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Journal articles on the topic 'In vitro gas digestibility technique'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Olivo, Paula Martins, Geraldo Tadeu dos Santos, Luís Carlos Vinhas Ítavo, Ranulfo Combuca da Silva Junior, Eduardo Souza Leal, and Rodolpho Martin do Prado. "Assessing the nutritional value of agroindustrial co-products and feed through chemical composition, in vitro digestibility, and gas production technique." Acta Scientiarum. Animal Sciences 39, no. 3 (2017): 289. http://dx.doi.org/10.4025/actascianimsci.v39i3.34024.

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Agroindustrial co-products are a viable alternative for use in animal nutrition. Tests were conducted using eight different types of co-products and feed to evaluate the chemical composition, in vitro digestibility of dry matter, crude protein and neutral detergent fiber, and gas production by them. The co-products tested were: coffee hulls; pelleted citrus pulp; grape residue; soybean hulls; cottonseed; cassava foliage; and foods usually supplied to ruminants: corn silage and ground corn concentrate. Data of in vitro digestibility of dry matter, crude protein and neutral detergent fiber were

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Mauricio, R. M., E. Owen, M. S. Dhanoa, and M. K. Theodorou. "Comparison of rumen liquor and faeces from cows as sources of micro-organisms for the in vitro gas production technique." BSAP Occasional Publication 22 (1998): 182–84. http://dx.doi.org/10.1017/s0263967x00032523.

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Bioassay methods to estimate the digestibility of forages for ruminants, such as the in vitro digestibility technique (Tilley and Terry, 1963), the nylon bag technique (Ørskov et al., 1980) and the gas production methods of Menke and Steingass (1988) and Theodorou et al. (1994), require rumen fistulated animals, either to provide a suitable in situ environment or to provide rumen liquor as a source of inoculum. Not only is establishing and maintaining fistulated animals expensive, but fistulation is an invasive technique which is increasingly discouraged on animal welfare grounds. There is the

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Kitessa, S., G. G. Irish, and P. C. Flinn. "Comparison of methods used to predict the in vivo digestibility of feeds in ruminants." Australian Journal of Agricultural Research 50, no. 5 (1999): 825. http://dx.doi.org/10.1071/ar98169.

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Digestibility is a very useful index of the energy content of ruminant feeds, but cheaper and quicker laboratory methods are required as an alternative to the ultimate measure of in vivo digestibility using animals. These methods involve either prediction of digestibility from chemical composition or in vitro and in situ simulation of the digestion process. This review presents a range of chemical and in vitro techniques for predicting digestibility, together with an assessment of their advantages and limitations, particularly the degree to which they account for the sources of variation in in

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Cherdthong, Anusorn, Rittikeard Prachumchai, Chanadol Supapong, et al. "Inclusion of yeast waste as a protein source to replace soybean meal in concentrate mixture on ruminal fermentation and gas kinetics using in vitro gas production technique." Animal Production Science 59, no. 9 (2019): 1682. http://dx.doi.org/10.1071/an18491.

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This experiment was conducted to investigate the utilisation of yeast waste as protein source to replace soybean meal in concentrate mixture on kinetic of gas, rumen ammonia-nitrogen and digestibility of nutrients by using in vitro gas production technique. The experimental design was a completely randomised design and the dietary treatments were replacing soybean meal with yeast wastein concentrate at the ratio of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. Yeast waste was obtained from KSL Green Innovation Public Co. Limited, Thailand. The gas production was recorded at 0, 0.5, 1, 2,

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Filho, S. L. S. Cabral, I. C. S. Bueno, E. F. Nozella, A. L. Abdalla, and D. M. S. S. Vitti. "Tannin bioassay using semi-automated and manual gas production techniques for Brazilian browses." Proceedings of the British Society of Animal Science 2003 (2003): 191. http://dx.doi.org/10.1017/s1752756200013508.

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Native herbaceous browses at Northeast of Brazil have a dry tolerance and have been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on their intake and digestibility. Polyethylene glycol (PEG) has been used in gas-based techniques for assessing anti nutritional factors in tanniniferous plants for ruminants. The objective of this work was to compare the tannin bioassay technique using the semi-automated (Reading Pressure Technique - RPT) and manual (syringe) gas production techniques to evaluate the tannin effect upon in vitro rumen f

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Plaizier, J. C., and S. Li. "Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique." Canadian Journal of Animal Science 93, no. 3 (2013): 399–402. http://dx.doi.org/10.4141/cjas2012-153.

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Plaizier, J. C. and Li, S. 2013. Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique. Can. J. Anim. Sci. 93: 399–402. The potential of the gas production technique to predict in vitro dry matter digestibility was determined using 50 ruminant feeds, including grass/alfalfa forages, grain crop silages, grains, high protein feeds, and feed straws. Without including the chemical composition in the prediction equation, the prediction of IVDMD based on the cumulative gas production during 48 h alone

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7

Ansari, A., A. Taghizadeh, H. Janmohamadi, and G. Zarini. "Effects of Saccharomyces cerevisiae on the nutritive value of lucerne hay assessed with the in vitro gas production technique." Proceedings of the British Society of Animal Science 2009 (April 2009): 186. http://dx.doi.org/10.1017/s1752756200030258.

