Relevant bibliographies by topics / In vivo electrophysiology recording

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'In vivo electrophysiology recording'

Author: Grafiati
Published: 4 June 2021
Last updated: 12 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'In vivo electrophysiology recording.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "In vivo electrophysiology recording"

1

Nelson, Matthew J., Silvana Valtcheva, and Laurent Venance. "Magnitude and behavior of cross-talk effects in multichannel electrophysiology experiments." Journal of Neurophysiology 118, no. 1 (July 1, 2017): 574–94. http://dx.doi.org/10.1152/jn.00877.2016.

Full text
Abstract:
Modern neurophysiological experiments frequently involve multiple channels separated by very small distances. A unique methodological concern for multiple-electrode experiments is that of capacitive coupling (cross-talk) between channels. Yet the nature of the cross-talk recording circuit is not well known in the field, and the extent to which it practically affects neurophysiology experiments has never been fully investigated. Here we describe a simple electrical circuit model of simultaneous recording and stimulation with two or more channels and experimentally verify the model using ex vivo brain slice and in vivo whole-brain preparations. In agreement with the model, we find that cross-talk amplitudes increase nearly linearly with the impedance of a recording electrode and are larger for higher frequencies. We demonstrate cross-talk contamination of action potential waveforms from intracellular to extracellular channels, which is observable in part because of the different orders of magnitude between the channels. This contamination is electrode impedance-dependent and matches predictions from the model. We use recently published parameters to simulate cross-talk in high-density multichannel extracellular recordings. Cross-talk effectively spatially smooths current source density (CSD) estimates in these recordings and induces artefactual phase shifts where underlying voltage gradients occur; however, these effects are modest. We show that the effects of cross-talk are unlikely to affect most conclusions inferred from neurophysiology experiments when both originating and receiving electrode record signals of similar magnitudes. We discuss other types of experiments and analyses that may be susceptible to cross-talk, techniques for detecting and experimentally reducing cross-talk, and implications for high-density probe design. NEW & NOTEWORTHY We develop and experimentally verify an electrical circuit model describing cross-talk that necessarily occurs between two channels. Recorded cross-talk increased with electrode impedance and signal frequency. We recorded cross-talk contamination of spike waveforms from intracellular to extracellular channels. We simulated high-density multichannel extracellular recordings and demonstrate spatial smoothing and phase shifts that cross-talk enacts on CSD measurements. However, when channels record similar-magnitude signals, effects are modest and unlikely to affect most conclusions.
APA, Harvard, Vancouver, ISO, and other styles
2

Morizio, James, and Vinson Go. "Proceedings #30: Implantable Neural Recording and Stimulation Technologies for in vivo Electrophysiology." Brain Stimulation 12, no. 2 (March 2019): e96-e97. http://dx.doi.org/10.1016/j.brs.2018.12.199.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Noguchi, Asako, Yuji Ikegaya, and Nobuyoshi Matsumoto. "In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives." Sensors 21, no. 4 (February 19, 2021): 1448. http://dx.doi.org/10.3390/s21041448.

Full text
Abstract:
Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp recording techniques. These techniques enable us to record membrane potentials (including action potentials) of individual neurons of not only anesthetized but also actively behaving animals. This whole-cell recording method enables us to reveal how neuronal activities support brain function at the single-cell level. In this review, we introduce previous studies using in vivo patch-clamp recording techniques and recent findings primarily regarding neuronal activities in the hippocampus for behavioral function. We further discuss how we can bridge the gap between electrophysiology and biochemistry.
APA, Harvard, Vancouver, ISO, and other styles
4

Hong, Guosong, Tian-Ming Fu, Mu Qiao, Robert D. Viveros, Xiao Yang, Tao Zhou, Jung Min Lee, Hong-Gyu Park, Joshua R. Sanes, and Charles M. Lieber. "A method for single-neuron chronic recording from the retina in awake mice." Science 360, no. 6396 (June 28, 2018): 1447–51. http://dx.doi.org/10.1126/science.aas9160.

Full text
Abstract:
The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.
APA, Harvard, Vancouver, ISO, and other styles
5

Fiáth, Richárd, Patrícia Beregszászi, Domonkos Horváth, Lucia Wittner, Arno A. A. Aarts, Patrick Ruther, Hercules P. Neves, Hajnalka Bokor, László Acsády, and István Ulbert. "Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe." Journal of Neurophysiology 116, no. 5 (November 1, 2016): 2312–30. http://dx.doi.org/10.1152/jn.00318.2016.

