Journal articles: 'In vitro vegetative propagation'

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Dubchak, Marcela, Olga Sultanova, and Viktor Bondarchuk. "Propagation of phytosanitary clones by in vitro culture." BIO Web of Conferences 34 (2021): 03003. http://dx.doi.org/10.1051/bioconf/20213403003.

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This article presents the process of accelerated reproduction of healthy clones of grapes, including the following stages: growing young shoots of the original plants of clones, introducing tops into in vitro culture, microclonal cuttings, adaptation of microplants to ex vitro culture, transplanting into cassettes with a soil substrate, transferring plants to a greenhouse for growing to the condition of vegetative seedlings and planting in a pre-propagation mother stock. For the successful implementation of each of the above operations in the SPIHVFT, a Cultural Complex has been equipped, consisting of a number of interconnected premises: a sterile box, a culture chamber and a vegetation chamber. The use of this Complex allows multiplying the required number of plants during the year, to grow vegetative seedlings by the spring of the next year and plant them in the pre-propagation “Pre-base” mother plant. After a year, grafted vegetative seedlings grown from the vines of the mother plant were used for laying the mother stock.

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Pierik, R. L. M., A. van Voorst, G. Booy, C. A. M. van Acker, C. L. C. Lelivelt, and J. C. de Wit. "* VEGETATIVE PROPAGATION OF ALSTROEMERIA HYBRIDS IN VITRO." Acta Horticulturae, no. 226 (June 1988): 81–90. http://dx.doi.org/10.17660/actahortic.1988.226.7.

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Deng, S. Y., I. M. Heap, and T. A. Klein. "In vitro vegetative propagation of Chinese cabbage." Plant Cell, Tissue and Organ Culture 26, no. 2 (August 1991): 135–39. http://dx.doi.org/10.1007/bf00036117.

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Pierik, R. L. M., H. H. M. Steegmans, A. A. Elias, O. T. J. Stiekema, and A. J. van der Velde. "* VEGETATIVE PROPAGATION OF SYRINGA VULGARIS L. IN VITRO." Acta Horticulturae, no. 226 (June 1988): 195–204. http://dx.doi.org/10.17660/actahortic.1988.226.22.

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Kanwar J, K., and S. Kumar. "In vitro propagation of Gerbera: A Review." Horticultural Science 35, No. 1 (February 12, 2008): 35–44. http://dx.doi.org/10.17221/651-hortsci.

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Gerbera has gained popularity in the past few years in many countries of the world and it is in great demand in the floral industry as cut flower as well as potted plant due to its beauty, colour, long vase life, and ability to rehydrate after long transportation. The most commercial cultivars are propagated through vegetative means by multiplication through divisions of clumps; however, the multiplication by this method is too slow to be commercially viable. To commercialize this crop and to meet the growing demand for planting material, tissue and organ culture techniques are being used as alternative methods for propagation in many countries. Most of the work has been carried on plant regeneration by adventitious organogenesis from capitulum, shoot tip, leaf, petiole and other parts of the plant. Attention should be paid to improve the technology to achieve 100% success in all species/cultivars to meet growing demands of the growers globally. From the literature, it is evident that gerberas are highly amenable to in vitro studies, as various explants were found to favourably respond to different culture media with different types and concentrations of growth regulators.

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Chalupa, V. "In vitro propagation of European larch (Larix decidua Mill.)." Journal of Forest Science 50, No. 12 (January 11, 2012): 553–58. http://dx.doi.org/10.17221/4656-jfs.

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Methods of in vitro propagation of Larix decidua are described in the paper. The influence of explant source, tree age, genotype, composition of nutrient media and phytohormones on in vitro propagation of Larix decidua has been investigated. Needles, isolated vegetative buds, shoot tips, zygotic embryos and cotyledons were used as initial explants. Axillary and adventitious bud cultures were used for fast in vitro shoot multiplication. Root induction was stimulated on low salt medium supplemented with auxin. After rooting and hardening off, micropropagated trees were planted in experimental plots. The growth of micropropagated trees was comparable with the height and diameter growth of trees originated from seeds.

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Dhawan, Vibha, and Sant S. Bhojwani. "In vitro vegetative propagation of Leucaena leucocephala (Lam.) de Wit." Plant Cell Reports 4, no. 6 (December 1985): 315–18. http://dx.doi.org/10.1007/bf00269887.

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Agbohessou, M., K. V. Salako, R. Idohou, R. C. Gbedomon, A. Hounkpèvi, F. J. Chadare, R. Glèlè Kakaï, and A. E. Assogbadjo. "Status of vegetative propagation of baobab: A review." African Crop Science Journal 28, s1 (October 2, 2020): 215–24. http://dx.doi.org/10.4314/acsj.v28i1.16s.

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The African baobab (Adansonia digitata L.) is a large tree of great socio-economic and cultural importance in Africa, with almost all the parts of the species used for various purposes. A major concern about baobab fruit pulp production is the long time it takes for first fruiting (about 15 years). Vegetative propagation offers several advantages with regard to consumers’ preferences and precociousness of fructification. The objective of this study was to synthesise existent knowledge related to vegetative propagation methods of baobab and examine future prospects for improving the species propagation. This will ultimately contribute to better integrate baobab-based agroforestry systems into the diversification and poverty alleviation programmes. It is clear that cutting, grafting and in vitro multiplication are the vegetative propagation methods already tested on baobab. The success of grafting methods ranges from 10 to 89%, depending on the technique used. The Murashige and Skoog environment, supplemented with or without growth regulator hormones is by far the best condition for the in vitro reactivity of baobab explants, regardless of their types. With regards to cuttings, the average success rates stand around 30% when Indole-3-butyric acid (IBA) hormone is used. Other approaches such as marcotting techniques are yet to be tested and data on fruit production using these techniques are still needed in order to determine the best promising method for rapid and efficient vegetative propagation of baobab.