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Yeast culture (Saccharomyces cerevisiae) has been widely used as a dietary supplement in ruminants. Saccharomyces cerevisiae supplementation in ruminant diets can increase dry matter intake, production performance, cellulose degradation, and nutrient digestibility (Callaway and Martin, 1997). In vitro techniques are routinely used to evaluate the nutritional quality of feeds because of their convenience, adaptability and efficiency. The in vitro gas production technique is an accurate and fast method to evaluate the nutritive value of feeds and it can be a good alternative method instead of in

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Romney, D. L., F. C. Cadario, E. Owen, and A. H. Murray. "Comparison of parameters from the Theodorou gas production technique using nitrogen-free and nitrogen-rich media as predictors of dry-matter intake and digestibility." BSAP Occasional Publication 22 (1998): 172–74. http://dx.doi.org/10.1017/s0263967x00032493.

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Parameters from in vitro gas production techniques could have potential as predictors of dry-matter intake (DMI) and digestibility. Fermentation is usually carried out under conditions where nitrogen (N) is not limiting. Therefore where N supply is a constraint to intake and digestibility, prediction equations may be inaccurate. This study compared the use of N-free and N-rich media in an in vitro fermentation method (Theodorou et al., 1994) and studied the relationships between in vitro and in vivo parameters obtained using both media.

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Furtado, C. E., D. M. S. S. Vitti, I. C. S. Bueno, et al. "Gas production technique in the evaluation of horse feeds using equine faeces and rumen liquid as inoculum source 2. In vitro digestibility." Proceedings of the British Society of Animal Science 2005 (2005): 111. http://dx.doi.org/10.1017/s175275620001022x.

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In vitro and in situ techniques for research on ruminants are currently much in focus. Since they have good correlations with in vivo data, they are feasible alternatives to predict the nutrition rates of feeds and may be applied in equine research on in vivo apparent digestibility. On the other hand, the disadvantage of these methods is due to the fact that fistulated animals are required to obtain the inoculum. Theodorou et al., (1994) developed an extremely promising gas production technique to assess feeds for ruminants, but still require rumen inoculum obtained from operated animals. Faec

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Minson, D. J. "A history ofin vitrotechniques." BSAP Occasional Publication 22 (1998): 13–19. http://dx.doi.org/10.1017/s0263967x00032213.

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AbstractRegressions relating in vivo digestibility to chemical composition of the food have residual standard deviations that are unacceptably high. The development of the two-stage in vitro technique inoculated with rumen liquor (Tilley and Terry, 1963) allowed dry-matter digestibility to be predicted with greater accuracy. This success was followed by a series of developments which replaced rumen liquor with inoculum produced from fresh or preserved faeces collected from sheep or cattle. Other methods used inoculum from a continuous fermentation containing rumen micro-organisms and enzymes p

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Meads, N. D., R. Tahmasbi, and N. Jantasila. "The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey." Journal of Applied Animal Nutrition 9, no. 2 (2021): 99–103. http://dx.doi.org/10.3920/jaan2021.0006.

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Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260

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Nozella, E. F., S. L. S. Cabral Filho, I. C. S. Bueno, et al. "Tannin bioassay using semi-automated gas production technique." Proceedings of the British Society of Animal Science 2005 (2005): 218. http://dx.doi.org/10.1017/s1752756200011297.

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Brazil has arid regions where livestock production is limited by forage source. However, some native herbaceous browses have a dry tolerance and had been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on intake and digestibility. Tannins have a high affinity to proteins and could make these molecules unavailable for animal. Compounds as polyethylene glycol (PEG) has been used on tannin studies, because it has more affinity with tannins than proteins. Based on that, it is possible to evaluate the nutritive potential of tanniniferous

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Longland, A. C., S. P. Bray, A. E. Brooks, M. K. Theodorou, and A. G. Low. "Relationship between in vitro gas production and in vivo energy digestibility in growing pigs." Proceedings of the British Society of Animal Science 1996 (March 1996): 142. http://dx.doi.org/10.1017/s0308229600031093.

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In vitro methods which can be used to predict the nutritive value of feedstuflfs for livestock are attractive in terms of both speed and economy. The in vitro pressure transducer technique (PTT) whereby the gas evolved during the in vitro fermentation of feedstuffs in rumen fluid is quantified by a pressure transducer, has been used to predict the nutritive value of ruminant feedstuffs. Here the potential for the PTT in predicting the digestible energy contents of eight feedstuffs varying in non-starch polysaccharide (NSP) content fed to growing pigs, was investigated.

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Sousa, Luciano Fernandes, Jhone Tallison Lira de Sousa, Érica Beatriz Schultz, and Gilberto de Lima Macedo Júnior. "Babassu mesocarp meal for ewe lambs feeding: In vitro ruminal fermentation and in vivo apparent digestibility." Acta Scientiarum. Animal Sciences 43 (August 19, 2020): e51056. http://dx.doi.org/10.4025/actascianimsci.v43i1.51056.