Full text
Abstract:
Recording simultaneous activity of a large number of neurons in distributed neuronal networks is crucial to understand higher order brain functions. We demonstrate the in vivo performance of a recently developed electrophysiological recording system comprising a two-dimensional, multi-shank, high-density silicon probe with integrated complementary metal-oxide semiconductor electronics. The system implements the concept of electronic depth control (EDC), which enables the electronic selection of a limited number of recording sites on each of the probe shafts. This innovative feature of the system permits simultaneous recording of local field potentials (LFP) and single- and multiple-unit activity (SUA and MUA, respectively) from multiple brain sites with high quality and without the actual physical movement of the probe. To evaluate the in vivo recording capabilities of the EDC probe, we recorded LFP, MUA, and SUA in acute experiments from cortical and thalamic brain areas of anesthetized rats and mice. The advantages of large-scale recording with the EDC probe are illustrated by investigating the spatiotemporal dynamics of pharmacologically induced thalamocortical slow-wave activity in rats and by the two-dimensional tonotopic mapping of the auditory thalamus. In mice, spatial distribution of thalamic responses to optogenetic stimulation of the neocortex was examined. Utilizing the benefits of the EDC system may result in a higher yield of useful data from a single experiment compared with traditional passive multielectrode arrays, and thus in the reduction of animals needed for a research study.
APA, Harvard, Vancouver, ISO, and other styles
6

Holst, Gregory L., William Stoy, Bo Yang, Ilya Kolb, Suhasa B. Kodandaramaiah, Lu Li, Ulf Knoblich, et al. "Autonomous patch-clamp robot for functional characterization of neurons in vivo: development and application to mouse visual cortex." Journal of Neurophysiology 121, no. 6 (June 1, 2019): 2341–57. http://dx.doi.org/10.1152/jn.00738.2018.

Full text
Abstract:
Patch clamping is the gold standard measurement technique for cell-type characterization in vivo, but it has low throughput, is difficult to scale, and requires highly skilled operation. We developed an autonomous robot that can acquire multiple consecutive patch-clamp recordings in vivo. In practice, 40 pipettes loaded into a carousel are sequentially filled and inserted into the brain, localized to a cell, used for patch clamping, and disposed. Automated visual stimulation and electrophysiology software enables functional cell-type classification of whole cell-patched cells, as we show for 37 cells in the anesthetized mouse in visual cortex (V1) layer 5. We achieved 9% yield, with 5.3 min per attempt over hundreds of trials. The highly variable and low-yield nature of in vivo patch-clamp recordings will benefit from such a standardized, automated, quantitative approach, allowing development of optimal algorithms and enabling scaling required for large-scale studies and integration with complementary techniques. NEW & NOTEWORTHY In vivo patch-clamp is the gold standard for intracellular recordings, but it is a very manual and highly skilled technique. The robot in this work demonstrates the most automated in vivo patch-clamp experiment to date, by enabling production of multiple, serial intracellular recordings without human intervention. The robot automates pipette filling, wire threading, pipette positioning, neuron hunting, break-in, delivering sensory stimulus, and recording quality control, enabling in vivo cell-type characterization.
APA, Harvard, Vancouver, ISO, and other styles
7

Wei, Wen Jing, Yi Lin Song, Wen Tao Shi, Chun Xiu Liu, Ting Jun Jiang, and Xin Xia Cai. "A Novel Microelectrode Array Probe Integrated with Electrophysiology Reference Electrode for Neural Recording." Key Engineering Materials 562-565 (July 2013): 67–73. http://dx.doi.org/10.4028/www.scientific.net/kem.562-565.67.

Full text
Abstract:
Nowadays, the study of brain function is advanced by implantable microelectrode arrays for they can simultaneously record signals from different groups of neurons regarding complex neural processes. This article presents the fabrication, characterization and use in vivo neural recording of an implantable microelectrode array probe which integrated with electrophysiology reference electrode. The probe was implemented on Silicon-On-Insulator (SOI) wafer using Micro-Electro-Mechanical-Systems (MEMS) methods, so the recording-site configurations and high-density electrode placement could be precisely defined. The 16 recording sites and the reference electrode were made of platinum. Double layers of platinum electrodes were used so that the width of the reference electrode was as small as 6 μm. The average impedance of the microelectrodes was 0.13 MΩ at 1 kHz. The probe has been employed to record the neural signals of rat, and the results showed that the signal-to-noise ratio (SNR) of the novel probe was as high as 10 and the ordinary probe was 3. Among the 16 recording sites, there are 9 effective sites having recorded useful signals for the probe with reference electrode and 6 for the ordinary probe.
APA, Harvard, Vancouver, ISO, and other styles
8

Lee, Peter, Jorge G. Quintanilla, José M. Alfonso-Almazán, Carlos Galán-Arriola, Ping Yan, Javier Sánchez-González, Nicasio Pérez-Castellano, et al. "In vivo ratiometric optical mapping enables high-resolution cardiac electrophysiology in pig models." Cardiovascular Research 115, no. 11 (February 7, 2019): 1659–71. http://dx.doi.org/10.1093/cvr/cvz039.

