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Journal articles on the topic 'In vitro cultured'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Nishii, R., K. Imai, H. Koyama, and O. Dochi. "166 EFFECT OF INDIVIDUAL CULTURE SYSTEM ON IN VITRO DEVELOPMENT OF IN VITRO-MATURED - IN VITRO-FERTILIZED BOVINE EMBRYOS." Reproduction, Fertility and Development 24, no. 1 (2012): 195. http://dx.doi.org/10.1071/rdv24n1ab166.

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An individual in vitro culture system for bovine embryo needs to be developed for the study of embryo developmental competence. The objective of this study was to examine the effect of individual culture systems on the development of in vitro-matured–in vitro-fertilized bovine embryos. Two individual culture systems were compared. Cumulus–oocyte complexes (COC) were collected by aspiration of ovarian follicles (diameter, 2 to 5 mm) obtained from a local abattoir. The COC were matured in TCM-199 supplemented with 5% calf serum and 0.02 AU mL–1 of FSH. Groups of 20 COC were incubated in 100-μL d

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2

du Preez, Francois, Antoinette Paula Malan, and Pia Addison. "Potential of in vivo- and in vitro-cultured entomopathogenic nematodes to infect Lobesia vanillana (Lepidoptera: Tortricidae) under laboratory conditions." PLOS ONE 16, no. 8 (2021): e0242645. http://dx.doi.org/10.1371/journal.pone.0242645.

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Entomopathogenic nematodes (EPNs) have been successfully applied as biological control agents against above ground and soil stages of insect pests. However, for commercial application, it is crucial to mass culture these nematodes using in vitro liquid culture technology, as it is not attainable when using susceptible insects as hosts. Lobesia vanillana (Lepidoptera: Tortricidae) is regarded a sporadic pest of wine grapes in South Africa. The in vivo- and in vitro-cultured South African EPNs, Steinernema yirgalemense and Steinernema jeffreyense (Rhabditida: Steinernematidae), were evaluated ag

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3

Jarrahy, Reza, Weibiao Huang, George H. Rudkin, et al. "Osteogenic differentiation is inhibited and angiogenic expression is enhanced in MC3T3-E1 cells cultured on three-dimensional scaffolds." American Journal of Physiology-Cell Physiology 289, no. 2 (2005): C408—C414. http://dx.doi.org/10.1152/ajpcell.00196.2004.

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Osteogenic differentiation of osteoprogenitor cells in three-dimensional (3D) in vitro culture remains poorly understood. Using quantitative real-time RT-PCR techniques, we examined mRNA expression of alkaline phosphatase, osteocalcin, and vascular endothelial growth factor (VEGF) in murine preosteoblastic MC3T3-E1 cells cultured for 48 h and 14 days on conventional two-dimensional (2D) poly(l-lactide-co-glycolide) (PLGA) films and 3D PLGA scaffolds. Differences in VEGF secretion and function between 2D and 3D culture systems were examined using Western blots and an in vitro Matrigel-based ang

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4

Silva, J. R. V., T. Tharasanit, M. A. M. Taverne, et al. "The activin-follistatin system and in vitro early follicle development in goats." Journal of Endocrinology 189, no. 1 (2006): 113–25. http://dx.doi.org/10.1677/joe.1.06487.

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The aim of the present study was to investigate the effects of activin-A and follistatin on in vitro primordial and primary follicle development in goats. To study primordial follicle development (experiment 1), pieces of ovarian cortex were cultured in vitro for 5 days in minimal essential medium (MEM) supplemented with activin-A (0, 10 or 100 ng/ml), follistatin (0, 10 or 100 ng/ml) or combinations of the two. After culture, the numbers of primordial follicles and more advanced follicle stages were calculated and compared with those in non-cultured tissue. Protein and mRNA expression of acti

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5

Kobayashi, Tsunehiro, Hossein Arefanian, George Harb, et al. "Prolonged Survival of Microencapsulated Neonatal Porcine Islet Xenografts in Immune-Competent Mice without Antirejection Therapy." Cell Transplantation 17, no. 10-11 (2008): 1243–56. http://dx.doi.org/10.3727/096368908787236602.

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Several studies have demonstrated that in vitro culture of islets prolonged islet graft survival in immune-competent mice without administration of antirejection drugs. However, we recently showed that in vitro cultured microencapsulated neonatal porcine islets (NPI) were rejected in immune-competent mice not receiving antirejection therapy. The aim of this study was to determine whether culture of microencapsulated NPI in vivo could promote long-term survival of microencapsulated NPI in immune-competent mice without administration of antirejection drugs. Microencapsulated NPI that were cultur

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Wilson, Timothy, Reeta Viitala, Mervi Puska, Mika Jokinen, and Risto Penttinen. "Macrophage Induced Effect of Particulate Silica on Rat Mesenchymal Stem Cells In Vitro." Key Engineering Materials 396-398 (October 2008): 123–26. http://dx.doi.org/10.4028/www.scientific.net/kem.396-398.123.