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The purpose of the current study was to evaluate the in vitro fermentation (IVRF) and apparent in vivo digestibility (AIVD) of diets formulated with four inclusion levels of babassu mesocarp meal (BMM) (0.0; 7.5; 15.0 and 22.5%) in ewe lambs. The IVRF test was performed through the "Hohenheim Gas Test" technique, following a randomized complete block design, with four blocks (rumen fluids from four different bovines). Gas production was measured at 3, 6, 9, 12, 24, 48, 72, and 96 hours after incubation. For the AIVD trial, 20 ewe lambs were used in a completely randomized design with five repl

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Palic, Dragan, and Klaas-Jan Leeuw. "Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants." Acta Periodica Technologica, no. 40 (2009): 79–86. http://dx.doi.org/10.2298/apt0940079p.

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In this study, the organic matter digestibility (OMD) of six complete diets for ruminants has been determined in-vivo in trials with sheep and in-vitro using two-stage Tilley and Terry (T&T) method, gas production (GP) technique and multi-enzyme incubation (EDOM) procedures. The mean OMD values obtained in vivo and using T&T, GP and EDOM techniques were 684, 716, 685 and 710 g OM/kgDM respectively and did not differ significantly (P>0.05). The obtained in vitro results were regressed against determined in-vivo values to derive prediction equations. Using the T&T technique, the p

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Yammuen-art, S., P. Somrak, and C. Phatsara. "Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle." Animal Production Science 57, no. 8 (2017): 1603. http://dx.doi.org/10.1071/an15692.

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The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96

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Yammuen-art, S., P. Somrak, and C. Phatsara. "Corrigendum to: Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle." Animal Production Science 59, no. 3 (2019): 600. http://dx.doi.org/10.1071/an15692_co.

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The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96

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Rajtar, Patrycja, Paweł Górka, Tomasz Schwarz, and Piotr Micek. "Effect of Hybrid Rye and Maize Grain Processing on Ruminal and Postruminal Digestibility Parameters." Annals of Animal Science 20, no. 3 (2020): 1065–83. http://dx.doi.org/10.2478/aoas-2020-0025.

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AbstractThe aim of the study was to determine the effect of the method of fragmentation of hybrid rye and maize grain on digestibility parameters for ruminants. Varying degrees of fragmentation – none (whole grains; WG), crushed (CG) or ground to pass through a 4.0 (GG4.0) or 1.5 mm (GG1.5) screen – were estimated by the in sacco nylon bag technique, in vitro true digestibility (IVTD), and the in vitro gas production (GP) technique. WG, CG, GG4.0 and GG1.5 were categorized as none, slight, moderate and extensive fragmentation of the grain kernel, respectively. Three non-lactating cows fitted w

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Kurniawati, Asih, Lies Mira Yusiati, Widodo Widodo, and Wayan Tunas Artama. "Study of Local Herb Potency as Rumen Modifier: Red Ginger (Zingiber Officinale Var. Rubrum) Addition Effect on In Vitro Ruminal Nutrient Digestibility." ANIMAL PRODUCTION 21, no. 1 (2020): 30. http://dx.doi.org/10.20884/1.jap.2019.21.1.713.

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Red ginger is herb that commonly used as part of traditional medicine due to its essential oil content. Some essential oil have proven as rumen fermentation modifier. Addition of red ginger in ruminant diet was studied using in vitro gas production technique to evaluate its effect on nutrient digestibility. Red ginger meal was added to meet essential oil level in fermentation medium of 0 mg/l as control, 25, 50, 75 and 100 mg/l. The diet consisted of Penisetum hybride, rice bran, wheat pollard in ratio 60:20:20 DM basis. Feed fermentation was incubated for 24 h at 39°C. At the end of incubatio

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Adesogan, A. T., D. I. Giverts, and E. Owen. "A Comparison between in vitro digestibility, in situ degradability and a gas production technique for predicting the in vivo digestibility of whole crop wheat." Proceedings of the British Society of Animal Science 1995 (March 1995): 118. http://dx.doi.org/10.1017/s0308229600028841.

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Public aversion to animal experimentation demands the replacement of typically expensive, laborious and lengthy in vivo forage evaluation techniques with non-invasive, laboratory-based techniques. This study aimed to assess the suitability of three less animal-dependent techniques (in vitro digestibility (Tilley and Terry, 1963), in situ degradability (Ørskov and McDonald, 1979) and gas production (Theodorou et al., 1994)) for predicting the in vivo digestibility of whole crop wheat (WCW). The study forms part of an on-going MAFF LINK project that aims to develop equations for the laboratory-b

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Morrin, M. J., J. A. Rooke, N. W. Offer, and F. D. deB Hovell. "Prediction of the Voluntary Intake of Grass Silages by Sheep from Rumen Degradation Characteristics measured by the Polyester Fibre Bag or by Gas Production." Proceedings of the British Society of Animal Production (1972) 1994 (March 1994): 118. http://dx.doi.org/10.1017/s0308229600026659.