Full text
Abstract:
Abstract Aims Cardiac optical mapping is the gold standard for measuring complex electrophysiology in ex vivo heart preparations. However, new methods for optical mapping in vivo have been elusive. We aimed at developing and validating an experimental method for performing in vivo cardiac optical mapping in pig models. Methods and results First, we characterized ex vivo the excitation-ratiometric properties during pacing and ventricular fibrillation (VF) of two near-infrared voltage-sensitive dyes (di-4-ANBDQBS/di-4-ANEQ(F)PTEA) optimized for imaging blood-perfused tissue (n = 7). Then, optical-fibre recordings in Langendorff-perfused hearts demonstrated that ratiometry permits the recording of optical action potentials (APs) with minimal motion artefacts during contraction (n = 7). Ratiometric optical mapping ex vivo also showed that optical AP duration (APD) and conduction velocity (CV) measurements can be accurately obtained to test drug effects. Secondly, we developed a percutaneous dye-loading protocol in vivo to perform high-resolution ratiometric optical mapping of VF dynamics (motion minimal) using a high-speed camera system positioned above the epicardial surface of the exposed heart (n = 11). During pacing (motion substantial) we recorded ratiometric optical signals and activation via a 2D fibre array in contact with the epicardial surface (n = 7). Optical APs in vivo under general anaesthesia showed significantly faster CV [120 (63–138) cm/s vs. 51 (41–64) cm/s; P = 0.032] and a statistical trend to longer APD90 [242 (217–254) ms vs. 192 (182–233) ms; P = 0.095] compared with ex vivo measurements in the contracting heart. The average rate of signal-to-noise ratio (SNR) decay of di-4-ANEQ(F)PTEA in vivo was 0.0671 ± 0.0090 min−1. However, reloading with di-4-ANEQ(F)PTEA fully recovered the initial SNR. Finally, toxicity studies (n = 12) showed that coronary dye injection did not generate systemic nor cardiac damage, although di-4-ANBDQBS injection induced transient hypotension, which was not observed with di-4-ANEQ(F)PTEA. Conclusions In vivo optical mapping using voltage ratiometry of near-infrared dyes enables high-resolution cardiac electrophysiology in translational pig models.
APA, Harvard, Vancouver, ISO, and other styles
9

Eckardt, Lars, Andreas Meißner, Paulus Kirchhof, Thomas Weber, Martin Borggrefe, Günter Breithardt, Hugo Van Aken, and Wilhelm Haverkamp. "In vivo recording of monophasic action potentials in awake dogs - new applications for experimental electrophysiology." Basic Research in Cardiology 96, no. 2 (March 1, 2001): 169–74. http://dx.doi.org/10.1007/s003950170067.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Jeon, Saeyeong, Youjin Lee, Daeho Ryu, Yoon Kyung Cho, Yena Lee, Sang Beom Jun, and Chang-Hyeon Ji. "Implantable Optrode Array for Optogenetic Modulation and Electrical Neural Recording." Micromachines 12, no. 6 (June 19, 2021): 725. http://dx.doi.org/10.3390/mi12060725.

Full text
Abstract:
During the last decade, optogenetics has become an essential tool for neuroscience research due to its unrivaled feature of cell-type-specific neuromodulation. There have been several technological advances in light delivery devices. Among them, the combination of optogenetics and electrophysiology provides an opportunity for facilitating optogenetic approaches. In this study, a novel design of an optrode array was proposed for realizing optical modulation and electrophysiological recording. A 4 × 4 optrode array and five-channel recording electrodes were assembled as a disposable part, while a reusable part comprised an LED (light-emitting diode) source and a power line. After the characterization of the intensity of the light delivered at the fiber tips, in vivo animal experiment was performed with transgenic mice expressing channelrhodopsin, showing the effectiveness of optical activation and neural recording.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "In vivo electrophysiology recording"

1

Annecchino, Luca. "Development and validation of a robotic two-photon targeted whole-cell recording system for in vivo electrophysiology." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/56991.

