Academic literature on the topic 'Imp3p'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Imp3p.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Imp3p"
1
Lee, Sarah J., and Susan J. Baserga. "Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing." Molecular and Cellular Biology 19, no. 8 (August 1, 1999): 5441–52. http://dx.doi.org/10.1128/mcb.19.8.5441.
Full textAbstract:
ABSTRACT The function of the U3 small nucleolar ribonucleoprotein (snoRNP) is central to the events surrounding pre-rRNA processing, as evidenced by the severe defects in cleavage of pre-18S rRNA precursors observed upon depletion of the U3 RNA and its unique protein components. Although the precise function of each component remains unclear, since U3 snoRNA levels remain unchanged upon genetic depletion of these proteins, it is likely that the proteins themselves have significant roles in the cleavage reactions. Here we report the identification of two previously undescribed protein components of the U3 snoRNP, representing the first snoRNP components identified by using the two-hybrid methodology. By screening for proteins that physically associate with the U3 snoRNP-specific protein, Mpp10p, we have identified Imp3p (22 kDa) and Imp4p (34 kDa) (named for interacting with Mpp10p). The genes encoding both proteins are essential in yeast. Genetic depletion reveals that both proteins are critical for U3 snoRNP function in pre-18S rRNA processing at the A0, A1, and A2 sites in the pre-rRNA. Both Imp proteins associate with Mpp10p in vivo, and both are complexed only with the U3 snoRNA. Conservation of RNA binding domains between Imp3p and the S4 family of ribosomal proteins suggests that it might associate with RNA directly. However, as with other U3 snoRNP-specific proteins, neither Imp3p nor Imp4p is required for maintenance of U3 snoRNA integrity. Imp3p and Imp4p are therefore novel protein components specific to the U3 snoRNP with critical roles in pre-rRNA cleavage events.
2
Wehner, Karen A., Jennifer E. G. Gallagher, and Susan J. Baserga. "Components of an Interdependent Unit within the SSU Processome Regulate and Mediate Its Activity." Molecular and Cellular Biology 22, no. 20 (October 15, 2002): 7258–67. http://dx.doi.org/10.1128/mcb.22.20.7258-7267.2002.
Full textAbstract:
ABSTRACT The SSU processome is required for production of the small ribosomal subunit RNA, the 18S rRNA. Specifically, the U3 small nucleolar RNA (snoRNA) component of the SSU processome is essential for the formation of the conserved central pseudoknot and for cleavages of the pre-rRNA, both of which are required for 18S maturation. To further elucidate how these events are mediated, we examined the regulatory and mechanistic roles of the U3 specific proteins: Imp3p, Imp4p, and Mpp10p. We found that these proteins demonstrated an interdependence with respect to their stability and to their association with the U3 snoRNA. Because mutations in the U3 snoRNA that disrupt pre-rRNA processing confer similar defects on growth and pre-rRNA processing as do carboxy-terminal truncations of Mpp10p, we hypothesized that Mpp10p may be involved in maintaining U3 snoRNA-pre-rRNA base pairing. However, combining the two mutations resulted in a more pronounced cleavage defect at site A2, suggesting that Mpp10p is also required at an additional mechanistic step. Furthermore, heterologous complementation experiments demonstrate that the last 95 amino acids of yeast Mpp10p are specifically required for growth and pre-rRNA processing at low temperatures.
3
Gerczei, T., and C. C. Correll. "Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA." Proceedings of the National Academy of Sciences 101, no. 43 (October 15, 2004): 15301–6. http://dx.doi.org/10.1073/pnas.0406819101.
Full text
4
Hsieh, Yi-Ching, Pei-Jung Tu, Ying-Yuan Lee, Chun-Chen Kuo, Yi-Chien Lin, Chi-Fang Wu, and Jing-Jer Lin. "The U3 small nucleolar ribonucleoprotein component Imp4p is a telomeric DNA-binding protein." Biochemical Journal 408, no. 3 (November 28, 2007): 387–93. http://dx.doi.org/10.1042/bj20070968.
Full textAbstract:
Imp4p is a component of U3 snoRNP (small nucleolar ribonucleoprotein) involved in the maturation of 18S rRNA. We have shown that Imp4p interacts with Cdc13p, a single-stranded telomere-binding protein involved in telomere maintenance. To understand the role of Imp4p in telomeres, we purified recombinant Imp4p protein and tested its binding activity towards telomeric DNA using electrophoretic mobility-shift assays. Our results showed that Imp4p bound specifically to single-stranded telomeric DNA in vitro. The interaction of Imp4p to telomeres in vivo was also demonstrated by chromatin immunoprecipitation experiments. Significantly, the binding of Imp4p to telomeres was not limited to yeast proteins, since the hImp4 (human Imp4) also bound to vertebrate single-stranded telomeric DNA. Thus we conclude that Imp4p is a novel telomeric DNA-binding protein that, in addition to its role in rRNA processing, might participate in telomere function.
5
Feng, Wei, Zhongren Zhou, Jeffrey H. Peters, Thaer Khoury, Qihui Zhai, Qiying Wei, Camtu D. Truong, Sonya Wei Song, and Dongfeng Tan. "Expression of Insulin-Like Growth Factor II mRNA-Binding Protein 3 in Human Esophageal Adenocarcinoma and Its Precursor Lesions." Archives of Pathology & Laboratory Medicine 135, no. 8 (August 1, 2011): 1024–31. http://dx.doi.org/10.5858/2009-0617-oar2.
