Dissertations / Theses on the topic 'Insects Classification'

Note: page 74 is missing from archival and circulating copies
The present state af knowledge of the order Thysanaptera in Canada is reviewed. The aoder has been sorely neglected since Provenchar submitted a list af 35 eastern Canadian species in 1890. In contrast to the latest previaus estimate of 102 species (Heming, 1979), the number of species found in this study is 174 (143 described and 31 undescribed species). One new genus and five new species are reported as well as the European species Odontothrips biuncus which has been found for the first time in North America. Descriptions, illustrations and a dichatamaus key to the Canadian genera of the Thysanaptera ara given for the first time. Also included are lists of the species. hitherto found in Canada along with their localities•• and habitats•• In addition, an alternative computer-compatible key is presented for the suborder Tubullfera. The distribution and study af the order in Canada are discussed and notes on collection, classification, biology and economic importance are given.
Ce travail revise l’etat de nos cannais.ance. sur l’ordre de. Thysanopteres au Canada. Ce groupe a fait l’objet de peu d’etudes depuis la publication de Provencher (1890), qui enumerait 35 especes pour l’est du Canada. Dans la presente etude, nous rapportons . 174 especes (143 especes decrites, 31 especes non decrit••) alors qu’Heming (1979) n’en citait que 102 especes. pour ce pays. On y cree un nouveau genre et 5 nouvelles especes, en plus de recolter pour la premiere fois l’espece europeanne, Odonthrips biuncus en Amerique du Nord. L’auteur decrit et illustre pour la premiere fois. les. genres de Thvsanoptere. representes dans la fauna canadienne. De plus, on y trouve une liste des especes presentes au Canada, accompagnee de notes sur leur repartition geographique et leur habitat. On y inclut un tableau dichotomique conventionnel ainsi qu’un tableau de determination adape a l’ordinateur. pour Ie sous-ordre des Tubuliferes. Ce travail fournit egalemant, pour les Thysanopteres du Canada, une discussion sur leur repartition Geographique et des renseignements sur leur recolte et classification, leur biologie et importance economique.

Orientador: Francisco de Assis Ganeo de Mello
Banca: Francisca Carolina do Val
Banca: Sônia A. Casari
Resumo: O presente trabalho teve por objetivo estudar, do ponto de vista taxonômico, as espécies de Lutosa Walker, 1869 (Orthoptera, Ensifera, Stenopelmatoidea, Anostostomatidae) que ocorrem nas florestas do leste do Estado de São Paulo, Brasil, abrangendo análises da morfologia corporal externa, do complexo fálico e espermateca. Além da re-descrição do gênero e de três espécies já conhecidas, treze novas foram descritas e uma chave dicotômica de identificação é proposta para todas as espécies examinadas. Com base na estrutura de certos elementos que compõem o complexo fálico, dois grupos de espécies foram reconhecidos e definidos. Ainda no que concerne à morfologia da genitália masculina, uma proposta de terminologia para as diferentes áreas do apódema epifálico é apresentada. Dados preliminares sobre hábitos, ecologia e comportamento de espécies de Lutosa são fornecidos.
Abstract: The aim of the present work was to study, from a taxonomic perspective, the species of Lutosa Walker, 1869 (Orthoptera, Ensifera, Stenopelmatoidea, Anostostomatidae) from the eastern forests from the State of São Paulo, Brazil, including analyses of external body morphology, phallic complex and spermatheca. Besides re-describing the genus and three of its valid species, thirteen new ones were described, and a dichotomic identification key for all examined species is presented. Based on the structure of certain elements that comprise the phallic complex, two species groups were recognized and defined. Still regarding male genital morphology, a terminological proposal for the different areas of the epiphallic apodeme is presented. Preliminary data on habits, ecology, and behavior of Lutosa species are given.
Mestre

Um dos grandes desafios em mineração de dados é a integração de dados temporais ao seu processo. Existe um grande número de aplicações emergentes que envolvem dados temporais, incluindo a identificação de transações fraudulentas em cartões de crédito e ligações telefônicas, a detecção de intrusão em sistemas computacionais, a predição de estruturas secundárias de proteínas, a análise de dados provenientes de sensores, entre muitas outras. Neste trabalho, tem-se interesse na classificação de séries temporais que representam sinais de áudio. Como aplicação principal, tem-se interesse em classificar sinais de insetos coletados por um sensor óptico, que deve ser capaz de contar e classificar os insetos de maneira automática. Apesar de serem coletados opticamente, os sinais capturados se assemelham a sinais de áudio. O objetivo desta pesquisa é comparar métodos de classificação por similaridade e por extração de atributos que possam ser utilizados no contexto da classificação de insetos. Para isso, foram empregados os principais métodos de classificação de sinais de áudio, que têm sido propostos para problemas como reconhecimento de instrumentos musicais, fala e espécies animais. Neste trabalho, é mostrado que, de modo geral, a abordagem por extração de atributos é mais eficaz do que a classificação por similaridade. Mais especificamente, os melhores resultados são obtidos com a utilização de coeficientes mel-cepstrais. Este trabalho apresenta contribuições significativas em outras aplicações, também relacionadas à análise de séries temporais e sinais de áudio, por similaridade e por extração de atributos
One of the major challenges in data mining is the integration of temporal data to its process. There are a number of emerging applications that involve temporal data, including fraud detection in credit card transactions and phone calls, intrusion detection in computer systems, the prediction of secondary structures of proteins, the analysis of data from sensors, and many others. In this work, our main interest is the classification of time series that represent audio signals. Our main interest is an application for classifying signals of insects collected from an optical sensor, which should count and classify insects automatically. Although these signals are optically collected, they resemble audio signals. The objective of this research is to compare classification methods based on similarity and feature extraction in the context of insects classification. For this purpose, we used the main classification methods for audio signals, which have been proposed for problems such as musical instrument, speech and animal species recognition. This work shows that, in general, the approach based on feature extraction is more accurate than the classification by similarity. More specifically, the best results are obtained with mel-frequency cepstrum coefficients. This work also presents significant contributions in other applications, also related to the analysis of time series and audio signals by similarity and feature extraction

As a result of research carried out within the last decade to assess the diversity of macroinvertebrates of the Salt River in the Western Cape Province, South Africa, surveys of macroinvertebrates of 20 sites on 11 selected rivers from the same mountain range source were undertaken. This was done to make a preliminary assessment of the conservation status of the rivers of this region. Aquatic insects from the orders Ephemeroptera, Odonata, Plecoptera, Megaloptera, Trichoptera and the dipteran family Simuliidae were collected using techniques to maximize the number of taxa found. The insects collected were identified to species level where possible. Water physicochemical parameters were recorded at all sites for each sampling trip to characterize these rivers and to establish a set of baseline data for future comparisons. These parameters included measurements made on site and analysis of the concentrations of all the major ions in water samples in the laboratory. Multivariate analyses including Principle Components Analysis and Canonical Correspondence Analysis were used to reveal patterns within the water physicochemistry and species distributional data. Results include the identification of 123 species from 70 genera and 30 families. A total of 31 species were found to be undescribed, of which 17 were not previously collected and are thus completely new to science. In addition, four of these species could not be placed into any known genus. The analysis of water physicochemistry showed a clear distinction between rivers of Table Mountain sandstone and Bokkeveld shale origin. Downstream effects of anthropogenic influences were discernable too. Distinct macroinvertebrate assemblages were found based upon stream order and water chemistry composition. pH proved to be the most important driver of invertebrate assemblage composition. The high levels of endemism of the macroinvertebrates found within the upper reaches of these rivers and their degree of ecological specialization make these systems a priority for the conservation of aquatic biodiversity on a national and global scale.

Besides the unsustainable exploitation, farming activities and economic development activities, forest fire is considered as one of the major threats to biodiversity and deforestation in Vietnam. In forest ecosystems, any changes in insect communities can affect species composition, nutrient cycling and numerous other ecological processes. The impact of forest fires, however, is not equal to different insect groups. In this paper, we study the impact of forest fires to hymenopteran insects at Copia species-use forest, Son La Province. This is the first time this kind of study has been taken place in Vietnam
Bên cạnh việc khai thác không bền vững, tập quán canh tác và các hoạt động phát triển kinh tế, cháy rừng được xem là một trong những mối đe dọa chính ảnh hưởng tiêu cực tới đa dạng sinh học và mất rừng ở Việt Nam. Trong các hệ sinh thái rừng, những thay đổi trong cấu trúc của quần xã côn trùng có ảnh hưởng tới thành phần loài, chu trình dinh dưỡng và rất nhiều quá trình sinh thái khác. Tuy nhiên mức độ ảnh hưởng của cháy rừng tới các nhóm côn trùng khác nhau là khác nhau. Trong bài báo này, chúng tôi nghiên cứu tác động của cháy rừng tới nhóm côn trùng cánh màng tại khu rừng đặc dụng Copia, tỉnh Sơn La. Đây cũng là lần đầu tiên tác động của cháy rừng tới nhóm côn trùng cánh màng được thực hiện ở Việt Nam

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Insects cause significant losses of stored grains in both quantity and quality. In the scenary, it is of paramount importance an early identification of insects in grains to take control measures. Instead of sampling and visual/laboratory analysis of grains, we propose to carry out the insects identification task automatically, using computational methods to perform endoscopic video analysis. The videos are recorded inside of grains warehouses by an endoscopic camera. As the classification process of moving objects in video rely heavily on precise segmentation of moving objets, we propose a new method of background subtraction and compared their results with the main methods of the literature according to a recent review. The main innovation of the background subtractionmethod rely on the binarization process that uses two thresholds: a global and a local threshold. The binarized results are combined by adding details of the object obtained by the local threshold in the result of the global threshold. Experimental results performed through visual analysis of the segmentation results and using a SVM classifier, suggest that the proposed segmentation method produces more accurate results than the state-of-art background subtraction methods.
Insetos causam perdas quantitativas e qualitativas significantesemgrãos armazenados. Neste cenário, é de vital importância uma identificação rápida de insetos em grãos para que sejam tomadas medidas de controle. Ao invés de coletar amostras de grãos para análise visual/laboratorial, é proposta a realização desta tarefa de identificação de formaautomática, usando métodos computacionais para a análise de vídeos endoscópicos. Os vídeos são gravados dentro de armazéns de grãos usando câmera endoscópica. Como o processo de classificação de objetos em movimento em vídeo depende fundamentalmente de uma segmentação de objeto precisa, é proposto um novo método de segmentação por subtração de plano de fundo e comparado seus resultados com os principais métodos da literatura de acordo com um estudo de revisão recente. A principal inovação neste método de subtração de plano de fundo está no processo de binarização que usa dois thresholds: um global e um local. Os resultados binarizados são combinados pela adição de detalhes do objeto obtido pelo threshold local no resultado do threshold global. Resultados experimentais, realizados através de análise visual dos resultados de segmentação e usandoumclassificadorSVMindicamque o método de segmentação proposto produz melhores resultados que métodos do estado da arte atual da literatura de subtração de plano de fundo.

Experten dreier deutscher Hochschulen entwickelten gemeinsam mit der Ostdeutschen Gesellschaft für Forstplanung mbH ein neues, forstliches Monitoringsystem. Das Verbundprojekt „Entwicklung eines forstlichen Monitoringsystems unter Berücksichtigung von Kohlenstoffspeicherung und Klimaanpassung“ (FOMOSY-KK) wird vorgestellt.

Many insects are considered as potential sources of food for humans because of their high content of protein, fat, minerals and vitamins. Tessaratoma papillosa Drury, a stink bug of longan tree has long been consumed by many people in Vietnam as a supplemental source of nutrition. This insect is one of the most severe pests of longan tree. The use of this insect as supplementary nutrient could actively reduce the pests on longans, contributing to increase productivity and quality of longan fruit. The aim of this paper is to estimate the volume of mature and young Tessaratoma papillosa on longan trees in Son La Province, Vietnam.
Nhiều loài côn trùng được coi là nguồn thực phẩm tiềm năng của con người vì chúng có chứa hàm lượng cao protein, chất béo, chất khoáng và vitamin. Loài bọ xít nhãn Tessaratoma papillosa Drury, từ lâu đã được nhiều người dân ở Việt Nam sử dụng như là một nguồn thực phẩm bổ sung dinh dưỡng. Loài bọ xít nhãn là một trong những loài gây hại nghiêm trọng nhất đối với cây nhãn. Việc sử dụng loài côn trùng này không những bổ sung chất dinh dưỡng cho con người mà còn góp phần chủ động giảm loài sâu hại trên nhãn, góp phần tăng năng suất và chất lượng trái cây nhãn. Mục đích của bài viết này là để ước tính khối lượng trưởng thành và ấu trùng loài Tessaratoma papillosa trên cây nhãn ở tỉnh Sơn La, Việt Nam.