Full text
Abstract:
Understanding the functional principles of the mammalian cortical circuit is a major challenge in neuroscience. To make progress towards this understanding, one needs to be able to assess the behavioural dynamics of individual neuronal elements of this circuit. Manual whole-cell recording (WCR) in vivo is recognised as the “gold standard” method for electrophysiological interrogation of individual neurons. It allows subthreshold and suprathreshold signals to be recorded, perturbations to be applied through current injection, and DNA vectors to be directly delivered into the patched cells as part of the pipette internal solution. Unfortunately, the WCR technique for in vivo application is a “blind” procedure and has a low-throughput. In addition, the genetic and morphological identity of the recorded neuron often cannot be accounted for. The inherent cell-type non selectivity of this technique can be overcome by combining WCR with two-photon laser scanning microscopy, and targeting recordings to specifically labelled individual cells or cell classes. Targeted electrophysiological interrogation allows one to examine the properties of both single cells and neuronal assemblies and, additionally, the role of cell type-specific proteins in orchestrating neuronal responses. Targeted recordings in vivo may enable to test a wide range of hypotheses related to information processing in the cortical circuits. However, probing and studying the properties of individual cells in live animal preparations remains a challenge in neuroscience. In particular, precise vision-guided control of patch pipette motion and viewpoint generation of microscope objective for targeted single-cell electrophysiological interrogation is problematic. It requires specialised skills acquired through extensive practice and training by individual operators. Although automatic patch clamp technology has been in use for some years exclusively for cell culture-based paradigms, only recently has Kodandaramaiah et al. demonstrated a “blind” automated patch clamp system for in vivo recordings. However, a fully automatic method for in vivo WCR targeting specific cells or cell-types has not been implemented in any robotic system so far. In this study an automated two-photon targeted whole-cell patch clamping algorithm is demonstrated as a workable solution. The aim of this work was to develop a robotic integrated targeted autopatcher that minimised labour intensive procedures and increased the throughput both in blind and two-photon targeted WCR in live animals. The system automatically controls a micromanipulator, a microelectrode signal amplifier a two-photon microscope and a custom made regulator for controlling the internal pressure of the pipette. The two-photon microscope acquires images of fluorescently labelled cells, and cell-targets for patch clamp are selected via a point-and-click graphical user interface. Optical coordinates are initially converted to the micromanipulator coordinate system and a suitable path calculated to guide the patch pipette towards the target. This platform allows to compensate for brain tissue deformation and subsequent neuronal target movement caused by pipette insertion. As proof-of-concept, the system was tested in both “blind” and two-photon targeted paradigms and achieved performances comparable to human operators, in terms of yield, recording quality and operational speed. Hit rate for “blind” WCR was 51.4% (n=18, 35 attempts across 5 mice). RITA was also calibrated and tested for targeting specific cell types in the cortex of intact mouse brain labelled via fluorescent dye loading (e.g. Oregon Green BAPTA-1 and/or Sulforhodamine 101). Pipettes were automatically guided to the target cells and recordings obtained from visually identified neurons as well as astrocytes. These results prove the feasibility of robotic targeted WCR patch clamp in vivo and establish this system as a powerful tool for automated electrophysiological experiments in the brain.
APA, Harvard, Vancouver, ISO, and other styles
2

Lau, Petrina Yau Pok. "Long-term plasticity of excitatory inputs onto identified hippocampal neurons in the anaesthetized rat." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:172e0d36-0d67-4932-962e-9ee08dcc366c.

Full text
Abstract:
Use-dependent long-term plasticity in synaptic connections represents the cellular substrate for learning and memory. The hippocampus is the most thoroughly investigated brain area for long-term synaptic plasticity, long-term potentiation (LTP) and long-term depression (LTD) are both well characterized in glutamatergic excitatory connections between hippocampal principal cells in vitro and in vivo. An increasing number of studies based on acute brain slice preparations report LTP and LTD in excitatory synapses onto postsynaptic hippocampal GABAergic inhibitory interneurons. However, a systematic study of activity-induced long-term plasticity in excitatory synaptic connections to inhibitory GABAergic interneurons in vivo is missing. To determine whether LTP and LTD occur in excitatory synaptic connections to the hippocampal CA1 area GABAergic interneurons types in intact brain, I have used juxtacellular recording to measure synaptically evoked short-delay postsynaptic action potential probability in identified CA1 neurons in the urethane-anaesthetized rats. Plasticity in excitatory synaptic connections to CA1 cell types was measured as a change of afferent pathway stimulation-evoked postsynaptic spike probability and delay. In the study only experiments with monosynaptic-like short-delay (range 3-12 ms) postsynaptic spikes phase-locked to afferent stimulation were used. Afferent fibres were stimulated from the CA1 area of the hippocampus at the contralateral (left) side to avoid simultaneous monosynaptic activation of GABAergic fibres and to exclude antidromic spikes in recorded CA1 cells (in right hemisphere). Plasticity in pathways was tested using theta-burst high-frequency stimulation (TBS, 100 pulses), which is one of the most common synaptic plasticity induction protocols in acute brain slice studies. I discovered that TBS elicited permanent potentiation in single shock-evoked postsynaptic spike probability with shortening or no change in evoked spike latency in various postsynaptic neuron types including three identified pyramidal cells and parvalbumin-expressing (PV+) interneurons. Most fast-spiking PV+ cells showed LTP including an axo-axonic cell and one bistratified cell, whereas two identified basket cells exhibited LTD in similar experimental conditions. In addition, I discovered diverse plasticity in non-fast spiking interneurons, reporting LTP in an ivy cell, and LTD in three incompletely identified regular-spiking CA1 interneurons. I report that the underlying brain state, defined as theta oscillation (3-6 Hz) or non-theta in local field potential, failed to explain whether LTP, LTD or no plasticity was generated in interneurons. The results show that activity-induced potentiation and depression similar to LTP and LTD also occur in excitatory synaptic pathways to various CA1 interneurons types in vivo. I propose that long-term plasticity in excitatory connections to inhibitory interneurons may be take place in learning and memory processes in the hippocampus.
APA, Harvard, Vancouver, ISO, and other styles
3

Chaudun, Fabrice. "Involvement of dorsomedial prefrontal projections pathways to the basolateral amygdala and ventrolateral periaqueductal grey matter in conditioned fear expression." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0118/document.

