Academic literature on the topic 'Incubation environment'
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Journal articles on the topic "Incubation environment"
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Chandra, Pankaj, Sandeep Srivastav, and Bipin Shah. "Innovation, Incubation, and Incubator." Vikalpa: The Journal for Decision Makers 28, no. 2 (April 2003): 89–94. http://dx.doi.org/10.1177/0256090920030208.
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This panel discussion was a part of a programme ‘From Incubation to Enterprise’ which was conducted by Indian Institute of Management, Ahmedabad's (IIMA) Centre for Innovation, Incubation, and Entreprenuership (CIIE) on January 4, 2003. Professor Pankaj Chandra of IIMA led the discussion. The distinguished panelists were Mr Sandeep Srivastav and Professor Bipin Shah. Their experience and insight on the subject helped in understanding the process of incubation leading to enterprise. The panel discussion addressed the following questions: What is incubation? What is innovation? What is the process of incubation that is needed for commercializing an innovation? What role does an incubator play? The following important points were made by the panelists: An innovative entrepreneur should adapt to the demand of the market. The adaptation may be with regard to technology, marketing, business idea or business plan. The concept of incubation originated from the medical field. The concept of incubators is applied more to project ideas which have a high degree of uncertainty. The aim of the incubators is to facilitate the survival of such companies and also to nurture these companies for growth and success. Incubators play the role of risk-sharing and providing credibility to an idea as it progresses from being an idea to some kind of product. It minimizes uncertainty and increases the success rate of an enterprise that is at a very nascent stage. The difference among promotional efforts and hand-holding is that promotional effort is the assistance given for setting up a business whereas, in an incubator, it is more of hand-holding. The biggest benefit of being in an incubator is that it insulates you from the outside environment and hence an innovator can just concentrate on getting his idea fool-proof for the market. Incubators even provide the network, linking the innovators with societal resources. The network is of two kinds - knowledge network and social network. The role of venture capitalists in enterprise building is to provide linkages for a budding entrepreneur. But not many people are ready to give money at an idea level Professor Pankaj Chandra concluded the discussion by saying that the incubator is not just for supporting the innovator but also for ensuring that the idea is converted into an enterprise.
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MORRIS, SARA R. "AVIAN INCUBATION: BEHAVIOUR, ENVIRONMENT, AND EVOLUTION." Wilson Bulletin 114, no. 1 (March 2002): 148–49. http://dx.doi.org/10.1676/0043-5643(2002)114[0148:aibeae]2.0.co;2.
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SOCKMAN, KEITH W. "Avian Incubation: Behaviour, Environment, and Evolution." Condor 105, no. 1 (2003): 164. http://dx.doi.org/10.1650/0010-5422(2003)105[164:b]2.0.co;2.
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Brown, Chris. "Avian Incubation: Behaviour, Environment, and Evolution." African Zoology 37, no. 2 (October 2002): 263–64. http://dx.doi.org/10.1080/15627020.2002.11657187.
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Sockman, Keith W. "Avian Incubation: Behaviour, Environment, and Evolution." Condor 105, no. 1 (February 1, 2003): 164–66. http://dx.doi.org/10.1093/condor/105.1.164.
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Al-Dajani, Haya, Evangelos Dedoussis, Erika Watson, and Nikolaos Tzokas. "Graduate Entrepreneurship Incubation Environments." Industry and Higher Education 28, no. 3 (June 2014): 201–13. http://dx.doi.org/10.5367/ihe.2014.0205.
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The benchmarking framework developed in this study is specifically designed for higher education institutions to consider when developing environments to encourage entrepreneurship among their students, graduates and staff. The objective of the study was to identify key success factors of Graduate Entrepreneurship Incubator Environments (GEIEs) that nurture, encourage and promote entrepreneurship in higher education. Semi-structured interviews with stakeholders in five leading UK entrepreneurial universities were used to collect data on GEIEs. The framework is a non-prescriptive tool which can be used by universities as a practical mechanism for plotting and monitoring progress towards an enabling environment for entrepreneurial graduates.
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Soeb, Md Janibul Alam, Muhammad Rashed Al Mamun, Shamima Shammi, Minhaj Uddin, and Rukon Ahmed Eimon. "Design and Fabrication of Low-Cost Incubator to Evaluate Hatching Performance of Egg." European Journal of Engineering and Technology Research 6, no. 7 (December 3, 2021): 91–96. http://dx.doi.org/10.24018/ejeng.2021.6.7.2662.
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In this study, a poultry egg incubator was designed, fabricated, and tested to evaluate its hatching performance. The incubator consists of a microcontroller with egg turner trays and incubating chamber of 116 nos. of egg capacity. The hatchability of the developed incubator was 79.3% and 87.1% hatchability during manual and automatic trials respectively. The temperature in the developed incubator was within the acceptable incubation temperature ranges from 37.6 °C to 38.6 °C. The average relative humidity in the developed incubator was maintained at 63.6% at manual and 55-65% at automatic trial. The eggs were turned manually approximately at 6 hours of interval. On the other hand, in the automatically controlled trial, it was done by egg turner maintaining exactly 6 hours of interval. It is noted that the percentage of hatching in rice husk incubators is below 55% which is much below comparing with the developed incubator. Also, the newborn chickens in rice husk incubators are unhealthy as they don’t get a sufficient amount of heat. Besides, in the sand incubation technique, kerosene-based hurricane lamps are used which produce Carbon Dioxide. The developed incubator is environment friendly because it doesn’t produce any by-product that is responsible for harming the environment. Also, after the successful trials, we have found the benefit-cost ratio was 1.42 which was quite satisfactory. The egg incubator can maintain the optimum conditions for the hatching of the chicken eggs and is capable of incubating and hatching the chicken eggs effectively. If the developed incubator is commercially supplied to the end-user, it will be a beneficial process of hatching for the farmer of Bangladesh.
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Soeb, Md Janibul Alam, Muhammad Rashed Al Mamun, Shamima Shammi, Minhaj Uddin, and Rukon Ahmed Eimon. "Design and Fabrication of Low-Cost Incubator to Evaluate Hatching Performance of Egg." European Journal of Engineering and Technology Research 6, no. 7 (December 3, 2021): 91–96. http://dx.doi.org/10.24018/ej-eng.2021.6.7.2662.
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In this study, a poultry egg incubator was designed, fabricated, and tested to evaluate its hatching performance. The incubator consists of a microcontroller with egg turner trays and incubating chamber of 116 nos. of egg capacity. The hatchability of the developed incubator was 79.3% and 87.1% hatchability during manual and automatic trials respectively. The temperature in the developed incubator was within the acceptable incubation temperature ranges from 37.6 °C to 38.6 °C. The average relative humidity in the developed incubator was maintained at 63.6% at manual and 55-65% at automatic trial. The eggs were turned manually approximately at 6 hours of interval. On the other hand, in the automatically controlled trial, it was done by egg turner maintaining exactly 6 hours of interval. It is noted that the percentage of hatching in rice husk incubators is below 55% which is much below comparing with the developed incubator. Also, the newborn chickens in rice husk incubators are unhealthy as they don’t get a sufficient amount of heat. Besides, in the sand incubation technique, kerosene-based hurricane lamps are used which produce Carbon Dioxide. The developed incubator is environment friendly because it doesn’t produce any by-product that is responsible for harming the environment. Also, after the successful trials, we have found the benefit-cost ratio was 1.42 which was quite satisfactory. The egg incubator can maintain the optimum conditions for the hatching of the chicken eggs and is capable of incubating and hatching the chicken eggs effectively. If the developed incubator is commercially supplied to the end-user, it will be a beneficial process of hatching for the farmer of Bangladesh.
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Engler, Randall. "Heraeus cytomat® 6000 Series Incubators and Storage Systems from Kendro Laboratory Products." JALA: Journal of the Association for Laboratory Automation 5, no. 2 (April 2000): 38–41. http://dx.doi.org/10.1016/s1535-5535-04-00060-7.
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There are a number of cases in high throughput screening systems where a controlled environment is desired. These include incubation periods for cell-based assays, incubation for protein detection assays such as ELISA or fluorescence assays, and branching assays for mRNA detection. In addition, as the density of wells in microplates increases and well volumes become smaller, evaporation becomes a concern in all assays. The cytomat® 6000 is a robot accessible, automated CO2 incubator used for cell-based High Throughput Screening systems. The incubator provides superior environmental conditions, due to the unique access door at the back of the instrument, the PlateShuttle (see Figure 3 ). This small access opening insures that the environment inside the system (temperature, humidity and CO2) is undisturbed as microplates are accessed ( Figure 2 ). The system provides high speed, random (bar-coded) access to all microplate formats for 24, 96, 384 and 1536 well microplates (up to 261-microplate capacity). Other cytomat configurations offer refrigerated and low humidity environments.
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Paraol de Matos, Guilherme, Milena Meridimi Teixeira, and Clarissa Stefani Teixeira. "An analysis of the pre-incubation process." International Journal for Innovation Education and Research 8, no. 6 (June 1, 2020): 01–15. http://dx.doi.org/10.31686/ijier.vol8.iss6.2260.
