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Relevant bibliographies by topics / In vitro cultured

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Academic literature on the topic 'In vitro cultured'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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Journal articles on the topic "In vitro cultured"

1

Nishii, R., K. Imai, H. Koyama, and O. Dochi. "166 EFFECT OF INDIVIDUAL CULTURE SYSTEM ON IN VITRO DEVELOPMENT OF IN VITRO-MATURED - IN VITRO-FERTILIZED BOVINE EMBRYOS." Reproduction, Fertility and Development 24, no. 1 (2012): 195. http://dx.doi.org/10.1071/rdv24n1ab166.

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An individual in vitro culture system for bovine embryo needs to be developed for the study of embryo developmental competence. The objective of this study was to examine the effect of individual culture systems on the development of in vitro-matured–in vitro-fertilized bovine embryos. Two individual culture systems were compared. Cumulus–oocyte complexes (COC) were collected by aspiration of ovarian follicles (diameter, 2 to 5 mm) obtained from a local abattoir. The COC were matured in TCM-199 supplemented with 5% calf serum and 0.02 AU mL–1 of FSH. Groups of 20 COC were incubated in 100-μL d

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du Preez, Francois, Antoinette Paula Malan, and Pia Addison. "Potential of in vivo- and in vitro-cultured entomopathogenic nematodes to infect Lobesia vanillana (Lepidoptera: Tortricidae) under laboratory conditions." PLOS ONE 16, no. 8 (2021): e0242645. http://dx.doi.org/10.1371/journal.pone.0242645.

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Entomopathogenic nematodes (EPNs) have been successfully applied as biological control agents against above ground and soil stages of insect pests. However, for commercial application, it is crucial to mass culture these nematodes using in vitro liquid culture technology, as it is not attainable when using susceptible insects as hosts. Lobesia vanillana (Lepidoptera: Tortricidae) is regarded a sporadic pest of wine grapes in South Africa. The in vivo- and in vitro-cultured South African EPNs, Steinernema yirgalemense and Steinernema jeffreyense (Rhabditida: Steinernematidae), were evaluated ag

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Jarrahy, Reza, Weibiao Huang, George H. Rudkin, et al. "Osteogenic differentiation is inhibited and angiogenic expression is enhanced in MC3T3-E1 cells cultured on three-dimensional scaffolds." American Journal of Physiology-Cell Physiology 289, no. 2 (2005): C408—C414. http://dx.doi.org/10.1152/ajpcell.00196.2004.

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Osteogenic differentiation of osteoprogenitor cells in three-dimensional (3D) in vitro culture remains poorly understood. Using quantitative real-time RT-PCR techniques, we examined mRNA expression of alkaline phosphatase, osteocalcin, and vascular endothelial growth factor (VEGF) in murine preosteoblastic MC3T3-E1 cells cultured for 48 h and 14 days on conventional two-dimensional (2D) poly(l-lactide-co-glycolide) (PLGA) films and 3D PLGA scaffolds. Differences in VEGF secretion and function between 2D and 3D culture systems were examined using Western blots and an in vitro Matrigel-based ang

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Silva, J. R. V., T. Tharasanit, M. A. M. Taverne, et al. "The activin-follistatin system and in vitro early follicle development in goats." Journal of Endocrinology 189, no. 1 (2006): 113–25. http://dx.doi.org/10.1677/joe.1.06487.

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The aim of the present study was to investigate the effects of activin-A and follistatin on in vitro primordial and primary follicle development in goats. To study primordial follicle development (experiment 1), pieces of ovarian cortex were cultured in vitro for 5 days in minimal essential medium (MEM) supplemented with activin-A (0, 10 or 100 ng/ml), follistatin (0, 10 or 100 ng/ml) or combinations of the two. After culture, the numbers of primordial follicles and more advanced follicle stages were calculated and compared with those in non-cultured tissue. Protein and mRNA expression of acti

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Kobayashi, Tsunehiro, Hossein Arefanian, George Harb, et al. "Prolonged Survival of Microencapsulated Neonatal Porcine Islet Xenografts in Immune-Competent Mice without Antirejection Therapy." Cell Transplantation 17, no. 10-11 (2008): 1243–56. http://dx.doi.org/10.3727/096368908787236602.