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Various studies have reported that the voluntary intake (VFI) of hays (Hovell, Ngambi, Barber & Kyle. 1986, Khazaal, Dentinho, Ribeiro & 0rskov. 1993) and straws (Ørskov, Reid & Kay 1988, Blummel & Ørskov, 1993) is predicted better by rumen degradation characteristics than by whole tract digestibility. Prediction of grass silage VFI is in general poor but is related to both whole tract digestibility and silage fermentation characteristics. However no attempts have been made to relate silage VFI to rumen degradation characteristics. It was the purpose of this study to attempt to

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Kirkhope, R. T. S., and R. S. Lowman. "Use of an in vitro gas production technique with faeces as inoculant to assess tropical forage quality for equids." Proceedings of the British Society of Animal Science 1996 (March 1996): 248. http://dx.doi.org/10.1017/s0308229600032116.

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In tropical areas reliable estimation of feedstuff nutritional value often proves difficult. Recording in vitro gas production from feedstuffs, using caecal digesta as a gut microbial source, is a promising new method for analysing digestion kinetics in equids. However, this demands caecal fistulation. Substituting caecal fluid with faeces provides a potential non-invasive alternative.Naked Oats (NO) and four forages (Grass Pellets (GP), Alfalfa Hay (AH), Oat Straw (OS) and Millet Stover (MS)), of known in-vivo digestibility, were incubated in vitro with either a faecal or a caecal digesta ino

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Dagaew, Gamonmas, Anusorn Cherdthong, Metha Wanapat, and Pin Chanjula. "In vitro rumen gas production kinetics, hydrocyanic acid concentration and fermentation characteristics of fresh cassava root and feed block sulfur concentration." Animal Production Science 60, no. 5 (2020): 659. http://dx.doi.org/10.1071/an18784.

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Context Feeding ruminants with fresh cassava roots (FCR) is limited because they have a high concentration of hydrocyanic acid (HCN). Thus, it was hypothesised that receiving a feed block containing high sulfur (FBS) would reduce hydrocyanic acid (HCN) in FCR and improve rumen fermentation and nutrient digestibility in animals. Aims The goal of the present work was to study the influence of the ratio of FCR to rice straw (RS) together with FBS on kinetics of gas production, HCN concentration, fermentation characteristics and nutrient digestibility, using in vitro technique. Methods The experim

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Aregheore, E. M., and S. A. Abdulrazak. "Estimation of Organic matter digestibility and metabolizable energy content of Agro-industrial wastes using in vitro gas production." Nigerian Journal of Animal Production 32, no. 1 (2021): 79–87. http://dx.doi.org/10.51791/njap.v32i1.1046.

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Organic matter (OM), neutral detergent fibre (NDF) digestibilities and metabolizableenergy content of some available agro-chemicals wastes (AIW) such as dry brewer's grain cocoa shell, cocoa dust, dessicated cocnut waste meal, wheat bran and rice bran used as ruminant feeds were setimated from in vitro gas production technique. Their crude protein (CP) content varieted from 13.7-25.6%, NDF ranged from 21.9-48.4% Om from 88.6-95.4% and non structural carbohydrates (NSC) estimates increased from 7.2 - 45.6%. Dessicated coconut waste meal(DCWM) had the lowest NSC content. Nutrient contents varied

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Lister, S. J., M. S. Dhanoa, J. L. Stewart, and M. Gill. "Relationship between in vitro gas production and near infrared reflectance spectra of gliricidia provenances." Proceedings of the British Society of Animal Science 1996 (March 1996): 226. http://dx.doi.org/10.1017/s0308229600031895.

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The chemical composition of tropical forages is generally more complex than that of temperate forages, yet the need for simple methods to predict nutritive value is greater. Near Infrared Reflectance (NIR) spectra could provide a possible solution. NIR has been used to predict both in vitro and in vivo digestibility (Murray, 1993). Direct calibration with in vivo parameters is ideal, but factors such as different types of forages, animals and management conditions have to be taken into consideration. The gas production technique, which may be considered as an in vitro counterpart of the dacron

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Saliu, L. O., and T. O. Ososanya. "In vitro gas production and dry matter degradability of cassava top and maize stover mixture ensiled with Albizia saman pods." Nigerian Journal of Animal Production 44, no. 2 (2020): 187–94. http://dx.doi.org/10.51791/njap.v44i2.1022.

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In vitro gas production is an indication of microbial degradability of feed samples. Thus varying levels of mixture of cassava top (CT) and maize stover (MS) ensiled with Albizia saman pods (ASP) were examined. The feedstuff were dried and milled for gas determination in a completely randomized design. Samples were incubated using in vitro gas production technique. Gas production was measured at 3, 6, 9, 12, 15, 18, 21 and 24 h post incubation to estimate total gas volume (TGV), methane (CH4), metabolisable energy (ME; MJ/Kg DM), organic matter digestibility (OMD; %) and short chain fatty acid

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Khazaal, K., M. T. Dentinho, J. M. Ribeiro, and E. R. Ørskov. "A comparison of gas production during incubation with rumen contents in vitro and nylon bag degradability as predictors of the apparent digestibility in vivo and the voluntary intake of hays." Animal Science 57, no. 1 (1993): 105–12. http://dx.doi.org/10.1017/s0003356100006668.

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AbstractTen hays harvested at three stages (early bloom MB, mid bloom MB or in seed) made from lucerne (Medicago sativaj, sweet clover (Melilotus segetalis), Persian clover (Trifolium resupinatum) and pre-bloom (PB) Italian ryegrass (Lolium multiflorum var.), were offered ad libitum to four Merino male sheep and daily intake (g dry matter (DM) per kg M0·75) and DM apparent digestibility (DMD) were measured. In sacco DM degradation (g per 100 g DM), gas production (ml per 200 mg DM), in vitro digestibility and fibre composition (g/kg DM) of the hays were also studied. Gas production or DM degra

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Antunes Stella, L., V. Rosa Prates, A. Zubieta, C. Bayer, and J. O. Jardim Barcellos. "Óleos essenciais como modificadores da fermentação ruminal em substituição a monensina sódica in vitro." Archivos de Zootecnia 68, no. 264 (2019): 576–81. http://dx.doi.org/10.21071/az.v68i264.4998.

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The objective of this study to evaluate the effect of secondary plant compounds present in essential oils in replacement of monensin on in vitro ruminal fermentation parameters. It was adopted a completely randomized design with nine treatments and four replicates. The treatments were: control (CON), monensin (MON), garlic oil (ALH), cinnamon oil (CAN), clove oil (CRA), mint oil (HOR), juniper oil (JUN), bitter orange oil (LAR), and melaleuca oil (MEL). The in vitro gas technique was used to record total gas production at 4, 8, 12 and 24 h after incubation. MON, CAN and CRA increased gas produ

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Mc Geough, E. J., P. O'Kiely, M. O'Brien, and D. A. Kenny. "An evaluation of the methane output associated with high-moisture grains and silages using the in vitro total gas production technique." Animal Production Science 51, no. 7 (2011): 627. http://dx.doi.org/10.1071/an10243.

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This study aimed to quantify the methane and total gas emissions associated with a range of ensiled feeds using the in vitro total gas production (TGP) technique. This suite of feeds included cereal grains (wheat, barley and triticale) and maize, whole-crop wheat and grass silages. The methane and total gas output of these feeds was then regressed on chemical composition to assess the relationship between these variables. Subsequently, the efficacy of the TGP technique was also discussed. From this analysis it was observed that 96% of the variation in methane output per unit of feed incubated

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Besharati, M., A. Taghizadeh, and A. Ansari. "Effect of adding different levels of probiotic on in vitro gas production." Proceedings of the British Society of Animal Science 2009 (April 2009): 187. http://dx.doi.org/10.1017/s175275620003026x.

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Probiotics present an attractive alternative to the use of chemical and hormonal promoters in the livestock growth production industry. Preparations that contain micro-organisms have been safely used for many years and are generally accepted by both the farmer and the final consumer. Saccharomyces cerevisiae supplementation in ruminant diets can increase DMI, production performance, cellulose degradation, and nutrient digestibility (Callaway and Martin, 1997). The gas measuring technique has been widely used for the evaluation of nutritive value of feeds. Gas measurement provides useful data o

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Kang, Sungchhang, and Metha Wanapat. "Rumen-buffering capacity using dietary sources and in vitro gas fermentation." Animal Production Science 58, no. 5 (2018): 862. http://dx.doi.org/10.1071/an15466.

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The present study aimed to compare the effect of the rumen-buffering capacity of dietary sources and urea supplementation on ruminal kinetic gas production, rumen pH and fermentation efficiency and degradability, using in vitro gas-production techniques. The treatments were arranged according to a 4 × 3 factorial arrangement in a completely randomised design. The first factor was a rumen-buffering source, including the following: no buffering, and supplementation of 2% of sodium bicarbonate (NaHCO3), 2% of banana flower powder I (BAFLOP-I; Musa (AAA group)) or 2% of BAFLOP-II (Musa sapientum L

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Silva, Aghata, Aaron Norris, Arturo Franco, Felipe H. De Moura, and Mozart Fonseca. "PSIX-11 In vitro fermentation parameters of defatted hemp samples at different levels of irrigation." Journal of Animal Science 98, Supplement_4 (2020): 422–23. http://dx.doi.org/10.1093/jas/skaa278.736.

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Abstract The objective of this study was to determine the influence of defatted hemp under three irrigation regimes (0, 100, and 200 mm: DF0, DF1, and DF2 respectively) on in vitro fermentation parameters relative to alfalfa (Medicago sativa). Fermentation kinetics was accessed through in vitro gas production technique. Two ruminally cannulated Angus steers were used as inoculum donors in four incubation runs. For each incubation, four 160-mL serum bottles containing 200 mg of air-dried samples were incubated in 14 mL of buffering media and 4 mL of rumen inoculum. Each incubation spanned 48h w

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Adesogan, A. T., E. Owen, and D. I. Givens. "A comparison between in vitro digestibility, in situ degradability and a gas production technique for predicting the in vivo digestibility of whole-crop wheat." BSAP Occasional Publication 22 (1998): 33–35. http://dx.doi.org/10.1017/s0263967x00032237.

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Several published reports on the nutritive value of whole-crop wheat (WCW) have been based on estimations from laboratory techniques, some of which were developed for grass silage. However, there is little information on the accuracy of such estimations. Therefore the aim of this study was to evaluate the suitability of predicting the in vivo digestibility of WCW from various less animal-dependent techniques.

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Suharlina, S., D. A. Astuti, N. Nahrowi, A. Jayanegara, and L. Abdullah. "IN VITRO EVALUATION OF CONCENTRATE FEED CONTAINING Indigofera zollingeriana IN GOAT." Journal of the Indonesian Tropical Animal Agriculture 41, no. 4 (2016): 196. http://dx.doi.org/10.14710/jitaa.41.4.196-203.

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This study was conducted to evaluate the fermentability and nutrient digestibility of concentrate feed containing Indigofera zollingeriana using in vitro technique. Randomized complete block design was used on 3 types of concentrate feed and 4 groups (replicates) of goat's rumen liquid. The concentrate feeds were R1, R2 and R3 containing I. zollingeriana at 0 (control), 20 and 40%, respectively. Variables observed were total gas production, in vitro dry matter digestibility (IVDMD), organic matter (IVDOM), and crude protein (IVDCP), volatile fatty acid (VFA) profiles and NH3 concentration. Dat

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Freiria, Lucien Bissi da, Joanis Tilemahos Zervoudakis, Nelcino Francisco de Paula, et al. "Kinetic parameters of ruminal degradation in vitro with combinations of exogenous enzymes in diets of production systems simulated." Revista Brasileira de Saúde e Produção Animal 19, no. 1 (2018): 69–82. http://dx.doi.org/10.1590/s1519-99402018000100007.

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SUMMARY This study evaluated the effects of eight combinations of three exogenous enzymes with fibrolytic activity (FIB), amylolytic activity (AMZ), and proteolytic activity (PRO) on the accumulated gas production (PG) and kinetic parameters in simulated fermentations in two diet production systems using the in vitro gas production technique. In experiment 1, ruminal fluid was obtained from two Santa Ines sheep, cannulated in the rumen, kept in a feedlot and fed a diet containing a roughage:concentrate ratio of 20:80. In experiment 2, the ruminal fluid was obtained from two Santa Ines sheep, c

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Freiria, Lucien Bissi da, Joanis Tilemahos Zervoudakis, Nelcino Franciso de Paula, et al. "Do fibrolytic, proteolytic and amylolytic enzymes influence the in vitro fermentation characteristics of forage?" Semina: Ciências Agrárias 39, no. 3 (2018): 1143. http://dx.doi.org/10.5433/1679-0359.2018v39n3p1143.

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The effects of increasing doses of three exogenous enzymes preparations with fibrolytic activity (FIB - 0, 0.6, 1.2, 1.8, and 2.4 mg mL-1liquid volume incubated), amylolytic activity (AMZ - 0, 0.05, 0.10, 0.15, and 0.20 mg mL-1liquid volume incubated), and proteolytic activity (PRO - 0, 0.05, 0.10, 0.15, and 0.20 mg mL-1 liquid volume incubated ) on gas production (GP), kinetic parameters, and fermentation profile of Brachiaria brizantha cv. Marandu were evaluated using the in vitro gas production technique. Ruminal liquid was obtained from two rumen-cannulated Santa Inês sheep maintained on p

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Ayasan, Tugay, Ismail Ulger, Ayse Nuran Cil, Vincenzo Tufarelli, Vito Laudadio, and Valiollah Palangi. "Estimation of chemical composition, in vitro gas production, metabolizable energy, net energy lactation values of different peanut varieties and line by Hohenheim in vitro gas production technique." Semina: Ciências Agrárias 42, no. 2 (2021): 907–20. http://dx.doi.org/10.5433/1679-0359.2021v42n2p907.

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This study was carried out to determine the nutritional value of some selected peanut varieties and line in the Eastern Mediterranean Agricultural Research Institute of Adana, Turkey. The peanut varieties used were Gazipasa, Sultan, NC7, Cihangir, and Halisbey; while the peanut line was DA335/2011. The chemical composition, metabolizable energy (ME), net energy lactation (NEL) and organic matter digestibility (OMD) of the selected peanut varieties and line were determined through Hohenheim in vitro gas production technique. Incubation times for Hohenheim gas production technique were 3, 6, 9,

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38

Pendong, A., J. H. T. Barbi, E. Owen, and E. R. Deaville. "Prediction of in vivo digestibility of forages using in vitro techniques: comparison of the two-stage Tilley & Terry method with a gas production method." Proceedings of the British Society of Animal Science 1996 (March 1996): 223. http://dx.doi.org/10.1017/s030822960003186x.

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During the past twenty years the in vitro, two-stage digestibility method of Tilley and Terry (1963) has been widely used to predict the in vivo digestible organic matter content (DOMD) of forage dry matter. Recently in vitro gas production methods have attracted considerable research interest because of their potential to simulate the fermentation kinetics of forages in the rumen. However the potential of gas production methods to assess DOMD, as well as fermentation kinetics, has not been reported.

