Relevant bibliographies by topics / In vivo Electrophysiology

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'In vivo Electrophysiology'

Author: Grafiati
Published: 4 June 2021
Last updated: 8 March 2025

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Journal articles on the topic "In vivo Electrophysiology"

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Adcock, J. "Airway nerves: in vivo electrophysiology." Current Opinion in Pharmacology 2, no. 3 (June 1, 2002): 280–82. http://dx.doi.org/10.1016/s1471-4892(02)00153-4.

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Volkov, Alexander G., Eunice K. Nyasani, Clayton Tuckett, Esther A. Greeman, and Vladislav S. Markin. "Electrophysiology of pumpkin seeds: Memristors in vivo." Plant Signaling & Behavior 11, no. 4 (February 29, 2016): e1151600. http://dx.doi.org/10.1080/15592324.2016.1151600.

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Erofeev, Alexander, Ivan Antifeev, Anastasia Bolshakova, Ilya Bezprozvanny, and Olga Vlasova. "In Vivo Penetrating Microelectrodes for Brain Electrophysiology." Sensors 22, no. 23 (November 23, 2022): 9085. http://dx.doi.org/10.3390/s22239085.

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In recent decades, microelectrodes have been widely used in neuroscience to understand the mechanisms behind brain functions, as well as the relationship between neural activity and behavior, perception and cognition. However, the recording of neuronal activity over a long period of time is limited for various reasons. In this review, we briefly consider the types of penetrating chronic microelectrodes, as well as the conductive and insulating materials for microelectrode manufacturing. Additionally, we consider the effects of penetrating microelectrode implantation on brain tissue. In conclusion, we review recent advances in the field of in vivo microelectrodes.
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Pavenstädt, Hermann, and Martin Bek. "Podocyte electrophysiology, in vivo and in vitro." Microscopy Research and Technique 57, no. 4 (May 7, 2002): 224–27. http://dx.doi.org/10.1002/jemt.10078.

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Ashbaugh, Ryan C., Lalita Udpa, Ron R. Israeli, Assaf A. Gilad, and Galit Pelled. "Bioelectromagnetic Platform for Cell, Tissue, and In Vivo Stimulation." Biosensors 11, no. 8 (July 25, 2021): 248. http://dx.doi.org/10.3390/bios11080248.

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Magnetogenetics is a new field that utilizes electromagnetic fields to remotely control cellular activity. In addition to the development of the biological genetic tools, this approach requires designing hardware with a specific set of demands for the electromagnets used to provide the desired stimulation for electrophysiology and imaging experiments. Here, we present a universal stimulus delivery system comprising four magnet designs compatible with electrophysiology, fluorescence and luminescence imaging, microscopy, and freely behaving animal experiments. The overall system includes a low-cost stimulation controller that enables rapid switching between active and sham stimulation trials as well as precise control of stimulation delivery thereby enabling repeatable and reproducible measurements.
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Berul, Charles I., Mark J. Aronovitz, Paul J. Wang, and Michael E. Mendelsohn. "In Vivo Cardiac Electrophysiology Studies in the Mouse." Circulation 94, no. 10 (November 15, 1996): 2641–48. http://dx.doi.org/10.1161/01.cir.94.10.2641.

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Liang, Yao-Wen, Ming-Liang Lai, Feng-Mao Chiu, Hsin-Yi Tseng, Yu-Chun Lo, Ssu-Ju Li, Ching-Wen Chang, Po-Chuan Chen, and You-Yin Chen. "Experimental Verification for Numerical Simulation of Thalamic Stimulation-Evoked Calcium-Sensitive Fluorescence and Electrophysiology with Self-Assembled Multifunctional Optrode." Biosensors 13, no. 2 (February 13, 2023): 265. http://dx.doi.org/10.3390/bios13020265.

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Owing to its capacity to eliminate a long-standing methodological limitation, fiber photometry can assist research gaining novel insight into neural systems. Fiber photometry can reveal artifact-free neural activity under deep brain stimulation (DBS). Although evoking neural potential with DBS is an effective method for mediating neural activity and neural function, the relationship between DBS-evoked neural Ca2+ change and DBS-evoked neural electrophysiology remains unknown. Therefore, in this study, a self-assembled optrode was demonstrated as a DBS stimulator and an optical biosensor capable of concurrently recording Ca2+ fluorescence and electrophysiological signals. Before the in vivo experiment, the volume of tissue activated (VTA) was estimated, and the simulated Ca2+ signals were presented using Monte Carlo (MC) simulation to approach the realistic in vivo environment. When VTA and the simulated Ca2+ signals were combined, the distribution of simulated Ca2+ fluorescence signals matched the VTA region. In addition, the in vivo experiment revealed a correlation between the local field potential (LFP) and the Ca2+ fluorescence signal in the evoked region, revealing the relationship between electrophysiology and the performance of neural Ca2+ concentration behavior. Concurrent with the VTA volume, simulated Ca2+ intensity, and the in vivo experiment, these data suggested that the behavior of neural electrophysiology was consistent with the phenomenon of Ca2+ influx to neurons.
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Annecchino, Luca A., and Simon R. Schultz. "Progress in automating patch clamp cellular physiology." Brain and Neuroscience Advances 2 (January 1, 2018): 239821281877656. http://dx.doi.org/10.1177/2398212818776561.

