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Journal articles on the topic 'In-Cell EPR'

Author: Grafiati

Published: 25 May 2024

Last updated: 31 July 2025

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1

Adida, C., G. Ambrosini, J. Plescia, PL Crotty, J. Costa, and DC Altieri. "Protease receptors in Hodgkin's disease: expression of the factor Xa receptor, effector cell protease receptor-1, in Reed-Sternberg cells." Blood 88, no. 4 (1996): 1457–64. http://dx.doi.org/10.1182/blood.v88.4.1457.bloodjournal8841457.

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The expression of a cellular receptor for the blood-clotting protease factor Xa, designated effector cell protease receptor-1 (EPR-1), was investigated in lymphoma. Immunohistochemical analysis demonstrated prominent reactivity of monoclonal antibodies to EPR-1 with Reed- Sternberg cells in 30 of 35 cases of nodular-sclerosis, lymphocyte- depletion, and mixed-cellularity Hodgkin's disease (HD). In contrast, several non-Hodgkin's lymphomas, or the nonneoplastic cellular components of HD, did not react with anti-EPR-1 monoclonal antibodies. A single molecular species of approximately 62 kD, cons

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2

Cattani, Julia, Vinod Subramaniam, and Malte Drescher. "Room-temperature in-cell EPR spectroscopy: alpha-Synuclein disease variants remain intrinsically disordered in the cell." Physical Chemistry Chemical Physics 19, no. 28 (2017): 18147–51. http://dx.doi.org/10.1039/c7cp03432f.

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3

Zaman, Guido J. R., and Edward M. Conway. "The elusive factor Xa receptor: failure to detect transcripts that correspond to the published sequence of EPR-1." Blood 96, no. 1 (2000): 145–48. http://dx.doi.org/10.1182/blood.v96.1.145.

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Abstract The coagulation protease factor Xa induces cellular responses implicated in cardiovascular and inflammatory disease. Effector-cell protease receptor 1 (EPR-1) is a functionally characterized receptor of factor Xa, and the EPR-1complementary DNA (cDNA) was published. Remarkably, the cDNA encoding an inhibitor of apoptosis, survivin, is reportedly identical to that ofEPR-1 except for a few nucleotide differences and its orientation opposite to EPR-1. To isolate the EPR-1cDNA and gene, we surveyed gene databases for expressed sequence tags (ESTs) that could be derived from EPR-1. All EST

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4

Zaman, Guido J. R., and Edward M. Conway. "The elusive factor Xa receptor: failure to detect transcripts that correspond to the published sequence of EPR-1." Blood 96, no. 1 (2000): 145–48. http://dx.doi.org/10.1182/blood.v96.1.145.013k34_145_148.

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The coagulation protease factor Xa induces cellular responses implicated in cardiovascular and inflammatory disease. Effector-cell protease receptor 1 (EPR-1) is a functionally characterized receptor of factor Xa, and the EPR-1complementary DNA (cDNA) was published. Remarkably, the cDNA encoding an inhibitor of apoptosis, survivin, is reportedly identical to that ofEPR-1 except for a few nucleotide differences and its orientation opposite to EPR-1. To isolate the EPR-1cDNA and gene, we surveyed gene databases for expressed sequence tags (ESTs) that could be derived from EPR-1. All ESTs with st

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5

Hänsel, Robert, Laura M. Luh, Ivan Corbeski, Lukáš Trantirek, and Volker Dötsch. "In-Cell NMR and EPR Spectroscopy of Biomacromolecules." Angewandte Chemie International Edition 53, no. 39 (2014): 10300–10314. http://dx.doi.org/10.1002/anie.201311320.

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6

Ovcherenko, Sergey S., Olga A. Chinak, Anton V. Chechushkov, et al. "Uptake of Cell-Penetrating Peptide RL2 by Human Lung Cancer Cells: Monitoring by Electron Paramagnetic Resonance and Confocal Laser Scanning Microscopy." Molecules 26, no. 18 (2021): 5442. http://dx.doi.org/10.3390/molecules26185442.

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RL2 is a recombinant analogue of a human κ-casein fragment, capable of penetrating cells and inducing apoptosis of cancer cells with no toxicity to normal cells. The exact mechanism of RL2 penetration into cells remains unknown. In this study, we investigated the mechanism of RL2 penetration into human lung cancer A549 cells by a combination of electron paramagnetic resonance (EPR) spectroscopy and confocal laser scanning microscopy. EPR spectra of A549 cells incubated with RL2 (sRL2) spin-labeled by a highly stable 3-carboxy-2,2,5,5-tetraethylpyrrolidine-1-oxyl radical were found to contain t

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7

Piknova, Barbora, Mark T. Gladwin, Cheryl A. Hillery, and Neil Hogg. "Electron Paramagnetic Resonance Study of Cell-Free Hemoglobin in Sickle Cell Disease: Potential Antioxidant Role of Haptoglobin." Blood 106, no. 11 (2005): 2345. http://dx.doi.org/10.1182/blood.v106.11.2345.2345.

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Abstract It has previously been shown that red blood cell-free oxyhemoglobin (oxyHb) can affect endothelial function in Sickle Cell Disease (SCD) by virtue of its NO scavenging properties (Reiter et al, Nat Med, 2002). This reaction may have down-stream consequence via formation of metHb which has been demonstrated to have pro-oxidant properties and can oxidize low-density lipoprotein in vitro. In this study we have examined the fate of metHb in SCD vs normal plasma by examining the characteristic single line EPR signature of metHb in the g = 6 region. We show that the EPR signature of metHb d

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8

Damen, J., AL Mui, P. Hughes, K. Humphries, and G. Krystal. "Erythropoietin-induced tyrosine phosphorylations in a high erythropoietin receptor-expressing lymphoid cell line." Blood 80, no. 8 (1992): 1923–32. http://dx.doi.org/10.1182/blood.v80.8.1923.1923.

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Abstract Retroviral gene transfer of the murine erythropoietin receptor (EpR) cDNA into the pro-B-cell line, Ba/F3, was used to generate cells expressing high EpR levels. One of the resulting clones, Ba/F3 clone C5, contained 5 integrated copies of the gene and expressed, at the cell surface, a single affinity class of EpRs at 10 to 15 times the level present on spleen cells from phenylhydrazine-treated mice. Cross- linking studies with clone C5, using 125I-Ep, yielded the same two 105- and 88-Kd major species as that seen with typical erythroid cells. This was distinct from that obtained with

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9

Damen, J., AL Mui, P. Hughes, K. Humphries, and G. Krystal. "Erythropoietin-induced tyrosine phosphorylations in a high erythropoietin receptor-expressing lymphoid cell line." Blood 80, no. 8 (1992): 1923–32. http://dx.doi.org/10.1182/blood.v80.8.1923.bloodjournal8081923.

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Retroviral gene transfer of the murine erythropoietin receptor (EpR) cDNA into the pro-B-cell line, Ba/F3, was used to generate cells expressing high EpR levels. One of the resulting clones, Ba/F3 clone C5, contained 5 integrated copies of the gene and expressed, at the cell surface, a single affinity class of EpRs at 10 to 15 times the level present on spleen cells from phenylhydrazine-treated mice. Cross- linking studies with clone C5, using 125I-Ep, yielded the same two 105- and 88-Kd major species as that seen with typical erythroid cells. This was distinct from that obtained with EpR-tran

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10

Bonucci, Alessio, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, and Elisabetta Mileo. "In‐Cell EPR: Progress towards Structural Studies Inside Cells." ChemBioChem 21, no. 4 (2019): 451–60. http://dx.doi.org/10.1002/cbic.201900291.

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11

Kranenburg, Onno, Benjamin L. Emmink, Jaco Knol, Winan J. van Houdt, Inne HM Borel Rinkes, and Connie R. Jimenez. "Proteomics in studying cancer stem cell biology." Expert Review of Proteomics 9, no. 3 (2012): 325–36. http://dx.doi.org/10.1586/epr.12.24.

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12

Widder, Pia, Julian Schuck, Daniel Summerer, and Malte Drescher. "Combining site-directed spin labeling in vivo and in-cell EPR distance determination." Physical Chemistry Chemical Physics 22, no. 9 (2020): 4875–79. http://dx.doi.org/10.1039/c9cp05584c.

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13

Czejka, Martin, Suzan Bandak, Doris Simon, Johann Schiiller, Claudia Weiss, and Eva Schernhammer. "Pharmacokinetic Interaction between 4′-Epidoxorubicin and the Multidrug Resistance Reverting Agent Quinine." Zeitschrift für Naturforschung C 50, no. 7-8 (1995): 565–70. http://dx.doi.org/10.1515/znc-1995-7-815.

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The serum and red blood cell (RBCs) disposition of epirubicin (EPR) after intravenous bolus injection without and with coadministered quinine (QUI) was investigated in patients undergoing a cyclic chemotherapy with EPR. QUI possesses a statistical significant influence on the EPR serum concentrations and, as a consequence, on the pharmacokinetic parameters for the initial distribution phase of EPR. Within the first 15 min after administration, EPR was distributed from the central compartiment distinctly faster in compare to the control, when QUI was preadministered (t1/2 = 6 min for the contro

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14

Key, Fiona B., Devina McClean, and J. C. Mathers. "Tissue hypertrophy and epithelial proliferation rate in the gut of rats fed on bread and haricot beans (Phaseolus vulgaris)." British Journal of Nutrition 76, no. 2 (1996): 273–86. http://dx.doi.org/10.1079/bjn19960031.

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The present study was designed to test the hypothesis that increasing short-chain fatty acid (SCFA)production in the large bowel increases gut epithelial proliferation rate (EPR). Two experiments were carried out in which rats were fed on bread (wholemeal or white)-based diets containing graded amounts of cooked haricot (Phaseofus vulgaris) beans; the latter are a rich source of fermentable carbohydrates. Consumption of beans was associated with several-fold increases in SCFA production with the greatest relative increase being for butyrate. Despite the very large increase in SCFA production,

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15

Jegdić, Bore V., and Biljana M. Bobić. "Determination of susceptibility to intergranular corrosion of stainless steels type X5CrNi18-10 in field." Metallurgical and Materials Engineering 22, no. 4 (2016): 251–60. http://dx.doi.org/10.30544/236.

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In this paper, the DL EPR method (electrochemical potentiokinetic reactivation with double loop) was modified and used to study the susceptibility to intergranular corrosion and stress corrosion cracking of a stainless steel type X5CrNi18-10. The tests were performed in a special electrochemical cell, with the electrolyte in the gel form. Modified DL EPR method is characterized by simple and high accuracy measurements as well as repeatability of the test results. The indicator of susceptibility to intergranular corrosion (Qr/Qp)GBA obtained by modified DL EPR method is in a very good agreement

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16

Cangiotti, Michela, Sara Salucci, Michela Battistelli, et al. "EPR, TEM and cell viability study of asbestiform zeolite fibers in cell media." Colloids and Surfaces B: Biointerfaces 161 (January 2018): 147–55. http://dx.doi.org/10.1016/j.colsurfb.2017.10.045.

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17

Krosl, J., JE Damen, G. Krystal, and RK Humphries. "Erythropoietin and interleukin-3 induce distinct events in erythropoietin receptor-expressing BA/F3 cells." Blood 85, no. 1 (1995): 50–56. http://dx.doi.org/10.1182/blood.v85.1.50.bloodjournal85150.

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To compare the signal transduction pathways used by erythropoietin (Ep) and interleukin-3 (IL-3), the cDNA for the murine erythropoietin receptor (EpR) was introduced into the IL-3-responsive cell lines Ba/F3 and DA-3 using retrovirally mediated gene transfer. After selection in G418 and IL-3, clones expressing comparable levels of cell surface EpR were identified using biotinylated Ep and flow cytometry. A comparison of the effects of Ep and IL-3 on these cells showed that most EpR+ Ba/F3 clones, when first exposed to Ep, dramatically increased their levels of beta-globin mRNA. The kinetics o

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18

Kucher, Svetlana, Sergej Korneev, Johann P. Klare, Daniel Klose, and Heinz-Jürgen Steinhoff. "In cell Gd3+-based site-directed spin labeling and EPR spectroscopy of eGFP." Physical Chemistry Chemical Physics 22, no. 24 (2020): 13358–62. http://dx.doi.org/10.1039/d0cp01930e.

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19

Qi, Mian, Andreas Groß, Gunnar Jeschke, Adelheid Godt, and Malte Drescher. "Gd(III)-PyMTA Label Is Suitable for In-Cell EPR." Journal of the American Chemical Society 136, no. 43 (2014): 15366–78. http://dx.doi.org/10.1021/ja508274d.

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20

Holder, Isabelle T., Malte Drescher, and Jörg S. Hartig. "Structural characterization of quadruplex DNA with in-cell EPR approaches." Bioorganic & Medicinal Chemistry 21, no. 20 (2013): 6156–61. http://dx.doi.org/10.1016/j.bmc.2013.04.014.

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21

Haensel, Robert, Laura M. Luh, Ivan Corbeski, Lukas Trantirek, and Volker Doetsch. "ChemInform Abstract: In-Cell NMR and EPR Spectroscopy of Biomacromolecules." ChemInform 45, no. 49 (2014): no. http://dx.doi.org/10.1002/chin.201449278.

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22

Brown, Kristy J., Catherine A. Formolo, Haeri Seol, et al. "Advances in the proteomic investigation of the cell secretome." Expert Review of Proteomics 9, no. 3 (2012): 337–45. http://dx.doi.org/10.1586/epr.12.21.

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23

Sang, Xiancheng, Xixiang Xu, Zeyuan Bu, et al. "Application of Electron Paramagnetic Resonance in an Electrochemical Energy Storage System." Magnetochemistry 9, no. 3 (2023): 63. http://dx.doi.org/10.3390/magnetochemistry9030063.

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The improvement of our living standards puts forward higher requirements for energy storage systems, especially rechargeable batteries. Unfortunately, phenomena such as capacity failure, etc. have been major difficulties in the field of energy storage. Therefore, we need some advanced means to explore the reaction process and mechanisms of the cell. Electron paramagnetic resonance (EPR) has the advantages of a high sensitivity to electrons, lack of damage to samples, quantitative analysis, etc., which can make for a more in-depth exploration of most paramagnetic electrode materials and metal e

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24

Damen, JE, AL Mui, L. Puil, T. Pawson, and G. Krystal. "Phosphatidylinositol 3-kinase associates, via its Src homology 2 domains, with the activated erythropoietin receptor." Blood 81, no. 12 (1993): 3204–10. http://dx.doi.org/10.1182/blood.v81.12.3204.3204.

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Abstract The erythropoietin receptor (EpR) belongs to a family of hematopoietin receptors whose members lack tyrosine kinase activity. Nonetheless, within minutes of binding Ep, a number of cellular proteins become transiently phosphorylated on tyrosine residues. One of these proteins, as we and others have shown previously, is the EpR itself. To identify the remaining protein substrates, we have examined the antiphosphotyrosine immunoprecipitates of lysates from Ba/F3 cells expressing high levels of cell surface EpRs. We now present data showing that, in response to Ep, the 85-Kd regulatory s

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25

Damen, JE, AL Mui, L. Puil, T. Pawson, and G. Krystal. "Phosphatidylinositol 3-kinase associates, via its Src homology 2 domains, with the activated erythropoietin receptor." Blood 81, no. 12 (1993): 3204–10. http://dx.doi.org/10.1182/blood.v81.12.3204.bloodjournal81123204.

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The erythropoietin receptor (EpR) belongs to a family of hematopoietin receptors whose members lack tyrosine kinase activity. Nonetheless, within minutes of binding Ep, a number of cellular proteins become transiently phosphorylated on tyrosine residues. One of these proteins, as we and others have shown previously, is the EpR itself. To identify the remaining protein substrates, we have examined the antiphosphotyrosine immunoprecipitates of lysates from Ba/F3 cells expressing high levels of cell surface EpRs. We now present data showing that, in response to Ep, the 85-Kd regulatory subunit of

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26

Zhelev, Zhivko, Ekaterina Georgieva, Dessislava Lazarova, et al. "“Redox Imaging” to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers." Oxidative Medicine and Cellular Longevity 2019 (April 8, 2019): 1–18. http://dx.doi.org/10.1155/2019/6373685.

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The present study was directed to the development of EPR methodology for distinguishing cells with different proliferative activities, using “redox imaging.” Three nitroxide radicals were used as redox sensors: (a) mito-TEMPO—cell-penetrating and localized mainly in the mitochondria; (b) methoxy-TEMPO—cell-penetrating and randomly distributed between the cytoplasm and the intracellular organelles; and (c) carboxy-PROXYL—nonpenetrating in living cells and evenly distributed in the extracellular environment. The experiments were conducted on eleven cell lines with different proliferative activit

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27

Sabetghadam Moghadam, Mitra, Eli Wiens, Sébastien Gauvrit, Ramaswami Sammynaiken, and Michelle M. Collins. "Electron paramagnetic resonance spectroscopy for analysis of free radicals in zebrafish." PLOS ONE 20, no. 2 (2025): e0318212. https://doi.org/10.1371/journal.pone.0318212.

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Electron paramagnetic resonance (EPR) is an excellent choice for detecting free radicals in biological samples. Biologically relevant radicals are extremely short-lived and cannot be detected directly, emphasizing the need for an appropriate compound to generate stable adducts that can be measured by EPR. Spin trapping with nitrone compounds like 5,5-dimethyl-1-pyrroline N-oxide (DMPO) is a method commonly employed for detecting free radicals. However, due to the instability of nitrone radical adducts, using the cell-permeable 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethyl pyrrolidine (CMH) a

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28

Ando, Takahiro, and Yoshiki Yonamoto. "Cell Discrimination Using In-Situ EPR Measurement of Reactive Oxygen Species." Free Radical Biology and Medicine 87 (October 2015): S108. http://dx.doi.org/10.1016/j.freeradbiomed.2015.10.283.

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29

Goldfarb, Daniella. "Exploring protein conformations in vitro and in cell with EPR distance measurements." Current Opinion in Structural Biology 75 (August 2022): 102398. http://dx.doi.org/10.1016/j.sbi.2022.102398.

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30

Zapparoli, Ettore, Paola Briata, Martina Rossi, Lorenzo Brondolo, Gabriele Bucci та Roberto Gherzi. "Comprehensive multi-omics analysis uncovers a group of TGF-β-regulated genes among lncRNA EPR direct transcriptional targets". Nucleic Acids Research 48, № 16 (2020): 9053–66. http://dx.doi.org/10.1093/nar/gkaa628.

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Abstract Long non-coding RNAs (lncRNAs) can affect multiple layers of gene expression to control crucial cellular functions. We have previously demonstrated that the lncRNA EPR, by controlling gene expression at different levels, affects cell proliferation and migration in cultured mammary gland cells and impairs breast tumor formation in an orthotopic transplant model in mice. Here, we used ChIRP-Seq to identify EPR binding sites on chromatin of NMuMG mammary gland cells overexpressing EPR and identified its trans binding sites in the genome. Then, with the purpose of relating EPR/chromatin i

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31

Gowthaman, Uthaman, and Javed N. Agrewala. "In silicomethods for predicting T-cell epitopes: Dr Jekyll or Mr Hyde?" Expert Review of Proteomics 6, no. 5 (2009): 527–37. http://dx.doi.org/10.1586/epr.09.71.

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32

Boś-Liedke, Agnieszka, Magdalena Walawender, Anna Woźniak, et al. "EPR Oximetry Sensor—Developing a TAM Derivative for In Vivo Studies." Cell Biochemistry and Biophysics 76, no. 1-2 (2017): 19–28. http://dx.doi.org/10.1007/s12013-017-0824-3.

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Abstract Oxygenation is one of the most important physiological parameters of biological systems. Low oxygen concentration (hypoxia) is associated with various pathophysiological processes in different organs. Hypoxia is of special importance in tumor therapy, causing poor response to treatment. Triaryl methyl (TAM) derivative radicals are commonly used in electron paramagnetic resonance (EPR) as sensors for quantitative spatial tissue oxygen mapping. They are also known as magnetic resonance imaging (MRI) contrast agents and fluorescence imaging compounds. We report the properties of the TAM

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33

Czekański, Łukasz, Stanisław K. Hoffmann, Piotr Barczyński, et al. "Crystal structure and physical properties of 1-methyl-3-(carboxymethyl)benzimidazolium betaine·CuBr2 in crystal and water solution." New Journal of Chemistry 40, no. 12 (2016): 10526–35. http://dx.doi.org/10.1039/c6nj03192g.

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34

Barham, R. J., and D. C. Doetschman. "Single crystal electron paramagnetic resonance study of Y2BaCuO5, a common impurity in the high temperature superconductor, YBa2Cu3O7." Journal of Materials Research 7, no. 3 (1992): 565–71. http://dx.doi.org/10.1557/jmr.1992.0565.

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Electron paramagnetic resonance (EPR) studies of pure Y2BaCuO5 in powder and single crystal forms and of YBa2Cu3O7−δ in powder and single crystal forms provide further evidence that it is Y2BaCuO5 that is the common green impurity found in many preparations of YBa2Cu3O7−δ as a powder or in pellet forms. Y2BaCuO5 tends to be excluded in the growth of YBa2Cu3O7−δ single crystals. A method is presented for the growth of Y2BaCuO5 crystals from a flux. An apparent discrepancy between the observed single crystal EPR anisotropy and the reported crystal structure is resolved in three independent ways

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35

Levasseur-Acker, Germaine, Roger Zalma, Edith Copin, Jeanine Fournier, Henri Pezerat, and Roger Jankowski. "Peroxydation de l'acide linolénique en présence des fibres d'amiante ou de némalite. Résultats préliminaires avec des cellules épithéliales." Canadian Journal of Chemistry 73, no. 3 (1995): 453–59. http://dx.doi.org/10.1139/v95-059.

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Linolenic acid (LH) peroxidation in aqueous medium is studied in the presence of fibers of chrysotile (UICC A and B), crocidolite UICC, and némalite. Preliminary studies on the peroxydation of epithelial cell membranes were also performed. Radicals (L•, LOO•) formed from the peroxidation process are detected by EPR with a spin trapping agent, POBN. The activity of these iron(II)-containing fibers is related to the formation of activated oxygen species, particularly of some highly electrophilic species (•OH or perferryl and ferryl entities). The presence of these species is shown by EPR with a

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36

Santucci, Roberto, Federica Sinibaldi, Antonella Patriarca, Daniele Santucci, and Laura Fiorucci. "Misfolded proteins and neurodegeneration: role of non-native cytochrome c in cell death." Expert Review of Proteomics 7, no. 4 (2010): 507–17. http://dx.doi.org/10.1586/epr.10.50.

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37

Boggio, Kristin J., Emmanuel Obasuyi, Ken Sugino, Sacha B. Nelson, Nathalie YR Agar, and Jeffrey N. Agar. "Recent advances in single-cell MALDI mass spectrometry imaging and potential clinical impact." Expert Review of Proteomics 8, no. 5 (2011): 591–604. http://dx.doi.org/10.1586/epr.11.53.

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38

Kegulian, Natalie C., Ralf Langen, and Janet Moradian-Oldak. "The Dynamic Interactions of a Multitargeting Domain in Ameloblastin Protein with Amelogenin and Membrane." International Journal of Molecular Sciences 24, no. 4 (2023): 3484. http://dx.doi.org/10.3390/ijms24043484.

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The enamel matrix protein Ameloblastin (Ambn) has critical physiological functions, including regulation of mineral formation, cell differentiation, and cell–matrix adhesion. We investigated localized structural changes in Ambn during its interactions with its targets. We performed biophysical assays and used liposomes as a cell membrane model. The xAB2N and AB2 peptides were rationally designed to encompass regions of Ambn that contained self-assembly and helix-containing membrane-binding motifs. Electron paramagnetic resonance (EPR) on spin-labeled peptides showed localized structural gains

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39

Murray, Paul R., David Collison, Simon Daff, et al. "An in situ electrochemical cell for Q- and W-band EPR spectroscopy." Journal of Magnetic Resonance 213, no. 1 (2011): 206–9. http://dx.doi.org/10.1016/j.jmr.2011.09.041.

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40

Moreira, Rodrigo Alves, Sebastião Antonio Mendanha, Kelly Souza Fernandes, et al. "Miltefosine Increases Lipid and Protein Dynamics in Leishmania amazonensis Membranes at Concentrations Similar to Those Needed for Cytotoxicity Activity." Antimicrobial Agents and Chemotherapy 58, no. 6 (2014): 3021–28. http://dx.doi.org/10.1128/aac.01332-13.

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ABSTRACTMiltefosine (MT) is a membrane-active alkylphospholipid licensed for the topical treatment of breast cancer skin metastases and the oral treatment of leishmaniasis, although its mechanism of action remains unclear. Electron paramagnetic resonance (EPR) spectroscopy of a spin-labeled lipid and a thiol-specific spin label in the plasma membrane ofLeishmaniapromastigotes showed that MT causes dramatic increases in membrane dynamics. Although these alterations can be detected using a spin-labeled lipid, our experimental results indicated that MT interacts predominantly with the protein com

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41

Panchenko, A., H. Dilger, E. Möller, T. Sixt, and E. Roduner. "In situ EPR investigation of polymer electrolyte membrane degradation in fuel cell applications." Journal of Power Sources 127, no. 1-2 (2004): 325–30. http://dx.doi.org/10.1016/j.jpowsour.2003.09.047.

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42

Glatzhofer, Daniel T., and Rahul S. Kadam. "Use of Electron Paramagnetic Resonance Spectroscopy to Study Dielectric Properties of Liquids." ISRN Analytical Chemistry 2012 (April 9, 2012): 1–8. http://dx.doi.org/10.5402/2012/847102.

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The signal response of an EPR active species is attenuated by the medium it is in. Keeping all other parameters the same, the higher the dielectric constant of the medium, the lower the EPR signal response. This behavior is problematic in studying EPR active species in high dielectric media but can be capitalized upon to monitor changes in the dielectric constant or estimate the dielectric constant of the medium. Using a coaxial EPR cell design, the EPR signal of a stable nitroxyl radical compound (2,2,6,6-tetramethyl-piperidin-1-oxyl radical) in a low dielectric constant solvent in an inner t

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43

Alfei, Silvana, and Guendalina Zuccari. "One-Step, Low-Cost, Operator-Friendly, and Scalable Procedure to Synthetize Highly Pure N-(4-ethoxyphenyl)-retinamide in Quantitative Yield without Purification Work-Up." Molecules 27, no. 11 (2022): 3632. http://dx.doi.org/10.3390/molecules27113632.

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It is widely reported that N-(4-hydroxyphenyl)-retinamide or fenretinide (4-HPR), which is a synthetic amide of all-trans-retinoic acid (ATRA), inhibits in vitro several types of tumors, including cancer cell lines resistant to ATRA, at 1–10 µM concentrations. Additionally, studies in rats and mice have confirmed the potent anticancer effects of 4-HPR, without evidencing hemolytic toxicity, thus demonstrating its suitability for the development of a new chemo-preventive agent. To this end, the accurate determination of 4-HPR levels in tissues is essential for its pre-clinical training, and for

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Hartl, František, Ronald P. Groenestein, and Taasje Mahabiersing. "Air-Tight Three-Electrode Design of Coaxial Electrochemical-EPR Cell for Redox Studies at Low Temperatures." Collection of Czechoslovak Chemical Communications 66, no. 1 (2001): 52–66. http://dx.doi.org/10.1135/cccc20010052.

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The weak point of the original Allendoerfer electrochemical-EPR cell has been the reference electrode, placed outside the space-limited electrolysis cavity or not used at all in experiments at low temperatures. We present here an elegant solution to this problem, based on a modified air-tight design of an Allendoerfer cell equipped with a silver-wire pseudoreference electrode. The cell performance is demonstrated on one-electron electrochemical oxidation of heterocyclic 3,6-diphenyl-1,2-dithiine and one-electron reduction of 6-methyl-6-phenylfulvene and the pseudo-octahedral complex fac-[Re(be

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Vesković, Ana, Đura Nakarada, Aleksandra Pavićević, et al. "In Vivo/Ex Vivo EPR Investigation of the Brain Redox Status and Blood-Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease." Current Alzheimer Research 18, no. 1 (2021): 25–34. http://dx.doi.org/10.2174/1567205018666210324121156.

