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Journal articles on the topic 'In vivo bioluminescence imaging'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Liu, Tracy W., Seth T. Gammon, David Fuentes, and David Piwnica-Worms. "Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions." Cells 10, no. 3 (2021): 489. http://dx.doi.org/10.3390/cells10030489.

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A major obstacle in studying the interplay between cancer cells and the immune system has been the examination of proposed biological pathways and cell interactions in a dynamic, physiologically relevant system in vivo. Intravital imaging strategies are one of the few molecular imaging techniques that can follow biological processes at cellular resolution over long periods of time in the same individual. Bioluminescence imaging has become a standard preclinical in vivo optical imaging technique with ever-expanding versatility as a result of the development of new emission bioluminescent report

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Wiles, Siouxsie, Karen M. Pickard, Katian Peng, Thomas T. MacDonald, and Gad Frankel. "In Vivo Bioluminescence Imaging of the Murine Pathogen Citrobacter rodentium." Infection and Immunity 74, no. 9 (2006): 5391–96. http://dx.doi.org/10.1128/iai.00848-06.

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ABSTRACT Citrobacter rodentium is a natural mouse pathogen related to enteropathogenic and enterohemorrhagic Escherichia coli. We have previously utilized bioluminescence imaging (BLI) to determine the in vivo colonization dynamics of C. rodentium. However, due to the oxygen requirement of the bioluminescence system and the colonic localization of C. rodentium, in vivo localization studies were performed using harvested organs. Here, we report the detection of bioluminescent C. rodentium and commensal E. coli during colonization of the gastrointestinal tract in intact living animals. Biolumine

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Brovko, Lubov Y., and Mansel W. Griffiths. "Illuminating Cellular Physiology: Recent Developments." Science Progress 90, no. 2-3 (2007): 129–60. http://dx.doi.org/10.3184/003685007x215986.

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Bioluminescent methods are gaining more and more attention among scientists due to their sensitivity, selectivity and simplicity; coupled with the fact that the bioluminescence can be monitored both in vitro and in vivo. Since the discovery of bioluminescence in the 19th century, enzymes involved in the bioluminescent process have been isolated and cloned. The bioluminescent reactions in several different organisms have also been fully characterized and used as reporters in a wide variety of biochemical assays. From the 1990s it became clear that bioluminescence can be detected and quantified

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Jawhara, Samir, and Serge Mordon. "In Vivo Imaging of Bioluminescent Escherichia coli in a Cutaneous Wound Infection Model for Evaluation of an Antibiotic Therapy." Antimicrobial Agents and Chemotherapy 48, no. 9 (2004): 3436–41. http://dx.doi.org/10.1128/aac.48.9.3436-3441.2004.

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ABSTRACT A rapid, continuous method for noninvasively monitoring the effectiveness of several antibacterial agents in real time by using a model of wound infection was developed. This study was divided into three steps: (i) construction of a plasmid to transform Escherichia coli into a bioluminescent variant, (ii) study of the bioluminescent E. coli in vitro as a function of temperature and pH, and (iii) determination of the MIC and the minimal bactericidal concentration of sulfamethoxazole-trimethoprim (SMX-TMP). Finally, the efficacy of SMX-TMP was monitored in vivo in a cutaneous wound mode

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Pesnel, Sabrina, Smaïl Akkoul, Roger Ledée, et al. "Use of an Image Restoration Process to Improve Spatial Resolution in Bioluminescence Imaging." Molecular Imaging 10, no. 6 (2011): 7290.2011.00012. http://dx.doi.org/10.2310/7290.2011.00012.

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To improve spatial resolution in in vivo bioluminescence imaging, a photon scattering correction, image restoration method was tested. The chosen algorithm was tested on in vivo bioluminescent images acquired on three representative tumor models: subcutaneous, pulmonary, and disseminated peritoneal. Tumor size was chosen as a quantitative criterion, such that the tumor reference measurements (determined photographically or by computed tomography) were compared to those derived from bioluminescent images, before and after restoration. This technique allowed a significant reduction to be achieve

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Simonyan, Hayk, Chansol Hurr, and Colin N. Young. "A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions." Physiological Genomics 48, no. 10 (2016): 762–70. http://dx.doi.org/10.1152/physiolgenomics.00055.2016.