Full text
Abstract:
A l’heure actuelle, une des principales questions des neurosciences comportementales est de comprendre les bases neurales des apprentissages et de comprendre comment des modifications au sein de circuits neuronaux spécifiques contrôlent les changements comportementaux liés à une expérience particulière. De nombreuses études ont récemment mis en évidence le rôle important des circuits neuronaux dans les phénomènes d’apprentissages associatifs, et notamment dans la régulation des comportements de peur. Cependant, leurs caractéristiques anatomiques et fonctionnelles restent encore largement inconnues. L’une des principales fonctions des circuits neuronaux est leur capacité à adapter le comportement en fonction de la nature des informations internes ou environnementales disponibles. Malgré de nombreux progrès réalisés sur la compréhension des substrats et mécanismes neuronaux sous tendant le conditionnement de peur au sein de structures telles que l'amygdale (AMG), le cortex préfrontal dorso-médian (dmPFC) et la substance grise periaqueducale (PAG), les mécanismes neuronaux gouvernant les interactions inter-structure ainsi que le contrôle local de ces différents circuits neuronaux restent encore largement inconnus. Dans ce contexte, ce travail de thèse a eupour objectifs principaux, d’évaluer la contribution des voies de projections dmPFC-BLA et dmPFC-vlPAG dans la régulation des comportements de peur, et, d’identifier les mécanismes neuronaux sous-jacent contrôlant l'expression de la peur. Afin de répondre à ces questions, nous avons utilisé conjointement des enregistrements électrophysiologiques unitaires et de potentiels de champs couplés à des approches optogénétiques au cours de l’expression de la peur conditionnée. Nous avons pu mettre en évidence un nouveau mécanisme neuronal basé sur une oscillation cérébrale à 4 Hz entre le dmPFC et le BLA impliqué dans la synchronisation neuronale des neurones de ces deux structures nécessaire à l’expression de la peur. Nous avons aussi démontré que le dmPFC via ses projections sur le vlPAG contrôle directement l’expression de la peur. Ensemble, nos données contribuent à une meilleure compréhension des circuits neuronaux ainsi que des mécanismes du comportement de peur qui dans le futur pourront aider à une amélioration thérapeutique des troubles anxieux
A central endeavour of modern neuroscience is to understand the neural basis of learningand how the selection of dedicated circuits modulates experience-dependent changes inbehaviour. Decades of research allowed a global understanding of the computations occurring inhard-wired networks during associative learning, in particular fear behaviour. However, brainfunctions are not only derived from hard-wired circuits, but also depend on modulation of circuitfunction. It is therefore realistic to consider that brain areas contain multiple potential circuitswhich selection is based on environmental context and internal state. Whereas the role of entirebrain areas such as the amygdala (AMG), the dorsal medial prefrontal cortex (dmPFC) or theperiaqueductal grey matter (PAG) in fear behaviour is reasonably well understood at themolecular and synaptic levels, there is a big gap in our knowledge of how fear behaviour iscontrolled at the level of defined circuits within these brain areas. More particularly, whereas thedmPFC densely project to both the basolateral amygdala (BLA) and PAG, the contributions ofthese two projections pathway during fear behaviour are largely unknown. Beside theinvolvement of these neuronal pathways in the transmission of fear related-information, theneuronal mechanisms involved in the encoding of fear behaviour within these pathways are alsovirtually unknown. In this context, the present thesis work had two main objectives. First,evaluate the contribution of the dmPFC-BLA and dmPFC-vlPAG pathways in the regulation offear behaviour, and second, identify the neuronal mechanisms controlling fear expression in thesecircuits. To achieve these goals, we used a combination of single unit and local field potentialrecordings coupled to optogenetic approaches in behaving animals submitted to a discriminativefear conditioning paradigm. Our results first, identified a novel neuronal mechanism of fear expression based on the development of 4 H oscillations within dmPFC-BLA circuits thatdetermine the dynamics of freezing behaviour and allows the long-range synchronization offiring activities to drive fear behaviour. Secondly, our results identified the precise circuitry at thelevel of the dmPFC and vlPAG that causally regulate fear behaviour. Together these data provideimportant insights into the neuronal circuits and mechanisms of fear behaviour. Ultimately thesefindings will eventually lead to a refinement of actual therapeutic strategies for pathological conditions such as anxiety disorders
APA, Harvard, Vancouver, ISO, and other styles
4

Pye, Richard Laurence. "Measuring the Acute Physiological Effects of Leptin in the Carotid Body." Wright State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=wright1449583350.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Bernstein, Jacob (Jacob Gold). "Development of extracellular electrophysiology methods for scalable neural recording." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/107581.