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The role of silica and macrophages in fibrosis is well documented, but in bone formation it is relatively unknown despite decades of research with bioactive glasses. In this study macrophages were isolated from rat peritoneal and then cultured for five days in the presence of two types of silica microparticles with different solubilities. After the fifth day the culture medium was collected, purified and used as an additive in bone marrow derived rat stem cell cultures. The stem cells were cultured for five days in α-mem containing only 0,5% of FCS, enabling cell survival but disrupting their

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7

Nas, Mehmet Nuri, Nedim Mutlu, and Paul E. Read. "Random Amplified Polymorphic DNA (RAPD) Analysis of Long-term Cultured Hybrid Hazelnut." HortScience 39, no. 5 (2004): 1079–82. http://dx.doi.org/10.21273/hortsci.39.5.1079.

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RAPD and phenotypic analysis were conducted to assess clonal stability of hazelnuts generated from axillary buds cultured in vitro for long-term. The nuts produced on in vitro-propagated plants were indistinguishable from those of donor plants. With the exception of rare horizontal (plagiotropic) growth, all in vitro-propagated plants exhibited phenotypes similar to those of donor plants. RAPD analysis did not reveal any somaclonal variation between donor plants from which in vitro cultures were initiated and micropropagated plants (6-year cultures), and no somaclonal variation was detected am

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8

Racusen, L. C., B. A. Fivush, H. Andersson, and W. A. Gahl. "Culture of renal tubular cells from the urine of patients with nephropathic cystinosis." Journal of the American Society of Nephrology 1, no. 8 (1991): 1028–33. http://dx.doi.org/10.1681/asn.v181028.

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Nephropathic cystinosis represents a prototype for lysosomal storage diseases and is the most common cause of renal tubular Fanconi's syndrome. Mechanisms of the tubular transport defects in this disease have not been defined, however, in part because the cells readily cultured from affected patients, leukocytes and fibroblasts, do not express epithelial transport functions. Except for a single autopsy report, renal tubular cells from these patients have not been studied in vitro. In these studies, noninvasive harvesting and culture of renal tubular cells from the urine of patients with cystin

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Al-Juboory, Karim H., and Jabar H. Al-Niami. "Adventitious Shoot and Plantlet Formation from Cultured Apple Leaf Explants." HortScience 31, no. 4 (1996): 629f—629. http://dx.doi.org/10.21273/hortsci.31.4.629f.

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Leaves of wild apple (Malus domestica Borkh) were excised from in vitro grown shoots transversely cut into halves and plated onto petri dishes containing regeneration media. Cultures were kept in the dark for three weeks before adventitious shoots were observed. Callus from leaf explants produced adventitious shoots after 3 months of in vitro culture. Callus were cultured on Nitsch and Nitsch medium supplemented with a range of BA (0.0–2.0 μm) and NAA (0.0–10 μm). BA at 10 μm combined with NAA (0.5 μm) proved most effective for stimulating shoot proliferation of cultured apple. Plantlets from

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10

Romberger, D. J., P. Pladsen, L. Claassen, M. Yoshida, J. D. Beckmann, and S. I. Rennard. "Insulin modulation of bronchial epithelial cell fibronectin in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 268, no. 2 (1995): L230—L238. http://dx.doi.org/10.1152/ajplung.1995.268.2.l230.

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Fibronectin (Fn) is involved in the migration of epithelial cells in re-epithelialization of wounds. Epithelial cell-derived Fn is particularly potent as a chemotactic factor for bronchial epithelial cells (BECs) in vitro. Thus modulation of airway epithelial cell Fn may be a key aspect of airway repair. Insulin is both an important growth factor and known chemotactic factor for cultured BECs. We postulated that insulin may modulate Fn production of cultured BECs. We examined this hypothesis utilizing bovine BECs in culture with serum-free media with and without insulin. BECs grown in media wi

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11

Guo, W., K. Kamiya, J. Cheng, and J. Toyama. "Changes in action potentials and ion currents in long-term cultured neonatal rat ventricular cells." American Journal of Physiology-Cell Physiology 271, no. 1 (1996): C93—C102. http://dx.doi.org/10.1152/ajpcell.1996.271.1.c93.

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A primary culture of neonatal ventricular myocytes isolated from day-old rats was established for investigating the changes in action potentials and ion currents over long periods. Cells at days 5 and 15 in culture were studied. These changes in vitro were compared with those in situ derived from the age-matched freshly isolated cells. During primary culture, quiescent cells demonstrated shortening of action potential durations (APD) resembling the developmental changes observed in situ. The beating cultured cells were not associated with APD shortening. Despite constant current amplitudes, th

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12

Alexander, J. Steven, and Thomas M. Crisp. "Gonadotrophic regulation of prolactin mediated progesterone secretion in vitro." Acta Endocrinologica 110, no. 2 (1985): 251–56. http://dx.doi.org/10.1530/acta.0.1100251.