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Several studies have demonstrated that in vitro culture of islets prolonged islet graft survival in immune-competent mice without administration of antirejection drugs. However, we recently showed that in vitro cultured microencapsulated neonatal porcine islets (NPI) were rejected in immune-competent mice not receiving antirejection therapy. The aim of this study was to determine whether culture of microencapsulated NPI in vivo could promote long-term survival of microencapsulated NPI in immune-competent mice without administration of antirejection drugs. Microencapsulated NPI that were cultur

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Wilson, Timothy, Reeta Viitala, Mervi Puska, Mika Jokinen, and Risto Penttinen. "Macrophage Induced Effect of Particulate Silica on Rat Mesenchymal Stem Cells In Vitro." Key Engineering Materials 396-398 (October 2008): 123–26. http://dx.doi.org/10.4028/www.scientific.net/kem.396-398.123.

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The role of silica and macrophages in fibrosis is well documented, but in bone formation it is relatively unknown despite decades of research with bioactive glasses. In this study macrophages were isolated from rat peritoneal and then cultured for five days in the presence of two types of silica microparticles with different solubilities. After the fifth day the culture medium was collected, purified and used as an additive in bone marrow derived rat stem cell cultures. The stem cells were cultured for five days in α-mem containing only 0,5% of FCS, enabling cell survival but disrupting their

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Nas, Mehmet Nuri, Nedim Mutlu, and Paul E. Read. "Random Amplified Polymorphic DNA (RAPD) Analysis of Long-term Cultured Hybrid Hazelnut." HortScience 39, no. 5 (2004): 1079–82. http://dx.doi.org/10.21273/hortsci.39.5.1079.

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RAPD and phenotypic analysis were conducted to assess clonal stability of hazelnuts generated from axillary buds cultured in vitro for long-term. The nuts produced on in vitro-propagated plants were indistinguishable from those of donor plants. With the exception of rare horizontal (plagiotropic) growth, all in vitro-propagated plants exhibited phenotypes similar to those of donor plants. RAPD analysis did not reveal any somaclonal variation between donor plants from which in vitro cultures were initiated and micropropagated plants (6-year cultures), and no somaclonal variation was detected am

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Racusen, L. C., B. A. Fivush, H. Andersson, and W. A. Gahl. "Culture of renal tubular cells from the urine of patients with nephropathic cystinosis." Journal of the American Society of Nephrology 1, no. 8 (1991): 1028–33. http://dx.doi.org/10.1681/asn.v181028.

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Nephropathic cystinosis represents a prototype for lysosomal storage diseases and is the most common cause of renal tubular Fanconi's syndrome. Mechanisms of the tubular transport defects in this disease have not been defined, however, in part because the cells readily cultured from affected patients, leukocytes and fibroblasts, do not express epithelial transport functions. Except for a single autopsy report, renal tubular cells from these patients have not been studied in vitro. In these studies, noninvasive harvesting and culture of renal tubular cells from the urine of patients with cystin

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Al-Juboory, Karim H., and Jabar H. Al-Niami. "Adventitious Shoot and Plantlet Formation from Cultured Apple Leaf Explants." HortScience 31, no. 4 (1996): 629f—629. http://dx.doi.org/10.21273/hortsci.31.4.629f.

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Leaves of wild apple (Malus domestica Borkh) were excised from in vitro grown shoots transversely cut into halves and plated onto petri dishes containing regeneration media. Cultures were kept in the dark for three weeks before adventitious shoots were observed. Callus from leaf explants produced adventitious shoots after 3 months of in vitro culture. Callus were cultured on Nitsch and Nitsch medium supplemented with a range of BA (0.0–2.0 μm) and NAA (0.0–10 μm). BA at 10 μm combined with NAA (0.5 μm) proved most effective for stimulating shoot proliferation of cultured apple. Plantlets from

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Romberger, D. J., P. Pladsen, L. Claassen, M. Yoshida, J. D. Beckmann, and S. I. Rennard. "Insulin modulation of bronchial epithelial cell fibronectin in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 268, no. 2 (1995): L230—L238. http://dx.doi.org/10.1152/ajplung.1995.268.2.l230.

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Fibronectin (Fn) is involved in the migration of epithelial cells in re-epithelialization of wounds. Epithelial cell-derived Fn is particularly potent as a chemotactic factor for bronchial epithelial cells (BECs) in vitro. Thus modulation of airway epithelial cell Fn may be a key aspect of airway repair. Insulin is both an important growth factor and known chemotactic factor for cultured BECs. We postulated that insulin may modulate Fn production of cultured BECs. We examined this hypothesis utilizing bovine BECs in culture with serum-free media with and without insulin. BECs grown in media wi

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Dissertations / Theses on the topic "In vitro cultured"

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Cookson, Mark R. "Studies of activation and toxicity in cultured astrocytes." Thesis, University of Salford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308094.

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Lawrence, J. N. "Cryopreservation and toxicity studies with cultured rat and human hepatocytes." Thesis, University of Surrey, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233123.

