose from 0.58 to 0.86 in group A (p = 0.005) and remained unchanged in group B (1.38 versus 1.23). Serum IgG decreased in both groups but was more pronounced in group A. Thus treatment with heat inactivated highly purified factor VIII improved the immunological status of hemophiliacs with an inverse ratio. Retrospective analysis of antibodies to HIV, however, showed that most of the patients of group A were antibody positive (n=21), but the 2 negative patients remained negative. In group B of the 10 HIV negative patients one became positive, all others did not change. Whether this improvement of immunological laboratory findings is of clinical relevance, remains to be established, particularly with respect to the high incidence of antibody positive patients within group A.
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Anarkulov, Talant Nematovich. "The investigation of immunological reactivity and the state of actual nutrition of qualified kureshists (fighters) during an annual cycle of sport training." In VIII International Research-to-practice conference. TSNS Interaktiv Plus, 2017. http://dx.doi.org/10.21661/r-117094.

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Fang, Hsu-Wei, and Yu-Chih Su. "Effects of Biological Lubrication on the Morphology of UHMWPE Wear Particles Generated With Surface Textures." In World Tribology Congress III. ASMEDC, 2005. http://dx.doi.org/10.1115/wtc2005-63501.

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Osteolysis induced by ultra-high molecular weight polyethylene (UHMWPE) wear particles has emerged as a critical failure mechanism of artificial joints. Many researchers have performed cell culture studies or animal tests to investigate the UHMWPE particles induced immunological responses. However, the effect of size and shape remains elusive. This is partly because of the difficulty in obtaining UHMWPE wear particles with specific sizes and shapes for bioactivity tests. Previously, we have shown the feasibility of producing narrowly distributed UHMWPE particle sizes and shapes by rubbing UHMWPE pins against textured surfaces [1]. The correlations between the surface texture dimensions and the size and shape of wear particles generated in water have been obtained [2]. In addition, this novel technique of UHMWPE particle generation enables the investigation of the effects of UHMWPE particle size and shape on bioactivity. It is concluded that the elongated particles and the particles which can be engulfed by macrophage cells induced stronger immunological responses [3]. Thus, it is possible to enhance the life of total joint replacements by reducing the production of the most toxic particle populations in terms of size and shape. Our idea is to apply surface textures on the articulating surface of joint implant in order to control the size and shape of UHMWPE wear particles. While maintaining a low wear rate of UHMWPE parts, further reduction of the most “toxic” particles released into human body shall prevent particle-induced osteolysis.
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Krasnoshtanova, Alla, and Alesya Yudina. "PRODUCTION OF ANTIBODIES FROM POULTRY YOLK (IgY) AND INVESTIGATION OF THEIR IMMUNOCHEMICAL PROPERTIES." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/17.

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"A particularly important aspect of immunology is to develop non-invasive methods of obtaining antibodies which could be a great alternative to traditional ones that based on the harmful procedure of isolation of immunoglobulins from animal blood sera. That’s why the extraction of antibodies from poultry egg yolks (IgY) is the most promising. Due to the fact of variation of IgY structural features that determine the definite immunochemical properties, yolk antibodies in comparison with mammalian immunoglobulins (IgG) does not interact with rheumatoid factor (Rf), contribute to the activation of the complement system, bind to the Fc-receptor (FcR), and also has weak cross-reactivity, which confirms the possibility of their widespread use in medicine and food. Also the presence of phylogenetic distance between chickens and mammalians guarantees immune response against conservative mammalian protein molecules which is highly important for the creation of new generation test systems. The aim of this work is to develop a selective method of producing high-purity immunoglobulin Y preparations from the yolk of chicken eggs. There were adopted selective conditions of isolation of IgY under spontaneous thawing procedure at the room temperature of firstly frozen yolk solution in a sodium-phosphate buffer mixed with water (pH 5.0) in a ratio of 1:6, which leads to receiving a water-soluble fraction further precipitated with the sodium chloride at a concentration of 10% of the solution mass and subsequently concentrated using ultrafiltration with membrane UAM-10, that allows achieving the content of IgY not less than 95% per dry substance in immunoglobulin fraction. It is possible to produce a protein fraction with a protein content of at least 9 g/l. The purity of the immunoglobulin fraction was verified using polyacrylamide gel electrophoresis. The presence of a light chain in the IgY solution was proved to be a low-molecular compound using the method of gel-filtration-chromatography. The immunological activity of IgY was studied with respect to bovine serum albumin (BSA) as an antigen. The enzymatic resistance of IgY against proteolytic enzymes was tested in area of the gastrointestinal tract."
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Shchyogolev, Sergei Y., Nikolai G. Khlebtsov, and Boris I. Schwartsburd. "Spectroturbidimitry as applied to biomedical and immunological investigations." In Radiofrequency and Optical Methods of Biomedical Diagnostics and Therapy, edited by Valery V. Tuchin. SPIE, 1993. http://dx.doi.org/10.1117/12.146490.