Full textAbstract:
Context.—Insulin-like growth factor II mRNA-binding protein 3 (IMP3) is an oncofetal protein highly expressed in fetal tissue and malignant tumors but only rarely within adult benign tissues. The expression of IMP3 in esophageal adenocarcinoma (EAC) and its precursor lesions including distinctive type Barrett mucosa (BM, intestinal metaplasia) and esophageal columnar dysplasia (ECD) is largely unknown. Objective.—To characterize the patterns of IMP3 expression in EAC and its precursor lesions. Design.—Samples from 132 cases of EAC, 28 cases of ECD (16 high-grade dysplasia and 12 low-grade dysplasia cases), 28 cases of BM without dysplasia, and 138 cases of nonneoplastic esophageal mucosa without dysplasia or BM within formalin-fixed, paraffin-embedded tissue microarray blocks were examined. Tissues were stained with mouse monoclonal anti-IMP3 antibody. The intensity (1–3+) and percent (0%–100%) of positive cytoplasmic and/or membranous IMP3 staining cells were determined. Results.—Most of EAC cases (93 of 132; 70%) showed cytoplasmic and membranous IMP3 staining. Poorly and moderately differentiated EAC showed statistically significant higher IMP3 expression compared with well-differentiated EAC (P < .001). A subset of ECD cases (7 of 28; 25%) was positive for IMP3, including 3 low-grade dysplasia cases (focal 1+ IMP3 staining) and 4 high-grade dysplasia cases (more diffuse 1–2+ IMP3 staining). No IMP3 staining was observed in any nonneoplastic esophageal mucosa and BM tissues without dysplasia. Conclusions.—This study suggests that IMP3 may play a role in the carcinogenesis of EAC and has diagnostic utility in differentiating neoplastic and nonneoplastic lesions of the esophagus.
6
Hao, Suyang, Thomas W. Smith, Peigou G. Chu, Qin Liu, Chi Young Ok, Bruce A. Woda, Di Lu, et al. "The Oncofetal Protein IMP3: A Novel Molecular Marker to Predict Aggressive Meningioma." Archives of Pathology & Laboratory Medicine 135, no. 8 (August 1, 2011): 1032–36. http://dx.doi.org/10.5858/2009-0652-oar2.
Full textAbstract:
Context.—One of the major clinical challenges is to predict recurrence of meningioma. Recently, we have found that IMP3, an oncofetal RNA-binding protein, is a biomarker to predict aggressive tumors. Objective.—To investigate whether IMP3 can be used as a new biomarker to predict the recurrence and overall survival of patients with meningiomas. Design.—One hundred seven patients with primary meningiomas were investigated for expression of IMP3 by immunohistochemistry and whether expression of this molecule correlated with tumor recurrence and overall survival. Results.—Tumor recurrence was found in 13 of 107 patients with primary meningioma. Seven of 107 patients (6.5%) with primary meningiomas expressed IMP3. Kaplan-Meier plots and log-rank tests showed that patients with IMP3-positive tumors had a much higher recurrence rate (P = .004) and a poorer overall survival (P < .001) than did patients with IMP3-negative tumors. The 5-year recurrence-free and overall survival rates were 0% and 36% in IMP3-positive patients versus 89% and 94% in IMP3-negative patients, respectively. Multivariable analysis of IMP3 status (positive versus negative) in primary tumors showed a hazard ratio of 17.89 for recurrence (P = .01), which was much higher than hazard ratios associated with all other known risk factors including higher tumor grades and Ki-67 labeling index. Conclusions.—IMP3 is a potential independent prognostic biomarker that can be used at the time of initial diagnosis of meningioma to identify patients who have a high risk of developing a recurrence.
7
Plum, Patrick, Dita Ulase, Seung-Hun Chon, Felix Berlth, Thomas Zander, Hakan Alakus, Elfriede Bollschweiler, et al. "PS02.197: UPREGULATION OF IMP3 HAS A NEGATIVE PROGNOSTIC IMPACT IN EARLY INVASIVE ESOPHAGEAL ADENOCARCINOMA." Diseases of the Esophagus 31, Supplement_1 (September 1, 2018): 178. http://dx.doi.org/10.1093/dote/doy089.ps02.197.
Full textAbstract:
Abstract Background Despite improvements in perioperative treatments, the overall survival of patients with esophageal adenocarcinoma (EAC) remains low. In early invasive tumors (pT1) there is striking risk discrepancy for tumors with vascular invasion (L1/V1). As this tumor stage is potentially locally resectable, there is an urgent need for reliable pretherapeutical evaluation. High expression of IMP3 correlates with worse prognosis in colon and gastric cancer. In a small series of 30 patients with EAC, IMP3 is significantly associated with lymphovascular invasion. In our large cohort of EAC, we analyzed the immunohistochemical expression of IMP3 in correlation to clinical data. Methods 371 patients who underwent esophagectomy due to EAC at the Department of General, Visceral and Cancer Surgery, University of Cologne between 1999 and 2012 were included. Tissue Microarrays (TMA) were retrospectively established from the formalin-fixed, paraffin embedded material of the resected specimens and immunohistochemically stained using the primary antibody specific for IMP3. The IMP3 staining intensity was scored manually according to a 3-tier-scoring system (negative, weak and strong). Results TMAs from 371 patients were interpretable for further analysis. 109 patients (29.3%) had superficial EAC (pT1a/pT1b) while 262 (70.7%) showed locally advanced tumors (pT2 or pT3). 67 (26.7%) patients with advanced tumors and all pT1 EAC patients did not receive neoadjuvant therapy (chemotherapy or chemo-radiotherapy) prior to surgery. 259 of the 371 EAC revealed positive immunostaining for IMP3 including 167 strongly and 92 weakly positive. For patients without neoadjuvant therapy, there was a significant trend for higher IMP3 expression in early invasive tumors (pT1a/pT1b) showing significantly higher rates of IMP3 positive tumors compared to locally advanced pT3-tumors without neoadjuvant therapy (P = 0.0015). There was no difference of IMP3 expression according to neoadjuvant therapy in patients with pretreated EAC. There is a statistically significant association between IMP3 protein expression and shortened survival in early invasive pT1-esophageal carcinomas (P = 0.045). Conclusion IMP3 is a feasible marker in early invasive EAC (pT1a and pT1b) and is significantly associated with worse outcome. IMP3 indicates lymph node metastases, advanced infiltration depth and higher tumor grade. Thus IMP3 might be useful for therapeutic decisions in early invasive EAC. Disclosure All authors have declared no conflicts of interest.
8
Wei, Ping, Dawei Li, Ye Xu, Sanjun Cai, and Xiang Du. "Effect of IMP3 on epithelial-to-mesenchymal transition and the function of colon cancer stem cells." Journal of Clinical Oncology 33, no. 3_suppl (January 20, 2015): 631. http://dx.doi.org/10.1200/jco.2015.33.3_suppl.631.