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Gough, Julie. "Apoptosis of human osteoblasts cultured on polymeric biomaterials in vitro." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298960.

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Gibson, Bethany Gale. "Improving the development of bovine in vitro produced embryos cultured individually." Thesis, Virginia Tech, 2014. http://hdl.handle.net/10919/49694.

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Previous research in bovine embryology has found that embryos cultured individually have limited ability to develop compared to their counterparts cultured in a group of other embryos. This investigation aimed to find if any of three different interventions over two experiments would increase development of individually cultured embryos to that of group cultured embryos. In the first experiment both the addition of serum/serum replacer and a co-culture with bovine granulosa cells were applied to individually cultured embryos in a 3x2 design. None of the interventions was found to be signifi

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Pijut, Paula M. "Effects of culture filtrates of Ceratocystis ulmi on growth and ultrastructure of in vitro cultured Ulmus Americana /." The Ohio State University, 1988. http://rave.ohiolink.edu/etdc/view?acc_num=osu148759742413622.

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Kawarsky, Sheldon Jerald. "Effects of elevated temperature on bovine oocytes and embryos cultured in vitro." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0001/NQ40376.pdf.

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Bedi, Seema. "Environmental stress and the response of pinus caribaea Morelet cultured in vitro." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335043.

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Turgut, Emine. "Characterisation and detection of Renibacterium salmoninarum cultured in vivo and in vitro." Thesis, University of Stirling, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250258.

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Van, Tonder Jacob John. "Development of an in vitro mechanistic toxicity screening model using cultured hepatocytes." Thesis, University of Pretoria, 2011. http://hdl.handle.net/2263/24162.

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In vitro testing includes both cell-based and cell-free systems that can be used to detect toxicity induced by xenobiotics. In vitro methods are especially useful in rapidly gathering intelligence regarding the toxicity of compounds for which none is available such as new chemical entities developed in the pharmaceutical industry. In addition to this, in vitro investigations are invaluable in providing information concerning mechanisms of toxicity of xenobiotics. This type of toxicity testing has gained popularity among the research and development community because of a number of advantages s

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Koeman, Jennifer. "Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33417.

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In vitro production of embryos from oocytes recovered by laparoscopic oocyte pick-up (LOPU) offers great potential for the propagation of genetically valuable animals. In turn, the application of these techniques to prepubertal animals presents added benefits in that prepubertal animals may supply a greater number of oocytes than adult animals. The aim of the present study was to evaluate the developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media. The follicular response and recovery of oocytes via LOPU from hormonally stimulated prepubertal and adult goa

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Books on the topic "In vitro cultured"

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Rhône-Poulenc Rorer Round Table Conference (6th 1990 Les Pensières, France). In vitro methods in toxicology. Academic, 1992.

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M, Pierik R. L. In vitro culture of higher plants. 4th ed. M. Nijhoff, 1997.

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M, Pierik R. L. In vitro culture of higher plants. M. Nijhoff, 1987.

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Pierik, R. L. M. In vitro culture of higher plants. 3rd ed. Kluwer Academic Publishers, 1997.

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M, Pierik R. L. In vitro culture of higher plants. Kluwer Academic Publishers, 1997.

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Alan, Trounson, and Wood Carl, eds. Atlas of fine structure of human sperm penetration, eggs, and embryos cultured in vitro. Praeger, 1985.

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Biology of normal proliferating cells in vitro: Relevance for in vivo aging. Karger, 1988.

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S, Ambesi-Impiombato F., and Perrild H, eds. FRTL-5 today: Proceedings of the First International Workshop on Characterization and Standardization of an In Vitro Thyroid Cell System, Udine, Italy, 26-28 October 1988. Excerpta Medica, 1989.

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Bonga, J. M., and P. von Aderkas. In Vitro Culture of Trees. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-015-8058-8.

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Declerck, Stéphane, J. André Fortin, and Désiré-Georges Strullu, eds. In Vitro Culture of Mycorrhizas. Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/b138925.

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Book chapters on the topic "In vitro cultured"

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Cookson, Mark R., R. McClean, and Vietor W. Pentreatk. "Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity." In In Vitro Toxicity Testing Protocols. Humana Press, 1995. http://dx.doi.org/10.1385/0-89603-282-5:17.

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Jona, R., A. Fronda, A. Cattro, and A. Gallo. "Ethylene Synthesis by Fruit Plants Cultured in Vitro." In Cellular and Molecular Aspects of the Plant Hormone Ethylene. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-017-1003-9_86.

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Patérour, M. J., A. Renier, J. Bignon, and M. C. Jaurand. "Induction of Transformation in Cultured Rat Pleural Mesothelial Cells by Chrysotile Fibres." In In Vitro Effects of Mineral Dusts. Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-70630-1_25.

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Jaurand, M. C. "Comparative Responses of Cultured Cells to Asbestos Fibres in Relation to Carcinogenicity." In In Vitro Effects of Mineral Dusts. Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-70630-1_27.

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Mori, Nozomu. "In Vitro Aging Revisited: The Longevity of Cultured Neurons." In Aging Mechanisms. Springer Japan, 2015. http://dx.doi.org/10.1007/978-4-431-55763-0_5.

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Prentoe, Jannick, and Jens Bukh. "In Vitro Neutralization Assay Using Cultured Hepatitis C Virus." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8976-8_29.

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Medrano, L., L. Kheuang, M. J. Patérour, J. Bignon, and M. C. Jaurand. "Chromosomal Changes in Cultured Rat Mesothelial Cells Treated with Benzo-3-4- Pyrene and/or Chrysotile Asbestos." In In Vitro Effects of Mineral Dusts. Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-70630-1_66.

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Guerra, Liliana, Elena Dellambra, Patrizia Paterna, and Somesh Gupta. "Safety Concerns in Transplantation of In-Vitro -Cultured Cellular Grafts." In Vitiligo. John Wiley & Sons, Ltd, 2018. http://dx.doi.org/10.1002/9781118937303.ch41.

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Ochatt, Sergio J. "Immature Seeds and Embryos of Medicago truncatula Cultured In Vitro." In Methods in Molecular Biology. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-61737-988-8_4.

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Santamaria, Jorge M., and William J. Davies. "Control of water loss by delphinium plants cultured in vitro." In Physiology, Growth and Development of Plants in Culture. Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0790-7_16.

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Conference papers on the topic "In vitro cultured"

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LI, Xiaojuan, Xiaorui XIE, Jin REN, Liling REN, and Jian CAO. "Effects of Propranolol on Osteoclasts Cultured in Vitro." In International Conference on Biological Engineering and Pharmacy 2016 (BEP 2016). Atlantis Press, 2017. http://dx.doi.org/10.2991/bep-16.2017.6.

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Segawa, Naoki, and Yasuhiko Sugii. "Velocity Measurement of In Vitro Blood Flow in Microchip Cultured Endothelial Cells." In ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer. ASMEDC, 2008. http://dx.doi.org/10.1115/mnht2008-52250.

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In order to investigate vascular diseases such as cause of atherosclerosis and myocardial infarction, relationships of endothelial cells (ECs) covered with surface blood vessels and blood flow stimulation have been experimentally studied. In the study, a blood vessel model for in vitro experiment made from polydimethylsiloxane (PDMS) microchip with a straight microchannel with 400 μm width and 100 μm depth was developed. By optimizing cells cultured condition such as the liquid introduction method and the surface coating for enhancement of cell attachment on the microchannel wall, cell culture

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Lee, Cynthia R., Mauro Alini, and James C. Iatridis. "Organ Culture System for Mechanobiology Studies of the Intervertebral Disc." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-61248.

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The development of in vitro models is critical for furthering understanding of the intervertebral disc and the development of disc regeneration/tissue engineering. An in vitro culture system targeted towards mechano-biology studies of the intervertebral disc (IVD) was built and validated using bovine coccygeal discs. Discs were maintained in culture for up to one week with and without vertebral endplates. Water content and glycosaminoglycan content were found to be stable and cells were metabolically active when cultured under a 5kg static load.

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von Reyn, Jessica A., Mary E. Cloutier, Jason H. T. Bates, and Gilman B. Allen. "Acid Aspiration Injury Amplifies In Vitro Endotoxin Signaling In Cultured Macrophages." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a6477.

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Wang, Lin, Feijuan Huang, Zhengzhi Wu, and Rui Ma. "Biocompatibility of nano-hydroxyapatite/polyetheretherketone composite materials with osteoblasts cultured in vitro." In ADVANCES IN ENERGY SCIENCE AND ENVIRONMENT ENGINEERING: Proceedings of the 2017 International Workshop on Advances in Energy Science and Environment Engineering (AESEE 2017). Author(s), 2017. http://dx.doi.org/10.1063/1.4979736.

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Ma, Liang, Jeremy Barker, Changchun Zhou, Biaoyang Lin, and Wei Li. "A Perfused Two-Chamber System for Anticancer Drug Screening." In ASME 2010 International Manufacturing Science and Engineering Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/msec2010-34326.