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Fernandes, D., J. Cannon, B. Fitzgerald, J. Welch, M. Jones, P. Cullinan, and J. Szram. "S104 Investigating the diagnostic performance of specific immunological tests in occupational asthma." In British Thoracic Society Winter Meeting 2017, QEII Centre Broad Sanctuary Westminster London SW1P 3EE, 6 to 8 December 2017, Programme and Abstracts. BMJ Publishing Group Ltd and British Thoracic Society, 2017. http://dx.doi.org/10.1136/thoraxjnl-2017-210983.110.

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Robinson, George, Kirsty Waddington, Leda Coelewij, Junjie Peng, Anna Radziszewska, Chris Wincup, Hannah Peckham, et al. "P171 Cardiovascular risk in young patients with lupus: investigating metabolic biomarkers and the immunological phenotype." In 12th European Lupus Meeting. Lupus Foundation of America, 2020. http://dx.doi.org/10.1136/lupus-2020-eurolupus.213.

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Otsuki, Takemi, Suni Lee, Hidenori Matsuzaki, Naoko Kumagai-Takei, Kei Yoshitome, and Yasumitsu Nishimura. "416 Search for biomarkers of asbestos exposure and asbestos-induced cancers in investigations of the immunological effects of asbestos." In 32nd Triennial Congress of the International Commission on Occupational Health (ICOH), Dublin, Ireland, 29th April to 4th May 2018. BMJ Publishing Group Ltd, 2018. http://dx.doi.org/10.1136/oemed-2018-icohabstracts.159.

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Andrew, M., B. A. Paes, R. A. Milner, P. J. Powers, M. Johnston, and V. Castle. "THE POSTNATAL DEVELOPMENT OF THE COAGULATION SYSTEM IN THE PREMATURE INFANT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643606.

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A cohort study was performed to determine the postnatal development of the coagulation system in the “healthy” premature infant. Mothers were approached for consent and a total of 132 premature infants were entered into the study. The group consisted of 64 infants with gestational ages of 34-36 weeks (prem 1) and 68 infants whose gestational age was 33 weeks or less (prem 2). Demographic information and a 2 ml blood sample were obtained on days 1, 5, 30, 90, and 180. Plasma was fractionated and stored at −70°C for batch assaying of the following tests: screening tests, PT, APTT; factor assays (biologic (B)); fibrinogen, II, V, VII, VIII:C, IX, X, XI, XII, prekallikrein, high molecular weight kininogen, XIII (immunologic (I)); inhibitors (I), antithrombin III, aα2-antiplasmin, α2-macroglobulin, α-anti-trypsin, Cl esterase inhibitor, protein C, protein S, and the fibrinolytic system (B); plasminogen. We have previously reported an identical study for 118 full term infants. The large number of premature and full term infants studied at varying time points allowed us to determine the following: 1) coagulation tests vary with the gestational age and postnatal age of the infant; 2) each factor has a unique postnatal pattern of maturation; 3) near adult values are achieved by 6 months of age; 4) premature infants have a more rapid postnatal development of the coagulation system compared to the full term infant; and 5) the range of reference values for two age groups of premature infants has been established for each of the assays. These reference values will provide a basis for future investigation of specific hemorrhagic and thrombotic problems in the newborn infant.
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