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1
Wu, Xiujuan, and Jian Ye. "Manipulation of Jasmonate Signaling by Plant Viruses and Their Insect Vectors." Viruses 12, no. 2 (January 27, 2020): 148. http://dx.doi.org/10.3390/v12020148.
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Plant viruses pose serious threats to stable crop yield. The majority of them are transmitted by insects, which cause secondary damage to the plant host from the herbivore-vector’s infestation. What is worse, a successful plant virus evolves multiple strategies to manipulate host defenses to promote the population of the insect vector and thereby furthers the disease pandemic. Jasmonate (JA) and its derivatives (JAs) are lipid-based phytohormones with similar structures to animal prostaglandins, conferring plant defenses against various biotic and abiotic challenges, especially pathogens and herbivores. For survival, plant viruses and herbivores have evolved strategies to convergently target JA signaling. Here, we review the roles of JA signaling in the tripartite interactions among plant, virus, and insect vectors, with a focus on the molecular and biochemical mechanisms that drive vector-borne plant viral diseases. This knowledge is essential for the further design and development of effective strategies to protect viral damages, thereby increasing crop yield and food security.
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Zongoma, A. M., D. B. Dangora, M. Sétamou, M. D. Alegbejo, and O. J. Alabi. "Identification of mealybugs, soft scale insects and their predators in vineyards across the savannah agro-ecological region of Nigeria." Zoologist (The) 18, no. 1 (April 8, 2021): 27–32. http://dx.doi.org/10.4314/tzool.v18i1.5.
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Insect-vectored viruses are a major threat to grapevine production but there is a dearth of information on the occurrence and distribution of key grapevine pests in Nigeria. The recent detection of grapevine leafroll associated virus-1 (GLRaV-1), a known insect-vectored ampelovirus, in Nigeria elevates the importance of the identification of its potential vectors as a precursor to assessing the risk of grapevine leafroll disease spread. This study was conducted to determine the occurrence and diversity of potential vectors of grapevine viruses and their natural enemies in vineyards across the savannah agro-ecological region of Nigeria. Forty vineyard and nursery locations were surveyed during 2016 and 45 arthropod samples were collected. The samples were first morphologically identified, and DNA barcoding was conducted on a subset of 16 representative samples using universal primers specific to the Mitochondrial Cytochrome Oxidase subunit I (mtCOI) gene of most insects. The results indicated the presence of two species of scale insects (Parasaissetia nigra and Saissetia coffeae) and two mealybug species (Maconellicoccus hirsutus and Ferrisia virgata), some ofwhich are potential grapevine virus vectors, in Nigerian vineyards. In addition, the natural enemies of these insect species were detected which includes three species of parasitoids (Anagyrus kamali, Anagyrus pseudococci and Encarsia inaron) and one predator (Hyperaspidius mimus). While the detection of mealybugs and scale insects underscore the risk of vector-mediated virus spread in Nigerian vineyards, the identification of their natural enemies indicates presence of natural biological control agents to facilitate an integrated management of economically important grapevine virus diseases in the country.
Keywords: Mealybugs; scale insects; parasitoids and predators; insect vectors; grapevine viruses.
3
Madden, L. V., M. J. Jeger, and F. van den Bosch. "A Theoretical Assessment of the Effects of Vector-Virus Transmission Mechanism on Plant Virus Disease Epidemics." Phytopathology® 90, no. 6 (June 2000): 576–94. http://dx.doi.org/10.1094/phyto.2000.90.6.576.
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A continuous-time and deterministic model was used to characterize plant virus disease epidemics in relation to virus transmission mechanism and population dynamics of the insect vectors. The model can be written as a set of linked differential equations for healthy (virus-free), latently infected, infectious, and removed (postinfectious) plant categories, and virus-free, latent, and infective insects, with parameters based on the transmission classes, vector population dynamics, immigration/emigration rates, and virus-plant interactions. The rate of change in diseased plants is a function of the density of infective insects, the number of plants visited per time, and the probability of transmitting the virus per plant visit. The rate of change in infective insects is a function of the density of infectious plants, the number of plants visited per time by an insect, and the probability of acquiring the virus per plant visit. Numerical solutions of the differential equations were used to determine transitional and steady-state levels of disease incidence (d*); d* was also determined directly from the model parameters. Clear differences were found in disease development among the four transmission classes: nonpersistently transmitted (stylet-borne [NP]); semipersistently transmitted (foregut-borne [SP]); circulative, persistently transmitted (CP); and propagative, persistently transmitted (PP), with the highest disease incidence (d) for the SP and CP classes relative to the others, especially at low insect density when there was no insect migration or when the vector status of emigrating insects was the same as that of immigrating ones. The PP and CP viruses were most affected by changes in vector longevity, rates of acquisition, and inoculation of the virus by vectors, whereas the PP viruses were least affected by changes in insect mobility. When vector migration was explicitly considered, results depended on the fraction of infective insects in the immigration pool and the fraction of dying and emigrating vectors replaced by immigrants. The PP and CP viruses were most sensitive to changes in these factors. Based on model parameters, the basic reproductive number (R0)—number of new infected plants resulting, from an infected plant introduced into a susceptible plant population—was derived for some circumstances and used to determine the steady-state level of disease incidence and an approximate exponential rate of disease increase early in the epidemic. Results can be used to evaluate disease management strategies.
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Mubarik, Muhammad Salman, Sultan Habibullah Khan, Aftab Ahmad, Ali Raza, Zulqurnain Khan, Muhammad Sajjad, Reda Helmy Ahmed Sammour, et al. "Controlling Geminiviruses before Transmission: Prospects." Plants 9, no. 11 (November 12, 2020): 1556. http://dx.doi.org/10.3390/plants9111556.
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Whitefly (Bemisia tabaci)-transmitted Geminiviruses cause serious diseases of crop plants in tropical and sub-tropical regions. Plants, animals, and their microbial symbionts have evolved complex ways to interact with each other that impact their life cycles. Blocking virus transmission by altering the biology of vector species, such as the whitefly, can be a potential approach to manage these devastating diseases. Virus transmission by insect vectors to plant hosts often involves bacterial endosymbionts. Molecular chaperonins of bacterial endosymbionts bind with virus particles and have a key role in the transmission of Geminiviruses. Hence, devising new approaches to obstruct virus transmission by manipulating bacterial endosymbionts before infection opens new avenues for viral disease control. The exploitation of bacterial endosymbiont within the insect vector would disrupt interactions among viruses, insects, and their bacterial endosymbionts. The study of this cooperating web could potentially decrease virus transmission and possibly represent an effective solution to control viral diseases in crop plants.
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Di Mattia, Jérémy, Faustine Ryckebusch, Marie-Stéphanie Vernerey, Elodie Pirolles, Nicolas Sauvion, Michel Peterschmitt, Jean-Louis Zeddam, and Stéphane Blanc. "Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector." Viruses 12, no. 3 (March 10, 2020): 299. http://dx.doi.org/10.3390/v12030299.