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Pinto, José E. B. P., Clovis M. Souza, and W. R. Maluf. "In Vivo and in Vitro Propagation of Selected Cabbage (Brassica oleracea L. var. capitata) Clones." HortScience 31, no. 4 (August 1996): 628d—628. http://dx.doi.org/10.21273/hortsci.31.4.628d.

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Hybrid cabbage cultivars can be produced via seed-propagated self-incompatible (SI) inbred lines, or, alternatively, via vegetative propagation of SI clones. Cabbage clones differ in their ability to undergo vegetative propagation, a fact that appears to be related to the degree of differentiation of the axillary buds inside the head. A procedure for in vivo and in vitro propagation is described for cabbage clones known for difficulty in undergoing vegetative propagation. Cuttings from clonal families 800 (easy-to-propagate) and 007 (difficult to propagate) were immersed in indolebutyric acid (IBA—0, 5, 25, and 125 mg·L–1) + boric acid (100 mg·L–1) + sucrose (20 g·L–1) for 15 hours and maintained in glasshouses. Induction of roots was more effective with 125 mg·L–1 IBA supplemented with boric acid and sucrose. This treatment showed the highest frequency of rooting and the largest number of roots per cutting. The in vitro system of propagation was performed on the basal medium of Murashige and Skoog (MS), to which triadizuron (TDZ), benzyladeninepurine (BAP), and kinetin (Kin) were added in different combinations. TDZ was more effective than BAP or Kin in the promotion of shoot regeneration.

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Galopin, Gilles, François Beaujard, and Michel Gendraud. "Intensive production of juvenile cuttings by mother microplant culture in Hydrangea macrophylla "Leuchtfeuer"." Canadian Journal of Botany 74, no. 4 (April 1, 1996): 561–67. http://dx.doi.org/10.1139/b96-071.

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The vegetative propagation of hydrangea (Hydrangea macrophylla "Leuchtfeuer") should be explored through the architectural concepts of woody plants. The formation and continued culture of mother microplants in the greenhouse with high relative humidity and long days make up the new propagation method. Vegetative growth is characterized by the prolific production of homogenous cuttings as basal shoots in which the morphology of the axis is similar to that of plants produced by germination. There is considerable capacity for high levels of production, and it is a possible alternative to in vitro and traditional propagation. The morphogenetic basis of the functioning of these cultures is discussed in relation to juvenility and differentiation in woody plants. Keywords: morphogenesis, Hydrangea macrophylla, vegetative propagation, juvenility.

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Rousseau, M., S. Monfort, and M. Ferry. "IN VITRO VEGETATIVE PROPAGATION OF THE CANARY ISLANDS PALM (PHOENIX CANARIENSIS)." Acta Horticulturae, no. 486 (March 1999): 155–58. http://dx.doi.org/10.17660/actahortic.1999.486.20.

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Soressi, G. P., G. Cammareri, and M. E. Picarella. "IMPROVEMENT OF IN VITRO VEGETATIVE PROPAGATION TECHNIQUE IN TOMATO (SOLANUM LYCOPERSICUM)." Acta Horticulturae, no. 812 (February 2009): 283–88. http://dx.doi.org/10.17660/actahortic.2009.812.38.

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Reuveni, O., and S. Golubowicz. "TRIALS OF USING IN VITRO TECHNIQUES FOR VEGETATIVE PROPAGATION OF MANGOS." Acta Horticulturae, no. 455 (August 1997): 496–504. http://dx.doi.org/10.17660/actahortic.1997.455.64.

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Pierik, R. L. M., and F. A. A. Tetteroo. "Vegetative propagation of Begonia venosa Skan in vitro from inflorescence explants." Plant Cell, Tissue and Organ Culture 10, no. 2 (1987): 135–42. http://dx.doi.org/10.1007/bf00035911.

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Cañas, Luis A., Laura Carramolino, and Miguel Vicente. "Vegetative propagation of the olive tree from in vitro cultured embryos." Plant Science 50, no. 1 (January 1987): 85–90. http://dx.doi.org/10.1016/0168-9452(87)90034-3.

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Debnath, Samir C., and Usha Arigundam. "In Vitro Propagation Strategies of Medicinally Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.)." Agronomy 10, no. 5 (May 21, 2020): 744. http://dx.doi.org/10.3390/agronomy10050744.

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Lingonberry (Vaccinium vitis-idaea L.) is a health-promoting small fruit crop rich in antioxidant metabolites that helps to reduce the incidence of degenerative diseases. Being heterozygous, lingonberries cannot preserve genetic characteristics through seed propagation. Conventional vegetative propagation, although it produces true-to-type plants, is not economically viable. In vitro propagation can multiply plants much faster than conventional methods. A liquid cultures system under a bioreactor micropropagation system is of significant importance to increase the multiplication rates of in vitro-produced shoots. Enhanced vegetative growth and variation in biochemical constituents are observed in micropropagated plants. Clonal fidelity, although it may be a serious problem for commercial micropropagation, can be verified efficiently by molecular markers. The current review provides detailed and updated information on lingonberry micropropagation along with conventional methods and their effects on morphological, molecular and biochemical characteristics in micropropagated plants, filling the gap in literature.

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Ramirez, Marianela, Marek J. Krasowski, and Judy A. Loo. "Vegetative Propagation of American Beech Resistant to Beech Bark Disease." HortScience 42, no. 2 (April 2007): 320–24. http://dx.doi.org/10.21273/hortsci.42.2.320.