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Innovation habitats have fostered entrepreneurship and assisted in the creation and development of innovative ventures. Among the existing innovation habitats, the pre-incubator has contributed to the creation of a successful business model. Thus, the current study aims to analyze the perception of the pre-incubated on the importance of pre-incubation in the development of their business. A case study was conducted through personal interviews with participants from the pre-incubator in Florianópolis. As a main result, it verified that the pre-incubator contributed to the development of the business model of its pre-incubated. In addition, it served as a learning environment on entrepreneurship and the business world. However, negative points also raised, such as management changes in the middle of the pre-incubation process. Finally, it noted that the environment positively evaluated by the informants, mainly as a place of knowledge sharing and development of their business models.
Dissertations / Theses on the topic "Incubation environment"
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Hope, Sydney Frances. "Consequences of avian parental incubation behavior for within-clutch variance in incubation temperature and offspring behavioral phenotypes." Diss., Virginia Tech, 2020. http://hdl.handle.net/10919/104144.
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Parents can have large effects on their offspring by influencing the early developmental environment. In birds, a major way that parents can influence the early developmental environment is through egg incubation. Not only is incubation necessary for hatching success, but small changes of <1C in average incubation temperature have large effects on post-hatch offspring morphology and physiology. However, incubation is energetically costly and time-consuming for parents, and thus parents must allocate resources between incubation and self-maintenance. This can lead to differences in parental incubation behavior and egg temperatures among and within populations. Understanding which factors influence incubation, and the subsequent effects for offspring, is crucial for understanding parental effects, non-genetic drivers of phenotypic variation, and how environmental changes affect avian populations. I used wood ducks (Aix sponsa) as a study species to investigate how factors (disturbance, clutch size, ambient temperature) that influence parental demands may affect parental incubation behavior, physiology, and egg temperatures, and subsequently how egg temperatures affect offspring behavior and physiology. In a field experiment, I found that nest disturbance (i.e., capture) reduced both parent prolactin concentrations and the amount of time that parents spent incubating (Chapter 1). Further, ambient temperature was positively and clutch size negatively related to egg temperatures. Notably, in large clutches, differences in average incubation temperature among eggs within nests were large enough (i.e., >1C) to lead to different offspring phenotypes within broods (Chapter 2). Then, in a series of experiments in which I controlled incubation temperature, I provided evidence that lower average incubation temperatures lead to a reduced ability of ducklings to exit the nest cavity (Chapter 3), a more proactive behavioral phenotype (Chapter 4), a smaller body size, and a reduced efficiency in food consumption (Chapter 5), compared to those incubated at higher temperatures. Together, my dissertation illustrates how disturbances, clutch size, and ambient temperature can influence an important aspect of avian parental care, which has wide-ranging effects on offspring traits and fitness. This has broad implications for understanding the evolution of clutch size, development of behavior, and the effects of anthropogenic changes on wildlife.Doctor of Philosophy
Animal parents can have large effects on the development of their offspring. In birds, an important way that parents affect their offspring is through incubation, where parents physically warm their eggs to stimulate embryo development. Eggs must be incubated in order to hatch, but recent research has shown that small changes (<1C/2F) in average incubation temperature have major consequences for the quality (e.g., size, ability to thermoregulate) of offspring after they hatch. However, parents must balance how they spend their time and energy between incubation and other important activities (e.g., eating, avoiding predators), and thus incubation behavior and temperature can vary among birds. Understanding which factors affect incubation, and the consequences of altered incubation temperatures for the offspring, will help us to better understand how animals care for their offspring and how environmental changes may influence offspring development. I investigated how human disturbance, environmental temperature, and the number of eggs in the nest influenced parental incubation behavior and egg temperatures, and subsequently how egg temperatures affected offspring behaviors. By studying wood ducks (Aix sponsa) in the wild, I found that parents spent less time incubating after a human disturbance (i.e., capture) than before (Chapter 1), egg temperatures increased as environmental temperatures increased, and egg temperatures decreased as the number of eggs in the nest increased (Chapter 2). Further, in nests with many eggs (>12), some eggs experienced much lower average incubation temperatures (>1C/2F difference) than others in the same nest (Chapter 2). Then, by studying wood duck ducklings in an aviary, I found that ducklings incubated at lower temperatures were less successful at exiting a nest (Chapter 3), exhibited bolder and more exploratory behaviors (Chapter 4), were smaller, and consumed less food (Chapter 5), than those incubated at a higher temperature. Together, my dissertation shows that the number of eggs in a nest, environmental temperatures, and human disturbances can influence parental behaviors, which then affect offspring. This has broad implications for understanding why birds lay the number of eggs that they do, how animal behaviors develop, and how environmental changes (including those caused by humans) can affect wildlife.
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Dames, Ricardo Shane. "Best practices to create an enabling environment for SME incubation in South Africa." Thesis, Nelson Mandela Metropolitan University, 2012. http://hdl.handle.net/10948/d1015031.
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The humble beginnings of business incubators date back to the 1970s in the USA and United Kingdom, where abandoned industrial buildings were converted to rent out to small businesses. South Africa (SA) was first introduced to business incubation in the 1980s when the Small Business Development Corporation (SBDC) established a number of business ‘hives’ and provided business space to entrepreneurs to operate their businesses. In their most basic form, business incubators provide a safe and nurturing environment for entrepreneurs to establish their small businesses. While in incubation, the Small and Medium Enterprises (SMEs) are supported with a number of services which assist their growth and development until they are able to exit the incubator as sustainable and viable businesses. Global incubator models have matured significantly, and now include assisting with business idea generation, accelerating business start-ups and commercialisation processes, and identifying high-growth orientated SMEs to assist them with gaining market access. The establishment of business incubators was a government-driven initiative, and more than 33 SME incubators exist in SA. Most of the incubators are government-funded, and have focused on the establishment and growth of SMEs to act as a catalyst to promote economic development and alleviate socio-economic challenges such as unemployment and poverty. Despite these noble objectives, incubation in SA has not been fully utilised, and a high SME failure rate still prevails in the country. One of the reasons for SME failure may be ascribed to the lack of an enabling SME incubation environment in SA. When compared to other developing countries such as Brazil with as many as 400 incubators, it is clear that the SA incubation industry still needs further development. The purpose of this study therefore was to ascertain the best practices of global business incubators in both developed and developing countries, and how SA incubators could learn from these best practices to create a more conducive and enabling SME incubation environment. Background literature on business incubation with a specific focus on best practices in world incubators in both developed and developing countries, was reviewed. Some of the literature reviewed included Aernoudt (2004), Buys and Mbewana (2007) and Chandra (2009). From the literature review, four main best practice areas were identified, namely strategic focus, sources of funding, incubator services, and the role of government. The study followed a qualitative approach, and an interview schedule was developed to seek the perceptions of incubator managers on how the four identified best practices can be utilised to create an enabling SA incubation environment. A survey was conducted by interviewing 14 incubator managers (twelve government and two private) in SA. Data was collected over a six-month period, using face-to-face and telephonic interviews. Data was analysed using the content analysis, constant comparison, grounded theory and case study methods. The biographical profiles of the incubator managers and incubators were presented in case studies. An initial analysis was made to identify themes and sub themes within the four best practices explored, using the constant comparison method. Thereafter a provincial comparative analysis was made, as well as a comparison of government funded versus privately funded incubators. The findings suggested that there is a relatively high turnover in incubator management, and that they often do not have incubator management experience. A provincial comparison of SA incubators revealed that their strategic focuses are greatly influenced by the industry prevailing in the various provinces. All SA incubators provide pre- and post incubation services, but few are utilising virtual incubation. Most SA incubators are government-funded, and some use a hybrid funding model. It was evident that most SA incubators are aware of government policies and programmes available to assist them on local, provincial and national levels. A comparison of the best practices of two developing countries and three developed countries, as well as suggestions made by SA incubator managers indicated that SA has indeed followed best practices from both developing and developed countries, but there are areas of non-alignment which provide scope for improvement, to create a more conducive and enabling incubation environment. This study recommends that incubators should have an entrepreneurial focus, and reposition and align their strategic focus with government policies and instruments. SA incubators must pursue opportunities for virtual incubation as well as linkages with academic institutions, in order to offer value-added services such as research, development and commercialisation of the product ideas. Incubators in SA must pursue a hybrid funding model with a combination of government and private funding, and generate some or their own income. With regard to the role of government, it is recommended that the roll-out of more industry-specific incubators be privatised, and that an overseeing body for incubation be established.
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MacDonald, Elizabeth Catherine. "Songbird incubation dilemmas in the alpine: managing parent-offspring trade-offs in a variable thermal environment." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/42438.
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Small-bodied bird species exhibiting single-sex incubation must expend energy to create a buffered thermal environment for their eggs, while also meeting their own energetic requirements. The resultant trade-off between incubation and foraging is intensified in cold environments like the alpine, where energetic constraints are high. Ambient temperature influences incubation behaviour, with variable relationships across species, habitats and populations. I examined incubation rhythms of an alpine population of Horned Larks (Eremophila alpestris) in British Columbia across four years with different thermal regimes (2005 = moderate, 2006 = warm, 2010 = cold overnight, 2011 = cold during day) to determine whether incubating larks exhibited variable relationships between attentivity and temperature under different thermal conditions and how this related to management of the parent-offspring trade-off.