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Single-stranded DNA (ssDNA) plant viruses belong to the families Geminiviridae and Nanoviridae. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner. While geminiviruses are transmitted by leafhoppers, treehoppers, whiteflies and aphids, nanoviruses are transmitted exclusively by aphids. Circulative transmission involves complex virus–vector interactions in which epithelial cells have to be crossed and defense mechanisms counteracted. Vector taxa are considered a relevant taxonomic criterion for virus classification, indicating that viruses can evolve specific interactions with their vectors. Thus, we predicted that, although nanoviruses and geminiviruses represent related viral families, they have evolved distinct interactions with their vector. This prediction is also supported by the non-structural Nuclear Shuttle Protein (NSP) that is involved in vector transmission in nanoviruses but has no similar function in geminiviruses. Thanks to the recent discovery of aphid-transmitted geminiviruses, this prediction could be tested for the geminivirus alfalfa leaf curl virus (ALCV) and the nanovirus faba bean necrotic stunt virus (FBNSV) in their common vector, Aphis craccivora. Estimations of viral load in midgut and head of aphids, precise localization of viral DNA in cells of insect vectors and host plants, and virus transmission tests revealed that the pathway of the two viruses across the body of their common vector differs both quantitatively and qualitatively.
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Mellor, P. S., J. Boned, C. Hamblin, and S. Graham. "Isolations of African horse sickness virus from vector insects made during the 1988 epizootic in Spain." Epidemiology and Infection 105, no. 2 (October 1990): 447–54. http://dx.doi.org/10.1017/s0950268800048020.
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SUMMARYThis paper describes the first isolations of African horse sickness virus (AHSV) from insects in Spain. Seven isolations of AHSV serotype 4 were made; four from Culicoides imicola a known vector of the virus elsewhere, two from mixed pools of Culicoides species not including C. imicola and one from blood engorged mosquitoes. Three further isolations of AHSV serotype 4 were also made from horses kept adjacent to the insect collecting sites.This work presents the first definitive identification of the vectors of AHSV in Spain during the 1987, 88 and 89 epizootics. Suggestions are also made concerning the significance of these findings with regard to the epidemiology of African horse sickness in Spain.
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Moya Fernández, Marcia Beatriz, Wenwen Liu, Lu Zhang, Jamal-U.-Ddin Hajano, and Xifeng Wang. "Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector." Viruses 13, no. 6 (June 10, 2021): 1121. http://dx.doi.org/10.3390/v13061121.
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Plant viruses transmitted by hemipteran vectors commonly cause losses to crop production. Rice stripe virus (RSV) and rice black streaked dwarf virus (RBSDV) are transmitted to rice plants by the same vector, the small brown planthopper (SBPH), Laodelphax striatellus Fallén, in a persistent propagative manner. However, rarely do the respective diseases they cause occur simultaneously in a field. Here, we determined the acquisition efficiency of RSV and RBSDV when acquired in succession or simultaneously by SBPH. When RBSDV was acquired first, RSV acquisition efficiency was significantly lower than when only acquiring RSV. However, RBSDV acquisition efficiency from insects that acquired RSV first was not significantly different between the insects only acquiring RBSDV. Immunofluorescence assays showed that the acquisition of RBSDV first might inhibit RSV entry into midgut epithelial cells, but RSV did not affect RBSDV entry. SBPHs were more likely to acquire RBSDV when they were feeding on plants coinfected with the two viruses. When RBSDV was acquired before RSV, RBSDV titer was significantly higher and RSV titer first declined, then increased compared to when only acquiring RBSDV or RSV. Only 5% of the SBPHs acquired both viruses when feeding on plants coinfected with RSV and RBSDV. These results provide a better understanding of the interaction between two persistent viruses when present in the same vector insect and explain why RSV and RBSDV occur in intermittent epidemics.
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Burova, O. A., O. I. Zakharova, N. N. Toropova, N. A. Gladkova, and A. A. Blokhin. "The efficiency of methods for catching insects - vectors of vector-borne diseases of animals and their species composition." Agricultural Science Euro-North-East 22, no. 5 (October 27, 2021): 761–69. http://dx.doi.org/10.30766/2072-9081.2021.22.5.761-769.
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The article provides the results of study of the effectiveness of collection methods and the taxonomic identification of insects in the regions of Russia. During the research three methods of collecting insects were used: a UV trap, fly strips and a liquid gadfly trap (“death puddle”). The following blood-sucking insects play a key role in the epizootology of transmissible infections - houseflies (Muscidae), black flies (Simuliidae), mosquitoes (Culicidae), biting midges (Ceratopogonidae), gadflies (Tabanidae). There has been obtained new information on the species, territorial and temporal dynamics of the distribution of vectors of lumpy skin disease in cattle in the subjects of the Russian Federation. Data collection of the spatial and temporal spread of the disease visualized using GIS-technologies have been generated on the basis of the statistical reports. As the result of the research, it has been established that in the Nizhny Novgorod region representatives of the Psychodidae family, the percentage of which was 40.9 %, predominate in the nocturnal entomological complex. They are followed by mosquitoes (genus Culex) - 21.6 %, biting midges - 16.4 % manure flies - 7.0 % and black flies - 3.0 %, respectively. In the Saratov region, the nocturnal entomological complex was represented by manure flies (family Sphaeroceridae), black flies (family Simuliidae) and mosquitoes (genus Culex), the percentages were 56,0, 32,0 and 12,0 %, respectively. At the same time, in the Saratov region there were collected 239 times less mosquitoes than in the Nizhny Novgorod region, which was due to an increase in temperature in the conditions of the southern regions. This caused the water bodies to dry out and reduced the pool of insects whose developmental cycle is related to water. It has been established that all-year keeping of cattle in winter cow yards provides the diversity and rise in the number of insect vectors, which increases the risk of lumpy skin disease as compared to the grazing system of cattle keeping. For collection daytime insect vectors, it is recommended to use fly strips covered with rosin and mineral oil. For collection insects of the nocturnal entomocomplex, which are the main transmitters of the lumpy skin disease virus, one should use ultraviolet traps.
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Khorramnejad, Ayda, Hugo D. Perdomo, Umberto Palatini, Mariangela Bonizzoni, and Laila Gasmi. "Cross Talk between Viruses and Insect Cells Cytoskeleton." Viruses 13, no. 8 (August 20, 2021): 1658. http://dx.doi.org/10.3390/v13081658.
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Viruses are excellent manipulators of host cellular machinery, behavior, and life cycle, with the host cell cytoskeleton being a primordial viral target. Viruses infecting insects generally enter host cells through clathrin-mediated endocytosis or membrane fusion mechanisms followed by transport of the viral particles to the corresponding replication sites. After viral replication, the viral progeny egresses toward adjacent cells and reaches the different target tissues. Throughout all these steps, actin and tubulin re-arrangements are driven by viruses. The mechanisms used by viruses to manipulate the insect host cytoskeleton are well documented in the case of alphabaculoviruses infecting Lepidoptera hosts and plant viruses infecting Hemiptera vectors, but they are not well studied in case of other insect–virus systems such as arboviruses–mosquito vectors. Here, we summarize the available knowledge on how viruses manipulate the insect host cell cytoskeleton, and we emphasize the primordial role of cytoskeleton components in insect virus motility and the need to expand the study of this interaction.
