Journal articles: 'In vivo Electrophysiology'

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Adcock, J. "Airway nerves: in vivo electrophysiology." Current Opinion in Pharmacology 2, no. 3 (June 1, 2002): 280–82. http://dx.doi.org/10.1016/s1471-4892(02)00153-4.

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Volkov, Alexander G., Eunice K. Nyasani, Clayton Tuckett, Esther A. Greeman, and Vladislav S. Markin. "Electrophysiology of pumpkin seeds: Memristors in vivo." Plant Signaling & Behavior 11, no. 4 (February 29, 2016): e1151600. http://dx.doi.org/10.1080/15592324.2016.1151600.

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Erofeev, Alexander, Ivan Antifeev, Anastasia Bolshakova, Ilya Bezprozvanny, and Olga Vlasova. "In Vivo Penetrating Microelectrodes for Brain Electrophysiology." Sensors 22, no. 23 (November 23, 2022): 9085. http://dx.doi.org/10.3390/s22239085.

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In recent decades, microelectrodes have been widely used in neuroscience to understand the mechanisms behind brain functions, as well as the relationship between neural activity and behavior, perception and cognition. However, the recording of neuronal activity over a long period of time is limited for various reasons. In this review, we briefly consider the types of penetrating chronic microelectrodes, as well as the conductive and insulating materials for microelectrode manufacturing. Additionally, we consider the effects of penetrating microelectrode implantation on brain tissue. In conclusion, we review recent advances in the field of in vivo microelectrodes.

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Pavenstädt, Hermann, and Martin Bek. "Podocyte electrophysiology, in vivo and in vitro." Microscopy Research and Technique 57, no. 4 (May 7, 2002): 224–27. http://dx.doi.org/10.1002/jemt.10078.

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Ashbaugh, Ryan C., Lalita Udpa, Ron R. Israeli, Assaf A. Gilad, and Galit Pelled. "Bioelectromagnetic Platform for Cell, Tissue, and In Vivo Stimulation." Biosensors 11, no. 8 (July 25, 2021): 248. http://dx.doi.org/10.3390/bios11080248.

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Magnetogenetics is a new field that utilizes electromagnetic fields to remotely control cellular activity. In addition to the development of the biological genetic tools, this approach requires designing hardware with a specific set of demands for the electromagnets used to provide the desired stimulation for electrophysiology and imaging experiments. Here, we present a universal stimulus delivery system comprising four magnet designs compatible with electrophysiology, fluorescence and luminescence imaging, microscopy, and freely behaving animal experiments. The overall system includes a low-cost stimulation controller that enables rapid switching between active and sham stimulation trials as well as precise control of stimulation delivery thereby enabling repeatable and reproducible measurements.

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Berul, Charles I., Mark J. Aronovitz, Paul J. Wang, and Michael E. Mendelsohn. "In Vivo Cardiac Electrophysiology Studies in the Mouse." Circulation 94, no. 10 (November 15, 1996): 2641–48. http://dx.doi.org/10.1161/01.cir.94.10.2641.

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Liang, Yao-Wen, Ming-Liang Lai, Feng-Mao Chiu, Hsin-Yi Tseng, Yu-Chun Lo, Ssu-Ju Li, Ching-Wen Chang, Po-Chuan Chen, and You-Yin Chen. "Experimental Verification for Numerical Simulation of Thalamic Stimulation-Evoked Calcium-Sensitive Fluorescence and Electrophysiology with Self-Assembled Multifunctional Optrode." Biosensors 13, no. 2 (February 13, 2023): 265. http://dx.doi.org/10.3390/bios13020265.

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Owing to its capacity to eliminate a long-standing methodological limitation, fiber photometry can assist research gaining novel insight into neural systems. Fiber photometry can reveal artifact-free neural activity under deep brain stimulation (DBS). Although evoking neural potential with DBS is an effective method for mediating neural activity and neural function, the relationship between DBS-evoked neural Ca2+ change and DBS-evoked neural electrophysiology remains unknown. Therefore, in this study, a self-assembled optrode was demonstrated as a DBS stimulator and an optical biosensor capable of concurrently recording Ca2+ fluorescence and electrophysiological signals. Before the in vivo experiment, the volume of tissue activated (VTA) was estimated, and the simulated Ca2+ signals were presented using Monte Carlo (MC) simulation to approach the realistic in vivo environment. When VTA and the simulated Ca2+ signals were combined, the distribution of simulated Ca2+ fluorescence signals matched the VTA region. In addition, the in vivo experiment revealed a correlation between the local field potential (LFP) and the Ca2+ fluorescence signal in the evoked region, revealing the relationship between electrophysiology and the performance of neural Ca2+ concentration behavior. Concurrent with the VTA volume, simulated Ca2+ intensity, and the in vivo experiment, these data suggested that the behavior of neural electrophysiology was consistent with the phenomenon of Ca2+ influx to neurons.

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Annecchino, Luca A., and Simon R. Schultz. "Progress in automating patch clamp cellular physiology." Brain and Neuroscience Advances 2 (January 1, 2018): 239821281877656. http://dx.doi.org/10.1177/2398212818776561.

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Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since their inception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address both voltage- and ligand-gated channels, and showing the potential to play a pivotal role in drug discovery and biomedical research. Unfortunately, the cell suspension assays to which early systems were limited cannot recreate biologically relevant cellular environments, or capture higher order aspects of synaptic physiology and network dynamics. In vivo patch clamp electrophysiology has the potential to yield more biologically complex information and be especially useful in reverse engineering the molecular and cellular mechanisms of single-cell and network neuronal computation, while capturing important aspects of human disease mechanisms and possible therapeutic strategies. Unfortunately, it is a difficult procedure with a steep learning curve, which has restricted dissemination of the technique. Luckily, in vivo patch clamp electrophysiology seems particularly amenable to robotic automation. In this review, we document the development of automated patch clamp technology, from early systems based on multi-well plates through to automated planar-array platforms, and modern robotic platforms capable of performing two-photon targeted whole-cell electrophysiological recordings in vivo.

