Relevant bibliographies by topics / In vitro vegetative propagation

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Academic literature on the topic 'In vitro vegetative propagation'

Author: Grafiati
Published: 4 June 2021
Last updated: 17 February 2022

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Journal articles on the topic "In vitro vegetative propagation"

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Dubchak, Marcela, Olga Sultanova, and Viktor Bondarchuk. "Propagation of phytosanitary clones by in vitro culture." BIO Web of Conferences 34 (2021): 03003. http://dx.doi.org/10.1051/bioconf/20213403003.

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This article presents the process of accelerated reproduction of healthy clones of grapes, including the following stages: growing young shoots of the original plants of clones, introducing tops into in vitro culture, microclonal cuttings, adaptation of microplants to ex vitro culture, transplanting into cassettes with a soil substrate, transferring plants to a greenhouse for growing to the condition of vegetative seedlings and planting in a pre-propagation mother stock. For the successful implementation of each of the above operations in the SPIHVFT, a Cultural Complex has been equipped, consisting of a number of interconnected premises: a sterile box, a culture chamber and a vegetation chamber. The use of this Complex allows multiplying the required number of plants during the year, to grow vegetative seedlings by the spring of the next year and plant them in the pre-propagation “Pre-base” mother plant. After a year, grafted vegetative seedlings grown from the vines of the mother plant were used for laying the mother stock.
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Pierik, R. L. M., A. van Voorst, G. Booy, C. A. M. van Acker, C. L. C. Lelivelt, and J. C. de Wit. "* VEGETATIVE PROPAGATION OF ALSTROEMERIA HYBRIDS IN VITRO." Acta Horticulturae, no. 226 (June 1988): 81–90. http://dx.doi.org/10.17660/actahortic.1988.226.7.

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Deng, S. Y., I. M. Heap, and T. A. Klein. "In vitro vegetative propagation of Chinese cabbage." Plant Cell, Tissue and Organ Culture 26, no. 2 (August 1991): 135–39. http://dx.doi.org/10.1007/bf00036117.

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Pierik, R. L. M., H. H. M. Steegmans, A. A. Elias, O. T. J. Stiekema, and A. J. van der Velde. "* VEGETATIVE PROPAGATION OF SYRINGA VULGARIS L. IN VITRO." Acta Horticulturae, no. 226 (June 1988): 195–204. http://dx.doi.org/10.17660/actahortic.1988.226.22.

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Kanwar J, K., and S. Kumar. "In vitro propagation of Gerbera: A Review." Horticultural Science 35, No. 1 (February 12, 2008): 35–44. http://dx.doi.org/10.17221/651-hortsci.

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Gerbera has gained popularity in the past few years in many countries of the world and it is in great demand in the floral industry as cut flower as well as potted plant due to its beauty, colour, long vase life, and ability to rehydrate after long transportation. The most commercial cultivars are propagated through vegetative means by multiplication through divisions of clumps; however, the multiplication by this method is too slow to be commercially viable. To commercialize this crop and to meet the growing demand for planting material, tissue and organ culture techniques are being used as alternative methods for propagation in many countries. Most of the work has been carried on plant regeneration by adventitious organogenesis from capitulum, shoot tip, leaf, petiole and other parts of the plant. Attention should be paid to improve the technology to achieve 100% success in all species/cultivars to meet growing demands of the growers globally. From the literature, it is evident that gerberas are highly amenable to in vitro studies, as various explants were found to favourably respond to different culture media with different types and concentrations of growth regulators.
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Chalupa, V. "In vitro propagation of European larch (Larix decidua Mill.)." Journal of Forest Science 50, No. 12 (January 11, 2012): 553–58. http://dx.doi.org/10.17221/4656-jfs.

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Methods of in vitro propagation of Larix decidua are described in the paper. The influence of explant source, tree age, genotype, composition of nutrient media and phytohormones on in vitro propagation of Larix decidua has been investigated. Needles, isolated vegetative buds, shoot tips, zygotic embryos and cotyledons were used as initial explants. Axillary and adventitious bud cultures were used for fast in vitro shoot multiplication. Root induction was stimulated on low salt medium supplemented with auxin. After rooting and hardening off, micropropagated trees were planted in experimental plots. The growth of micropropagated trees was comparable with the height and diameter growth of trees originated from seeds.
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Dhawan, Vibha, and Sant S. Bhojwani. "In vitro vegetative propagation of Leucaena leucocephala (Lam.) de Wit." Plant Cell Reports 4, no. 6 (December 1985): 315–18. http://dx.doi.org/10.1007/bf00269887.

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Agbohessou, M., K. V. Salako, R. Idohou, R. C. Gbedomon, A. Hounkpèvi, F. J. Chadare, R. Glèlè Kakaï, and A. E. Assogbadjo. "Status of vegetative propagation of baobab: A review." African Crop Science Journal 28, s1 (October 2, 2020): 215–24. http://dx.doi.org/10.4314/acsj.v28i1.16s.

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The African baobab (Adansonia digitata L.) is a large tree of great socio-economic and cultural importance in Africa, with almost all the parts of the species used for various purposes. A major concern about baobab fruit pulp production is the long time it takes for first fruiting (about 15 years). Vegetative propagation offers several advantages with regard to consumers’ preferences and precociousness of fructification. The objective of this study was to synthesise existent knowledge related to vegetative propagation methods of baobab and examine future prospects for improving the species propagation. This will ultimately contribute to better integrate baobab-based agroforestry systems into the diversification and poverty alleviation programmes. It is clear that cutting, grafting and in vitro multiplication are the vegetative propagation methods already tested on baobab. The success of grafting methods ranges from 10 to 89%, depending on the technique used. The Murashige and Skoog environment, supplemented with or without growth regulator hormones is by far the best condition for the in vitro reactivity of baobab explants, regardless of their types. With regards to cuttings, the average success rates stand around 30% when Indole-3-butyric acid (IBA) hormone is used. Other approaches such as marcotting techniques are yet to be tested and data on fruit production using these techniques are still needed in order to determine the best promising method for rapid and efficient vegetative propagation of baobab.
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Pinto, José E. B. P., Clovis M. Souza, and W. R. Maluf. "In Vivo and in Vitro Propagation of Selected Cabbage (Brassica oleracea L. var. capitata) Clones." HortScience 31, no. 4 (August 1996): 628d—628. http://dx.doi.org/10.21273/hortsci.31.4.628d.