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Nsahlai, I. V., and N. N. Umunna. "Comparison between reconstituted sheep faeces and rumen fluid inocula and between in vitro and in sacco digestibility methods as predictors of intake and in vivo digestibility." Journal of Agricultural Science 126, no. 2 (1996): 235–48. http://dx.doi.org/10.1017/s0021859600073196.

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SUMMARYThis study (conducted in Debre Zeit, Ethiopia in 1993) examined (i) the effect of source of inoculum on in vitro dry matter (DM) digestibility (1VDMD) and gas production (GP) and (ii) the IVDMD, GP and in sacco degradability as predictors of in vivo DM digestibility (DMD) and intake. Six ruminally cannulated male sheep (used in the degradability studies and from which rumen fluid was harvested for the in vitro studies) and six intact sheep (from which faeces for reconstitution was obtained) were given teff straw ad libitum supplemented with 200 g/day of concentrate (1:1 mixture of noug

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Fernández-Rivera, S. "Relationships between gas release in vitro and in vivo quality measures of tropical forages." BSAP Occasional Publication 22 (1998): 36–39. http://dx.doi.org/10.1017/s0263967x00032249.

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Robust techniques that predict accurately and precisely the quality of forages are needed for developing improved feeding strategies and screening of large numbers of forage samples in breeding programmes. The release of gas in vitro (Menke et al., 1979) is attractive for this purpose because it does not require filtration and one single sample can be used to examine the kinetics of fermentation. The objective of this study was to determine the relationships between gas release in vitro and digestibility in vivo and intake of tropical forages.

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Khazaal, K., M. T. Dentinho, J. M. Ribeiro, and E. R. Ørskov. "Prediction of apparent digestibility and voluntary intake of hays fed to sheep: comparison between using fibre components, in vitro digestibility or characteristics of gas production or nylon bag degradation." Animal Science 61, no. 3 (1995): 527–38. http://dx.doi.org/10.1017/s1357729800014107.

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AbstractThe study compared using chemical components (i.e. crude protein (CP), neutral- and acid-detergent fibre or lignin (NDF, ADF and ADL) g/kg dry matter (DM), the in vitro digestibility (Tilley and Terry, 1963), the in situ (nylon bag) DM degradation (g/100 g DM) and gas production (ml/200 mg DM) techniques to predict voluntary daily intake (g DM per kg M0.75) and in vivo apparent DM digestibility (DMD) of 10 graminaceous hays individually offered ad libitum to four Merino male sheep. Gas production or DM degradation were determined after 6, 12, 24, 48, 72 or 96 h incubation and their cha

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Wood, C. D., N. S. Prathalingam, A. M. Murray, and R. W. Matthewman. "Use of the gas production technique to investigate the supplementation of nitrogen-deficient foods." BSAP Occasional Publication 22 (1998): 202–4. http://dx.doi.org/10.1017/s0263967x00032596.

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A major focus for improving the diets in many less developed countries (LDCS) is the provision of rumen fermentable nitrogen (N) using protein supplements to complement N-deficient foods. However, in vitro digestibility methods usually use N-rich environments for the degradation of single foods. This conventional approach may give data which do not reflect the nutritive value of the N-deficient diets often on offer in LDCS, neither is it appropriate for using in vitro gas production to study protein supplementation. Our earlier study indicated that, by using a N-free medium, the gas production

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Suntara, Chanon, Anusorn Cherdthong, Suthipong Uriyapongson, Metha Wanapat, and Pin Chanjula. "Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production." Journal of Fungi 6, no. 3 (2020): 109. http://dx.doi.org/10.3390/jof6030109.

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The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor

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Stotz, Miranda K., Sebastian E. Mejia-Turcios, Andrea M. Osorio, et al. "14 Effect of fermentation temperature on in vitro digestibility of a finishing diet." Journal of Animal Science 97, Supplement_1 (2019): 12. http://dx.doi.org/10.1093/jas/skz053.026.

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Abstract Heat stress (HS) has been indicated to increase ruminal temperature, increase digestibility, and reduce ruminal pH of steers fed high concentrate diets. However, it is unclear if this effect is the result of greater fermentation rate, slower passage rate or a combination. The effect of ruminal incubation temperature on substrate digestibility and rate of fermentation were evaluated. Four cannulated British-crossbreed steers (BW = 520 kg ± 30 kg) consuming an 87% (DM) concentrate diet were utilized as rumen fluid donors in a randomized complete block design with two incubation treatmen

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Stotz, Miranda K., Sebastian E. Mejia-Turcios, Andrea M. Osorio, et al. "183 Effect of fermentation temperature on in vitro digestibility of a finishing diet." Journal of Animal Science 97, Supplement_1 (2019): 62–63. http://dx.doi.org/10.1093/jas/skz053.140.