10
Hommay, Gérard, Antoine Alliaume, Catherine Reinbold, and Etienne Herrbach. "Transmission of Grapevine leafroll-associated virus-1 (Ampelovirus) and Grapevine virus A (Vitivirus) by the Cottony Grape Scale, Pulvinaria vitis (Hemiptera: Coccidae)." Viruses 13, no. 10 (October 15, 2021): 2081. http://dx.doi.org/10.3390/v13102081.
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The cottony grape scale Pulvinaria vitis is a scale insect colonizing grapevine; however, its capacity as a vector of grapevine viruses is poorly known in comparison to other scale species that are vectors of viral species in the genera Ampelovirus and Vitivirus. The ability of P. vitis to transmit the ampeloviruses Grapevine leafroll-associated viruses [GLRaV]−1, −3, and −4, and the vitivirus Grapevine virus A (GVA), to healthy vine cuttings was assessed. The scale insects used originated from commercial vine plots located in Alsace, Eastern France. When nymphs sampled from leafroll-infected vineyard plants were transferred onto healthy cuttings, only one event of transmission was obtained. However, when laboratory-reared, non-viruliferous nymphs were allowed to acquire viruses under controlled conditions, both first and second instar nymphs derived from two vineyards were able to transmit GLRaV−1 and GVA. This is the first report of GLRaV−1 and GVA transmission from grapevine to grapevine by this species.
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Balaraman, Velmurugan, Barbara S. Drolet, Natasha N. Gaudreault, William C. Wilson, Jeana Owens, Dashzeveg Bold, Dustin A. Swanson, et al. "Susceptibility of Midge and Mosquito Vectors to SARS-CoV-2." Journal of Medical Entomology 58, no. 4 (March 4, 2021): 1948–51. http://dx.doi.org/10.1093/jme/tjab013.
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Abstract SARS-CoV-2 is a recently emerged, highly contagious virus and the cause of the current COVID-19 pandemic. It is a zoonotic virus, although its animal origin is not clear yet. Person-to-person transmission occurs by inhalation of infected droplets and aerosols, or by direct contact with contaminated fomites. Arthropods transmit numerous viral, parasitic, and bacterial diseases; however, the potential role of arthropods in SARS-CoV-2 transmission is not fully understood. Thus far, a few studies have demonstrated that SARS-CoV-2 replication is not supported in cells from certain insect species nor in certain species of mosquitoes after intrathoracic inoculation. In this study, we expanded the work of SARS-CoV-2 susceptibility to biting insects after ingesting a SARS-CoV-2-infected bloodmeal. Species tested included Culicoides sonorensis (Wirth & Jones) (Diptera: Ceratopogonidae) biting midges, as well as Culex tarsalis (Coquillett) and Culex quinquefasciatus (Say) mosquitoes (Diptera: Culicidae), all known biological vectors for numerous RNA viruses. Arthropods were allowed to feed on SARS-CoV-2-spiked blood and at a time point postinfection analyzed for the presence of viral RNA and infectious virus. Additionally, cell lines derived from C. sonorensis (W8a), Aedes aegypti (Linnaeus) (Diptera: Culicidae) (C6/36), Cx. quinquefasciatus (HSU), and Cx. tarsalis (CxTrR2) were tested for SARS-CoV-2 susceptibility. Our results indicate that none of the biting insects, nor the insect cell lines evaluated support SARS-CoV-2 replication, suggesting that these species are unable to be biological vectors of SARS-CoV-2.
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Xiao, Yan, Qiong Li, Wei Wang, Yumei Fu, and Feng Cui. "Regulation of RNA Interference Pathways in the Insect Vector Laodelphax striatellus by Viral Proteins of Rice Stripe Virus." Viruses 13, no. 8 (August 11, 2021): 1591. http://dx.doi.org/10.3390/v13081591.
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RNA interference (RNAi), especially the small interfering RNA (siRNA) and microRNA (miRNA) pathways, plays an important role in defending against viruses in plants and insects. However, how insect-transmitted phytoviruses regulate the RNAi-mediated antiviral response in vector insects has barely been uncovered. In this study, we explored the interaction between rice stripe virus (RSV) and the miRNA and siRNA pathways of the small brown planthopper, which is a vector insect. The transcript and protein levels of key genes in the two RNAi pathways did not change during the RSV infection process. When the expression of insect Ago1, Ago2, or Translin was silenced by the injection of double-stranded RNAs targeting these genes, viral replication was promoted with Ago2 silencing but inhibited with Translin silencing. Protein-protein binding assays showed that viral NS2 and RNA-dependent RNA polymerase interacted with insect Ago2 and Translin, respectively. When NS2 was knocked down, the transcript level of Ago2 increased and viral replication was inhibited. Therefore, viral NS2 behaved like an siRNA suppressor in vector insects. This protein-binding regulation of insect RNAi systems reflects a complicated and diverse coevolution of viruses with their vector insects.
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Fiallo-Olivé, Elvira, Li-Long Pan, Shu-Sheng Liu, and Jesús Navas-Castillo. "Transmission of Begomoviruses and Other Whitefly-Borne Viruses: Dependence on the Vector Species." Phytopathology® 110, no. 1 (January 2020): 10–17. http://dx.doi.org/10.1094/phyto-07-19-0273-fi.
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Most plant viruses require a biological vector to spread from plant to plant in nature. Among biological vectors for plant viruses, hemipteroid insects are the most common, including phloem-feeding aphids, whiteflies, mealybugs, planthoppers, and leafhoppers. A majority of the emerging diseases challenging agriculture worldwide are insect borne, with those transmitted by whiteflies (Hemiptera: Aleyrodidae) topping the list. Most damaging whitefly-transmitted viruses include begomoviruses (Geminiviridae), criniviruses (Closteroviridae), and torradoviruses (Secoviridae). Among the whitefly vectors, Bemisia tabaci, now recognized as a complex of cryptic species, is the most harmful in terms of virus transmission. Here, we review the available information on the differential transmission efficiency of begomoviruses and other whitefly-borne viruses by different species of whiteflies, including the cryptic species of the B. tabaci complex. In addition, we summarize the factors affecting transmission of viruses by whiteflies and point out some future research prospects.
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Cieniewicz, Elizabeth J., Sarah J. Pethybridge, Gregory Loeb, Keith Perry, and Marc Fuchs. "Insights Into the Ecology of Grapevine red blotch virus in a Diseased Vineyard." Phytopathology® 108, no. 1 (January 2018): 94–102. http://dx.doi.org/10.1094/phyto-07-17-0239-r.