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Hybrid cabbage cultivars can be produced via seed-propagated self-incompatible (SI) inbred lines, or, alternatively, via vegetative propagation of SI clones. Cabbage clones differ in their ability to undergo vegetative propagation, a fact that appears to be related to the degree of differentiation of the axillary buds inside the head. A procedure for in vivo and in vitro propagation is described for cabbage clones known for difficulty in undergoing vegetative propagation. Cuttings from clonal families 800 (easy-to-propagate) and 007 (difficult to propagate) were immersed in indolebutyric acid (IBA—0, 5, 25, and 125 mg·L–1) + boric acid (100 mg·L–1) + sucrose (20 g·L–1) for 15 hours and maintained in glasshouses. Induction of roots was more effective with 125 mg·L–1 IBA supplemented with boric acid and sucrose. This treatment showed the highest frequency of rooting and the largest number of roots per cutting. The in vitro system of propagation was performed on the basal medium of Murashige and Skoog (MS), to which triadizuron (TDZ), benzyladeninepurine (BAP), and kinetin (Kin) were added in different combinations. TDZ was more effective than BAP or Kin in the promotion of shoot regeneration.
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Galopin, Gilles, François Beaujard, and Michel Gendraud. "Intensive production of juvenile cuttings by mother microplant culture in Hydrangea macrophylla "Leuchtfeuer"." Canadian Journal of Botany 74, no. 4 (April 1, 1996): 561–67. http://dx.doi.org/10.1139/b96-071.

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The vegetative propagation of hydrangea (Hydrangea macrophylla "Leuchtfeuer") should be explored through the architectural concepts of woody plants. The formation and continued culture of mother microplants in the greenhouse with high relative humidity and long days make up the new propagation method. Vegetative growth is characterized by the prolific production of homogenous cuttings as basal shoots in which the morphology of the axis is similar to that of plants produced by germination. There is considerable capacity for high levels of production, and it is a possible alternative to in vitro and traditional propagation. The morphogenetic basis of the functioning of these cultures is discussed in relation to juvenility and differentiation in woody plants. Keywords: morphogenesis, Hydrangea macrophylla, vegetative propagation, juvenility.
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Dissertations / Theses on the topic "In vitro vegetative propagation"

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Lucho, Simone Ribeiro. "PROPAGAÇÃO IN VITRO E EX VITRO DE Symplocos uniflora (POHL.) BENTH. (SYMPLOCACEAE)." Universidade Federal de Santa Maria, 2014. http://repositorio.ufsm.br/handle/1/4871.

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The Symplocos uniflora species is a native tree that reaches up to 10m tall, belonging to the Symplocaceae family. It is popularly known as pau-de-canga, maria-mole-do-banhado and sete-sangria. Virtually, there is no information in the literature on reproduction, growth and development of this species, whose studies of forms of propagation is essential. Thus, the present study intended to examine the sexual and vegetative propagation of the species S. uniflora through protocols for seed germination and micropropagation. Fruit and vegetative material of adult plants from the Botanical Garden of UFSM were collected. The fruits without pulp, containing the seeds were used to evaluate the effect of temperature, storage time, light, collection time, the application of chemical and mechanical scarification, besides the use of gibberellic acid on seed germination of S. uniflora. Plants grown in a greenhouse were used as donor explants (nodal and apical segment) in order to obtain the direct organogenesis. In the study, MS medium and different combinations of auxin naphthaleneacetic acid (NAA) and cytokinin 6-benzylaminopurine (BAP) were used. The presence of light is essential for the germination of Symplocos uniflora, being classified as positive photoblastic preferred. The use of pre-germination treatment with concentrated sulfuric acid is favorable for overcoming seed dormancy. Of the substrates analyzed, the filter paper showed the best germination percentages and speed of germination. Plantmax® substrate offered the highest length of air shoots. In micropropagation, the average percentage of air shoots was 29% with no occurrence of root formation.
Symplocos uniflora (Symplocaceae) é uma árvore que atinge até 10 m de altura, nativa e conhecida popularmente como pau-de-canga, maria-mole-do-banhado e sete-sangria. O trabalho visou estudar a propagação sexuada e vegetativa da espécie, através de protocolos para a germinação de sementes e micropropagação. Endocarpos concrescidos com as sementes (diásporos) foram utilizados para estudos da germinação das sementes avaliando temperatura de armazenamento (10 e 25ºC), temperaturas de incubação (15, 20, 25 e 30ºC), regimes de luz (fotoperíodo de 16 horas e escuro contínuo), época de coleta dos frutos (janeiro e março de 2013), escarificação química (ácido sulfúrico concentrado por 10 minutos), escarificação mecânica (desponte do endocarpo) e ácido giberélico (GA3), 1,5 m L-1. Plantas cultivadas em casa de vegetação, com 18 meses de idade, foram utilizadas como doadoras de explantes (segmento nodal e apical), visando à obtenção da organogênese direta. No estudo foi utilizado o meio MS e diferentes combinações (tratamentos) de ácido naftalenoacético (ANA) e 6-benzilaminopurina (BAP), em mg L-1: 0,0; 0,1; 0,2 e 0,4 e 0,0; 1,0; 2,0 e 4,0, respectivamente. A temperatura de 25ºC promoveu a maior porcentagem de germinação das sementes (24%). Frutos maduros apresentaram as melhores porcentagens de germinação (30%) e índice de velocidade de germinação (1,19). A presença de luz favoreceu à germinação das sementes, sendo classificadas como fotoblásticas positivas preferenciais. O tratamento pré-germinativo com ácido sulfúrico concentrado por 10 minutos promoveu a superação da dormência das sementes. Na germinação in vitro o uso de GA3 no meio de cultura propiciou os melhores índices de velocidade de germinação (0,18), não influenciando na porcentagem da mesma. No cultivo in vitro, a maior porcentagem de explantes com brotações aéreas (29%) ocorreu na combinação de 0,2 mg L-1 de ANA e 2,0 mg L-1 de BAP, sem ocorrência de formação de raízes. Os explantes utilizados e brotações obtidas in vitro apresentaram taxas de oxidação variando de 10 a 70%.
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Ferri, Juçara. "Micropropagação e desenvolvimento vegetativo de mirtilo." Universidade Federal de Pelotas, 2008. http://repositorio.ufpel.edu.br/handle/ri/2116.

