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Journal articles on the topic 'Inoculation Technique'

Author: Grafiati

Published: 5 June 2025

Last updated: 15 July 2025

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1

Driskel, Barbara A., Robert M. Hunger, Mark E. Payton, and Jeanmarie Verchot-Lubicz. "Response of Hard Red Winter Wheat to Soilborne wheat mosaic virus Using Novel Inoculation Methods." Phytopathology® 92, no. 4 (2002): 347–54. http://dx.doi.org/10.1094/phyto.2002.92.4.347.

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Soilborne wheat mosaic virus (SBWMV) is an agronomically important pathogen of wheat that is transmitted by the soilborne plasmodiophorid vector Polymyxa graminis. In the laboratory, attempts to generate SBWMV-infected plants are often hampered by poor infectivity of the virus. To analyze the mechanism for virus resistance in wheat cultivars, we developed novel inoculation techniques. A new technique for foliar inoculation of SBWMV was developed that eliminated wound-induced necrosis normally associated with rub inoculating virus to wheat leaves. This new technique is important because we can

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2

Sun, G. C., S. Y. Sun, and Z. T. Shen. "A New Inoculation Technique for Rice Blast (Bl)." International Rice Research Newsletter 14, no. 2 (1989): 15. https://doi.org/10.5281/zenodo.6997513.

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This article 'A New Inoculation Technique for Rice Blast (Bl)' appeared in the International Rice Research Newsletter series, created by the International Rice Research Institute (IRRI). The primary objective of this publication was to expedite communication among scientists concerned with the development of improved technology for rice and for rice based cropping systems. This publication will report what scientists are doing to increase the production of rice in as much as this crop feeds the most densely populated and land scarce nations in the world.

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3

Kaur, Yesmin, J. S. Lore, and P. P. S. Pannu. "Development of screening technique for artificial creation of false smut in rice." Journal of Applied and Natural Science 10, no. 1 (2018): 253–57. http://dx.doi.org/10.31018/jans.v10i1.1613.

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False smut (Ustilaginoidea virens (Cooke) Takahashi) of rice is an emerging disease and a potential threat to rice growers in different countries due to reduction in yield, quality and production of mycotoxins. Development and cultivation of resistant varieties is desirable approach for its management. Different inoculation techniques viz. spray inoculation, syringe inoculation and dusting method were evaluated for creating artificial epiphytotic conditions in susceptible variety PR116 under natural as well as artificially provided humidity conditions. Spray inoculation method produced more nu

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4

McNabb, W. M., C. G. J. van den Berg, and S. R. Rimmer. "Comparison of inoculation methods for selection of plants resistant to Leptosphaeria maculans in Brassica napus." Canadian Journal of Plant Science 73, no. 4 (1993): 1199–207. http://dx.doi.org/10.4141/cjps93-159.

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The relationship between ratings following four inoculation techniques and field ratings and the usefulness of these techniques for screening large populations was determined. Plants of Brassica napus ’Westar,’ ’Wesroona’, ’Topas’, ’Hanna’ and ’R8314’ were inoculated with L. maculans using four techniques: cotyledon inoculation with cotyledon rating, leaf inoculation with leaf and stem rating, stem inoculation with stem rating and inoculation using infested oat kernels with stem rating. Plants from each combination of cultivar and technique were self-pollinated for evaluation of disease rating

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Howard Ament, Malissa, Robert M. Augé, Larry F. Grand, and Mark T. Windham. "An Inoculation Technique for Dogwood Anthracnose." Journal of Environmental Horticulture 16, no. 1 (1998): 37–41. http://dx.doi.org/10.24266/0738-2898-16.1.37.

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Abstract Researchers have been generally unable to infect dogwood foliage (Cornus florida L.) with dogwood anthracnose (Discula destructiva Redlin) in artificial environments. We tested the influence of four factors on development of D. destructiva lesions in intact C. florida leaves wounded with a pin-prick device: (1) propagule (conidia or vegetative hyphae), (2) isolate origin (Catoctin Mountain, MD or Great Smoky Mountains National Park, TN), (3) controlled environment (growth room or air conditioned, humidified chamber in a greenhouse), and (4) period of time leaves were enclosed in humid

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Wahyuni, Resti, and Amalia Indah Prihantini. "The Best Inoculation Technique Applied on Gyrinops versteegii Tree Trunk." Biosaintifika: Journal of Biology & Biology Education 12, no. 2 (2020): 235–39. http://dx.doi.org/10.15294/biosaintifika.v12i2.25128.

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Agarwood is a non-timber forest product having high economic value, however, its population in nature is getting decrease. Gyrinops versteegii, an agarwood-producing plant, has been listed in Appendix II CITES, therefore, exploitation of the agarwood in nature must be reduced. An effort to reduce the agarwood exploitation the nature is by agarwood cultivation including cultivation of agarwood-producing plant and agarwood inoculation. Some of agarwood inoculation methods are simpori and implant techniques. Simpori is a modified inoculation method using porous nail and Fusarium solani, meanwhile

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Williams, W. Paul, Michael N. Alpe, Gary L. Windham, Seval Ozkan, and J. Erik Mylroie. "Comparison of Two Inoculation Methods for Evaluating Maize for Resistance toAspergillus flavusInfection and Aflatoxin Accumulation." International Journal of Agronomy 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/972316.