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Limited information is available on the spread of Grapevine red blotch virus (GRBV, genus Grablovirus, family Geminiviridae) in vineyards. To investigate ecological aspects of red blotch disease spread, sticky cards to catch flying insects were placed in 2015 (April to November) and 2016 (March to November) in a vineyard study site in California where disease incidence increased by nearly 20% between 2014 and 2016. Subsets of insect species or taxa were removed from sticky card traps and individual specimens were tested for the presence of GRBV by multiplex polymerase chain reaction. GRBV was consistently detected in Spissistilus festinus (Membracidae), Colladonus reductus (Cicadellidae), Osbornellus borealis (Cicadellidae), and a Melanoliarus sp. (Cixiidae). Populations of these four candidate vectors peaked from June to September, with viruliferous S. festinus peaking from late June to early July in both years. An assessment of co-occurrence and covariation between the spatial distribution of GRBV-infected vines and viruliferous insects identified a significant association only with viruliferous S. festinus. These findings revealed the epidemiological relevance of S. festinus as a vector of GRBV in a vineyard ecosystem. Sequencing coat protein and replicase-associated protein gene fragments of GRBV isolates from newly infected vines and viruliferous vector candidates further suggested secondary spread primarily from local sources and occasionally from background sources.
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Bertola, Michela, and Franco Mutinelli. "A Systematic Review on Viruses in Mass-Reared Edible Insect Species." Viruses 13, no. 11 (November 15, 2021): 2280. http://dx.doi.org/10.3390/v13112280.
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Edible insects are expected to become an important nutrient source for animals and humans in the Western world in the near future. Only a few studies on viruses in edible insects with potential for industrial rearing have been published and concern only some edible insect species. Viral pathogens that can infect insects could be non-pathogenic, or pathogenic to the insects themselves, or to humans and animals. The objective of this systematic review is to provide an overview of the viruses detected in edible insects currently considered for use in food and/or feed in the European Union or appropriate for mass rearing, and to collect information on clinical symptoms in insects and on the vector role of insects themselves. Many different virus species have been detected in edible insect species showing promise for mass production systems. These viruses could be a risk for mass insect rearing systems causing acute high mortality, a drastic decline in growth in juvenile stages and in the reproductive performance of adults. Furthermore, some viruses could pose a risk to human and animal health where insects are used for food and feed.
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Isah, U., and M. A. Ahmad. "Microorganisms as bioinsecticides; short review." Bayero Journal of Pure and Applied Sciences 12, no. 1 (April 15, 2020): 274–79. http://dx.doi.org/10.4314/bajopas.v12i1.42s.
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Hundred thousand tons of chemical insecticides are used annually in Nigeria to combat insect disease vectors especially agricultural pests, but this sort of vector control method is gradually being substituted due to their environmental effects on non-target beneficial insects especially vertebrates through contamination of food and water. To counteract this contamination, attention, efforts and researches were directed to the use of biological control agents including insect pathogens. As a result, the use of bio insecticide, as a component of integrated pest management (IPM), has been gaining acceptance over the world. Microbial pathogens comprise of organisms which cause disease, these organisms are disseminated in the pest population in large quantity in a manner similar to application of chemical pesticides. Insects like other organisms are susceptible to a variety of diseases caused by different groups of microorganisms including virus, bacteria, fungi, protozoa and nematodes. Microbial pathogens of insects are intensively investigated to develop environment friendly pest management strategies in agriculture. Microbial insecticides represent today the best alternative to chemical insecticides in controlling insect pests, they are safe for non-target species and human health are believed to show low persistence in the environment. This short review indicates that microbial insecticides are the safe alternative way possessing all the requirements to replace chemical insecticides hence, they can be utilized in pest management and control.
Keywords: Bio-insecticides, Microorganisms, biological control, pest, chemical insecticides
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Kenney, Jaimie R., Marie-Eve Grandmont, and Kerry E. Mauck. "Priming Melon Defenses with Acibenzolar-S-methyl Attenuates Infections by Phylogenetically Distinct Viruses and Diminishes Vector Preferences for Infected Hosts." Viruses 12, no. 3 (February 26, 2020): 257. http://dx.doi.org/10.3390/v12030257.
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Plant virus management is mostly achieved through control of insect vectors using insecticides. However, insecticides are only marginally effective for preventing virus transmission. Furthermore, it is well established that symptoms of virus infections often encourage vector visitation to infected hosts, which exacerbates secondary spread. Plant defense elicitors, phytohormone analogs that prime the plant immune system against attack, may be a viable approach for virus control that complements insecticide use by disrupting pathologies that attract vectors. To explore this, we tested the effect of a commercial plant elicitor, acibenzolar-S-methyl (ASM), on infection rates, virus titers, and symptom development in melon plants inoculated with one of two virus species, Cucumber mosaic virus (CMV) and Cucurbit yellow stunting disorder virus (CYSDV). We also conducted behavioral assays to assess the effect of ASM treatment and virus inoculation on vector behavior. For both pathogens, ASM treatment reduced symptom severity and delayed disease progression. For CYSDV, this resulted in the attenuation of symptoms that encourage vector visitation and virion uptake. We did observe slight trade-offs in growth vs. defense following ASM treatment, but these effects did not translate into reduced yields or plant performance in the field. Our results suggest that immunity priming may be a valuable tool for improving management of insect-transmitted plant viruses.
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Sudiarta, I. Putu, Gusti Ngurah Alit Susanta Wirya, Dewa Gede Wiryangga Selangga, and Made Getas Pudak Wangi. "Detection of Strawberry vein banding virus (SVBV) and Identification of Viruliferous Insects Associated with Strawberry Plants (Fragaria sp.) in Bali." Jurnal Perlindungan Tanaman Indonesia 25, no. 2 (December 5, 2021): 121. http://dx.doi.org/10.22146/jpti.57714.
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Plant pests and diseases cause decreases in strawberry yield in Bali, including plant viruses. Strawberry vein banding virus (SVBV) is a virus that infects strawberry plants and is transmitted by insects. However, studies about this disease in Bali are still limited. This study aimed to detect SVBV within insect bodies and determine the insect species of its vector. Methods used included (1) sampling; (2) detection of SVBV from insect bodies using primers (SVBV F/SVBV R) and PCR; and (3) molecular identification of viruliferous insect with primers (LCO 1490 F/HCO 2198 R) using PCR and sequencing analysis. This study successfully detected SVBV DNA in an insect from three insect families associated with strawberry plants in Pancasari Village, Buleleng Regency, Bali. Results from the nucleotide sequences analysis in SVBV viruliferous insects indicate that the insect was Myzus persicae.
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BALFAS, RODIAH, SUPRIADI SUPRIADI, T. L. MARDININGSIH, and ENDANG SUGANDI. "PENYEBAB DAN SERANGGA VEKTOR PENYAKIT KERITING PADA TANAMAN LADA." Jurnal Penelitian Tanaman Industri 8, no. 1 (July 15, 2020): 7. http://dx.doi.org/10.21082/jlittri.v8n1.2002.7-11.
