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Books on the topic 'In-vivo Experiments'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

R, Welch D., and Burger Max M, eds. Cancer metastasis: In vitro and in vivo experimental approaches. Elsevier, 2000.

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2

Charabi, Samih. Acoustic neuroma/vestibular Schwannoma in vivo and in vitro growth models: A clinical and experimental study. Scandinavian University Press, 1997.

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3

Ferster, David. Patch Clamp Recording in Vivo. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0002.

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Patch clamp recording in vivo allows an investigator to study intracellular membrane potentials in an intact organism (as opposed to cells in culture or acute brain slices). This technique is a reliable method of obtaining high-quality intracellular recordings from neurons, regardless of their size, in several parts of the mammalian brain. This chapter will describe the principles and practice of performing patch clamp experiments in vivo, beginning with a brief history of the technological developments that have made this technique possible.

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4

Campagnola, Luke, and Paul Manis. Patch Clamp Recording in Brain Slices. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0001.

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Patch clamp recording in brain slices allows unparalleled access to neuronal membrane signals in a system that approximates the in-vivo neural substrate while affording greater control of experimental conditions. In this chapter we discuss the theory, methodology, and practical considerations of such experiments including the initial setup, techniques for preparing and handling viable brain slices, and patching and recording signals. A number of practical and technical issues faced by electrophysiologists are also considered, including maintaining slice viability, visualizing and identifying h

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5

Arnold, Monica M., Lauren M. Burgeno, and Paul E. M. Phillips. Fast-Scan Cyclic Voltammetry in Behaving Animals. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199939800.003.0005.

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Gaining insight into the mechanisms by which neural transmission governs behavior remains a central goal of behavioral neuroscience. Multiple applications exist for monitoring neurotransmission during behavior, including fast-scan cyclic voltammetry (FSCV). This technique is an electrochemical detection method that can be used to monitor subsecond changes in concentrations of electroactive molecules such as neurotransmitters. In this technique, a triangular waveform voltage is applied to a carbon fiber electrode implanted into a selected brain region. During each waveform application, specific

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6

Odds, Frank C. Pathogenesis of fungal disease. Edited by Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum, and Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0008.

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The pathogenesis of fungal disease involves an interplay between fungal virulence factors and host immune responses. Most fungal pathogens are opportunists that preferentially invade hosts with immune defects, but the fact that relative pathogenicity varies between fungal species (and even between different strains within a species) is evidence that fungi have evolved multiple, different molecular virulence factors. Experiments in which genes encoding putative virulence attributes are specifically disrupted and the resulting mutants are tested for virulence in a range of vertebrate and inverte

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7

(Editor), Claude L. Malmendier, P. Alaupovic (Editor), and H. Bryan Brewer (Editor), eds. Hypercholesterolemia, Hypocholesterolemia, Hypertriglyceridemia, In Vivo Kinetics (Advances in Experimental Medicine and Biology). Springer, 1991.

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8

(Editor), Virginia J. Savin, and Thomas B. Wiegmann (Editor), eds. New in Vivo and in Vitro Imaging Techniques (International Review of Experimental Pathology). Academic Pr, 1996.

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9

(Editor), E. Heinen, M. P. Defresne (Editor), J. Boniver (Editor), and V. Geenen (Editor), eds. In vivo Immunology: Regulatory Process During Lymphopoiesis Immunopoiesis (Advances in Experimental Medicine and Biology). Springer, 1994.

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10

Dymarkowski, Steven. In Vivo Analysis & Characterization of Myocardial Ischemia & Infarction: Experimental Mri-Studies (Acta Biomedica Lovaniensia, 288). Leuven Univ Pr, 2003.

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11

Christiansen, Jan, Jorgen Kjems, and Jan Egebjerg. Cancer Metastasis: In Vitro and in Vivo Experimental Approaches (Laboratory Techniques in Biochemistry and Molecular Biology, V. 26). 5th ed. Elsevier Science Ltd, 1998.

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12

Wallentin, Niklas. Posterior Capsule Opacification: An Experimental Study in Vitro and in Vivo (Comprehensive Summaries of Uppsala Dissertations, 230). Uppsala Universitet, 2000.

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13

Samathanam, Glory K. An examination of spinal cord neuronal damage and in vivo neurotransmitter release in rats with acute experimental allergic encephalomyelitis. 1989.

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14

Weekers, Frank. Study of the Endocrine & Metabolic Dysfunction & Assessment of Hormonal Interventions in a Novel in Vivo Experimental Model of Critical Illness. Leuven Univ Pr, 2003.