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The objective of this study was to develop vegetative propagation techniques—using tissue culture and grafting—for American beech (Fagus grandifolia) resistant to beech bark disease. Resterilizing the buds after excising bud scales reduced contamination of in vitro cultures derived from dormant buds. Application of a 7-day dark treatment before transferring shoots to the rooting medium improved rooting success. Plantlets gradually acclimatized to nonsterile growth conditions and set buds but failed to survive the dormant period. The application of 6-benzyladenine enhanced sprouting from roots collected from mature trees, but the excised shoots rooted poorly in vitro despite low contamination. Success of grafting scions from mature trees varied among genotypes and differed each year (30% in 2003, 12% in 2004, and 18% in 2005). Applying the growth hormone indole butyric acid to the scion before joining it to the rootstock did not increase grafting success. Survival of grafts was independent of rootstock age (1 or 2 years old). Grafting success increased when scion diameter was slightly larger than rootstock diameter. The rooting of sucker cuttings was successful for only one genotype. Critical steps, in which most failures in the propagation of American beech occur, are identified.

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Mofokeng, Motiki M., Hintsa T. Araya, S. O. Amoo, C. P. du Plooy, and P. W. Mashela. "Ex vitro vegetative propagation technique for sustainable utilization of Hypoxis hemerocallidea corms." South African Journal of Botany 139 (July 2021): 294–99. http://dx.doi.org/10.1016/j.sajb.2021.03.003.

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Wadl, Phillip A., Timothy A. Rinehart, Adam J. Dattilo, Mark Pistrang, Lisa M. Vito, Ryan Milstead, and Robert N. Trigiano. "Propagation for the Conservation of Pityopsis ruthii, an Endangered Species from the Southeastern United States." HortScience 49, no. 2 (February 2014): 194–200. http://dx.doi.org/10.21273/hortsci.49.2.194.

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Pityopsis ruthii is an endangered species endemic to the Hiwassee and Ocoee Rivers in Tennessee. As part of a recovery effort focused on P. ruthii, vegetative propagation and in vitro multiplication and seed germination techniques were developed. Plants were vegetatively propagated using greenhouse stock plants and wild-collected stems. Rooting occurred with and without auxin treatments but was greatest when 0.1% indole-3-butyric acid (IBA) talc was applied to the vegetative cuttings; rooting was lowest when flowering stems were used. Pro-Mix BX substrate provided the most consistent rooting. In vitro multiplication was accomplished by the removal of lateral shoots from in vitro-grown plants that were rooted on Murashige and Skoog (MS0) basal medium with 270 clones produced from a single individual after 4 months. Nineteen clones were transplanted and secured with bonded fiber matrix into their natural habitat and 14 survived for 1 year. To avoid genetic swamping of native populations with the introduction of large numbers of genetically identical individuals through clonal propagation, seed-based propagation efforts were explored. Open-pollinated seeds were collected, disinfested and germinated, and seedlings established on MS medium. Seeds were submersed in 70% ethanol for 1 minute and briefly flamed. Seeds were surface-sterilized in a range [10% to 50% (v/v)] Clorox® bleach solutions with vigorous shaking for 20 minutes, rinsed three times in sterile water, and germinated on MS0. Removal of pappus from seeds was required for successful disinfestations, but the bleach concentration was not critical. Successful propagation is a step toward the conservation and recovery of P. ruthii and should allow future reintroduction projects.

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Cardarelli, Mariateresa, Youssef Rouphael, Francesco Saccardo, and Giuseppe Colla. "An Innovative Vegetative Propagation System for Large-scale Production of Globe Artichoke Transplants. Part II. Propagation System Validation." HortTechnology 15, no. 4 (January 2005): 817–19. http://dx.doi.org/10.21273/horttech.15.4.0817.

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Research was conducted at the University of Tuscia (central Italy) to validate the propagation system for globe artichoke (Cynara cardunculus var. scolymus) described in a previous paper for a 1-year production cycle. The resulting globe artichoke plants were used in a 2-year field trial to investigate the field response of plantlets obtained with our propagation technique in comparison with plantlets produced by in vitro propagation and by offshoots harvested in commercial fields. The total number of artichoke plantlets obtained with our propagation system was 62.7 plantlets/m2 per year. In the first year, the globe artichoke production (bud number and fresh bud weight) was higher in plants obtained with our propagation system and by micropropagation than in those obtained from offshoots harvested in commercial fields. The production cost of plantlets obtained with our propagation technique was 52% lower than those of the micropropagated plantlets. This could lead to a significant reduction of production costs for artichoke growers, while preserving the advantages of in vitro propagation (disease-free plants and plant uniformity).

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Radojevic, L., P. Druart, and P. Boxus. "VEGETATIVE PROPAGATION OF ANDROGENOUS EMBRYOS OF HORSE CHESTNUT BY MERISTEM CULTURE IN VITRO." Acta Horticulturae, no. 212 (September 1987): 531–38. http://dx.doi.org/10.17660/actahortic.1987.212.81.

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Nahla, A., M. Eliraqy, A. El-Khasab, Roba Ismail, A. Esmail, S. Elsayh, Y. El-Aziz, E. El-Bassel, H. El-Ashry, and M. Gawish. "Comparative Studies on Vegetative and in vitro Propagation of Elite Selected Jojoba Strains." Asian Journal of Agricultural and Horticultural Research 2, no. 1 (August 13, 2018): 1–7. http://dx.doi.org/10.9734/ajahr/2018/42331.

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Gabryszewska, Eleonora. "Regeneration and growth of peony (Paeonia spp.) in vitro." Acta Agrobotanica 57, no. 1-2 (2013): 5–19. http://dx.doi.org/10.5586/aa.2004.001.

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Vegetative propagation of peony is usually carried out by grafting onto herbaceous peony roots (tree peony) or by dividing tuberous root clumps (herbaceous peony). However, conventional propagation is time-consuming and too slow for a large production and therefore in vitro methods have been developed. The regeneration ability of different organs of herbaceous and tree peony has been reported in this review. The role of exogenous and endogenous growth regulators in the differentiation and growth of shoots, roots and somatic embryos is presented. The problem of dormancy in the somatic embryos and in the shoots or plants propagated in vitro are also discussed.