Les espèces et genres connus d'Aeschnidiidae sont révisés, des espèces nouvelles et des genres nouveaux européens du jurassique supérieur et du crétacé inférieur sont décrits, augmentant ainsi de façon significative le nombre de taxons de cette famille. Les données morphologiques connues des adultes et des larves sont discutées, de nouveaux caractères de la larve, de la nervation alaire et corporelle de l'adulte sont exposés. Dans un premier temps, les révisions systématiques et des analyses phylogénétiques de plusieurs groupes-frères potentiels des (Anisoptera + Aeschnidiidae) - à savoir les Tarsophlebiidae, les Isophlebioidea, les 'Heterophlebioptera' et les Stenophlebioptera - sont tentées. Ensuite une analyse phylogénétique visant à clarifier les relations de parenté entre Aeschnidiidae et Anisoptera est proposée. Il en résulte, notamment grâce aux caractères larvaires, que les Aeschnidiidae sont des Anisoptera : Cavilabiata. Enfin, une phylogénie interne des Aeschnidiidae est proposée. Les résultats demandent confirmation, mais il ressort que la taille de ces insectes va décroissante. Aux très grandes espèces du jurassique supérieur succèdent les très petites formes du crétacé inférieur. Enfin, les causes de la disparition de ce groupe mésozoi͏̈que d'Odonata très spécialisé sont discutées, prédation aviaire versus bouleversement des écosystèmes aquatiques apparus au cénomanien

Au sein des Pentatomomorpha (Insectes, Hétéroptères), les Aradidae forment un groupe de punaises mycétophages présentant plusieurs degrés de réduction alaire. Ce groupe est cependant peu étudié à l’heure actuelle. Le but de ce travail est d’une part d’apporter des informations sur l’habitat de ces espèces forestières inféodées au bois mort, et d’autre part d’établir une première phylogénie cladistique du groupe. Les relations entre des caractéristiques écologiques de l'habitat des espèces et les hypothèses d'évolution, comme le développement des ailes, sera abordé. Dans un premier temps, l’écologie du groupe a été étudiée en forêt de Fontainebleau et de Rambouillet (France). Les caractéristiques des pièces de bois où se trouvent et se reproduisent les espèces les plus courantes ont été étudiées, et leur abondance a été mise en relation avec des variables à plus grande échelle tels que le volume et la surface de bois mort sur les placettes d’étude. Les résultats montrent l’importance de la disponibilité de bois mort frais de chêne et de charme pour les espèces Aneurus avenius, tandis qu’Aneurus laevis présente un spectre d’hôte plus large en termes de décomposition du bois. Pour les espèces du genre Aradus, les données sont moins nombreuses mais l’habitat semble sensiblement différent, avec une plus grande influence des arbres sénescents de gros diamètre et des espèces plus ou moins opportunistes. Dans un deuxième temps, une phylogénie du groupe a été construite sur la base de caractères morphologiques et moléculaires. Les Aradidae forment un groupe monophylétique, et six des huit sous-familles d’Aradidae sont monophylétiques. Certains résultats obtenus différent sensiblement de ce qu’avaient proposé de précédents auteurs, notamment en ce qui concerne la position des Calisiinae au sein de la famille. Le replacement du développement alaire sur la phylogénie montre que les ailes ont été perdues chez différentes sous-familles de façon indépendante. Si le jeu de données actuel ne permet pas de conclure définitivement sur les évènements évolutifs survenus au sein d’une même sous-famille, la possible ré-évolution des ailes à partir d’un ancêtre aptère n’est pas exclue. De même, il est encore difficile d'évaluer le lien entre les caractéristiques de l'habitat des Aradidae et leur patron d'évolution
Among Pentatomomorpha (Insecta Heteroptera), Aradidae are a group of mycetophagous bugs exhibiting different degree of wing reduction. This group is though poorly studied. The aim of this work is in one hand to give new information on habitat for some forest dwelling species, dependant on dead wood, and in the other hand to establish a first cladistic phylogeny of the family. Relations between ecological characteristics of the habitat of species, and hypotheses of evolution, as the development of wings, will be introduced. First, the ecology was studied in two French forests, Fontainebleau and Rambouillet. The characteristics of pieces of wood where most common species are present and reproduce were studied, and their abundance was linked with stand level variables at a larger scale, like dead wood volumes and surfaces on study plots. The results show that the availability of fresh dead wood of oak is important for Aneurus avenius, and that Aneurus laevis has a larger host range relating to decay degree. For species belonging to the genus Aradus, few data are available but the habitat seems quite different, with a stronger effect of large diameter senescent trees, and species more or less opportunists. Second a phylogeny of the family was built, based on morphological and molecular data. Aradidae are a monophyletic group, and six of the eight known subfamilies are monophyletic. Some results are quite different from the results of previous authors, particularly concerning the position of the subfamily Calisiinae within the family. Finally, repositioning of wings development on the phylogeny shows that wings were lost independently in different subfamilies. If the current dataset does not allow definitive findings of evolutionary events that occurred within each subfamily, the possible re-evolution of wings from a wingless ancestor is not excluded. Similarly, it is still difficult to assess the relationships between habitat characteristics of Aradidae and their evolution pattern

Les premières 'crises' concernant les insectes ailés, du Carbonifère supérieur au Trias moyen, sont étudiées d'après le clade super-ordinal Archaeorthoptera. Nous proposons des homologies de nervation alaire, une taxonomie révisée et une hypothèse de phylogénie. La diversité taxonomique est étudiée à partir d'un décompte des espèces connues. La disparité morphologique est étudiée d'après les types de vol et les appareils de stridulation. Au passage Carbonifère - Permien, la diversité taxonomique spécifique relative des Orthoptera passe de 5 à 70%, ce qui semble lié aux modifications des biotopes contemporains. Le passage Permien - Trias est marqué par l'extinction de l'ordre Caloneurodea, ayant une forte disparité morphologique. Au Trias, les groupes possédant un appareil de stridulation (Ensifera et Titanoptera) ont une forte diversité taxonomique. L'évolution des Archaeorthoptera a été marquée par des modifications des biotopes et par des innovations apparues au sein de ce groupe
The first known crises that concerned the winged insects, between the Late Carboniferous and the Middle Triassic, are studied after the supra-ordinal clade Archaeorthoptera. We propose homologies of the wing venation, a revised taxonomy, and a phylogenetic hypothesis. The taxonomic diversity is studied after the count of the known species. The morphological disparity is related to the flight modes and the stridulatory apparatuses. At the Carboniferous - Permian boundary, the specific relative taxonomic diversity changes from 5 to 70%, which seems to be related to contemporaneous changes in biota. The Permian - Triassic boundary is marked by the extinction of the order Caloneurodea, which has a high morphological disparity. The Triassic insects possessing a stridulatory apparatus (Ensifera and Titanoptera) have a high taxonomic diversity. The evolution of the Archaeorthoptera is marked by modifications of biotopes and by several innovations acquired within the group

Sandwichplatten sind effiziente Leichtbauelemente. Sie werden im Fahrzeugbau, in der Luft- und Raumfahrt, im Werkzeugmaschinenbau und überall dort, wo es auf geringe bewegte Massen ankommt, eingesetzt. Sandwichplatten sind oft Halbzeuge. Sandwichbauteile haben oft einen aufwendigen Herstellungsprozess, der eine Wärmebehandlung, die höhere Genauigkeiten ausschließt, beinhaltet. Aus diesen Gründen ist es oft sinnvoll, Inserts erst im Nachhinein in die fertige Sandwichplatte bzw. in das fertige Sandwichbauteil einzusetzen. Diese Inserts haben unterschiedliche Aufgaben. Sie dienen z.B. der Aufnahme von Schrauben, Bolzen und Lagern. Die vorgestellte Verbindung entspricht durch ihre spezielle Geometrie den hohen Anforderungen für Verbindungselemente in den genannten Gebieten hinsichtlich Genauigkeit und Stabilität. Es wird der Konstruktionsprozess eines Inserts für Sandwichplatten aus CFK- Verbundmaterial beschrieben. Ausgehend von einer patentierten Lösung der Autoren findet eine schrittweise Produktverbesserung bis hin zu einem einsatzfähigen Prototypen statt. Durch die Insertverbindung können für den Maschinenbau erforderliche Genauigkeiten nun mit CFK- Halbzeugen umgesetzt werden. Die Anwendung dieses Leichtbaupotentials führt zur Energieeinsparung und zum Transfer von CFK- Anwendungen in den Maschinenbau.

Up to date, three species of the genus Flavopimpla have been known from Vietnam, of which two species were described as new on the basis of the material collected from the country, viz. F. lanugo Pham, Broad & Wägele, 2013 from Xuan Son NP, Phu Tho Province and F. vinhcuuensis Pham, Broad & Wägele, 2013 from Phu Ly, Dong Nai Province. Resulted from recent field survey, one species Flavopimpla nigromaculata (Cameron) is recorded for the first time from the country and included to the previous key to already known Flavopimpla species of Vietnam. In addition, the distribution range of F. lanugo is extended northwards.
Cho đến nay, 3 loài thuộc giống ong cự Flavopimpla đã được ghi nhận ở Việt Nam, trong đó hai loài được miêu tả gần đây dựa trên các mẫu vật thu được trong nước bao gồm F. lanugo Pham, Broad & Wägele, 2013 ở Vườn Quốc gia Xuân Sơn, tỉnh Phú Thọ và F. vinhcuuensis Pham, Broad & Wägele, 2013 ở Phủ Lý, tỉnh Đồng Nai. Dựa trên các chuyến khảo sát gần đây, loài Flavopimpla nigromaculata (Cameron) được ghi nhận lần đầu tiên cho khu hệ côn trùng Việt Nam và được đưa vào khóa định loại tới loài của giống này đã có trước đây. Bên cạnh đó, vùng phân bố của loài F. lanugo cũng được ghi nhận mở rộng về phía bắc.

The diversity, classification and historical biogeography of the mayfly family Prosopistomatidae are explored. First, the higher classification of the Ephemeroptera is reviewed, focussing on the phylogenetic placement of the Prosopistomatidae relative to other mayfly families. All relevant literature from 1762 to 2010 is synthesized. Baetiscidae are established as the probable sister lineage of Prosopistomatidae, the two constituting the superfamily Baetiscoidea. Next, qualitative morphological variation within the Prosopistomatidae is reviewed and revised, emphasizing nymphs because imaginal specimens are few. The labium and associated structures and the hypopharynx of nymphs, and the highly-derived wing venation of the imaginal stages, are re-interpreted. The structure of the male tarsal claws changes considerably between subimago and imago, which, together with deeply scalloped ridges on male imaginal forelegs and unusual pits on the female thorax, are interpreted as providing an unusual mating mechanism. These structures provide morphological characters for species definition and phylogenetic analyses. Two approaches to species delimitation are explored. First, morphometric variation is analysed using Principal Component Analysis, revealing groupings that can be interpreted as species, although there is some overlap between them. Discriminant Function Analysis shows that head width and carapace shape have the most value in identifying nymphs of different species. The carapace of Prosopistoma nymphs is shown to grow allometrically and gradually, in contrast with that of Baetisca, indicating a difference in early ontogeny. Second, an Artificial Neural Network algorithm applied to nymphal morphological characters accurately identified species. This computer-driven artificial intelligence method has power to provide future easy-to-use electronic identification aids. Phylogenetic analysis of nymphal morphology using the parsimony method shows two clades of Prosopistomatidae, one sharing characters with the type species, Prosopistoma variegatum and the other predominating in Africa, although also occurring in Asia; these clades are named the “P. variegatum” and “African” clades, respectively. Parsimony analysis of adult morphology supports these two clades, but supertree analysis obscures the relationships, nesting the “P. variegatum” lineage within the other clade. Preliminary molecular phylogenetic analysis of the 16S rRNA, (mitochondrial) 18S rRNA and Histone-3 genes using Bayesian Inference methods does not support the two clades shown by morphology. Instead, there is a strong relationship between the European species and one African species, with the single Asian representative being most distantly related. These results are limited by lack of fresh material, patchy taxon sampling, and problems with finding suitable primers. A molecular clock program, BEAST, calibrated using fossils, suggests divergence times for the oldest crown-group Prosopistoma clade, represented by the Asian P. wouterae, of about 131 Ma, with the youngest species, the African P. crassi, of 1.21 Ma. Stem-group relationships are analysed using parsimony analysis, focussing on wing characters of the Baetiscoidea, other extant mayfly lineages, and extinct stem-group lineages. This suggests that the Baetiscoidea diverged from main-line Ephemeroptera earlier than any other extant mayfly lineage. This approach expands upon ideas hinted at by earlier scientists. Finally, historical biogeographical analysis of the distribution of known Baetiscoidea s.s. stem-group fossils implies a once Pangean distribution of the lineage. Changing palaeo-climate, catastrophic extinction events and plate tectonic movements in relation to the distribution of crown-group species are reviewed. Other approaches to historical biogeography that build on both morphological and molecular phylogenies are used to interpret disperalist and vacarianist arguments. Distribution patterns of eight unrelated freshwater organisms which share a similar distribution pattern are compared, assuming that shared patterns indicate similar historic biogeographic processes. The distribution of recent Prosopistoma species is seen to be the product of evolution resulting from both vicariance and dispersal. In conclusion, this thesis encompasses a variety of disciplines. It successfully recognises new characters and distinguishes previously unknown species. It uses new approaches to delimiting species and known methods to determine phylogeny from several angles. The analysis of stem-group relationships offers an insight into possible early lineage splitting within Ephemeroptera. Interpretation of historical biogeography allows for both a Gondwanan origin of Prosopistomatidae, with rafting of species on the Deccan plate to Asia, and for subsequent dispersal from Asia down to Australia and across to Europe, and possibly back to Africa.