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Made available in DSpace on 2014-08-20T14:22:05Z (GMT). No. of bitstreams: 1 dissertacao_jussara_ferri.pdf: 17890283 bytes, checksum: 29dfc3e77cedf523a06e365180e450bf (MD5) Previous issue date: 2008-03-27
Blueberry cultivation is recent in Brazil, and the area expansion of this production is limited by the lack of quality seedlings. The in vitro propagation make possible, in little time, to produce of mass form, high quality plants. In this work, the first objective was to study some involved factors in the establishment, multiplication and rooting of blueberry rabbiteye group, seeking yield increase of the seedlings production through micropropagation. The second objective was to study some involved factors seedlings development of the Aliceblue, Climax, O Neal and Georgiagem cultivars, seeking make available to producers seedlings sufficiently developed and adapted to orchard implantation. The work was developed in 2 stages. The first stage was constituted for 4 trials micropropagation related, and consisted respectively in the in vitro establishment, multiplication of the material previously in vitro established and rooting sprout obtained in the multiplication stage. Was verified that zeatin is necessary for in vitro establishment Powderblue and Florida cultivars, be able to utilize the smaller concentration (9 μM). Cultivars Woodard, Bluebelle and Bluegem explants presented the most bud number. The most rooting percentage was obtained with 6 µM of IBA added in culture media. The basal stakes presented the most root quantity. The 6 µM concentration of growth regulator ANA provide the most root length. The second stage of this work was constituted for 2 trials vegetative establishment related. In the first trial were utilized 2 blueberry cultivars of rabbiteye group and 4 substrates tips. In the second trial were utilized 2 cultivars blueberry group and 4 substrates tips. It recommended 70% sawdust + 20% coconut fiber + 10% bovine manure mixture to the most growth aerial part, the most mass of dry matter aerial and root, and the most sprout number. For the rabbiteye group, Climax cultivar presented the most vegetative development, parallel O´Neal cultivar highbush group.
O cultivo do mirtilo no Brasil é recente, e a expansão da área de produção é limitada pela disponibilidade de mudas de qualidade. A propagação in vitro possibilita em curto espaço de tempo, produzir de forma massal, plantas sadias com alta qualidade. Neste trabalho, o primeiro objetivo foi estudar alguns dos fatores envolvidos no estabelecimento, na multiplicação e no enraizamento de cultivares de mirtilo do grupo rabbiteye , visando o aumento do rendimento na produção de mudas através da micropropagação. O segundo objetivo foi estudar alguns dos fatores relacionados ao desenvolvimento de mudas das cultivares Aliceblue, Climax, O Neal e Georgiagem, visando disponibilizar ao produtor uma muda suficientemente desenvolvida e adaptada para a implantação de pomares. O trabalho foi realizado em duas etapas. A primeira etapa foi constituída por 4 experimentos relacionados a micropropagação e consistiram respectivamente em estabelecimento, multiplicação do material já estabelecido in vitro e enraizamento de brotações obtidas na fase de multiplicação. Foi verificado que zeatina é necessária para o estabelecimento in vitro das cultivares Powderblue e Florida, podendo ser utilizada a menor concentração (9 μM). Explantes das cultivares Woodard, Bluebelle e Bluegem na posição vertical do explante apresentaram maior número de gemas. A maior porcentagem de enraizamento na cv. Climax foi obtida com a adição de 6 µM de AIB no meio de cultura. As estacas basais apresentaram maior quantidade de raiz. O fitorregulador ANA a 6 µM, proporciona maior comprimento de raiz. A segunda etapa deste trabalho constituiu-se de dois experimentos relacionados ao desenvolvimento vegetativo. No primeiro foram utilizadas 2 cultivares de mirtilo do grupo rabbiteye e 4 tipos de substratos. No segundo experimento foram utilizadas 2 cultivares de mirtilo do grupo highbush e 4 tipos de substratos. Recomenda-se a mistura 70% serragem + 20% fibra de coco + 10% esterco bovino para maior crescimento da parte aérea, maior massa da matéria seca da parte aérea e do sistema radicular e maior número de brotações. Para o grupo rabbiteye a cultivar Climax foi a que apresentou maior desenvolvimento vegetativo, paralelamente à cultivar O´Neal para o grupo highbush'.
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Navroski, Marcio Carlos. "MULTIPLICAÇÃO in vitro DE GENÓTIPOS DE Eucalyptus dunnii MAIDEN." Universidade Federal de Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/8676.