Early in the morning, females had to leave their nest to forage to reduce their energy deficit following 7 h of night incubation in near freezing conditions. Since temperatures at this time were still < 5˚C, embryos were at a high risk of potentially lethal chilling when females left the nest. From 06:00 to 08:00, incubating larks reduced attentiveness to 75% from 94% overnight. However, during this time period, females in 2010 spent more time off the nest as temperatures warmed than in other years. Throughout the study, adults took occasional “extended recesses” (incubation breaks lasting ≥ 59 min). These recesses were longer and more frequent in cold years, particularly 2011, and appeared to be largely related to harsh weather events. There are potential fitness consequences associated with these extended recesses, as egg hatching rate dropped to 81% in 2011 from 94% in the other years, and the incubation period was lengthened in two nests that engaged in multiple extended recesses.
Overall, this work demonstrated that incubating larks favour themselves when coping with difficult thermal conditions. Lark embryos appeared more tolerant to long periods of considerable cooling than previously thought, although, the reduced hatching rate in one cold year suggested there may be a limit to their cold tolerance.
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Porter, Pamela E. "Comparison between the subsurface environment of brown trout (Salmo trutta) redd and nonredd sites in two North Carolina streams." Thesis, Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/101252.
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The gravel environment of 30 brown trout (Salmo trutta) redds and adjacent nonredd sites in two western North Carolina streams were studied during the incubation period in 1979-1980 and 1980-1981. Intragravel water temperature, dissolved oxygen concentration, and percent oxygen saturation were highly correlated with surface water measurements, indicating that intragravel water is of surface origin. Permeability ranged from 250 to 149,350 cm/hr and averaged 6,150 cm/hr. Apparent velocity varied from 0 to 1,000 cm/hr and averaged 30 cm/hr. Permeability in redds was significantly greater than at nonredd sites. No significant differences in apparent velocity were found between redd and nonredd sites. No consistent differences in permeability or apparent velocity were found between streams or over time. Permeability and apparent velocity decreased significantly with depth.
Freeze cores were collected from redd and nonredd sites and divided into three 10-cm layers for analysis. Geometric mean diameter, sorting coefficient, fredle index, percent fines <2.00 mm, and percent porosity were highly variable and averaged 11.8 mm, 2.8, 4.2, 17.0 percent, and 19.0 percent, respectively. No significant differences were found among factors tested. Correlations between these gravel indices and permeability and apparent velocity were low. The gravel and intragravel environments appeared to be adequate for larval survival. Measurements did not reveal any clear trends during the incubation period. Brown trout did not by choice or redd construction appear to select or create (by redd construction) a subsurface environment different from the surrounding stream bed.M.S.
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Ling, Jonathan, and Gustav Lindstrand. "Optimizable Hydroponic Plant Incubator : Building a hydroponic plant incubator with a highly optimizable environment." Thesis, KTH, Mekatronik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-295805.
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This report investigates how to build a compact, optimizable and at the same time user friendly hydroponic systemfor growing plants as efficiently as possible. Hydroponics grows plants using water with dissolved nutrients instead of soil, allowing faster and more efficient growth.The focus has been on the implementation and the usability of such a system, centering around monitoring and to some degree controlling important parameters for growth such as humidity, air temperature, nutrition concentration and light intensity, colour and exposure time. The plant is enclosed in a confined space with artificial lighting which allows thorough control of the light environment. In order to achieve the desired level of control over the growth parameters, several sensors along with a microcontroller were used. A touch screen with a custom built graphical user interface was also connected to allow the user to control and monitor important aspects of growth conditions. The conclusion drawn from this project is that there is ahigh order of optimizability within the boundaries of this project. The measured factors are easily read on an intuitive, easily navigated touch screen for direct feedback. Regarding the lights’ effect on plant growth, the conclusionis drawn that plants grow well with many types of lights, but more time is needed to thoroughly investigate different light exposure times, colour and intensity.I denna rapport undersöks hur man kan bygga ett kompakt, optimerat och samtidigt användarvänligt hydroponiskt system för att odla växter så effektivt som möjligt. Hydroponiska system får plantor att växa genom att användavatten med näring istället för jord, vilket tillåter snabbare och mer effektiv tillväxt. Fokuset i denna rapport har varit på implementeringen och användbarheten av ett sådant system, med tyngpunkt på övervakning och till viss grad styrning av viktiga faktorer i en plantas tillväxt såsom luftfuktighet, temperatur, näringskoncentration och ljusintensitet, färg och exponeringstid. Växten är innesluten i ett begränsat utrymme med artificiellt ljus, vilket tillåter genomgående kontroll av ljusmiljön. För att uppnå önskad kontroll av tillväxtparametrarna, användes ett flertal sensorer tillsammans med en mikrokontroller. Till detta kopplades en pekskärm med ett egen tillverkat användargränssnitt, som tillåter användaren att kontrollera och övervaka viktiga aspekter i tillväxten. Slutsatsen från detta projekt är att det finns en hög grad av optimerbarhet inom denna konstruktion. De uppmätta parametrarna kan enkelt avläsa ljusexponering, ljusintesitet och färg.
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Kalyva, Maria. "Fate of pharmaceuticals in aquatic environments in Northern Sweden." Thesis, Umeå universitet, Institutionen för ekologi, miljö och geovetenskap, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-130304.
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The occurrence of pharmaceuticals in aquatic environments originating from human consumption has received increased scientific attention during the last decades due to concerns regarding their combined environmental effects. This concerns stress the need of studies quantifying dissipation rates of pharmaceutical in aquatic ecosystems. The aims of this study were: i) to assess the degradation rates of trimethoprim (TPR), diphenhydramine (DPH), diclofenac (DCL), oxazepam (OXZ) and hydroxyzine (HDZ) in laboratory incubations, and ii) to compare laboratory assessment of dissipation rates with previously measured in situ half-lives of these drugs in a pond ecosystem. I hypothesized that the dissipation of these five drugs dissolved, in laboratory incubations, is affected by common environmental parameters such as temperature, UV-light, organic solutes and presences of sediments. In line with my hypothesis, all substances were affected by my treatments. Here, main parameters affecting the dissipation of the drugs were UV light and to a lesser temperature (i.e. through microbial degradation). All drugs were found to be affected by sediment sorption, especially HDZ where 95 % of the applied pharmaceutical was adsorbed. Laboratory estimate with highest environmental relevance (low TOC and 3 °C water temperature or low TOC, sediments and UV light) seemed to predict field estimates fairly well for all of the drugs beside OXZ and DCL. Given the strong adsorption for sediments seen in the laboratory incubations, it seems likely that the mismatch between laboratory inferred half-lives and the in situ half-lives for OXZ was likely caused by sediment exchange processes releasing drugs initially adsorbed to the sediments into water column over time.
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Parker, Scott Landsborough. "Thermal and reproductive biology of the lizards sceloporus occidentalis and sceloporus graciosus along an environmental and geographical gradient." CSUSB ScholarWorks, 2001. https://scholarworks.lib.csusb.edu/etd-project/2010.
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The thermal environment exerts a strong influence on many aspectsof squamate life history. Environmental temperatures constrain daily activity time, fecundity, growth rate, and also influences hatchling phenotype. Cold nest temperatures can slow the development of eggs deposited in the soil, cause developmental abnormalities or result in mortality of embryos.
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Bolin, Greta M. "Incubation humidity as an environmental stressor on the osmoregulatory developmental program of the chicken, Gallus gallus domesticus." Thesis, University of North Texas, 2009. https://digital.library.unt.edu/ark:/67531/metadc11055/.
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Fetal programming results from stressors during fetal development and may influence the occurrence of disease later in life. Maternal nutritional status and/or environment can affect renal development by inducing limited nephron endowment at birth, which results in diseases such as hypertension and coronary heart disease in mammals. Birds are likely to be effective models for this process because, like mammals, they have high pressure cardiovascular systems, mammalian-type nephrons and are homeothermic. This project uses the chicken embryo to explore physiological responses of disrupted hydration state thereby providing insights into renal fetal programming. Under normal conditions the chorioallantoic membrane (CAM) and developing avian kidney work in unison to ensure a proper balance of ions and water within the egg. White leghorn chicken eggs were incubated at 37.5oC±0.5oC and either <35%, 55-60% (normal) or >85% relative humidity. Amniotic fluid serves as the drinking source for the embryo late in development; its composition is important to salt and water homeostasis. High amniotic fluid osmolality increased the blood osmolality for embryos exposed to low humidity incubation thereby indirectly influencing the renal developmental program of the embryos from this group. Indeed estimated filtering capacity was doubled in the low humidity group (6.77 ± 0.43 mm3) compared to normal (4.80 ± 0.33 mm3) and high (3.97 ± 0.30 mm3) humidity groups. The increased filtering capacity seen for those embryos from low humidity may indicate the ability for more efficient recovery of water if similarly stressed as an adult bird. All embryo populations maintained similar oxygen consumption (0.075 ml/min - 0.37 ml/min), hematocrit (15 % - 32 %) and hemoglobin values (4 g/dl - 9 g/dl), thus displaying control over these aspects of the internal environment despite the obvious environmental insult of extreme incubation humidity. These results signify the embryo's immature kidney, along with lower gastrointestinal tract, functions much like the adult form maintaining homeostasis, although the mechanisms may differ. The overall benefits of this research included better understanding of the role the kidney during embryonic development and determining whether environmental factors, such as humidity, leave an imprint on morphological and physiological aspects of the urinary system of the embryo and water compartments of the egg.
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Best, Zoey Ellen. "Environmental Factors Affecting Loggerhead Sea Turtle (Caretta caretta) Nesting, Hatching, and Incubation Patterns in Broward County, Florida." NSUWorks, 2017. http://nsuworks.nova.edu/occ_stuetd/446.