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The cause of stunting disease of black pepper (Piper nigrum) in Indonesia has not yet been confirmed cither due o a virus or mycoplaam. However, similar disease found on black pepper plants in the Southeast Asia is caused by Piper yellow mottle virus (PYMV) which is transmitted by Planococcus citri. This expeiments was aimed o examine the cause of the stunting disease and its insect vectors. The expeiment were conducted from October 1998 to May 2001. Diseased vegetative materials of black pepper plants showing stunting disease were collected from Sukamulya, Sukabumi. The potential insect vectors were collected from black pepper plants in Bogor, IP Sukamulya (Sukabumi), Lampung and Bangka. The insects were fed on the diseased plants obtained from Lampung, Bogor and IP Sukamulya, then transferred o the healthy plants. The healthy plants were produced rom true seeds and cutings oiginated rom Bogor and IP Sukamulya. The tested plants were incubated at the green house and examined for disease development. Diseased leaf samples collected rom black pepper plant rom IP. Sukamulya and the transmitted plants were sent to the University of Minnesota, USA for Ihe virus (PYMV) by using ISEM (immunosorbent electron microscope). The result snowed that the leaves samples rom IP. Sukamulya were infected by PYMV. The morphology and size of the virus were similar to those caused stunting disease in the South East Asia. Potential insects vectors found on the diseased black <br /><br />pepper plants were two mealybugs, P. minor and Ferrisia virgata (Hemiptera; Coccoidca: PaaidbcoccidaeX aa well as an aphid Toxoptera aurantii (Hemiptera: Aphidoidea: Aphididae). The first tial on disease transmission by using P. minor, previously reared on the potato tubers, showed one out of ten tested plants produced disease sympom. The subsequent trial using P minor, bred on healthy black pepper seedlings, showed thee out often tested plants developed disease symptoms. None of the aphid transmitted plants developed Ihe disease. This study confirmed thai PYMV b the cause of stunting disease on black pepper in IP Sukamulya and Lampung and P. minor as the insect vecor of the disease.<br /><br />
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Braxton, Cassandra L., Shelby H. Puckett, Steven B. Mizel, and Douglas S. Lyles. "Protection against Lethal Vaccinia Virus Challenge by Using an Attenuated Matrix Protein Mutant Vesicular Stomatitis Virus Vaccine Vector Expressing Poxvirus Antigens." Journal of Virology 84, no. 7 (January 20, 2010): 3552–61. http://dx.doi.org/10.1128/jvi.01572-09.
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ABSTRACT Recombinant vesicular stomatitis viruses (VSV) are excellent candidate vectors for vaccination against human diseases. The neurovirulence of VSV in animal models requires the attenuation of the virus for use in humans. Previous efforts have focused on attenuating virus replication. Studies presented here test an alternative approach for attenuation that uses a matrix (M) protein mutant (rM51R) VSV as a vaccine vector against respiratory infection. This mutant is attenuated for viral virulence by its inability to suppress the innate immune response. The ability of rM51R VSV vectors to protect against lethal respiratory challenge was tested using a vaccinia virus intranasal challenge model. Mice immunized intranasally with rM51R vectors expressing vaccinia virus antigens B5R and L1R were protected against lethal vaccinia virus challenge. A single immunization with the vectors provided protection against vaccinia virus-induced mortality; however, a prime-boost strategy reduced the severity of the vaccinia virus-induced disease progression. Antibody titers measured after the prime and boost were low despite complete protection against lethal challenge. However, immunized animals had higher antibody titers during the challenge, suggesting that memory B-cell responses may be important for the protection. Depletion experiments demonstrated that B cells but not CD8 T cells were involved in the protection mediated by rM51R vaccine vectors that express B5R and L1R. These results demonstrate the potential of M protein mutant VSVs as candidate vaccine vectors against human diseases.
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Fila, Mateusz, and Grzegorz Woźniakowski. "African swine fever virus – the possible role of flies and other insects in virus transmission." Journal of Veterinary Research 64, no. 1 (January 20, 2020): 1–7. http://dx.doi.org/10.2478/jvetres-2020-0001.
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AbstractAfrican swine fever (ASF) is an acute viral haemorrhagic disease of pigs and wild boars. It presents a serious threat to pig production worldwide, and since 2007, ASF outbreaks have been recorded in the Caucasus, Eastern Europe, and the Baltic States. In 2014, the disease was detected in Poland. ASF is on the list of notifiable diseases of the World Organisation for Animal Health (OIE). Due to the lack of an available vaccine and treatment, the countermeasures against the disease consist in early detection of the virus in the pig population and control of its spread through the elimination of herds affected by disease outbreaks. Knowledge of the potential vectors of the virus and its persistence in the environment is crucial to prevent further disease spread and to understand the new epidemiology for how it compares to the previous experience in Spain gathered in the 1970s and 1980s.
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Ghosh, Saptarshi, and Murad Ghanim. "Factors Determining Transmission of Persistent Viruses by Bemisia tabaci and Emergence of New Virus–Vector Relationships." Viruses 13, no. 9 (September 11, 2021): 1808. http://dx.doi.org/10.3390/v13091808.
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Many plant viruses depend on insect vectors for their transmission and dissemination. The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is one of the most important virus vectors, transmitting more than four hundred virus species, the majority belonging to begomoviruses (Geminiviridae), with their ssDNA genomes. Begomoviruses are transmitted by B. tabaci in a persistent, circulative manner, during which the virus breaches barriers in the digestive, hemolymph, and salivary systems, and interacts with insect proteins along the transmission pathway. These interactions and the tissue tropism in the vector body determine the efficiency and specificity of the transmission. This review describes the mechanisms involved in circulative begomovirus transmission by B. tabaci, focusing on the most studied virus in this regard, namely the tomato yellow leaf curl virus (TYLCV) and its closely related isolates. Additionally, the review aims at drawing attention to the recent knowhow of unorthodox virus—B. tabaci interactions. The recent knowledge of whitefly-mediated transmission of two recombinant poleroviruses (Luteoviridae), a virus group with an ssRNA genome and known to be strictly transmitted with aphids, is discussed with its broader context in the emergence of new whitefly-driven virus diseases.
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Csizinszky, A. A., D. J. Schuster, and J. B. Kring. "EFFECT OF MULCH COLOR ON TOMATO YIELDS AND ON INSECT VECTORS." HortScience 25, no. 9 (September 1990): 1131e—1131. http://dx.doi.org/10.21273/hortsci.25.9.1131e.
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Field studies were conducted for three seasons, Fall 1988, and Spring and Fall of 1989 on the effect of six mulch colors: blue, orange, red, aluminum, white or black (fall or spring), and yellow on fruit yields and on insect vectors of `Sunny' tomato, In Fall 1988, in a single harvest, fruit size was greater and total marketable yields were higher with blue than with aluminum and yellow mulches. In Spring 1989 early yields of large (> 70 mm) and marketable fruit were higher with aluminum and red than with yellow and blue mulches. In Fall 1989 early yield of large fruit was higher with white than with yellow mulch. Early marketable yields were highest with white and aluminum mulches. Total yields of large fruits were highest with orange and blue mulches but marketable yields were similar with all six mulch colors. The fewest number of aphids, thrips and whiteflies were trapped on aluminum mulch. Blue mulch attracted the largest number of aphids and thrips. Red mulch attracted whiteflies. The three insects are important vectors of several virus diseases.
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Qadafi, David Mohamad, Poedji Hastutiek, Lilik Maslachah, Endang Suprihati, and Muhammad Hambal. "Repellent Effectiveness of Permot Leaf Ethanol Extract (Passiflora Foetida Linn.) against Aedes Aegypti Adult Mosquitoes." Journal of Parasite Science 5, no. 1 (September 15, 2021): 25. http://dx.doi.org/10.20473/jops.v5i1.29962.