1. Avaliar diferenças nas comunidades de insetos aquáticos de quatro bacias hidrográficas do estado de São Paulo com diferentes graus de ocupação, relacionando-as em função dos usos da terra e declividade do terreno na sub-bacia a montante dos locais de coleta e em uma zona tampão de 50m, assim como em função de variáveis químicas e físicas da água. 2. Avaliar alterações nas comunidades de insetos aquáticos provenientes de locais com presença ou ausência de mata ripária nas margens. Os dados referem-se às coletas de insetos aquáticos com cestos com substrato artificial; estes ficaram 44 dias em exposição, entre agosto e outubro de 2002. As bacias estudadas foram Alto Paranapanema, Peixe, Aguapeí e São José dos Dourados. Para o primeiro estudo, seis amostras de cada rio foram tomadas, cujas faunas foram identificadas e contadas; Chironomidae foram identificados em nível genérico, os demais insetos, em família. Para interpretação dos resultados, utilizou-se números absoluto e percentual de indivíduos e táxons; índices de diversidade, riqueza, uniformidade e quantidade de táxons de Ephemeroptera, Plecoptera e Trichoptera. Para o geoprocessamento, a partir de mapas digitais, delimitou-se a sub-bacia a montante dos locais de coleta e a zona tampão de 50m ao redor dos rios, para cálculo da percentagem dos usos da terra e da declividade. Para as análises estatísticas, utilizou-se o índice de correlação de Pearson, análise de variância e análise de correspondência. Para o segundo estudo, amostras provenientes dos rios Aguapeí e São José dos Dourados foram tomadas. A fauna foi identificada nos mesmos níveis taxonômicos que o outro estudo. Como resultado do primeiro estudo, trinta e uma famílias foram identificadas, com dominância de Elmidae, Leptohyphidae, Leptophlebiidae, Chironomidae, Simuliidae e Hydropsychidae. Dente as variáveis da água testadas, dez foram capazes de caracterizar os rios. Os usos da terra também foram característicos de alguns rios. Estudo 2. Neste estudo foi encontrado menor número de indivíduos e de táxons nas amostras de mata, entretanto, as análises estatísticas demonstraram que a maior riqueza está relacionada aos locais de mata. Os resultados destes dois estudos permitiram inferir que a preservação da mata ripária imediatamente adjacente ao local é que influencia na comunidade de insetos aquáticos. Os elevados números de indivíduos e de táxons encontrados nos rios das bacias de maior grau degradação pode estar relacionado ao fato controverso de haver um corredor de mata ripário mais bem preservado ao longo destes rios que naqueles de menor ocupação antrópica. Até o presente estudo, pouco era conhecido sobre a entomofauna aquática dos rios aqui estudados; novos táxons foram encontrados, aumentando o conhecimento sobre a diversidade no estado. Outros estudos são necessários para verificar sobre a influência local da mata ripária sobre a comunidade de insetos aquáticos.
1. To evaluate differences in the aquatic insects communities of four watersheds from the State of São Paulo showing different degrees of human occupation, relating them to the landuses and terrain slope of the watershed and the 50m’s buffer zone upstream of the sample sites as well as in relation to the chemical and physical water variables. 2. To evaluate alterations in the aquatic insects communities living in places showing presence/absence of riparian forest. The data were based on samples of aquatic insects collected with artificial substrates baskets; the exposure time was 44 days between August and October/2002. The watersheds studied were Alto Paranapanema, Peixe, Aguapeí and São José dos Dourados. For the first study, six samples were taken from each river and the fauna was identified and counted, Chironomidae were identified until genus level while the other insects, until family. To analyze the results, total and percentage numbers of individuals and taxons were used, as well as some community indexes, diversity, richness, evenness and total number of Ephemeroptera, Plecoptera and Trichoptera. For the remote sensing methods, information about the watersheds and the 50m’s buffer zone upstream the sample sites were generated from digital maps. The landuses and terrain slope of those areas were calculated. Pearson’s correlation index, analysis of variance and analysis of correspondence were used for the statistical analyses. For the second study, samples from Aguapeí and São José dos Dourados rivers were taken. The faunas were identified in the same levels following the other study. As result of the first study, thirty-one families were identified. Elmidae, Leptohyphidae, Leptophlebiidae, Chironomidae, Simuliidae and Hydropsychidae showed numeric dominance. Ten out 19 chemical and physical variables tested were able to characterize the rivers. The land uses were characteristic of some rivers as well. Study 2. In that study, the samples from forest showed fewer numbers of individuals and taxons, although the statistical analysis showed that, in fact, the greatest richness was associated to places where the forest was present. Both studies permitted us to make the inference that the forest immediately adjacent to the sample site is the most important factor for that fauna. The highest numbers of individuals and taxons from rivers with greatest impacted watersheds may be related to the presence of a better preserved riparian forest surrounding the rivers. Until the present study, little was known about the entomological aquatic fauna of those rivers; new taxons were found increasing the knowledge about the diversity of the State. Other studies are necessary to confirm about the local influence of riparian forest on the insect’s aquatic community.

A partir de 148 amostras com parasitóides da família Braconidae, provenientes de levantamentos realizados com as moscas-das-frutas no Estado de São Paulo, foi feito estudo taxonômico, de distribuição e de associação com as moscas hospedeiras e fruteiras. Em 33 municípios, foram coletados 3.009 exemplares. A subfamília Opiinae foi a mais abundante com 96,2% dos exemplares. Apenas 3,8% dos parasitóides pertenciam à subfamília Alysiinae. Foram coletadas seis espécies de braconídeos: Doryctobracon areolatus (Szépligeti), Doryctobracon brasiliensis (Szépligeti), Utetes anastrephae (Viereck), Opius bellus (Wesmael) e Opius sp. (Wesmael), além do alisiíneo Asobara anastrephae (Muesebeck). A maior parte dos braconídeos (77,5%) pertencia a D. areolatus. Esta espécie foi associada ao maior número de espécies frutíferas (26), em 7 famílias, e ocorreu na maioria dos municípios amostrados (30). Foi associada a Anastrepha fraterculus (Wied.), A. obliqua (Macquart) e A. amita Zucchi, sendo obtida pela primeira vez de larvas de moscas em frutos de wampi, Clausena lansium (Lour) Skeels, e de canela-batalha, Cryptocarya aschersoniana Mez. Doryctobracon brasiliensis foi associada apenas A. fraterculus, sendo registrado pela primeira vez em ameixa-japonesa, Prunus salicina Lindl. Não foi possível associar as demais espécies de parasitóides às moscas-das-frutas. Opius sp. foi associada às larvas em canela-batalha (primeiro registro de planta associada). A percentagem de parasitismo de tefritídeos nos 33 municípios foi de 7,75%, variando de 0,02% a 40%. Foi elaborada uma chave de identificação para as espécies.
This work presents the results of a survey of braconid fruit fly parasitoids from 33 localities in the State of São Paulo. In addition to the taxonomic studies, data of geographical distribution and association of braconid species to insect hosts and associated plants were also performed. A total of 3,009 specimens were colleted. The subfamily Opiinae was the most abundant with 96.2% of specimens colleted, and only 3.8% of the parasitoids belonged to the subfamily Alysiinae. Six species of braconids were collected: Doryctobracon areolatus (Szépligeti), Doryctobracon brasiliensis (Szépligeti), Utetes anastrephae (Viereck), Opius bellus (Wesmael) and Opius sp. (Wesmael), beyond the alisiíneo Asobara anastrephae (Muesebeck). Most of the braconids (77.5%) belonged to D. areolatus. This species was associated with the highest number of fruit tree species (26), in 7 families, and occurred in the majority of the cities sampled (30). It was associated to Anastrepha fraterculus (Wied.), A. obliqua (Macquart) and A. amita Zucchi, and it is recorded for the first time from fruit fly larvae in wampi, Clausena lansium (Lour) Skeels, and in "canela-batalha" fruits, Cryptocarya aschersoniana Mez. Doryctobracon brasiliensis was associated only to A. fraterculus, and it is recorded for the first time in fruit fly larvae in plum, Prunus salicina Lindl. It was not possible to associate the other parasitoid species to the fruit fly species. Opius sp. was associated with the fruit fly larvae in "canela-batalha" (first record of associate plant). The percentage of tephritid parasitism in the 33 cities was of 7.75%, varying from 0.02% to 40%. A key to identification for the braconid species was elaborated.

Diversas aplicações são responsáveis por gerar dados ao longo do tempo de maneira contínua, ordenada e ininterrupta em um ambiente dinâmico, denominados fluxo de dados. Entre possíveis tarefas que podem ser realizadas com estes dados, classificação é uma das mais proeminentes. Devido à natureza não estacionária do ambiente responsável por gerar os dados, as características que descrevem os conceitos das classes do problema de classificação podem se alterar ao longo do tempo. Por isso, classificadores de fluxo de dados requerem constantes atualizações em seus modelos para que a taxa de acerto se mantenha estável ao longo do tempo. Na etapa de atualização a maior parte das abordagens considera que, após a predição de cada exemplo, o seu rótulo correto é imediatamente disponibilizado sem qualquer atraso de tempo (latência nula). Devido aos altos custos do processo de rotulação, os rótulos corretos nem sempre podem ser obtidos para a maior parte dos dados ou são obtidos após um considerável atraso de tempo. No caso mais desafiador, encontram-se as aplicações em que após a etapa de classificação dos exemplos, os seus respectivos rótulos corretos nunca sã disponibilizados para o algoritmo, caso chamado de latência extrema. Neste cenário, não é possível o uso de abordagens tradicionais, sendo necessário o desenvolvimento de novos métodos que sejam capazes de manter um modelo de classificação atualizado mesmo na ausência de dados rotulados. Nesta tese, além de discutir o problema de latência na tarefa de classificação de fluxo de dados não estacionários, negligenciado por boa parte da literatura, também sã propostos dois algoritmos denominados SCARGC e MClassification para o cenário de latência extrema. Ambas as propostas se baseiam no uso de técnicas de agrupamento para a adaptação à mudanças de maneira não supervisionada. Os algoritmos propostos são intuitivos, simples e apresentam resultados superiores ou equivalentes a outros algoritmos da literatura em avaliações com dados sintéticos e reais, tanto em termos de acurácia de classificação como em tempo computacional. Aléem de buscar o avanço no estado-da-arte na área de aprendizado em fluxo de dados, este trabalho também apresenta contribuições para uma importante aplicação tecnológica com impacto social e na saúde pública. Especificamente, explorou-se um sensor óptico para a identificação automática de espécies de insetos a partir da análise de informações provenientes do batimento de asas dos insetos. Para a descrição dos dados, foi verificado que os coeficientes Mel-cepstrais apresentaram os melhores resultados entre as diferentes técnicas de processamento digital de sinais avaliadas. Este sensor é um exemplo concreto de aplicação responsável por gerar um fluxo de dados em que é necessário realizar classificações em tempo real. Durante a etapa de classificação, este sensor exige a adaptação a possíveis variações em condições ambientais, responsáveis por alterar o comportamento dos insetos ao longo do tempo. Para lidar com este problema, é proposto um Sistema com Múltiplos Classificadores que realiza a seleção dinâmica do classificador mais adequado de acordo com características de cada exemplo de teste. Em avaliações com mudanças pouco significativas nas condições ambientais, foi possível obter uma acurácia de classificação próxima de 90%, no cenário com múltiplas classes e, cerca de 95% para a identificação da espécie Aedes aegypti, considerando o treinamento com uma única classe. No cenário com mudanças significativas nos dados, foi possível obter 91% de acurácia em um problema com 5 classes e 96% para a classificação de insetos vetores de importantes doenças como dengue e zika vírus.
Many applications are able to generate data continuously over t ime in an ordered and uninterrupted way in a dynamic environment , called data streams. Among possible tasks that can be performed with these data, classification is one of the most prominent . Due to non-stationarity of the environment that generates the data, the features that describe the concepts of the classes can change over time. Thus, the classifiers that deal with data streams require constants updates in their classification models to maintain a stable accuracy over time. In the update phase, most of the approaches assume that after the classification of each example from the stream, their actual class label is available without any t ime delay (zero latency). Given the high label costs, it is more reasonable to consider that this delay could vary for the most portion of the data. In the more challenging case, there are applications with extreme latency, where in after the classification of the examples, heir actual class labels are never available to the algorithm. In this scenario, it is not possible to use traditional approaches. Thus, there is the need of new methods that are able to maintain a classification model updated in the absence of labeled data. In this thesis, besides to discuss the problem of latency to obtain actual labels in data stream classification problems, neglected by most of the works, we also propose two new algorithms to deal with extreme latency, called SCARGC and MClassification. Both algorithms are based on the use of clustering approaches to adapt to changes in an unsupervised way. The proposed algorithms are intuitive, simpleand showed superior or equivalent results in terms of accuracy and computation time compared to other approaches from literature in an evaluation on synthetic and real data. In addition to the advance in the state-of-the-art in the stream learning area, this thesis also presents contributions to an important technological application with social and public health impacts. Specifically, it was studied an optical sensor to automatically identify insect species by the means of the analysis of information coming from wing beat of insects. To describe the data, we conclude that the Mel-cepst ral coefficients guide to the best results among different evaluated digital signal processing techniques. This sensor is a concrete example of an applicat ion that generates a data st ream for which it is necessary to perform real-time classification. During the classification phase, this sensor must adapt their classification model to possible variat ions in environmental conditions, responsible for changing the behavior of insects. To address this problem, we propose a System with Multiple Classifiers that dynamically selects the most adequate classifier according to characteristics of each test example. In evaluations with minor changes in the environmental conditions, we achieved a classification accuracy close to 90% in a scenario with multiple classes and 95% when identifying Aedes aegypti species considering the training phase with only the positive class. In the scenario with considerable changes in the environmental conditions, we achieved 91% of accuracy considering 5 species and 96% to classify vector mosquitoes of important diseases as dengue and zika virus.