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Reproductive success in loggerhead (Caretta caretta) sea turtles is strongly dependent on the effective placement and internal conditions of their nests. Embryos rely on optimal incubation conditions for proper development and growth, which determines how many hatchlings will emerge from the nest. The internal microclimate of each nest is delicately balanced and can be easily influenced by external environmental conditions. This study was designed to examine several environmental variables and determine their effects on sea turtle nesting numbers, hatching success, and incubation conditions in Broward County Florida. Over a span of 25 years (1991-2015), the Broward County Sea Turtle Conservation Program has collected data on each sea turtle nest laid in Broward County. This data was analyzed and plotted to visualize nesting and hatching trends, and regressions were fitted to make comparisons to historic air temperature, sea surface temperature, precipitation, and lunar illumination data. These regressions were tested for significance, and each environmental variable was found to have varying levels of impact on sea turtle nesting and hatching behavior. Of the environmental variables considered in this study, analyses suggest that sea turtles are most responsive to temperature, with sea surface temperature serving as the best proxy for predicting nesting behaviors. Air temperature over the incubation period was found to be the best indicator for hatch success percentage. Air temperature, sea surface temperature, and precipitation averages all significantly affected the length of the incubation period. The regression models created in this study could be used to examine the interactions between climatic variables, and to indicate what impacts can be expected by these various environmental factors. This information could be used to estimate the future effects of climate change on sea turtle reproduction, and to predict general reproductive success and future population trends.
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Bolin, Greta M. Burggren Warren W. "Incubation humidity as an environmental stressor on the osmoregulatory developmental program of the chicken, Gallus gallus domesticus." [Denton, Tex.] : University of North Texas, 2009. http://digital.library.unt.edu/permalink/meta-dc-11055.
Full textBooks on the topic "Incubation environment"
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Deeming, D. Charles. Reptilian Incubation: Environment and Behaviour. Nottingham University Press, 2004.
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Charles, Deeming D., ed. Reptilian incubation: Environment, evolution and behaviour. Nottingham: Nottingham University Press, 2004.
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Charles, Deeming D., ed. Avian incubation: Behaviour, environment, and evolution. Oxford: Oxford University Press, 2002.
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Deeming, D. C. Avian Incubation: Behaviour, Environment, and Evolution (Oxford Ornithology Series, 13). Oxford University Press, USA, 2002.
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Environmental Leadership Capacity Building In Higher Education Experience And Lessons From Asian Program For Incubation Of Environmental Leaders. Springer Verlag, Japan, 2013.
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Kam, Yeong-Choy. Environmental influences on the critical oxygen tension, water balance, metabolism, and growth of reptilian embryos. 1992.
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Hanaki, Keisuke, and Takashi Mino. Environmental Leadership Capacity Building in Higher Education: Experience and Lessons from Asian Program for Incubation of Environmental Leaders. Springer, 2013.
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Karmali, Mohamed A., and Jan M. Sargeant. Verocytotoxin-producing Escherichia coli (VTEC) infections. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0008.
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Verocytotoxin (VT)-producing Escherichia coli (VTEC), also known as Shiga toxin producing E. coli (STEC), are zoonotic agents, which cause a potentially fatal illness whose clinical spectrum includes diarrhoea, haemorrhagic colitis, and the haemolytic uraemic syndrome (HUS). VTEC are of serious public health concern because of their association with large outbreaks and with HUS, which is the leading cause of acute renal failure in children. Although over 200 different OH serotypes of VTEC have been associated with human illness, the vast majority of reported outbreaks and sporadic cases of VTEC-infection in humans have been associated with serotype O157:H7.VTs constitute a family of related protein subunit exotoxins, the major ones implicated in human disease being VT1, VT2, and VT2c. Following their translocation into the circulation, VTs bind to endothelial cells of the renal glomeruli, and of other organs and tissues via a specific receptor globotriosylceramide (Gb 3), are internalized by a process of receptor-mediated endocytosis, and cause subcellular damage that results in the characteristic microangiopathic disease observed in HUS.The incubation period of VTEC-associated illness is about 3–5 days. After ingestion VTEC (especially of serotype O157:H7) multiply in the bowel and colonize the mucosa of probably the large bowel with a characteristic attaching and effacing (AE) cytopathology. Colonization is followed by the translocation of VTs into the circulation and the subsequent manifestation of disease.The majority of patients with uncomplicated VTEC infection recover fully with general supportive measures. Historically, the case-fatality rate was high for HUS. However, improvement in the treatment of renal failure and the attendant biochemical disturbances has substantially improved the outlook, although long-term sequelae may develop.Ruminants, especially cattle, are the main reservoirs of VTEC. Infection is acquired through the ingestion of contaminated food, especially under-cooked hamburger, through direct contact with animals, via contaminated water or environments, or via personto-person transmission.The occurrence of large outbreaks of food-borne VTEC-associated illness has promoted close scrutiny of this zoonoses at all levels in the chain of transmission, including the farm, abattoir, food processing, packaging and distribution plants, the wholesaler, the retailer and the consumer. While eradication of VTEC O157 at the farm may not be an option, interventions to increase animal resistance or to decrease animal exposure are being developed and validated. Hazard Analysis and Critical Control Programmes are being implemented in the processing sector and appear to be associated with temporal decreases in VTEC serotype O157 illness in humans. Education programmes targeting food handling procedures and hygiene practices are being advocated at the retail and consumer level. Continued efforts at all stages from the farm to the consumer will be necessary to reduce the risk of VTEC-associated illness in humans.
Book chapters on the topic "Incubation environment"
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Rutkowska, M., A. Heimowska, K. Krasowska, and M. Jastrzębska. "Comparison of Modified Polyethylene Incubation Effects in Seawater and Composting Natural Environment." In Advanced Materials, Polymers, and Composites, 367–76. New York: Apple Academic Press, 2021. http://dx.doi.org/10.1201/9781003105015-30.
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Żarski, Daniel, Ákos Horváth, Gergely Bernáth, Sławomir Krejszeff, János Radóczi, Katarzyna Palińska-Żarska, Zoltán Bokor, Krzysztof Kupren, and Béla Urbányi. "Incubation and Hatching." In SpringerBriefs in Environmental Science, 81–89. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-49376-3_10.
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Naga Raju, Maddela, Narasimha Golla, and Rangaswamy Vengatampalli. "Soil Incubation Studies." In SpringerBriefs in Environmental Science, 17. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-42655-6_4.
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Agostinho, Carlos, Fenareti Lampathaki, Ricardo Jardim-Goncalves, and Oscar Lazaro. "Accelerating Web-Entrepreneurship in Local Incubation Environments." In Lecture Notes in Business Information Processing, 183–94. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-19243-7_19.
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Harvey, Pierre-Léonard. "The Organization and Governance of an Incubation and Learning Environment with the Help of Tool Boxes: Reference Model, Community Informatics Design Framework and Instantiation Methodology." In Translational Systems Sciences, 345–409. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-65373-0_11.
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Akiyama, Tomohiro, Keisuke Hanaki, and Takashi Mino. "Asian Program for Incubation of Environmental Leaders." In Environmental Leadership Capacity Building in Higher Education, 1–18. Tokyo: Springer Japan, 2013. http://dx.doi.org/10.1007/978-4-431-54340-4_1.
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Lepik, Katri-Liis, and Audronė Urmanavičienė. "The Role of Higher Education Institutions in Development of Social Entrepreneurship: The Case of Tallinn University Social Entrepreneurship Study Program, Estonia." In Innovation, Technology, and Knowledge Management, 129–51. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-84044-0_7.
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AbstractThe purpose of this chapter is to introduce a higher education social enterprise program and explore how it is shaping the field of social entrepreneurship. Social enterprise related university programs are an emerging trend. Entrepreneurial university theory and ecosystem framework are used to illustrate how the university social enterprise program, in turn, develops the field of social entrepreneurship. An example of an existing social enterprise program is discussed to highlight how it can be designed. Cases of social enterprises emerged as the result of the program are used to outline the different impacts that such support to social entrepreneurship might have. The research chapter reveals the multi-dimensional nature of the social enterprise program and its impact on students establishing their own social enterprises. It suggests that the incubation and other support activities should expand beyond the university program including a variety of network partners. The chapter provides empirical evidence of social enterprise development in a higher education institution and contributes to the global body of knowledge about fostering social enterprise development. As the provision of social entrepreneurship education is new in Estonia and the discussions on social enterprises are premature, the number of social entrepreneurship development partners is limited and hence the empirical data is currently scarce. The journey towards an entrepreneurial university is limited due to the lack of legal support and suitable infrastructure which would enhance project-based learning, support ‘spin-offs’ and patenting and rather engenders a more traditional academic learning environment.
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Bakir, Vian, and Andrew McStay. "The Nature and Circulation of False Information." In Optimising Emotions, Incubating Falsehoods, 71–102. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-13551-4_4.
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AbstractThis chapter focuses on the nature of disinformation (false information spread with intent to deceive) and misinformation (false information spread without specific deceptive intent), inquiring into processes that increase their circulation online. As befits any study of media systems, it addresses interconnections between technologies, media forms, wider media and political environments, people and impacts. It opens with a discussion on the role of deception in citizen-political communications. This highlights the long-standing debate on whether political leaders should lie to their citizens, addressing evidence of such activity in the areas of national security and election campaigns. It then discusses the nature and scale of two key forms of contemporary disinformation: fake news and deepfakes. Widening the focus beyond intentionally deceptive forms to false information in general, the chapter then examines the dynamics of spreading false information online, discussing why people engage with such processes.