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Hoesen, Djadja Siti Hazar. "PEMBENTUKAN TUNAS Lilium sp. SECARA EX VITRO DAN IN VITRO." Jurnal Teknologi Lingkungan 10, no. 2 (December 14, 2016): 183. http://dx.doi.org/10.29122/jtl.v10i2.1491.

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Buds, planlets and bulblets formation from excised bulbscales was the preferredmethod for vegetative propagation of Lilium sp (Liliaceae). The ex vitro techniqueswith Gibberellic acid (GA3) pretreatment was induced buds formation on scalescutting which planted on sterilized sand media. Buds rised from basal scales 7days after planted. However scales untreated GA3 obtained in 35-42 after planted.In vitro methods to promote buds initiated from bulbscales explants, was inducedon media MS (Murashige and Skoog) supplemented with GA3 1 mg/l. Media forinduced buds formation, MS contained Benzyl adenine (BA) 1 mg/l and 2 mg/lincreased multiple shoots formation significantly compared cultured on mediawithout BA. Roots growth improved on media contained NAA, but the highestplanlets achieved on cultured MS media without BA. Bulblets formation obtainedon media contained higher concentration of BA (5 mg/l).

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Debnath, Samir C. "Morphological Development of Lingonberry as Affected by In Vitro and Ex Vitro Propagation Methods and Source Propagule." HortScience 40, no. 3 (June 2005): 760–63. http://dx.doi.org/10.21273/hortsci.40.3.760.

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The growth and development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants and by shoot regeneration from excised leaves of micropropagated shoots, were studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. After 3 years of growth, the in vitro-derived plants produced more stems, leaves, and rhizomes than the conventional cuttings which rarely produced rhizomes. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favor rhizome production. This increase in vegetative growth and rhizome yield of in vitro-derived plants over stem cuttings varied among genotypes.

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Sharry, Sandra, Marina Adema, María A. Basiglio Cordal, Blanca Villarreal, Noelia Nikoloff, Valentina Briones, and Walter Abedini. "Propagation and Conservation of Native Forest Genetic Resources of Medicinal Use by Means of In Vitro and ex vitro Techniques." Natural Product Communications 6, no. 7 (July 2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600715.

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In Argentina, there are numerous native species which are an important source of natural products and which are traditionally used in medicinal applications. Some of these species are going through an intense extraction process in their natural habitat which may affect their genetic diversity. The aim of this study was to establish vegetative propagation systems for three native forestal species of medicinal interest. This will allow the rapid obtainment of plants to preserve the germplasm. This study included the following species which are widely used in folk medicine and its applications: Erythrina crista-galli or “seibo” (astringent, used for its cicatrizant properties and for bronchiolitic problems); Acacia caven or “espinillo” (antirheumatic, digestive, diuretic and with cicatrizant properties) and Salix humboldtiana or “sauce criollo” (antipyretic, sedative, antispasmodic, astringent). The methodology included the micropropagation of seibo, macro and micropropagation of Salix humboldtiana and the somatic embryogenesis of Acacia caven. The protocol for seibo regeneration was adjusted from nodal sections of seedlings which were obtained from seeds germinated in vitro. The macropropagation through rooted cuttings of “sauce criollo” was achieved and complete plants of this same species were obtained through both direct and indirect organogenesis using in vitro cultures. The somatic embryogenesis for Acacia caven was optimized and this led to obtain a high percentage of embryos in different stages of development. We are able to support the conservation of native forest resources of medicinal use by means of vegetative propagation techniques.

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Scaramuzzi, F., and A. D'Ambrosio. "ORGANOGENESIS AND PROPAGATION "IN VITRO" OF SIMMONDSIA CHINENSIS (LINK) SCHN. (JOJOBA) FROM VEGETATIVE FRAGMENTS." Acta Horticulturae, no. 227 (September 1988): 411–13. http://dx.doi.org/10.17660/actahortic.1988.227.77.

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Joseph, Nisha, and Vineeth V.K. "Conventional vegetative propagation and in vitro seed germination of endemic plant Colubrina travancorica Bedd." International Journal of Scientific Research in Biological Sciences 5, no. 4 (August 31, 2018): 134–39. http://dx.doi.org/10.26438/ijsrbs/v5i4.134139.

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SHUTO, Hirotoshi. "In vitro Vegetative Propagation of Gentian (Eustoma grandiflorum (GRISEB.) SCHINNER) by Single-node Culture." Plant tissue culture letters 11, no. 1 (1994): 76–77. http://dx.doi.org/10.5511/plantbiotechnology1984.11.76.

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Kretzschmar, Ulrich, and Dietrich Ewald. "Vegetative Propagation of 140-Year-Old Larix decidua Trees by Different in-vitro-Techniques." Journal of Plant Physiology 144, no. 4-5 (October 1994): 627–30. http://dx.doi.org/10.1016/s0176-1617(11)82149-8.

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Vidoy-Mercado, I., I. Imbroda-Solano, A. Barceló-Muñoz, M. A. Viruel, and F. Pliego-Alfaro. "THE INFLUENCE OF IN VITRO MICROGRAFTING ON VEGETATIVE PROPAGATION OF THE OLIVE CULTIVAR 'ARBEQUINA'." Acta Horticulturae, no. 949 (May 2012): 31–34. http://dx.doi.org/10.17660/actahortic.2012.949.2.

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Goller, Katarzyna, and Jan J. Rybczyński. "In vitro culture used for woody fern Cyathea australis (R. Br.) domin vegetative propagation." Acta Societatis Botanicorum Poloniae 64, no. 1 (2014): 13–17. http://dx.doi.org/10.5586/asbp.1995.002.