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Indonesia is one of the largest tropical countries in the world and various diseases can arise in the tropics which are caused by animals as vectors. An example of a vector that can carry diseases is a mosquito. Mosquitoes are insects that live side by side with humans buy act as vectors of disease. Mosquito Aedes aegypti is a type of mosquito that can carry the virus that causes Dengue Hemorrhagic Fever. This research was conducted to determine the effectiveness of Permot leaf ethanol extract (Passiflora foetida L.) as an alternative repellent against adult mosquitoes Aedes aegypti. This research was conducted from October to December 2020 and used Permot leaf ethanol extract consisting of 3 cream concentrations, namely 2.5%, 5%, 7.5%, negative control using cream without permot leaf ethanol extract and positive control using mosquito cream. The data of this study were tested using one way ANOVA to find the effectiveness rate and comparations of the each Permot leaf repellent extract. This study proven that the permot leaf ethanol extract is effective as a repellent for Aedes aegypti mosquitoes.
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Ravelonandro, M. "Gene-for-gene interactions are required for disease resistance mediated by virus transgene." Plant Protection Science 38, SI 1 - 6th Conf EFPP 2002 (January 1, 2002): 177–79. http://dx.doi.org/10.17221/10349-pps.
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Plant viruses cause severe damage and significant economic losses to agriculture. Control of virus usually consist of<br />the elimination of virus vectors (insects, nematodes, fungi, etc), improvement of the sanitary status of the propagation<br />material, the use of resistance sources in breeding programs. The application of the pathogen-derived resistance strategy<br />has opened new avenues to protect plants against viruses. Two molecular mechanisms seem to underlie the engineered<br />protection, the virus transgene-derived protein and the transgene-RNA interference. A few examples that support the<br />efficiencies of these two molecular mechanisms are reviewed here and discussed in light of the potential use of virusresistant<br />transgenic plants in agriculture.
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Garzo, Elisa, Aránzazu Moreno, María Plaza, and Alberto Fereres. "Feeding Behavior and Virus-transmission Ability of Insect Vectors Exposed to Systemic Insecticides." Plants 9, no. 7 (July 15, 2020): 895. http://dx.doi.org/10.3390/plants9070895.
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The majority of plant viruses depend on Hemipteran vectors for their survival and spread. Effective management of these insect vectors is crucial to minimize the spread of vector-borne diseases, and to reduce crop damage. The aim of the present study was to evaluate the effect of various systemic insecticides on the feeding behavior of Bemisia tabaci and Myzus persicae, as well as their ability to interfere with the transmission of circulative viruses. The obtained results indicated that some systemic insecticides have antifeeding properties that disrupt virus transmission by their insect vectors. We found that some of the tested insecticides significantly reduced phloem contact and sap ingestion by aphids and whiteflies, activities that are closely linked to the transmission of phloem-limited viruses. These systemic insecticides may play an important role in reducing the primary and secondary spread of tomato yellow leaf curl virus (TYLCV) and turnip yellows virus (TuYV), transmitted by B. tabaci and M. persicae, respectively.
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Krebs, Bethany L., Tavis K. Anderson, Tony L. Goldberg, Gabriel L. Hamer, Uriel D. Kitron, Christina M. Newman, Marilyn O. Ruiz, Edward D. Walker, and Jeffrey D. Brawn. "Host group formation decreases exposure to vector-borne disease: a field experiment in a ‘hotspot’ of West Nile virus transmission." Proceedings of the Royal Society B: Biological Sciences 281, no. 1796 (December 7, 2014): 20141586. http://dx.doi.org/10.1098/rspb.2014.1586.
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Animals can decrease their individual risk of predation by forming groups. The encounter-dilution hypothesis extends the potential benefits of gregariousness to biting insects and vector-borne disease by predicting that the per capita number of insect bites should decrease within larger host groups. Although vector-borne diseases are common and can exert strong selective pressures on hosts, there have been few tests of the encounter-dilution effect in natural systems. We conducted an experimental test of the encounter-dilution hypothesis using the American robin ( Turdus migratorius ), a common host species for the West Nile virus (WNV), a mosquito-borne pathogen. By using sentinel hosts (house sparrows, Passer domesticus ) caged in naturally occurring communal roosts in the suburbs of Chicago, we assessed sentinel host risk of WNV exposure inside and outside of roosts. We also estimated per capita host exposure to infected vectors inside roosts and outside of roosts. Sentinel birds caged inside roosts seroconverted to WNV more slowly than those outside of roosts, suggesting that social groups decrease per capita exposure to infected mosquitoes. These results therefore support the encounter-dilution hypothesis in a vector-borne disease system. Our results suggest that disease-related selective pressures on sociality may depend on the mode of disease transmission.
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Yu, Qian, Pengfei Chang, Xiaoxuan Liu, Peng Lü, Qi Tang, Zhongjian Guo, Jianming Qiu, Keping Chen, and Qin Yao. "Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System." Viruses 13, no. 4 (April 18, 2021): 704. http://dx.doi.org/10.3390/v13040704.
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Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. To establish a more efficient production system, Bombyx mori pupae were used as a new platform and infected with recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV). The production of a chimeric recombinant adeno-associated virus (rAAV) serotype 2/human bocavirus type-1 (HBoV1) vector was used to evaluate the efficiency of this new baculovirus expression vector (BEV)–insect expression system. For this purpose, we constructed two recombinant BmNPVs, which were named rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP. The yields of rAAV2/HBoV1 derived from the rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP co-infected BmN cells exceeded 2 × 104 vector genomes (VG) per cell. The rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP can express stably for at least five passages. Significantly, rAAV2/HBoV1 could be efficiently generated from BmNPV-infected silkworm larvae and pupae at average yields of 2.52 × 1012 VG/larva and 4.6 × 1012 VG/pupa, respectively. However, the vectors produced from the larvae and pupae had a high percentage of empty particles, which suggests that further optimization is required for this platform in the future. Our work shows that silkworm pupae, as an efficient bioreactor, have great potential for application in the production of gene therapy vectors.
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Eberwine, John W., and Edward S. Hagood. "Effect of Johnsongrass (Sorghum halepense) Control on the Severity of Virus Diseases of Corn (Zea mays)." Weed Technology 9, no. 1 (March 1995): 73–79. http://dx.doi.org/10.1017/s0890037x00022983.
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Field experiments were conducted to evaluate the hypothesis that johnsongrass control in corn causes increased maize dwarf mosaic virus (MDMV) and maize chlorotic dwarf virus (MCDV) disease severity because of increased movement of insect vectors from dying johnsongrass to the corn crop. Johnsongrass control treatments included 1) broadcast POST nicosulfuron, 2) directed POST imazethapyr, 3) mechanical control, and 4) no treatment. Disease severity in both a virus-susceptible and a virus-tolerant corn hybrid was evaluated. With the virus-susceptible hybrid, greater disease severity was observed where johnsongrass was controlled in the experimental area than where johnsongrass was not controlled. Increases in disease severity were independent of the method of johnsongrass control. Corollary studies conducted on the same site verified a double infection of corn with MDMV and MCDV and documented movement of blackfaced leafhoppers, the insect vector of MCDV, subsequent to treatment of johnsongrass.