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Bioluminescence imaging is an effective tool for in vivo investigation of molecular processes. We have demonstrated the applicability of bioluminescence imaging to spatiotemporally monitor gene expression in cardioregulatory brain nuclei during the development of cardiovascular disease, via incorporation of firefly luciferase into living animals, combined with exogenous d-luciferin substrate administration. Nevertheless, d-luciferin uptake into the brain tissue is low, which decreases the sensitivity of bioluminescence detection, particularly when considering small changes in gene expression i

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Liu, Tracy W., Seth T. Gammon, and David Piwnica-Worms. "Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions." Cells 10, no. 3 (2021): 499. http://dx.doi.org/10.3390/cells10030499.

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Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well

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Kudo, Ayane, Haruka Osedo, Rahmawati Aisyah, et al. "Serum Amyloid A3 Promoter-Luciferase Reporter Mice Are Useful for Early Drug-Induced Nephrotoxicity Detection." International Journal of Molecular Sciences 25, no. 10 (2024): 5124. http://dx.doi.org/10.3390/ijms25105124.

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Early detection of drug-induced kidney injury is essential for drug development. In this study, multiple low-dose aristolochic acid (AA) and cisplatin (Cis) injections increased renal mRNA levels of inflammation, fibrosis, and renal tubule injury markers. We applied a serum amyloid A3 (Saa3) promoter-driven luciferase reporter (Saa3 promoter-luc mice) to these two tubulointerstitial nephritis models and performed in vivo bioluminescence imaging to monitor early renal pathologies. The bioluminescent signals from renal tissues with AA or CIS injections were stronger than those from normal kidney

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Rajashekara, Gireesh, David A. Glover, Menachem Banai, David O'Callaghan, and Gary A. Splitter. "Attenuated Bioluminescent Brucella melitensis Mutants GR019 (virB4), GR024 (galE), and GR026 (BMEI1090-BMEI1091) Confer Protection in Mice." Infection and Immunity 74, no. 5 (2006): 2925–36. http://dx.doi.org/10.1128/iai.74.5.2925-2936.2006.

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ABSTRACT In vivo bioluminescence imaging is a persuasive approach to investigate a number of issues in microbial pathogenesis. Previously, we have applied bioluminescence imaging to gain greater insight into Brucella melitensis pathogenesis. Endowing Brucella with bioluminescence allowed direct visualization of bacterial dissemination, pattern of tissue localization, and the contribution of Brucella genes to virulence. In this report, we describe the pathogenicity of three attenuated bioluminescent B. melitensis mutants, GR019 (virB4), GR024 (galE), and GR026 (BMEI1090-BMEI1091), and the dynam

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10

Zhang, Shuang, Chengcai Leng, Hongbo Liu, Kun Wang, and Jie Tian. "Fast in vivo bioluminescence tomography using a novel pure optical imaging technique." Journal of Innovative Optical Health Sciences 10, no. 03 (2017): 1750003. http://dx.doi.org/10.1142/s1793545817500031.

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Bioluminescence tomography (BLT) is a novel optical molecular imaging technique that advanced the conventional planar bioluminescence imaging (BLI) into a quantifiable three-dimensional (3D) approach in preclinical living animal studies in oncology. In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately, the prior structural information is commonly obtained from X-ray computed tomography (CT). This strategy requires a complicated hybrid imaging system, extensive post imaging analysis and involvement of ionizing radiation. Moreover, the overall robustn

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11

Saito-Moriya, Ryohei, Jun Nakayama, Genta Kamiya, et al. "How to Select Firefly Luciferin Analogues for In Vivo Imaging." International Journal of Molecular Sciences 22, no. 4 (2021): 1848. http://dx.doi.org/10.3390/ijms22041848.