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Experiments have been carried out on vegetative multiplication of Cyathea australis. Cultures were initiated from spores collected from sporangium of mature fronds. Spores were sterilized in 3% chloramine with Tween and sown on Anderson (1984) medium supplemented with 80.0 mg/l of Ads and solidified by 8.0 g/l of agar. After three months of spore germination overgrowth of prothallia was observed. Multiplication of prothallia was stimulated by MS (1962) medium supplemented with 0.25 mg/l BAP, 0.50 mg/l IBA, 0.50 mg/l IAA, 1.00 mg/l GA3, 40.00 mg/l Ads and sucrose 30.0 g/l. Small drops of water were placed on the basal part of gametophyte in order to help ovary fertilization. After the next few weeks the first very fragile, small and green crosier emerged from the base of gametophyte. Perlite culture stimulated root formation and plant hardening to soil conditions.

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Krasnoperova, V. V., and I. L. Bucharina. "The study of the method of culture in vitro as a method of vegetative propagation of coniferous trees." Rossiiskaia selskokhoziaistvennaia nauka, no. 6 (December 15, 2019): 19–22. http://dx.doi.org/10.31857/s2500-26272019619-22.

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Research is aimed at improving the technology of vegetative propagation of coniferous trees in vitro by selecting optimal media for sterilizing the explants of vegetative parts of plants, and using growth regulators to obtain callus and stimulating the root formation of test-tube plants. The objects of study are the vegetative parts of conifers: the buds and parts of the annual shoots. Plant objects sterilized and planted on nutrient media of different composition according to the scheme of experiments. Laboratory studies were conducted, on the basis of which successful use of sodium hypochlorite was noted compared with other reagents for sterilization of explants. According to the results of the experiments, a Woody Plant Medium nutrient medium was identified which promotes the best survival of the explants of conifers. The effect on growth survival of explants and their viability in culture in vitro, growth regulators such as 2.4-dichlorophenoxyacetic acid and 2.4-dinitrophenol in nutrient media was also determined. With the further cultivation and reproduction of coniferous plants, this technique allows to obtain planting material that most economically preserved in the offspring the economically valuable traits and properties of the parent plant.

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Bulavin, Iliya, Valentina Brailko, and Irina Zhdanova. "In Vitro Rhizogenesis of The Lavandula angustifolia Cultivars." BIO Web of Conferences 24 (2020): 00017. http://dx.doi.org/10.1051/bioconf/20202400017.

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In production, shoot cuttings of lavender are most often used for vegetative propagation, however, this method does not promote high propagation rate. The most effective method is propagation by axillary or terminal buds that requires further plant rooting in vitro. Due to, objective of our work was analysis of lavender in vitro rhizogenesis. Investigation was performed on Lavandula angustifolia cultivars with different concentration of the growth regulators in medium. After18 Day, Only 1 mg/l NAA did not stimulate rhizogenesis for ‘Record’ and ‘Sineva’ cultivars. Root primordia formed from the cambium cells. Further root growth accompanied by cleavage of the core tissues and they appeared on the shoot surface. Morphologically, a root cap, meristem, elongation zone and zone with root hairs were identified in de novo formed root in vitro. Along with normally roots, the appearance of roots accreted along their periphery with free apexes and roots with the decreased meristem was also noted. For ‘Prima’ cultivar, highest values of the mitotic index were observed on the hormone-free half-strength MS medium and on ½MS medium with 1.0 mg/l NAA. Thus, our data showed that root morphogenesis for lavender cultivars depended on the plant material and culture medium.

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Liñán, Juana, Manuel Cantos, Javier Troncoso, José García, Antonio Fernández, and Antonio Troncoso. "Some propagation methods for cloning holm oak (Quercus ilex L.) plants." Open Life Sciences 6, no. 3 (June 1, 2011): 359–64. http://dx.doi.org/10.2478/s11535-011-0007-y.

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AbstractHolm oak (Quercus ilex L.), a typical evergreen tree of the Mediterranean area, is very important due to its ecological and economical values. Propagation of this species is extremely difficult and traditionally carried out only by seed germination. In this work, mature acorns were germinated in vitro and in peat substrate in aseptic and non-aseptic conditions. Explants from the seedlings obtained were propagated in vitro in WPM plus 4 µM BA. Plant regeneration was achieved from hypocotyls and root segments cultured in vitro on modified Gamborg medium plus 20 µM BA and 20 µM NAA. 13.8% of the hypocotyls and approximately 30% of the root segments developed both shoots and roots after 30 days of culture. Rooting of stem segments was obtained both in vitro and ex vitro by basal dipping in IBA solutions. Within ex vitro rooting, mother plant age had major influence on the percentage of rooting of the cuttings as the younger plants showed higher ability to root. In this way, Q. ilex plants could be propagated and cloned. The procedure described here would be a very useful tool for breeding programs since vegetative propagation of selected individuals can be achieved.

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Rogozińska, J. H., M. Gośka, and A. Kuźdowicz. "Induction of plants from anthers of Beta vulgaris cultured in vitro." Acta Societatis Botanicorum Poloniae 46, no. 3 (2015): 471–79. http://dx.doi.org/10.5586/asbp.1977.037.

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The influence of growth substances, saccharose and yeast extract on the differentiation of monogerm sugar beet and polygerm fodder beet anthers is studied. Callus and roots were found to form on the anthers. After subculture, callus derived from a well determined combination of growth substances differentiated into buds, from which plantlets were obtained in unlimited numbers. After rooting, they were transfermed to the soil where they continued to grow. This suggests the possibility of an adaptation of this method in vegetative propagation of beets.

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McCulloch, Steve. "1053 GENETIC STABILITY OF MICROPROPAGATED PLANTS." HortScience 29, no. 5 (May 1994): 579d—579. http://dx.doi.org/10.21273/hortsci.29.5.579d.