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Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand-gated channels, and showing the potential to play a pivotal role in drug discovery and biomedical research. Unfortunately, the cell suspension assays to which early systems were limited cannot recreate biologically relevant cellular environments, or capture higher order aspects of synaptic physiology and network dynamics. In vivo patch clamp electrophysiology has the potential to yield more biologically complex information and be especially useful in reverse engineering the molecular and cellular mechanisms of single-cell and network neuronal computation, while capturing important aspects of human disease mechanisms and possible therapeutic strategies. Unfortunately, it is a difficult procedure with a steep learning curve, which has restricted dissemination of the technique. Luckily, in vivo patch clamp electrophysiology seems particularly amenable to robotic automation. In this review, we document the development of automated patch clamp technology, from early systems based on multi-well plates through to automated planar-array platforms, and modern robotic platforms capable of performing two-photon targeted whole-cell electrophysiological recordings in vivo.
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TEPPER, JAMES M., and PHILIP M. GROVES. "In Vivo Electrophysiology of Central Nervous System Terminal Autoreceptors." Annals of the New York Academy of Sciences 604, no. 1 Presynaptic R (August 1990): 470–87. http://dx.doi.org/10.1111/j.1749-6632.1990.tb32013.x.

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Jayant, Krishna, Michael Wenzel, Yuki Bando, Jordan P. Hamm, Nicola Mandriota, Jake H. Rabinowitz, Ilan Jen-La Plante, et al. "Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology In Vivo." Cell Reports 26, no. 1 (January 2019): 266–78. http://dx.doi.org/10.1016/j.celrep.2018.12.019.

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Dissertations / Theses on the topic "In vivo Electrophysiology"

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Kodandaramaiah, Suhasa Bangalore. "Robotics for in vivo whole cell patch clamping." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/51932.

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Whole-cell patch clamp electrophysiology of neurons in vivo enables the recording of electrical events in cells with great precision, and supports a wide diversity of morphological and molecular analysis experiments important for the understanding of single-cell and network functions in the intact brain. However, high levels of skill are required in order to perform in vivo patching, and the process is time-consuming and painstaking. Robotic systems for in vivo patching would not only empower a great number of neuroscientists to perform such experiments, but would also open up fundamentally new kinds of experiment enabled by the resultant high throughput and scalability. We discovered that in vivo blind whole cell patch clamp electrophysiology could be implemented as a straightforward algorithm and developed an automated robotic system that was capable of performing this algorithm. We validated the performance of the robot in both the cortex and hippocampus of anesthetized mice. The robot achieves yields, cell recording qualities, and operational speeds that are comparable to, or exceed, those of experienced human investigators. Building upon this framework, we developed a multichannel version of “autopatcher” robot capable establishing whole cell patch clamp recordings from pairs and triplets of neurons in the cortex simultaneously. These algorithms can be generalized to control arbitrarily large number of electrodes and the high yield, throughput and automation of complex set of tasks results in a practical solution for conducting patch clamp recordings in potentially dozens of interconnected neurons in vivo.
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Suk, Ho-Jun. "Automated cell-targeted electrophysiology in vivo and non-invasive gamma frequency entrainment." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122429.