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TEPPER, JAMES M., and PHILIP M. GROVES. "In Vivo Electrophysiology of Central Nervous System Terminal Autoreceptors." Annals of the New York Academy of Sciences 604, no. 1 Presynaptic R (August 1990): 470–87. http://dx.doi.org/10.1111/j.1749-6632.1990.tb32013.x.

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Jayant, Krishna, Michael Wenzel, Yuki Bando, Jordan P. Hamm, Nicola Mandriota, Jake H. Rabinowitz, Ilan Jen-La Plante, et al. "Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology In Vivo." Cell Reports 26, no. 1 (January 2019): 266–78. http://dx.doi.org/10.1016/j.celrep.2018.12.019.

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Hunt, David L., Chongxi Lai, Richard D. Smith, Albert K. Lee, Timothy D. Harris, and Mladen Barbic. "Multimodal in vivo brain electrophysiology with integrated glass microelectrodes." Nature Biomedical Engineering 3, no. 9 (April 1, 2019): 741–53. http://dx.doi.org/10.1038/s41551-019-0373-8.

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Ravens, Ursula. "Sex differences in cardiac electrophysiology." Canadian Journal of Physiology and Pharmacology 96, no. 10 (October 2018): 985–90. http://dx.doi.org/10.1139/cjpp-2018-0179.

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Women have a longer QT interval than men, which appears to evolve after puberty suggesting that sex hormones have an influence on cardiac electrophysiology. Sex hormones do in fact regulate cardiac ion channels via genomic and nongenomic pathways. Women are at greater risk for life-threatening arrhythmias under conditions that prolong the QT interval. In addition, women exhibit greater sensitivity to QT interval–prolonging drugs. Female sex has also an impact on propensity to cardiovascular disease, including atrial fibrillation. However, ex vivo recorded atrial action potentials (APs) from female and male patients in atrial fibrillation did not exhibit significant differences in shape, except that APs from women had slower upstroke velocity. It is concluded that sex-related differences should be taken into account not only in the clinics, but also in basic research.

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Kaiser, Elisabeth, Qinghai Tian, Michael Wagner, Monika Barth, Wenying Xian, Laura Schröder, Sandra Ruppenthal, et al. "DREADD technology reveals major impact of Gq signalling on cardiac electrophysiology." Cardiovascular Research 115, no. 6 (October 13, 2018): 1052–66. http://dx.doi.org/10.1093/cvr/cvy251.

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Abstract Aims Signalling via Gq-coupled receptors is of profound importance in many cardiac diseases such as hypertrophy and arrhythmia. Nevertheless, owing to their widespread expression and the inability to selectively stimulate such receptors in vivo, their relevance for cardiac function is not well understood. We here use DREADD technology to understand the role of Gq-coupled signalling in vivo in cardiac function. Methods and results We generated a novel transgenic mouse line that expresses a Gq-coupled DREADD (Dq) in striated muscle under the control of the muscle creatine kinase promotor. In vivo injection of the DREADD agonist clozapine-N-oxide (CNO) resulted in a dose-dependent, rapid mortality of the animals. In vivo electrocardiogram data revealed severe cardiac arrhythmias including lack of P waves, atrioventricular block, and ventricular tachycardia. Following Dq activation, electrophysiological malfunction of the heart could be recapitulated in the isolated heart ex vivo. Individual ventricular and atrial myocytes displayed a positive inotropic response and arrhythmogenic events in the absence of altered action potentials. Ventricular tissue sections revealed a strong co-localization of Dq with the principal cardiac connexin CX43. Western blot analysis with phosphor-specific antibodies revealed strong phosphorylation of a PKC-dependent CX43 phosphorylation site following CNO application in vivo. Conclusion Activation of Gq-coupled signalling has a major impact on impulse generation, impulse propagation, and coordinated impulse delivery in the heart. Thus, Gq-coupled signalling does not only modulate the myocytes’ Ca2+ handling but also directly alters the heart’s electrophysiological properties such as intercellular communication. This study greatly advances our understanding of the plethora of modulatory influences of Gq signalling on the heart in vivo.

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Erofeev, Alexander, Ivan Antifeev, Egor Vinokurov, Ilya Bezprozvanny, and Olga Vlasova. "An Open-Source Wireless Electrophysiology System for In Vivo Neuronal Activity Recording in the Rodent Brain: 2.0." Sensors 23, no. 24 (December 10, 2023): 9735. http://dx.doi.org/10.3390/s23249735.

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Current trends in neurobiological research focus on analyzing complex interactions within brain structures. To conduct relevant experiments, it is often essential to employ animals with unhampered mobility and utilize electrophysiological equipment capable of wirelessly transmitting data. In prior research, we introduced an open-source wireless electrophysiology system to surmount these challenges. Nonetheless, this prototype exhibited several limitations, such as a hefty weight for the wireless module, redundant system components, a diminished sampling rate, and limited battery longevity. In this study, we unveil an enhanced version of the open-source wireless electrophysiology system, tailored for in vivo monitoring of neural activity in rodent brains. This new system has been successfully tested in real-time recordings of in vivo neural activity. Consequently, our development offers researchers a cost-effective and proficient tool for studying complex brain functions.

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Kodandaramaiah, Suhasa B., Giovanni Talei Franzesi, Brian Y. Chow, Edward S. Boyden, and Craig R. Forest. "Automated whole-cell patch-clamp electrophysiology of neurons in vivo." Nature Methods 9, no. 6 (May 6, 2012): 585–87. http://dx.doi.org/10.1038/nmeth.1993.