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Abstract. The effects of preincubating rat granulosa cells with FSH, LH, and Prl on subsequent Prl mediated progesterone secretion were investigated. Granulosa cells were isolated from ovarian follicles 50 h after injection of 5 IU PMSG and were then plated on poly-l-lysine coated coverslips in serum supplemented medium. Cells were preincubated for 24 h in the absence of hormones (control) or with the addition of either 0.25, 2.5, 25 ng/ml rat FSH or rat LH, or 1 μg/ml rat Prl. Following the preincubation period, cells were maintained for an additional 6 or 8 days in the presence or absence of

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13

Shannon, J. M., S. D. Jennings, and L. D. Nielsen. "Modulation of alveolar type II cell differentiated function in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 262, no. 4 (1992): L427—L436. http://dx.doi.org/10.1152/ajplung.1992.262.4.l427.

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We have investigated whether the loss of differentiated function observed in adult rat alveolar type II cells cultured on a substratum that promotes cell spreading and flattening represents a reversible phenotypic change. Cells were cultured for 4 and 8 days in association with fetal rat lung fibroblast feeder layers on either attached collagen gels, which promote the loss of differentiated function, or on floating collagen gels, which support differentiation. A fifth group of cultures were maintained as attached gels for 4 days, then detached and cultured as floating gels for the remaining 4

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da Silva, Cleidson Manoel Gomes, Simone Vieira Castro, Luciana Rocha Faustino, et al. "Activin-A promotes the development of goat isolated secondary follicles in vitro." Zygote 23, no. 1 (2013): 41–52. http://dx.doi.org/10.1017/s0967199413000294.

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SummaryThe role of activin-A in follicular development and on the mRNA expression levels of different genes in goat secondary follicles was evaluated. Goat secondary follicles (≥150 μm) were cultured for 18 days under control conditions or with the addition of either 50 or 100 ng/ml activin-A (Experiment 1). The mRNA levels for the genes that code for activin-A, ActR-IA, ActR-IB, ActR-IIA, ActR-IIB, follicle stimulating hormone receptor (FSH-R) and P450 aromatase were measured in each condition (Experiment 2). We observed that after 6 days of culture, the antrum formation rate was higher in cu

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15

Stimart, Dennis P., John C. Mather, and Kenneth R. Schroeder. "Shoot Proliferation and Rooting in Vitro of Pulmonaria." HortScience 33, no. 2 (1998): 339–41. http://dx.doi.org/10.21273/hortsci.33.2.339.

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Expanding shoot tips of Pulmonaria `Roy Davidson' and Pulmonaria saccharata `Margery Fish' were cultured in vitro on a modified Murashige and Skoog medium containing BA to establish proliferating cultures for use in comparing BA concentrations on shoot proliferation and rooting. The optimum level for shoot proliferation was 8.8 μm BA. Greatest rooting was on medium without BA. Genotype and time in culture influenced shoot and root counts. Chemical names used: N6-benzyladenine (BA)

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16

Mrázová, Veronika, Tatiana Betáková, Marcela Kúdelová, et al. "Murine Gammaherpesvirus (MHV-68) Transforms Cultured Cells in vitro." Intervirology 58, no. 2 (2015): 69–72. http://dx.doi.org/10.1159/000370071.

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Human dermal fibroblasts and mouse NIH/3T3 cells acquired the transformed phenotype (‘criss-cross' pattern of growth) after infection with ultraviolet-irradiated murine gammaherpesvirus (MuHV-4 strain 68; MHV-68). These cells with changed phenotype could be serially cultured for 5-6 passages (35-40 days), and then they entered into crisis and most of them died. In a small number of cultures, however, foci of newly transformed cells appeared from which two stable cell lines were derived. After 6-9 cell culture passages of the MHV-68 transformed cell lines, MHV-68 DNA and virus antigen could be

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17

Iida, Jin, Takafumi Yoshikawa, Kazuhide Miyazaki, Noriko Okumura, and Yoshinori Takakura. "Osteogenic Potential of Estriol-Treated Cultured Bone - In Vitro and In Vivo -." Key Engineering Materials 284-286 (April 2005): 651–54. http://dx.doi.org/10.4028/www.scientific.net/kem.284-286.651.

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Osseous tissue can be formed by culturing marrow cells with compounds such as dexamethasone and that a bone matrix cultured in this manner possesses BMP activity. We have reported that artificial bones with a high level of osteogenic potential can be prepared by culturing artificial bone materials with cultured osseous tissue. Here, in an attempt to develop activated cultured bone constructs with even greater osteogenic potential, the effects of the female hormone estriol on osteogenesis were investigated. Bone marrow cells were collected from the femur shafts of 7-week-old male Fischer rats,

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18

Walum, Erik, Elisabeth Hansson, and Alan L. Harvey. "In Vitro Testing of Neurotoxicity." Alternatives to Laboratory Animals 18, no. 1_part_1 (1990): 153–79. http://dx.doi.org/10.1177/026119299001800118.1.