Full text
Abstract:
Thesis: Ph. D., Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2016.
Cataloged from PDF version of thesis.
Includes bibliographical references.
In order to map the dynamics of neural circuits in mammalian brains, there is a need for tools that can record activity over large volumes of tissue and correctly attribute the recorded signals to the individual neurons that generated them. High-resolution neural activity maps will be critical for the discovery of new principles of neural coding and neural computation, and to test computational models of neural circuits. Extracellular electrophysiology is a neural recording method that has been developed to record from large populations of neurons, but well-known problems with signal attribution pose an existential threat to the viability of further system scaling, as analyses of network function become more sensitive to errors in attribution. A key insight is that blind-source separation algorithms such as Independent Component Analysis may ameliorate problems with signal attribution. These algorithms require recording signals at much finer spatial resolutions than existing probes have accomplished, which places demands on recording system bandwidth. We present several advances to technologies in neural recording systems, and a complete neural recording system designed to investigate the challenges of scaling electrophysiology to whole brain recording. We have developed close-packed microelectrode arrays with the highest density of recording sites yet achieved, for which we built our own data acquisition hardware, developed with a computational architecture specifically designed to scale to over several orders of magnitude. We also present results from validation experiments using colocalized patch clamp recording to obtain ground-truth activity data. This dataset provides immediate insight into the nature of electrophysiological signals and the interpretation of data collected from any electrophysiology recording system. This data is also essential in order to optimize probe development and data analysis algorithms which will one day enable whole-brain activity mapping.
by Jacob G. Bernstein.
Ph. D.
APA, Harvard, Vancouver, ISO, and other styles
6

Silpa, Nagari. "NANOSTRUCTURED SENSORS FOR IN-VIVO NEUROCHEMICAL RECORDING." UKnowledge, 2007. http://uknowledge.uky.edu/gradschool_theses/487.

Full text
Abstract:
L-glutamate plays a vital role in central nervous system. It is a neurotransmitterassociated with several neurological disorders like Parkinson's disease, epilepsyand stroke. Continuous and fast monitoring of this neurotransmitter has become amajor concern for neuroscientists throughout the world. A simple, sensitive, and reliable L-glutamate microsensor with short responsetime has been developed using ceramic-based microelectrode arrays with platinum recording sites. The electrodes were modified by electrodeposition of Platinum black (Pt-black) to detect hydrogen peroxide (H2O2) which was produced by enzymatic reactions of glutamate oxidase immobilized on the electrode surface. Modification of Pt electrodes with Pt-black has been adoptedbecause the microscale roughness of Pt-black increases the effective surface area of the electrode and promotes efficiency of H2O2 electro-oxidation. The modified Pt recording sites were coated with m-phenylenediamine (mPD) and L-glutamate oxidase (L-GluOx). mPD acts as an barrier for extracellular interferents such as ascorbic acid and dopamine, thus increasing the selectivity of electrode for Glutamate (Glu). This modified microsensor was highly sensitive to H2O2(686.3??156.48 ??AmM-1cm-2), and Glutamate (492.2??112.67 ??AmM-1cm-2) at 700mV versus Ag/AgCl reference. Deposition of Pt nano-particles on recording sites enhanced the sensitivity to H2O2 by 2 times and the sensitivity to glutamate by 1.5 times.
APA, Harvard, Vancouver, ISO, and other styles
7

Nagari, Silpa. "Nano-structured sensors for in-vivo neurochemical recording." Lexington, Ky. : [University of Kentucky Libraries], 2007. http://hdl.handle.net/10225/735.

Full text
Abstract:
Thesis (M.S.)--University of Kentucky, 2007.
Title from document title page (viewed on March 24, 2008). Document formatted into pages; contains: ix, 55 p. : ill. (some col.). Includes abstract and vita. Includes bibliographical references (p. 53-54).
APA, Harvard, Vancouver, ISO, and other styles
8

Dodds, Catherine Jane. "The action of naturally-occuring semiochemicals on feeding behaviour and neurophysiology of the field slug Deroceras reticulatum (Mueller)." Thesis, University of Portsmouth, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310443.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Hasegawa, Taku. "A wireless system with a motorized microdrive for neural recording in freely behaving animals." 京都大学 (Kyoto University), 2015. http://hdl.handle.net/2433/199467.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Kodandaramaiah, Suhasa Bangalore. "Robotics for in vivo whole cell patch clamping." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/51932.

Full text
Abstract:
Whole-cell patch clamp electrophysiology of neurons in vivo enables the recording of electrical events in cells with great precision, and supports a wide diversity of morphological and molecular analysis experiments important for the understanding of single-cell and network functions in the intact brain. However, high levels of skill are required in order to perform in vivo patching, and the process is time-consuming and painstaking. Robotic systems for in vivo patching would not only empower a great number of neuroscientists to perform such experiments, but would also open up fundamentally new kinds of experiment enabled by the resultant high throughput and scalability. We discovered that in vivo blind whole cell patch clamp electrophysiology could be implemented as a straightforward algorithm and developed an automated robotic system that was capable of performing this algorithm. We validated the performance of the robot in both the cortex and hippocampus of anesthetized mice. The robot achieves yields, cell recording qualities, and operational speeds that are comparable to, or exceed, those of experienced human investigators. Building upon this framework, we developed a multichannel version of “autopatcher” robot capable establishing whole cell patch clamp recordings from pairs and triplets of neurons in the cortex simultaneously. These algorithms can be generalized to control arbitrarily large number of electrodes and the high yield, throughput and automation of complex set of tasks results in a practical solution for conducting patch clamp recordings in potentially dozens of interconnected neurons in vivo.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "In vivo electrophysiology recording"

1

Improved nerve signal recording: Methods and analogue circuits. Konstanz: Hartung-Gorre, 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

1988), Freiburg Focus on Biomeasurement (4th. Electrodes for stimulation and bioelectric potential recording: 4th Freisburg Focus on Biomeasurement, Februar [sic] 22nd and 23rd, 1988. March: Biomesstechnik-Verlag March GmbH, 1988.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Ferster, David. Patch Clamp Recording in Vivo. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0002.