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Lee, Kenji, Nicole Carr, Alec Perliss, and Chandramouli Chandrasekaran. "WaveMAP for identifying putative cell types from in vivo electrophysiology." STAR Protocols 4, no. 2 (June 2023): 102320. http://dx.doi.org/10.1016/j.xpro.2023.102320.

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Beaulé, Christian, Daniel Granados-Fuentes, Luciano Marpegan, and Erik D. Herzog. "In vitro circadian rhythms: imaging and electrophysiology." Essays in Biochemistry 49 (June 30, 2011): 103–17. http://dx.doi.org/10.1042/bse0490103.

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In vitro assays have localized circadian pacemakers to individual cells, revealed genetic determinants of rhythm generation, identified molecular players in cell–cell synchronization and determined physiological events regulated by circadian clocks. Although they allow strict control of experimental conditions and reduce the number of variables compared with in vivo studies, they also lack many of the conditions in which cellular circadian oscillators normally function. The present review highlights methods to study circadian timing in cultured mammalian cells and how they have shaped the hypothesis that all cells are capable of circadian rhythmicity.

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Lee, Peter, Jorge G. Quintanilla, José M. Alfonso-Almazán, Carlos Galán-Arriola, Ping Yan, Javier Sánchez-González, Nicasio Pérez-Castellano, et al. "In vivo ratiometric optical mapping enables high-resolution cardiac electrophysiology in pig models." Cardiovascular Research 115, no. 11 (February 7, 2019): 1659–71. http://dx.doi.org/10.1093/cvr/cvz039.

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Abstract Aims Cardiac optical mapping is the gold standard for measuring complex electrophysiology in ex vivo heart preparations. However, new methods for optical mapping in vivo have been elusive. We aimed at developing and validating an experimental method for performing in vivo cardiac optical mapping in pig models. Methods and results First, we characterized ex vivo the excitation-ratiometric properties during pacing and ventricular fibrillation (VF) of two near-infrared voltage-sensitive dyes (di-4-ANBDQBS/di-4-ANEQ(F)PTEA) optimized for imaging blood-perfused tissue (n = 7). Then, optical-fibre recordings in Langendorff-perfused hearts demonstrated that ratiometry permits the recording of optical action potentials (APs) with minimal motion artefacts during contraction (n = 7). Ratiometric optical mapping ex vivo also showed that optical AP duration (APD) and conduction velocity (CV) measurements can be accurately obtained to test drug effects. Secondly, we developed a percutaneous dye-loading protocol in vivo to perform high-resolution ratiometric optical mapping of VF dynamics (motion minimal) using a high-speed camera system positioned above the epicardial surface of the exposed heart (n = 11). During pacing (motion substantial) we recorded ratiometric optical signals and activation via a 2D fibre array in contact with the epicardial surface (n = 7). Optical APs in vivo under general anaesthesia showed significantly faster CV [120 (63–138) cm/s vs. 51 (41–64) cm/s; P = 0.032] and a statistical trend to longer APD90 [242 (217–254) ms vs. 192 (182–233) ms; P = 0.095] compared with ex vivo measurements in the contracting heart. The average rate of signal-to-noise ratio (SNR) decay of di-4-ANEQ(F)PTEA in vivo was 0.0671 ± 0.0090 min−1. However, reloading with di-4-ANEQ(F)PTEA fully recovered the initial SNR. Finally, toxicity studies (n = 12) showed that coronary dye injection did not generate systemic nor cardiac damage, although di-4-ANBDQBS injection induced transient hypotension, which was not observed with di-4-ANEQ(F)PTEA. Conclusions In vivo optical mapping using voltage ratiometry of near-infrared dyes enables high-resolution cardiac electrophysiology in translational pig models.

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Stoy, W. A., I. Kolb, G. L. Holst, Y. Liew, A. Pala, B. Yang, E. S. Boyden, G. B. Stanley, and C. R. Forest. "Robotic navigation to subcortical neural tissue for intracellular electrophysiology in vivo." Journal of Neurophysiology 118, no. 2 (August 1, 2017): 1141–50. http://dx.doi.org/10.1152/jn.00117.2017.

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This work represents an automated method for accessing subcortical neural tissue for intracellular electrophysiology in vivo. We have implemented a novel algorithm to detect obstructions during regional pipette localization and move around them while minimizing lateral displacement within brain tissue. This approach leverages computer control of pressure, manipulator position, and impedance measurements to create a closed-loop platform for pipette navigation in vivo. This technique enables whole cell patching studies to be performed throughout the living brain.

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Helbling, Saskia, Sundeep Teki, Martina F. Callaghan, William Sedley, Siawoosh Mohammadi, Timothy D. Griffiths, Nikolaus Weiskopf, and Gareth R. Barnes. "Structure predicts function: Combining non-invasive electrophysiology with in-vivo histology." NeuroImage 108 (March 2015): 377–85. http://dx.doi.org/10.1016/j.neuroimage.2014.12.030.

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LODGE, DAVID, ANN BOND, MICHAEL J. O'NEILL, CAROLINE A. HICKS, and MARTYN G. JONES. "Stereoselective Effects of 2,3-benzodiazepines In Vivo : Electrophysiology and Neuroprotection Studies." Neuropharmacology 35, no. 12 (December 1996): 1681–88. http://dx.doi.org/10.1016/s0028-3908(96)00155-4.

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Lujan, Heidi L., and Stephen E. DiCarlo. "Cardiac electrophysiology and the susceptibility to sustained ventricular tachycardia in intact, conscious mice." American Journal of Physiology-Heart and Circulatory Physiology 306, no. 8 (April 15, 2014): H1213—H1221. http://dx.doi.org/10.1152/ajpheart.00780.2013.