Full textAbstract:
631 Background: EMT could promote the acquisition of stem-like properties in cancer cells and cancer stem cell (CSC) has EMT properties. However, the underlying mechanism of the interaction between EMT and CSC still remain unclear. We previously identified Insulin-like growth factor-II mRNA-binding protein 3 (IMP3) plays a vital role in colon cancer carcinogenesis. Here, we aimed to identify the gene function between EMT and CSC in colon cancer. Methods: The expression of IMP3 was analyzed in human tumor tissues. Colonospheres were isolated from colon cancer cell lines and the biological features of IMP3 were investigated in the process of EMT and colonoshpere cells. Results: Increased IMP3 levels were significantly correlated with higher clinical stage, T classification, LNM, presence of distant metastasis. Patients with IMP3-positive localized tumors had lower 5-year disease-free survival (DFS) and overall survival (OS) than those with IMP3-negative tumors. Multivariate survival analysis showed that IMP3 was an independent prognostic marker for DFS and OS. Expanded colonospheres contained cells that expressed high levels of CD133 and had the ability to promote migration and activate the epithelial-mesenchymal transition. Down regulation of IMP3 in HCT116 cells inhibited the invasion, migration ability and epithelial-mesenchymal transition and the properties of colonospheres. Conclusions: These findings suggest that IMP3 plays an important role in colon cancer tumorigenesis and metastasis through transactivation of colon CSC with EMT property, which is helpful to identify critical marker and therapeutic target.
9
Del Gobbo, Alessandro, Emanuela Bonoldi, Fulvia Milena Cribiù, Ilaria Franceschetti, Caterina Matinato, Stefano Fiori, Umberto Gianelli, and Silvano Bosari. "Insulin-like growth factor II mRNA binding protein 3 (IMP3) expression in cervical intraepithelial neoplasia and its relationship with HIV-infection status." Sexual Health 12, no. 1 (2015): 22. http://dx.doi.org/10.1071/sh13144.
Full textAbstract:
Background Cervical cancer is preventable through screening, and HIV treatment guidelines recommend that all HIV-infected women receive cervical cancer twice during the year after HIV diagnosis and annually thereafter. Different immunohistochemical markers have been studied to highlight cervical intraepithelial lesions of low and high grade, the most widely used being p16. Recent studies have shown that insulin-like growth factor mRNA binding protein 3 (IMP3) plays a role in the development of invasive squamous cell carcinoma from cervical dysplasia, both in histology and in liquid-based cytology. Methods: We evaluated the clinical significance of the immunohistochemical expression of IMP3 and p16 in histological samples of cervical intraepithelial neoplasia from 56 samples of HIV-positive and 30 samples of HIV-negative patients. Results: A significant difference was found in IMP3 and p16 protein expression between HIV-positive and HIV-negative specimens. All cases of HIV-positive low grade squamous intraepithelial neoplasia (L-SIL) with IMP3 expression progressed in high grade (H)-SIL. However, the HIV-positive patients with IMP3-negative L-SIL remained stable or had a negative follow up. The L-SIL of HIV-negative patients with IMP3 protein expression had an uneventful follow up. IMP3-positive H-SIL recurred with low- or high-grade dysplasia during follow up after conisation in both populations. All IMP3-negative L-SIL and H-SIL had negative pap tests at follow up. Conclusions: In HIV-positive cases, IMP3 showed a higher sensitivity than p16 in identifying patients at risk of progression and recurrence.
10
Bucelli, Robert C., and Alan Pestronk. "Immune myopathies with perimysial pathology." Neurology - Neuroimmunology Neuroinflammation 5, no. 2 (January 18, 2018): e434. http://dx.doi.org/10.1212/nxi.0000000000000434.
Full textAbstract:
ObjectiveImmune myopathies with perimysial pathology (IMPP) have a combination of damage to perimysial connective tissue and muscle fiber necrosis, more prominent near the perimysium. We studied the clinical and laboratory correlates of patients with pathologically defined IMPP.MethodsThis is a retrospective chart and pathology review of 57 consecutive patients with IMPP myopathology and, for comparison, 20 patients with dermatomyositis with vascular pathology (DM-VP).ResultsCompared with DM-VP, IMPP patients more commonly had interstitial lung disease (ILD) (p < 0.01), Raynaud phenomenon (p < 0.05), mechanic's hands (p < 0.05), arthralgias (p < 0.001), and a sustained response to immunomodulatory therapy (p < 0.05), and less frequently had a concurrent malignancy (p < 0.01). IMPP patients had higher serum creatine kinase values (p < 0.05), more frequent serum Jo-1 (p < 0.03) or SSA/SSA52 autoantibodies (p < 0.05), and less frequent antinuclear antibodies (p < 0.01). IMPP patients with serum Jo-1/antisynthetase antibodies were more likely to have ILD (p < 0.05) and inflammatory arthritis (p < 0.05) than IMPP patients without these antibodies.ConclusionsIMPP myopathology is associated with an increased risk of ILD, Raynaud phenomenon, mechanic's hands, and inflammatory arthritis when compared with another immune myopathy (DM-VP). IMPP patients require regular screening for ILD, particularly those with antisynthetase antibodies. The absence of myositis-specific autoantibodies in a large percentage of IMPP patients emphasizes the important role for myopathology in identifying patients at higher risk of severe comorbid conditions such as ILD.
Dissertations / Theses on the topic "Imp3p"
1
Cosnier, Bruno. "Etude fonctionnelle des protéines Sup35 et Imp3 chez la levure Saccharomyces cerevisiae." Paris 11, 2008. http://www.theses.fr/2008PA112307.