Full text
Abstract:
Patch clamp recording in vivo allows an investigator to study intracellular membrane potentials in an intact organism (as opposed to cells in culture or acute brain slices). This technique is a reliable method of obtaining high-quality intracellular recordings from neurons, regardless of their size, in several parts of the mammalian brain. This chapter will describe the principles and practice of performing patch clamp experiments in vivo, beginning with a brief history of the technological developments that have made this technique possible.
APA, Harvard, Vancouver, ISO, and other styles
4

Electrophysiological Recording Techniques. Humana Press, 2010.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Tseng, Hua-an, Richie E. Kohman, and Xue Han. Optogenetics and Electrophysiology. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0009.

Full text
Abstract:
Throughout the history of neuroscience, electrophysiological and imaging techniques have been utilized to observe neural signals at various spatial and temporal scales. However, it has been difficult to manipulate the activity of specific cells or neural circuits with the spatial and temporal resolutions relevant to neural coding. A novel technique called optogenetics, has recently been developed to control the activity of specific cells. This technique allows rapid and reversible optical activation or silencing of specific cells, which have been genetically transduced with light-sensitive molecules. The development of microbial opsin-based optogenetic molecular sensors has made optogenetics easily adaptable in various in vivo and in vitro preparations, and the technique has already been applied to understand neural circuit mechanisms of many behaviors and diseases. Here, we provide an introduction to optogenetics, the practical concerns in using the technique in vivo, and examples of applications that combine traditional electrophysiology techniques with optogenetics.
APA, Harvard, Vancouver, ISO, and other styles
6

(Editor), Koki Shimoji, and William D. Jr. Willis (Editor), eds. Evoked Spinal Cord Potentials: An illustrated Guide to Physiology, Pharmocology, and Recording Techniques. Springer, 2006.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

1935-, Shimoji Kōki, and Willis William D. 1934-, eds. Evoked spinal cord potentials: An illustrated guide to physiology, pharmacology, and recording techniques. Tokoyo: Springer, 2006.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

1935-, Shimoji Kōki, and Willis William D. 1934-, eds. Evoked spinal cord potentials: An illustrated guide to physiology, pharmacology, and recording techniques. Tokoyo: Springer, 2006.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

Campagnola, Luke, and Paul Manis. Patch Clamp Recording in Brain Slices. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0001.

Full text
Abstract:
Patch clamp recording in brain slices allows unparalleled access to neuronal membrane signals in a system that approximates the in-vivo neural substrate while affording greater control of experimental conditions. In this chapter we discuss the theory, methodology, and practical considerations of such experiments including the initial setup, techniques for preparing and handling viable brain slices, and patching and recording signals. A number of practical and technical issues faced by electrophysiologists are also considered, including maintaining slice viability, visualizing and identifying healthy cells, acquiring reliable patch seals, amplifier compensation features, hardware configuration, sources of electrical noise and table vibration, as well as basic data analysis issues and some troubleshooting tips.
APA, Harvard, Vancouver, ISO, and other styles
10

Coleman, William L., and R. Michael Burger. Extracellular Single-Unit Recording and Neuropharmacological Methods. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0003.

Full text
Abstract:
Small biogenic changes in voltage such as action potentials in neurons can be monitored using extracellular single unit recording techniques. This technique allows for investigation of neuronal electrical activity in a manner that is not disruptive to the cell membrane, and individual neurons can be recorded from for extended periods of time. This chapter discusses the basic requirements for an extracellular recording setup, including different types of electrodes, apparatus for controlling electrode position and placement, recording equipment, signal output, data analysis, and the histological confirmation of recording sites usually required for in vivo recordings. A more advanced extracellular recording technique using piggy-back style multibarrel electrodes that allows for localized pharmacological manipulation of neuronal properties is described in detail. Strategies for successful signal isolation, troubleshooting advice such as noise reduction, and suggestions for general laboratory equipment are also discussed.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "In vivo electrophysiology recording"