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Cardiac electrophysiological dysfunction is a major cause of death in humans. Accordingly, electrophysiological testing is routinely performed in intact, conscious, humans to evaluate arrhythmias and disorders of cardiac conduction. However, to date, in vivo electrophysiological studies in mice are limited to anesthetized open-chest or closed-chest preparations. However, cardiac electrophysiology in anesthetized mice or mice with surgical trauma may not adequately represent what occurs in conscious mice. Accordingly, an intact, conscious murine model of cardiac electrophysiology has the potential to be of major importance for advancing the concepts and methods that drive cardiovascular therapies. Therefore, we describe, for the first time, the use of an intact, conscious, murine model of cardiac electrophysiology. The conscious mouse model permits measurements of atrioventricular interval, sinus cycle length, sinus node recovery time (SNRT), SNRT corrected for spontaneous sinus cycle, Wenckebach cycle length, the ventricular effective refractory period (VERP) and the electrical stimulation threshold to induce sustained ventricular tachyarrhythmias in an intact, complex model free of the confounding influences of anesthetics and surgical trauma. This is an important consideration because anesthesia and surgical trauma markedly reduced cardiac output and heart rate as well as altered cardiac electrophysiology parameters. Most importantly, anesthesia and surgical trauma significantly increased the VERP and virtually eliminated the ability to induce sustained ventricular tachyarrhythmias. Accordingly, the methodology allows for the accurate documentation of cardiac electrophysiology in complex, conscious mice and may be adopted for advancing the concepts and ideas that drive cardiovascular research.

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Forro, Csaba, Davide Caron, Gian Angotzi, Vincenzo Gallo, Luca Berdondini, Francesca Santoro, Gemma Palazzolo, and Gabriella Panuccio. "Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology." Micromachines 12, no. 2 (January 24, 2021): 124. http://dx.doi.org/10.3390/mi12020124.

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Brain-on-Chip (BoC) biotechnology is emerging as a promising tool for biomedical and pharmaceutical research applied to the neurosciences. At the convergence between lab-on-chip and cell biology, BoC couples in vitro three-dimensional brain-like systems to an engineered microfluidics platform designed to provide an in vivo-like extrinsic microenvironment with the aim of replicating tissue- or organ-level physiological functions. BoC therefore offers the advantage of an in vitro reproduction of brain structures that is more faithful to the native correlate than what is obtained with conventional cell culture techniques. As brain function ultimately results in the generation of electrical signals, electrophysiology techniques are paramount for studying brain activity in health and disease. However, as BoC is still in its infancy, the availability of combined BoC–electrophysiology platforms is still limited. Here, we summarize the available biological substrates for BoC, starting with a historical perspective. We then describe the available tools enabling BoC electrophysiology studies, detailing their fabrication process and technical features, along with their advantages and limitations. We discuss the current and future applications of BoC electrophysiology, also expanding to complementary approaches. We conclude with an evaluation of the potential translational applications and prospective technology developments.

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Ellis, Lee D., Rüdiger Krahe, Charles W. Bourque, Robert J. Dunn, and Maurice J. Chacron. "Muscarinic Receptors Control Frequency Tuning Through the Downregulation of an A-Type Potassium Current." Journal of Neurophysiology 98, no. 3 (September 2007): 1526–37. http://dx.doi.org/10.1152/jn.00564.2007.

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The functional role of cholinergic input in the modulation of sensory responses was studied using a combination of in vivo and in vitro electrophysiology supplemented by mathematical modeling. The electrosensory system of weakly electric fish recognizes different environmental stimuli by their unique alteration of a self-generated electric field. Variations in the patterns of stimuli are primarily distinguished based on their frequency. Pyramidal neurons in the electrosensory lateral line lobe (ELL) are often tuned to respond to specific input frequencies. Alterations in the tuning of the pyramidal neurons may allow weakly electric fish to preferentially select for certain stimuli. Here we show that muscarinic receptor activation in vivo enhances the excitability, burst firing, and subsequently the response of pyramidal cells to naturalistic sensory input. Through a combination of in vitro electrophysiology and mathematical modeling, we reveal that this enhanced excitability and bursting likely results from the down-regulation of an A-type potassium current. Further, we provide an explanation of the mechanism by which these currents can mediate frequency tuning.

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Single, Sandra, and Alexander Borst. "Different Mechanisms of Calcium Entry Within Different Dendritic Compartments." Journal of Neurophysiology 87, no. 3 (March 1, 2002): 1616–24. http://dx.doi.org/10.1152/jn.00215.2001.

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From our experiments combining in vivo calcium imaging and electrophysiology on fly vertical motion-sensitive cells (VS-cells) during visual stimulation, we infer different mechanisms of calcium entry within different dendritic compartments; while in the main dendritic branches calcium influx from extracellular space takes place only via voltage-activated calcium channels (VACCs), calcium enters the dendritic tips through VACCs as well as nicotinic acetylcholine receptors (nAChRs). Consequently, neuronal nACHRs of insects have to be assumed to be permeable to some extent for calcium under in vivo conditions.

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FLETCHER, ROSS D., MARC WISH, and ANDREW COHEN. "The Use of the Implanted Pacemaker as an In Vivo Electrophysiology Laboratory." Journal of Electrophysiology 1, no. 5 (October 1987): 425–33. http://dx.doi.org/10.1111/j.1540-8167.1987.tb01433.x.

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Nicol, Alister U., Nicholas Perentos, Amadeu Q. Martins, and A. Jennifer Morton. "Automated detection and characterisation of rumination in sheep using in vivo electrophysiology." Physiology & Behavior 163 (September 2016): 258–66. http://dx.doi.org/10.1016/j.physbeh.2016.05.028.

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Gelzer, Anna R. M., Tim Attmann, Dirk Radicke, Daryl Nydam, Reto Candinas, and Georg Lutter. "Effects of Acute Systemic Endothelin Receptor Blockade on Cardiac Electrophysiology In Vivo." Journal of Cardiovascular Pharmacology 44, no. 5 (November 2004): 564–70. http://dx.doi.org/10.1097/00005344-200411000-00008.