La faune des termites de Guyane française représente environ le quart des espèces connues d'Isoptera de la région des Guyanes [Guyane française, Surinam, Guyane britannique, Brésil amazonien et Venezuela]. En Gyane se trouve 3 familles des termites : Kalotermitidae, Rhinotermitidae et Termitidae. La majorité des espèces rencontrées en Guyane appartiennent à cette dernière famille. Nasutitermes y est largement dominant. Nos travaux démontrent que les termites sont sensibles aux variables environnementales. La diversité spécifique et l'abondance des termites sont deux fois plus importantes en forêt dense qu'en forêt secondaire et dix fois plus importantes que celles des forêts ouvertes, des savanes, des inselbergs et des zones anthropisées. La diminution de la diversité végétale, la fragmentation des forêts et l'utilisation de leurs ressources naturelles produisent un déséquilibre pouvant déboucher sur des perturbations écologiques, perceptibles au travers de la diversité des termites. La richesse spécifique des termites est un indicateur important pour l'estimation de la biodiversité
The termites species of French Guiana represents approximately a quarter of the known species of Isoptera of the area (French Guiana, Surinam, British Guiana, Amazonian Brazil and Venezuela). The Termitidae include the majority of the species and dominate the other two families (Kalotermitidae, Rhinotermitidae). The genus Nasutitermes is largely dominant in the family Termitidae. Our work shows that the termites are sensitive to the environmental variables. Species diversity and the abundance of termites in the dense forests are two times more than in the secondary forests. They are 10 times more important than those of the open forests, savannas, inselbergs and anthropized zones. The reduction in plant diversity, the fragmentation of the forests and the use of their natural resources produce an imbalance which can lead to ecological perturbation, which are perceptible through the diversity of the termites. The diversity and specific abundance of termites are significant indicators for estimating the biodiversity

Les trichogrammes, hymenopteres parasitoides d'ufs de nombreux insectes ravageurs, notamment des lepidopteres, sont couramment utilises en controle biologique. Notre travail a porte sur des trichogrammes captures au maroc en 1988. L'etude taxinomique est basee sur des criteres morphologiques, mixiologiques et biochimiques. Les resultats permettent de distinguer deux especes, t. Voegelei et t. Bourarachae. L'analyse de la variabilite genetique a ete realisee par l'etude de fratries et par la recherche de correlations entre meres et filles. Les variations observees pour la capacite d'infestation, la dispersion de l'infestation, la sex ratio et le taux d'emergence de la descendance possedent une base genetique. L'analyse du mode de transmission de la capacite d'infestation par des back-crosses successifs montre que ce caractere se transmet selon un mode matrocline. Les effets de l'insemination et du superparasitisme sont etudies. Les femelles vierges produisent des ufs haploides viables. Elles ont une capacite d'infestation moins importante et un comportement plus dispersif que les femelles inseminees. La capacite d'infestation et la dispersion sont reduites chez les femelles issues d'hotes superparasites, mais la capacite d'infestation sommee pour 2 femelles issues d'un meme hote est superieure a celle d'une femelle developpee isolement. L'organisation temporelle de l'activite locomotrice a ete abordee a l'aide de 2 methodes. Les males et les femelles sont exclusivement diurnes mais leurs profils d'activite sont differents. Les resultats sont discutes sur le plan fondamental, dans le but de comprendre les mecanismes impliques dans la relation hote-parasitoide, et sur le plan applique, dans la perspective d'une amelioration des performances des insectes auxiliaires utilises en controle biologique

La forêt atlantique brésilienne est un des points sensibles de biodiversité avec une richesse spécifique et des risques d’extinction élevés. Cette forêt est située le long de la côte atlantique brésilienne, s’étendant jusqu’au Paraguay vers le Sud et à l’Argentine dans l’intérieur des terres. Du fait des gradients longitudinal et altitudinal, de la géologie complexe et de la diversité des sols, cette forêt comprend une diversité exceptionnelle de paysages et d’écosystèmes qui ont permis à cette riche biodiversité de se développer. Cependant, cette dernière encourt des risques extrêmes d’extinction du fait des densités et des tailles de populations humaines locales les plus élevées en Amérique du Sud. La forêt atlantique est ainsi aujourd’hui réduite à moins de 5% de sa surface originelle, répartie dans des fragments épars. En dépit de cette richesse spécifique reconnue, beaucoup reste à comprendre au sujet de plusieurs composantes de la biodiversité et de leur origine. Parmi les groupes encore mal connus figurent en particulier les insectes. Dans le but de combler cette lacune, j’ai étudié dans cette thèse un genre de blatte endémique de la forêt atlantique, Monastria Saussure, 1864 (Blattodea, Blaberinae). Je me suis focalisé sur sa taxonomie, sa phylogéographie et sur la contribution des données de collections d’histoire naturelle à la modélisation de l’aire de distribution. L’étude de la taxonomie a consisté à entreprendre la révision du genre avec la re-description des espèces espèces déjà connues et la description de nouvelles espèces. Les descriptions des espèces connues étaient fort anciennes et la description (et redescription) a donc inclus la définition de nouveaux caractères, ainsi qu’une étude des genitalia. Des problèmes nomenclaturaux anciens ont été également résolus, une clé d’identification des espèces ainsi qu’une clé d’identification des larves des genres de Blaberinae endémiques de la forêt atlantique ont été construites. La deuxième étude concernait l’analyse de la diversification et de la distribution du genre Monastria dans la forêt atlantique brésilienne. Cette analyse a indiqué l’importance des impacts différentiels des changements de température durant le dernier maximum glaciaire entre les parties Nord et Sud de la forêt atlantique, ceci résultant dans le patron de distribution présent. La troisième étude est une évaluation de l’intérêt des données disponibles dans les collections d’histoire naturelle concernant Monastria pour inférer son aire de répartition en se basant sur des modèles de niches écologiques (ENM), et en utilisant les données issues de l’échantillonnage de terrain ciblé sur Monastria pour valider les résultats. Nous montrons ici que le lot de données des collections est biaisé dans l’espace environmental. Le sur-échantillonnage dans une classe de climat conduit à construire des modèles d’aires favorables plus restreints que ceux de la distribution réelle de Monastria. Ces biais augmentent donc la spécificité des modèles et réduisent leur sensibilité. Pour résoudre ce problème, nous avons conçu deux sortes d’analyse de raréfaction et montré que la suppression aléatoire de points dans la classe climatique la plus biaisée augmente de manière très efficace la sensibilité du modèle de niche climatique
The Brazilian Atlantic forest is one of the biodiversity hotspots with the richest species diversity and threat. It is located along the Brazilian Atlantic coast going south til Paraguay and Argentina in the interior of the continent. Due to its longitudinal and altitudinal gradients, complex geology and diversity of soils it harbors an enormous diversity of landscapes and ecosystems that gave rise to its rich biodiversity. However, this biodiversity is extremely threatened because this region is the one with the highest population size and density in south America. So, the Atlantic forest is now limited to less than 5% of its original surface and distributed in scattered fragments. Despite the recognized species richness, much remains to be known about several components of this biodiversity and their origin. Among the groups still poorly known are the insects. In order to contribute to bridge this gap, in this thesis I studied one genus of cockroach endemic from the Atlantic forest, Monastria Saussure, 1864 (Blaberidae, Blaberinae). I focused on the taxonomy, phylogeography and on the contribution of the data existing in natural history collections to model the distribution range. The study of the taxonomy consisted in the revision of the genus with the re-description of already known species and description of new ones. Since the known species were described very early, the description (and re-description) comprised the definition of new characters, and consideration paid to genitalia. In addition to that, old nomenclatural problems were solved, a key to species’ identification was provided, a key to the identification of nymphs of the genera of Blaberinae endemic to the Atlantic forest were provided. The second study was aimed to understand the diversification and distribution of the genus Monastria in the Brazilian Atlantic Forest. This analysis indicates the importance of differential impacts of shifts in temperature between the Southern and Northeastern part of the Atlantic forest in the Last Glacial Maximum for explaining the present pattern of distribution. The third study is an evaluation of the data concerning Monastria available in Natural History Collections for estimating its distribution range based on Ecological Niche Models (ENM), and using the data from the field work designed to assess the presence of Monastria to validate the results. Here we showed that the dataset is biased in the environmental space. This oversampling in a climate class leads to models with suitable areas much smaller than that of the real distribution of Monastria. These biases increase model’s specificity and reduced sensitivity. To overcome this problem, we designed two forms of rarefaction and showed deleting points at random in the most biased climate class is very powerful to increase the sensitivity of the ENM

Himatanthus tarapotensis (Apocynaceae) connu au Pérou comme "bellaco caspi", est un arbre répandu dans plusieurs types d’habitat de l’Amazonie péruvienne où il est très utilisé en médicine traditionnelle pour le traitement de certaines maladies. Les propriétés médicinales des espèces de Himatanthus sont liées notamment à la présence du terpène bioactif pluméricine. Le potentiel pharmacologique de la pluméricine et le succès reproductif de H. tarapotensis ont mené à la réalisation de cette étude afin d’explorer les patrons de production de ce composé le long des variations des gradients environnementaux déterminés par les différents types d’habitat où la plante pousse naturellement. Cependant, il existe une confusion autour de la délimitation des espèces du genre Himatanthus qui pousseraient au Pérou et les noms scientifiques qui doivent être utilisés. En conséquent une partie de cette étude a consisté en l’évaluation taxonomique du genre Himatanthus. Dans la première partie de la thèse est présentée une révision synoptique des espèces de Himatanthus de l'Amazonie péruvienne, avec la finalité d'introduire une stabilité taxonomique de ces espèces au Pérou. Trois espèces de Himatanthus se trouvent au Pérou : H. tarapotensis, H. phagedaenicus et H. revolutus, distinguées entre eux notamment par des traits reproductifs. Dans la seconde partie, les feuilles d’individus juvéniles de H. tarapotensis qui se développent sur deux types d’habitat ont été prélevées pour évaluer la production de pluméricine en fonction des facteurs environnementaux : type de sol, période de précipitations, et pression des insectes en tenant compte de la croissance de la plante. La production de pluméricine chez H. tarapotensis ne montre pas différences significatives en fonction des types de sol. Mais, il a été observé une variation temporelle de la concentration du composé corrélée positivement avec la pression des insectes, notamment avec la larve herbivore Isognathus leachii (Lepidoptera : Sphingidae) et négativement avec la croissance. En même temps, ces deux facteurs ont été corrélés avec la précipitation, ce qui suggère que l’intensité de pluie influencerait la production de pluméricine de manière indirecte. Le composé bioactif pluméricine pourrait avoir un patron de production déterminé par son rôle médiateur des interactions de H. tarapotensis avec son environnement
Himatanthus tarapotensis (Apocynaceae) known in Peru as "bellaco caspi", is a common tree from several habitats in the Peruvian Amazon, where is widely used in traditional medicine for the treatment of various diseases. Medicinal properties from species of Himatanthus have been mainly related to the presence of a bioactive terpene plumericin. The pharmacological potential of plumericin and reproductive success of H. tarapotensis gave rise to the present study to explore the production patterns of this compound in function of environmental gradients determined by the different habitat types where this species grows in nature. However, the delimitation of Himatanthus species has long been problematic, and much confusion remains as to which names should be used and which species delimitations should be adopted. Consequently, a part of this study was dedicated to a taxonomic revision of Himatanthus species occurring in Peru. Therefore, in chapter 1 is presented a synoptic revision of Himatanthus species from the Peruvian Amazon, with the aim of introducing taxonomic stability of those species in Peru. Three species of Himatanthus are present in Peru: H. tarapotensis, H. phagedaenicus and H. revolutus, distinguished among themselves mainly by reproductive traits. In chapter 2, leaves collected from juvenile individuals of H. tarapotensis growing on two Amazonian habitats were used to evaluate the plumericin production in relation to environmental factors: soil type, period of precipitation, and insects pressure, keeping in mind their relation with the growth patterns of the plant. Plumericin production in H. tarapotensis was not affected by differences in soil types. However, it was observed a temporal variation in the concentration of this compound correlated positively to the insects pressure, mainly with the herbivorous larva of Isognathus leachii (Lepidoptera: Sphingidae) and negatively to the growth pattern of the plant. These two factors were correlated to the rainfall, thus suggesting that precipitation would be affecting indirectly on plumericin production. The bioactive compound plumericin would have a production pattern determined by its mediator role in interactions phenomena of H. tarapotensis and its environment

Der Wiederaustrieb beernteter Pappeln in Kurzumtriebsplantagen erfolgt nicht selten stark verzögert. Ob und inwieweit hierbei der Große Rote Pappelblattkäfer eine Rolle spielt, wurde in einem Versuch mit zwei unterschiedlichen Dichten des Schadinsekts untersucht.