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Krishnan, Jayalakshmi. "Monitoring of Insecticide Resistance and Exploring the Presence of Virus in Field Populations of Culex gelidus at Thiruvarur District of Tamil Nadu, India." Journal of Communicable Diseases 53, no. 04 (December 31, 2021): 76–83. http://dx.doi.org/10.24321/0019.5138.202177.
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Introduction: The introduction of potent synthetic insecticides into public health programmes has since beginning posed the challenge of development of resistance among the insect vectors against the insecticides. Culex (Cx.) gelidus is one of the vectors of JE which is abundantly found in southern India. Its breeding habitats are similar to the vishnui subgroup of Culex mosquitoes, the major vectors for transmission of JE. The present study was aimed to assess the susceptibility status of adult Culex (Cx.) gelidus, to insecticides, namely DDT (Dichlorodiphenyltrichloroethane), Deltamethrin, and Malathion. Method: The field-collected mosquito larvae from ten villages of Thiruvarur district from December 2018 to May 2019 were reared in the laboratory until F1 generation and the emerged adults identified as Cx. gelidus were exposed to insecticide-impregnated papers supplied through World Health Organization (WHO). The adult susceptibility tests were carried out as per the protocol of WHO. Further, an attempt was made to check the presence of JE virus in Cx. gelidus and the virus detection was done by RT-PCR. Results: The results indicated that the adult Cx. gelidus populations were susceptible to DDT, whereas they were resistant to Malathion and Deltamethrin. The possible reason of DDT susceptibility may be that DDT has not been used in Tamil Nadu since the year 1980 (about 40 years). Conclusion: JE virus was not detected in the tested mosquitoes. The study suggests that insecticide resistance monitoring from time to time is required to facilitate vector control programmes in focusing on appropriate vector control measures.
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Jones, Roger A. C., Murray Sharman, Piotr Trębicki, Solomon Maina, and Benjamin S. Congdon. "Virus Diseases of Cereal and Oilseed Crops in Australia: Current Position and Future Challenges." Viruses 13, no. 10 (October 12, 2021): 2051. http://dx.doi.org/10.3390/v13102051.
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This review summarizes research on virus diseases of cereals and oilseeds in Australia since the 1950s. All viruses known to infect the diverse range of cereal and oilseed crops grown in the continent’s temperate, Mediterranean, subtropical and tropical cropping regions are included. Viruses that occur commonly and have potential to cause the greatest seed yield and quality losses are described in detail, focusing on their biology, epidemiology and management. These are: barley yellow dwarf virus, cereal yellow dwarf virus and wheat streak mosaic virus in wheat, barley, oats, triticale and rye; Johnsongrass mosaic virus in sorghum, maize, sweet corn and pearl millet; turnip yellows virus and turnip mosaic virus in canola and Indian mustard; tobacco streak virus in sunflower; and cotton bunchy top virus in cotton. The currently less important viruses covered number nine infecting nine cereal crops and 14 infecting eight oilseed crops (none recorded for rice or linseed). Brief background information on the scope of the Australian cereal and oilseed industries, virus epidemiology and management and yield loss quantification is provided. Major future threats to managing virus diseases effectively include damaging viruses and virus vector species spreading from elsewhere, the increasing spectrum of insecticide resistance in insect and mite vectors, resistance-breaking virus strains, changes in epidemiology, virus and vectors impacts arising from climate instability and extreme weather events, and insufficient industry awareness of virus diseases. The pressing need for more resources to focus on addressing these threats is emphasized and recommendations over future research priorities provided.
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Jones, Roger A. C., Murray Sharman, Piotr Trębicki, Solomon Maina, and Benjamin S. Congdon. "Virus Diseases of Cereal and Oilseed Crops in Australia: Current Position and Future Challenges." Viruses 13, no. 10 (October 12, 2021): 2051. http://dx.doi.org/10.3390/v13102051.
Full textAbstract:
This review summarizes research on virus diseases of cereals and oilseeds in Australia since the 1950s. All viruses known to infect the diverse range of cereal and oilseed crops grown in the continent’s temperate, Mediterranean, subtropical and tropical cropping regions are included. Viruses that occur commonly and have potential to cause the greatest seed yield and quality losses are described in detail, focusing on their biology, epidemiology and management. These are: barley yellow dwarf virus, cereal yellow dwarf virus and wheat streak mosaic virus in wheat, barley, oats, triticale and rye; Johnsongrass mosaic virus in sorghum, maize, sweet corn and pearl millet; turnip yellows virus and turnip mosaic virus in canola and Indian mustard; tobacco streak virus in sunflower; and cotton bunchy top virus in cotton. The currently less important viruses covered number nine infecting nine cereal crops and 14 infecting eight oilseed crops (none recorded for rice or linseed). Brief background information on the scope of the Australian cereal and oilseed industries, virus epidemiology and management and yield loss quantification is provided. Major future threats to managing virus diseases effectively include damaging viruses and virus vector species spreading from elsewhere, the increasing spectrum of insecticide resistance in insect and mite vectors, resistance-breaking virus strains, changes in epidemiology, virus and vectors impacts arising from climate instability and extreme weather events, and insufficient industry awareness of virus diseases. The pressing need for more resources to focus on addressing these threats is emphasized and recommendations over future research priorities provided.
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Tan, Lu, Yiwen Zhang, Xingxing Wang, and Dal Young Kim. "A Productive Expression Platform Derived from Host-Restricted Eilat Virus: Its Extensive Validation and Novel Strategy." Viruses 13, no. 4 (April 11, 2021): 660. http://dx.doi.org/10.3390/v13040660.
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Most alphaviruses are transmitted by mosquitoes and infect a wide range of insects and vertebrates. However, Eilat virus (EILV) is defective for infecting vertebrate cells at multiple levels of the viral life cycle. This host-restriction property renders EILV an attractive expression platform since it is not infectious for vertebrates and therefore provides a highly advantageous safety profile. Here, we investigated the feasibility of versatile EILV-based expression vectors. By replacing the structural genes of EILV with those of other alphaviruses, we generated seven different chimeras. These chimeras were readily rescued in the original mosquito cells and were able to reach high titers, suggesting that EILV is capable of packaging the structural proteins of different lineages. We also explored the ability of EILV to express authentic antigens via double subgenomic (SG) RNA vectors. Four foreign genetic materials of varied length were introduced into the EILV genome, and the expressed heterologous genetic materials were readily detected in the infected cells. By inserting an additional SG promoter into the chimera genome containing the structural genes of Chikungunya virus (CHIKV), we developed a bivalent vaccine candidate against CHIKV and Zika virus. These data demonstrate the outstanding compatibility of the EILV genome. The produced recombinants can be applied to vaccine and diagnostic tool development, but more investigations are required.