Full textAbstract:
Le domaine C-terminal très conservé de la protéine Sup35, impliquée dans la terminaison de la traduction, possède un site potentiel de phosphorylation par la PKA au niveau de la Thréonine341. Nous avons recherché si ce résidu était phosphorylé in vivo et s’il était impliqué dans la régulation fonctionnelle de la protéine. Dans les conditions testées, aucune phosphorylation de Sup35p n’a pu être mise en évidence in vivo mais nous avons montré que le résidu T341 était critique pour la fonctionnalité de la protéine et pourrait être impliqué dans des interactions fonctionnelles entre les domaines N et C terminaux. Le domaine N de Sup35p est responsable du phénotype prion [PSI+]. Jusqu’à présent, les seules mutations caractérisées influençant les propriétés prion de cette protéine ont été localisées dans ce domaine N-terminal. Nous avons identifié une mutation dans le domaine C-terminal qui modifie les capacités d’agrégation de la protéine. Cette observation apporte de nouveaux éléments pour la compréhension du mécanisme de conversion de Sup35p vers un état agrégé. IMP3 est un gène essentiel codant une protéine impliquée dans la biogenèse des ribosomes. Nous avons construit une souche capable d’exprimer de façon endogène un allèle mutant hypomorphe du gène IMP3. Cette souche présente d’importants défauts de biogenèse de la petite sous unité 40S du ribosome. Nous avons montré que la fidélité de la traduction est affectée dans cette souche : l’efficacité de décalage du cadre lecture en +1 est augmentée. Nos expériences montrent que cette protéine pourrait être également impliquée dans la réparation de l’ADN et le contrôle de la taille des télomèresAll Sup35 homologs share a potential phosphorylation site at threonine 341, suggesting a functional role for this residue. We investigated whether this residue is actually phosphorylated in yeast and if it is involved in the termination activity of the protein. In the conditions we tested, no phosphorylation of the Sup35 protein in vivo was detected. However our results point to a new critical residue involved in the translation termination activity of Sup35p and in functional interaction between the N- and C-domains of the protein. The N-terminal domain of Sup35p is required for prion propagation, driving the switch from the soluble, functional [psi-] state to the insoluble [PSI+] prion state. To date, all the critical elements for prion induction and propagation have been mapped to the N domain of the protein. Here we report for the first time a mutation in the C-terminal domain of Sup35p which alters the aggregation properties of Sup35p. This observation has important consequence for understanding the mechanism of prion conversion. The essential IMP3 gene encodes a component of the SSU processome, a large ribonucleoprotein required for processing of small subunit rRNA precursors. We constructed and analysed a mutant of the IMP3 gene able to sustain cell growth. A strain expressing this hypomorphic allele displayed ribosome biogenesis defects characteristic of a depletion in Imp3p. We demonstrated the +1 frameshifting was increased in the mutant strain. Our further characterization revealed involvement of the Imp3 protein in DNA repair and telomere length control, two pathways that are not directly related to ribosome biogenesis
2
Oberski, Vanessa [Verfasser], and Andreas [Gutachter] Rosenwald. "IMP3-Expression in Mantelzelllymphomen / Vanessa Oberski ; Gutachter: Andreas Rosenwald." Würzburg : Universität Würzburg, 2018. http://d-nb.info/1169573002/34.
Full text
3
Deforzh, Evgeny. "Le complexe IMP3 protège ses ARNm cibles de la répression traductionnelle dépendante de Argonaute/GW182/miRNA." Thesis, Université Paris-Saclay (ComUE), 2015. http://www.theses.fr/2015SACLS203/document.
Full textAbstract:
Les protéines se liant à l’ARN de la famille IMP sont les protéines oncofoetales conservées, qui régulent le transport, la stabilité et la traduction de plusieurs ARNm cibles. Les IMPs sont impliqués dans la tumorigenèse et dans le développement embryonnaire par le contrôle de la prolifération cellulaire, la différenciation, la migration, la polarisation et d`autres processus cellulaires. IMP-3 est difficilement détectable dans des tissus adultes normaux, mais il est surexprimé dans les nombreux cancers, où il a été caractérisé comme un marqueur d’agressivité et de la croissance tumorale rapide, ainsi que d’un pronostic défavorable pour les patients. Dans notre étude, nous avons utilisé une lignée cellulaire RD de rhabdomyosarcome (RMS), où IMPs étaient initialement décrits comme des protéines régulatrices de l`ARNm de IGF-2. Nous avons essayé d'élucider le mécanisme par lequel IMP3 régule l’expression des cyclines D1 et D3, contribuant ainsi à la compréhension des processus oncogéniques dans les RMS et autres cancers.Nous avons montré que IMP3 régule l'expression des cyclines D1 et D3 d'une manière significative in vivo. Nous avons également démontré, qu'en absence de IMP3, les ARNm des cyclines sont exportés vers le cytoplasme et s’associent avec les polyribosomes, mais ne sont pas traduits. En outre, l'inhibition d`IMP3 n'a pas d'influence sur la stabilité des ARNm des cyclines. Nous démontrons que dans des cellules cancéreuses humaines, IMP3 interagit avec plusieurs protéines se liant à l'ARN, et que nombre de ces protéines a un effet sul l’expression des cyclines, ce que suggère l'existence d'un complexe régulateur multiprotéique sur les 3'UTR des cyclines D1 et D3. Nos résultat montrent que l'inhibition de deux protéines clés de RNA-induced silencing complex (RISC) (AGO2 et GW182/TNRC6), rétablit les niveaux d'expression des cyclines D1 et D3, qui ont été considérablement diminués en l’absence d’IMP3 ou de ses partenaires protéiques ILF3/NF90 et PTBP1. Nous concluons que les complexes d`IMP3 et RISC peuvent concourir pour la régulation des ARNm des cyclines. Nous avons également identifié les miARNs qui peuvent être impliqués dans ce processus, ainsi que les domaines fonctionnellement importants dans les 3 'UTR des cyclines, où se passe la competition entre les complexes d’IMP-3 et RISC. Nos résultats sont compatibles avec l'existence de IMP3 - contenant complexe multiprotéique, qui est associé à 3'UTRs des cyclines et régule leur traduction en les protégeant contre la répression traductionnelle par miRISCRNA-binding proteins of the IMP family (IGF2 mRNA-binding proteins 1-3) are conserved oncofetal proteins, regulating transport, stability and decay of multiple mRNAs. IMPs are involved in embryonic developement and tumorigenesis by controlling cell proliferation, differentation, migration, polarization and many other important aspects of cell function. IMP-3 is hardly detectable in normal adult tissues, but is overexpressed in many cancers, where it has been reported as a marker of tumor aggressiveness, rapid growth, and bad prognosis for patients. In our research we utilized a rhabdomyosarcoma (RMS) cell line RD, where IMPs were first described as IGF-2 mRNA regulating proteins. We aimed to elucidate the mechanism by which IMP3 regulates the expression of cyclins D1 and D3, thereby contributing to the understanding of oncogenic processes in RMS.In this study, we show that IMP3 regulates the expression of cyclin D1 and D3 in a significant manner in vivo. We also demonstrate that in the absence of IMP3, the mRNAs of the cyclins are exported to the cytoplasm and associated with polyribosomes, but not translated. IMP3 inhibition does not influence the stability of cyclin mRNAs. We demonstrate that in human cancer cells, IMP3 interacts with multiple RNA-binding proteins, and that a number of these IMP-3 partners impacts on the expression of cyclins D1 and D3. These observations suggest the existence of a regulatory IMP-3 containing RNP complex on the 3’UTR of mRNAs of cyclin D1 and D3. Our results show that an inhibition of two key proteins of RNA-induced silencing complex (RISC) (AGO2 and GW182/TNRC6) rescues the expression of cyclin D1 and D3 proteins, which is significantly decreased in the absence of IMP3 or its protein partners ILF3/NF90 and PTBP1. Therefore, IMP3 and RISC complexes can compete for cyclin mRNAs translational repression/activation. We also identified a number of miRNAs that can be involved in this process, and characterized functionally important regions within 3’ UTRs of the cyclins, where the competition between IMP-3 and RISC complexes takes place. Our results are consistent with the existence of IMP3 - containing multiprotein complex, which is associated with 3’UTRs of the cyclins and regulates their translation by protecting them from miRISC-dependent translational repression
4
Wang, Yiying, Lingmin Li, Yue Wang, Zeng Yuan, Wenjing Zhang, Kenneth Hatch, and Wenxin Zheng. "IMP3 as a cytoplasmic biomarker for early serous tubal carcinogenesis." BioMed Central, 2014. http://hdl.handle.net/10150/610179.
Full textAbstract:
BACKGROUND:Serous tubal intraepithelial carcinoma (STIC) and the p53 signature in tubal mucosa have been supported to be precursor lesions in high-grade serous carcinoma (HGSC) of the fallopian tube, ovary, and peritoneum. It remains critical to find biomarkers for precursor lesions in order to detect HGSCs efficiently. IMP3 is an oncoprotein that has been explored in human malignancies. No studies have specifically addressed the expression of IMP3 in precursor or early lesions of HGSC. The main purposes of this study are to evaluate if IMP3 plays any role in the process of pelvic serous carcinogenesis by examining its expression in HGSC precursor lesions, to examine the relationship between IMP3 and p53 in those precursor lesions, and to check if IMP3 can be used as a biomarker for early diagnosis.METHODS:Immunohistochemistry for IMP3 and p53 was performed and evaluated in 48 HGSCs with STIC, 62 HGSCs without STIC, and 60 benign cases as negative controls. Sections of fallopian tubes with or without STIC , as well as cancers within the ovaries, were studied. IMP3 signature was defined as strong IMP3 cytoplasmic staining in 10 or more consecutive benign-looking tubal epithelial cells. The relationship between IMP3 and p53 overexpression was examined.RESULTS:In the 48 HGSC patients with STIC, IMP3 was positive in 46% of STIC lesions and had a similar positive rate in the invasive components of HGSC. IMP3 was also expressed in normal appearing tubal epithelia (IMP3 signature) in 15 (31%) of 48 HGSC cases with STIC and 10 (16%) of 62 cases without STIC. In contrast, no single IMP3 signature was found in the benign control group. Concordant expression of IMP3 and p53 signatures in the STIC group was found in up to one-third of the cases. There were also five (10%) STIC cases with positive IMP3 and negative p53.CONCLUSIONS:We conclude that IMP3 may be involved in the process and progression of pelvic HGSC and may serve as a complimentary biomarker in diagnosing STIC.
5
Ferreira, Brena Luize Cunha [UNESP]. "Determinação da relação de novo biomarcador (IMP3) no carcinoma de nasofaringe." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/131953.
Full textAbstract:
Made available in DSpace on 2015-12-10T14:22:44Z (GMT). No. of bitstreams: 0
Previous issue date: 2015-08-28. Added 1 bitstream(s) on 2015-12-10T14:28:56Z : No. of bitstreams: 1
000853894.pdf: 932709 bytes, checksum: 0fe73496b79c388c307c277adca72273 (MD5)Introdução:O Carcinoma de Nasofaringe(CNF) é uma neoplasia epitelial maligna agressiva, com principal incidência entre homens de 40-60 anos de idade, com distribuição geográfica e étnica variada. Está associado à infecção pelo Epstein- BarrVirus (EBV), e a presença deste nas células tumorais parece ter impacto no prognóstico. Porém, outros fatores que podem estar relacionados ao desfecho do CNF, têm sido pesquisados. O biomarcador IMP3, proteína de ligação ao mRNA que tem reconhecida função na ação das células tumorais, vem sendo analisado como um marcador factível correlacionado com a sobrevida global nos pacientes com câncer. Métodos: Para explorar a expressão do IMP3 nas amostras tumorais de pacientes com CNF vistos no Hospital das Clinicas da Faculdade de Medicina de Botucatu- UNESP, foram compilados 35 prontuários e documentados de acordo com as características morfológicas e tempo de sobrevida. A expressão imunohistoquímica foi mensurada e correlacionada com os diversos parâmetros clínicopatológicos. Conclusão: mostrou-se elevado índice de recidiva e morbimortalidade, com grande número de casos avançados ao diagnóstico, e potencial correlação de melhor prognóstico com a alta expressão do marcador analisado. Impacto: Um melhor entendimento do IMP3 comobiomarcador, proporcionando novas perspectivas no prognóstico do carcinoma de nasofaringe
Background: The nasopharyngeal carcinoma (NPC) is an agressive malignant epithelial neoplasm, mainly prevalent among 40-60-year-old men, with varied geographic and ethnic distribution. It is associated with Epstein Barr Virus (EBV), whose presence in the tumor cell seems to impact the prognosis. However, other factors that may be related to NPC's clinical impact and have been studied. The IMP3 biomarker, a mRNA binding protein, which has a recognized role in the tumor cell formation, has been analized as a feasible marker correlated to the overall survival of cancerpatients. Given this evidence, the object of this research isanalyze possible correlation of this biomarker to the clinical out come of these patients. Methods: To explore the IMP3 expression in the CNF patients' tumor samples seen at the Hospital das Clínicas da Faculdade de Medicina in Botucatu- UNESP, were compiled 35 cases and documented in basis of their morphological features and status of life. The immunohistochemical expression was measured and correlated with the several clinic-pathologic parameters. Results: The analysis showed high rates of relapse and mortality, also a significant number of local advanced tumors at diagnosis, and potential correlation of better prognosis with the high expression of the biomarkeranalized. Impact: A betterunderstandingof IMP3 as a biomarker, providing new routesofprognosis in nasopharynx carcinoma
6
Ferreira, Brena Luize Cunha. "Determinação da relação de novo biomarcador (IMP3) no carcinoma de nasofaringe /." Botucatu, 2015. http://hdl.handle.net/11449/131953.