The effects of reafforestation with seedlings of spruce, birch, mountain-ash, beech and larch, partially in combination with liming and removal of the topsoil layer before planting, on the community of saprophagous invertebrates (lumbricids, enchytraeids, oribatids, collembolans) were examined. The study area was located on plateau sites of the eastern Ore Mountains (Saxonia, Germany). Investigations were carried out on study sites exposed to high sulphur dioxide immissions and reforested 3 respectively 10 years ago. Invertebrates were sampled by the extraction of soil cores and by the ?electro-octett-method? (lumbricids). Additionally, the ?minicontainer method? was used to investigate decomposition rates of different types of litter. On the control area, a diedback spruce stand, the decomposer community showed high adaptations to the acidic soil substrate and was dominated by few enchytraeid species. Long-termed loss of tree shelter, liming and topsoil removal before planting resulted in a severe decline of most saprophagous species. However, changes in invertebrate community pattern due to different plantations were not found. A comparison of leaf and needle litter decomposition rates resulted in the following order: birch (k = 0.50) > mountain-ash (0.40) > spruce (0.30) >> larch (0.12). Considering all investigated taxa, the metabolic decomposing capacity of the larch litter reached 39 %, that the spruce litter 54 % of the foliage litter results.
Anläßlich des kompletten Ausfalls der Fichte in den extrem immissionsbelasteten Kammlagen des Osterzgebirges (Sachsen) wurden im Rahmen eines interdisziplinären Verbundprojektes verschiedene Waldbaukonzepte hinsichtlich ihrer Einflüsse auf die saprophage Invertebratenfauna (Enchytraeidae, Lumbricidae, Oribatida, Collembola) untersucht. Zur Disposition standen Verfahren zur Waldneugründung (Kulturparzellen mit Reihenpflanzung von Birke, Eberesche oder Fichte; zusätzlich waldbaulich unbeeinflußte Sukzessionsparzellen) und zum Waldumbau (Kulturparzellen mit Reihenpflanzung von Buche oder Lärche nach Abtrieb und flächigem Oberbodenabschub). Ein absterbender Fichtenreinbestand in fortgeschrittener Auflösung diente als Referenz- bzw. Korrelationsmaßstab. Die Erfassung der Mesofauna erfolgte über die Extraktion von Stechrohrproben. Lumbriciden wurden mit der Elektrooktett-Methode aufgenommen. Neben der flächenbezogenen Datenerfassung wurden 1.200 mit Blattstreu von Birke, Eberesche, Fichte und Lärche befüllte Minicontainer über 19 Monate auf einer Sukzessionsparzelle exponiert, um substratspezifische Abbaugeschwindigkeiten zu ermitteln und faunistische Sukzessionsverläufe zu studieren. Die Referenzfläche zeichnete sich durch eine stark enchytraeendominierte Zersetzergemeinschaft aus, die deutliche Anzeichen einer kalkungsbedingten Überprägung erkennen ließ. Der Enchytraeenanteil an den potentiellen Umsatzleistungen der untersuchten Destruententaxa (berechnet über metabolische Äquivalenzwerte) lag bei über 90 %. Hiervon ausgehend zeigten die Zersetzergemeinschaften der Versuchsanlagen "Waldneugründung" und "Waldumbau" stark divergierende Entwicklungsrichtungen. Als ausschlaggebende Faktoren konnten Schirmverlust, Kalkung und Oberbodenbeseitigung wahrscheinlich gemacht werden. Dagegen waren Einflüsse der unterschiedlichen Kulturbaumarten kaum nachzuweisen. Die streuspezifischen Dekompositionsgeschwindigkeiten unterschieden sich wie folgt: Birke (k = 0,50) > Eberesche (0,40) > Fichte (0,30) >> Lärche (0,12). Diese vergleichsweise geringen Abbauraten stehen überwiegend mit dem rauhen Montanklima in Zusammenhang. Enchytraeen besiedelten alle Streutypen zügig, während Collembolen vor allem die Ebereschenstreu verzögert aufsuchten und mit geringster Dichte bevölkerten. Oribatiden zeigten die geringste Besiedlungsgeschwindigkeit und hatten wie die Enchytaeen in der Laubstreu signifikant höhere Wohndichten. Nur Steganacarus spinosus zeigte eine besondere Affinität für Nadelstreu. Das metabolische Leistungspotential des Destruentenbesatzes der Lärchenstreu erreichte 39 %, das der Fichtenstreu 54 % der Laubstreuresultate.

Les changements climatiques ont des impacts considérables sur le temps, les océans et les rivages, la vie sauvage. Ils amènent des problèmes désormais considérés comme majeurs par les gouvernements et organisations internationales. Ces efforts ont fourni un cadre à cette thèse, qui propose de procéder en boucle fermée de l’observation d’insectes ravageurs, avec des centaines de capteurs en réseau ("light traps"), au système d’information, et enfin à des décisions de lutte, manuelles ou automatiques. Le point d’appui pratique est la conception d’un système de comptage d’insectes proliférant dans les cultures de riz (BPH). L’abstraction que nous développons est celle d’une machine environnementale de grande taille, distribuée, qui capte et synthétise l’information, élabore des connaissances, et prend des décisions. Autour de cette abstraction, nous avons élaboré un système de vision "fisheye" effectuant le comptage des insectes. Nous proposons un système d’information géographique directement connecté au réseau de capteurs. Le couplage direct, "cyber-physique", entre les systèmes d’information et l’observation de l’environnement à échelle régionale est une nouveauté transposable, qui permet de comprendre et contrôler quantité d’évolutions
Climate changes bring problems related to nature evolutions. Global warming has an impact on sea level, weather patterns, and wild life. A number of national and international organizations are developing research programs in these directions, including threats on cultures and insect proliferation. Monitoring these phenomena, observing consequences, elaborating counteracted strategies are critical for the economy and society.The initial motivation of this work was the understanding of change impacts in the Mekong Delta region. From there, automatic observation tools were designed with a real time information system able to integrate environmental measures, then to support knowledge production.Tracking environment evolutions is distributed sensing, which can be the association of efficient sensors and radio communications, operated under the control of an information system. Sensing insects is very complex due to their diversity and dispersion. However, this is feasible in the case of intensive agricultural production as it is the case of rice, having a small number of pests. An automatic vision observatory is proposed to observe the main threats for the rice, as an evolution of manual light traps. Radio communication weaves these observatories into a network with connection to databases storing measures and possible counteractions. An example observatory has a fisheye camera and insect counting algorithms for the BPH practical case in Vietnam.By considering the observation system as an input for an abstract machine, and considering decision and actions taken as a possible control on the environment, we obtain a framework for knowledge elaboration that can be useful in lots of other situations

Orientador: Patrícia Jacqueline Thyssen
Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Muitos levantamentos faunísticos de dípteros que foram e têm sido realizados atualmente no Brasil são precários por relatarem a presença de espécimes até níveis taxonômicos superiores ao de espécie. Dentre uma série de dificuldades que contribuem para isso estão a ausência de chaves taxonômicas para certos grupos e a insuficiência na descrição de caracteres em algumas das chaves existentes. Assim, o avanço de quaisquer outros trabalhos que queiram investigar a biologia, ecologia ou dinâmica desses organismos na natureza ou em laboratório estará comprometido, já que não é possível associar tais estudos a categorias abstratas tal como 'morfotipos'. As chaves taxonômicas interativas, elaboradas com auxílio de programas computacionais, quando comparadas às convencionais podem ser extremamente vantajosas por apresentarem banco de imagens, glossário de termos técnicos, serem mais dinâmicas no processo de inserção de novos caracteres, além de permitirem ao usuário a seleção de qualquer caráter sem uma ordem pré-determinada, garantindo maior flexibilidade durante o processo de identificação. Para elaboração das chaves interativas usando a plataforma JAVA e software Lucid® foram levantados caracteres e estados de caracteres para 20 espécies de moscas (Diptera: Calliphoridae) registradas para o Brasil, quer sejam de importância médica, veterinária ou forense. A identificação de algumas espécies de califorídeos usando a plataforma computacional não foi significativamente mais rápida quando comparada a feita por meio das chaves dicotômicas tradicionais, porém o número de erros de diagnóstico foi menor entre os usuários de chaves interativas. De qualquer modo, a chave interativa ainda pode ser superior por apresentar opções tais como exclusão, seleção ou priorização de caracteres sem exigir que o usuário siga uma ordem específica ou pré-determinada. O desenvolvimento de quaisquer ferramentas que auxiliem na busca pela identificação de um organismo, principalmente entre estudantes e profissionais não taxonomistas, é ainda mais valioso por fornecer um melhor entendimento para o conhecimento da diversidade de determinados grupos e para a descoberta de espécies novas na natureza. O trabalho final encontra-se disponível e publicado na web
Abstract: Many of the dipteran faunistic surveys carried out in the past, and the ones being conducted at the present in Brazil are precarious, because they report on the presence of specimens at taxonomic levels higher than species. The reasons for that are the lack of taxonomic keys to certain groups, and insufficient descriptions of morphological characters in some of the extant keys. Thus, the success of any study that aims to investigate the biology, ecology, and dynamics of these organisms in nature or in laboratory will be damaged, because it is not possible to associate these studies with abstract categories such as 'morphotypes'. Interactive taxonomic keys, prepared with the aid of computer programs, can be extremely advantageous when compared to conventional keys, because they contain image banks, glossary of technical terms, are more dynamic in the process of adding new characters, and allow the selection of any character without a predetermined order, ensuring greater flexibility during the identification process. For the production of interactive keys using JAVA platform and Lucid¿ software, we determined characters and states of characters for 20 species of flies (Diptera: Calliphoridae) of medical, veterinary and forensic importance from Brazil. The identification process using a Java platform was not significantly faster when compared to that done by traditional dichotomous keys, but the number of diagnostic errors was lower among users of interactive keys. Anyway, the interactive key may be superior to present options such as deletion, selection or prioritization of characters without requiring the user to follow a specific or predetermined order. The development of any tools that can aid in the identification of an organism, especially for students and non-taxonomist professionals, is even more valuable by providing a better understanding on the diversity of certain groups and on the discovery of new species in nature. The final work will be available and posted on the web
Mestrado
Parasitologia
Mestre em Parasitologia

Die paläarktische omnivore Weichwanze Dicyphus errans Wolff (Heteroptera, Miridae, Bryocorinae) zeichnet sich durch ein ausgesprochen breites Spektrum an besetzten Wirtspflanzen und konsumierten Beutetieren aus. Über 150 von D. errans akzeptierte Pflanzenarten sind erstmalig belegt worden. Der erfolgreiche Verzehr von 15 Beute-tierarten wurde quantitativ nachgewiesen. Eine weitere Besonderheit der Wanze besteht in der Präferenz für glandulär behaarte Pflanzen. Die Weichwanze besetzt somit von vielen anderen Insekten gemiedene Nischen. Untersuchungen im Botanischen Garten der Technischen Universität Dresden (2000 bis 2002) ließen auf eine bemerkenswerte räuberische Aktivität und Anpassungsfähigkeit an unterschiedliche Habitate, Beutetiere und Klimabedingungen schließen. Die fortführenden Studien im Rahmen der vorliegenden Dissertation (2002 bis 2005) erbrachten umfassende Erkenntnisse über die Art D. errans im Hinblick auf deren Morphologie, Vermehrung und Haltung, Bionomie und Ökologie (Fekundität und Ontogenese in Abhängigkeit von ausgewählten Einflussfaktoren, Dormanz), Aufenthalt und Fortbewegung (räumliche Orientierung und strukturanalytische Studien zu den Interaktionen zwischen Wanze und Pflanzenoberflächen), den Nahrungserwerb (Verzehrleistung und Omnivorie) sowie Videodokumentationen der Lebensweise und des Verhaltens. Der Generalist und Opportunist D. errans integriert sich als Pflanzensaftsauger regulierendes Kompartiment neben echten Räubern und Parasitoiden in Biozönosen. Als omnivores Insekt übernimmt die Wanze eine intermediäre und supplementäre Position in Nahrungsnetzen. Die Lebensweise dieser Weichwanzenart erscheint sehr komplex. Die enge Assoziation mit Pflanzen tritt stark in den Vordergrund. Sie ist in jeglichen Betrachtungen von D. errans unbedingt zu berücksichtigen und bietet außerdem als ein Modellsystem ein spannendes Forschungsfeld im Hinblick auf die Adaptation partiell räuberisch lebender Insekten an behaarte Pflanzen.
The omnivorous mirid bug Dicyphus errans Wolff (Heteroptera, Miridae, Bryocorinae) lives on a wide range of host plants and feeds on various preys. Over 150 plant species accepted by the mirid bug have been identified. It was experimentally shown that 15 prey species are efficiently consumed. Another characteristical features of D. errans is that it preferes hairy plants. Thus, the mirid bug occupies unique niches avoided by many other insects. Investigations, carried out at the Botanical Garden of the Technical University of Dresden (2000-2002) gave evidence for a remarkable predatory activity and ability to adaptation to different habitats, preys and climate conditions. The studies presented in the dissertation (2002-2005) gave the comprehensive knowledge about the species D. errans, especially its morphology, rearing, bionomy and ecology (fecundity and ontogenesis depending on selected factors, dormancy), habitat preference and locomotion (spatial orientation and structural-analytical studies of the interactions between the bug and plant surfaces), foraging and food ingestion (predatory capacity and omnivory). In addition, video documentation of the modus vivendi and the behavior was performed. The generalist and opportunist D. errans lives together with predators and parasitoids and takes part in a biological control of phytophagous insects in biocenoses. The life history of this bug species appeared to be very complex. The close association to plants has to be considered. Dicyphus errans offers a model system for further research on omnivorous predatory insects connected with hairy plants.