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Many of the toxic compounds that are at large in the environment represent a risk to our neuronal functions. Chemicals may have a direct or indirect effect on the nervous system and they may interfere with general biochemical properties or specific neuronal structures and processes. In this review, a brief presentation of the major neurotoxicological targets is given, together with a discussion of some aspects of the use of different in vitro models for screening purposes and mechanistic studies. It is believed that in vitro methods offer special opportunities for the development of new neurot

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Kancherla, S. Latha, and Prem L. Bhalla. "In Vitro Propagation of Pandoreas." HortScience 36, no. 2 (2001): 348–50. http://dx.doi.org/10.21273/hortsci.36.2.348.

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Pandoreas, Australian natives of horticultural significance, were successfully propagated using tissue culture. A protocol for rapid in vitro multiplication of commercial cultivars was developed using nodal segments cultured on Murashige and Skoog medium containing either BA or kinetin. Maximum shoot induction and number of shoots per explant for P. pandorana (Andrews) Steenis and P. jasminoides (Lindley) Schumann were on 8.8 μm BA and 4.6 μm kinetin. Higher levels of cytokinin in the medium inhibited shoot formation. Tissue-cultured shoots were rooted with IBA. This study demonstrates that Pa

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Ali-Ahmad, Masooma, and Harrison Hughes. "807 PB 141 LEAF EPICUTICULAR WAX OF IN VITRO GRAPE PLANTLETS TREATED WITH AND WITHOUT POLYETHYLENE GLYCOL." HortScience 29, no. 5 (1994): 548g—548. http://dx.doi.org/10.21273/hortsci.29.5.548g.

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Scanning electron microscopic (SEM) studies and gravimetric analysis of in vitro cultured leaf surfaces showed reduced epicuticular wax (EW) structurally and quantitatively as compared to greenhouse plants. However, leaves of in vitro plantlets subjected to polyethylene glycol-treatment (PEG) showed an increase in quantitative and structural EW which was similar to that of greenhouse plants. Furthermore, leaves initiated during in vitro culture and which persisted, when transferred to the greenhouse, showed an increase in structural wax as well as in amount, 30 days after transplanting in the

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Andrade, Evelyn R., Robert van den Hurk, Lívia A. Lisboa, et al. "Effects of ascorbic acid on in vitro culture of bovine preantral follicles." Zygote 20, no. 4 (2012): 379–88. http://dx.doi.org/10.1017/s0967199412000056.

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SummaryThe objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium (MEM) supplemented or not with various doses of ascorbic acid. Ovarian tissue was processed for histology, transmission electron microscopy (TEM) and immunohistochemical demonstration of prolifer

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Lacasse, J., C. Kreft, J. Gutkowska, J. Genest, and M. Cantin. "Cultured juxtaglomerular cells: production and localization of renin." Canadian Journal of Physiology and Pharmacology 65, no. 7 (1987): 1409–15. http://dx.doi.org/10.1139/y87-221.

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Trypsin- or collagenase-dispersed renal cortical cells from newborn mice were filtered and cultured. The cultures comprised 25% juxtaglomerular cells as identified by immunocytochemistry. Renin was measured by radioimmunoassay in the culture medium and in the cells at various time intervals. This in vitro system was responsive to isoproterenol, which stimulated renin release in a dose-dependent manner.

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Barroso, P. A. A., L. R. F. M. Paulino, B. R. Silva, et al. "Effects of dexamethasone on growth, viability and ultrastructure of bovine secondary follicles cultured in vitro." Zygote 28, no. 6 (2020): 504–10. http://dx.doi.org/10.1017/s0967199420000416.

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SummaryThis study aimed to evaluate the effects of dexamethasone on development, viability, antrum formation and ultrastructural integrity of bovine secondary follicles cultured in vitro for 18 days. Bovine ovaries were obtained from slaughterhouses and secondary follicles of ~150–200 µm diameter were isolated and cultured in the laboratory in TCM-199+ alone or supplemented with different concentrations of dexamethasone (1, 10, 100 and 1000 ng/ml). Follicle viability was evaluated after the culture period, using calcein-AM (viable) and ethidium homodimer (nonviable). Follicle diameters and ant

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Orihuela, Carlos J., Rob Janssen, Christopher W. Robb, David A. Watson, and David W. Niesel. "Peritoneal Culture Alters Streptococcus pneumoniae Protein Profiles and Virulence Properties." Infection and Immunity 68, no. 10 (2000): 6082–86. http://dx.doi.org/10.1128/iai.68.10.6082-6086.2000.