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Bakir, Vian, and Andrew McStay. "Core Incubators of False Information Online." In Optimising Emotions, Incubating Falsehoods, 29–52. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-13551-4_2.
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AbstractFalse information is incubated across complex, interconnected communication and technological environments, imbricating individuals and society. This chapter introduces two key concepts. The first is the economics of emotion: namely, the optimisation of datafied emotional content for financial gain. The second concept is the politics of emotion: namely, the optimisation of datafied emotional content for political gain. Optimising emotions entails understanding people in terms of demography, interests and disposition; creation of content (by machines or by people) optimised to resonate with profiled individuals and groups; strategic ambition to elicit emotion to cause contagion; and recording of this datafied emotion expression, to feed into the next wave of info-contagion. The chapter presents the economics of emotion as the core incubator of false information online, as this stems from the business model of globally dominant digital platforms while also enabling the business model of digital influence mercenaries. However, the politics of emotion readily exploits the tools at its disposal.
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Bakir, Vian, and Andrew McStay. "Feeling-Into the Civic Body: Affect, Emotions and Moods." In Optimising Emotions, Incubating Falsehoods, 103–37. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-13551-4_5.
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AbstractThis chapter accounts for the energising role of affect, emotions and moods in circulating false information throughout the civic body. It starts by charting the trajectory of the role of feelings in understanding citizen-political communications. Their persuasive importance was recognised millennia ago and have been recognised anew in recent decades with the advent of neuroscience and the understanding that emotions are important for decisions and judgements. The chapter highlights three main mechanisms through which governments can try to manage public feeling and hence behaviour: discursive, decision-making based and datafied. It then considers the prevalent claim that we live in a post-truth condition (where appeals to emotion and personal belief are more influential in shaping public opinion than objective facts). While the relative importance of emotion and facts in everyday life is difficult to ascertain, the chapter demonstrates that the media from which people would normally derive their facts (namely, news media and social media) have become more emotionalised and affective, and suggests that we live in an informational environment that is sub-optimal for a healthy civic body. Finally, the chapter examines the challenges faced by governments in managing their population’s feelings during the COVID-19 pandemic where uncertainty, anxiety and false information proliferate.
Conference papers on the topic "Incubation environment"
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Blain Christen, J. M., and A. G. Andreou. "CMOS heater array for incubation environment cellular study." In 48th Midwest Symposium on Circuits and Systems, 2005. IEEE, 2005. http://dx.doi.org/10.1109/mwscas.2005.1594468.
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Davey, Todd, Thorsten Kliewe, Peter van der Sijde, and Matt McIntyre. "Continuous high technology bursiness incubation." In 16th Annual High Technology Small Firms Conference, HTSF 2008. University of Twente, 2008. http://dx.doi.org/10.3990/2.268488823.
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This paper addresses the question of how organisations can foster high technology businesses in an incubation-type environment. We compare the approaches taken by a large private firm with a successful University entrepreneurial program to outline successful elements in creating a successful high tech business incubation environment. By outlining similarities and differences of the two cases, this paper provides practical insights how to foster high technology businesses.
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Mancer, Halima, and Mustapha Daddi Bouhoun. "Effect of irrigation water salinity on the organic carbon mineralization in soil (laboratory incubation)." In TECHNOLOGIES AND MATERIALS FOR RENEWABLE ENERGY, ENVIRONMENT AND SUSTAINABILITY: TMREES18. Author(s), 2018. http://dx.doi.org/10.1063/1.5039166.
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Anggraini, Novita, and Herdis Herdiansyah. "BOD determination of faecal sludge waste by modifying time and temperature in the incubation period." In THE 2ND INTERNATIONAL CONFERENCE ON SCIENCE, MATHEMATICS, ENVIRONMENT, AND EDUCATION. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5139738.
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Lam, Tony C. T., and Robert Dewey. "Probabilistic Analysis of Turbine Disc SCC Incubation and Propagation." In 2002 International Joint Power Generation Conference. ASMEDC, 2002. http://dx.doi.org/10.1115/ijpgc2002-26156.
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Stress corrosion cracking (SCC) is a common problem found on aging low-pressure turbine (LP) rotors that operate in a wet/dry stream environment. While much has been published on the growth rate of SCC in turbine rotor-disk materials, incubation time is rarely addressed. Since no effective way has been demonstrated to prevent disk rim SCC from occurring other than to replace the damaged rim with a weld repair of higher chromium content, a better understanding of incubation time could provide operators with a means to treat SCC before cracks are large enough to start to grow. This paper discusses the critical mechanisms involved in the SCC incubation, process and describes a probabilistic approach to make meaningful assessments of incubation time. Data published for General Electric turbine rotors is used to test the model.
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Fujikawa, Ryosuke, Shigeki Abe, Takao Nakamura, and Masayuki Kamaya. "Influence of PWR Environment on Fatigue Crack Initiation and Growth of Type 316 Stainless Steel." In ASME 2015 Pressure Vessels and Piping Conference. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/pvp2015-45812.
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This study was aimed at investigating the role of crack initiation and growth rate on the fatigue life reduction by environmental effect. First, crack length and the number of cracks were observed on the inner surface of specimens after fatigue test in PWR environment and air. Next, incubation time was deduced by inverse analysis. Third, statistical crack initiation and growth behavior was simulated by a Monte Carlo model. The influence of multiple crack interaction and coalescence to the fatigue life were discussed. It was revealed that environmental effect enhanced crack initiation and accelerated crack growth. Moreover, coalescence of cracks was estimated to influence fatigue life of 316 stainless steel in PWR environment.
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Yu, Yue, Zhanming Li, and Jinming Pan. "Effects of Exposing Eggs to Various Green Light Intensities during Incubation on Hatch Performance, Post-Hatch Grow Performance, Fear and Stress Responses in Layer Chicks." In 10th International Livestock Environment Symposium (ILES X). St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2018. http://dx.doi.org/10.13031/iles.18-062.
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Fitriani, Mia, Achmad Arifiyanto, Sumardi, Martha Lulus Lande, Christina Nugroho Ekowati, Titik Nur Aeny, and Hapin Afriyani. "Streptomyces hygroscopicus subspecies hygroscopicus strain I18: Incubation time and tryptophan concentration effects on Indole-3-acetic acid (IAA) hormone production." In THE 2ND UNIVERSITAS LAMPUNG INTERNATIONAL CONFERENCE ON SCIENCE, TECHNOLOGY, AND ENVIRONMENT (ULICoSTE) 2021. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0103220.
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Yasuda, Kyono, Nobuyuki Ishikawa, Yutaka Matsui, Daisuke Mizuno, and Tomoyuki Yokota. "In-Situ Investigation of Initiation and Propagation Behavior of Bainitic Steels in Sour Environment." In ASME 2018 37th International Conference on Ocean, Offshore and Arctic Engineering. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/omae2018-77471.
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Hydrogen induced cracking (HIC) occurs by the poisoning effect of hydrogen sulfide (H2S) which promotes hydrogen absorption and entry at steel surface. Therefore, it is important for linepipe steels to have sufficient HIC resistance in sour environments. The HIC resistance is usually evaluated by measuring cracks after the standardized immersion test such as NACE TM0284. However, the general evaluation method cannot investigate HIC initiation and propagation behavior separately. It is necessary to understand the effect of metallurgical factors on the cracking behavior of sour service linepipe. In this study, in-situ ultrasonic inspection equipment was applied to the HIC test for the several linepipe steels with bainitic microstructure in order to clarify crack initiation and propagation behavior quantitatively. The three dimensional (3-D) distribution of cracks in the specimen was successfully captured as time sequence, and the temporal change of the crack area ratio (CAR) was investigated. It was revealed that the CAR-time curves are consist of four stages with different CAR increment rate. The first stage is the incubation of crack initiation. In the second stage, cracks occur and grow, and adjacent cracks coalesced rapidly. Regarding the first and second stages, sensitivity for the HIC initiation was well correlated with the hydrogen diffusion coefficient and the density of crack initiation site, such as MnS and Nb inclusions. In the third stage, the coalesced cracks propagate along the center segregation region. From the investigation of individual crack behavior, the crack along harder region showed higher propagation rate. In the fourth stage, the crack propagation rate was decreased to be in stasis. It can be stated that crack growth in the final stage is strongly affected by the hardness of base material and the crack easily propagate when HIC occurs in high strength steels.
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Ngiap, Hio Chon, Ian A. Watson, Ruikang K. Wang, and Glenn Ward. "Optimising image processing systems to accurately count colony forming units." In The European Conference on Lasers and Electro-Optics. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/cleo_europe.1996.cthq5.
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Historically, the rate of experimentation involving micro biological procedures is slow due to, for example, the incubation and analysis of samples. Recently, a number of optical and image processing techniques have been developed to analyse samples to improve accuracy by removing human error and speed up the data acquisition process. One such example where commercial products (Data Cell, Berks. U.K.) are available is in counting colony forming units (efu) grown on agar culture plates. Commercial products are prohibitively expensive and, in general, do not support other applications of the image processing equipment. The present work developed macros to count Escherichia coli using OPTIMAS, a commercially available software package for general image processing applications. The effect of the environment - lighting conditions, camera, focusing, threshold intensity, and filtering - on the efficiency of the counting system was investigated.