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Thesis: Ph. D., Harvard-MIT Program in Health Sciences and Technology, 2019
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 105-110).
Targeted patch clamp recording is a powerful method for characterizing visually identified cells in intact neural circuits, but it requires skill to perform. We found that a closed-loop real-time imaging strategy, which continuously compensates for cell movement while approaching the cell with a pipette tip, allows for the development of an algorithm amenable to automation. We built a robotic system that can implement this algorithm and validated that our system can automatically patch fluorophore-expressing neurons of multiple types in the living mouse cortex, with yields comparable to skilled human experimenters. By facilitating targeted patch clamp recordings in vivo, our robot may enable scalable characterization of identified cell types in intact neural circuits. Activities of individual neurons in neural circuits give rise to network oscillations, whose frequencies are closely related to specific brain states.
For example, network oscillations in the 30 - 90 Hz range, observed using electroencephalogram (EEG), are called gamma oscillations and increase during attention, memory formation, and recall. In Alzheimer's disease (AD), gamma oscillations are disrupted compared to healthy individuals. Recently, noninvasive visual and auditory stimulations at 40 Hz, called Gamma ENtrainment Using Sensory stimulus ("GENUS"), have been shown to positively impact pathology and improve memory in AD mouse models, with concurrent visual and auditory GENUS leading to a more widespread effect in the AD mouse brain compared to visual or auditory stimulation alone. However, it is unclear what effect such sensory stimulations would have on the human brain. To test for the safety and feasibility of GENUS in humans, we developed a device that can deliver 40 Hz light and sound stimulations at intensity levels tolerable to humans.
We found that our device can safely lead to steady 40 Hz entrainment in cognitively normal young (20 - 33 years old) and older (55 - 75 years old) subjects, with concurrent visual and auditory stimulation leading to stronger and more widespread entrainment than visual or auditory stimulation alone. These findings suggest that GENUS can be a safe and effective method for widespread 40 Hz entrainment, which may have therapeutic effects in people suffering from AD.
by Ho-Jun Suk.
Ph. D.
Ph.D. Harvard-MIT Program in Health Sciences and Technology
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Crnic, Agnes. "Effects of Acute and Sustained Administration of Vilazodone (EMD68843) on Monoaminergic Systems: An In Vivo Electrophysiological Study." Thesis, Université d'Ottawa / University of Ottawa, 2014. http://hdl.handle.net/10393/31498.

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Vilazodone is a partial 5-HT1A receptor agonist and a selective serotonin reuptake inhibitor (SSRI). Acute administration caused a dose-dependent decrease in dorsal raphe (DR) serotonin (5-HT) neuron firing rates. Vilazodone significantly decreased DR 5-HT neuronal firing following 2-day administration, which was shown to recover completely after 14-day administration. The 2-day administration of vilazodone significantly decreased firing in ventral tegmental area dopamine neurons; this effect persisted after 14-day treatment. The firing rate of norepinephrine neurons in the locus coeruleus was not significantly altered following 2-day treatment but did decrease following 14-day treatment. In the hippocampus, 14-day treatment with vilazodone significantly enhanced tonic activation, while having no effect on 5-HT reuptake. Vilazodone produced effects similar to conventional SSRIs while also inducing alterations in monoaminergic neurons that may be associated with its 5-HT1A properties and may have a role in the field of treatment resistant depression.
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Parent, Katherine L., and Katherine L. Parent. "Probing Neural Communication by Expanding In Vivo Electrochemical and Electrophysiological Measurements." Diss., The University of Arizona, 2017. http://hdl.handle.net/10150/626155.

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Neural communication is imperative for physical and mental health. Dysfunction in either ionic signaling or chemical neurotransmission can cause debilitating disorders. Thus, study of neurotransmission is critical not only to answer important fundamental questions regarding learning, decision making, and behavior but also to gain information that can provide insight into the neurochemistry of neurological disorders and lead to improved treatments. The work presented herein describes the development of techniques and instrumentation to enable advancements in neuroscientific inquiry. The effect of different temporal patterns and durations of simulation of the prefrontal cortex on dopamine release in the nucleus accumbens was examined and revealed a complex interaction that can help improve deep brain stimulation therapies. A measurement platform that combines electrophysiological and electrochemical techniques is described. The instrumentation is capable of concurrent monitoring of neural activity and dopamine release in vivo and in freely moving rodents. Analysis techniques to allow absolute quantification of tonic dopamine concentrations in vivo are detailed and the temporal resolution of the technique was vastly improved from ten minutes to forty seconds. An instrument that can simultaneously probe both dopamine and serotonin dynamics in either of their two temporal modes of signaling (tonic and phasic) using either fast-scan cyclic voltammetry or fast-scan controlled-adsorption voltammetry at two individually addressable microelectrodes is described. Together these new tools represent a significant step forward in the field of neuroanalytical chemistry by enable multiple brain regions, signaling modes (ionic flux in addition to both tonic and phasic neurotransmission), neurochemicals, and to be measured together.
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Pitcher, Toni Leigh, and n/a. "In vivo electrophysiology of striatal spiny projection neurons in the spontaneously hypertensive rat (SHR)." University of Otago. Department of Anatomy & Structural Biology, 2007. http://adt.otago.ac.nz./public/adt-NZDU20070321.114819.