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Aflatoxin, the most potent carcinogen found in nature, is produced by the fungusAspergillus flavusand occurs naturally in maize,Zea maysL. Growing maize hybrids with genetic resistance to aflatoxin contamination are generally considered a highly desirable way to reduce losses to aflatoxin. Developing resistant hybrids requires reliable inoculation methods for screening maize germplasm for resistance toA. flavusinfection and aflatoxin accumulation. The side-needle technique is a widely used inoculation technique: anA. flavusconidial suspension is injected underneath the husks into the side of t

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8

Shokes, F. M., K. Róźalski, D. W. Gorbet, T. B. Brenneman, and D. A. Berger. "Techniques for Inoculation of Peanut with Sclerotium rolfsii in the Greenhouse and Field1." Peanut Science 23, no. 2 (1996): 124–28. http://dx.doi.org/10.3146/i0095-3679-23-2-11.

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Abstract Four greenhouse experiments and a field trial were conducted in 1994 to determine the most effective technique for inoculation of peanut (Arachis hypogaea L.) with Sclerotium rolfsii Sacc. The cultivar Florunner was grown in the greenhouse in 20-cm-diameter pots and inoculated 48 d after planting (DAP). Plants in the field experiment were grown in plots 2.7 m long on 0.9 m centers, thinned to 11 plants per plot, and alternate plants were flagged for inoculation at 48 DAP. Inoculation techniques for all experiments were (a) a germinating sclerotium on a 1-cm-diameter agar disk [potato

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9

Prajapati, Krupal V., Mitesh R. Prajapati, B. Megala Devi, et al. "Paper Towel Method: In Vitro Inoculation Technique for Rapid and Robust Assessment of Clusterbean and Cowpea Genotypes Against Macrophomina phaseolina." Microbiology Research 15, no. 4 (2024): 2522–34. https://doi.org/10.3390/microbiolres15040168.

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The development and standardization of an effective inoculation technique are essential for reliable screening of crop genotypes. In this study, three inoculation methods—in vitro paper towel, in vitro agar, and soil inoculation in pots—were tested to evaluate the pathogenic potential of Macrophomina phaseolina, the causative agent of dry root rot (DRR). Since the pathogenicity of M. phaseolina is influenced by environmental factors, identifying a robust inoculation method is critical. Among the three techniques, the paper towel inoculation method resulted in the highest seedling mortality of

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10

Schachner, Robert D., Tie Qian, Sheryl Strasser, Mathias D. Brendel, Rodolfo Alejandro, and Daniel H. Mintz. "A Minimally Invasive Technique for Intrathymic Cell Transplantation in the Dog." Cell Transplantation 3, no. 4 (1994): 349–50. http://dx.doi.org/10.1177/096368979400300413.

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As an alternative to drug immunosuppression, attempts at inducing donor-specific tolerance by intrathymic (IT) inoculations to transplant recipient of donor origin alloantigenic products has proven very promising. Using fiber optic thoracoscopy, a technique for the study of this phenomena was developed for the dog. We show an approach to the dog thymus using fiber optics for injection of bone marrow (BM) cells as the tolerogen. Bone marrow was retrieved from the donor beagles and purified using an automated Ficoll-Paque gradient technique. The purified cellular suspension was injected into the

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11

Sun, G. C., S. Y. Sun, and Z. T. Shen. "A New Inoculation Technique for Neck Blast (Bl) on in Vitro Rice Panicles." International Rice Research Newsletter 17, no. 6 (1992): 23–24. https://doi.org/10.5281/zenodo.7219139.

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This article 'A New Inoculation Technique for Neck Blast (Bl) on in Vitro Rice Panicles' appeared in the International Rice Research Newsletter series, created by the International Rice Research Institute (IRRI). The primary objective of this publication was to expedite communication among scientists concerned with the development of improved technology for rice and for rice based cropping systems. This publication will report what scientists are doing to increase the production of rice in as much as this crop feeds the most densely populated and land scarce nations in the world.

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Auclair, J., G. J. Boland, E. Cober, et al. "Development of a new field inoculation technique to assess partial resistance in soybean to Sclerotinia sclerotiorum." Canadian Journal of Plant Science 84, no. 1 (2004): 57–64. http://dx.doi.org/10.4141/p03-035.

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The use of appropriate techniques to assess disease resistance is crucial to understanding the genetic control of host resistance to pathogens to develop resistant plant varieties. The objective of this study was to develop and evaluate a novel field-based inoculation technique for sclerotinia stem rot in soybean [Glycine max (L.) Merr.], caused by Sclerotinia sclerotiorum (Lib.) de Bary. The barley kernel inoculation technique involves stem inoculation using an infected barley kernel inserted into a wound made in the soybean stem. The damage on soybean plants is measured by the length of the

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13

Kumar, Santosh, Md Nadeem Akhtar, Erayya, and Tribhuwan Kumar. "Standardization of inoculation techniques for sheath blight of rice caused by Rhizoctonia solani (Kuhn)." Bangladesh Journal of Botany 48, no. 4 (2019): 1107–13. http://dx.doi.org/10.3329/bjb.v48i4.49059.