Reports on the topic "Incubation environment"
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Weinberg, Zwi G., Richard E. Muck, Nathan Gollop, Gilad Ashbell, Paul J. Weimer, and Limin Kung, Jr. effect of lactic acid bacteria silage inoculants on the ruminal ecosystem, fiber digestibility and animal performance. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7587222.bard.
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The overall objective of the whole research was to elucidate the mechanisms by which LAB silage inoculants enhance ruminant performance. The results generated will permit the development of better silage inoculants that maximize both silage preservation and animal performance. For this one-year BARD feasibility study, the objectives were to: 1. determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF) 2.select the inoculants that survived best, and 3. test whether LAB silage inoculants produce bacteriocins-like substances. The most promising strains will be used in the next steps of the research. Silage inoculants containing LAB are used in order to improve forage preservation efficiency. In addition, silage inoculants enhance animal performance in many cases. This includes improvements in feed intake, liveweight gain and milk production in 25-40% of studies reviewed. The cause for the improvement in animal performance is not clear but appears to be other than direct effect of LAB inoculants on silage fermentation. Results from various studies suggest a possible probiotic effect. Our hypothesis is that specific LAB strains interact with rumen microorganisms which results in enhanced rumen functionality and animal performance. The first step of the research is to determine whether LAB of silage inoculants survive in RF. Silage inoculants (12 in the U.S. and 10 in Israel) were added to clarified and strained RF. Inoculation rate was 10 ⁶ (clarified RF), 10⁷ (strained RF) (in the U.S.) and 10⁷, 10⁸ CFU ml⁻¹ in Israel (strained RF). The inoculated RF was incubated for 72 and 96 h at 39°C, with and without 5 g 1⁻¹ glucose. Changes in pH, LAB numbers and fermentation products were monitored throughout the incubation period. The results indicated that LAB silage inoculants can survive in RF. The inoculants with the highest counts after 72 h incubation in rumen fluid were Lactobacillus plantarum MTD1 and a L. plantarum/P. cerevisiae mixture (USA) and Enterococcus faecium strains and Lactobacillus buchneri (Israel). Incubation of rumen fluid with silage LAB inoculants resulted in higher pH values in most cases as compared with that of un-inoculated controls. The magnitude of the effect varied among inoculants and typically was enhanced with the inoculants that survived best. This might suggest the mode of action of LAB silage inoculants in the rumen as higher pH enhances fibrolytic microorganisms in the rumen. Volatile fatty acid (VFA) concentrations in the inoculated RF tended to be lower than in the control RF after incubation. However, L. plalltarull1 MTDI resulted in the highest concentrations of VFA in the RF relative to other inoculants. The implication of this result is not as yet clear. In previous research by others, feeding silages which were inoculated with this strain consistently enhanced animal performance. These finding were recently published in Weinberg et.al.. (2003), J. of Applied Microbiology 94:1066-1071 and in Weinberg et al.. (2003), Applied Biochemistry and Biotechnology (accepted). In addition, some strains in our studies have shown bacteriocins like activity. These included Pediococcus pentosaceus, Enterococcus faecium and Lactobacillus plantarum Mill 1. These results will enable us to continue the research with the LAB strains that survived best in the rumen fluid and have the highest potential to affect the rumen environment.
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Jiang, Yanyao. Environmental Effects on the Incubation Time Characteristics in Stress-Corrosion Cracking. Fort Belvoir, VA: Defense Technical Information Center, April 2011. http://dx.doi.org/10.21236/ada553752.
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Borch, Thomas, Yitzhak Hadar, and Tamara Polubesova. Environmental fate of antiepileptic drugs and their metabolites: Biodegradation, complexation, and photodegradation. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597927.bard.
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Many pharmaceutical compounds are active at very low doses, and a portion of them regularly enters municipal sewage systems and wastewater-treatment plants following use, where they often do not fully degrade. Two such compounds, CBZ and LTG, have been detected in wastewater effluents, surface waters, drinking water, and irrigation water, where they pose a risk to the environment and the food supply. These compounds are expected to interact with organic matter in the environment, but little is known about the effect of such interactions on their environmental fate and transport. The original objectives of our research, as defined in the approved proposal, were to: Determine the rates, mechanisms and products of photodegradation of LTG, CBZ and selected metabolites in waters exposed to near UV light, and the influence of DOM type and binding processes on photodegradation. Determine the potential and pathways for biodegradation of LTG, CBZ and selected metabolites using a white rot fungus (Pleurotusostreatus) and ADP, and reveal the effect of DOM complexation on these processes. Reveal the major mechanisms of binding of LTG, CBZ and selected metabolites to DOM and soil in the presence of DOM, and evaluate the effect of this binding on their photodegradation and/or biodegradation. We determined that LTG undergoes relatively slow photodegradation when exposed to UV light, and that pH affects each of LTG’s ability to absorb UV light, the efficiency of the resulting reaction, and the identities of LTG’sphotoproducts (t½ = 230 to 500 h during summer at latitude 40 °N). We observed that LTG’sphotodegradation is enhanced in the presence of DOM, and hypothesized that LTG undergoes direct reactions with DOM components through nucleophilic substitution reactions. In combination, these data suggest that LTG’s fate and transport in surface waters are controlled by environmental conditions that vary with time and location, potentially affecting the environment and irrigation waters. We determined that P. ostreatusgrows faster in a rich liquid medium (glucose peptone) than on a natural lignocellulosic substrate (cotton stalks) under SSF conditions, but that the overall CBZ removal rate was similar in both media. Different and more varied transformation products formed in the solid state culture, and we hypothesized that CBZ degradation would proceed further when P. ostreatusand the ᵉⁿᶻʸᵐᵃᵗⁱᶜ ᵖʳᵒᶠⁱˡᵉ ʷᵉʳᵉ ᵗᵘⁿᵉᵈ ᵗᵒ ˡⁱᵍⁿⁱⁿ ᵈᵉᵍʳᵃᵈᵃᵗⁱᵒⁿ. ᵂᵉ ᵒᵇˢᵉʳᵛᵉᵈ ¹⁴C⁻Cᴼ2 ʳᵉˡᵉᵃˢᵉ ʷʰᵉⁿ ¹⁴C⁻ᶜᵃʳᵇᵒⁿʸˡ⁻ labeled CBZ was used as the substrate in the solid state culture (17.4% of the initial radioactivity after 63 days of incubation), but could not conclude that mineralization had occurred. In comparison, we determined that LTG does not degrade in agricultural soils irrigated with treated wastewater, but that P. ostreatusremoves up to 70% of LTG in a glucose peptone medium. We detected various metabolites, including N-oxides and glycosides, but are still working to determine the degradation pathway. In combination, these data suggest that P. ostreatuscould be an innovative and effective tool for CBZ and LTG remediation in the environment and in wastewater used for irrigation. In batch experiments, we determined that the sorption of LTG, CBZ and selected metabolites to agricultural soils was governed mainly by SOM levels. In lysimeter experiments, we also observed LTG and CBZ accumulation in top soil layers enriched with organic matter. However, we detected CBZ and one of its metabolites in rain-fed wheat previously irrigated with treated wastewater, suggesting that their sorption was reversible, and indicating the potential for plant uptake and leaching. Finally, we used macroscale analyses (including adsorption/desorption trials and resin-based separations) with molecular- level characterization by FT-ICR MS to demonstrate the adsorptive fractionation of DOM from composted biosolids by mineral soil. This suggests that changes in soil and organic matter types will influence the extent of LTG and CBZ sorption to agricultural soils, as well as the potential for plant uptake and leaching.
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El Halawani, Mohamed, and Israel Rozenboim. Environmental factors affecting the decline in reproductive efficiency of turkey hens: Mediation by vasoactive intestinal peptide. United States Department of Agriculture, January 2007. http://dx.doi.org/10.32747/2007.7696508.bard.
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Reproductive failure associated with heat stress is a well known phenomenon in avian species. Increased prolactin (PRL) levels in response to heat stress have been suggested as a mechanism involved in this reproductive malfunction. To test this hypothesis, laying female turkeys were subjected to 40°C for 12 h during the photo-phase daily or maintained at 24–26°C. Birds in each group received oral treatment with parachlorophenyalanine (PCPA; 50 mg/kg BW/day for 3 days), an inhibitor of serotonin (5-HT) biosynthesis; or immunized against vasoactive intestinal peptide (VIP). Both treatments are known to reduce circulating PRL levels. Non treated birds were included as controls. In the control group, high ambient temperature terminated egg laying, induced ovarian regression, reduced plasma luteinizing hormone (LH) and ovarian steroids (progesterone, testosterone, estradiol) levels, and increased plasma PRL levels and the incidence of incubation behavior. Pretreatment with PCPA reduced (P< 0.05) heat stress-induced decline in egg production, increase in PRL levels, and expression of incubation behavior. Plasma LH and ovarian steroid levels of heat stressed birds were restored to that of controls by PCPA treatment. As in PCPA-treated birds, VIP immunoneutralization of heat-stressed turkeys reduced (P< 0.05) circulating PRL levels and prevented the expression of incubation behavior. But it did not restore the decline in LH, ovarian steroids, and egg production (P> 0.05). The present findings indicate that the detrimental effect of high temperature on reproductive performance may not be related to the elevated PRL levels in heat-stressed birds but to mechanism(s) that involve 5-HT neurotransmission and the induction of hyperthermia.