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Kolb, Ilya, Gregory Holst, Brian Goldstein, Suhasa B. Kodandaramaiah, Edward S. Boyden, Eugenio Culurciello, and Craig R. Forest. "Automated, in-vivo, whole-cell electrophysiology using an integrated patch-clamp amplifier." BMC Neuroscience 14, Suppl 1 (2013): P131. http://dx.doi.org/10.1186/1471-2202-14-s1-p131.

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Morizio, James, and Vinson Go. "Proceedings #30: Implantable Neural Recording and Stimulation Technologies for in vivo Electrophysiology." Brain Stimulation 12, no. 2 (March 2019): e96-e97. http://dx.doi.org/10.1016/j.brs.2018.12.199.

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Gernert, Manuela, Angelika Richter, and Wolfgang Löscher. "In vivo extracellular electrophysiology of pallidal neurons in dystonic and nondystonic hamsters." Journal of Neuroscience Research 57, no. 6 (August 30, 1999): 894–905. http://dx.doi.org/10.1002/(sici)1097-4547(19990915)57:6<894::aid-jnr15>3.0.co;2-4.

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Yang, Yvonne, Ekin Reyhan, Marc Schubert, Robert Denninger, Niklas Wißmann, Rangel Pramatarov, Wolfgang Wick, Thomas Kuner, Frank Winkler, and Varun Venkataramani. "CNSC-30. IN VIVO DYNAMICS OF ASTROCYTE-GLIOBLASTOMA TUMOR NETWORKS." Neuro-Oncology 24, Supplement_7 (November 1, 2022): vii28—vii29. http://dx.doi.org/10.1093/neuonc/noac209.111.

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Abstract As one of the most aggressive incurable primary brain tumors, glioblastomas show a high invasivity and therapeutic resistance caused by their cellular and molecular heterogeneity. In previous studies, we showed that glioma cells interconnect using membrane protrusions called tumor microtubes to form a therapy-resistant malignant network. Here, we extend the concept of tumor networks to heterogeneous connectivity with the glial microenvironment. Using high-resolution light microscopy, ultrastructural tissue imaging, patch-clamp electrophysiology, intravital structural and functional microscopy, we characterize tumor-astrocyte connectivity. First, we used high-resolution light microscopy with immunohistochemistry and ultrastructural imaging to discover and characterize gap junctional connections between tumor cells and astrocytes. Next, we probed functional, heterotypic connections using dye filling with patch-clamp electrophysiology. Employing calcium imaging, we could demonstrate bidirectional communication patterns between tumor cells and astrocytes. We further characterized the stability of these astrocyte-glioma networks by using longitudinal imaging of single cells over several weeks in patient-derived xenograft models. For this, we used sulforhodamine 101 (SR101) as a marker for astrocyte-glioma connectivity to simultaneously visualize tumor cells and their glial microenvironment. The percentage of SR101 uptake in glioma cells increases over time, showing an increasing integration of tumor cells into the tumor-and astrocyte network during tumor evolution. Tumor cells, which are unconnected to other tumor cells or astrocytes, are the main drivers of invasion while a subgroup of glioma cells with stable astrocyte connectivity stay in place over several weeks. Lastly, we showed how these functional, heterotypic connections contribute to therapeutic resistance in the context of radiotherapy. In conclusion, we investigated multicellular networks between glioblastoma cells and astrocytes, their plasticity and role for therapeutic resistance.

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López-Jury, Luciana, Francisco García-Rosales, Eugenia González-Palomares, Johannes Wetekam, Michael Pasek, and Julio C. Hechavarria. "A neuron model with unbalanced synaptic weights explains the asymmetric effects of anaesthesia on the auditory cortex." PLOS Biology 21, no. 2 (February 21, 2023): e3002013. http://dx.doi.org/10.1371/journal.pbio.3002013.

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Substantial progress in the field of neuroscience has been made from anaesthetized preparations. Ketamine is one of the most used drugs in electrophysiology studies, but how ketamine affects neuronal responses is poorly understood. Here, we used in vivo electrophysiology and computational modelling to study how the auditory cortex of bats responds to vocalisations under anaesthesia and in wakefulness. In wakefulness, acoustic context increases neuronal discrimination of natural sounds. Neuron models predicted that ketamine affects the contextual discrimination of sounds regardless of the type of context heard by the animals (echolocation or communication sounds). However, empirical evidence showed that the predicted effect of ketamine occurs only if the acoustic context consists of low-pitched sounds (e.g., communication calls in bats). Using the empirical data, we updated the naïve models to show that differential effects of ketamine on cortical responses can be mediated by unbalanced changes in the firing rate of feedforward inputs to cortex, and changes in the depression of thalamo-cortical synaptic receptors. Combined, our findings obtained in vivo and in silico reveal the effects and mechanisms by which ketamine affects cortical responses to vocalisations.

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Abouassali, Obada, Mengmeng Chang, Bojjibabu Chidipi, Jose Luis Martinez, Michelle Reiser, Manasa Kanithi, Ravi Soni, et al. "In vitro and in vivo cardiac toxicity of flavored electronic nicotine delivery systems." American Journal of Physiology-Heart and Circulatory Physiology 320, no. 1 (January 1, 2021): H133—H143. http://dx.doi.org/10.1152/ajpheart.00283.2020.

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The use of electronic nicotine delivery systems (ENDS) is not harm free. It is not known whether ENDS negatively affect cardiac electrophysiological function. Our study in cell lines and in mice shows that ENDS can compromise cardiac electrophysiology, leading to action potential instability and inducible ventricular arrhythmias. Further investigations are necessary to assess the long-term cardiac safety profile of ENDS products in humans and to better understand how individual components of ENDS affect cardiac toxicity.