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Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly B

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Endo, Mizuki, and Takeaki Ozawa. "Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission." International Journal of Molecular Sciences 21, no. 18 (2020): 6538. http://dx.doi.org/10.3390/ijms21186538.

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In vivo bioluminescence imaging (BLI), which is based on luminescence emitted by the luciferase–luciferin reaction, has enabled continuous monitoring of various biochemical processes in living animals. Bright luminescence with a high signal-to-background ratio, ideally red or near-infrared light as the emission maximum, is necessary for in vivo animal experiments. Various attempts have been undertaken to achieve this goal, including genetic engineering of luciferase, chemical modulation of luciferin, and utilization of bioluminescence resonance energy transfer (BRET). In this review, we overvi

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Hersh, Jessica, Yu-Ping Yang, Evan Roberts, et al. "Targeted Bioluminescent Imaging of Pancreatic Ductal Adenocarcinoma Using Nanocarrier-Complexed EGFR-Binding Affibody–Gaussia Luciferase Fusion Protein." Pharmaceutics 15, no. 7 (2023): 1976. http://dx.doi.org/10.3390/pharmaceutics15071976.

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In vivo imaging has enabled impressive advances in biological research, both preclinical and clinical, and researchers have an arsenal of imaging methods available. Bioluminescence imaging is an advantageous method for in vivo studies that allows for the simple acquisition of images with low background signals. Researchers have increasingly been looking for ways to improve bioluminescent imaging for in vivo applications, which we sought to achieve by developing a bioluminescent probe that could specifically target cells of interest. We chose pancreatic ductal adenocarcinoma (PDAC) as the disea

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Pajor, Katarzyna, Daniel Sypniewski, and Ilona Bednarek. "Bioluminescence as a tool in molecular biology." Postępy Higieny i Medycyny Doświadczalnej 71 (December 12, 2017): 0. http://dx.doi.org/10.5604/01.3001.0010.7011.

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Bioluminescence has been studied for many years by scientists. There are numerous mechanisms of that phenomenon; among them bacterial bioluminescence is the most frequently found in nature. This type of bioluminescence is determined by the appearance of lux operon, which encodes all elements necessary to produce light emission and it does not require any additional substrates supply. Another commonly found example of bioluminescence mechanism is performed by Photinus pyralis. Luciferase of P. pyralis named FLuc requires D-luciferin as a substrate. Bioluminescence is also characteristic for man

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Gross, Shimon, Seth T. Gammon, Britney L. Moss, et al. "Bioluminescence imaging of myeloperoxidase activity in vivo." Nature Medicine 15, no. 4 (2009): 455–61. http://dx.doi.org/10.1038/nm.1886.

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16

Jinchao Feng, Chenghu Qin, Kebin Jia, Shouping Zhu, Xin Yang, and Jie Tian. "Bioluminescence Tomography Imaging In Vivo: Recent Advances." IEEE Journal of Selected Topics in Quantum Electronics 18, no. 4 (2012): 1394–402. http://dx.doi.org/10.1109/jstqe.2011.2178234.

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17

Hardy, Jonathan, Matthias Edinger, Michael H. Bachmann, Robert S. Negrin, C. Garrison Fathman, and Christopher H. Contag. "Bioluminescence imaging of lymphocyte trafficking in vivo." Experimental Hematology 29, no. 12 (2001): 1353–60. http://dx.doi.org/10.1016/s0301-472x(01)00756-1.

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18

Kim, Jung Eun, Senthilkumar Kalimuthu, and Byeong-Cheol Ahn. "In Vivo Cell Tracking with Bioluminescence Imaging." Nuclear Medicine and Molecular Imaging 49, no. 1 (2014): 3–10. http://dx.doi.org/10.1007/s13139-014-0309-x.

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19

Chen, X., X. Zhang, and D. B. Kaufman. "BIOLUMINESCENCE IMAGING OF ISLET TRANSPLANTATION IN VIVO." Transplantation 78 (July 2004): 26. http://dx.doi.org/10.1097/00007890-200407271-00081.