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To develop a simple and reliable inoculation technique using inoculum sources viz., mycelial suspension, mycelial ball, sclerotia and soil inoculation with homogenized mycelia suspension was carried out. The efficiency of different inoculation techniques were tested on susceptible rice variety Rejendra Sweta. Sheath inoculation with sclerotia gave lesion length 10.33 and 12.33 cm after seven and 15 days of inoculation, respectively. It also shows more significance in terms of relative lesion height to plant height i.e. 30.06 % followed by soil inoculation with homogenized mycelial suspension i

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14

Li, Yinping, Suli Sun, Chao Zhong, and Zhendong Zhu. "Detached-petiole inoculation method to evaluate Phytophthora root rot resistance in soybean plants." Crop and Pasture Science 68, no. 6 (2017): 555. http://dx.doi.org/10.1071/cp17158.

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Phytophthora root rot (PRR) caused by Phytophthora sojae, is one of the most destructive soybean diseases. The deployment of resistant cultivars is an important disease management strategy. To this aim, the development of a fast and effective method to evaluate soybean resistance to P. sojae is strategic. In this study, a detached-petiole inoculation technique was developed and its reliability was verified in soybean cultivars and segregant populations for PRR resistance. The detached-petiole and hypocotyl inoculation methods were used to assess the resistance of soybean cultivars, the F2 popu

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15

Engle, Jessica S., Laurence V. Madden, and Patrick E. Lipps. "Evaluation of Inoculation Methods to Determine Resistance Reactions of Wheat to Fusarium graminearum." Plant Disease 87, no. 12 (2003): 1530–35. http://dx.doi.org/10.1094/pdis.2003.87.12.1530.

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Reliable greenhouse assays are needed to differentiate types of resistance in wheat to Fusarium graminearum. Genotypes with known field reactions were evaluated for resistance type using four greenhouse inoculation techniques. Percentage of spikelets with symptoms per spike (severity) and percentage of inoculated spikelets per spike developing symptoms were assessed 7, 10, and 14 days after inoculation (DAI). Genotypes were evaluated using disease assessments 14 DAI and area under the disease progress curve (AUDPC). Significant genotype-inoculation technique interactions for disease assessment

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16

Srianturi, Yanuari, and Elvia Purwaningrum Dyahpalupi. "Konseling Kelompok Inokulasi Stres untuk Mengurangi Kecemasan Akademik di Era Pandemi." Indonesian Journal of Educational Counseling 7, no. 1 (2023): 139–45. http://dx.doi.org/10.30653/001.202371.257.

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This study aims to test the effectiveness of group counseling with stress inoculation training techniques to reduce student school as research subjects who were selected using purposive sampling technique. The results showed that group counseling with the stress inoculation training technique was effective in reducing student anxiety in facing exams in the era of the covid-19 anxiety in facing exams in the era of the covid-19 pandemic. The design of this study used an experimental method with a pre-test – post test. Students' exam anxiety was measured by the Achievement Emotions Questionnaire

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17

Sarr, Papa Saliou, Judith Wase Okon, Didier Aime Boyogueno Begoude, et al. "Symbiotic N2-Fixation Estimated by the15N Tracer Technique and Growth ofPueraria phaseoloides(Roxb.) Benth. Inoculated withBradyrhizobiumStrain in Field Conditions." Scientifica 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/7026859.

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This field experiment was established in Eastern Cameroon to examine the effect of selected rhizobial inoculation on N2-fixation and growth ofPueraria phaseoloides. Treatments consisted of noninoculated andBradyrhizobium yuanmingenseS3-4-inoculatedPuerariawith three replications each.Ipomoea batatasas a non-N2-fixing reference was interspersed in eachPuerariaplot. All the twelve plots received 2 gN/m2of15N ammonium sulfate 10% atom excess. At harvest, dry matter yields and the nitrogen derived from atmospheric N2-fixation (%Ndfa) of inoculatedPuerariawere significantly (P<0.05) higher (81%

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Machineski, Gabriela S., Andrea S. Scaramal, Maria A. Matos, et al. "Agronomic Efficiency of Signum Inoculant in Pre-inoculation of Soybean at 35 and 20 Days Before Sowing in Treated Seeds." Journal of Agricultural Science 14, no. 6 (2022): 141. http://dx.doi.org/10.5539/jas.v14n6p141.