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A cell culture microfluidic device has been developed to test the cytotoxicity of anticancer drugs while reproducing multi-organ interactions in vitro. Cells were cultured in separate chambers representing the liver and tumor. The two chambers were connected through a channel to mimick the blood flow. Glioblastoma (GBM) cancer cells (M059K) and hepatoma cells (HepG2) were cultured in the tumor and the liver chambers, respectively. The cytotoxic effect of cancer treatment drug Temolozomide (TMZ) was tested using this two chamber system. The experimental results showed that with the liver cells,

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Tung, Yen-Ting, and Gou-Jen Wang. "In Vitro Development of Microvessels Using a Scaffold of Cylindrical PLGA." In ASME 2016 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/detc2016-59550.

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The microvascular network is a simple but critical system that is responsible for various important biological mechanisms in the bodies of all animals. The ability to generate a functional microvessel in vitro not only makes it possible to engineer vital tissue of considerable size but also serves as a platform for biomedical studies. In this study, we propose a simple method for fabricating customized cylinder micro-scaffolds for the in vitro development of microvascular networks. By integrating micro-electro-mechanical systems techniques with thermal reflow, we design and fabricate a micro-s

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Whitton, Andrew, David J. Flint, and Richard A. Black. "Development of a Compliant Electrospun Polyurethane Vascular Graft." In ASME 2010 5th Frontiers in Biomedical Devices Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/biomed2010-32070.

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Synthetic vascular grafts are an integral tool in vascular surgery. However, the consistent failure of small diameter grafts is one of the main limitations of these devices. For this reason electrospun polyurethane has been investigated for its suitability as a vascular substitute material in this present study. Aligned and random mesh electrospun polyurethane materials were produced and analysed in vitro by investigating the effect of using both materials as a substrate for the culture of human aortic smooth muscle cells. Immunofluorescence analysis showed that cells cultured on electrospun p

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Khotskova, L. V., G. Ya Stepanyuk, and T. P. Astafurova. "INFLUENCE OF GROWTH CONDITIONS ON MORPHOGENESIS OF CYMBIDIUM HYBRIDUM SEEDLINGS CULTURED IN VITRO." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-1414-1417.

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Boukallel, M., M. Girot, and S. Regnier. "Elastic Properties Exploration of In Vitro Cultured Microscopic Cells based on Haptic Sensing." In 2006 IEEE Conference on Virtual Environments, Human-Computer Interfaces and Measurement Systems. IEEE, 2006. http://dx.doi.org/10.1109/vecims.2006.250785.

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Reports on the topic "In vitro cultured"

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Grover, Paramjit, M. F. Rahman, and M. Mahboob. Bio-Physicochemical Interactions of Engineered Nanomaterials in In Vitro Cell Culture Model. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada567065.

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Hawkins, Brian T., and Sonia Grego. A Better, Faster Road From Biological Data to Human Health: A Systems Biology Approach for Engineered Cell Cultures. RTI Press, 2017. http://dx.doi.org/10.3768/rtipress.2017.rb.0015.1706.

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Traditionally, the interactions of drugs and toxicants with human tissue have been investigated in a reductionist way—for example, by focusing on specific molecular targets and using single-cell-type cultures before testing compounds in whole organisms. More recently, “systems biology” approaches attempt to enhance the predictive value of in vitro biological data by adopting a comprehensive description of biological systems and using computational tools that are sophisticated enough to handle the complexity of these systems. However, the utility of computational models resulting from these eff

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Guzman, Juantia J., Clark L. Gross, William J. Smith, and Susan A. Kelly. In Vitro Cytotoxicity Assays of Human Epidermal Keratinocytes in Culture Exposed to Sulfur Mustard. Defense Technical Information Center, 2000. http://dx.doi.org/10.21236/ada390628.

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Zayova, Ely, Elisaveta Kitova, and Maria Geneva. Optimized Cultural Conditions for Rapid in Vitro Propagation and Conservation of Mentha piperita L. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, 2021. http://dx.doi.org/10.7546/crabs.2021.06.18.

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Nasircilar, Ayse Gul. In vitro Clonal Propagation of Endemic Allium junceum Subs. tridentatum via Immature and Mature Embryo Culture Method. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, 2021. http://dx.doi.org/10.7546/crabs.2021.08.18.

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Ulukapi, Kamile, and Ayse Gul Nasircilar. Mutation Breeding Protocol for Development of Drought-tolerant Genotypes in Phaseolus vulgaris L. Using in-vitro Embryo Culture Techniques. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, 2020. http://dx.doi.org/10.7546/crabs.2020.11.10.

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