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Abstract Heat stress (HS) has been indicated to increase ruminal temperature, increase digestibility, and reduce ruminal pH of steers fed high concentrate diets. However, it is unclear if this effect is the result of greater fermentation rate, slower passage rate, or a combination. The effect of ruminal incubation temperature on substrate digestibility and rate of fermentation were evaluated. Four cannulated British-crossbreed steers (BW = 520 kg ± 30 kg) consuming an 87% (DM) concentrate diet were utilized as rumen fluid donors in a randomized complete block design with two incubation treatme

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CHIQUETTE, J., K. J. CHENG, J. W. COSTERTON, and L. P. MILLIGAN. "EFFECT OF TANNINS ON THE DIGESTIBILITY OF TWO ISOSYNTHETIC STRAINS OF BIRDSFOOT TREFOIL (Lotus corniculatus L.) USING IN VITRO AND IN SACCO TECHNIQUES." Canadian Journal of Animal Science 68, no. 3 (1988): 751–60. http://dx.doi.org/10.4141/cjas88-084.

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Two new isosynthetic strains of birdsfoot trefoil (Lotus corniculatus L.), containing low or high concentrations of tannin, were tested using in vitro and in sacco digestibility techniques. The high tannin (HT) strain contained an average of five times more tannin on a dry matter basis, than the low tannin (LT) strain. After 24-h in vitro digestion, by a rumen inoculum, there was less (P < 0.01) dry matter disappearance (45.7 vs. 54.4%) from the HT strain. Also, the development of gas pressure was lower (P < 0.01) and production of volatile fatty acids less (P < 0.05) with the HT stra

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Suriyapha, Chaichana, Anusorn Cherdthong, Chanon Suntara, and Sineenart Polyorach. "Utilization of Yeast Waste Fermented Citric Waste as a Protein Source to Replace Soybean Meal and Various Roughage to Concentrate Ratios on In Vitro Rumen Fermentation, Gas Kinetic, and Feed Digestion." Fermentation 7, no. 3 (2021): 120. http://dx.doi.org/10.3390/fermentation7030120.

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The objective of this study was to determine the application of citric waste fermented yeast waste (CWYW) obtained from an agro-industrial by-product as a protein source to replace soybean meal (SBM) in a concentrate diet. We also determined the effect of various roughage to concentrate ratios (R:C) on the gas production kinetics, ruminal characteristics, and in vitro digestibility using an in vitro gas production technique. The experiment design was a 3 × 5 factorial design arranged in a completely randomized design (CRD), with three replicates. There were three R:C ratios (60:40, 50:50, and

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Barbi, J. H. T., E. Owen, and M. K. Theodorou. "To test the repeatability of a gas production method for assessing the in vitro fermentation of forages using micro-organisms derived from a rumen simulation technique (rusitec) or from sheep rumen liquor." Proceedings of the British Society of Animal Science 1995 (March 1995): 116. http://dx.doi.org/10.1017/s0308229600028828.

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When forage in vitro digestibilities are determined on different occasions, they can be different and this is generally related to differences in rumen liquor from which the microbial inoculum was obtained. Experiment 1 of the present study, using rumen liquor only, investigated whether fermentation kinetics of forages as determined by in vitro gas production assays (Theodorou et al, 1994) made on different occasions are repeatable. It was argued that kinetics of fermentation were likely to be more sensitive to quality of rumen micro-organisms than digestibility assessment.In previous experime

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Sun, Ling, Mingyung Lee, Seoyoung Jeon, and Seongwon Seo. "Evaluation of the Associative Effects of Rice Straw with Timothy Hay and Corn Grain Using an In Vitro Ruminal Gas Production Technique." Animals 10, no. 2 (2020): 325. http://dx.doi.org/10.3390/ani10020325.

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The objective of this study was to evaluate the associative effects of rice straw with timothy hay and corn grain. Using an automated gas production system, in vitro ruminal fermentation was studied for six substrates: 100% rice straw, 100% timothy hay, 100% corn grain, 50% rice straw and 50% timothy hay, 50% rice straw and 50% corn grain, and 50% rice straw, 25% timothy hay, and 25% corn grain. Incubation was performed in three batches with different rumen fluids to assess the in vitro ruminal gas production kinetics and rumen parameters (pH, NH3-N, volatile fatty acid (VFA), and true dry mat

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Magaña-Sevilla, H., CA Sandoval-Castro, and C. Capetillo-Leal. "Evaluation of a fat protection method (Ca-soap) with an in vitro gas production system." BSAP Occasional Publication 34 (2006): 125–29. http://dx.doi.org/10.1017/s1463981500042369.

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SummaryAn in vitro gas production technique was used to evaluate a fat protection method (Ca-soap). The Ca-soaps were prepared by incorporation of oil into a slaked lime solution which had been prepared immediately before. The proportions employed were 10:1 (oil:slaked lime). A feed sample of 0.5g was incubated in a N-rich medium, using 6 ml ruminal liquor and 54 ml medium. A factorial design 2x3 (2 inoculum sources from cows fed with and without Ca-soap x 3 diets) was employed. Diets were A) Control: 70% grass (Pennisetum purpureum var. Taiwan), 30% sorghum grain; B) Soybean lecithin: 70% gra

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