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Pre-inoculating soybean seeds can make sowing faster and provide additional benefits to farmers. However, it needs to guarantee the nitrogen supply to maintain the viability and sustainability of the technique. In this study, we evaluated the agronomic efficiency of SIGNUM&reg; inoculant in the pre-inoculation at 20 and 35 days before sowing chemically treated soybean seeds. Experiments were conducted in four field experiments located at Paran&aacute;, Brazil, with a history of soybean cultivation managed under no-tillage systems, with crop rotation according to regional edaphoclimatic

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Paul, I., D. P. Isore, S. N. Joardar, S. K. Mukhopadhayay, and S. Ganguly. "Pathogenicity test ofStaphylococcus sp.by experimental animal inoculation technique." Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 35, no. 2 (2014): 95. http://dx.doi.org/10.5958/0974-0147.2014.00013.0.

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Esteve, J. M., and A. F. B. Fracchia. "Inoculation against Stress: a technique for beginning teachers." European Journal of Teacher Education 9, no. 3 (1986): 261–69. http://dx.doi.org/10.1080/0261976860090307.

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Porto, Lis Natali Rodrigues, and Alfredo Seiiti Urashima. "Development of a single uredinium inoculation method for Puccinia kuehnii, the causal agent of sugarcane orange rust." Summa Phytopathologica 44, no. 4 (2018): 311–16. http://dx.doi.org/10.1590/0100-5405/181427.

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ABSTRACT The use of resistant varieties is the ideal method to control rusts. Nevertheless, knowing the pathogen’s diversity is fundamental to the success of this measure. Diversity can be analyzed phenotypically and/or genotypically. For phenotypic diversity, the reaction of genotypes is assessed by means of inoculations of the pathogen generally obtained from several uredinia. One handicap of this technique is its impossibility to detect diversity among these uredinia, assuming that they are all homogenous. Therefore, the aim of the present study was to develop a single uredinium technique f

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Butt, Kevin R., James Frederickson, and Richard M. Morris. "The Earthworm Inoculation Unit technique: An integrated system for cultivation and soil-inoculation of earthworms." Soil Biology and Biochemistry 29, no. 3-4 (1997): 251–57. http://dx.doi.org/10.1016/s0038-0717(96)00053-3.

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23

Sood, Sushma G., Jack C. Comstock, and Neil C. Glynn. "Leaf Whorl Inoculation Method for Screening Sugarcane Rust Resistance." Plant Disease 93, no. 12 (2009): 1335–40. http://dx.doi.org/10.1094/pdis-93-12-1335.

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Brown rust, caused by Puccinia melanocephala, and orange rust, caused by P. kuehnii, are agronomically important diseases of sugarcane in Florida. Cultivar resistance is the best means of controlling these diseases. Natural infection has been the primary means of assessing resistance in sugarcane cultivars against rusts; unfortunately, natural infection is not always efficient in identifying resistant cultivars due to variable environmental conditions. Therefore, a more reliable screening method is needed to effectively select resistant genotypes. An inoculation technique was evaluated for ide

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Clements, M. J., C. E. Kleinschmidt, C. M. Maragos, J. K. Pataky, and D. G. White. "Evaluation of Inoculation Techniques for Fusarium Ear Rot and Fumonisin Contamination of Corn." Plant Disease 87, no. 2 (2003): 147–53. http://dx.doi.org/10.1094/pdis.2003.87.2.147.

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Fumonisins have been associated with potentially serious toxicoses of animals and humans. Prior to initiating a corn (Zea mays) breeding program for resistance to these mycotoxins, an efficient inoculation technique must be developed. Four inoculation techniques were evaluated on 14 commercial corn hybrids in Urbana, IL in 1999 and 2000. The techniques were: injection of inoculum through the ear husk leaves at R2 (blister); silks sprayed with inoculum at R2 and covered with a shoot bag until harvest; silks sprayed with inoculum at R2, covered with a shoot bag, reinoculated 1 week thereafter, a

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Susilo, F. X., Nyoman Mulyasari, Rozali Rozali, and Sudi Pramono. "HOST MORTALITY, LETHAL PERIOD, TRANSMISSIBILITY, AND THRESHOLD DENSITY OF VERTICILLIUM LECANII – SCOTINOPHARA SP. MYCOSIS." Jurnal Hama dan Penyakit Tumbuhan Tropika 5, no. 2 (2005): 88–96. http://dx.doi.org/10.23960/j.hptt.2588-96.

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Host mortality, lethal period, transmissibility, and threshold density of Verticillium lecanii – Scotinophara sp. mycosis. This study, conducted at Laboratory of Plant Protection Gading Rejo, Tanggamus-Lampung during March – October 2002, was aimed at determining the host mortality, lethal period, transmissibility, and threshold density of Verticillium lecanii – Scotinophara sp. mycosis generated from cadaver exposure and conidial spray in potted rice plants. The cadaver exposure was implemented by inoculating various levels of inoculum to different levels of host population while the conidial

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Rawana, Rawana, S. Hardiwinoto, Budiadi Budiadi, S. Rahayu, and A. Prijono. "The Effect of Fusarium Fungal Inoculation, Hole Position, and Induction Technique on Forming Agarwood in Gyrinops versteegii Tree." Jurnal Manajemen Hutan Tropika (Journal of Tropical Forest Management) 30, no. 3 (2024): 314–25. https://doi.org/10.7226/jtfm.30.3.314.