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Eucalyptus is a genus of great importance to the economy and production of various goods. Among the species of this genus, Eucalyptus dunnii Maiden stands out the importance, especially for the states of southern Brazil, to present a good growth and resistance to frost. However, this species presents problems for the production of seedlings by cloning, mainly due to the difficulty of rooting. The objective of this research was to assess methodologies for surface disinfection, establishment, multiplication, elongation and rooting in vitro of selected genotypes of Eucalyptus dunnii. The genetic material used in this study were collected in areas of the company Stora Enso, in the county of Alegrete - RS in commercial stands of Eucalyptus dunnii about three years old, originated from the planting of seeds. After 60 days, harvested, shoots were collected from 10 selected genotypes, which were isolated from cuttings. In the laboratory, stem segments were isolated at three positions on the cuttings: top, middle and base portion, which were evaluated in procedures for surface disinfection with sodium hypochlorite. We tested different immersion times in disinfest 1.5% and in another experiment, different concentrations of immersion for 10 minutes. Subsequently, we evaluated the performance of genotypes in in vitro establishing. During multiplication were evaluated concentrations of the growth regulators 6-benzylaminopurine (BAP). At the elongation step there were two experiments, one using gibberellic acid (GA3) in varying concentrations in the presence of alpha-Naphthalene acetic acid (NAA) and BAP, and another using only NAA and BAP. In the rooting phase two experiments were conducted, using an IBA in nutrient medium and another pulse treatment with IBA and vermiculite in a liquid medium, the rooted cuttings were acclimatized in plastic cups. As a result it was possible to establish satisfactory six genotypes, and the phenol oxidation and contamination of the main causes of non-establishment of some genotypes. In the multiplication, BAP in a concentration range between 0.25 and 0.75 mg L-1 (1.11 and 3.33 μM), positively influences the formation of buds, there is a behavior varied depending on genotype. At the elongation is also genotypic influence and the use of NAA at 0.5 mg L -1 (2.69 μM) plus BAP promotes the greatest elongation of shoots. The use of GA3 in the nutritive medium of elongation may be waived, because don t promote elongation of the shoots and cause the formation of callus. AIB added to the ½ MS nutritive medium has no effect on in vitro rooting of Eucalyptus dunnii. for the use of vermiculite associated with ½ WPM liquid nutritive medium after treatment pulse with solution of IBA promote root induction in some cuttings and may be considered in further studies.
Eucalyptus é um gênero de grande importância para a economia e produção de vários bens. Entre as espécies deste gênero, Eucalyptus dunnii Maiden destaca-se pela importância, principalmente para os estados da região sul do Brasil, por apresentar um bom crescimento e resistência as geadas. Entretanto, essa espécie apresenta problemas em relação à produção de mudas por clonagem, devido, principalmente, à dificuldade de enraizamento. O objetivo geral desta pesquisa foi avaliar metodologias para a desinfestação superficial, o estabelecimento, a multiplicação, o alongamento e o enraizamento in vitro de genótipos selecionados de Eucalyptus dunnii. O material genético utilizado no presente estudo foi coletado em áreas da empresa Stora Enso, localizadas no município de Alegrete - RS, em povoamentos comerciais de Eucalyptus dunnii de cerca de três anos de idade, originados do plantio de sementes. Decorridos 60 dias do abate das árvores, foram coletadas brotações de 10 genótipos selecionados, das quais foram isoladas estacas. No Laboratório, foram isolados segmentos caulinares em três posições nas estacas: ápice, porção intermediária e base, os quais foram avaliados em procedimentos de desinfestação superficial com hipoclorito de sódio. Foram testados diferentes tempos de imersão neste desinfestante a 1,5% e, em outro experimento, diferentes concentrações na imersão durante 10 minutos. Posteriormente, foi avaliado o desempenho dos genótipos no estabelecimento in vitro. Na etapa de multiplicação foram avaliadas concentrações do regulador de crescimento 6-Benzilaminopurina (BAP). Na etapa de alongamento realizaram-se dois experimentos, um utilizando ácido giberélico (GA3) em concentrações variáveis na presença de Ácido alfa-Naftaleno Acético (ANA) e BAP, e outro utilizando apenas ANA e BAP. Na fase de enraizamento foram realizados dois experimentos, um utilizando AIB no meio nutritivo e outro, com tratamento pulso de AIB e, após, plantio em copos plásticos contendo vermiculita e meio líquido, para aclimatização das estacas em sala de cultivo. Foi possível o estabelecimento in vitro satisfatório de seis genótipos, sendo a oxidação fenólica e a contaminação as principais causas do fracasso de alguns genótipos. Na multiplicação, BAP, na faixa de concentração entre 0,25 e 0,75 mg L-1 (1,11 e 3,33 μM), influencia positivamente a formação de gemas por explante, ocorrendo um comportamento variado no comprimento e número de gemas por explante conforme o genótipo. No alongamento há também influência genotípica e a utilização de ANA na concentração de 0,5 mg L -1 (2,69 μM) associada a BAP promove o maior alongamento das brotações. A utilização de GA3 no meio de alongamento pode ser dispensada, pois além de não promover o alongamento das brotações causa a formação de calos. AIB adicionado ao meio nutritivo ½ MS não tem efeito no enraizamento in vitro de microestacas de Eucalyptus dunnii. O emprego de vermiculita associado a meio nutritivo ½WPM líquido, após tratamento pulso com solução de AIB promove a indução de raiz em algumas estacas, podendo ser considerado em estudos adicionais.
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Heberle, Michele. "PROPAGAÇÃO IN VITRO E EX VITRO DE LOURO-PARDO (Cordia trichotoma (Vell.) Arrabida ex Steudel)." Universidade Federal de Santa Maria, 2010. http://repositorio.ufsm.br/handle/1/8665.

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Although the louro-pardo (Cordia trichotoma Vell.) is native forest specie with high timber potential, there are still scarce studies that approach the production of seedlings of this species by vegetative propagation. The objective of this work was to evaluate the vegetative propagation of the louro-pardo by the techniques of cuttings and micropropagation. It was tested the presence or absence of 8000 mg L-1 of indolbutiric acid (IBA) and two types of cuttings (basal and apical). At 40 days, the cuttings were evaluated for survival, rooting, the presence of callus and shoots, the number and length of shoots and the number of leaves. For the establishment of aseptic seedlings, seeds of louro-pardo were treated with 2% or 5% of sodium hypochlorite, for 0, 5, 10, 15 or 20 min. The seeds were inoculated in WPM basic culture medium. At 30 days, the percentages of disinfection, fungal contamination and / or bacteria and the average time of germination were evaluated. For multiplication, apical segments were inoculated in basic medium, plus 0; 0,25; 0,50 or 0,75 mg L-1 of 6-benzilaminopurin (BAP). At 45 days were evaluated the length, number of leaves and internodes of the shoots, the percentage of callus and the survival of the explants. In the multiplication, using microstumps kept in vitro, was tested whether or not the addition of 1,5 g L-1 of activated charcoal to the basic medium on the production of shoots. At 30 and 60 days, concerning to the first and second cuts of microstumps, were evaluated the number and length of shoots, number of leaves and internodes of the shoots, and the number of microcutting. Rooting in vitro microcuttings were maintained in basic medium plus 1,5 g L-1 of activated charcoal and IBA (1,5 or 2,0 mg L-1). At 45 days were evaluated the presence of roots and callus, the percentage of survival and sprouting. In cutting, were observed the formation of shoots on the cuttings, but they are not rooted. The cutting type and dose of IBA did not influence the rooting and survival. In micropropagation was found that the sterilization of seeds with 5% of sodium hypochlorite for 5 min. allowed the in vitro establishment of aseptic seedlings. The induction of shoots in the explants was not influenced by different doses of BAP. The presence of activated charcoal in the culture medium favored the formation and growth of shoots in microstumps. The use of 1,5 or 2,0 mg L-1 IBA did not promote the rooting of microshoots of louro-pardo.
Apesar de o louro-pardo (Cordia trichotoma Vell.) ser uma espécie florestal nativa com elevado potencial madeireiro, ainda são escassos os estudos que abordam a produção de mudas dessa espécie pela propagação vegetativa. O objetivo deste trabalho foi avaliar a propagação do louro-pardo pelas técnicas de estaquia e micropropagação. Para a estaquia foi testada a presença ou ausência de 8000 mg L-1de ácido indolbutírico (AIB) e dois tipos de estacas (basais e apicais). Aos 40 dias, as estacas foram avaliadas quanto à sobrevivência, o enraizamento, a presença de calos e de brotos, o número e o comprimento de brotos e o número de folhas. Para o estabelecimento de plântulas assépticas, sementes de louro-pardo foram tratadas com 2% ou 5% de hipoclorito de sódio, por 0, 5, 10, 15 ou 20 min. As sementes foram inoculadas em meio de cultura base WPM. Aos 30 dias foram avaliadas as porcentagens de desinfestação, de contaminação por fungos e/ou bactérias e o tempo médio de germinação. Para a multiplicação, ápices caulinares foram inoculados em meio de cultura base, acrescido de 0; 0,25; 0,50 ou 0,75 mg L-1 de 6-benzilaminopurina (BAP). Aos 45 dias avaliou-se o comprimento, o número de folhas e de entrenós dos brotos, as porcentagens de calo e de sobrevivência dos explantes. Na multiplicação, utilizando microcepas mantidas in vitro, testou-se a adição ou não de 1,5 g L-1 de carvão ativado ao meio de cultura base na produção de brotos. Aos 30 e 60 dias, referentes ao primeiro e ao segundo corte das microcepas, foram avaliados o número e o comprimento dos brotos, o número de folhas e de entrenós dos brotos, e o número de microestacas. No enraizamento in vitro, microestacas foram mantidas em meio de cultura base, acrescido de 1,5 g L-1 de carvão ativado e AIB (1,5 ou 2,0 mg L-1). Aos 45 dias foram avaliadas a presença de raízes e calos, a porcentagem de sobrevivência e de brotação. Na estaquia, foi observada a formação de brotos nas estacas, contudo estas não enraizaram. O tipo de estaca e a dose de AIB utilizada não influenciaram no enraizamento ou na sobrevivência. Na micropropagação foi verificado que a assepsia das sementes com 5% de hipoclorito de sódio, por 5 min., possibilitou o estabelecimento in vitro de plântulas assépticas. A indução de brotos nos explantes não foi influenciada pelas diferentes doses de BAP. A presença de carvão ativado no meio de cultura base favoreceu a formação e o crescimento dos brotos nas microcepas. A utilização de 1,5 ou 2,0 mg L-1 de AIB não promoveu o enraizamento adventício das microestacas de louro-pardo.
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Horbach, Micheli Angelica. "PROPAGAÇÃO IN VITRO E EX VITRO de erva-mate (Ilex paraguariensis Saint Hilaire Aquifoliaceae)." Universidade Federal de Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/8628.