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ABSTRACT We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial ce

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Alves, A. M. C. V., R. N. Chaves, R. M. P. Rocha, et al. "Dynamic medium containing growth differentiation factor-9 and FSH maintains survival and promotes in vitro growth of caprine preantral follicles after long-term in vitro culture." Reproduction, Fertility and Development 25, no. 6 (2013): 955. http://dx.doi.org/10.1071/rd12180.

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The aim of the present study was to evaluate the effects of growth differentiation factor 9 (GDF-9) and FSH on the in vitro development of caprine preantral follicles cultured for 16 days. Ovarian fragments were cultured in αMEM+ (α-minimum essential medium, pH 7.2–7.4, 10 μg mL–1 insulin, 5.5 μg mL–1 transferrin, 5.0 ng mL–1 selenium, 2 mM glutamine, 2 mM hypoxanthine and 1.25 mg mL–1 bovine serum albumin) in the absence or presence of 200 ng mL–1 GDF-9 and/or 50 ng mL–1 FSH added during the first (Days 0–8) and/or second (Days 8–16) half of the culture period. Non-cultured and cultured fragm

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Lonergan, P., D. Rizos, A. Gutierrez-Adan, et al. "243TEMPORAL DIVERGENCE IN THE PATTERN OF MRNA EXPRESSION IN BOVINE EMBRYOS CULTURED FROM THE ZYGOTE TO BLASTOCYST STAGE IN VITRO OR IN VIVO." Reproduction, Fertility and Development 16, no. 2 (2004): 242. http://dx.doi.org/10.1071/rdv16n1ab243.

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The objective of this study was to examine the time during the post-fertilization culture period that gene expression patterns of in vitro cultured bovine embryos diverge from those of their in vivo cultured counterparts. Presumptive bovine zygotes were produced by IVM/IVF of immature oocytes collected from the ovaries of slaughtered animals. At approximately 20h post-insemination (hpi), presumptive zygotes were randomly divided into two culture groups, either in vitro in synthetic oviduct fluid or in vivo, and transferred into the ewe oviduct. Embryos were recovered from both systems at appro

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27

McKechnie, N. M., M. Boulton, H. L. Robey, F. J. Savage, and I. Grierson. "The cytoskeletal elements of human retinal pigment epithelium: in vitro and in vivo." Journal of Cell Science 91, no. 2 (1988): 303–12. http://dx.doi.org/10.1242/jcs.91.2.303.

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The cytoskeletal elements of normal (in situ) and cultured human retinal pigment epithelium (RPE) were studied by a variety of immunocytochemical techniques. Primary antibodies to vimentin and cytokeratins were used. Positive immunoreactivity for vimentin was obtained with in situ and cultured material. The pattern of reactivity obtained with antisera and monoclonals to cytokeratins was more complex. Cytokeratin immunoreactivity could be demonstrated in situ and in cultured cells. The pattern of cytokeratin expression was similar to that of simple or glandular epithelia. A monoclonal antibody

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Cott, G. R., J. Y. Westcott, and N. F. Voelkel. "Prostaglandin and leukotriene production by alveolar type II cells in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 258, no. 4 (1990): L179—L187. http://dx.doi.org/10.1152/ajplung.1990.258.4.l179.

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Alveolar type II cells were isolated from adult rats, cultured for 22 h, and individual eicosanoids in the media from unstimulated and stimulated cells were quantified by immunoassay. Stimulation with the calcium ionophore A23187 significantly increased the media levels of prostaglandins (prostaglandin and 6-keto-prostaglandin F1 alpha greater than thromboxane B2). In contrast to previous reports, increased media levels of leukotrienes were also recovered from cells incubated with A23187, but only for cells in culture for less than or equal to 24 h. The production of leukotriene C4 was confirm

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Arekar, Ashish R., Janhavi A. Arekar, S. S. Barve, and G. T. Paratkar. "In vitro regeneration of Momordica dioica (Roxb.)." Journal of Applied and Natural Science 4, no. 2 (2012): 297–303. http://dx.doi.org/10.31018/jans.v4i2.268.

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Momordica dioica, Roxb. (Family: Cucurbitaceae) commonly called as Kartoli, is an important medicinal plant, which has remained unexplored from the commercial point of view. Considering its scarce availability and the medicinal importance, in vitro cultures were established. Traditionally, M. dioica has been propagated mainly through its tuberous roots and less commonly by seeds. Germination through seeds is very difficult or impossible because of hard seed coat. As an alternative to traditional methods tissue culture offers an efficient method for propagation of M. dioica. Mature seeds were u

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Redel, B. K., L. D. Spate, A. N. Brown, and R. S. Prather. "97 SUPPLEMENTATION WITH FOLATE IN VITRO INCREASES TROPHECTODERM AND TOTAL CELL NUMBER IN IN VITRO-DERIVED PORCINE BLASTOCYSTS." Reproduction, Fertility and Development 24, no. 1 (2012): 161. http://dx.doi.org/10.1071/rdv24n1ab97.