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Hong, Guosong, Tian-Ming Fu, Mu Qiao, Robert D. Viveros, Xiao Yang, Tao Zhou, Jung Min Lee, Hong-Gyu Park, Joshua R. Sanes, and Charles M. Lieber. "A method for single-neuron chronic recording from the retina in awake mice." Science 360, no. 6396 (June 28, 2018): 1447–51. http://dx.doi.org/10.1126/science.aas9160.

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The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

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Osborne, Jason E., and Joshua T. Dudman. "RIVETS: A Mechanical System for In Vivo and In Vitro Electrophysiology and Imaging." PLoS ONE 9, no. 2 (February 14, 2014): e89007. http://dx.doi.org/10.1371/journal.pone.0089007.

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Annecchino, Luca A., Alexander R. Morris, Caroline S. Copeland, Oshiorenoya E. Agabi, Paul Chadderton, and Simon R. Schultz. "Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology." Neuron 95, no. 5 (August 2017): 1048–55. http://dx.doi.org/10.1016/j.neuron.2017.08.018.

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Dawe, Gavin S., Keith D. Huff, Jim L. Vandergriff, Trevor Sharp, Michael J. O’Neill, and Kurt Rasmussen. "Olanzapine activates the rat locus coeruleus: in vivo electrophysiology and c-Fos immunoreactivity." Biological Psychiatry 50, no. 7 (October 2001): 510–20. http://dx.doi.org/10.1016/s0006-3223(01)01171-4.

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Geerts, Hugo, Kristophe Diaz, Athan Spiros, Andreas Jeromin, and Magali Haas. "P3-066: MECHANISTIC MODELING OF TAU PROPAGATION IN VIVO AND EFFECT ON ELECTROPHYSIOLOGY." Alzheimer's & Dementia 15 (July 2019): P951—P952. http://dx.doi.org/10.1016/j.jalz.2019.06.3093.

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Nunez, A., F. Amzica, and M. Steriade. "Electrophysiology of cat association cortical cells in vivo: intrinsic properties and synaptic responses." Journal of Neurophysiology 70, no. 1 (July 1, 1993): 418–30. http://dx.doi.org/10.1152/jn.1993.70.1.418.

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1. The intrinsic properties and synaptic responses of association cortical neurons (n = 179) recorded from cat's areas 5 and 7 were studied in vivo. Intracellular recordings were performed under urethane anesthesia. Resting membrane potential (Vm) was -71.7 +/- 1.2 (SE) mV, amplitude of action potential was 83.7 +/- 2.3 mV, and input resistance was 18.4 +/- 1.8 M omega. Cells were identified ortho- and antidromically from lateroposterior and centrolateral thalamic nuclei and from homotopic foci in the contralateral cortex. Physiologically identified neurons were intracellularly stained with Lucifer yellow (LY) and found to be pyramidal-shaped elements (n = 21). 2. We classified the neurons as regular-spiking and intrinsically bursting cells. Regular-spiking cells were further classified as slow- and fast-adapting according to the adaptation of spike frequency during long-lasting depolarizing current pulses. 3. Regular-spiking, slow-adapting neurons had a monophasic afterhyperpolarization (AHP) or a biphasic AHP with fast and medium components (FAHP, mAHP). Slow-adapting behavior was observed in 84% (n = 119) of the regular-spiking cells. 4. Regular-spiking, fast-adapting cells only fired a train of spikes at the beginning of the pulse. Thereafter, the Vm remained as a depolarizing plateau, occasionally triggering some spikes. These neurons had a monophasic AHP and represented 16% (n = 23) of the regular-spiking neurons. 5. Intrinsically bursting neurons (n = 37) were observed in 20% of neocortical cells at depolarized Vm. Their action potential was followed by a marked depolarizing afterpotential (DAP). Rhythmic (4-10 Hz) bursts occurred during long-lasting depolarizing current pulses. 6. Small (3-10 mV), fast (1.5-4 ms), all-or-none depolarizing potentials were triggered by depolarizing current pulses. They are tentatively regarded as dendritic spikes recorded from the soma because their rate of occurrence changed as a function of the Vm and they were eventually blocked by hyperpolarization. 7. Synaptic stimulation of either thalamic or homotopic contralateral cortical areas elicited a sequence of excitatory postsynaptic potentials (EPSPs) and inhibitory postsynaptic potentials (IPSPs). Two components of the EPSP were revealed. At a hyperpolarized Vm, the initial component of the EPSP increased in amplitude, whereas the secondary component was blocked. Repetitive (10 Hz) stimulation of the thalamus or contralateral cortex elicited incremental responses. The augmentation phenomenon was due to an increase in the secondary component of the EPSP. The cortically elicited augmenting responses survived extensive thalamic lesions. A short IPSP and a long-lasting IPSP were evoked by thalamic or cortical stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)

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Hadrava, V., P. Blier, T. Dennis, C. Ortemann, and C. De Montigny. "Characterization of 5-hydroxytryptamine1A properties of flesinoxan: In Vivo electrophysiology and hypothermia study." Neuropharmacology 34, no. 10 (October 1995): 1311–26. http://dx.doi.org/10.1016/0028-3908(95)00098-q.

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Li, Liming, Pengjia Cao, Mingjie Sun, Xinyu Chai, Kaijie Wu, Xun Xu, Xiaoxin Li, and Qiushi Ren. "Intraorbital optic nerve stimulation with penetrating electrodes: in vivo electrophysiology study in rabbits." Graefe's Archive for Clinical and Experimental Ophthalmology 247, no. 3 (November 7, 2008): 349–61. http://dx.doi.org/10.1007/s00417-008-0977-2.