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15

Odlind, Cecilia. The Role of Dopamine-Metabolising Enzymes in Renal Sodium Handling: An Experimental Study in Vivo (Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, 1026). Uppsala Universitet, 2001.

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16

Torstenson, Richard. Functional Regulation of the Dopaminergic System in Vivo: Experimental and Clinical Studies With Positron Emission Tomography (Comprehensive Summaries ... from the Faculty of Pharmacy, 203). Uppsala Universitet, 1999.

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17

Gilbert, Donald L. Design and analysis of motor-evoked potential data in pediatric neurobehavioral disorder investigations. Edited by Charles M. Epstein, Eric M. Wassermann, and Ulf Ziemann. Oxford University Press, 2012. http://dx.doi.org/10.1093/oxfordhb/9780198568926.013.0025.

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This article discusses how transcranial magnetic stimulation (TMS) can be used to study the pathophysiological substrata of pediatric neurological and neurobehavioural disorders and to provide practical guidance for future research. It outlines the substantial challenges inherent in studying in vivo the neurobiology of pediatric neurobehavioural disorders, such as safety, quantitative versus categorical measures, and challenges in correlational studies. It discusses ways in which TMS generates quantitative measures that may function as endophenotypes for neurobehavioural disorders. Combining T

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18

Wassermann, Eric M. Direct current brain polarization. Edited by Charles M. Epstein, Eric M. Wassermann, and Ulf Ziemann. Oxford University Press, 2012. http://dx.doi.org/10.1093/oxfordhb/9780198568926.013.0007.

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The transcranial application of weak direct current (DC) to the brain is an effective neuromodulation technique that has had more than a century of experimental and therapeutic use. Focal DC brain polarization is now undergoing renewed interest, because of the wide acceptance of TMS as a research tool and candidate treatment for brain disorders. The effects of static electrical fields on cortical neurons in vivo have been known since the advent of intracellular recording. These effects are highly selective for neurons oriented longitudinally in the plane of the electric field. DC can enhance c

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19

Wójcik-Gładysz, Anna. Ghrelin – hormone with many faces. Central regulation and therapy. The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 2020. http://dx.doi.org/10.22358/mono_awg_2020.

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Discovered in 1999, ghrelin, is one of the peptides co-creating the hypothalamicgastrointestinal axis, otherwise known as the brain-gut axis. Ghrelin participates in many physiological processes and spectrum of its activity is still being discovered. This 28 amino acid peptide ‒ a product of the ghrl gene, was found in all vertebrates and is synthesized and secreted mainly from enteroendocrine X/A cells located in the gastric mucosa of the stomach. Expression of the ghrelin receptor has been found in many nuclei of the hypothalamus involved in appetite regulation. Therefore it’s presumed that

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20

Kawamura, Masahito. Ketogenic Diet in a Hippocampal Slice. Edited by Detlev Boison. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780190497996.003.0021.

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The hippocampus is thought to be a good experimental model for investigating epileptogenesis in and/or antiepileptic therapy for temporal lobe epilepsy. The hippocampus is also a useful target for researching the ketogenic diet. This chapter focuses on electrophysiological recordings using hippocampal slices and introduces their use for studying the anticonvulsant effects underlying ketogenic diets. The major difficulty in using hippocampal slices is the inability to precisely reproduce the in vivo condition of ketogenic diet feeding in this in vitro preparation. Three different approaches are

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21

Frew, Anthony. Air pollution. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0341.

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Any public debate about air pollution starts with the premise that air pollution cannot be good for you, so we should have less of it. However, it is much more difficult to determine how much is dangerous, and even more difficult to decide how much we are willing to pay for improvements in measured air pollution. Recent UK estimates suggest that fine particulate pollution causes about 6500 deaths per year, although it is not clear how many years of life are lost as a result. Some deaths may just be brought forward by a few days or weeks, while others may be truly premature. Globally, household

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22

Skiba, Grzegorz. Fizjologiczne, żywieniowe i genetyczne uwarunkowania właściwości kości rosnących świń. The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 2020. http://dx.doi.org/10.22358/mono_gs_2020.

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Bones are multifunctional passive organs of movement that supports soft tissue and directly attached muscles. They also protect internal organs and are a reserve of calcium, phosphorus and magnesium. Each bone is covered with periosteum, and the adjacent bone surfaces are covered by articular cartilage. Histologically, the bone is an organ composed of many different tissues. The main component is bone tissue (cortical and spongy) composed of a set of bone cells and intercellular substance (mineral and organic), it also contains fat, hematopoietic (bone marrow) and cartilaginous tissue. Bones a

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