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Venedicto, Melissa, and Cheng-Yu Lai. Facilitated Release of Doxorubicin from Biodegradable Mesoporous Silica Nanoparticles. Florida International University, October 2021. http://dx.doi.org/10.25148/mmeurs.009774.
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Cervical cancer is one of the most common causes of cancer death for women in the United States. The current treatment with chemotherapy drugs has significant side effects and may cause harm to healthy cells rather than cancer cells. In order to combat the potential side effects, nanoparticles composed of mesoporous silica were created to house the chemotherapy drug doxorubicin (DOX). The silica network contains the drug, and a pH study was conducted to determine the conditions for the nanoparticle to disperse the drug. The introduction of disulfide bonds within the nanoparticle created a framework to efficiently release 97% of DOX in acidic environments and 40% release in neutral environments. The denotation of acidic versus neutral environments was important as cancer cells are typically acidic. The chemistry was proved with the incubation of the loaded nanoparticle into HeLa cells for a cytotoxicity report and confocal imaging. The use of the framework for the anticancer drug was shown to be effective for the killing of cancerous cells.
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Yahav, Shlomo, John Brake, and Noam Meiri. Development of Strategic Pre-Natal Cycling Thermal Treatments to Improve Livability and Productivity of Heavy Broilers. United States Department of Agriculture, December 2013. http://dx.doi.org/10.32747/2013.7593395.bard.
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The necessity to improve broiler thermotolerance and live performance led to the following hypothesis: Appropriate comprehensive incubation treatments that include significant temperature management changes will promote angiogenesis and will improve acquisition of thermotolerance and carcass quality of heavy broilers through epigenetic adaptation. It was based on the following questions: 1. Can TM during embryogenesis of broilers induce a longer-lasting thermoregulatory memory (up to marketing age of 10 wk) that will improve acquisition of thermotolerance as well as increased breast meat yield in heavy broilers? 2. The improved sensible heat loss (SHL) suggests an improved peripheral vasodilation process. Does elevated temperature during incubation affect vasculogenesis and angiogenesis processes in the chick embryo? Will such create subsequent advantages for heavy broilers coping with adverse hot conditions? 3. What are the changes that occur in the PO/AH that induce the changes in the threshold response for heat production/heat loss based on the concept of epigenetic temperature adaptation? The original objectives of this study were as follow: a. to assess the improvement of thermotolerance efficiency and carcass quality of heavy broilers (~4 kg); b. toimproveperipheral vascularization and angiogenesis that improve sensible heat loss (SHL); c. to study the changes in the PO/AH thermoregulatory response for heat production/losscaused by modulating incubation temperature. To reach the goals: a. the effect of TM on performance and thermotolerance of broilers reared to 10 wk of age was studied. b. the effect of preincubation heating with an elevated temperature during the 1ˢᵗ 3 to 5 d of incubation in the presence of modified fresh air flow coupled with changes in turning frequency was elucidated; c.the effect of elevated temperature on vasculogenesis and angiogenesis was determined using in ovo and whole embryo chick culture as well as HIF-1α VEGF-α2 VEGF-R, FGF-2, and Gelatinase A (MMP2) gene expression. The effects on peripheral blood system of post-hatch chicks was determined with an infrared thermal imaging technique; c. the expression of BDNF was determined during the development of the thermal control set-point in the preoptic anterior hypothalamus (PO/AH). Background to the topic: Rapid growth rate has presented broiler chickens with seriousdifficulties when called upon to efficiently thermoregulate in hot environmental conditions. Being homeotherms, birds are able to maintain their body temperature (Tb) within a narrow range. An increase in Tb above the regulated range, as a result of exposure to environmental conditions and/or excessive metabolic heat production that often characterize broiler chickens, may lead to a potentially lethal cascade of irreversible thermoregulatory events. Exposure to temperature fluctuations during the perinatal period has been shown to lead to epigenetic temperature adaptation. The mechanism for this adaptation was based on the assumption that environmental factors, especially ambient temperature, have a strong influence on the determination of the “set-point” for physiological control systems during “critical developmental phases.” Recently, Piestunet al. (2008) demonstrated for the first time that TM (an elevated incubation temperature of 39.5°C for 12 h/d from E7 to E16) during the development/maturation of the hypothalamic-hypophyseal-thyroid axis (thermoregulation) and the hypothalamic-hypophyseal-adrenal axis (stress) significantly improved the thermotolerance and performance of broilers at 35 d of age. These phenomena raised two questions that were addressed in this project: 1. was it possible to detect changes leading to the determination of the “set point”; 2. Did TM have a similar long lasting effect (up to 70 d of age)? 3. Did other TM combinations (pre-heating and heating during the 1ˢᵗ 3 to 5 d of incubation) coupled with changes in turning frequency have any performance effect? The improved thermotolerance resulted mainly from an efficient capacity to reduce heat production and the level of stress that coincided with an increase in SHL (Piestunet al., 2008; 2009). The increase in SHL (Piestunet al., 2009) suggested an additional positive effect of TM on vasculogenesis and angiogensis. 4. In order to sustain or even improve broiler performance, TM during the period of the chorioallantoic membrane development was thought to increase vasculogenesis and angiogenesis providing better vasodilatation and by that SHL post-hatch.
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Yahav, Shlomo, John Brake, and Orna Halevy. Pre-natal Epigenetic Adaptation to Improve Thermotolerance Acquisition and Performance of Fast-growing Meat-type Chickens. United States Department of Agriculture, September 2009. http://dx.doi.org/10.32747/2009.7592120.bard.
Full textAbstract:
: The necessity to improve broiler thermotolerance and performance led to the following hypothesis: (a) thethermoregulatory-response threshold for heat production can be altered by thermal manipulation (TM) during incubation so as to improve the acquisition of thermotolerance in the post-hatch broiler;and (b) TM during embryogenesis will improve myoblast proliferation during the embryonic and post-hatch periods with subsequent enhanced muscle growth and meat production. The original objectives of this study were as follow: 1. to assess the timing, temperature, duration, and turning frequency required for optimal TM during embryogenesis; 2. to evaluate the effect of TM during embryogenesis on thermoregulation (heat production and heat dissipation) during four phases: (1) embryogenesis, (2) at hatch, (3) during growth, and (4) during heat challenge near marketing age; 3. to investigate the stimulatory effect of thermotolerance on hormones that regulate thermogenesis and stress (T₄, T₃, corticosterone, glucagon); 4. to determine the effect of TM on performance (BW gain, feed intake, feed efficiency, carcass yield, breast muscle yield) of broiler chickens; and 5. to study the effect of TM during embryogenesis on skeletal muscle growth, including myoblast proliferation and fiber development, in the embryo and post-hatch chicks.This study has achieved all the original objectives. Only the plasma glucagon concentration (objective 3) was not measured as a result of technical obstacles. Background to the topic: Rapid growth rate has presented broiler chickens with seriousdifficulties when called upon to efficiently thermoregulate in hot environmental conditions. Being homeotherms, birds are able to maintain their body temperature (Tb) within a narrow range. An increase in Tb above the regulated range, as a result of exposure to environmental conditions and/or excessive metabolic heat production that often characterize broiler chickens, may lead to a potentially lethal cascade of irreversible thermoregulatory events. Exposure to temperature fluctuations during the perinatal period has been shown to lead to epigenetic temperature adaptation. The mechanism for this adaptation was based on the assumption that environmental factors, especially ambient temperature, have a strong influence on the determination of the “set-point” for physiological control systems during “critical developmental phases.” In order to sustain or even improve broiler performance, TM during the period of embryogenesis when satellite cell population normally expand should increase absolute pectoralis muscle weight in broilers post-hatch. Major conclusions: Intermittent TM (39.5°C for 12 h/day) during embryogenesis when the thyroid and adrenal axis was developing and maturing (E7 to E16 inclusive) had a long lasting thermoregulatory effect that improved thermotolerance of broiler chickens exposed to acute thermal stress at market age by lowering their functional Tb set point, thus lowering metabolic rate at hatch, improving sensible heat loss, and significantly decreasing the level of stress. Increased machine ventilation rate was required during TM so as to supply the oxygen required for the periods of increased embryonic development. Enhancing embryonic development was found to be accomplished by a combination of pre-incubation heating of embryos for 12 h at 30°C, followed by increasing incubation temperature to 38°C during the first 3 days of incubation. It was further facilitated by increasing turning frequency of the eggs to 48 or 96 times daily. TM during critical phases of muscle development in the late-term chick embryo (E16 to E18) for 3 or 6 hours (39.5°C) had an immediate stimulatory effect on myoblast proliferation that lasted for up to two weeks post-hatch; this was followed by increased hypertrophy at later ages. The various incubation temperatures and TM durations focused on the fine-tuning of muscle development and growth processes during late-term embryogenesis as well as in post-hatch chickens.
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Shenker, Moshe, Paul R. Bloom, Abraham Shaviv, Adina Paytan, Barbara J. Cade-Menun, Yona Chen, and Jorge Tarchitzky. Fate of Phosphorus Originated from Treated Wastewater and Biosolids in Soils: Speciation, Transport, and Accumulation. United States Department of Agriculture, June 2011. http://dx.doi.org/10.32747/2011.7697103.bard.