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20

Juang, Jyuhn-Huarng, Chen-Ling Chen, Chen-Wei Kao, Shu-Ting Wu, and Chia-Rui Shen. "In Vivo Imaging of Immune Rejection of MIN6 Cells Transplanted in C3H Mice." Cells 13, no. 12 (2024): 1044. http://dx.doi.org/10.3390/cells13121044.

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Recently, we successfully utilized noninvasive magnetic resonance and bioluminescence imaging to track MIN6 cells subcutaneously transplanted in immunocompromised nude mice for up to 64 days. In this study, we further used bioluminescence imaging to investigate the immune rejection of MIN6 cells in immunocompetent C3H mice. A total of 5 × 106 luciferase-transfected MIN6 cells were implanted into the subcutaneous space of each nude or C3H mouse. After transplantation, hypoglycemia and persistent bioluminescence signals were observed in eight of eight (100%) nude mice and five of nine (56%) C3H

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Ferreira, Elisabeth, Landon B. Gatrell, Luke Childress, Hong Wu, and Ryan M. Porter. "A Transgenic Rat for Noninvasive Assessment of Chondrogenesis in Vivo." CARTILAGE 13, no. 2_suppl (2021): 1720S—1733S. http://dx.doi.org/10.1177/19476035211057243.

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Objective To support the preclinical evaluation of therapeutics that target chondrogenesis, our goal was to generate a rat strain that can noninvasively report endogenous chondrogenic activity. Design A transgene was constructed in which the dual expression of bioluminescent (firefly luciferase) and fluorescent (mCherry) reporters is controlled by regulatory sequences from rat Col2a1. Candidate lines were established on a Lewis background and characterized by serial bioluminescence imaging as well as ex vivo measurement of molecular reporter levels in several tissues. The sensitivity and speci

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Rocchetta, H. L., C. J. Boylan, J. W. Foley, et al. "Validation of a Noninvasive, Real-Time Imaging Technology Using Bioluminescent Escherichia coli in the Neutropenic Mouse Thigh Model of Infection." Antimicrobial Agents and Chemotherapy 45, no. 1 (2001): 129–37. http://dx.doi.org/10.1128/aac.45.1.129-137.2001.

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ABSTRACT A noninvasive, real-time detection technology was validated for qualitative and quantitative antimicrobial treatment applications. Thelux gene cluster of Photorhabdus luminescenswas introduced into an Escherichia coli clinical isolate, EC14, on a multicopy plasmid. This bioluminescent reporter bacterium was used to study antimicrobial effects in vitro and in vivo, using the neutropenic-mouse thigh model of infection. Bioluminescence was monitored and measured in vitro and in vivo with an intensified charge-coupled device (ICCD) camera system, and these results were compared to viable-

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Brock, Matthias, Grégory Jouvion, Sabrina Droin-Bergère, Olivier Dussurget, Marie-Anne Nicola, and Oumaïma Ibrahim-Granet. "Bioluminescent Aspergillus fumigatus, a New Tool for Drug Efficiency Testing and In Vivo Monitoring of Invasive Aspergillosis." Applied and Environmental Microbiology 74, no. 22 (2008): 7023–35. http://dx.doi.org/10.1128/aem.01288-08.

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ABSTRACT Aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number of drugs for treatment are available. A screening method for new antifungal compounds is urgently required, preferably an approach suitable for in vitro and in vivo studies. Bioluminescence imaging is a powerful tool to study the temporal and spatial resolutions of the infection and the effectiveness of antifungal drugs. Here, we describe the construction of a bioluminescent A. fumigatus strain by fusing the promoter of the glyceraldehyde-3-phosphate dehydrogenase

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Jeon, Yong Hyun, Yun Choi, Hyun Joo Kim, et al. "In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination." Molecular Imaging 6, no. 5 (2007): 7290.2007.00027. http://dx.doi.org/10.2310/7290.2007.00027.