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Brea Guerrero, Alfonso, Mikko Oijala, Shawn C. Moseley, Te Tang, Fred Fletcher, Yicheng Zheng, Lilliana M. Sanchez, Benjamin J. Clark, Bruce L. Mcnaughton, and Aaron A. Wilber. "An Integrated Platform forIn VivoElectrophysiology in Spatial Cognition Experiments." eneuro 10, no. 11 (November 2023): ENEURO.0274–23.2023. http://dx.doi.org/10.1523/eneuro.0274-23.2023.

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AbstractSpatial cognition research requires behavioral paradigms that can distinguish between different navigational elements, such as allocentric (map-like) navigation and egocentric (e.g., body centered) navigation. To fill this need, we developed a flexible experimental platform that can be quickly modified without the need for significant changes to software and hardware. In this paper, we present this inexpensive and flexible behavioral platform paired with software which we are making freely available. Our behavioral platform serves as the foundation for a range of experiments, and although developed for assessing spatial cognition, it also has applications in the nonspatial domain of behavioral testing. There are two components of the software platform, “Maze” and “Stim Trigger.” While intended as a general platform, presently both programs can work in conjunction with Neuralynx and Open Ephys electrophysiology acquisition systems, allowing for precise time stamping of neural events. The Maze program includes functionality for automatic reward delivery based on user defined zones. “Stim Trigger” permits control of brain stimulation via any equipment that can be paired with an Arduino board. We seek to share our software and leverage the potential by expanding functionality in the future to meet the needs of a larger community of researchers.

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Zhao, Yanan, Praloy Chakraborty, Julianna Tomassetti, Tasnia Subha, Stéphane Massé, Paaladinesh Thavendiranathan, Filio Billia, Patrick F. H. Lai, Husam Abdel-Qadir, and Kumaraswamy Nanthakumar. "Arrhythmogenic Ventricular Remodeling by Next-Generation Bruton’s Tyrosine Kinase Inhibitor Acalabrutinib." International Journal of Molecular Sciences 25, no. 11 (June 5, 2024): 6207. http://dx.doi.org/10.3390/ijms25116207.

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Cardiac arrhythmias remain a significant concern with Ibrutinib (IBR), a first-generation Bruton’s tyrosine kinase inhibitor (BTKi). Acalabrutinib (ABR), a next-generation BTKi, is associated with reduced atrial arrhythmia events. However, the role of ABR in ventricular arrhythmia (VA) has not been adequately evaluated. Our study aimed to investigate VA vulnerability and ventricular electrophysiology following chronic ABR therapy in male Sprague–Dawley rats utilizing epicardial optical mapping for ventricular voltage and Ca2+ dynamics and VA induction by electrical stimulation in ex-vivo perfused hearts. Ventricular tissues were snap-frozen for protein analysis for sarcoplasmic Ca2+ and metabolic regulatory proteins. The results show that both ABR and IBR treatments increased VA vulnerability, with ABR showing higher VA regularity index (RI). IBR, but not ABR, is associated with the abbreviation of action potential duration (APD) and APD alternans. Both IBR and ABR increased diastolic Ca2+ leak and Ca2+ alternans, reduced conduction velocity (CV), and increased CV dispersion. Decreased SERCA2a expression and AMPK phosphorylation were observed with both treatments. Our results suggest that ABR treatment also increases the risk of VA by inducing proarrhythmic changes in Ca2+ signaling and membrane electrophysiology, as seen with IBR. However, the different impacts of these two BTKi on ventricular electrophysiology may contribute to differences in VA vulnerability and distinct VA characteristics.

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Mawad, Damia, Catherine Mansfield, Antonio Lauto, Filippo Perbellini, Geoffrey W. Nelson, Joanne Tonkin, Sean O. Bello, et al. "A conducting polymer with enhanced electronic stability applied in cardiac models." Science Advances 2, no. 11 (November 2016): e1601007. http://dx.doi.org/10.1126/sciadv.1601007.

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Electrically active constructs can have a beneficial effect on electroresponsive tissues, such as the brain, heart, and nervous system. Conducting polymers (CPs) are being considered as components of these constructs because of their intrinsic electroactive and flexible nature. However, their clinical application has been largely hampered by their short operational time due to a decrease in their electronic properties. We show that, by immobilizing the dopant in the conductive scaffold, we can prevent its electric deterioration. We grew polyaniline (PANI) doped with phytic acid on the surface of a chitosan film. The strong chelation between phytic acid and chitosan led to a conductive patch with retained electroactivity, low surface resistivity (35.85 ± 9.40 kilohms per square), and oxidized form after 2 weeks of incubation in physiological medium. Ex vivo experiments revealed that the conductive nature of the patch has an immediate effect on the electrophysiology of the heart. Preliminary in vivo experiments showed that the conductive patch does not induce proarrhythmogenic activities in the heart. Our findings set the foundation for the design of electronically stable CP-based scaffolds. This provides a robust conductive system that could be used at the interface with electroresponsive tissue to better understand the interaction and effect of these materials on the electrophysiology of these tissues.

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Swift, Luther M., Morgan Burke, Devon Guerrelli, Marissa Reilly, Manelle Ramadan, Damon McCullough, Tomas Prudencio, et al. "Age-dependent changes in electrophysiology and calcium handling: implications for pediatric cardiac research." American Journal of Physiology-Heart and Circulatory Physiology 318, no. 2 (February 1, 2020): H354—H365. http://dx.doi.org/10.1152/ajpheart.00521.2019.