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It is vital that improvements are made to current culture environments because in vitro culture systems are suboptimal compared with in vivo. A previous transcriptional profiling endeavour conducted by Bauer et al. (2010 Biol. Reprod. 83, 791–798) identified hundreds of mRNA transcripts that were mis-expressed in porcine embryos fertilized in vivo and then cultured in vitro to Day 6 compared with in vivo Day-6 embryos. Enriched in the downregulated transcripts were 4 genes involved with the one carbon pool by folate KEGG pathway. This downregulation of genes involved with folate metabolism may

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Edelman, P. D., D. C. McFarland, V. A. Mironov, and J. G. Matheny. "Commentary: In Vitro-Cultured Meat Production." Tissue Engineering 11, no. 5-6 (2005): 659–62. http://dx.doi.org/10.1089/ten.2005.11.659.

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Teryukova, N. P., G. I. Blinova, and V. A. Ivanov. "Zajdela hepatoma cells cultured in vitro." Cell and Tissue Biology 7, no. 3 (2013): 245–52. http://dx.doi.org/10.1134/s1990519x13030127.

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Marín, M. L., and J. A. Marín. "Excised rootstock roots cultured in vitro." Plant Cell Reports 18, no. 3-4 (1998): 350–55. http://dx.doi.org/10.1007/s002990050585.

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Sluss, Patrick M., Kan-ei Lee, John H. Mattox, Patricia C. Smith, Margaret C. Graham, and Ann B. Partridge. "Estradiol and progesterone production by cultured granulosa cells cryopreserved from in vitro fertilization patients." European Journal of Endocrinology 130, no. 3 (1994): 259–64. http://dx.doi.org/10.1530/eje.0.1300259.

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Sluss PM, Lee K, Mattox JH, Smith PC, Graham MC, Partridge AB. Estradiol and progesterone production by cultured granulosa cells cryopreserved from in vitro fertilization patients. Eur J Endocrinol 1994;130:259–64. ISSN 0804–4643 Gonadotropin-stimulated steroidogenesis was studied in cultured human granulosa-lutein cells obtained from patients undergoing procedures for in vitro fertilization. The impact of cryopreservation on cell function in vitro was studied. Granulosa cells obtained from in vitro fertilization patients were cultured in serum-supplemented medium or cryopreserved at −135°C fo

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Silva, T. F., S. L. Costa, E. P. Costa, J. D. Guimarães, and V. L. D. Queiroz-Castro. "Effect of somatotropin on survival and diameter of bovine preantral follicles." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 71, no. 5 (2019): 1445–52. http://dx.doi.org/10.1590/1678-4162-10602.

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ABSTRACT The aim of this study was to evaluate the effect of Recombinant bovine somatotropin (rbST) on survival and diameter of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovaries were collected from adult cows and fragments of ovarian cortex were immediately fixed (non-cultured control) or cultured in vitro in α-MEM+ alone or containing 10, 50, 100 or 1,000ng/mL rbST. The fragments were processed for Classical Histology and Transmission Electron Microscopy. After one and seven days of culture, the percentage of normal follicles in the non-cultured control was superior (P<

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RANKIN, KENNETH S., RACHEL L. LAKEY, CRAIG H. GERRAND, ANDREW P. SPROWSON, ANDREW W. McCASKIE, and MARK A. BIRCH. "A Novel in Vitro Model to Investigate Behavior of Articular Chondrocytes in Osteoarthritis." Journal of Rheumatology 37, no. 2 (2009): 426–31. http://dx.doi.org/10.3899/jrheum.080080.

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Objective. To investigate in vivo simulation of the microenvironment in which osteoarthritis (OA) chondrocytes are cultured in vitro.Methods. Human articular chondrocytes were cultured under normoxic and hypoxic conditions. Cells were cultured on standard culture plastic or a porous polyHEMA surface that closely resembles the in vivo cartilage microarchitecture. Morphological changes to the cells were demonstrated by fluorescent staining with DAPI and vinculin. Proteoglycan and type II collagen protein levels were assessed using established techniques. Matrix metalloproteinase-1 (MMP-1) produc

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Li, Ming, Yong-Hai Li, Yi Hou, Xiao-Fang Sun, Qingyuan Sun, and Wei-Hua Wang. "Isolation and culture of pluripotent cells from in vitro produced porcine embryos." Zygote 12, no. 1 (2004): 43–48. http://dx.doi.org/10.1017/s0967199404002679.