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Briggs Nurseries, Inc. has used micropropagation as method of vegetative propagation for over 20 years. Genetic stability and uniformity of plants that are produced and sold is of the utmost concern to the commercial plant propagator. Genetic stability may be accomplished by ensuring that all shoots formed in vitro are of axillary origin and by reducing shoot proliferation rates through the use of lower cytokinin concentrations in the culture medium. Excision and removal of callus during transfer is also necessary to ensure that shoots develop from axillary buds. Various factors that may influence genetic variability and its frequency of in vitro derived plants will be discussed with an emphasis on how to reduce them. Three sources of variation with tissue culture derived plants will also be reviewed (Swartz, 1991): a) source plant variability, b) genetic changes in vitro, and c) epigenetic or physiological adaptation.

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Gupta, Nikita, Vidhi Jain, Merin Rosy Joseph, and Siwani Devi. "A Review on Micropropagation Culture Method." Asian Journal of Pharmaceutical Research and Development 8, no. 1 (February 14, 2020): 86–93. http://dx.doi.org/10.22270/ajprd.v8i1.653.

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Micropropagation is a vegetative propagation conducted under controlled and aseptic conditions in the microenvironment of the culture vessel, which have the all growth requirements of a plant in the natural conditions. Recently different techniques of propagation have been developed which could facilitate large scale production of plants and for the improvement of the species. An overview on the in vitro propagation via meristem culture, callus culture and protoplast culture etc. are presented here. Today micropropagation techniques are applied in order to produce large numbers of new high-quality plants in a relatively short time and space, in low cost and can also be preserved.

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Li, Qiansheng, Mengmeng Gu, and Min Deng. "In Vitro Propagation of Oriental White Oak Quercus aliena Blume." Forests 10, no. 6 (May 28, 2019): 463. http://dx.doi.org/10.3390/f10060463.

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Quercus aliena Blume, also known as the oriental white oak, is a widespread species in temperate forests of East Asia with significant ecological and economical importance. Establishing an efficient vegetative propagation system is important for its germplasm conservation and breeding program. Protocols of micropropagation from shoot tips and nodal segments were investigated in order to produce uniform high-quality seedlings. Nodal segments from 18 month old seedlings were used as explants to initiate the aseptic culture. The highest bud proliferation was achieved by subculturing the explants on 1/2 strength woody plant medium (WPM) with 2.0 mg·L−1 BA. WPM with 0.5 mg·L−1 BA and 0.05 mg·L−1 IBA was the best medium for subculture to obtain the vigorous regenerated shoots in this experiment. Nodal segments without shoot tips had a higher adventitious bud proliferation rate than those with shoot tips. The highest rate (41.5%) of rooting in vitro was induced by using WPM with 1.0 mg·L−1 IBA and 5 g·L−1 activated charcoal. Ex vitro rooting by dipping the proliferated shoots with 500 mg·L−1 IBA solution, then transplanting directly to potting mix with 50% peat and 50% horticultural perlite fostered the highest rooting percentage and survival rate of the plantlets.

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Cho, Keun H., Veronica Y. Laux, Nathan Wallace-Springer, David G. Clark, Kevin M. Folta, and Thomas A. Colquhoun. "Effects of Light Quality on Vegetative Cutting and In Vitro Propagation of Coleus (Plectranthus scutellarioides)." HortScience 54, no. 5 (May 2019): 926–35. http://dx.doi.org/10.21273/hortsci13903-19.

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Coleus (Plectranthus scutellarioides) is an attractive and popular ornamental plant with propagation mainly achieved through vegetative cuttings. For commercial purposes, it is of interest to enhance the speed of establishment while maintaining high quality. Light quality has been shown to influence adventitious root development, so these experiments examined the effect of narrow-bandwidth light treatments on root growth and overall plant quality for seven coleus cultivars with vegetative cuttings in potting soil and one cultivar with shoot tip in vitro cultures onto Murashige and Skoog (MS) agar medium. During the 28 days of the propagation period, the cuttings grown under narrow-bandwidth red light (R; 663.4 nm at peak) more than doubled in the adventitious root number compared with those under blue light (B; 445.7 nm at peak) and green light (G; 530.0 nm at peak) in five cultivars. R light also increased fresh weight of the cuttings by 55.6% more than G light. In comparison, the cuttings grown under G light yielded significantly lower root and shoot dry mass than other light treatments. R light cuttings showed more dry mass content (9.63%) than those under white light (W; 437.4 nm and 559.5 nm at peak) and G light (7.85% and 5.86%, respectively). A positive correlation (R2 = 0.598, P < 0.001) was found between the formation of adventitious roots and gained fresh weight of cuttings. R light made the reddish color of leaves significantly stronger in most cultivars, whereas the cuttings exposed to G light became less vivid compared with other light conditions. When the shoot tips were propagated in vitro onto MS medium, R light treatment initiated the root development more rapidly than other lights, with significantly greater rooting rate (20.0% and 63.6%, respectively) at day 5 and 10. The shoot tips under R light also formed significantly more roots (12.3 per cutting) than those grown under narrow-bandwidth B light (5.8 per cutting). The shoot tips showed browning at an early stage and newly emerged leaves grew very compactly under B light. The combination of red and green light (R+G) increased more than twice as much roots and dry mass compared with W light. In addition, the R+G light led to morphological changes, including larger leaves and longer petioles and internodes than those in other light treatments. The exposure to R+G+B and B light made the shoots very compact for the 28 days of in vitro culture period and significantly increased the chlorophyll contents resulting in dark green leaves.