Full textAbstract:
Beneficial use of reclaimed wastewater (RW) and biosolids (BS) in soils is accompanied by large input of sewage-originated P. Prolonged application may result in P accumulation up to levelsBeneficial use of reclaimed wastewater (RW) and biosolids (BS) in soils is accompanied by large input of sewage-originated P. Prolonged application may result in P accumulation up to levels that impair plant nutrition, increase P loss, and promote eutrophication in downstream waters. This study aims to shed light on the RW- and BS-P forms in soils and to follow the processes that determine P reactivity, solubility, availability, and loss in RW and BS treated soils. The Technion group used sequential P extraction combined with measuring stable oxygen isotopic composition in phosphate (δ18OP) and with 31P-NMR studies to probe P speciation and transformations in soils irrigated with RW or fresh water (FW). The application of the δ18OP method to probe inorganic P (Pi) speciation and transformations in soils was developed through collaboration between the Technion and the UCSC groups. The method was used to trace Pi in water-, NaHCO3-, NaOH-, and HCl- P fractions in a calcareous clay soil (Acre, Israel) irrigated with RW or FW. The δ18OP signature changes during a month of incubation indicated biogeochemical processes. The water soluble Pi (WSPi) was affected by enzymatic activity yielding isotopic equilibrium with the water molecules in the soil solution. Further it interacted rapidly with the NaHCO3-Pi. The more stable Pi pools also exhibited isotopic alterations in the first two weeks after P application, likely related to microbial activity. Isotopic depletion which could result from organic P (PO) mineralization was followed by enrichment which may result from biologic discrimination in the uptake. Similar transformations were observed in both soils although transformations related to biological activity were more pronounced in the soil treated with RW. Specific P compounds were identified by the Technion group, using solution-state 31P-NMR in wastewater and in soil P extracts from Acre soils irrigated by RW and FW. Few identified PO compounds (e.g., D-glucose-6-phosphate) indicated coupled transformations of P and C in the wastewater. The RW soil retained higher P content, mainly in the labile fractions, but lower labile PO, than the FW soil; this and the fact that P species in the various soil extracts of the RW soil appear independent of P species in the RW are attributed to enhanced biological activity and P recycling in the RW soil. Consistent with that, both soils retained very similar P species in the soil pools. The HUJ group tested P stabilization to maximize the environmental safe application rates and the agronomic beneficial use of BS. Sequential P extraction indicated that the most reactive BS-P forms: WSP, membrane-P, and NaHCO3-P, were effectively stabilized by ferrous sulfate (FeSul), calcium oxide (CaO), or aluminum sulfate (alum). After applying the stabilized BS, or fresh BS (FBS), FBS compost (BSC), or P fertilizer (KH2PO4) to an alluvial soil, P availability was probed during 100 days of incubation. A plant-based bioassay indicated that P availability followed the order KH2PO4 >> alum-BS > BSC ≥ FBS > CaO-BS >> FeSul-BS. The WSPi concentration in soil increased following FBS or BSC application, and P mineralization further increased it during incubation. In contrast, the chemically stabilized BS reduced WSPi concentrations relative to the untreated soil. It was concluded that the chemically stabilized BS effectively controlled WSPi in the soil while still supplying P to support plant growth. Using the sequential extraction procedure the persistence of P availability in BS treated soils was shown to be of a long-term nature. 15 years after the last BS application to MN soils that were annually amended for 20 years by heavy rates of BS, about 25% of the added BS-P was found in the labile fractions. The UMN group further probed soil-P speciation in these soils by bulk and micro X-ray absorption near edge structure (XANES). This newly developed method was shown to be a powerful tool for P speciation in soils. In a control soil (no BS added), 54% of the total P was PO and it was mostly identified as phytic acid; 15% was identified as brushite and 26% as strengite. A corn crop BS amended soil included mostly P-Fe-peat complex, variscite and Al-P-peat complex but no Ca-P while in a BS-grass soil octacalcium phosphate was identified and o-phosphorylethanolamine or phytic acid was shown to dominate the PO fraction that impair plant nutrition, increase P loss, and promote eutrophication in downstream waters. This study aims to shed light on the RW- and BS-P forms in soils and to follow the processes that determine P reactivity, solubility, availability, and loss in RW and BS treated soils. The Technion group used sequential P extraction combined with measuring stable oxygen isotopic composition in phosphate (δ18OP) and with 31P-NMR studies to probe P speciation and transformations in soils irrigated with RW or fresh water (FW). The application of the δ18OP method to probe inorganic P (Pi) speciation and transformations in soils was developed through collaboration between the Technion and the UCSC groups. The method was used to trace Pi in water-, NaHCO3-, NaOH-, and HCl- P fractions in a calcareous clay soil (Acre, Israel) irrigated with RW or FW. The δ18OP signature changes during a month of incubation indicated biogeochemical processes. The water soluble Pi (WSPi) was affected by enzymatic activity yielding isotopic equilibrium with the water molecules in the soil solution. Further it interacted rapidly with the NaHCO3-Pi. The more stable Pi pools also exhibited isotopic alterations in the first two weeks after P application, likely related to microbial activity. Isotopic depletion which could result from organic P (PO) mineralization was followed by enrichment which may result from biologic discrimination in the uptake. Similar transformations were observed in both soils although transformations related to biological activity were more pronounced in the soil treated with RW. Specific P compounds were identified by the Technion group, using solution-state 31P-NMR in wastewater and in soil P extracts from Acre soils irrigated by RW and FW. Few identified PO compounds (e.g., D-glucose-6-phosphate) indicated coupled transformations of P and C in the wastewater. The RW soil retained higher P content, mainly in the labile fractions, but lower labile PO, than the FW soil; this and the fact that P species in the various soil extracts of the RW soil appear independent of P species in the RW are attributed to enhanced biological activity and P recycling in the RW soil. Consistent with that, both soils retained very similar P species in the soil pools. The HUJ group tested P stabilization to maximize the environmental safe application rates and the agronomic beneficial use of BS. Sequential P extraction indicated that the most reactive BS-P forms: WSP, membrane-P, and NaHCO3-P, were effectively stabilized by ferrous sulfate (FeSul), calcium oxide (CaO), or aluminum sulfate (alum). After applying the stabilized BS, or fresh BS (FBS), FBS compost (BSC), or P fertilizer (KH2PO4) to an alluvial soil, P availability was probed during 100 days of incubation. A plant-based bioassay indicated that P availability followed the order KH2PO4 >> alum-BS > BSC ≥ FBS > CaO-BS >> FeSul-BS. The WSPi concentration in soil increased following FBS or BSC application, and P mineralization further increased it during incubation. In contrast, the chemically stabilized BS reduced WSPi concentrations relative to the untreated soil. It was concluded that the chemically stabilized BS effectively controlled WSPi in the soil while still supplying P to support plant growth. Using the sequential extraction procedure the persistence of P availability in BS treated soils was shown to be of a long-term nature. 15 years after the last BS application to MN soils that were annually amended for 20 years by heavy rates of BS, about 25% of the added BS-P was found in the labile fractions. The UMN group further probed soil-P speciation in these soils by bulk and micro X-ray absorption near edge structure (XANES). This newly developed method was shown to be a powerful tool for P speciation in soils. In a control soil (no BS added), 54% of the total P was PO and it was mostly identified as phytic acid; 15% was identified as brushite and 26% as strengite. A corn crop BS amended soil included mostly P-Fe-peat complex, variscite and Al-P-peat complex but no Ca-P while in a BS-grass soil octacalcium phosphate was identified and o-phosphorylethanolamine or phytic acid was shown to dominate the PO fraction.
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El Halawani, Mohamed, and Israel Rozenboim. Temperature Stress and Turkey Reproduction. United States Department of Agriculture, May 2002. http://dx.doi.org/10.32747/2002.7570546.bard.
Full textAbstract:
High temperature stress is of major concern to turkey producers in Israel and the United States. The decline in the rate of egg production at high environmental temperature is well recognized, but the neuroendocrinological basis is not understood. Our objectives were: 1) to characterize the hypothalamo-hypophyseal axis involvement in the mechanism(s) underlying the detrimental effect of heat stress on reproduction, and 2) to establish procedures that alleviate the damaging effect of heat stress on reproduction. Heat stress (40oC, Israel; 32oC, U.S.) caused significant reduction in egg production, which was restored by VIP immunoneutralization. The decline in egg production did not appear to be entirely related to the expression of incubation behavior due to the rise in circulating PRL in stressed birds. Heat stress was found to increase circulating PRL in ovariectomized turkeys independent of the reproductive stage. Active immunization against VIP was shown for the first time to up-regulate LHb and FSHb subunit mRNA contents. These findings taken together with the results that the heat stress-induced decline in egg production may not be dependent upon the reproductive stage, lead to the suggestion that the detrimental effect of heat stress on reproductive performance may be in part mediated by VIP acting directly on the GnRH/gonadotropin system. Inhibin (INH) immunoneutralization has been shown to enhance FSH secretion and induces ovulation in mammals. It is hypothesized that immunization of heat-stressed turkeys against INH will increase levels of circulating FSH and the number of preovulating follicles which leads to improved reproductive performance. We have cloned and expressed turkey INH-a and INH-bA. Active immunization of turkey hens with rtINH-a increased pituitary FSH-b subunit mRNA and the number of non-graded preovulatory yellow follicles, but no significant increase in egg production was observed.