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The present study was designed to examine whether in vitro produced porcine embryos can be used to establish an embryonic stem (ES) cell line. Porcine embryos were produced by in vitro maturation and in vitro fertilization. Embryos at the 4-cell to blastocyst stages were cultured in an ES medium containing 16% fetal bovine serum with mouse embryonic fibroblasts as a feeder layer. It was found that ES-like colonies were derived only from blastocysts. When these ES-like colonies were separated in 0.25% trypsin–0.02% EDTA solution and cultured again, ES-like colonies were further observed in the

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Gupta, P. S. P., H. S. Ramesh, B. M. Manjunatha, S. Nandi, and J. P. Ravindra. "Production of buffalo embryos using oocytes from in vitro grown preantral follicles." Zygote 16, no. 1 (2008): 57–63. http://dx.doi.org/10.1017/s096719940700442x.

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SummaryThe present study examines the use of buffalo preantral follicles as a source of oocytes for in vitro embryo production. Preantral follicles were isolated from abattoir-derived buffalo ovaries and were grown for 100 days in five different culture systems: (1) minimum essential medium (MEM); (2) coconut water; (3) MEM + ovarian mesenchymal cell (OMC) co-culture; (4) MEM + granulosa cell (GC) co-culture; or (5) MEM + cumulus cell (CC) co-culture. Low growth rates for the preantral follicles were observed when follicles were cultured in MEM or coconut water medium. Moderate growth rates we

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Buchala, A. J., T. Genoud, and H. Meier. "Polysaccharides in the culture medium of cotton cells cultured in vitro." Food Hydrocolloids 1, no. 5-6 (1987): 359–63. http://dx.doi.org/10.1016/s0268-005x(87)80026-7.

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Carrara, Maria, Lorenzo Cima, Roberto Cerini, and Maurizio Dalle Carbonare. "A New In Vitro Model for Predicting the Toxicity of Cosmetic Products." Alternatives to Laboratory Animals 20, no. 1 (1992): 138–43. http://dx.doi.org/10.1177/026119299202000119.

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A method has been developed whereby cosmetic products which are not soluble in water or in alcohol can be brought into contact with cell cultures by being placed in a cell culture insert, which is then placed in the cell culture well. Preliminary experiments were carried out with L929 cells, and cytotoxicity was evaluated by measuring neutral red uptake and the total protein content of treated cultured cells. Encouraging results were obtained in comparisons of three cosmetic emulsions and of one emulsion containing a range of concentrations of two preservatives, Kathon CG and Bronopol.

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Villalobos, Victor M., Edward C. Yeung, and Trevor A. Thorpe. "Origin of adventitious shoots in excised radiata pine cotyledons cultured in vitro." Canadian Journal of Botany 63, no. 12 (1985): 2172–76. http://dx.doi.org/10.1139/b85-307.

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Previous studies on shoot initiation in cultured excised cotyledons of Pinus radiata indicated that the process began early in culture on the side of the cotyledon in contact with the cytokinin-containing medium. In contrast to cotyledons cultured without cytokinin, organized structures, which have been termed promeristemoids, can be observed in cotyledons cultured with cytokinin after 5 days in culture. These structures arise from single cells in the first subepidermal cell layer at day 3. They are stable and their continued division leads to the formation of meristemoids, shoot primordia, an

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Schinor, Evandro Henrique, Fernando Alves de Azevedo, Francisco de Assis Alves Mourão Filho, and Beatriz Madalena Januzzi Mendes. "In vitro organogenesis in some citrus species." Revista Brasileira de Fruticultura 33, no. 2 (2011): 526–31. http://dx.doi.org/10.1590/s0100-29452011005000050.

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In vitro organogenesis of Citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP - 0.0; 0.5; 1.0; 1.5 or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L-1 of BAP. The efficiency

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Lima-Verde, I. B., M. H. T. Matos, J. B. Bruno та ін. "Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured". Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61, № 1 (2009): 57–65. http://dx.doi.org/10.1590/s0102-09352009000100009.

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The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (contr

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Pizza, Francis X., Thomas J. McLoughlin, Stephen J. McGregor, Edward P. Calomeni, and William T. Gunning. "Neutrophils injure cultured skeletal myotubes." American Journal of Physiology-Cell Physiology 281, no. 1 (2001): C335—C341. http://dx.doi.org/10.1152/ajpcell.2001.281.1.c335.

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The purpose of the study was to test the hypothesis that neutrophils can injure cultured skeletal myotubes. Human myotubes were grown and then cultured with human blood neutrophils. Myotube injury was quantitatively and qualitatively determined using a cytotoxicity (51Cr) assay and electron microscopy, respectively. For the 51Cr assay, neutrophils, under non-in vitro-stimulated and N-formylmethionyl-leucyl-phenylalanine (FMLP)-stimulated conditions, were cultured with myotubes at effector-to-target cell (E:T) ratios of 10, 30, and 50 for 6 h. Statistical analyses revealed that myotube injury w

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Pellegrini, Graziella, Roberto Gherzi, Adriano Tito Franzi, Fiorella D'Anna, Michele De Luca, and Ranieri Cancedda. "In Vitro Reconstitution of Differentiated Human Epithelia." Alternatives to Laboratory Animals 20, no. 1 (1992): 95–102. http://dx.doi.org/10.1177/026119299202000113.