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Grigoriadou, Katerina, Virginia Sarropoulou, Nikos Krigas, Eleni Maloupa, and Georgios Tsoktouridis. "GIS-Facilitated Effective Propagation Protocols of the Endangered Local Endemic of Crete Carlina diae (Rech. f.) Meusel and A. Kástner (Asteraceae): Serving Ex Situ Conservation Needs and Its Future Sustainable Utilization as an Ornamental." Plants 9, no. 11 (October 29, 2020): 1465. http://dx.doi.org/10.3390/plants9111465.

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Conservation and sustainable exploitation of threatened endemic plants with medicinal and/or horticultural/ornamental value can be achieved through the development of effective propagation protocols. After unveiling the bioclimatic preferences of Carlina diae (Asteraceae) with geographic information systems (GIS), four propagation trials were conducted using seeds of this endangered local Cretan endemic for in vivo and in vitro germination, as well as seasonal vegetative propagation trials (softwood cuttings) and micropropagation (nodal explants). Seed germination was accomplished at a level of 77–90% in vivo (30 days) and 96% in vitro (10 days) using an MS medium with 2.9 μM gibberellic acid (GA3). The optimum treatments for cuttings’ rooting were 1000 and 2000 ppm indole-3-butyric acid (IBA) (11–16 roots, 2–3 cm long, 100% rooting) within 40 days in mist. In vitro shoot propagation exhibited a 2.8 proliferation rate after six successive subcultures on an MS medium with 2.9 μM GA3. Both ex vitro rooting and acclimatization were successful in 40 days, with 96% microshoot rooting and an equal survival rate. The GIS-facilitated effective species-specific propagation protocols developed in this study can consolidate the perspective of successful re-introduction of ex situ-raised material of C. diae into wild habitats and may serve its sustainable exploitation for high-added value ornamental products.

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Marsh, Thomas C., Eric S. Cole, Kathleen R. Stuart, Colin Campbell, and Daniel P. Romero. "RAD51 Is Required for Propagation of the Germinal Nucleus in Tetrahymena thermophila." Genetics 154, no. 4 (April 1, 2000): 1587–96. http://dx.doi.org/10.1093/genetics/154.4.1587.

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Abstract RAD51, the eukaryote homolog of the Escherichia coli recA recombinase, participates in homologous recombination during mitosis, meiosis, and in the repair of double-stranded DNA breaks. The Tetrahymena thermophila RAD51 gene was recently cloned, and the in vitro activities and induction of Rad51p following DNA damage were shown to be similar to that of RAD51 from other species. This study describes the pattern of Tetrahymena RAD51 expression during both the cell cycle and conjugation. Tetrahymena RAD51 mRNA abundance is elevated during macronuclear S phase during vegetative cell growth and with both meiotic prophase and new macronuclear development during conjugation. Gene disruption of the macronuclear RAD51 locus leads to severe abnormalities during both vegetative growth and conjugation. rad51 nulls divide slowly and incur rapid deterioration of their micronuclear chromosomes. Conjugation of two rad51 nulls leads to an arrest early during prezygotic development (meiosis I). We discuss the potential usefulness of the ciliates' characteristic nuclear duality for further analyses of the potentially unique roles of Tetrahymena RAD51.

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Bryan, Donita L., Michael A. Arnold, R. Daniel Lineberger, and W. Todd Watson. "Propagation Techniques for a Spineless Acacia wrightii." HortScience 40, no. 6 (October 2005): 1832–37. http://dx.doi.org/10.21273/hortsci.40.6.1832.

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Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

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Vidoy-Mercado, Isabel, Isabel Narváez, Elena Palomo-Ríos, Richard E. Litz, Araceli Barceló-Muñoz, and Fernando Pliego-Alfaro. "Reinvigoration/Rejuvenation Induced through Micrografting of Tree Species: Signaling through Graft Union." Plants 10, no. 6 (June 11, 2021): 1197. http://dx.doi.org/10.3390/plants10061197.

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Trees have a distinctive and generally long juvenile period during which vegetative growth rate is rapid and floral organs do not differentiate. Among trees, the juvenile period can range from 1 year to 15–20 years, although with some forest tree species, it can be longer. Vegetative propagation of trees is usually much easier during the juvenile phase than with mature phase materials. Therefore, reversal of maturity is often necessary in order to obtain materials in which rooting ability has been restored. Micrografting has been developed for trees to address reinvigoration/rejuvenation of elite selections to facilitate vegetative propagation. Generally, shoots obtained after serial grafting have increased rooting competence and develop juvenile traits; in some cases, graft-derived shoots show enhanced in vitro proliferation. Recent advances in graft signaling have shown that several factors, e.g., plant hormones, proteins, and different types of RNA, could be responsible for changes in the scion. The focus of this review includes (1) a discussion of the differences between the juvenile and mature growth phases in trees, (2) successful restoration of juvenile traits through micrografting, and (3) the nature of the different signals passing through the graft union.

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Thakur, Ram Bichari, and Joachim Schmerbeck. "Role of Tree Breeding in Timber and Wood Supply in World and India: Status and Outlook." Initiation 5 (April 19, 2014): 153–63. http://dx.doi.org/10.3126/init.v5i0.10266.

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Tree breeding is an important component of tree improvement which involves the application of genetic principles for the mass production of seedlings with desired traits in order to achieve higher productivity, better adaptability of the environment and vigorous growth rate. It helps in increasing yields and shortened rotations so it has a large potentiality to supply timber and wood demand of the world. Species choice, provenance selection and propagation method are the major aspects of tree breeding. Plus tree selection, progeny testing, provenance test and vegetative propagation have been used since early of civilization and often regarded as conventional tree breeding techniques while seed orchards, clonal propagation, somatic embryogenesis, micro-propagation or in-Vitro propagation, and biotechnology are modern tree breeding techniques. Different countries have been developing tree breeding techniques and achieving maximum benefits from it. Southeast Asia is using Acacia mangium, A. crassicarpa, Gmelina arborea, and Eucalyptus spp.; Populus deltoids, Casuarina equisetifolia, Eucalyptus spp. have been using by India; Teak has been vegetative propagated in Thailand; Salix babylonica has been growing in Greece for biomass production. Increasing yield and shortened rotation are the major prospects while loss of genetic diversity, higher production costs and requirement of constant upgrading are the major hindrances of tree breeding. DOI: http://dx.doi.org/10.3126/init.v5i0.10266 The Initiation 2013 Vol.5; 153-163

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Debnath, Samir C. "Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry." Canadian Journal of Plant Science 87, no. 4 (October 1, 2007): 911–22. http://dx.doi.org/10.4141/p06-131.