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Rodent models are frequently employed in cardiovascular research, yet our understanding of pediatric cardiac physiology has largely been deduced from more simplified two-dimensional cell studies. Previous studies have shown that postnatal development includes an alteration in the expression of genes and proteins involved in cell coupling, ion channels, and intracellular calcium handling. Accordingly, we hypothesized that postnatal cell maturation is likely to lead to dynamic alterations in whole heart electrophysiology and calcium handling. To test this hypothesis, we employed multiparametric imaging and electrophysiological techniques to quantify developmental changes from neonate to adult. In vivo electrocardiograms were collected to assess changes in heart rate, variability, and atrioventricular conduction (Sprague-Dawley rats). Intact, whole hearts were transferred to a Langendorff-perfusion system for multiparametric imaging (voltage, calcium). Optical mapping was performed in conjunction with an electrophysiology study to assess cardiac dynamics throughout development. Postnatal age was associated with an increase in the heart rate (181 ± 34 vs. 429 ± 13 beats/min), faster atrioventricular conduction (94 ± 13 vs. 46 ± 3 ms), shortened action potentials (APD80: 113 ± 18 vs. 60 ± 17 ms), and decreased ventricular refractoriness (VERP: 157 ± 45 vs. 57 ± 14 ms; neonatal vs. adults, means ± SD, P < 0.05). Calcium handling matured with development, resulting in shortened calcium transient durations (168 ± 18 vs. 117 ± 14 ms) and decreased propensity for calcium transient alternans (160 ± 18- vs. 99 ± 11-ms cycle length threshold; neonatal vs. adults, mean ± SD, P < 0.05). Results of this study can serve as a comprehensive baseline for future studies focused on pediatric disease modeling and/or preclinical testing. NEW & NOTEWORTHY This is the first study to assess cardiac electrophysiology and calcium handling throughout postnatal development, using both in vivo and whole heart models.

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Cully, D. F., H. Wilkinson, D. K. Vassilatis, A. Etter, and J. P. Arena. "Molecular biology and electrophysiology of glutamategated chloride channels of invertebrates." Parasitology 113, S1 (January 1996): S191—S200. http://dx.doi.org/10.1017/s0031182000077970.

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SUMMARYIn this chapter we summarize the available data on a novel class of ligand-gated anion channels that are gated by the neurotransmitter glutamate. Glutamate is classically thought to be a stimulatory neurotransmitter, however, studies in invertebrates have proven that glutamate also functions as an inhibitory ligand. The bulk of studies conducted in vivo have been on insects and crustaceans, where glutamate was first postulated to act on H-receptors resulting in a hyperpolarizing response to glutamate. Recently, glutamate-gated chloride channels have been cloned from several nematodes and Drosophila. The pharmacology and electrophysiological properties of these channels have been studied by expression in Xenopus oocytes. Studies on the cloned channels demonstrate that the invertebrate glutamate-gated chloride channels are the H-receptors and represent important targets for the antiparasitic avermectins.

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Keliris, Georgios A., Qinglin Li, Amalia Papanikolaou, Nikos K. Logothetis, and Stelios M. Smirnakis. "Estimating average single-neuron visual receptive field sizes by fMRI." Proceedings of the National Academy of Sciences 116, no. 13 (March 13, 2019): 6425–34. http://dx.doi.org/10.1073/pnas.1809612116.

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The noninvasive estimation of neuronal receptive field (RF) properties in vivo allows a detailed understanding of brain organization as well as its plasticity by longitudinal following of potential changes. Visual RFs measured invasively by electrophysiology in animal models have traditionally provided a great extent of our current knowledge about the visual brain and its disorders. Voxel-based estimates of population RF (pRF) by functional magnetic resonance imaging (fMRI) in humans revolutionized the field and have been used extensively in numerous studies. However, current methods cannot estimate single-neuron RF sizes as they reflect large populations of neurons with individual RF scatter. Here, we introduce an approach to estimate RF size using spatial frequency selectivity to checkerboard patterns. This method allowed us to obtain noninvasive, average single-neuron RF estimates over a large portion of human early visual cortex. These estimates were significantly smaller compared with prior pRF methods. Furthermore, fMRI and electrophysiology experiments in nonhuman primates demonstrated an exceptionally good match, validating the approach.

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Jia, Xiaoxuan, Joshua H. Siegle, Corbett Bennett, Samuel D. Gale, Daniel J. Denman, Christof Koch, and Shawn R. Olsen. "High-density extracellular probes reveal dendritic backpropagation and facilitate neuron classification." Journal of Neurophysiology 121, no. 5 (May 1, 2019): 1831–47. http://dx.doi.org/10.1152/jn.00680.2018.

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Different neuron types serve distinct roles in neural processing. Extracellular electrical recordings are extensively used to study brain function but are typically blind to cell identity. Morphoelectrical properties of neurons measured on spatially dense electrode arrays have the potential to distinguish neuron types. We used high-density silicon probes to record from cortical and subcortical regions of the mouse brain. Extracellular waveforms of each neuron were detected across many channels and showed distinct spatiotemporal profiles among brain regions. Classification of neurons by brain region was improved with multichannel compared with single-channel waveforms. In visual cortex, unsupervised clustering identified the canonical regular-spiking (RS) and fast-spiking (FS) classes but also indicated a subclass of RS units with unidirectional backpropagating action potentials (BAPs). Moreover, BAPs were observed in many hippocampal RS cells. Overall, waveform analysis of spikes from high-density probes aids neuron identification and can reveal dendritic backpropagation. NEW & NOTEWORTHY It is challenging to identify neuron types with extracellular electrophysiology in vivo. We show that spatiotemporal action potentials measured on high-density electrode arrays can capture cell type-specific morphoelectrical properties, allowing classification of neurons across brain structures and within the cortex. Moreover, backpropagating action potentials are reliably detected in vivo from subpopulations of cortical and hippocampal neurons. Together, these results enhance the utility of dense extracellular electrophysiology for cell-type interrogation of brain network function.

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Meng, Xin, Lex Huismans, Teun Huijben, Greta Szabo, Ruud van Tol, Izak de Heer, Srividya Ganapathy, and Daan Brinks. "A compact microscope for voltage imaging." Journal of Optics 24, no. 5 (April 1, 2022): 054004. http://dx.doi.org/10.1088/2040-8986/ac5dd5.

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Abstract Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.

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Journal articles on the topic 'In vivo Electrophysiology'

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