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Mate (Ilex paraguariensis) is an important species in Southern Brazil for its economical and cultural values. The present work had the objectives of analyzing the propagation of mate by cuttings, disinfection and in vitro establishment of nodal segments from adult plants and micropropagation of plants originated from immature embryos. Mate cutting were submitted to three (0, 4000 and 8000 mg L-1) IBA doses and two lengths (3 and 10 cm) of cutting. The cuttings were evaluated at 135 days for survival, rooting and formation of roots, leaves and shoots. In the disinfection experiments, nodal segments of one-year-old shoots were immersed in alcohol solution of 70% for two and four min., in 2% of NaOCl for 15, 25 and 35 min. and inoculated in ¼ of MS medium. The percentage of contaminated, oxidized and healthy explants were evaluated at 15 days. In micropropagation, mate seedlings from immature embryos were submitted to different culture mediums (MS, ¼ MS and WPM) for 30 days and evaluated for number of leaves and length of seedlings. For the multiplication, different doses (0; 0,01; 0,1; 1,0 and 2,0 mg L-1) of BAP were tested in ¼ MS culture medium. At 50 days, they were evaluated for the number of shoots, leaves and internodes, shoots length and height. Different doses (0; 0,5; 1,0 and 1,5 mg L-1) of IBA were evaluated for rooting and kept explants either for 15 or 30 days in the medium. In vitro plants of mate were acclimatized in different (vermiculite, sand or coconut shells) substrates, with controlled temperature and light environmental conditions. The highest percentage of rooted cuttings, in both evaluated length, were with 4000 mg L-1 of IBA. Cuttings with 3 cm length produced the highest percentage of rooted cuttings. Nodal segments of one-year-old shoots have the highest survival, with the disinfection with alcohol 70% for four minutes and NaOCl 2% for 25 min. The ¼ of MS salts medium can be used as basal medium for in vitro culture of mate plants. The basal medium with 2 mg L-1 of BAP increases the number of adventitious shoots and promotes shoot proliferation after some sub cultivations, enabling in vitro plant maintenance as microstump, as shoot supply. In vitro rooting can be achieved in one cycle of 30 days of cultivation, with the addition of 3 mg L-1 of IBA to the medium with ¼ of MS salts. In vitro plants of mate can be acclimatized in coconut shells or sand in controlled temperature and light conditions.
A erva-mate (Ilex paraguariensis) é uma espécie de grande importância no sul do Brasil, pelo valor econômico e cultural que apresenta. O presente trabalho teve por objetivos analisar a propagação de erva-mate por estaquia, o estabelecimento e desinfestação in vitro de segmentos nodais de plantas adultas e a micropropagação de plantas oriundas de embriões imaturos. Para a estaquia, testaram-se três doses de AIB (0, 4000 e 8000 mg L-1) e dois comprimentos de estacas (3 e 10 cm). As estacas foram avaliadas quanto à sobrevivência, o enraizamento, o número de raízes, de folhas e de brotos e o comprimento de raízes e de brotos, aos 135 dias. No experimento de desinfestação, segmentos nodais de brotações de ano de erva-mate foram imersos em etanol 70%, por 2 e 4 min. e, depois, em NaOCl 2%, por 15, 25 e 35 min e, então inoculados em meio com ¼ do MS. Aos 15 dias avaliaram-se a percentagem de explantes contaminados, oxidados e sadios. Na micropropagação, plântulas de erva-mate, obtidas de embriões imaturos, foram submetidas a diferentes meios de cultura (MS, ¼ MS e WPM) por 50 dias, com avaliação do incremento obtido para as variáveis número de folhas e comprimento de plântulas. Para a multiplicação dessas plantas, foram testadas a adição de diferentes doses (0; 0,01; 0,1; 1,0 e 2,0 mg L-1) de BAP ao meio de cultura com ¼ do MS. Foram avaliados o número de brotos, folhas e entrenós, o comprimento dos brotos e a altura das plantas, aos 50 dias de cultivo. No enraizamento de brotos de erva-mate foram utilizadas doses (0; 0,5; 1,0; 1,5; 3,0 e 6,0 mg L-1) de AIB, por 15 e 30 dias. As avaliações constaram da percentagem de enraizamento e de calo obtidas, do número e comprimento de raízes, do número de folhas e altura das plantas, aos 30 dias de cultivo. Plantas de erva-mate, produzidas in vitro, foram aclimatizadas em diferentes substratos (vermiculita média, areia e casca de coco) com temperatura e luminosidade controladas. A maior percentagem de estacas enraizadas, em ambos os comprimentos, foi com a dose de 4000 mg L-1 de AIB, sendo que as estacas de 3 cm obtiveram uma maior percentagem de enraizamento. Segmentos nodais de brotações de ano de erva-mate apresentam a maior sobrevivência com a desinfestação com álcool 70% por 4 min. e em NaOCl 2% por 25 min. O meio com ¼ dos sais do MS pode ser utilizado como o meio de cultura base para o cultivo in vitro de plantas de erva-mate. A adição de 2 mg L-1 de BAP ao meio de cultura base aumenta o número de brotos adventícios em erva-mate, além de formar tufos após alguns subcultivos, possibilitando a manutenção de plantas como microcepas, para o fornecimento de brotos. O enraizamento in vitro de erva-mate pode ser realizado em apenas uma fase de 30 dias, com a adição de até 3 mg L-1 de AIB ao meio com ¼ dos sais do MS. Plantas de erva-mate, produzidas in vitro, podem ser aclimatizadas nos substratos casca de coco ou areia em ambiente com temperatura e luminosidade controladas.
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León, Enrique Asterio Benítez. "QUALIDADE DE SEMENTES, MICROPROPAGAÇÃO, CONSERVAÇÃO IN VITRO E ISOLAMENTO DE DNA GENÔMICO DE Luehea divaricata Mart. & Zucc." Universidade Federal de Santa Maria, 2014. http://repositorio.ufsm.br/handle/1/3768.

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Luehea divaricata Mart. & Zucc., is a native species, found in the Atlantic Forest and Pampa biomes in Rio Grande do Sul state, Brazil, belonging to the Malvaceae family. Considering the need for recovery and restoration of degraded areas, the demand for seeds and seedlings of native species may register significant increases, and the contribution of research studies to native species will be of great importance for both the forestry and the environmental sectors. This study aimed to research the quality of seeds, the development of methodologies for micropropagation and in vitro conservation of Luehea divaricata and isolation of genomic DNA of the species. Physiological and sanitary quality tests of seeds stored in Kraft paper inside refrigerator, in two different periods, collected from different places of its natural habitat, were conducted. The development of methodologies for micropropagation studies were divided in different experiments, testing at first, the response of cultures initiated from epicotyl, to stimuli from different sources and concentrations of cytokinin on in vitro multiplication. Afterwards, the pulse effect treatment with concentrations of IBA on the rooting ability of in vitro nodal segments cultivated in nutrient medium MS or WPM were tested both presence or absence of activated charcoal. For acclimatization of micropropagated plants, the effect of different concentrations of sucrose in the nutrient medium WPM and two kinds of commercial substrates were evaluated. The effect of the, osmotic regulator, mannitol, presence or absence sucrose, on the performance of in vitro nodal segments, aimed at in vitro conservation of germplasm was also observed. Three protocols were tested to isolate genomic DNA from young leaves, proving its efficiency and quality by spectrophotometry quantification and digestion with the Hind III restriction endonuclease. The main results show that genotypes differ both in their physiological and sanitary quality, and maintain their viability after six months of storage in a refrigerator. For in vitro multiplication of nodal segments, the addition of cytokinins to the nutrient medium is dispensable ,and when added, promote callus formation, unwanted at such stage. The rooting of nodal segments is successful in nutrient medium WPM in the absence of activated charcoal and it was found that the pulse treatment with IBA concentrations exceeding 9μM may optimize the process. The concentration of 30 g L-1 sucrose in the nutrient medium WPM is effective in develop plants capable of acclimatization using either commercial substrate Mecplant® or H-Decker®. It is possible in vitro conservation of nodal segments under minimal growth for a period of 120 days with the use of mannitol combined with sucrose concentrations of up to 15 g L-1 in the nutrient medium ½MS. The DNA isolated protocols were effective to obtain DNA concentration and quality still with the presence of proteins and polysaccharides. The results may contribute to the existing information on the in vitro propagation of Luehea divaricata, supporting breeding programs, germplasm conservation and analysis of genetic variability in natural populations.
Luehea divaricata Mart. & Zucc., é uma espécie nativa, dos Biomas Mata Atlântica e Pampa, pertence à família Malvaceae, conhecida comumente como açoita-cavalo. Considerando-se a necessidade de recuperação e restauração de áreas degradadas, a demanda crescente por sementes e mudas de espécies florestais nativas, e a contribuição de trabalhos de pesquisa com espécies nativas será de grande importância para o setor florestal e ambiental, o presente trabalho objetivou avaliar a qualidade de sementes, analisar e selecionar metodologias para a micropropagação e para a conservação in vitro de Luehea divaricata e, também, para o isolamento de DNA genômico da espécie. Foram conduzidos testes de qualidade fisiológica e sanitária de sementes conservadas em papel Kraft no interior de refrigerador, em dois períodos distintos, coletadas em diferentes pontos de ocorrência natural da espécie. No desenvolvimento de metodologias para micropropagação os estudos foram divididos em diferentes experimentos, testando-se, primeiramente, as respostas de culturas iniciadas a partir de epicótilos, aos estímulos de diferentes fontes e concentrações de citocinina na multiplicação in vitro. Na sequência, foi avaliado o efeito do tratamento pulse com concentrações de AIB sobre a capacidade de enraizamento in vitro de segmentos nodais, cultivados em meios nutritivos MS ou WPM, na presença ou ausência de carvão ativado. Na fase de aclimatização de plantas micropropagadas foram avaliados o efeito de diferentes concentrações de sacarose no meio nutritivo WPM, e, também, de dois tipos de substratos comerciais. Foi verificado adicionalmente, o efeito do regulador osmótico manitol, associado ou não à sacarose, sobre o desempenho in vitro de segmentos nodais, visando à conservação in vitro de germoplasma. Foram testados três protocolos para isolar DNA genômico a partir de folhas jovens, comprovou-se sua qualidade e eficiência, por meio de quantificação por espectrofotometria e digestão com a endonuclease de restrição Hind III. Os principais resultados obtidos demostraram que sementes de açoita-cavalo apresentam diferenças na sua qualidade fisiológica e sanitária, e mantêm a sua viabilidade após seis meses de armazenamento em refrigerador. Para a multiplicação in vitro de segmentos nodais de açoita-cavalo a adição de citocininas ao meio nutritivo e dispensável, e quando adicionadas, promovem formação de calos, indesejados nessa fase. O enraizamento in vitro de segmentos nodais é bem-sucedido em meio nutritivo WPM na ausência de carvão ativado e obteve-se que o tratamento pulse com concentrações de AIB superiores a 9μM pode otimizar o processo. A concentração 30 g L-1 de sacarose no meio nutritivo WPM é eficiente na formação de plantas passíveis de aclimatização utilizando-se substrato comercial Mecplant® ou H-Decker®. É possível a conservação in vitro de segmentos nodais de açoita-cavalo, sob condições de crescimento mínimo, pelo período de 120 dias com a utilização de manitol combinado a concentrações de sacarose de até 15 g L-1, no meio nutritivo ½MS. Os protocolos de isolamento de DNA são eficientes em obter DNA com quantidade e qualidade aceitáveis, porém, ainda com a presença de proteínas e polissacarídeos. Os resultados obtidos poderão contribuir com as informações existentes sobre a propagação in vitro de açoita-cavalo, apoiando programas de melhoramento, conservação de germoplasma e análise da variabilidade genética de populações naturais.
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Godet, Xavier. "Biologie du colchique (colchicum autumnale l. ) : multiplication vegetative par voie traditionnelle et in vitro." Clermont-Ferrand 2, 1987. http://www.theses.fr/1987CLF2D189.

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Examen des cycles biologiques de la plante adulte et de la plante juvenile: les exigences thermiques, les potentialites du bourgeon vegetatif accessoire, variabilite individuelle pour la production de graines et d'alcaloides. Une methode de multiplication vegetative est recherchee: en pepiniere ou in vitro, possibilite de fragmenter les cornus; caulogenese in vitro. La possibilite d'une biosynthese d'alcaloides par des cellules de colchique cultivees in vitro est evoquee
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8

Richez-Dumanois, Christine. "Multiplication vegetative et conservation in vitro d'un clone selectionne de chanvre a fibres (cannabis sativa l. ) : influence sur le developpement et la teneur en cannabinoides." Paris 6, 1987. http://www.theses.fr/1987PA066602.

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Le chanvre fait l'objet de nombreux travaux de selection portant sur la qualite de la fibre et le contenu en cannabinoides des plantes. Le but de cette etude est la mise au point pour un clone de chanvre a fibre, de methodes de multiplication vegetative et de conservation, adaptees a la selection varietable du chanvre. L'etude du bouturage horticole dans differentes conditions thermiques et photoperiodiques a montre l'effet positif de l'alternance des temperatures sur le developpement morphologique et chimique des boutures ainsi que la necessite d'un eclairement continu pour le maintien des plantes-meres vegetatives. Cette recherche a revele l'existence de gradients chimiques et morphologiques generalement favorables au choix de la moitie superieure des plantes
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CHOUFFOT, STRUYCKEN BARBARA. "Contribution a l'etude des capacites de multiplication vegetative du shorea curtisii dyer ex king, diphterocarpaceae de malaisie." Strasbourg 1, 1986. http://www.theses.fr/1986STR13138.

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Surexploitee pour son bois, infeodee pour son developpement a une mycorhization, dependant d'une production de semences episodique et d'une germination aleatoire, shorea curtisii, incapable en outre de multiplication vegetative spontanee ou par les procedes traditionnels, est tres representatif des dipterocarpacees asiatiques menacees d'extinction rapide. Une mise au point d'un procede de micropropagation in vitro contribuerait a sauvegarder l'espece. L'observation, chez shorea curtisii, de galles epiphylles a organisation de bourgeon abortif suggerant une certaine aptitude des feuilles a la neoformation de meristemes caulinaires, privilegie celles-ci pour larecherche d'une methode de clonage in vitro (. . . )
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10

Beldjabri, Nacéra. "Culture in vitro chez diverses plantes florales (Lisianthus, pétunia, tabac et pervenche de Madagascar) dans le but d'embryogenèse somatique : obtention de cals et de régénérations." Vandoeuvre-les-Nancy, INPL, 1997. http://www.theses.fr/1997INPL116N.

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Dans ce travail, l'embryogenèse somatique a été tentée chez différentes espèces florales : le Lisianthus (Eustoma grandiflorum) le pétunia (Petunia hybrida), le tabac (Nicotiana glutinosa et Nicotiana langsdorfii) et la pervenche de Madagascar (Catharanthus roseus). Les expérimentations ont consisté en la culture in vitro de différents organes : anthères, ovaires, fragments de feuilles, fragments d'entre-nœuds et de pédoncules floraux de chacune des espèces étudiées. Les essais réalisés ont porté sur l'influence du prétraitement au froid, de la composition du milieu de culture, de la taille des boutons à mettre en culture et de la lumière et de l'obscurité. Outre l'obtention d'un embryon somatique issu d'une anthère de Lisianthus, des résultats intéressants et reproductibles de callogenèse et de régénération de plantes sont apparus à partir de tous les organes mis en culture chez le Lisianthus, le pétunia et le tabac. Les réponses des différentes espèces vis-à-vis des facteurs d'études en termes de callogenèse et de régénération, sont fonction de l'organe mis en culture et du stade de développement de chacun des éléments utilisés.
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Books on the topic "In vitro vegetative propagation"

1

Ansari, S. A. Vegetative propagation of trees. Jabalpur: Tropical Forest Research Institute, 1997.

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Pereira, Jomar da Paes. Seringueira: Formação de mudas, manejo e perspectivas no Noroeste do Paraná. Londrina, PR, [Brasil]: IAPAR, 1992.

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Mwila, Godfrey P. Eco-geographical studies and reviews of vegetatively propagated crop species occuring in Swaziland and Zambia. Lusaka: SADC Plant Genetic Resources Centre, 2008.

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Hvoslef-Eide, Anne Kathrine, and Walter Preil, eds. Liquid Culture Systems for in vitro Plant Propagation. Dordrecht: Springer Netherlands, 2005. http://dx.doi.org/10.1007/1-4020-3200-5.

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Jain, S. Mohan, and Sergio J. Ochatt, eds. Protocols for In Vitro Propagation of Ornamental Plants. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60327-114-1.

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Protocols for in vitro propagation of ornamental plants. New York: Humana Press, 2010.

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Smith, G. Spruce improvement through vegetative propagation: A story of co-operation. Ontario: Forest Resource Development Agreement, 1985.

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Dodds, John H. Transport, receipt, and propagation of in vitro potato plantlets. [Lima, Peru]: International Potato Center, 1986.

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Obdržálek, Jiří. Vegetativní množení listnatých dřevin. Průhonice: Výzkumný ústav okrasného zahradnictví, 1997.

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Gertsson, Ulla E. Development of a micropropagation method for Senecio x hybridus Hyl. and study of the progeny plants. Alnarp: Swedish University of Agricultural Sciences, Dept. of Horticultural Science, 1987.

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Book chapters on the topic "In vitro vegetative propagation"

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Pierik, R. L. M. "Vegetative propagation." In In Vitro Culture of Higher Plants, 183–230. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-1854-7_19.

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Pierik, R. L. M. "Vegetative propagation." In In Vitro Culture of Higher Plants, 183–230. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3621-8_20.

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Pierik, R. L. M. "Vegetative propagation." In In Vitro Culture of Higher Plants, 183–230. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5750-6_19.

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Pierik, R. L. M. "Vegetative propagation of orchids." In In Vitro Culture of Higher Plants, 159–67. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-1854-7_17.