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Recently, the use of a cancer deoxyribonucleic acid (DNA) vaccine encoding tumor-associated antigens has emerged as an immunotherapeutic strategy. In this study, we monitored tumor growth inhibition by pcDNA3-hMUC1 immunization in mice using optical imaging. To determine the anti-hMUC1-associated immune response generated by pcDNA3.1 or pcDNA3-hMUC1, we determined the concentration of interferon-γ (IFN-γ) protein and CD8+IFN-γ cell numbers among lymphocytes from the draining lymph nodes of mice immunized with pcDNA3.1 or pcDNA3-hMUC1. After subcutaneously injecting CT26/hMUC1-F luc into mice i

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Powers, Alvin, and E. Jansen. "In Vivo Bioluminescence Imaging to Assess Pancreatic Islets." Current Medicinal Chemistry-Immunology, Endocrine & Metabolic Agents 4, no. 4 (2004): 339–47. http://dx.doi.org/10.2174/1568013043357383.

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S�ling, Ariane, and Nikolai G. Rainov. "Bioluminescence imaging in vivo application to cancer research." Expert Opinion on Biological Therapy 3, no. 7 (2003): 1163–72. http://dx.doi.org/10.1517/eobt.3.7.1163.21271.

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Roda, Aldo, Massimo Guardigli, Elisa Michelini, and Mara Mirasoli. "Bioluminescence in analytical chemistry and in vivo imaging." TrAC Trends in Analytical Chemistry 28, no. 3 (2009): 307–22. http://dx.doi.org/10.1016/j.trac.2008.11.015.

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Söling, Ariane, and Nikolai G. Rainov. "Bioluminescence imaging in vivo – application to cancer research." Expert Opinion on Biological Therapy 3, no. 7 (2003): 1163–72. http://dx.doi.org/10.1517/14712598.3.7.1163.

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29

Rettig, Garrett R., Marie McAnuff, Dijie Liu, Ji-Seon Kim, and Kevin G. Rice. "Quantitative bioluminescence imaging of transgene expression in vivo." Analytical Biochemistry 355, no. 1 (2006): 90–94. http://dx.doi.org/10.1016/j.ab.2006.04.026.

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Alizai, Patrick Hamid, Lea Bertram, Daniela Kroy, et al. "Expression of VEGFR-2 during Liver Regeneration after Partial Hepatectomy in a Bioluminescence Mouse Model." European Surgical Research 58, no. 5-6 (2017): 330–40. http://dx.doi.org/10.1159/000479628.

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Background: Liver regeneration requires the formation of new blood vessels. Endothelial cell proliferation is stimulated by vascular endothelial growth factor (VEGF) and its receptor tyrosine kinase VEGFR-2. The aim of this study was to investigate VEGFR-2 expression in vivo during liver regeneration after partial hepatectomy (PHx). Methods: Transgenic VEGFR-2-luc mice were used in which the luciferase reporter gene was under control of the VEGFR-2 promoter. Following 2/3 PHx, the mice underwent in vivo bioluminescence imaging until the 14th postoperative day. Additionally, liver tissue was an

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Myers, Molly S., Elizabeth A. Kosmacek, Arpita Chatterjee, and Rebecca E. Oberley-Deegan. "CT vs. bioluminescence: A comparison of imaging techniques for orthotopic prostate tumors in mice." PLOS ONE 17, no. 11 (2022): e0277239. http://dx.doi.org/10.1371/journal.pone.0277239.

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Prostate cancer is one of the most diagnosed cancers in men in the United States. In mouse models, orthotopic tumors are favored for their biological relevance and simulation of growth in a microenvironment akin to that found in humans. However, to monitor the disease course, animal models require consistent and noninvasive surveillance. In vivo bioluminescent imaging has become a mainstay imaging modality due to its flexibility and ease of use. However, with some orthotopic prostate tumor models, bioluminescence fails to describe disease progression due to optical scattering and signal attenu

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Persyn, Aranka, Ona Rogiers, Matthias Brock, et al. "Monitoring of Fluconazole and Caspofungin Activity against In Vivo Candida glabrata Biofilms by Bioluminescence Imaging." Antimicrobial Agents and Chemotherapy 63, no. 2 (2018): e01555-18. http://dx.doi.org/10.1128/aac.01555-18.

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ABSTRACT Candida glabrata can attach to various medical implants and forms thick biofilms despite its inability to switch from yeast to hyphae. The current in vivo C. glabrata biofilm models only provide limited information about colonization and infection and usually require animal sacrifice. To gain real-time information from individual BALB/c mice, we developed a noninvasive imaging technique to visualize C. glabrata biofilms in catheter fragments that were subcutaneously implanted on the back of mice. Bioluminescent C. glabrata reporter strains (lucOPT 7/2/4 and lucOPT 8/1/4), free of auxo

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ZHENG, YING, QIAOYA LIN, HONGLIN JIN, JUAN CHEN, and ZHIHONG ZHANG. "VISUALIZATION OF HEAD AND NECK CANCER MODELS WITH A TRIPLE FUSION REPORTER GENE." Journal of Innovative Optical Health Sciences 05, no. 04 (2012): 1250028. http://dx.doi.org/10.1142/s1793545812500289.

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The development of experimental animal models for head and neck tumors generally rely on the bioluminescence imaging to achieve the dynamic monitoring of the tumor growth and metastasis due to the complicated anatomical structures. Since the bioluminescence imaging is largely affected by the intracellular luciferase expression level and external D-luciferin concentrations, its imaging accuracy requires further confirmation. Here, a new triple fusion reporter gene, which consists of a herpes simplex virus type 1 thymidine kinase (TK) gene for radioactive imaging, a far-red fluorescent protein (

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Matveeva, Evgenia G., Andrea K. Stoddard, Hui-Hui Zeng, et al. "Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response." International Journal of Molecular Sciences 23, no. 23 (2022): 14936. http://dx.doi.org/10.3390/ijms232314936.

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Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to transduce the level of free zinc as a ratio of emission intensities in the blue and orange portions of the spectrum. In addition to high sensitivity (below nanomolar levels) and selectivity, this approach allows both quantitative determination of “free” zinc ion (also termed “mobile” or “labile”) using

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Takakura, Hideo. "Molecular Design of d-Luciferin-Based Bioluminescence and 1,2-Dioxetane-Based Chemiluminescence Substrates for Altered Output Wavelength and Detecting Various Molecules." Molecules 26, no. 6 (2021): 1618. http://dx.doi.org/10.3390/molecules26061618.

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Optical imaging including fluorescence and luminescence is the most popular method for the in vivo imaging in mice. Luminescence imaging is considered to be superior to fluorescence imaging due to the lack of both autofluorescence and the scattering of excitation light. To date, various luciferin analogs and bioluminescence probes have been developed for deep tissue and molecular imaging. Recently, chemiluminescence probes have been developed based on a 1,2-dioxetane scaffold. In this review, the accumulated findings of numerous studies and the design strategies of bioluminescence and chemilum

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Saliu, Tolulope Peter, Nao Yazawa, Kotaro Hashimoto, et al. "Serum Amyloid A3 Promoter-Driven Luciferase Activity Enables Visualization of Diabetic Kidney Disease." International Journal of Molecular Sciences 23, no. 2 (2022): 899. http://dx.doi.org/10.3390/ijms23020899.

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The early detection of diabetic nephropathy (DN) in mice is necessary for the development of drugs and functional foods. The purpose of this study was to identify genes that are significantly upregulated in the early stage of DN progression and develop a novel model to non-invasively monitor disease progression within living animals using in vivo imaging technology. Streptozotocin (STZ) treatment has been widely used as a DN model; however, it also exhibits direct cytotoxicity to the kidneys. As it is important to distinguish between DN-related and STZ-induced nephropathy, in this study, we co

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Faley, Shannon L., Keiko Takahashi, Cornelia E. Crooke, et al. "Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis." Molecular Imaging 6, no. 5 (2007): 7290.2007.00029. http://dx.doi.org/10.2310/7290.2007.00029.

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Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis. We generated a transgenic reporter mouse, VEGF-GL, in which an enhanced green fluorescent protein-luciferase fusion protein is expressed under the control of a human VEGF-A promoter. The VEGF-GL mouse exhibited intense bioluminescence throughout the body at 1 week of age. The signals rapidly declined to a relatively low level as the mice grew. The adult VEGF-GL mouse showed restricted bioluminescence to the areas undergoing wound healing. In contrast, the VEGF-GL mice, which were crossed with mouse mammary tumor viru

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Tögel, Florian, Ying Yang, Ping Zhang, Zhuma Hu, and Christof Westenfelder. "Bioluminescence imaging to monitor the in vivo distribution of administered mesenchymal stem cells in acute kidney injury." American Journal of Physiology-Renal Physiology 295, no. 1 (2008): F315—F321. http://dx.doi.org/10.1152/ajprenal.00098.2008.

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Effective and targeted delivery of cells to injured organs is critical to the development of cell therapies. However, currently available in vivo cell tracking methods still lack sufficient sensitivity and specificity. We examined, therefore, whether a highly sensitive and specific bioluminescence method is suitable to noninvasively image the organ distribution of administered mesenchymal stem cells (MSCs) in vivo. MSCs were transfected with a luciferase/neomycin phosphotransferase construct (luc/neo-MSC). Bioluminescence of these cells was measured (charge-coupled device camera) after treatme

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Gaspar, Natasa, Giorgia Zambito, Iris J. C. Dautzenberg, et al. "NanoBiT System and Hydrofurimazine for Optimized Detection of Viral Infection in Mice—A Novel in Vivo Imaging Platform." International Journal of Molecular Sciences 21, no. 16 (2020): 5863. http://dx.doi.org/10.3390/ijms21165863.

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Reporter genes are used to visualize intracellular biological phenomena, including viral infection. Here we demonstrate bioluminescent imaging of viral infection using the NanoBiT system in combination with intraperitoneal injection of a furimazine analogue, hydrofurimazine. This recently developed substrate has enhanced aqueous solubility allowing delivery of higher doses for in vivo imaging. The small high-affinity peptide tag (HiBiT), which is only 11 amino-acids in length, was engineered into a clinically used oncolytic adenovirus, and the complementary large protein (LgBiT) was constituti

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Inoue, Yusuke, Shigeru Kiryu, Makoto Watanabe, Arinobu Tojo, and Kuni Ohtomo. "Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging." International Journal of Biomedical Imaging 2010 (2010): 1–6. http://dx.doi.org/10.1155/2010/471408.

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The peak signal or the signal at a predetermined, fixed time point afterD-luciferin injection may be used for the quantitative analysis of in vivo bioluminescence imaging. We repeatedly performed sequential bioluminescence imaging after subcutaneous injection ofD-luciferin in mice bearing subcutaneous tumors. The peak time in each measurement became shorter early after cell inoculation, presumably due to gradual establishment of intratumoral vasculature, and reached a plateau of about 10 min on day 10. Although the correlation between the signal at a fixed time point and the peak signal was hi

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Robiati, Louise, Paul Hindle, Sarah Stapley, and Hamish Simpson. "5 A new animal model for infected fracture non-union after external fixation of tibia with real-time in-vivo monitoring of infection." BMJ Military Health 169, no. 3 (2023): e3.5. http://dx.doi.org/10.1136/bmjmilitary-2023-rsmabstracts.5.