1

Simonnet, Jean, Louis Richevaux, and Desdemona Fricker. "Single or Double Patch-Clamp Recordings In Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics." In Patch Clamp Electrophysiology, 285–309. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_15.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Hill, Charlotte L., and Gary J. Stephens. "An Introduction to Patch Clamp Recording." In Patch Clamp Electrophysiology, 1–19. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Zhou, Yi, He Li, and Zhongju Xiao. "In Vivo Patch-Clamp Studies." In Patch Clamp Electrophysiology, 259–71. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Coddington, Luke T., and Joshua T. Dudman. "In Vivo Optogenetics with Stimulus Calibration." In Patch Clamp Electrophysiology, 273–83. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_14.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Leng, Gareth, and Nancy Sabatier. "Electrophysiology of Magnocellular Neuronsin Vivo." In Neurophysiology of Neuroendocrine Neurons, 1–28. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118606803.ch1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

von Dincklage, F., and B. Rehberg. "In-vivo Electrophysiology of Anesthetic Action." In Sleep and Anesthesia, 243–55. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4614-0173-5_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Richardson, Eric S., and Yong-Fu Xiao. "Electrophysiology of Single Cardiomyocytes: Patch Clamp and Other Recording Methods." In Cardiac Electrophysiology Methods and Models, 329–48. Boston, MA: Springer US, 2010. http://dx.doi.org/10.1007/978-1-4419-6658-2_16.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Stanfa, Louise C., and Anthony H. Dickenson. "In Vivo Electrophysiology of Dorsal-Horn Neurons." In Pain Research, 139–53. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1007/978-1-59259-770-3_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Deng, Ping, and Zao C. Xu. "Intracellular Recording In Vivo and Patch-Clamp Recording on Brain Slices." In Springer Protocols Handbooks, 105–21. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-576-3_7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Furue, Hidemasa. "In Vivo Blind Patch-Clamp Recording Technique." In Springer Protocols Handbooks, 171–82. Tokyo: Springer Japan, 2012. http://dx.doi.org/10.1007/978-4-431-53993-3_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "In vivo electrophysiology recording"

1

Rivnay, Jonathan. "Organic transistors for electrophysiology (Presentation Recording)." In SPIE Organic Photonics + Electronics, edited by Iain McCulloch, Oana D. Jurchescu, Ioannis Kymissis, Ruth Shinar, and Luisa Torsi. SPIE, 2015. http://dx.doi.org/10.1117/12.2188315.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Scholvin, J., C. G. Fonstad, and E. S. Boyden. "Scaling models for microfabricated in vivo neural recording technologies." In 2017 8th International IEEE/EMBS Conference on Neural Engineering (NER). IEEE, 2017. http://dx.doi.org/10.1109/ner.2017.8008321.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Zarifi, Telnaz, Chung-Ching Peng, and Mohammad Hossein Zarifi. "Low-power amplifier for in-vivo EEG signal recording." In 2011 1st Middle East Conference on Biomedical Engineering (MECBME). IEEE, 2011. http://dx.doi.org/10.1109/mecbme.2011.5752055.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Keshtkaran, M. R., and Zhi Yang. "Power line interference cancellation in in-vivo neural recording." In 2012 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2012. http://dx.doi.org/10.1109/embc.2012.6347169.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Jankowska-Kuchta, Elzbieta B., Jaroslaw W. Jaronski, and Ewa Lukaszewicz. "In-vivo recording the birefringence of the human cornea." In Barcelona - DL tentative, edited by Hans-Jochen Foth, Renato Marchesini, Halina Podbielska, Michel Robert-Nicoud, and Herbert Schneckenburger. SPIE, 1996. http://dx.doi.org/10.1117/12.229992.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Schwerdt, Helen N., Minjung Kim, Ekin Karasan, Satoko Amemori, Daigo Homma, Hideki Shimazu, Tomoko Yoshida, Robert Langer, Ann M. Graybiel, and Michael J. Cima. "Subcellular electrode arrays for multisite recording of dopamine in vivo." In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2017. http://dx.doi.org/10.1109/memsys.2017.7863465.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Zbrzeski, Adeline, Paul Hasler, Florian Kolbl, Emilie Syed, Noelle Lewis, and Sylvie Renaud. "A programmable bioamplifier on FPAA for in vivo neural recording." In 2010 IEEE Biomedical Circuits and Systems Conference (BioCAS). IEEE, 2010. http://dx.doi.org/10.1109/biocas.2010.5709584.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Shrivastav, Maneesh, and Paul Iaizzo. "In vivo cardiac monophasic action potential recording using electromyogram needles." In 2006 IEEE Biomedical Circuits and Systems Conference - Healthcare Technology (BioCas). IEEE, 2006. http://dx.doi.org/10.1109/biocas.2006.4600350.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Lopez, C. M., M. Welkenhuysen, S. Musa, W. Eberle, C. Bartic, R. Puers, and G. Gielen. "Towards a noise prediction model for in vivo neural recording." In 2012 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2012. http://dx.doi.org/10.1109/embc.2012.6346042.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Cota, Oscar F., Mario Schlosser, Michael Schiek, Thomas Stieglitz, Mortimer Gierthmuehlen, and Dennis Plachta. "iNODE in-vivo testing for selective vagus nerve recording and stimulation." In 2015 7th International IEEE/EMBS Conference on Neural Engineering (NER). IEEE, 2015. http://dx.doi.org/10.1109/ner.2015.7146675.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'In vivo electrophysiology recording' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography