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Journal articles on the topic 'In-situ hybridisation'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Herrington, C. S. "Demystified ... in situ hybridisation." Molecular Pathology 51, no. 1 (1998): 8–13. http://dx.doi.org/10.1136/mp.51.1.8.

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2

Cassidy, Andrew, and Julia Jones. "Developments in in situ hybridisation." Methods 70, no. 1 (2014): 39–45. http://dx.doi.org/10.1016/j.ymeth.2014.04.006.

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3

Warford, A., and I. Lauder. "In situ hybridisation in perspective." Journal of Clinical Pathology 44, no. 3 (1991): 177–81. http://dx.doi.org/10.1136/jcp.44.3.177.

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4

Ekong, Rosemary, and Jonathan Wolfe. "Advances in fluorescent in situ hybridisation." Current Opinion in Biotechnology 9, no. 1 (1998): 19–24. http://dx.doi.org/10.1016/s0958-1669(98)80079-7.

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5

Chiecchio, Laura. "In situ hybridisation in tissue sections." Diagnostic Histopathology 26, no. 11 (2020): 521–28. http://dx.doi.org/10.1016/j.mpdhp.2020.08.005.

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6

Charuruks, N. "In situ hybridisation in medical oncology." Chulalongkorn Medical Journal 40, no. 12 (1996): 1015–29. http://dx.doi.org/10.58837/chula.cmj.40.12.7.

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7

Yao, Jin, Xingmei Li, Na Wu, Songlin Zhang, Min Gao, and Xiping Wang. "Improvement of RNA In Situ Hybridisation for Grapevine Fruits and Ovules." International Journal of Molecular Sciences 24, no. 1 (2023): 800. http://dx.doi.org/10.3390/ijms24010800.

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The European grapevine (Vitis vinifera L.) is one of the world’s most widely cultivated and economically important fruit crops. Seedless fruits are particularly desired for table grapes, with seedlessness resulting from stenospermocarpy being an important goal for cultivar improvement. The establishment of an RNA in situ hybridisation (ISH) system for grape berries and ovules is, therefore, important for understanding the molecular mechanisms of ovule abortion in stenospermocarpic seedless cultivars. We improved RNA in situ hybridisation procedures for developing berries and ovules by targetin

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8

Edwards, A. A. "Editorial - Fluorescence In Situ Hybridisation (FISH)." Radiation Protection Dosimetry 88, no. 1 (2000): 5–6. http://dx.doi.org/10.1093/oxfordjournals.rpd.a033019.

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9

Morey, Adrienne L. "In situ hybridisation: background and applications." Pathology 43 (2011): S10. http://dx.doi.org/10.1016/s0031-3025(16)33100-2.

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10

Terenghi, Giorgio, and Julia M. Polak. "Morphological studies using in situ hybridisation." European Journal of Cancer and Clinical Oncology 27, no. 6 (1991): 785–89. http://dx.doi.org/10.1016/0277-5379(91)90190-o.

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11

Gill, Kamalvir, Jun Sasaki, Parul Jayakar, Lisa Sosa, and Elizabeth Welch. "Chromosomal microarray detects genetic risks of neurodevelopmental disorders in newborns with congenital heart disease." Cardiology in the Young 31, no. 8 (2021): 1275–82. http://dx.doi.org/10.1017/s1047951121000202.

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AbstractObjective:To compare the genetic testing results of neonates with CHD by chromosomal microarray to karyotyping and fluorescence in situ hybridisation analysis.Methods:This was a single-centre retrospective comparative study of patients with CHD and available genetic testing results admitted to the cardiac ICU between January, 2004 and December, 2017. Patients from 2004 to 2010 were tested by karyotyping and fluorescence in situ hybridisation analysis, while patients from 2012 to 2017 were analysed by chromosomal microarray.Results:Eight-hundred and forty-nine neonates with CHD underwen

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12

Shastry, Anjali, Preetha Tilak, and Amudha Subramaniam. "APPLICATION OF KARYOTYPING AND FLOURESCENT IN SITU HYBRIDISATION IN DETECTION OF KLINEFELTER SYNDROME." International Journal of Anatomy and Research 6, no. 3.3 (2018): 5682–85. http://dx.doi.org/10.16965/ijar.2018.310.

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13

Harrison, R. H., H. C. Kuo, P. N. Scriven, A. H. Handyside, and C. Mackie Ogilvie. "Lack of cell cycle checkpoints in human cleavage stage embryos revealed by a clonal pattern of chromosomal mosaicism analysed by sequential multicolour FISH." Zygote 8, no. 3 (2000): 217–24. http://dx.doi.org/10.1017/s0967199400001015.

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Multicolour fluorescence in situ hybridisation (FISH) analysis of interphase nuclei in cleavage stage human embryos has highlighted a high incidence of postzygotic chromosomal mosaicism, including both aneuploid and ploidy mosaicism. Indeed, some embryos appear to have a chaotic chromosomal complement in a majority of nuclei, suggesting that cell cycle checkpoints may not operate in early cleavage. Most of these studies, however, have only analysed a limited number of chromosomes (3–5), making it difficult to distinguish FISH artefacts from true aneuploidy. We now report analysis of 11 chromos

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14

Briscioli, V., G. Floridia, E. Rossi, A. Selicorni, F. Lalatta, and O. Zuffardi. "Trisomy 10qter confirmed by in situ hybridisation." Journal of Medical Genetics 30, no. 7 (1993): 601–3. http://dx.doi.org/10.1136/jmg.30.7.601.

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15

Seres-Santamaria, A., V. Catala, E. Cuatrecasas, and R. Villanueva. "Fluorescent in-situ hybridisation and Down's syndrome." Lancet 341, no. 8859 (1993): 1544. http://dx.doi.org/10.1016/0140-6736(93)90690-i.

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16

Bryant, Stewart. "Medicare funding for fluorescence in situ hybridisation." Pathology 39, no. 5 (2007): 535. http://dx.doi.org/10.1080/00313020701621369.

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17

Lerner, Boaz. "Bayesian fluorescence in situ hybridisation signal classification." Artificial Intelligence in Medicine 30, no. 3 (2004): 301–16. http://dx.doi.org/10.1016/j.artmed.2003.11.005.

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18

Hulten, M. A., C. P. Gould, A. S. Goldman, and J. J. Waters. "Chromosome in situ suppression hybridisation in clinical cytogenetics." Journal of Medical Genetics 28, no. 9 (1991): 577–82. http://dx.doi.org/10.1136/jmg.28.9.577.

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19

Campbell, Lynda J. "Fluorescence in situ hybridisation in acute myeloid leukaemia." Pathology 42 (2010): S37. http://dx.doi.org/10.1097/01268031-201042001-00072.

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20

Fleming, K. A. "In-situ hybridisation—a role in clinical pathology." Journal of Pathology 153, no. 3 (1987): 201–2. http://dx.doi.org/10.1002/path.1711530303.

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21

Wolf, Mirela. "Biofilm biodiversity presented by fluorescent in situ hybridisation." E3S Web of Conferences 17 (2017): 00098. http://dx.doi.org/10.1051/e3sconf/20171700098.

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22

Ward, Samantha J., Katherine Karakoula, Kim P. Phipps, et al. "Cytogenetic analysis of paediatric astrocytoma using comparative genomic hybridisation and fluorescence in-situ hybridisation." Journal of Neuro-Oncology 98, no. 3 (2010): 305–18. http://dx.doi.org/10.1007/s11060-009-0081-4.

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23

Bagnoli, Francesca, Susanna Danti, Valentina Magherini, Radiana Cozza, Anna M. Innocenti, and Milvia L. Racchi. "Molecular cloning, characterisation and expression of two catalase genes from peach." Functional Plant Biology 31, no. 4 (2004): 349. http://dx.doi.org/10.1071/fp03203.

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Two cDNA clones encoding catalase (Cat1 and Cat2) from peach [Prunus persica (L.) Batsch] were identified, that show homologies to other plant catalases. The nucleotide sequences of the two coding regions showed 88% identity to each other. The amino acid sequences predicted from the two full-length clones showed the highest homology to a catalase from cotton and Nicotiana plumbaginifolia L. and included C-terminal tri-peptides typical of those used to target proteins to peroxisomes. Southern hybridisation analysis suggested the existence of two catalase genes in peach. The expression of Cat1 a

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24

Tholouli, Eleni, Dolores Di Vizio, Fionnuala O’Connell, et al. "Quantum Dot Based Duplex In Situ Hybridisation for Gene Expression Profiling." Blood 106, no. 11 (2005): 3265. http://dx.doi.org/10.1182/blood.v106.11.3265.3265.

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Abstract Quantum dots (QDs) are fluorescent semiconductor nanocrystals (2–10-nm core diameter) possessing the unique properties of extremely high fluorescence efficiency, lack of photobleaching and long fluorescence lifetime, making them an ideal tool for bioimaging. We have developed a novel technique for in situ hybridisation (ISH) using biotinylated oligonucleotides conjugated to streptavidin coated QD, and used them in this study to label bone marrow trephine samples. 50-mer long oligonucleotide probes were conjugated to QDs prior to ISH and conjugation efficiency was demonstrated by gel e

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25

Goldman, A. S., and M. A. Hulten. "Chromosome in situ suppression hybridisation in human male meiosis." Journal of Medical Genetics 29, no. 2 (1992): 98–102. http://dx.doi.org/10.1136/jmg.29.2.98.

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26

Navikas, V., J. Link, W. Palasik, et al. "Cytokines in myasthenia gravis: an in situ hybridisation study." Neuromuscular Disorders 4, no. 5-6 (1994): S42. http://dx.doi.org/10.1016/0960-8966(94)90175-9.

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27

Schedle, A., M. Willheim, A. Zeitelberger, et al. "Nucleolar morphology and rDNA in situ hybridisation in monocytes." Cell & Tissue Research 269, no. 3 (1992): 473–80. http://dx.doi.org/10.1007/bf00353902.

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28

Ossama, Heba Mohamed, Soha Kholeif, and Ghada Mohamed Elhady. "The Use of Fluorescence In situ Hybridisation in the Diagnosis of Hidden Mosaicism in Egyptian Patients with Turner Syndrome." Journal of Human Reproductive Sciences 16, no. 4 (2023): 286–98. http://dx.doi.org/10.4103/jhrs.jhrs_128_23.

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Abstract Background: Turner syndrome (TS) is the most common chromosomal abnormality in females. The diagnosis of TS is based on karyotyping of 30 blood lymphocytes. This technique does not rule out tissue mosaicism or low-grade mosaicism in the blood. Because of the associated risk of gonadoblastoma, mosaicism is especially important in case this involves a Y chromosome. Aims: This study was set to determine the value of additional genetic studies such as fluorescent in situ hybridisation and the inclusion of buccal cells in search for mosaicism in TS patients. Settings and Design: This cross

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29

Zhao, J. T., M. Frommer, J. A. Sved, and A. Zacharopoulou. "Mitotic and polytene chromosome analyses in the Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae)." Genome 41, no. 4 (1998): 510–26. http://dx.doi.org/10.1139/g98-053.

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The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capitata has been determined by comparing chromosome banding pattern and in situ hybridisation of cloned genes to polytene chromosomes. Although the evidence indicates that a number of chromosomal inversions have occurred since the separation of the two species, synteny of the chromoso

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30

Nicholson, J. "Imbalances of chromosome 17 in medulloblastomas determined by comparative genomic hybridisation and fluorescence in situ hybridisation." Molecular Pathology 53, no. 6 (2000): 313–19. http://dx.doi.org/10.1136/mp.53.6.313.

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31

Fuster, C., L. Miguez, R. Miro, M. A. Rigola, A. Perez, and J. Egozcue. "Familial complex chromosome rearrangement ascertained by in situ hybridisation." Journal of Medical Genetics 34, no. 2 (1997): 164–66. http://dx.doi.org/10.1136/jmg.34.2.164.

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32

Gosden, J. R. "In Situ Hybridisation: Application to Developmental Biology and Medicine." Journal of Medical Genetics 28, no. 6 (1991): 432. http://dx.doi.org/10.1136/jmg.28.6.432.

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33

Amann, Rudolf, Bernhard M. Fuchs, and Sebastian Behrens. "The identification of microorganisms by fluorescence in situ hybridisation." Current Opinion in Biotechnology 12, no. 3 (2001): 231–36. http://dx.doi.org/10.1016/s0958-1669(00)00204-4.

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34

Hopman, A. H. N., Sandra Claessen, and Ernst J. M. Speel. "Multi-colour brightfield in situ hybridisation on tissue sections." Histochemistry and Cell Biology 108, no. 4-5 (1997): 291–98. http://dx.doi.org/10.1007/s004180050168.

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35

Lee, Andrew H. S., Heather P. Key, Jane A. Bell, Zsolt Hodi, and Ian O. Ellis. "Breast carcinomas with borderline (2+) HER2 immunohistochemistry: percentage of cells with complete membrane staining for HER2 and the frequency of HER2 amplification: Table 1." Journal of Clinical Pathology 64, no. 6 (2011): 490–92. http://dx.doi.org/10.1136/jcp.2011.089177.

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AimHER2 status is vital for selecting breast cancer patients for trastuzumab treatment. One recommended approach is to assess immunohistochemical staining and then perform in situ hybridisation on those tumours with a borderline (2+) immunohistochemical result. This audit aimed to assess the value of the percentage of immunohistochemical staining in 2+ tumours in selecting tumours for in-situ hybridisation.MethodsHER2 immunohistochemistry and in situ hybridisation was performed according to UK guidelines. The percentage of complete membrane staining of invasive carcinoma cells for HER2 was rec

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36

Kuipers, Anja GJ, Pat JS Heslop-Harrison, and Evert Jacobsen. "Characterisation and physical localisation of Ty1-copia-like retrotransposons in four Alstroemeria species." Genome 41, no. 3 (1998): 357–67. http://dx.doi.org/10.1139/g98-048.

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The genus Alstroemeria contains species with large genomes (2C = 36.5-78.9 pg (17 600 - 38 000 Mb) in those species with 2n = 2x = 16). We investigated the diversity and genomic and chromosomal organisation of Ty1-copia-like retrotransposons in four Alstroemeria species. Analysis of 33 PCR-amplified sequences corresponding to a conserved domain of the Ty1-copia reverse transcriptase (rt) gene showed high heterogeneity among predicted amino acid sequences; no two sequences were identical, but most fell into one of five subgroups. Levels of inter- and intra-specific heterogeneity of sequences we

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37

Troncone, G., S. M. Anderson, C. S. Herrington, et al. "Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation." Journal of Clinical Pathology 45, no. 10 (1992): 866–70. http://dx.doi.org/10.1136/jcp.45.10.866.

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38

Kokubugata, G., K. Kondo, G. W. Wilson, L. M. Randall, A. van der Schans, and D. K. Morris. "Comparison of karyotype and rDNA-distribution in somatic chromosomes of Bowenia species (Stangeriaceae, Cycadales)." Australian Systematic Botany 13, no. 1 (2000): 15. http://dx.doi.org/10.1071/sb98028.

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Somatic chromosomes at mitotic metaphase of Bowenia serrulata, B. spectabilis and B. sp. ‘Tinaroo’ is investigated by the standard aceto-orcein staining method and the fluorescent in situ hybridisation method (FISH) with ribosomal DNA (rDNA) probe. Bowenia serrulata, B. spectabilis and B. sp. ‘Tinaroo’ each have a chromosome number of 2n = 18. The karyotype of B. serrulata exhibits 10 median-centromeric chromosomes, while B. spectabilis and B. sp. ‘Tinaroo’ exhibit eight median-centromeric chromosomes. By using FISH, B. serrulata, B. spectabilis and B. sp. ‘Tinaroo’ show a hybridisation signal

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39

MCFADDEN, G. "In situ hybridisation in plants: From macroscopic to ultrastructural resolution." Cell Biology International Reports 13, no. 1 (1989): 3–21. http://dx.doi.org/10.1016/s0309-1651(89)80004-9.

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40

Dodd, Susan, Tahir Naeem Khan, and Raja Sinniah. "An in situ Cytomegalovirus DNA Hybridisation Study in IgA Nephritis." Nephron 59, no. 3 (1991): 527. http://dx.doi.org/10.1159/000186635.

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41

Wilkens, Ludwig, Heidrun Gerr, Dorothea Gadzicki, Hans Kreipe, and Brigitte Schlegelberger. "Standardised fluorescence in situ hybridisation in cytological and histological specimens." Virchows Archiv 447, no. 3 (2005): 586–92. http://dx.doi.org/10.1007/s00428-005-1211-9.

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42

Bilous, Michael, Adrienne Morey†, Jane Armes‡, Margaret Cummings, and Glenn Francis. "Chromogenic in situ hybridisation testing for HER2 gene amplification in breast cancer produces highly reproducible results concordant with fluorescence in situ hybridisation and immunohistochemistry." Pathology 38, no. 2 (2006): 120–24. http://dx.doi.org/10.1080/00313020600561518.

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43

Brenk, Christian H., Eva-Christina Prott, Detlef Trost, et al. "Towards mapping phenotypical traits in 18p− syndrome by array-based comparative genomic hybridisation and fluorescent in situ hybridisation." European Journal of Human Genetics 15, no. 1 (2006): 35–44. http://dx.doi.org/10.1038/sj.ejhg.5201718.

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44

Stonati, L., M. Durante, G. Gensabella, et al. "Calibration Curves for Biological Dosimetry by Fluorescence In situ Hybridisation." Radiation Protection Dosimetry 94, no. 4 (2001): 335–45. http://dx.doi.org/10.1093/oxfordjournals.rpd.a006508.

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45

Lory, S., C. von Tscharner, E. Marti, G. Bestetti, S. Grimm, and A. Waldvogel. "In situ hybridisation of equine sarcoids with bovine papilloma virus." Veterinary Record 132, no. 6 (1993): 132–33. http://dx.doi.org/10.1136/vr.132.6.132.

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46

Ramage, Gordon, Sheila Patrick, and Simon Houston. "Combined fluorescent in situ hybridisation and immunolabelling of Bacteroides fragilis." Journal of Immunological Methods 212, no. 2 (1998): 139–47. http://dx.doi.org/10.1016/s0022-1759(98)00005-2.

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47

Warford, Anthony. "In situ hybridisation: Technologies and their application to understanding disease." Progress in Histochemistry and Cytochemistry 50, no. 4 (2016): 37–48. http://dx.doi.org/10.1016/j.proghi.2015.12.001.

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48

Conte, R. A., S. Luke, and R. S. Verma. "Enumeration of semen leucocytes by fluorescence in situ hybridisation technique." Molecular Pathology 48, no. 6 (1995): M319—M321. http://dx.doi.org/10.1136/mp.48.6.m319.

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49

Osborne, Peter, and Peter K. Dearden. "Non-radioactive in-situ hybridisation to honeybee embryos and ovaries." Apidologie 36, no. 1 (2005): 113–18. http://dx.doi.org/10.1051/apido:2004075.

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50

Chatzimeletiou, Katerina, George Makrydimas, Alexandros Sotiriadis, Evagelos Paraskevaidis, and Kypros H. Nicolaides. "Aneuploidy screening in coelomic samples using fluorescencein situ hybridisation (FISH)." Prenatal Diagnosis 25, no. 10 (2005): 919–26. http://dx.doi.org/10.1002/pd.1227.

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