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Pierik, R. L. M. "Vegetative propagation of orchids." In In Vitro Culture of Higher Plants, 159–67. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3621-8_18.

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Pierik, R. L. M. "Vegetative propagation of orchids." In In Vitro Culture of Higher Plants, 159–67. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5750-6_17.

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Bigot, C., and F. Engelmann. "Vegetative Propagation in Vitro of Cunninghamia Lanceolata (Lamb.) Hook." In Cell and Tissue Culture in Forestry, 114–27. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0992-7_9.

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Krähmer, Hansjörg. "Vegetative propagation." In Atlas of Weed Mapping, 371–76. Chichester, UK: John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781118720691.ch30.

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Buiatti, M., F. Gimelli, R. Venturo, P. Bogani, and T. Picconi. "Interclonal Variability Induced by in vitro and in vivo Propagation in a Vegetatively Propagated Plant, the Carnation." In Somaclonal Variations and Crop Improvement, 251–56. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-015-7733-5_27.

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Lee, Yung-I. "Vegetative Propagation of Orchids." In Springer Protocols Handbooks, 403–25. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7771-0_22.

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Conference papers on the topic "In vitro vegetative propagation"

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"Comparative transcriptome analysis of Syringa vulgaris vegetative apices in vivo and in vitro." In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-53.

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Batukaev, Abdulmalik. "IN VITRO MICROCLONAL PROPAGATION OF STRAWBERRIES AND EX VITRO ADAPTATION." In 19th SGEM International Multidisciplinary Scientific GeoConference EXPO Proceedings. STEF92 Technology, 2019. http://dx.doi.org/10.5593/sgem2019/6.1/s25.095.

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Kosolapova, Alena S., and Marina E. Lamberova. "Ultrasonic application during reception of biostimulators from vegetative raw material for cells and tissue culture in vitro." In 2009 International Conference and Seminar on Micro/Nanotechnologies and Electron Devices (EDM). IEEE, 2009. http://dx.doi.org/10.1109/edm.2009.5173975.

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Lu, Chengqun, and Baoling Huang. "Rapid propagation buds of Pinus massoniana in vitro." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE 2011). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965983.

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Shah, Ramisah Mohd, Nur Affida Abd Rahman, Iffah Hazirah Mohd Nawi, Nor Idzwana Mohd Idris, Rudiyanto, and Norhidayah Che Soh. "The Evaluation of Sexual and Vegetative Propagation of Medicinal Plant Christia vespertilionis (Butterfly Wing Plant)." In 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/aer.k.200325.040.

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"Induction of in vitro propagation in mature embrios of maize." In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-48.

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Abubakarov, H. G., E. A. Kalashnikova, R. N. Kirakosyan, and A. V. Shitikova. "In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.)." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-104.

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The results of optimizing the conditions for growing sweet potatoes in vitro are presented. It is shown that the mineral composition of the nutrient medium has a significant effect on the growth of side shoots. With the simultaneous formation of shoots, rooting of micro gears was observed.
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Zontikov, Dmitry. "IN VITRO PROPAGATION OF RUBUS CHAMAEMORUS L. AND RUBUS ARCTICUS L." In 14th SGEM GeoConference on NANO, BIO AND GREEN � TECHNOLOGIES FOR A SUSTAINABLE FUTURE. Stef92 Technology, 2014. http://dx.doi.org/10.5593/sgem2014/b61/s25.055.

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Leveque, Philippe, Alice Collin, Marylene Cueille, and Anne Perrin. "Electromagnetic, thermal and convection dosimetry for in vitro microwave exposure system." In 2006 1st European Conference on Antennas and Propagation (EuCAP). IEEE, 2006. http://dx.doi.org/10.1109/eucap.2006.4585016.

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Alatortseva, T. A. "Petchoa in vitro culture." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.015.

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Reports on the topic "In vitro vegetative propagation"

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Dick, Gary O., R. M. Smart, and Lynde L. Dodd. Propagation and Establishment of Native Plants for Vegetative Restoration of Aquatic Ecosystems. Fort Belvoir, VA: Defense Technical Information Center, June 2013. http://dx.doi.org/10.21236/ada582960.

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Zayova, Ely, Elisaveta Kitova, and Maria Geneva. Optimized Cultural Conditions for Rapid in Vitro Propagation and Conservation of Mentha piperita L. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, June 2021. http://dx.doi.org/10.7546/crabs.2021.06.18.

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Emek, Yelda, and Bengi Erdağ. In Vitro Propagation of Endemic and Endangered Rhaponticoides Mykalea Using Mature and Immature Zygotic Embryos. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, March 2020. http://dx.doi.org/10.7546/crabs.2020.03.08.

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Nasircilar, Ayse Gul. In vitro Clonal Propagation of Endemic Allium junceum Subs. tridentatum via Immature and Mature Embryo Culture Method. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, August 2021. http://dx.doi.org/10.7546/crabs.2021.08.18.

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The cassava seed system in Nigeria: Opportunities and challenges for policy and regulatory reform. International Potato Center, 2020. http://dx.doi.org/10.4160/23096586rtbwp20202.

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In many African countries south of the Sahara, farmers depend on the cultivation of vegetatively propagated crops (VPCs) for both consumption and commercial purposes. Yet yields for these crops remain at low levels due, in part, to the persistent use of low-quality planting material. Efforts to improve the quality of planting material exchanged in markets or through other channels are often hampered by the unique biological and economic characteristics of vegetative propagation—characteristics that distinguish VPCs from the major cereal crops that drive and shape the policy and investment choices made in many of these countries. This suggests that continued investment in new technologies and systems to produce, package, and distribute VPC planting materials will require customized policies and policy support if these systems are to supply farmers with quality planting material at any significant and sustained scale. This paper explores these issues in the context of the cassava seed system in Nigeria by drawing on (1) prior research, public policy documents, and government statistics; (2) key informant interviews and focus group discussions with seed system actors; and (3) a unique dataset from the 2015 Cassava Monitoring Survey of Nigeria (CMS). The paper examines the production and supply of cassava planting material, the influence of various quality assurance systems on production and supply, and the implications for smallholder farmers in Nigeria. We describe the market, non-market, and regulatory systems that shape the cassava seed market in Nigeria, focusing on effectiveness, influence, and reach. We then explore the ground realities—how farmers actually acquire and use cassava planting material—given the (weak) state of markets and regulation. This is followed by a discussion of alternative policy and regulatory approaches to managing and expanding the cassava seed system, emphasizing a more decentralized approach that prioritizes investment in innovative capacity at the community and enterprise levels.
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