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Gyrinops versteegii, belonging to the Themelaeaceae family, is one of the species producing high-grade agarwood. The induction technique can accelerate the agarwood product. This study inducted the G. versteegii tree with fungal species, a variation of hole position, and an induction technique. The research was done at a domesticated G. versteegii plantation in Sragen and Karanganyar District, Central Java Province. The research material was 18 agarwood trees aged 14 years. Initial inoculation was carried out at a depth of 50 cm from the ground, inoculation holes were made vertically every 50

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Hasanah, Irja Trifirdatun, and Nurjannah. "Teknik Stress Inoculation Training Prespektif Islam." Jurnal al-Shifa Bimbingan Konseling Islam 3, no. 2 (2022): 88–95. http://dx.doi.org/10.32678/alshifa.v3i2.7532.

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This study discusses the Stress Inoculation Training(SIT) technique which was developed and evaluated to be come Islamic-based guidance and counselling in practice. The purpose of guidance and counselling is to help a person to find a solution to his psychological problems with both preventive and treatment approaches. Through guidance and counseling, counsellors can help individuals to deal with various problems including family, school, work and community problems. This study uses the method of literature study. The source of the data used in this study is secondary data or data obtained not

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Pazdernik, D. L., G. L. Hartman, Y. H. Huang, and T. Hymowitz. "A Greenhouse Technique for Assessing Phytophthora Root Rot Resistance in Glycine max and G. soja." Plant Disease 81, no. 10 (1997): 1112–14. http://dx.doi.org/10.1094/pdis.1997.81.10.1112.

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New sources of soybean (Glycine max) resistance to Phytophthora sojae are needed to provide effective resistance because of the rapidly changing race patterns of P. sojae in fields. The objectives of our study were to develop a method to screen Glycine soja for resistance to P. sojae and then use this methodology to screen G. soja lines for resistance to P. sojae races 1, 3, and 20. An agar plug-inoculation method, in which a 3-mm-diameter mycelial plug of the fungus was placed mycelium side down on cotyledons of 10-day-old soybean seedlings, was directly compared with the traditional hypocoty

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Barros, Luiz Henrique da Silva, Marcio Osvaldo Lima Magalhães, Alessandro Bandeira Dalbianco, Rodrigo Ribeiro Fidelis, Hugo Henrique Ribeiro Rosa, and Roberto Antonio Savelli Martinez. "Soybean inoculation techniques for the region of Tangará da Serra - MT, Brazil." Research, Society and Development 9, no. 8 (2020): e724986029. http://dx.doi.org/10.33448/rsd-v9i8.6029.

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The use of inoculated microorganisms on seeds has increased in Brazil, as they help on the fixation and use of nutrients by plants, promoting increasing yields, mainly due to the reduction in the application of nitrogen fertilizers. The aim of this study was to evaluate the influence of single inoculation and co-inoculation of Azospirillum brasilense and Bradyrhizobium japonicum bacteria on seeds and foliar inoculation on soybean, in the region of Tangará da Serra – MT, Brazil. The experimental design used was a randomized block with 9 treatments and 4 replicates. The treatments were composed

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Geremew, Dereje, Tadale Shiberu, and Ararsa Leta. "Evaluation of endophytic colonization and establishment of entomopathogenic fungi against Tuta absoluta (Lepidoptera: Gelechiidae) in tomato plants." F1000Research 13 (July 16, 2024): 800. http://dx.doi.org/10.12688/f1000research.148658.1.

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Background The tomato, Solanum lycopersicum L., is one of the most important horticultural crops that can be consumed fresh or after being processed worldwide. The tomato leaf miner (Tuta absoluta) is one of the most devastating pest to tomato plants due to its mine-feeding nature in the mesophyll tissue of the plant. Fungal entomopathogens can exist naturally in plants as an asymptote. This study aimed to detect the endophytic colonization of Beauveria bassiana and Metarhizium robertsii within tomato plants via artificial inoculation and their virulence effects on Tuta absoluta. Methods Isola

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Somodi, G. Cameron, J. B. Jones, J. W. Scott, and J. P. Jones. "Screening Tomato Seedlings for Resistance to Bacterial Spot." HortScience 29, no. 6 (1994): 680–82. http://dx.doi.org/10.21273/hortsci.29.6.680.

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A `spray-inoculation seedling screening procedure was developed for detecting resistance to Xanthomonas campestris pv. vesicatoria (Doidge) Dye, causal agent of bacterial spot of tomato (Lycopersicon esculentum Mill.). Two-week-old transplants were preconditioned under 95% humidity for 16 hours before spray inoculation and then rated for bacterial spot 2 weeks later. Resistant plants could also be distinguished from susceptible genotypes using a modified bacterial speck [Pseudomonas syringae pv. tomato (Okabe) Young, Dye, and Wilkie] screening procedure (cotyledon-dip technique). When results

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Lasik-Kurdyś, Małgorzata, Małgorzata Majcher, and Jacek Nowak. "Effects of Different Techniques of Malolactic Fermentation Induction on Diacetyl Metabolism and Biosynthesis of Selected Aromatic Esters in Cool-Climate Grape Wines." Molecules 23, no. 10 (2018): 2549. http://dx.doi.org/10.3390/molecules23102549.