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Background: Alzheimer’s disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the research focus from Aβ to other pathological features of the disease. Objective: The aim of this study was to examine the interpl

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Wu, Jun. "The Enhanced Permeability and Retention (EPR) Effect: The Significance of the Concept and Methods to Enhance Its Application." Journal of Personalized Medicine 11, no. 8 (2021): 771. http://dx.doi.org/10.3390/jpm11080771.

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Chemotherapy for human solid tumors in clinical practice is far from satisfactory. Despite the discovery and synthesis of hundreds of thousands of anticancer compounds targeting various crucial units in cancer cell proliferation and metabolism, the fundamental problem is the lack of targeting delivery of these compounds selectively into solid tumor tissue to maintain an effective concentration level for a certain length of time for drug-tumor interaction to execute anticancer activities. The enhanced permeability and retention effect (EPR effect) describes a universal pathophysiological phenom

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Dutoit, Charles-Emmanuel, Raffaella Soleti, Jean-Marie Doux, et al. "Innovative L-band electron paramagnetic resonance investigation of solid-state pouch cell batteries." Magnetic Resonance 6, no. 1 (2025): 113–18. https://doi.org/10.5194/mr-6-113-2025.

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Abstract. Usually, conventional electron paramagnetic resonance (EPR) spectroscopy and imaging employ a microwave cavity operating at X-band, i.e., with an excitation frequency of around 9.6 GHz, and this remains the most popular mode for the magnetic characterization of lithium batteries to date. Here, we provide the first low-frequency EPR investigations with respect to monitoring the metallic lithium structures in solid-state pouch cell batteries. We show that L-band, i.e., a microwave frequency of around 1.01 GHz, is an invaluable method to probe the electrode components directly through a

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Wenzel, Barbara, and Peter Strauch. "Synthese, Struktur- und EPR-Untersuchungen an Bis(tetraphenylphosphonium)- bis(1,2-dithioquadratato)oxovanadat(IV), (Ph4P)2[VO(dtsq)2]/Synthesis, Structural and EPR Investigations on Bis(tetraphenylphosphonium)- bis(1,2-dithiosquarato)oxovanadate(IV), (Ph4P)2 [VO(dtsq)2]." Zeitschrift für Naturforschung B 54, no. 2 (1999): 165–70. http://dx.doi.org/10.1515/znb-1999-0203.

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The molecular structure and EPR studies of bis(tetraphenylphosphonium)bis( 1,2- dithiosquarato)oxovanadate(IV) are reported. (Ph4P)2[VO(dtsq)2] crystallizes in the monoclinic space group P21/n with the unit cell parameters a = 10,9820(2), b = 15,4620(3), c = 14,5050(3) Å, β = 95,700(8)°, Z = 2. The g and hyperfine coupling tensors Av obtained from the EPR spectra in liquid and frozen solution are used to characterize the properties of the molecular orbital containing the unpaired electron and are compared to those obtained from EHT-MO calculations.

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Geng, Fushan, Guozhong Lu, Yuxin Liao, Ming Shen, and Bingwen Hu. "Quantitative and space-resolved in situ 1D EPR imaging for the detection of metallic lithium deposits." Journal of Chemical Physics 157, no. 17 (2022): 174203. http://dx.doi.org/10.1063/5.0125080.

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The ability to monitor lithium deposition on the anodes in real time is becoming progressively more important due to the development of advanced anode technology. Given the fact that the detrimental Li deposits are always on the micron scale, electron paramagnetic resonance (EPR) happens to be a very effective and selective detection technology due to the skin effect. Here, quantitative in situ 1D EPR imaging is carried out with a magnetic field gradient to achieve a one-dimensional spatial resolution along the Li growth direction in a capillary cell. The quantification of Li deposits is caref

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Hsiang Kong, Darren Chyi, Kenneth Yew Choy Chew, Eng Lai Tan, and Suan Phaik Khoo. "Epiregulin (EPR) Reduces Epidermal Growth Factor (EGF) Receptor Expression In Oral Squamous Cell Carcinoma (OSCC) Cell Lines." Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 119, no. 3 (2015): e177. http://dx.doi.org/10.1016/j.oooo.2014.07.331.

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