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BackgroundNon-union is a well-recognised complication after open fractures. Fifty percent of open extremity trauma returning from military operations developed non-union. Aetiology of non-union is multi- factorial, with infection reported as major contributory factor. The aim of this study was to develop an in-vivo model of infected fracture non-union managed with external fixation which allowed real time in-vivo monitoring of infection to evaluate potential therapeutic strategies.MethodTen male Wistar rats underwent application of external fixator and midshaft tibia osteotomy. Osteotomy sites

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Jiang, Tianyu, Jingwen Song, and Youming Zhang. "Coelenterazine-Type Bioluminescence-Induced Optical Probes for Sensing and Controlling Biological Processes." International Journal of Molecular Sciences 24, no. 6 (2023): 5074. http://dx.doi.org/10.3390/ijms24065074.

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Bioluminescence-based probes have long been used to quantify and visualize biological processes in vitro and in vivo. Over the past years, we have witnessed the trend of bioluminescence-driven optogenetic systems. Typically, bioluminescence emitted from coelenterazine-type luciferin–luciferase reactions activate light-sensitive proteins, which induce downstream events. The development of coelenterazine-type bioluminescence-induced photosensory domain-based probes has been applied in the imaging, sensing, and control of cellular activities, signaling pathways, and synthetic genetic circuits in

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Contag, Christopher H., and Michael H. Bachmann. "Advances in In Vivo Bioluminescence Imaging of Gene Expression." Annual Review of Biomedical Engineering 4, no. 1 (2002): 235–60. http://dx.doi.org/10.1146/annurev.bioeng.4.111901.093336.

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Rettig, Garrett R., Marie McAnuff, Dijie Liu, Ji-Seon Kim, and Kevin G. Rice. "401. Quantitative Bioluminescence Imaging of Transgene Expression In Vivo." Molecular Therapy 13 (2006): S154. http://dx.doi.org/10.1016/j.ymthe.2006.08.463.

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Mezzanotte, Laura, Moniek van ‘t Root, Hacer Karatas, Elena A. Goun, and Clemens W. G. M. Löwik. "In Vivo Molecular Bioluminescence Imaging: New Tools and Applications." Trends in Biotechnology 35, no. 7 (2017): 640–52. http://dx.doi.org/10.1016/j.tibtech.2017.03.012.

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Luo, Jian, Peggy Ho, Lawrence Steinman, and Tony Wyss-Coray. "Bioluminescence in vivo imaging of autoimmune encephalomyelitis predicts disease." Journal of Neuroinflammation 5, no. 1 (2008): 6. http://dx.doi.org/10.1186/1742-2094-5-6.

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Doyle, Timothy C., Stacy M. Burns, and Christopher H. Contag. "In vivo bioluminescence imaging for integrated studies of infection." Cellular Microbiology 6, no. 4 (2004): 303–17. http://dx.doi.org/10.1111/j.1462-5822.2004.00378.x.

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Maki, Shojiro. "Firefly Bioluminescence toward to In Vivo Imaging Technology." Nippon Laser Igakkaishi 37, no. 4 (2017): 448–53. http://dx.doi.org/10.2530/jslsm.jslsm-37_0035.

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Tiwari, Dhermendra K., Manisha Tiwari, and Takashi Jin. "Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging." Materials Advances 1, no. 5 (2020): 967–87. http://dx.doi.org/10.1039/d0ma00273a.

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Yull, Fiona E., Wei Han, E. Duco Jansen, et al. "Bioluminescent Detection of Endotoxin Effects on HIV-1 LTR-driven Transcription in Vivo." Journal of Histochemistry & Cytochemistry 51, no. 6 (2003): 741–49. http://dx.doi.org/10.1177/002215540305100605.

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We investigated the effects of Gram-negative bacterial lipopolysaccharide (LPS) on luciferase expression in transgenic reporter mice in which luciferase expression is driven by the nuclear factor κB (NF-κB)-dependent portion of the human immunodeficiency virus-1 (HIV-1) long terminal repeat (HIV-1 LTR). Using these mice, we dissected the sources of luciferase activity at the organ level by (a) assessing luciferase activity in organ homogenates, (b) bioluminescence imaging in vivo, and (c) bioluminescence imaging of individual organs ex vivo. Luciferin dosage was a critical determinant of the m

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