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The effects of different malolactic bacteria fermentation techniques on the bioconversion of aromatic compounds in cool-climate grape wines were examined. During three wine seasons, red and white grape wines were produced using various malolactic fermentation induction techniques: Coinoculation, sequential inoculation, and spontaneous process. Volatile compounds (diacetyl and the products of its metabolism, and selected ethyl fatty acid esters) were extracted by solid phase microextraction. Compounds were identified with a multidimensional gas chromatograph—GC × GC-ToFMS with ZOEX cryogenic (N

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Feng, Yan, Peter H. Dernoeden, and Arvydas P. Grybauskas. "A Simple Pythium aphanidermatum Field Inoculation Technique for Perennial Ryegrass." HortScience 34, no. 2 (1999): 301–4. http://dx.doi.org/10.21273/hortsci.34.2.301.

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A simple field Pythium inoculation technique is needed to be able to assess cultivars for disease resistance and effectiveness of cultural practices or fungicides in the management of Pythium blight. We assessed two mixtures as inocula [i.e., an infested tall fescue (Festuca arundinacea Schreb.) seed plus wheat (Triticum aestivum L.) bran and an infested rye (Secale cereale L.) plus barley (Hordeum vulgare L.) grain mix], and three covers (black and clear plastic, and a geothermal blanket) for their ease of use and consistency in producing Pythium blight epidemics in perennial ryegrass (Lolium

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Butt, Kebin R., James Frederickson, and Richard M. Morris. "An earthworm cultivation and soil inoculation technique for land restoration." Ecological Engineering 4, no. 1 (1995): 1–9. http://dx.doi.org/10.1016/0925-8574(93)e0053-s.

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Sharma, K. K., Raghveer Singh, and Asmita Sirari. "Artificial Inoculation Technique to Produce Web Blight Disease on Mungbean." Agricultural Research Journal 60, no. 6 (2023): 853–57. http://dx.doi.org/10.5958/2395-146x.2023.00123.0.

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Sadeq, Buraq Musa, Ali Tan Kee Zuan, Susilawati Kasim, et al. "Tripartite Introductions of PGPR, Humic Acid, and N-Fertilizer Improve the Growth and Yield of Sweet Potato Under Glasshouse Conditions." Sains Malaysiana 53, no. 1 (2024): 23–37. http://dx.doi.org/10.17576/jsm-2024-5301-03.

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This study was undertaken to investigate the effects of plant growth-promoting rhizobacteria (PGPR) with humic acid (HA) as amendments on the morphological and physiological growth characteristics and yield of Sepang Oren sweet potato (Ipomoea batatas[L.] Lam). The experiment was conducted under glasshouse conditions at the Faculty of Agriculture, Universiti Putra Malaysia, for 110 days. Two factors were used in this experiment: The first factor was PGPR-HA inoculations (UPMB10, UPMRB9, and mixed strains) and non-inoculation-HA, and the second factor was the Nitrogen fertilizer levels (50, 75,

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Ali Hossain, Muhammed, Ahsan Habib, Mohammad Shafiqul Islam, Fatema Tuz - Zohura, and Md Atiqur Rahman Khokon. "Validation of Inoculation test and Screening for Resistance Sources of Mustard-Rapeseed Cultivars in Bangladesh Against Alternaria brassicicola." Current Agriculture Research Journal 6, no. 1 (2018): 08–19. http://dx.doi.org/10.12944/carj.6.1.02.

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Alternaria leaf spot or black leaf spot caused by Alternaria brassicicola is the devastating diseases of mustard-rapeseed in Bangladesh, and can cause yield loss up to 60% every year. A total of 27 cultivars of Brassica spp. where Brassica rapa (13), B. juncea (5) and B. napus (9) were selected for resistance screening against A. brassicicola. Two inoculation methods viz. detached leaf and seedling inoculation were assessed for checking the validation of inoculation technique using cultivated mustard-rapeseed varieties in Bangladesh a method to measure resistance to A. brassicicola. A signific

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Vilela, Alice. "Modulating Wine Pleasantness Throughout Wine-Yeast Co-Inoculation or Sequential Inoculation." Fermentation 6, no. 1 (2020): 22. http://dx.doi.org/10.3390/fermentation6010022.

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Wine sensory experience includes flavor, aroma, color, and (for some) even acoustic traits, which impact consumer acceptance. The quality of the wine can be negatively impacted by the presence of off-flavors and aromas, or dubious colors, or sediments present in the bottle or glass, after pouring (coloring matter that precipitates or calcium bitartrate crystals). Flavor profiles of wines are the result of a vast number of variations in vineyard and winery production, including grape selection, winemaker’s knowledge and technique, and tools used to produce wines with a specific flavor. Wine col

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Yadahalli, G. S., B. M. Doddamani, and Vidyavathi G. Yadahalli. "Pigeonpea Productivity and Soil Health as Influenced by Phosphorus Levels and AM Fungi under Different Planting Methods." International Journal of Plant & Soil Science 36, no. 5 (2024): 929–37. http://dx.doi.org/10.9734/ijpss/2024/v36i54589.