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Vacinium fruits are genetically heterozygous species characterized as “not coming true from seed”. Conventional methods for vegetative propagation of these species, although successful, are slow and labour-intensive, and few propagules can be produced from one plant of a selected clone or hybrid. Micropropagation techniques are important for clonal multiplication, germplasm im provement and gene conservation of Vaccinium fruits cultivated in Canada including blueberries, cranberries and lingonberries. In vitro propagation of these species using axillary bud proliferation and adventitious shoot regeneration has been investigated in a number of studies. Morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, and this dependence is genotype specific. The paper presents the progress in-depth of various aspects of the in vitro culture of Canadian Vaccinium species for their commercial production. Also discussed are techniques for clone rejuvenation and plant tissue culture for mass propagation of Canadian Vaccinium nuclear stocks. Key words: Blueberry, cranberry, lingonberry, micropropagation, regeneration, morphology

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Le, Diem Thi, and Mai Thi Bach Vo. "Influence of plant growth regulators on the rapid propagation buds of Dendrobium officinale Kimura et Migo." Science and Technology Development Journal - Natural Sciences 1, T2 (June 30, 2017): 29–38. http://dx.doi.org/10.32508/stdjns.v1it2.445.

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Dendrobium officinale Kimura et Migo has been known for a long time as a precious orchid, which is used for medicine and functional foods that are widely commercialized in the world. The nodal explants could be obtained in high efficiency, good quality and uniform seedlings on multiplication vegetative in vitro. They can be propagated in large quantities in a short time. The studied results showed that the nodal explants grew on MS medium + 10 % coconut milk + 25 g sucrose + 0.5 mg/L BA + 6 g agar/ liter create high buds. This buds were used to A B 1 cm 1,5 cm create bud clusters with the aim of improving in vitro vegetative. The best bud culture medium for the formation of clusters was MS + BA 2.0 mg/L + NAA 0.4 mg/L; MS + kinetin 1.5 mg/L + NAA 0.3 mg/L; MS + TDZ 2.0 mg/L + NAA 0.4 mg/L; MS + adenine 1.5 mg/L + NAA 0.3 mg/L. However, the addition of kinetin or TDZ to the culture medium needed a steps to extend the bud subculture after 45 days in culture. The best medium for the rooting is MS + NAA 0.5 mg/L.

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Bhadrawale, Deepti, Jay Prakash Mishra, and Yogeshwar Mishra. "An improvised in vitro vegetative propagation technique for Bambusa tulda: influence of season, sterilization and hormones." Journal of Forestry Research 29, no. 4 (December 22, 2017): 1069–74. http://dx.doi.org/10.1007/s11676-017-0569-2.

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Sottile, Francesco, Chiara Caltagirone, Cristiana Peano, Maria Beatrice Del Signore, and Ettore Barone. "Can the Caper (Capparis spinosa L.) Still Be Considered a Difficult-to-Propagate Crop?" Horticulturae 7, no. 9 (September 16, 2021): 316. http://dx.doi.org/10.3390/horticulturae7090316.

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As a perennial xerophytic shrub, characterized by plesiomorphic features, the caper (Capparis spinosa L.) is naturally spread throughout the Mediterranean basin and occupies an important ecological role, as well as an economic one, in traditional and specialized systems for commercial production. This species, in spite of its wide diffusion, is currently considered at risk of genetic erosion, mainly due to overgrazing and overharvesting for domestic uses and for trade. This situation is made more serious because of the lack of efficient propagation techniques, determining the caper as a “difficult-to-propagate species”. In this review, we report the main available sexual and vegetative propagation techniques with the aim of assessing whether, and to what extent, this criticality is still true for caper as a horticultural crop. In terms of seed propagation, germination rates have generally been considered quite low or unsatisfactory, and are also affected by hybridization phenomena that are likely to occur among both the wild and cultivated forms. The seeds show a physiological dormancy that can be lowered by adopting hormonal treatments, but in situ germination remains a critical phase. Vegetative propagation appears quite effective, mostly as related to in vitro techniques that allow caper cultivation that is no longer affected by propagation for an economic dissemination of the species in more intensive orchards. The research needs for Caper spinosa L. as a horticultural crop, especially in the field of genetic improvement and breeding, are also underlined.

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Zuzarte, M., A. M. Dinis, C. Cavaleiro, J. Canhoto, and L. Salgueiro. "Trichomes Morphology and Essential Oils Characterization of Field-Growing and In Vitro Propagated Plants of Lavandula pedunculata." Microscopy and Microanalysis 14, S3 (September 2008): 148–49. http://dx.doi.org/10.1017/s143192760808971x.

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The selection of native Lavandula species and their economic exploitation have increased in the last few years. Micropropagation techniques have been used as an alternative for vegetative propagation allowing the multiplication of selected genotypes and chemotypes. Our previous studies showed that the essential oils of Lavandula pedunculata have an important antifungal activity against dermatophyte strains. Therefore, a new line of investigation concerning the in vitro culture of this species is justified. In the present study we compare the morphology of the leaf trichomes and the chemical composition of their essential oils in both field-growing and in vitro propagated plants.

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Journal articles on the topic 'In vitro vegetative propagation'

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