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Human keratinocytes obtained from skin biopsies can be serially cultured in vitro. When plated on lethally irradiated 3T3 fibroblasts, keratinocyte colonies reconstitute a stratified squamous epithelium devoid of stratum corneum. The expression of a mature cornified epidermis, expressing all the morphological and biochemical markers of the in vivo epidermis, can be obtained by the “emerged dermal equivalent” culture system. Melanocytes grown under the same culture conditions, maintain a physiological melanocyte/keratinocyte ratio, are organised in the basal layer of the cultured epidermis, and

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Jia, Zhidong, Yuan Cheng, Xinan Jiang, et al. "3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity." BioMed Research International 2020 (March 4, 2020): 1–22. http://dx.doi.org/10.1155/2020/6354183.

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In vitro 3D hepatocyte culture constitutes a core aspect of liver tissue engineering. However, conventional 3D cultures are unable to maintain hepatocyte polarity, functional phenotype, or viability. Here, we employed microfluidic chip technology combined with natural alginate hydrogels to construct 3D liver tissues mimicking hepatic plates. We comprehensively evaluated cultured hepatocyte viability, function, and polarity. Transcriptome sequencing was used to analyze changes in hepatocyte polarity pathways. The data indicate that, as culture duration increases, the viability, function, polari

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Mercer, J. G., A. E. Munn, J. W. Smith, and H. H. Rees. "Cuticle production and ecdysis in larval marine ascaridoid nematodes in vitro." Parasitology 92, no. 3 (1986): 711–20. http://dx.doi.org/10.1017/s0031182000065562.

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SUMMARYInfective 3rd-stage larvae of three species of marine ascaridoid nematodes, Pseudoterranova (= Phocanema = Porrocaecum = Terranova) decipiens, Anisakis simplex and Phocascaris/Contracaecum sp. were cultured in vitro in 0·9% saline or Medium 199 at 37°C beneath an atmosphere of either air or 95% air:5% CO2 All three species responded to culture at 37°C by producing a new cuticle. The majority of P. decipiens completed ecdysis under all the culture conditions employed. Ecdysis in A. simplex was stimulated by a high concentration of CO2; this effect was particularly noticeable in saline cu

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Yamauchi, N., H. Sasada, S. Sugawara, and T. Nagai. "Effect of culture conditions on artificial activation of porcine oocytes matured in vitro." Reproduction, Fertility and Development 8, no. 8 (1996): 1153. http://dx.doi.org/10.1071/rd9961153.

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The effects of culture media used and culture period for in vitro maturation of porcine oocytes on their subsequent response to chemical and electrical activation, were investigated. Activated oocytes were identified by the presence of a pronucleus(ei) or cleavage. Porcine oocytes were cultured for 24, 30, 36, 42 and 48 h in TCM199 with Earle's salts (199) supplemented with 10% fetal calf serum (199-FCS) before electrical stimulation. Although few oocytes were activated after 24 h and 30 h of culture (5.4% and 6.1% respectively), the percentage of activated oocytes increased significantly to 9

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Adelberg, J., M. Kroggel, and J. Toler. "Physical Environment in vitro Affects Laboratory and Nursery Growth of Micropropagated Hostas." HortTechnology 10, no. 4 (2000): 754–57. http://dx.doi.org/10.21273/horttech.10.4.754.

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Hosta ×hybrid Tratt. `Blue Cadet' and Hosta tokudama Tratt. `Newberry Gold' were micropropagated in shaken liquid culture and on agar media, in conventional vessels and vessels modified for ventilation in vitro. Acclimatization under intermittent mist and growth in an outdoor nursery during the late spring and summer were monitored by dry weight analysis of sample plants every 4 days for a 60-day period (ex vitro growth). Results for `Newberry Gold' were 1) in vitro shoot growth was greater in liquid than agar culture, regardless of vessel; 2) shoots from agar or liquid culture grew at similar

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Tablin, F., M. Castro, and R. M. Leven. "Blood platelet formation in vitro. The role of the cytoskeleton in megakaryocyte fragmentation." Journal of Cell Science 97, no. 1 (1990): 59–70. http://dx.doi.org/10.1242/jcs.97.1.59.

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We have developed a unique in vitro model that promotes differentiation of megakaryocytes into platelets. When megakaryocytes isolated from guinea pig bone marrow were cultured on hydrated rat tail collagen gels, cells spontaneously formed elongated, beaded processes that fragmented to yield cytoplasmic pieces with the same size and internal composition as individual platelets. Addition of nocodazole at the initiation of cultures blocked process formation, while addition of nocodazole to cells with previously established processes resulted in their retraction. The addition of taxol to cultures

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