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A field experiment was conducted at ARS, Bheemarayarnagudi, Karnataka to study the effect of phosphorus graded levels and inoculation of arbuscular mycorrhizal (AM) fungi under different establishment techniques in pigeonpea during kharif season of three years (2010-13) in Upper Krishna Project command area of Karnataka. The treatment consists of phosphorus graded levels (0, 12.5, 25, 37.5 and 50kg ha-1) with inoculation VAM fungi under two establishment techniques (Transplanted and dibbled) with randomized block design. Three-year pooled data indicated that, Application of 50 kg P + VAM under

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Knight, J. D. "Delayed inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaiae." Canadian Journal of Plant Science 95, no. 2 (2015): 205–11. http://dx.doi.org/10.4141/cjps-2014-015.

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Knight, J. D. 2015. Delayed inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaiae. Can. J. Plant Sci. 95: 205–211. The persistence of perennial forage legume crops relies on the establishment of an effective symbiotic relationship with the appropriate Rhizobium species and strain. Situations can arise where a forage legume crop fails to symbiotically fix N2. This study investigates if inoculation of alfalfa with a commercial Sinorhizobium meliloti inoculant 1 yr after seeding can induce biological N2 fixation at levels similar to those achieved when the inoculant is applie

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Freeland, Emily, Whitney Cranshaw, and Ned Tisserat. "Effect of Geosmithia morbida Isolate and Temperature on Canker Development in Black Walnut." Plant Health Progress 13, no. 1 (2012): 11. http://dx.doi.org/10.1094/php-2012-0618-01-rs.

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Thousand cankers disease of black walnut (Juglans nigra) is the result of aggressive feeding by the walnut twig beetle (Pityophthorus juglandis) and extensive cankering around beetle galleries caused by the fungus Geosmithia morbida. We developed a consistent, reproducible inoculation technique to screen black walnut trees for their reaction to canker development following inoculation with G. morbida. Canker areas in one-year-old trees were not affected by the location on the stem that inoculations were made. Differences in aggressiveness of G. morbida isolates, representing different rDNA ITS

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Harp, Aulia Floribunda, Tia Setiawati, Alina Akhdiya, et al. "Application of Endophytic Bacteria using In vitro Technique to Increase Vigour of Shallots (Allium cepa L.) based on Inoculation Time." E3S Web of Conferences 483 (2024): 01002. http://dx.doi.org/10.1051/e3sconf/202448301002.

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Shallot is one of the important horticultural commodities in Indonesia. Besides having many benefits, the shallot is also the main ingredient in various traditional dishes in Indonesia. A significant increase in healthy shallot production is needed to meet the increasing demand every year. The aim of this study was to obtain the best strain of endophytic bacteria and inoculation time for increasing shallot vigour through in vitro. This study used a factorial completely randomized design with two factors, the type of bacteria and the time of inoculation which was divided into 3 stages. The firs

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Ombiri, J., V. Zinkernagel, E. M. Gathuru, O. Achwanya, and A. Lebeda. "Induction of post-infection ethylene and its role in resistance of bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum." Plant Protection Science 39, No. 3 (2011): 79–87. http://dx.doi.org/10.17221/3861-pps.

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The influence of inoculation techniques on induction of post-infection ethylene production in susceptible and resistant genotypes of bean (Phaseolus vulgaris) inoculated with the kappa race of Colletotrichum lindemuthianum was studied. Three inoculation methods (brushing, dipping and spraying) of excised leaves were compared. The brushing technique caused both the highest ethylene production at 120 h after inoculation, and better symptom development than dipping and spraying. It was, therefore, adopted to determine the post-infection ethylene production in four inoculated bean genotypes (GLP40

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Dari, Ayu Fitria Wulan, Abdur Razzaq, and Neni Noviza. "Penerapan Teknik Stress Inoculation Training (SIT) untuk Mereduksi Post Traumatic Stress Disorder (PTSD) pada Remaja Korban Kekerasaan Seksual (Studi Kasus Klien “R”)." Journal of Society Counseling 1, no. 3 (2024): 260–70. http://dx.doi.org/10.59388/josc.v1i3.277.

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This study analyzes the Application of Stress Inoculation Training (SIT) Techniques to Reduce Post Traumatic Stress Disorder (PTSD) in Adolescent Victims of Sexual Violence (Case Study of Client "R" in Betung OKUT Village). The aim of the study was to find out the symptoms of post traumatic stress disorder (PTSD) in young victims of sexual violence experienced by client "R", to find out the stress inoculation training (SIT) approach to reduce post traumatic stress disorder (PTSD) in young victims of sexual violence. Data collection techniques through observation, interviews and documentation.