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The aim of this thesis was to investigate neuronal cellular mechanisms that may underlie the behavioural characteristics of the spontaneously hypertensive rat strain (SHR). The SHR was developed by selective breeding for elevated blood pressure and is also described as having increased levels of locomotor behaviour compared to its normotensive control strain, the Wistar-Kyoto. This hyperactivity and other behaviours, including altered sensitivity to reinforcement, have been used to model aspects of behaviour displayed in attention deficit hyperactivity disorder. In vivo intracellular recording of striatal spiny projection neuron activity in urethaneanaesthetised animals from three genetically related strains: the SHR, Wistar-Kyoto and standard Wistar, was employed to measure basic cellular properties and cellular mechanisms of reward-related learning. This population of neurons was chosen because alterations in their activity can influence behaviour and they are known to show cellular changes (synaptic plasticity) that are associated with learning. Cellular properties were measured in 71 neurons. Comparison between strains revealed a significant difference in action potential amplitude and duration between the SHR and Wistar-Kyoto strains. Interestingly, when measured at a later time, in a different sample of rats, the SUR action potential amplitude and duration were significantly different from the earlier sample. A change in the membrane potential repolarisation rate following action potential firing also occurred over this time. Twenty-nine of these neurons were also used in a study investigating the neuronal responses to a low dose of amphetamine (0.5 mg/kg). Changes were observed in some cellular properties following intraperitoneal administration of amphetamine. Synaptic plasticity at the corticostriatal synapses is sensitive to the timing of dopamine release in relation to cortical input. In anaesthetised preparations the spiny projection neuron membrane potential fluctuates between hyperpolarised (DOWN) and depolarised (UP) states, which reflect the level of cortical input. During the present study the responses of nine neurons to the induction of cortical spreading depression were observed to investigate the suitability of this method for use during synaptic plasticity experiments. Spiny projection neurons showed unpredictable responses to cortical spreading depression, therefore this method was not used further. Corticostriatal synaptic plasticity was induced in sixteen spiny projection neurons from two strains: SHR and Wistar. High frequency stimulation of the dopamine neurons in the substantia nigra, during the DOWN-state, did not induce any significant changes in corticostriatal synaptic efficacy. This was also true when high frequency stimulation of dopamine neurons was applied during the UP-state in neurons from the SHR strain. This thesis represents the first in vivo intracellular study of neuronal physiology in the SHR and Wistar-Kyoto rat strains. Results revealed action potential differences between these two behaviourally distinct rat strains. Synaptic mechanisms thought to underlie reward-related learning were not different between the SHR and Wistar strains, although the observed levels of plasticity were inconsistent with previous literature.
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Malezieux, Meryl. "Dynamique intracellulaire des cellules pyramidales de CA3 dans l'hippocampe pendant les états de veille." Thesis, Bordeaux, 2018. http://www.theses.fr/2018BORD0317/document.