Full textAbstract:
Orientador: Maria Aparecida Custódio DominguesCoorientador: Vitor Nakajima
Banca: Maria Luiza Cotrim Sartor de Oliveira
Banca: Alexandre Todorovic Fabro
Banca: Abina Altemani
Resumo: Introdução:O Carcinoma de Nasofaringe(CNF) é uma neoplasia epitelial maligna agressiva, com principal incidência entre homens de 40-60 anos de idade, com distribuição geográfica e étnica variada. Está associado à infecção pelo Epstein- BarrVirus (EBV), e a presença deste nas células tumorais parece ter impacto no prognóstico. Porém, outros fatores que podem estar relacionados ao desfecho do CNF, têm sido pesquisados. O biomarcador IMP3, proteína de ligação ao mRNA que tem reconhecida função na ação das células tumorais, vem sendo analisado como um marcador factível correlacionado com a sobrevida global nos pacientes com câncer. Métodos: Para explorar a expressão do IMP3 nas amostras tumorais de pacientes com CNF vistos no Hospital das Clinicas da Faculdade de Medicina de Botucatu- UNESP, foram compilados 35 prontuários e documentados de acordo com as características morfológicas e tempo de sobrevida. A expressão imunohistoquímica foi mensurada e correlacionada com os diversos parâmetros clínicopatológicos. Conclusão: mostrou-se elevado índice de recidiva e morbimortalidade, com grande número de casos avançados ao diagnóstico, e potencial correlação de melhor prognóstico com a alta expressão do marcador analisado. Impacto: Um melhor entendimento do IMP3 comobiomarcador, proporcionando novas perspectivas no prognóstico do carcinoma de nasofaringe
Abstract: Background: The nasopharyngeal carcinoma (NPC) is an agressive malignant epithelial neoplasm, mainly prevalent among 40-60-year-old men, with varied geographic and ethnic distribution. It is associated with Epstein Barr Virus (EBV), whose presence in the tumor cell seems to impact the prognosis. However, other factors that may be related to NPC's clinical impact and have been studied. The IMP3 biomarker, a mRNA binding protein, which has a recognized role in the tumor cell formation, has been analized as a feasible marker correlated to the overall survival of cancerpatients. Given this evidence, the object of this research isanalyze possible correlation of this biomarker to the clinical out come of these patients. Methods: To explore the IMP3 expression in the CNF patients' tumor samples seen at the Hospital das Clínicas da Faculdade de Medicina in Botucatu- UNESP, were compiled 35 cases and documented in basis of their morphological features and status of life. The immunohistochemical expression was measured and correlated with the several clinic-pathologic parameters. Results: The analysis showed high rates of relapse and mortality, also a significant number of local advanced tumors at diagnosis, and potential correlation of better prognosis with the high expression of the biomarkeranalized. Impact: A betterunderstandingof IMP3 as a biomarker, providing new routesofprognosis in nasopharynx carcinoma
Mestre
7
Wang, Yiying, Yue Wang, Dake Li, Lingmin Li, Wenjing Zhang, Guang Yao, Zhong Jiang, and Wenxin Zheng. "IMP3 signatures of fallopian tube: a risk for pelvic serous cancers." BioMed Central, 2014. http://hdl.handle.net/10150/610186.
Full textAbstract:
BACKGROUND:Recent advances suggest fallopian tube as the main cellular source for women's pelvic serous carcinoma (PSC). In addition to TP53 mutations, many other genetic changes are involved in pelvic serous carcinogenesis. IMP3 is an oncofetal protein which has recently been observed to be overexpressed in benign-looking tubal epithelia. Such findings prompted us to examine the relationship between IMP3 over-expression, patient age and the likelihood of development of PSC.METHODS:Fallopian tubes from three groups (low-risk, high-risk, and PSC) of patients with matched ages were studied. Age was recorded in 10years intervals ranging from age 20 to older than 80. The number of IMP3 signatures (defined by 10 or more tubal secretory cells stained positively and continuously in benign appearing tubal mucosa) from both tubal fimbria and ampulla segments was measured. The data was analyzed by standard contingency table and Poisson distribution methods after age adjustment. IMP3 overexpression was also examined in serous tubal intraepithelial carcinoma and PSC.RESULTS:The positive IMP3-stained cells are mainly tubal secretory cells. The absolute number of tubal IMP3 signatures increased significantly within each age group. Age remained a significant risk factor for serous neoplasia after age adjustment. IMP3 signatures were more frequent in the patients of both high-risk and PSC groups. The presence of IMP3 signatures in tubal mucosa was significantly associated with tubal or pelvic serous carcinogenesis (p<0.001).CONCLUSIONS:The findings suggest that tubal secretory cells with IMP3 signatures showing growth advantage could potentially serve as a latent precancer biomarker for tubal or pelvic serous carcinomas in women.
8
Moraes, Marcelo Padovani de Toledo [UNESP]. "Linfoma de Hodgkin: características anátomo-clínicas e análise de novo biomarcador (IMP3)." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/132038.