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Etchebar, Christophe, Danièle Trigalet-Demery, Frédérique van Gijsegem, Jacques Vasse, and André Trigalet. "Xylem Colonization by an HrcV¯ Mutant of Ralstonia solanacearum Is a Key Factor for the Efficient Biological Control of Tomato Bacterial Wilt." Molecular Plant-Microbe Interactions® 11, no. 9 (1998): 869–77. http://dx.doi.org/10.1094/mpmi.1998.11.9.869.

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Microscopic studies of the colonization of the vascular tissues of tomato by an HrcV¯ (formerly HrpO¯) mutant strain of Ralstonia solanacearum were carried out after either root inoculation of the mutant strain alone or delayed challenge inoculation by a pathogenic strain. The use of two different marker genes, lacZ and uidA, introduced into either mutant or wild-type strains, respectively, permitted histological observation for the presence of both strains simultaneously. In roots, both strains could be found together in infected root tips and in lateral root emergence sites (lateral root cra

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Rini, Yolanda Setio, Suryati Suryati, and Lena Marianti. "Peran Konseling Individu Dengan Teknik Stress Inoculation Training (SIT) Untuk Mengatasi Stress Pada Lansia." Journal of Society Counseling 1, no. 1 (2023): 112–19. http://dx.doi.org/10.59388/josc.v1i1.104.

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This research is entitled "The Role of Individual Counseling Using Stress Inoculation Training (SIT) Techniques to Overcome Stress in the Elderly at PSLU Harapan Kita Palembang". The purpose of this stress inoculation training technique is to find out the description of stress in the elderly and the application of stress inoculation training techniques to deal with stress in the elderly. The method used in this research is a qualitative research method. The results of this study are that before carrying out individual counseling it can be seen that the elderly do not have sensitivity to the st

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Singh, R. N., and B. N. Mahto. "A Natural Inoculation-Spread Technique (NIST) for Selecting Bacterial Blight (BB)-Resistant Rice Cultivars." International Rice Research Newsletter 14, no. 3 (1989): 16–17. https://doi.org/10.5281/zenodo.7146807.

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This article 'A Natural Inoculation-Spread Technique (NIST) for Selecting Bacterial Blight (BB)-Resistant Rice Cultivars' appeared in the International Rice Research Newsletter series, created by the International Rice Research Institute (IRRI). The primary objective of this publication was to expedite communication among scientists concerned with the development of improved technology for rice and for rice based cropping systems. This publication will report what scientists are doing to increase the production of rice in as much as this crop feeds the most densely populated and land scarce na

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Gutiérrez, Walter A., and Asimina L. Mila. "A Rapid Technique for Determination of Races of Phytophthora nicotianae on Tobacco." Plant Disease 91, no. 8 (2007): 985–89. http://dx.doi.org/10.1094/pdis-91-8-0985.

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A laboratory technique for determining races of Phytophthora nicotianae on tobacco (Nicotiana tabacum) was developed and compared with a commonly used greenhouse method. The laboratory technique was based on production and inoculation of tobacco seedlings in tissue culture plates. Three P. nicotianae isolates from North Carolina previously determined as race 0 and 1 were used. Four tobacco cultivars and two breeding lines with different types of resistance were used as differential cultivars: K-326, K-346, NC-71, NC-1071, L8, and Ky14xL8. Plants were evaluated 7 and 14 days after inoculation.

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Zhao, Xinbei, Yunxia Ni, Xintao Liu, et al. "A Simple and Effective Technique for Production of Pycnidia and Pycnidiospores by Macrophomina phaseolina." Plant Disease 104, no. 4 (2020): 1183–87. http://dx.doi.org/10.1094/pdis-08-19-1795-re.

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Production of pycnidia and pycnidiospores by Macrophomina phaseolina is not often seen in vitro. The objective of this study is to develop a simple and effective technique to obtain pycnidiospores of M. phaseolina isolates in vitro and to evaluate germination rates and pathogenicity of pycnidiospores. We found M. phaseolina isolates can produce pycnidia on oatmeal agar (OMA) under ultraviolet light with 365 nm wavelength (UV). For evaluating the effect of OMA and UV on growth of M. phaseolina, combinations of two agar media and three lighting conditions were tested. The results confirm that al

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Atmaca, Emel. "Comparison of the Effects of Arbuscular Mycorrhizal Fungal (AMF) Spore Inoculation on The Growth of Mexican Marigold (Tagetes erecta L.) Plants Grown with Two Different Techniques." International Journal of Engineering and Science Invention 13, no. 10 (2024): 01–08. http://dx.doi.org/10.35629/6734-13100108.

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In recent years, the importance and utilization of plant-root-fungal mutualistic symbiosis in crop production have been increasing. In this system, arbuscular mycorrhizal fungi (AMF) spores are mostly used as the production material. The abundant production of healthy spores is an influential factor in the widespread use of this microbial fertilizer. Various cultivation and inoculation techniques have been developed for AMF spore production. Soil and substrate-based production techniques are widely used for the large-scale production of AMF spores. However, beyond the commercial scale, it is i

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