Une alternative prometteuse à la lutte chimique pour la régulation des ravageurs de culture consiste à favoriser les populations de leurs prédateurs en jouant sur la structure du paysage agricole. L'identification de structures spatio-temporelles favorables aux ennemis naturels peut se faire par l'exploration de scénarios paysagers via une modélisation couplée de paysages et de dynamiques de population. Dans cette approche, les dynamiques de populations sont simulées sur des paysages virtuels aux propriétés structurales contrôlées, et l'observation des motifs de populations associés permet l'identification de structures favorables. La modélisation des dynamiques de populations repose cependant sur une connaissance fine des processus écologiques et de leur variabilité entre les différentes unités du paysage. L'état actuel des connaissances sur les mécanismes écologiques régissant les dynamiques des ennemis naturels de la famille des carabidés demeure l'obstacle majeur à la recherche in silico de scénarios paysagers favorables. La littérature sur les liens entre motifs de population de carabes et variables paysagères permet de formuler un ensemble d'hypothèses en compétition sur ces mécanismes. Réduire le nombre de ces hypothèses en analysant les convergences entre les motifs de population qui leur sont associés, et étudier la stabilité de ces convergences le long d'un gradient paysager apparaît comme une première étape nécessaire vers l'amélioration de la connaissance sur les processus écologiques. Dans une première partie, nous proposons une heuristique méthodologique basée sur la simulation de modèles de réaction-diffusion porteurs de ces hypothèses en compétition. L'étude des motifs de population a permis d'effectuer une typologie des modèles en fonction de leur réponse à une variable paysagère, via un algorithme de classification, réduisant ainsi le nombre d’hypothèses en compétition. La sélection de l'hypothèse la plus plausible parmi cet ensemble irréductible doit s'effectuer sur la base d'une observation des motifs de population sur le terrain. Cela implique que ces derniers soient caractérisés à des résolutions spatiales et temporelles suffisantes pour sélectionner une unique hypothèse parmi celles en compétition. Dans la deuxième partie, nous proposons une heuristique méthodologique permettant de déterminer a priori des stratégies d'échantillonnage maximisant la robustesse de la sélection d'hypothèses écologiques. Dans un premier temps, la simulation de modèles de réaction-diffusion représentatifs des hypothèses écologiques en compétition permet de générer des données biologiques virtuelles en tout point de l'espace et du temps. Ces données biologiques sont ensuite échantillonnées suivant des protocoles différant dans l'effort total d'échantillonnage, le nombre de dates, le nombre de points par unité d'espace et le nombre de réplicats de paysages. Les motifs des populations sont caractérisés à partir de ces échantillons. Le potentiel des stratégies d'échantillonnage est évalué via un algorithme de classification qui classe les modèles biologiques selon les motifs de population associés. L'analyse des performances de classification, i.e. la capacité de l'algorithme à discriminer les processus écologiques, permet de sélectionner un protocole d'échantillonnage optimal. Nous montrons également que la manière de distribuer l'effort d'échantillonnage entre ses composantes spatiales et temporelles est un levier majeur sur l'inférence des processus écologiques. La réduction du nombre d'hypothèses en compétition et l'aide à l'échantillonnage pour la sélection de modèles répondent à un besoin fort dans le processus d'acquisition de connaissances écologiques pour l'exploration in silico de scénarios paysagers favorisant des services écosystémiques. Nous discutons dans une dernière partie des implications de nos travaux et de leurs perspectives d'amélioration
A promising alternative to the chemical control of pests consists in favoring their natural enemies populations by managing the agricultural landscape structure. Identifying favorable spatio-temporal structures can be performed through the exploration of landscape scenarios using coupled models of landscapes and population dynamics. In this approach, population dynamics are simulated on virtual landscapes with controlled properties, and the observation of population patterns allows for the identification of favorable structures. Population modeling however relies on a good knowledge about the ecological processes and their variability within the landscape elements. Current state of knowledge about the ecological mechanisms underlying natural enemies’ of the carabid family population dynamics remains a major obstacle to in silico investigation of favorable landscape scenarios. Literature about the relationship between carabid population and landscape properties allows the formulation of competing hypotheses about these processes. Reducing the number of these hypotheses by analyzing the convergence between their associated population patterns and investigating the stability of their convergence along a landscape gradient appears to be a necessary tep towards a better knowledge about ecological processes. In a first step, we propose a heuristic method based on the simulation of reaction-diffusion models carrying these competing hypotheses. Comparing the population patterns allowed to set a model typology according to their response to the landscape variable, through a classification algorithm, thus reducing the initial number of competing hypotheses. The selection of the most likely hypothesis from this irreducible set must rely on the observation of population patterns on the field. This implies that population patterns are described with spatial and temporal resolutions that are fine enough to select a unique hypothesis among the ones in competition. In the second part, we propose a heuristic method that allows determining a priori sampling strategies that maximize the robustness of ecological hypotheses selection. The simulation of reaction-diffusion models carrying the ecological hypotheses allows to generate virtual population data in space and time. These data are then sampled using strategies differing in the total effort, number of sampling locations, dates and landscape replicates. Population patterns are described from these samples. The sampling strategies are assessed through a classification algorithm that classifies the models according to the associated patterns. The analysis of classification performances, i.e. the ability of the algorithm to discriminate the ecological processes, allows the selection of optimal sampling designs. We also show that the way the sampling effort is distributed between its spatial and temporal components is strongly impacting the ecological processes inference. Reducing the number of competing ecological hypotheses, along with the selection of sampling strategies for optimal model inference both meet a strong need in the process of knowledge improvement about the ecological processes for the exploration of landscape scenarios favoring ecosystem services. In the last chapter, we discuss the implications and future prospects of our work

Fibre reinforced plastics (FRP) are increasingly employed in structural parts of the automotive, aviation and aerospace as well as railway industries. For those applications a heavily loaded, as well as crash and safety relevant force translation component is developed. This Hybrid-Insert consists of SMC and a metal insert, and is based on modular assembly through standard elements. The galvanic insulation of the metal insert by the SMC provides an excellent corrosion protection. The couplingstrength of the metal insert moulded into the SMC fulfills the tensile requirements of a M10 10.9 screw fit by VDI 2230 standards. Additionally the component provides a high degree of energy absorption and a gradual failure process
Faserverstärkte Kunststoffe (FVK) werden zunehmend in Strukturbauteilen der Automobil-,der Luft- und Raumfahrt- sowie der Schienenfahrzeugindustrie eingesetzt. Für diese Anwendungen wird ein hochbelastetes sowie crash- und sicherheitsrelevantes Krafteinleitungselement entwickelt. Dieses Hybrid-Insert, bestehend aus SMC und einem Metalleinsatz, ist modular aus Standardkomponenten aufgebaut. Die galvanische Isolation des Metalleinsatzes durch das SMC bietet für diesen einen hervorragenden Korrosionsschutz. Die Verankerungsfestigkeit des Metalleinsatzes im SMC genügt den Anforderungen einer M10 10.9 Verschraubung nach VDI 2230. Zusätzlich zeichnet sich das Krafteinleitungselement durch eine hohe Energieabsorption und ein gutmütiges Versagen aus

A new predictive modelling technique called the fuzzy envelope model (FEM) is introduced. The technique can be used to predict potential distributions of organisms using presence-only locality records and a set of environmental predictor variables. FEM uses fuzzy logic to classify a set of predictor variable maps based on the values associated with presence records and combines the results to produce a potential distribution map for a target species. This technique represents several refinements of the envelope approach used in the BIOCLIM modelling package. These refinements are related to the way in which FEMs deal with uncertainty, the way in which this uncertainty is represented in the resultant potential distribution maps, and the way that these maps can be interpreted and applied. To illustrate its potential use in biogeographical studies, FEM was applied to predicting the potential distribution of three invasive alien plant species (Lantana camara L., Ricinus communis L. and Solanum mauritianum Scop.), and three native cicada species (Capicada decora Germar, Platypleura deusta Thun. and P. capensis L.) in South Africa, Lesotho and Swaziland. These models were quantitatively compared with models produced by means of the algorithm used in the BIOCLIM modelling package, which is referred to as a crisp envelope model (the CEM design). The average performance of models of the FEM design was consistently higher than those of the CEM design. There were significant differences in model performance among species but there was no significant interaction between model design and species. The average maximum kappa value ranged from 0.70 to 0.90 for FEM design and from 0.57 to 0.89 for the CEM design, which can be described as 'good' to 'excellent' using published ranges of agreement for the kappa statistic. This technique can be used to predict species' potential distributions that could be used for identifying regions at risk from invasion by alien species. These predictions could also be used in conservation planning in the case of native species.

Das Forschungsfeld Schwarmintelligenz, also die Anwendung des Verhaltens dezentraler selbstorganisierender Tierkollektive, im Kontext der Informatik hat eine Reihe von state-of-the-art Kontroll- und Optimierungsmechanismen hervorgebracht. Die Untersuchung selbstorganisierender biologischer Systeme fördert zum einen das Design neuer robuster und adaptiver Algorithmen. Zum anderen kann sie das Verständnis der Funktionalität von selbstorganisierenden Prinzipien, welche in der Natur auftreten, unterstützen. Diese Arbeit deckt beide zuvor beschriebenen Aspekte ab. Unter Verwendung von Modellen und Simulation werden offene Fragen bezüglich der Organisation und des Verhaltens von sozialen Insekten beleuchtet. Weiter werden Abstraktionen von selbstorganisierenden Konzepten, welche man bei sozialen Insekten findet, genutzt, um neue Methoden zur Optimierung zu entwickeln. Der erste Teil dieser Arbeit untersucht allgemeine Aspekte der Arbeitsteilung sozialer Insekten. Zuerst wird die Anpassungsfähigkeit von unterschiedlich großen Kolonien, bezüglich dynamischer Veränderungen in der Umwelt untersucht. Die Ergebnisse zeigen, dass die Fähigkeit einer Kolonie, auf Veränderung in der Umwelt zu reagieren, von der Koloniegröße beeinflusst wird. Ein weiterer Aspekt der Arbeitsteilung, welcher in dieser Arbeit untersucht wird, ist, inwieweit eine räumliche Verteilung von Aufgaben und Individuen einen Einfluss auf die Arbeitsteilung hat. Die Ergebnisse deuten an, dass soziale Insekten von einer räumlichen Trennung, der zu bewerkstelligenden Aufgaben profitieren, da eine solche Trennung die Produktivität der Kolonie erhöht. Das könnte erklären, warum eine räumliche getrennte Anordnung von Aufgaben und Individuen häufig in realen Kolonien sozialer Insekten beobachtet werden kann. Der zweite Teil dieser Arbeit untersucht verschiedene Aspekte von Selbstorganisation bei Honigbienen. Zunächst wird der Einfluss der räumlichen Verteilung von Nestplätzen auf die Nestplatzsuche der europäischen Honigbiene Apis mellifera untersucht. Die Ergebnisse legen nahe, dass die Nestplatzsuche eines Schwarms aktiv durch die Anordnung der Nestplätze in der Umwelt beeinflusst wird. Eine nestplatzreiche Umgebung kann den Prozess eines Schwarms, sich für einen Nestplatz zu entscheiden, stark behindern. Das könnte erklären, warum Honigbienenarten, die geringe Anforderungen an Nestplätze haben, was die Anzahl von potenziellen Nestplätzen natürlich erhöht, eine sehr ungenaue Form der Nestplatzsuche aufweisen. Ein zweiter Aspekt der Honigbienen, welcher untersucht wird, sind die Steuerungsmechanismen, die dem kollektiven Flug eines Bienenschwarms unterliegen. Zwei mögliche Führungsmechanismen, aktive und passive Führung, werden hinsichtlich ihrer Fähigkeit verglichen, die Flugeigenschaften eines echten Honigbienenschwarms zu reproduzieren. Die Simulationsergebnisse bestätigen aktuelle empirische Befunde und zeigen, dass aktive Führung in der Lage ist, Charakteristika fliegender Schwärme widerzuspiegeln. Bei passiver Führung ist das nicht der Fall. Eine Anwendung biologischer Konzepte im Bereich der Informatik wird anhand der Nestplatzsuche demonstriert. Diese ist ein natürlicher Optimierungsprozess, basierend auf einfachen Regeln. Erzielt wird eine lokale Optimierung, die es einem Schwarm ermöglicht, Nestplätze in einer bisher unbekannten Umgebung zu finden und aus diesen den besten Nestplatz zu wählen. Das ist die Motivation, Nestplatzsuche im Bereich der Optimierung anzuwenden. Hierfür wird zuerst das Optimierungspotenzial der biologischen Nestplatzsuche mit Hilfe eines biologischen Modells untersucht. Basierend auf der Nestplatzsuche wird ein abstrahiertes algorithmisches Schema, das so genannte „Bee Nest-Site Selection Scheme“ (BNSSS) entworfen. Basierend auf dem Schema wird der erste Nestplatzsuche inspirierte Optimierungsalgorithmus „Bee-Nest\\\''\\\'' für die Anwendung im Bereich von molekular Docking entwickelt. Im Vergleich zu anderen Optimierungsalgorithmen erzielt „Bee-Nest“ eine sehr gute Leistung.
The application in computer science of the behaviour found in decentralized self-organizing animal collectives -- also known as swarm intelligence -- has brought forward a number of state-of-the art control and optimization mechanisms. Further study of such self-organizing biological systems can foster the design of new robust and adaptive algorithms, as well as aid in the understanding of self-organizing processes found in nature. This thesis covers both of the aspects described above, namely the use of computational models to investigate open questions regarding the organization and behaviour of social insects, as well as using the abstraction of concepts found in social insects to generate new optimization methods. In the first part of this work, general aspects of division of labour in social insects are investigated. First the adaptiveness of different-sized colonies to dynamic changes in the environment is analysed. The findings show that a colony\\\''s ability to react to changes in the environment scales with its size. Another aspect of division of labour which is investigated is the extent to which different spatial distributions of tasks and individuals influence division of labour. The results suggest that social insects can benefit from a spatial separation of tasks within their environment, as this increases the colony\\\''s productivity. This could explain why a spatial organization of tasks and individuals is often observed in real social insect colonies. The second part of this work investigates several aspects of self-organization found in honeybees. First the influence of spatial nest-site distribution on the ability of the European honeybee Apis mellifera to select a new nest-site is studied. The results suggest that a swarm\\\''s habitat can influence its decision-making process. Nest-site rich habitats can obstruct a swarm\\\''s ability to choose a single site if all sites are of equal quality. This could explain why in nature honeybee species which have less requirements regarding a new nest-site have evolved a more imprecise form of nest-site selection than cavity-nesting species. Another aspect of honeybees which is investigated is the guidance behaviour in migrating swarms. Two potential guidance mechanisms, active and passive guidance, are compared regarding their ability to reproduce real honeybee swarm flight characteristics. The simulation results confirm previous empirical findings, as they show that active guidance is able to reflect a number of characteristics which can be observed in real moving honeybee swarms, while this is not the case for passive guidance. Nest-site selection in honeybees can be regarded as a natural optimization process. It is based on simple rules and achieves local optimization as it enables a swarm to decide between several potential nest-sites in a previously unknown dynamic environment. These factors motivate the application of the nest-site selection process to the problem domain of function optimization. First, the optimization potential of the biological nest-site selection process is studied. Then a general algorithmic scheme called ``Bee Nest-Site Selection Scheme\\\''\\\'' (BNSSS) is introduced. Based on the scheme the first nest-site inspired optimization algorithm ``Bee-Nest\\\''\\\'' is introduced and successfully applied to the domain of molecular docking.