Full textAbstract:
Made available in DSpace on 2015-12-10T14:23:12Z (GMT). No. of bitstreams: 0
Previous issue date: 2015-02-19. Added 1 bitstream(s) on 2015-12-10T14:29:27Z : No. of bitstreams: 1
000849806.pdf: 849500 bytes, checksum: fbe135630fc95ef07e7c2a3ad5436dca (MD5)O Linfoma de Hodgkin (LH) é uma neoplasia hematopoiética que acomete caracteristicamente adultos jovens e apresenta, quando não tratado, curso clínico fatal. O diagnóstico precoce e preciso desse linfoma, associado à terapia moderna, por outro lado, tem proporcionado diminuição dramática das taxas de letalidade. Ainda assim, cerca de 15-20% dos pacientes evoluem de maneira insatisfatória com resistência a terapia primária ou recaída precoce pós-tratamento. Nesse sentido, há estudos variados na tentativa de buscar perfis prognósticos capazes de selecionar pacientes candidatos a terapias particulares. O IMP3 (insulin-like growth factor mRNA binding protein 3) tem sido descrito como biomarcador relacionado a pior prognóstico em diversas neoplasias, especialmente carcinomas, mas ainda pouco estudado em neoplasias hematopoiéticas. Para avaliar seu potencial papel prognóstico no LH, foi estudada a expressão do IMP3 em 61 casos, incluindo estratificação da imunocoloração em intensidades (0, 1+, 2+ e 3+) e relação com sobrevida, dados clínicos, aspectos morfológicos e imuno-histoquímicos. A expressão forte (intensidade 3+) para IMP3 foi observada em 61% (34/56) dos LH e apresentou forte indicio de associação com melhor sobrevida livre de doença. Nossos achados mostram o IMP3 como potencial biomarcador de melhor prognóstico em LH
Hodgkin lymphoma (HL) is a hematopoietic neoplasm that characteristically affects young adults and, if untreated, presents a fatal clinical course. Early and accurate diagnosis of this lymphoma associated with modern therapeutic management, on the other hand, has promoted a dramatic decrease in mortality rates. Despite this, around 15-20% of patients show unsatisfactory progression, with resistance to primary therapy or early relapse following treatment. The literature comprises numerous studies attempting to determine prognostic profiles that are capable of selecting candidate patients for specific therapies. Insulin-like growth factor mRNA binding protein 3 (IMP3) has been described as a biomarker that indicates worse prognosis in several malignancies, particularly carcinomas; however, studies of IMP3 in hematopoietic malignancies are rare. To assess their potential prognostic role in HL, IMP3 expression was studied in 61 cases and included stratification of immunostaining intensities (0, +, ++ and +++) and their relation to status of life, clinical data and morphological and immunohistochemical features. Intense IMP3 expression (+++) was observed in 61% (34/56) cases and showed a strong indication of association with better disease-free survival. Our findings show that IMP3 is a potential biomarker of better prognosis in HL
9
Moraes, Marcelo Padovani de Toledo. "Linfoma de Hodgkin : características anátomo-clínicas e análise de novo biomarcador (IMP3) /." Botucatu, 2015. http://hdl.handle.net/11449/132038.
Full textAbstract:
Orientador: Maria Aparecida Custódio DominguesBanca: Flávio de Oliveira Lima
Banca: Gabriela Gualco
Resumo: O Linfoma de Hodgkin (LH) é uma neoplasia hematopoiética que acomete caracteristicamente adultos jovens e apresenta, quando não tratado, curso clínico fatal. O diagnóstico precoce e preciso desse linfoma, associado à terapia moderna, por outro lado, tem proporcionado diminuição dramática das taxas de letalidade. Ainda assim, cerca de 15-20% dos pacientes evoluem de maneira insatisfatória com resistência a terapia primária ou recaída precoce pós-tratamento. Nesse sentido, há estudos variados na tentativa de buscar perfis prognósticos capazes de selecionar pacientes candidatos a terapias particulares. O IMP3 (insulin-like growth factor mRNA binding protein 3) tem sido descrito como biomarcador relacionado a pior prognóstico em diversas neoplasias, especialmente carcinomas, mas ainda pouco estudado em neoplasias hematopoiéticas. Para avaliar seu potencial papel prognóstico no LH, foi estudada a expressão do IMP3 em 61 casos, incluindo estratificação da imunocoloração em intensidades (0, 1+, 2+ e 3+) e relação com sobrevida, dados clínicos, aspectos morfológicos e imuno-histoquímicos. A expressão forte (intensidade 3+) para IMP3 foi observada em 61% (34/56) dos LH e apresentou forte indicio de associação com melhor sobrevida livre de doença. Nossos achados mostram o IMP3 como potencial biomarcador de melhor prognóstico em LH
Abstract: Hodgkin lymphoma (HL) is a hematopoietic neoplasm that characteristically affects young adults and, if untreated, presents a fatal clinical course. Early and accurate diagnosis of this lymphoma associated with modern therapeutic management, on the other hand, has promoted a dramatic decrease in mortality rates. Despite this, around 15-20% of patients show unsatisfactory progression, with resistance to primary therapy or early relapse following treatment. The literature comprises numerous studies attempting to determine prognostic profiles that are capable of selecting candidate patients for specific therapies. Insulin-like growth factor mRNA binding protein 3 (IMP3) has been described as a biomarker that indicates worse prognosis in several malignancies, particularly carcinomas; however, studies of IMP3 in hematopoietic malignancies are rare. To assess their potential prognostic role in HL, IMP3 expression was studied in 61 cases and included stratification of immunostaining intensities (0, +, ++ and +++) and their relation to status of life, clinical data and morphological and immunohistochemical features. Intense IMP3 expression (+++) was observed in 61% (34/56) cases and showed a strong indication of association with better disease-free survival. Our findings show that IMP3 is a potential biomarker of better prognosis in HL
Mestre
10
Ocanha, Juliana Polizel. "FOXP3 e IMP3 impacto na evolução dos diferentes subtipos clínicos de melanoma cutâneo /." Botucatu, 2016. http://hdl.handle.net/11449/143969.