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Les états de veille sont composés d’états cérébraux distincts, corrélés avec différents comportements et caractérisés par des oscillations spécifiques observables dans le potentiel de champ local (Local Field Potential, LFP). Bien que les différents états cérébraux et leur signature dans le LFP aient été caractérisés, les mécanismes cellulaires sous-jacents restent à ce jour peu connus. Des changements des propriétés de neurones uniques seraient corrélés avec, et pourraient participer à la génération de ces changements d’états cérébraux. L’activité coordonnée et synchronisée de neurones facilite certains processus cognitifs tels que la mémoire. L’hippocampe joue un rôle essentiel dans les mémoires spatiale et épisodique, et dans l’hippocampe, CA3 est important pour la formation d’associations facilitant l’encodage rapide de la mémoire. De plus, les informations provenant du cortex entorhinal, du gyrus denté, et de CA3 même sont comparées et intégrées dans CA3 avant d’être transmises à CA1. Lors de périodes de repos, le LFP hippocampique présente une activité large et irrégulière (Large Irregular Activity, LIA), ponctuée par des oscillations plus rapides, les sharp-wave ripples, jouant un rôle dans la consolidation de la mémoire. Lors de périodes exploratoires, le LFP hippocampique oscille aux fréquences theta (6-12 Hz) et gamma (30-100 Hz). Les cellules pyramidales (CP) de CA3 jouent un rôle important dans chacun de ces états ; elles sont nécessaires pour les sharp wave lors de périodes de repos, et les oscillations gamma lors de comportements exploratoires. Dans le but d’étudier les modulations intracellulaires des CP de CA3, nous avons réalisé des enregistrements de patch-clamp en configuration cellule entière chez l’animal éveillé. Nous avons associé ces enregistrements avec des mesures du diamètre pupillaire et de la vitesse de locomotion de l’animal, ainsi qu’avec l’enregistrement de l’activité oscillatoire du LFP dans l’hippocampe. Nos résultats montrent que certaines CP de CA3 sont sensibles à la modulation intracellulaire lors de différents rythmes hippocampiques, et ont tendance à diminuer leur potentiel de membrane moyen, leur excitabilité, leur variance et leur décharge de potentiel d’action lors des oscillations theta par rapport aux périodes de LIA. De futures études permettront de déterminer si ces changements sont dus à des changements d’entrées synaptiques et/ou de neuromodulateurs. Ces modulations pourraient jouer un rôle dans l’émergence des rythmes oscillatoires du LFP, et permettre à CA3 de réaliser différentes fonctions mnésiques à différents moments
Wakefulness is comprised of distinct brain states, correlated with different behaviors and characterized by specific oscillatory patterns in the local field potential (LFP). While much work has characterized different brain states and their LFP signatures, the underlying cellular mechanisms are less known. Changes in single cell properties are thought to correlate with and possibly result in these changes in brain state. Synchronized and coordinated activity among distributed neurons supports cognitive processes such as memory. The hippocampus is essential for spatial and episodic memory, and within the hippocampus, area CA3 is important for rapid encoding of one-trial memory. Additionally, CA3 is the site where information from the entorhinal cortex, dentate gyrus, and CA3 itself is compared and integrated before output to CA1. During quiet wakefulness, the hippocampal LFP displays large irregular activity (LIA) punctuated by sharp-wave ripples, which play a role in memory consolidation. During exploratory behaviors, hippocampal LFP oscillates at both theta and gamma frequencies. CA3 pyramidal cells (PCs) play an important role in each of these brain states; they are necessary for both sharp waves during quiet wakefulness and for gamma oscillations during exploratory behavior. We explored the changes that occur in the intracellular dynamics of CA3 PCs during changes in brain state, by using whole-cell patch-clamp recordings from CA3 PCs in awake head-fixed mice. We combined those recordings with measurements of pupil diameter, treadmill running speed and LFP recordings of oscillatory activity. Our findings show that some CA3 PCs are prone to intracellular modulation during brain rhythms, and tend to decrease their average membrane potential, excitability, variance and output firing during theta as compared to LIA. Future studies will demonstrate whether these effects are due to changes in synaptic and/or neuromodulatory inputs. This modulation at the single-cell level in CA3 could play a role in the emergence of oscillations, and underlie the ability of CA3 to perform different memory functions during different brain states
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Pollnow, Stefan [Verfasser], and Olaf [Akademischer Betreuer] Dössel. "Characterizing Cardiac Electrophysiology during Radiofrequency Ablation : An Integrative Ex vivo, In silico, and In vivo Approach / Stefan Pollnow ; Betreuer: Olaf Dössel." Karlsruhe : KIT Scientific Publishing, 2019. http://d-nb.info/1186145404/34.

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Shim, Stacey. "Alterations of the Monoaminergic Systems in the Rat Brain by Sustained Administration of Carisbamate and Lamotrigine." Thèse, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/23478.

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Carisbamate (CRS) and lamotrigine (LTG) are anticonvulsants which act mainly on neuronal voltage-gated sodium channels, that have been shown to have antidepressant-like effects in animal models of depression. In vivo electrophysiological recordings were carried out following 2 and 14 days of CRS or LTG administration. Overall firing activity in the dorsal raphe, locus coeruleus and ventral tegmental area were decreased with CRS. Similarly, a decrease in the dorsal raphe was also observed with LTG. Despite these presynaptic decreases in firing activity, both anticonvulsants exhibited significant enhancement of serotonergic transmission in the hippocampus as demonstrated by increased tonic activation of postsynaptic 5-HT1A receptors. This may be attributed to the observed desensitization of the terminal 5-HT1B autoreceptors. This study suggests that the enhanced serotonergic effect may be associated with an antiglutamatergic effect, and may contribute to the antidepressant-like effect of CRS in the forced swim test and the antidepressant properties of LTG.
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Klimas, Aleksandra. "High-Throughput All-Optical Cardiac Electrophysiology| Design, Validation, and Applications in vitro and in vivo." Thesis, The George Washington University, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10621781.

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Biological systems are inherently dynamic, requiring active interrogation and recording to provide a full understanding of their underlying mechanics. In order to fully characterize such a system, both readily quantifiable signals as well as a means of dynamic control are necessary. In the heart, the propagation of electrical waves driving contraction are mediated by the flow of ions through various ion channels working in concert to drive de- and re-polarization of the cell membrane. Typically, the culprit of electrical dysfunction in the heart is due to some disruption of normal function of one or more of these ion channels. In order to study these complex electrical disturbances, known as arrhythmias, high spatiotemporal resolution imaging and interrogation are necessary.

Traditional methods of interrogation have relied on the use of electrodes and patch clamp methods, which are inherently low throughput and have limited spatial resolution. Additionally, these approaches do not lend well for in vivo use. While studies of cardiac tissue have also employed optical mapping techniques where voltage- or calcium-sensitive fluorescent reporters provide detailed information about cell activation, repolarization, and wave propagation maps, stimulation has remained primarily limited to electrical means. However, recently developed optogenetic tools provide a means of high-spatiotemporal resolution (and potentially tissue-type specific) means of interrogation. By combining both of these methods, high-spatiotemporal dynamic characterization of cardiac electrophysiology can be achieved.

Here we present how all-optical approaches can be achieved via employing optogenetics in order to explore cardiac electrophysiology at the in vitro as well as in vivo scale. The main optical design is first implemented for in vitro use, where we demonstrate how OptoDyCE, our all-optical dynamic cardiac electrophysiology platform, can be used to screen drug effects in both isolated primary myocytes and human induced pluripotent stem-cell derived cardiomyocytes (hiPSC-CMs) grown in monolayers and 3D tissue constructs. We then characterize an upgraded version of OptoDyCE, capable of simultaneous imaging of membrane voltage and intracellular calcium signals. The system is used for screening of 12 blinded compounds to demonstrate how the platform can used for pro-arrhythmia prediction at the high-throughput (HT) scale. All compounds were properly identified as ‘safe’ or ‘unsafe’ using the multi-parameter endpoints, made possible with high-spatiotemporal resolution recordings under spontaneous and paced conditions. To further demonstrate how all-optical approaches improve proarrhythmia prediction, we tested vanoxerine, a compound that failed Phase III clinical trials, and demonstrate OptoDyCE’s ability to easily identify the compound as pro-arrhythmic, unlike techniques employing patch clamp and in silico modeling that deemed the compound safe for use in humans. As hiPSC-CMs provide a novel testbed for drug testing and disease modeling, we then use OptoDyCE to characterize these cells, both in terms of their potential immaturity (a common criticism) and their ability to recapitulate genetic diseases for use in disease modeling. Finally, the requirements for translating OptoDyCE for in vivo use are considered, and successful demonstration in vivo expression of ChR2 in the rat heart by employing systemic viral delivery, providing a model for development and testing of an optical system in intact tissue and for long-term use in behaving animals. Ultimately, we demonstrate the OptoDyCE platform has capacity to revolutionize pre-clinical drug testing, reduce cost, reduce animal use, and make clinically implemented personalized medicine an obtainable goal.

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Squirrell, Daniel. "An in vivo electrophysiological and computational analysis of hippocampal synaptic changes in the Alzheimer's disease mouse." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/an-in-vivo-electrophysiological-and-computational-analysis-of-hippocampal-synaptic-changes-in-the-alzheimers-disease-mouse(de740023-7d91-418a-8c88-1141b3cd81f3).html.

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Alzheimer’s disease (AD) is a neurodegenerative disorder resulting in the decline of cognitive function, memory formation and retrieval, and abrupt changes in personality. Damage to brain networks occur during prodromal stages of AD, prior to the development of clinical symptoms of dementia. Further characterising this state and identifying reliable biomarkers for early detection are priorities in AD research. I characterised neuronal changes within the dorsal CA1 and subiculum regions of the hippocampal formation (HF) in the well-characterised 3xTgAD mouse model of AD. These regions are well-established sites for early neurodegeneration in both AD patients and AD animal models. We inserted multi-electrode recording arrays into CA1 and subiculum of urethane anaesthetised 3xTgAD mice and recorded spontaneous local field potential activity. Using traditional and novel information theoretic approaches, I determined the information carrying capacity of the CA1- subiculum network during different network rhythms, and how this altered with age and AD-like pathology. A bipolar stimulating electrode was inserted into CA1, allowing the assessment of synaptic integrity between CA1 and subiculum. Results showed that synaptic and network changes occur in CA1 and subiculum during the early stages of AD-like pathology and correlates with the development of intracellular beta-amyloid. There is a progressive breakdown in synaptic facilitation as early as 3 months in the 3xTgAD mouse. These data support an advanced ageing-like phenotype in AD model mice, with an enhanced age/pathology-dependent breakdown in neuronal communication compared to age-matched controls. In agreement with other studies, 3xTgAD mice demonstrate evidence of pathology-related changes in the network rhythms of the HF. 3xTgAD mice show an increase in the power of alpha and beta rhythms, and a concurrent reduction in the power of delta oscillations. Application of novel information theoretic techniques results in a breakdown in the information carrying capacity of the hippocampal system. This deficit manifests as a reduction in information flow during delta-dominant periods of EEG rhythms, with a specific reduction during slow-wave ripple activity. This change in neuronal communication correlates with the onset of memory-retention/consolidation deficits. These network changes are complex, with alterations in the information carrying capacity of the system during theta rhythms at 6 months, and during slow-wave components by 9 months in the 3xTgAD mouse. This study provides the first evidence of an early and progressive decline in neuronal connectivity and communication that correlates with changes in cognition in the 3xTgAD mouse. Application of novel analytical techniques to multi-site EEG recording revealed early and measureable changes in information processing during the onset of AD-like pathology. These are important new biomarkers for early AD characterisation.
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Books on the topic "In vivo Electrophysiology"

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Qasim, Salman Ehtesham. In vivo electrophysiology in humans reveals neural codes for space and memory. [New York, N.Y.?]: [publisher not identified], 2021.

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Tseng, Hua-an, Richie E. Kohman, and Xue Han. Optogenetics and Electrophysiology. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0009.

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Throughout the history of neuroscience, electrophysiological and imaging techniques have been utilized to observe neural signals at various spatial and temporal scales. However, it has been difficult to manipulate the activity of specific cells or neural circuits with the spatial and temporal resolutions relevant to neural coding. A novel technique called optogenetics, has recently been developed to control the activity of specific cells. This technique allows rapid and reversible optical activation or silencing of specific cells, which have been genetically transduced with light-sensitive molecules. The development of microbial opsin-based optogenetic molecular sensors has made optogenetics easily adaptable in various in vivo and in vitro preparations, and the technique has already been applied to understand neural circuit mechanisms of many behaviors and diseases. Here, we provide an introduction to optogenetics, the practical concerns in using the technique in vivo, and examples of applications that combine traditional electrophysiology techniques with optogenetics.
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Pollnow, Stefan. Characterizing Cardiac Electrophysiology during Radiofrequency Ablation: An Integrative Ex vivo, In silico, and In vivo Approach. KIT Scientific Publishing, 2019.

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Book chapters on the topic "In vivo Electrophysiology"

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Zhou, Yi, He Li, and Zhongju Xiao. "In Vivo Patch-Clamp Studies." In Patch Clamp Electrophysiology, 259–71. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_13.

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Coddington, Luke T., and Joshua T. Dudman. "In Vivo Optogenetics with Stimulus Calibration." In Patch Clamp Electrophysiology, 273–83. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_14.

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Leng, Gareth, and Nancy Sabatier. "Electrophysiology of Magnocellular Neuronsin Vivo." In Neurophysiology of Neuroendocrine Neurons, 1–28. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118606803.ch1.

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von Dincklage, F., and B. Rehberg. "In-vivo Electrophysiology of Anesthetic Action." In Sleep and Anesthesia, 243–55. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4614-0173-5_11.

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Zhu, Xiyu, and Anthony A. Grace. "Technical Considerations for In Vivo Electrophysiology." In Neuromethods, 275–85. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2589-7_24.

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Stanfa, Louise C., and Anthony H. Dickenson. "In Vivo Electrophysiology of Dorsal-Horn Neurons." In Pain Research, 139–53. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1007/978-1-59259-770-3_12.

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Simonnet, Jean, Louis Richevaux, and Desdemona Fricker. "Single or Double Patch-Clamp Recordings In Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics." In Patch Clamp Electrophysiology, 285–309. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_15.

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Alba, Laura A., Elizabeth Baker, and Katherine K. M. Stavropoulos. "In Vivo Electrophysiology for Reward Anticipation and Processing." In The Brain Reward System, 307–26. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1146-3_15.

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Bastian, Chinthasagar, Sylvain Brunet, and Selva Baltan. "Ex Vivo Studies of Optic Nerve Axon Electrophysiology." In Methods in Molecular Biology, 169–77. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0585-1_13.

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Roberts, M. H. T., and M. Davies. "In vivo Electrophysiology of Receptors Mediating the Central Nervous System Actions of 5-Hydroxytryptamine." In Serotonin, 70–76. London: Palgrave Macmillan UK, 1989. http://dx.doi.org/10.1007/978-1-349-10114-6_9.

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Conference papers on the topic "In vivo Electrophysiology"

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Chen, Fu-Der, Hannes Wahn, Tianyuan Xue, Youngho Jung, John N. Straguzzi, Saeed S. Azadeh, Andrei Stalmashonak, et al. "Implantable Neural Probe System for Patterned Photostimulation and Electrophysiology Recording." In CLEO: Applications and Technology. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/cleo_at.2022.jth6a.7.

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We demonstrate a system for implantable nanophotonic neural probes with custom packaging and peripherals. The probes, which were manufactured on 200mm Si wafers, monolithically integrate SiN waveguides with TiN electrophysiology electrodes and were tested in vivo.
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Pisoni, M., Y. Goulam Houssen, B. Mathieu, P. Bizouard, S. Dieudonné, and B. Bathellier. "Towards two-photon all-optical electrophysiology with acousto-optic scanning." In Optics and the Brain. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/brain.2024.bs3c.6.

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We explored ultrafast local volume excitation method for multiple neurons investigation. By employing low repetition rate femtosecond lasers and an AOD-based microscope, we achieved precise spatial and temporal photostimulation of neuron ensembles in vivo.
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Gong, Yan, Liu Xiang, and Wen Li. "A FLEXIBLE ORIGAMI OPTO-ELECTRO ARRAY FOR IN VIVO OPTOGENETIC STIMULATION AND ELECTROPHYSIOLOGY RECORDINGS FROM DORSAL ROOT GANGLION." In 2022 Solid-State, Actuators, and Microsystems Workshop. San Diego: Transducer Research Foundation, 2022. http://dx.doi.org/10.31438/trf.hh2022.40.

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Wang, Yi, Sung-Ho Lee, Yen-Yu Ian Shih, and Yuan-Shin Lee. "Design and Fabrication of MRI-Compatible and Flexible Neural Microprobes for Deep Brain Stimulation and Neurological Treatment Applications." In ASME 2022 17th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/msec2022-85832.

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Abstract This paper presents a new design and fabrication method of flexible neural microprobes for deep brain stimulations (DBS) and neurological treatment applications. The developed new flexible microprobes are compatible with functional magnetic resonance imaging (fMRI) and can be used for neurological studies of brain functions under functional imaging such as fMRI for a long period of time. In this paper, the materials of the flexible neural microprobes are comprehensively selected to minimize the magnetic resonance imaging (MRI) artifacts, which limits many conventional feasible manufacturing processes to be used. Polyimide was adopted for the substrate of the neural microprobe, which has good biocompatibility and a relatively lower Young’s modulus. A 200 nm chromium reinforcement layer was embedded in the microprobe to attenuate its implantation stiffness while remaining flexible. Gold electroplating was employed to modify the electrode sites to improve the signal quality and sensitivity. The designed new neural microprobes were successfully fabricated at our NCSU Nanofabrication Facility (NNF) and bonded to a customized PCB. After the fabrication, the developed neural microprobes were characterized to validate their effectiveness. In vivo simultaneous DBS-fMRI experiments were conducted in surgery rooms on a rat’s deep brain targets, demonstrating the potential applications of our neural microprobe for neuroscience studies, medical diagnosis, and treatment applications. In vivo electrophysiology results show the effectiveness of our fabricated neural microprobes.
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Reports on the topic "In vivo Electrophysiology"

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Cao, Siyang, Yihao Wei, Tiantian Qi, Peng Liu, Yingqi Chen, Fei Yu, Hui Zeng, and Jian Weng. Stem cell therapy for peripheral nerve injury: An up-to-date meta-analysis of 55 preclinical researches. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, October 2022. http://dx.doi.org/10.37766/inplasy2022.10.0083.

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Review question / Objective: It has been the gold standard for decades to reconstruct a large peripheral nerve injury with a nerve autograft, and this remains true today as well. In addition to nerve autografts, biological conduits and vessels can also be applied. A fair amount of studies have examined the benefits of adding stem cells to the lumen of a nerve conduit. The aim of this meta-analysis was to summarize animal experiments related to the utilization of stem cells as a luminal additive when rebuilding a peripheral nerve injury using nerve grafts. Eligibility criteria: The inclusion criteria were as following: 1.Reconstruction of peripheral nerve injury; 2.Complete nerve transection with gap defect created; 3.Animal in-vivo models; 4.Experimental comparisons between nerve conduits containing and not containing one type of stem cell; 5.Functional testing and electrophysiology evaluations are performed. The exclusion criteria were as following: 1.Repair of central nervous system; 2.Nerve repair is accomplished by end-to-end anastomosis; 3.Animal models of entrapment injuries, frostbite, traction injuries and electric injuries; 4.Nerve conduits made from autologous epineurium; 5.Clinical trials, reviews, letters, conference papers, meta-analyses or commentaries; 6.Same studies have been published in different journals under the same or a different title.
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