In this paper we study the dynamics of task division in threshold reinforcement models of social insect societies. Our work extends other models in order to include several factors that influence the behavior of real insect colonies. Main extensions of our model are variable demands for work, age-dependent thresholds and finite life span of the individuals. It is shown how these factors influence the degree of task specialization of the individuals in a colony. Moreover, we show that the introduction of a threshold-dependent competition process between the individuals during task selection leads to the occurrence of specialists and differentiation between individuals as an emergent phenomenon that depends on the colony size. This result can help to explain the proximate mechanisms that lead to specialization in large insect colonies. Our results have implications for the fields of multi-agent systems, robotics, and nature inspired scheduling where threshold response models are used for control and regulation tasks.

Since the 1990s water quality monitoring projects using aquatic insects or macroinvertebrates as bioindication in Mongolia has mostly occurred in rivers drainage to the Arctic Ocean. They have been conducted to identify different anthropogentic stressors and impacts upon these running water ecosystems. However, there are still knowledge gaps and uncertainties concerning the research of these macroinvertebrates, in particular, a life cycle study of representative species are one such section of information missing. The specific aim for the study was to determine their life cycles and secondary production of selected species in the Kharaa River Basin, Mongolia, where these animals are exposed to harsh environment conditions. The main challenges for the research project were selecting the most suitable methods for use in the field sampling campaigns as well as establishing biomonitoring criteria for the target species under the extreme harsh climatic conditions. The research also sorts to address the pre-existing taxonomical identification problems. Consequently, a multi-habitat quantitative sampling method, and emergence traps type ‘Model week’ were selected. Five specific traits were chosen as selection criteria from the literature, where the life cycles of numerous species were investigated under comparable conditions to this study. Based on those five distinct criteria, a total of 18 species from EPT group (Ephemeroptera, Plecoptera, and Trichoptera) were selected for deeper analysis. This thesis provided the first quantitative results on the life cycle, production, growth rate and emergence of aquatic insects from Mongolia, to allow comparisons with studies in other regions using the same methods. However, it still needs more quantitative research of population dynamics for a wider range of species including fecundity, accurate development rates, mortality losses (e.g., due to predation), and food availability across environmental gradients of hydraulic conditions and substrate types. In conclusion, last not least it is essential to obtain knowledge especially about life cycle strategies of macroinvertebrates to identify the indicator-properties of single species and to predict re-colonisation potential of disturbed habitats and to evaluate the efficiency of management measures.

Climate-triggered forest disturbances are increasing either by drought or by other climate extremes. Droughts can change the structure and function of forests in long-term or cause large-scale disturbances such as tree mortality, forest fires and insect outbreaks in short-term. Traditional approaches such as dendroclimatological surveys could retrieve the long-term responses of forest trees to drought conditions; however, they are restricted to individual trees or local forest stands. Therefore, multitemporal satellite-based approaches are progressing for holistic assessment of climate-induced forest responses from regional to global scales. However, little information exists on the efficiency of satellite data for analyzing the effects of droughts in different forest biomes and further studies on the analysis of approaches and large-scale disturbances of droughts are required. This research was accomplished for assessing satellite-derived physiological responses of the Caspian Hyrcanian broadleaves forests to climate-triggered droughts from regional to large scales in northeast Iran. The 16-day physiological anomalies of rangelands and forests were analysed using MODIS-derived indices concerning water content deficit and greenness loss, and their variations were spatially assessed with monthly and inter-seasonal precipitation anomalies from 2000 to 2016. Specifically, dimensions of forest droughts were evaluated in relations with the dimensions of meteorological and hydrological droughts. Large-scale effects of droughts were explored in terms of tree mortality, insect outbreaks, and forest fires using field observations, multitemporal Landsat and TerraClimate data. Various approaches were evaluated to explore forest responses to climate hazards such as traditional regression models, spatial autocorrelations, spatial regression models, and panel data models. Key findings revealed that rangelands’ anomalies did show positive responses to monthly and inter-seasonal precipitation anomalies. However, forests’ droughts were highly associated with increases in temperatures and evapotranspiration and were slightly associated with the decreases in precipitation and surface water level. The hazard intensity of droughts has affected the water content of forests higher than their greenness properties. The stages of moderate to extreme dieback of trees were significantly associated with the hazard intensity of the deficit of forests’ water content. However, the stage of severe defoliation was only associated with the hazard intensity of forests’ greenness loss. Climate hazards significantly triggered insect outbreaks and forest fires. Although maximum temperatures, precipitation deficit, availability of soil moisture and forest fires of the previous year could significantly trigger insect outbreaks, the maximum temperatures were the only significant triggers of forest fires from 2010‒2017. In addition to climate factors, environmental and anthropogenic factors could control fire severity during a dry season. The overall evaluation indicated the evidence of spatial associations between satellite-derived forest disturbances and climate hazards. Future studies are required to apply the approaches that could handle big-data, use the satellite data that have finer wavelengths for large-scale mapping of forest disturbances, and discriminate climate-induced forest disturbances from those that induced by other biotic and abiotic agents.
Klimagbedingte Waldstörungen nehmen entweder durch Dürre oder durch andere Klimaextreme zu. Dürren können langfristig die Struktur und Funktion der Wälder verändern oder kurzfristig große Störungen wie Baumsterben, Waldbrände und Insektenausbrüche verursachen. Traditionelle Ansätze wie dendroklimatologische Untersuchungen könnten die langfristigen Reaktionen von Waldbäumen auf Dürrebedingungen aufzeigen, sie sind aber auf einzelne Bäume oder lokale Waldbestände beschränkt. Daher werden multitemporale satellitengestützte Ansätze zur ganzheitlichen Bewertung von klimabedingten Waldreaktionen auf regionaler bis globaler Ebene weiterentwickelt. Es gibt jedoch nur wenige Informationen über die Effizienz von Satellitendaten zur Analyse der Auswirkungen von Dürren in verschiedenen Waldbiotopen. Daher sind weitere Studien zur Analyse von Ansätzen und großräumigen Störungen von Dürren erforderlich. Diese Forschung wurde durchgeführt, um die aus Satellitendaten gewonnenen physiologischen Reaktionen der im Nordosten Irans gelegenen kaspischen hyrkanischen Laubwälder auf klimabedingte Dürren auf lokaler und regionaler Ebene zu bewerten. Auf der Grundlage der aus MODIS-Daten abgeleiteten Indizes wurden die 16-tägigen physiologischen Anomalien von Weideland und Wäldern in Bezug auf Wassergehaltsdefizit und Grünverlust analysiert und ihre Variationen räumlich mit monatlichen und intersaisonalen Niederschlagsanomalien von 2000 bis 2016 bewertet. Insbesondere wurden die Dimensionen der Walddürre in Verbindung mit den Dimensionen der meteorologischen und hydrologischen Dürre bewertet. Großräumige Auswirkungen von Dürren wurden in Bezug auf Baumsterblichkeit, Insektenausbrüche und Waldbrände mit Hilfe von Feldbeobachtungen, multitemporalen Landsat- und TerraClimate Daten untersucht. Verschiedene Ansätze wurden ausgewertet, um Waldreaktionen auf Klimagefahren wie traditionelle Regressionsmodelle, räumliche Autokorrelationen, räumliche Regressionsmodelle und Paneldatenmodelle zu untersuchen. Die wichtigsten Ergebnisse zeigten, dass die Anomalien von Weideland positive Reaktionen auf monatliche und intersaisonale Niederschlagsanomalien aufweisen. Die Dürren in den Wäldern waren jedoch in hohem Maße mit Temperaturerhöhungen und Evapotranspiration verbunden und standen in geringem Zusammenhang mit dem Rückgang von Niederschlägen und des Oberflächenwasserspiegels. Die Gefährdungsintensität von Dürren hat den Wassergehalt von Wäldern stärker beeinflusst als die Eigenschaften ihres Blattgrüns. Die Stufen mittlerer bis extremer Baumsterblichkeit waren signifikant mit der Gefährdungsintensität des Defizits des Wassergehalts der Wälder verbunden. Das Ausmaß der starken Entlaubung hing jedoch nur mit der Gefährdungsintensität des Grünverlustes der Wälder zusammen. Die Klimagefahren haben zu deutlichen Insektenausbrüchen und Waldbränden geführt. Obwohl Maximaltemperaturen, Niederschlagsdefizite, fehlende Bodenfeuchte und Waldbrände des Vorjahres deutlich Insektenausbrüche auslösen konnten, waren die Maximaltemperaturen die einzigen signifikanten Auslöser von Waldbränden von 2010 bis 2017. Neben den Klimafaktoren können auch umweltbedingte und anthropogene Faktoren den Schweregrad eines Brandes während einer Trockenzeit beeinflussen. Die Gesamtbewertung zeigt Hinweise auf räumliche Zusammenhänge zwischen aus Satellitendaten abgeleiteten Waldstörungen und Klimagefahren. Weitere Untersuchungen sind erforderlich, um Ansätze anzuwenden, die mit großen Datenmengen umgehen können, die Satellitendaten in einer hohen spektralen Auflösung für die großmaßstäbige Kartierung von Waldstörungen verwenden und die klimabedingte Waldstörungen von denen zu unterscheiden, die durch andere biotische und abiotische Faktoren verursacht werden.

In dieser Arbeit werden Berechnungsvorschriften für Insertverbindungen in Holzfurnierlagenverbundwerkstoffen (WVC) mit dem Ziel erstellt, diese zur Auslegung und Nachweisführung in Anwendungen des Maschinenbaus zu verwenden. Nach Definition eines entsprechenden Anforderungsprofils erfolgt eine umfangreiche Darstellung des Standes der Technik. Dabei wird speziell auf die rechnerische Nachweisführung von Verbindungen im Bauwesen eingegangen, schwerpunktmäßig durch die im Eurocode 5 (DIN EN 1995) definierten Modelle für stiftförmige Verbindungsmittel. Im zweiten, praktischen Teil der Arbeit erfolgt die Durchführung und Auswertung umfangreicher Versuche. Dabei werden in einem ersten Schritt wesentliche Materialkennwerte der verwendeten Holzwerkstoffe bestimmt. Die zweite Gruppe der experimentellen Untersuchungen hat das Trag- und Verformungsverhalten vollständiger Insertverbindungen zum Inhalt. Dabei werden die unter statischen Aspekten wichtigen Einflussparameter auf die Tragfähigkeit bei Belastungen in Richtung sowie quer zur Stiftachse bestimmt und geometrische Vorzugsparameter ermittelt. Unter deren Zugrundelegung erfolgt eine Ausweitung der experimentellen Untersuchungen auf dynamische Lastfälle durch eine Ableitung von Wöhlerlinien bei Zugschwellbelastung. Auf Grundlage der durchgeführten Versuche werden Modelle zur Berechnung von Insertverbindungen nach den Methoden des Ingenieurholzbaus abgeleitet. Deren Anwendbarkeit wird an einem konkreten Beispiel in Form einer Transportrollenbahn für die Verwendung in der technischen Intralogistik demonstriert.
In this work, calculation fundamentals for insert fastenings in wood veneer composites (WVC) are developed with the aim to use them for the dimensioning and verification in mechanical engineering applications. After defining the functional requirements there is a comprehensive description of the best demonstrated available technology. The arithmetical verifications of connections used in civil engineering are thereby specifically addressed with the main focus on defined models for dowel type fasteners within Eurocode 5 (EN 1995). In the second part of the work comprehensive technical tests are implemented and evaluated. At first essential material properties of the used wood materials are determined. The following experiments contain the load bearing and deformation behavior of complete insert connections. The determination of important influencing parameters of the statical strength under axial and traverse loads is thereby involved, followed by an extension on dynamic load cases by a derivative of Woehler curves (stress - cycle diagram). Based on these experiments are models derived to provide calculation fundamentals to dimension insert connections. The practical applicability of the developed specifications is shown by dimensioning the connections of a roller conveyor made of WVC (technical logistics).