Full textAbstract:
Orientador: Mariângela Esther Alencar MarquesResumo: INTRODUÇÃO: O melanoma apresentou aumento na sua incidência com o passar dos anos. A despeito das melhorias no diagnóstico e tratamento, demonstra mortalidade considerável. A incapacidade de prever com maior precisão sua evolução ainda intriga os pesquisadores. Dois marcadores imuno-histoquímicos, FOXP3 e IMP3 vêm sendo relacionados a pior prognóstico em vários estudos. OBJETIVO: Avaliar se a presença de FOXP3 e IMP3 é diferente entre os subtipos clínicos e se há correlação entre a sua presença e pior prognóstico. MÉTODOS: Estudo do tipo coorte retrospectiva, que avaliou todos os pacientes diagnosticados com Melanoma a partir do exame anatomopatológico no período de 2003 a 2011 provenientes dos serviços de Dermatologia e Patologia da Faculdade de Medicina de Botucatu (UNESP). As lâminas foram reanalisadas por dois patologistas e uma dermatologista, para assegurar subtipo clinico, Breslow, presença de ulceração, mitoses e regressão histológica. A partir dos blocos mantidos em arquivo se realizou a técnica imuno-histoquímica dos marcadores FOXP3 e IMP3. Além disso, foi realizado estudo de prontuário para avaliar aspectos demográficos, clínicos e de evolução do paciente. Foram aplicados os testes Qui-Quadrado ou Exato de Fisher, Kruskall Wallis, Teste de Dunn e Modelo de Regressão de Cox. Foi considerado estatisticamente significante p<0,05. RESULTADOS: A maioria de casos teve positividade < ou = a 25% tanto para IMP3 como para FOXP3. A positividade deles não pôde ser correlacio... (Resumo completo, clicar acesso eletrônico abaixo)
Mestre
Books on the topic "Imp3p"
1
Malaysia. Kementerian Perdagangan Antarabangsa & Industri., ed. Third industrial master plan, 2006-2020: Malaysia, towards global competitiveness : IMP3. Kuala Lumpur, Malaysia: Ministry of International Trade and Industry, Malaysia, 2006.
Find full text
2
Lenz, Georg. 2. Staatsexame Medizin. Alle Fakten aus den IMPP-Prüfungen. Wissenschaftliche Verlagsges., 2002.
Find full textBook chapters on the topic "Imp3p"
1
Culpeper, Jonathan. "Impoliteness." In Handbook of Pragmatics, 1–18. Amsterdam: John Benjamins Publishing Company, 2013. http://dx.doi.org/10.1075/hop.17.imp3.
Full text
2
J., Mark, and Haodong Xu. "IMP3 and Malignant Melanoma." In Advances in Malignant Melanoma - Clinical and Research Perspectives. InTech, 2011. http://dx.doi.org/10.5772/20219.
Full text
3
"A IMPP-Gegenstandskatalog für den schriftlichen Teil des Zweiten Abschnitts der Ärztlichen Prüfung." In Kompaktleitfaden Medizin 2011/2012, 1. Berlin, New York: DE GRUYTER, 2011. http://dx.doi.org/10.1515/9783110265583.1.
Full textConference papers on the topic "Imp3p"
1
Yin, Xiaolei, and Wei Chen. "Enhanced Sequential Optimization and Reliability Assessment Method for Changing Design Variance." In ASME 2005 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/detc2005-84891.
Full textAbstract:
The Sequential Optimization and Reliability Assessment (SORA) method is a single loop method containing a sequence of cycles of decoupled deterministic optimization and reliability assessment for improving the efficiency of probabilistic optimization. However, the original SORA method as well as some other existing single loop methods is not efficient for solving problems with changing variance. In this paper, to enhance the SORA method, three formulations are proposed by taking the effect of changing variance into account. These formulations are distinguished by the different strategies of Inverse Most Probable Point (IMPP) approximation. Mathematical examples and a pressure vessel design problem are used to test and compare the effectiveness of the proposed formulations. The “Direct Linear Estimation Formulation” is shown to be the most effective and efficient approach for dealing with problems with changing variance. The gained insight can be extended and utilized to other optimization strategies that require MPP or IMPP estimations.
2
IlJin Lee, Wook Hyun, and ShinGak Kang. "A study on user data management for SIP-based IMPP services." In 8th International Conference on Advanced Communication Technology. IEEE, 2006. http://dx.doi.org/10.1109/icact.2006.206315.
Full text
3
Sun Ok Park, Il Jin Lee, Jae Cheon Han, Mi-Young Huh, and Shin Gak Kang. "A study on XCAP client system for SIP-based IMPP services." In The 7th International Conference on Advanced Communication Technology. IEEE, 2005. http://dx.doi.org/10.1109/icact.2005.246134.
Full text
4
Joshi, Amitabh, and Min Xiao. "Cavity quantum electrodynamics with quantum interference in a three-level atomic system." In International Quantum Electronics Conference. Washington, D.C.: OSA, 2004. http://dx.doi.org/10.1364/iqec.2004.imp3.
Full text
5
IlJin Lee, SunOk Park, Wook Hyun, and ShinGak Kang. "A study on buddy list control in XCAP server system for SIP-based IMPP services." In The 7th International Conference on Advanced Communication Technology. IEEE, 2005. http://dx.doi.org/10.1109/icact.2005.246133.
Full text
6
Dhole, Rajaram, Ismael Ripoll, Sabesan Rajaratnam, and Celine Jablonski. "Effect of Temperature, Reeling Speed and Pipe Tension on the Performance of Field Joint Coating During Reeling of Offshore Pipelines." In Offshore Technology Conference. OTC, 2021. http://dx.doi.org/10.4043/30944-ms.
Full textAbstract:
Abstract Pipelines are coated with insulating material that minimizes heat losses to the environment. Reeled pipe can experience nominal bending strain in the order of 1% to 2%. Thick coating on the pipe is inherently more highly strained, because of concentrations that occur at the interface between parent coating and field joint coating. Occasionally, contractors who specialize in pipe-lay using the reeling method have experienced difficulties relating to unexpected disbondment and cracks in coating at these interfaces. Any disbonded coating is routinely identified and repaired, but it is important to understand the influential factors that could lead to this type of coating disbondment. It is known in the industry that parameters such as temperature, reeling speed and pipe tension are influential but the relative influence of the factors is not well understood. In addition, there is currently no industry code or recommended practice that proposes the strain levels that the coating could safely withstand prior to cracking. This paper addresses thermo-mechanical aspects of coating design and presents a novel approach to quantify which parameters have the largest influence. In the presented assessments, coating strain was assessed using finite element analysis. Material input was selected from a combination of typical values and specific laboratory test results for polypropylene (PP) and injection molded polypropylene (IMPP). An essential aspect was that the mechanical and thermal properties of the PP were related to temperature and strain rate. Strain rates in the coating during reeling operations were obtained from global FE models. Detailed local FE models incorporated all the material and load inputs and temperature conditions that are necessary to determine peak strain values in the coating; the peak strain values would indicate the locations of potential coating disbondment. The study is purely a strain assessment and excludes any potential for defects or delamination in the coating that could result from its manufacturing process. This strain-based study revealed that coating temperature during reeling is the most influential factor on strain level in the coating. Reeling speed and pipe tension are parameters providing secondary influences.