Die Prävalenz einer Insektengiftallergie beträgt 2,8 %. Am häufigsten sind Honigbienen (Apis melliferi) und Faltenwespen (Vespula vulgaris, Vespula germanica) Auslöser einer Insektengiftallergie in Deutschland. Sie ist eine allergische Typ-I-Reaktion und durch eine Immunglobulin-E-vermittelte (IgE) Immunreaktion charakterisiert. IgE führt zur Sensibilisierung der an einer Immunreaktion beteiligten Mastzellen und basophilen Granulozyten. Durch den Zweitkontakt erfolgt die Aktivierung jener mit Degranulation von Histamin, Serinproteasen, Prostaglandinen, Leukotrienen und Zytokinen. Dies spiegelt sich in einer allergischen Reaktion mit Vasodilatation, Tachykardie, Hypotonie, Bronchokonstriktion, Pruritus, Schmerzen, Erythem oder Flush, Nausea, Vomitus und Diarrhoe wieder. Mittels Hauttests wie Prick- und Intrakutantest kann eine IgE-vermittelte Sensibilisierung auf das Insektengiftallergen nachgewiesen werden. Es werden die Serumparameter Gesamt-IgE und spezifisches IgE auf native und rekombinante Allergene des Insektengiftes gemessen. Der Basophile Aktivierungstest (BAT) kann ergänzt werden. Hierbei wird die immunologische Quantifizierung der Rezeptorenaktivität auf basophilen Granulozyten mittels Detektion der membranständigen Aktivierungsmarker CD63 und CD203c vor und nach Antigenexposition gemessen. CD63 befindet sich intragranulär gespeichert in ruhenden basophilen Granulozyten, Mastzellen, Makrophagen und Monozyten. In vitro konnte eine verstärkte Expression von CD63 v.a. allergen-induziert und FcεRI-vermittelt beobachtet werden. CD203c ist ein hochspezifischer Marker für die basophile Differenzierungslinie. Nach Allergenstimulation wird eine rasche Expression von CD203c beobachtet, welche FcεRI-vermittelt ist. Am Ausmaß der Expression von CD63 und CD203c kann die allergene Eigenschaft abgeleitet und bei Kreuzreaktivität i.R. der in-vitro-Diagnostik das auslösende Allergen identifiziert werden. Die spezifische Immuntherapie (SIT) ist die einzige kausale Therapie einer Insektengiftallergie. Pathophysiologisch wird eine immunmodulatorische Wirkung angenommen. Die Stichprovokation schätzt die Therapieeffektivität einer SIT auf Grund von Mangel an validen laborchemischen Kriterien ein. Bei Asymptomatik oder lokaler allergischer Reaktion hat das Ergebnis einen hohen prädiktiven Wert für die Verträglichkeit weiterer Stiche. Bei ausbleibender systemischer Reaktion nach 3 bis 5 Jahren SIT kann jene beendet werden, wenn SIT oder Stichprovokation ohne Nebenwirkungen vertragen wurden. Ein Feldstich ist der Stichprovokation ebenbürtig, wenn das allergieauslösende Insekt sicher identifiziert wurde. Nach SIT kommt es bei bis zu 15% der Patienten zum Verlust des Schutzes nach 5-10 Jahren (Epidemiology of Insect Venom Sensitivity | JAMA | The JAMA Network, 2017). Die Leitlinie empfiehlt daher ein Notfallset dauerhaft mitzuführen. Vor einer Stichprovokation muss eine 109 Risiko-Nutzen-Abwägung bei relevanten Nebenerkrankungen oder vorbekannter Mastozytose erfolgen. Da die Aktivität des Insektes sowie die Giftzusammensetzung in Abhängigkeit der Jahreszeit variieren, geht die Stichprovokation mit einer eingeschränkten Beurteilbarkeit einher. Auf Grund von erhöhter Angst und Arbeitsausfall für bis zu 3 Tage wird eine Provokation häufig nicht durchgeführt. Daher besteht die Notwendigkeit nach Messmethoden, welche die Therapieeffektivität einer SIT sicher beurteilen. Ziel der vorliegenden Arbeit ist den BAT bezüglich seines Nutzens als Monitoringinstrument während einer SIT zu prüfen und die Therapieeffektivität jener beurteilen zu können. Es wurden 50 Probanden während einer SIT begleitet (6 Kinder mit 4x Bienengift- und 2x Wespengiftallergie, 44 Erwachsene mit 4x Bienengift- und 40x Wespengiftallergie). Die Probanden erklärten sich zu Blutentnahmen vor der SIT und alle 6 Monate bis 3 Jahre während der SIT einverstanden. Hieraus erfolgte die Bestimmung der Serumparameter mittels des ImmunoCAP® 250 der Firma Thermo Fisher Scientific/ Phadia. Mit Hilfe des Flow CAST® Kit (FK-CCR) von der Firma Bühlmann Laboratories AG wurde die Expression der Oberflächenmarker basophiler Granulozyten CD63 und CD203c im BAT gemessen. Anhand des a2-Wertes wurde der relative Anteil an aktivierten basophilen Granulozyten [%] auf die Stimulation mit der Allergenkonzentration von 56,8 ng/ml (c2) bestimmt. Der kalkulierte c50-Wert definiert die mindest notwendige Konzentration an Allergen, um eine Aktivierung von 50% aller in der Testprobe vorliegenden basophilen Granulozyten zu induzieren. Klinische Daten wurden in halbjährlichen Visiten telefonisch, vor Ort, mittels Fragebogen und durch Einsicht in Patientenakten erhoben. Probanden wurden nach Stichprovokation oder Feldstich während SIT und Studie zu Verträglichkeit und Klinik befragt. 90,9% der Erwachsenen und 100% der Kinder boten eine allergische Reaktion Stadium II oder III nach Ring & Messmer. Anaphylaktische Reaktionen wurden nicht beschrieben oder beobachtet. Es waren 40 Erwachsene mit einer Wespengift-SIT zu verzeichnen. Für CD203c sind jene im BAT alle Responder, für CD63 waren 4 Probanden Nonresponder. Für CD63 zeigten sich für 60% steigende und für etwa 30% konstante c50-Werte i.V. der SIT. Dies spiegelt sich in fallenden a2-Werten bei etwa 60% aller Probanden dieser Gruppe wieder. Für CD203c konnten für 60-75% der Probanden steigende sowie für 20% konstante c50-Werte kalkuliert werden. Nach einem Jahr SIT boten 20%, nach zwei Jahren 32,5% und nach drei Jahren SIT 47,5% aller Probanden eine geringere Aktivierung basophiler Granulozyten. In der vorliegenden Studie konnte für 4 Testkonzentrationen (c1 = 284 ng/ml, c2 = 56,8 ng/ml, c3 =11,4 ng/ml sowie c4 = 2,27 ng/m) in allen untersuchten Studiengruppen (Kinder mit Bienengift-SIT, Erwachsene mit Bienengift-SIT, Kinder mit Wespengift-SIT und Erwachsene mit Wespengift-SIT) fallende am-Werte über die Zeit der SIT ermittelt werden. 110 Dies korrelierte mit steigenden c50-Werten über die Dauer der SIT. Die Routinetest-konzentration des Flow CAST® Kit (56,8 ng/ml) gibt hierbei die beste Diskriminierung wieder. Alle 40 Erwachsenen mit einer Wespengiftallergie beschrieben eine mildere klinische Symptomatik unter SIT. Für alle 21 Probanden, welche an einer Stichprovokation teilnahmen oder einen Feldstich erlitten, bestätigte sich dies im BAT. Anhand von Serologie oder Kinetikmessungen lässt sich in der vorliegenden Studie keine Aussage zur Immunmodulation einer SIT und ihrer Therapieeffektivität treffen. Der BAT hingegen ist ein mögliches valides Messverfahren, um die Effektivität einer SIT zu prüfen. Hierfür eignen sich bei guter Korrelation zu Klinik und Stichprovokation/ Feldstich der a2-Wert sowie der kalkulierte c50-Wert. Das Ergebnis eines BAT ist reproduzierbar. Es können Arbeitsausfall und ein erhöhtes Angstempfinden vermieden werden. Ein Routineeinsatz des BAT kann anhand der Studie noch nicht abgeleitet werden. Hausmann et. al konnten jedoch 2014 den c50-Wert als valides Monitoringinstrument der Effektivität bei guter Korrelation zur Stichprovokation belegen. Der BAT ist daher in Fällen interessant, in denen eine Stichprovokation nicht möglich ist (Kontraindikationen, Schwangerschaft, Patientenwunsch). Eine Langzeitwirkung der SIT könnte ggf. mit begleitenden BAT-Messungen geprüft werden. Damit ließe sich die Effektivität einer SIT zum Beispiel auch nach 10 oder 15 Jahren prüfen. Dann wäre eine Aussage darüber möglich, ob das Notfallset lebenslang indiziert ist. Weitere Studien sind notwendig, um die vorliegenden Ergebnisse zu stützen. Eine multizentrische Studie mit standardisiertem BAT und begleitender Stichprovokation ist hierfür Voraussetzung.:Abbildungsverzeichnis Tabellenverzeichnis Abkürzungsverzeichnis 1 Einleitung und Zielstellung 2 Hintergrund und Wissensstand 2.1. Insektengiftallergie 2.1.1. Terminologie der Allergie 2.1.2. Prävalenz der Insektengiftallergie 2.1.3. Hymenoptera - Arten, Taxonomie und Gifte 2.1.4. Immunologische Grundlagen der allergischen Reaktion 2.2. Diagnostik allergischer Reaktionen 2.2.1. Anamnese 2.2.2. Klinik 2.2.3. Hauttests 2.2.4. In-vitro-Allergiediagnostik 2.2.5. Zelluläre Testverfahren 2.3. Therapie 2.3.1. Allgemeine Maßnahmen 2.3.2. Notfalltherapie 2.3.3. Spezifische Immuntherapie (SIT) nach aktueller Leitlinie 2.3.4. Stichprovokation 3 Material und Methoden 3.1. Patientenkollektiv 3.2. Blutentnahmen im Rahmen der Studie während der SIT 3.3. Serologische Untersuchungen 3.4. Zelluläre Testverfahren 3.4.1. Basophiler Aktivierungstest (BAT) 3.4.2. Kinetikuntersuchungen 3.5. Stichprovokation und Feldstiche 3.6. Anamnestische Datenerhebung 3.7. Statistische Methoden 4 Ergebnisse 4.1. Studienpopulation 4.2. Stichereignisse 4.3. Aktivierung basophiler Granulozyten im BAT 4.3.1. Aktivierung basophiler Granulozyten im BAT - c50 und a2 4.3.2. Zeitlicher Verlauf der mittleren Aktivierung basophiler Granulozyten während SIT, Dosis-Wirkungs-Kurve 4.4. Verlauf der serologischen Messdaten 4.5. Kinetikuntersuchungen im BAT 4.6. Anamnese und Klinik 4.7. Korrelation BAT-Ergebnisse und Anamnese/ Klinik 4.8. Korrelation BAT-Ergebnisse und Stichprovokationen/ Feldstiche 5 Diskussion 5.1. Prävalenz der Insektengiftallergie und Verteilungsmuster der allergischen Reaktion nach Ring und Messmer 5.2. Diagnostik und Therapie einer Insektengiftallergie, Studienpopulation 5.3. State of the art - Lücken in Diagnostik und Therapie einer Insektengiftallergie 5.4. Alternative Methoden der Beurteilung der Effektivität einer SIT 5.5. Aktivierung basophiler Granulozyten im BAT 5.5.1 Aktivierung basophiler Granulozyten BAT - a2 und c50 5.5.2. Kinetik der Immunmodulation unter SIT 5.5.3. Interleukin-3 und Expressionskinetik der Oberflächenmarker CD63/ CD203c 5.5.4. Responder versus Nonresponder 5.5.5. Korrelation BAT-Ergebnisse und Anamnese/ Klinik 5.5.6. Korrelation BAT-Ergebnisse und Stichprovokation 5.6. Verlauf der serologischen Messdaten 5.7. Fehleranalysen 5.8. Ausblick in die zukünftige Forschung 6 Zusammenfassung 7 Literaturverzeichnis 8 Danksagung Anhang A Curriculum vitae Anhang B. Veröffentlichungen Anhang C. Anlage 1 - Eröffnung Promotionsverfahren Anhang D. Anlage 2 - Einhaltung gesetzlicher Vorgaben Anhang E. Anlage 3 - Eidesstattliche Erklärung Anhang F. Anlage 4 - Fragebogen Anhang G. Anlage 5 - Experimentelle Daten

Ausgehend von der Anwendung von Folienhinterspritzprozessen für dekorative Zwecke wurde deren Nutzung für die Integration elektronischer Strukturen in Kunststoffbauteile untersucht. Die Herstellung der elektronischen Bauelemente erfolgte mittels verschiedener Druckverfahren mit elektrisch leitfähigen und dielektrischen Materialien auf Polycarbonatfolien. Im Fokus standen zum einen kapazitiv auslesbare Speicherstrukturen und zum anderen Elektrolumineszenzleuchten. Nach dem Druck wurden die bedruckten Folien z. T. verformt und hinterspritzt. In der Arbeit wird auf die Auswirkungen der Verform- und Hinterspritzprozesse eingegangen. Schwerpunktmäßig wird die elektronische bzw. optische Funktionalität der Bauelemente, die Beeinflussung durch Klimaveränderungen und die Haftfestigkeit der Folien betrachtet. Im Ergebnis konnten erstmals die Realisierbarkeit hinterspritzter elektronischer Bauelemente nachgewiesen sowie verschiedene Einflussfaktoren auf deren Funktionalität identifiziert werden.
Based on the application of film insert molding for graphic purposes, the utilization of this process for the integration of electronic devices into plastic components was examined. The manufacturing of the electronic devices was realized by applying electrical conductive and dielectric inks on polycarbonate foil by various printing processes. Capacitive data storage patterns and electroluminescent lamps are the main applications. The production sequence included the printing process, forming of the foil where required and back injection molding. The impact of forming and film insert molding was investigated. The electronic and/or optical functionality of the devices, the influence of ambient conditions like temperature or humidity and the adhesion strength of the foils were in the focus of the evaluation. As a result, the feasibility of film insert molded electronic devices could be verified and various impact factors could be identified for the first time.