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Modha, Sejal, Joseph Hughes, Giovanni Bianco, Heather M. Ferguson, Barbara Helm, Lily Tong, Gavin S. Wilkie, Alain Kohl, and Esther Schnettler. "Metaviromics Reveals Unknown Viral Diversity in the Biting Midge Culicoides impunctatus." Viruses 11, no. 9 (September 17, 2019): 865. http://dx.doi.org/10.3390/v11090865.
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Biting midges (Culicoides species) are vectors of arboviruses and were responsible for the emergence and spread of Schmallenberg virus (SBV) in Europe in 2011 and are likely to be involved in the emergence of other arboviruses in Europe. Improved surveillance and better understanding of risks require a better understanding of the circulating viral diversity in these biting insects. In this study, we expand the sequence space of RNA viruses by identifying a number of novel RNA viruses from Culicoides impunctatus (biting midge) using a meta-transcriptomic approach. A novel metaviromic pipeline called MetaViC was developed specifically to identify novel virus sequence signatures from high throughput sequencing (HTS) datasets in the absence of a known host genome. MetaViC is a protein centric pipeline that looks for specific protein signatures in the reads and contigs generated as part of the pipeline. Several novel viruses, including an alphanodavirus with both segments, a novel relative of the Hubei sobemo-like virus 49, two rhabdo-like viruses and a chuvirus, were identified in the Scottish midge samples. The newly identified viruses were found to be phylogenetically distinct to those previous known. These findings expand our current knowledge of viral diversity in arthropods and especially in these understudied disease vectors.
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Schmahl, Günter, Volker Walldorf, Sven Klimpel, Saleh Al-Quraishy, and Heinz Mehlhorn. "Efficacy of Oxyfly™ on Culicoides species—the vectors of Bluetongue virus—and other insects." Parasitology Research 103, no. 5 (July 22, 2008): 1101–3. http://dx.doi.org/10.1007/s00436-008-1098-x.
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Díaz, Beatriz M., Ricardo Biurrún, Aránzazu Moreno, Miguel Nebreda, and Alberto Fereres. "Impact of Ultraviolet-blocking Plastic Films on Insect Vectors of Virus Diseases Infesting Crisp Lettuce." HortScience 41, no. 3 (June 2006): 711–16. http://dx.doi.org/10.21273/hortsci.41.3.711.
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Ultraviolet (UV)-absorbing plastic films are being used as a photoselective barrier to control insect vectors and associated virus diseases in different horticultural crops. A 2-year experiment was carried out in northeastern Spain (Navarra) to evaluate the impact of a UV-blocking film (AD-IR AV) on the population density of insect pests and the spread of insect-transmitted virus diseases associated with head lettuce [Lactuca sativa (L.)]. Results showed that the UV-absorbing plastic film did not loose its ability to filter UV radiation after three lettuce crop cycles (14 months). The UV-absorbing plastic film was effective in reducing the abundance and in delaying the colonization of lettuce by aphids [Macrosiphum euphorbiae (Thomas) and Acyrthosiphum lactucae (Passerini)]. A significant increase in the percentage of marketable plants was achieved under UV-absorbing films due to a reduction in the number of plants infested by aphids and by insect-transmitted virus diseases (mainly potyviruses). Also the UV-absorbing plastic films were effective in reducing the population density of Frankliniella occidentalis (Pergande) and the spread of tomato spotted wilt virus (TSWV) as well as the population density of the lepidopteran pest, Autographa gamma (L.), a common pest of lettuce in Spain. However, no effective control of the greenhouse whitefly Trialeurodes vaporariorum (Westwood) was achieved. The results showed that UV-absorbing plastic films are a very promising tool to protect greenhouse lettuce from the main pests and insect-transmitted virus diseases occurring in northeastern Spain.
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Antignus, Yeheskel, Netta Mor, Rachel Ben Joseph, Moshe Lapidot, and Shlomo Cohen. "Ultraviolet-Absorbing Plastic Sheets Protect Crops from Insect Pests and from Virus Diseases Vectored by Insects." Environmental Entomology 25, no. 5 (October 1, 1996): 919–24. http://dx.doi.org/10.1093/ee/25.5.919.
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Sumner-Jones, Stephanie G., Deborah R. Gill, and Stephen C. Hyde. "Lack of Repeat Transduction by Recombinant Adeno-Associated Virus Type 5/5 Vectors in the Mouse Airway." Journal of Virology 81, no. 22 (September 12, 2007): 12360–67. http://dx.doi.org/10.1128/jvi.01010-07.
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ABSTRACT While recombinant adeno-associated virus (rAAV) vectors promote long-term transgene expression in the lungs and other organs, the goal of correcting chronic inherited lung diseases such as cystic fibrosis with this type of viral gene transfer vector is limited by the requirement of achieving stable potent transgene expression, potentially requiring vector readministration. Here we evaluated the abilities of rAAV type 5/5 (rAAV5/5) vectors based on the genome and capsid of AAV5 to efficiently transduce the lungs and nasal epithelium of mice after repeated administration. Transduction efficiency as judged by reporter gene expression was markedly reduced on a second rAAV5/5 administration and effectively abolished on a third. Varying the period between administrations from 8 to 36 weeks did not allow efficient repeated administration. A rapid rise in anti-AAV5 antibodies was noted after rAAV5/5 vector administration that was sustained for the entire period of investigation (in some cases exceeding 9 months). Furthermore, this antibody response and subsequent failure to repeatedly administer the vector were not rescued by the in vivo expression of CTLA4Ig from an rAAV5/5 vector. These results suggest that without the development of an effective and clinically acceptable immunosuppression strategy, treatments for chronic diseases that require repeated administration of rAAV5/5 vectors will be unsuccessful.
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Nakai, Hiroyuki, Sally Fuess, Theresa A. Storm, Shin-ichi Muramatsu, Yuko Nara, and Mark A. Kay. "Unrestricted Hepatocyte Transduction with Adeno-Associated Virus Serotype 8 Vectors in Mice." Journal of Virology 79, no. 1 (January 1, 2005): 214–24. http://dx.doi.org/10.1128/jvi.79.1.214-224.2005.
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ABSTRACT Recombinant adeno-associated virus (rAAV) vectors can mediate long-term stable transduction in various target tissues. However, with rAAV serotype 2 (rAAV2) vectors, liver transduction is confined to only a small portion of hepatocytes even after administration of extremely high vector doses. In order to investigate whether rAAV vectors of other serotypes exhibit similar restricted liver transduction, we performed a dose-response study by injecting mice with β-galactosidase-expressing rAAV1 and rAAV8 vectors via the portal vein. The rAAV1 vector showed a blunted dose-response similar to that of rAAV2 at high doses, while the rAAV8 vector dose-response remained unchanged at any dose and ultimately could transduce all the hepatocytes at a dose of 7.2 × 1012 vector genomes/mouse without toxicity. This indicates that all hepatocytes have the ability to process incoming single-stranded vector genomes into duplex DNA. A single tail vein injection of the rAAV8 vector was as efficient as portal vein injection at any dose. In addition, intravascular administration of the rAAV8 vector at a high dose transduced all the skeletal muscles throughout the body, including the diaphragm, the entire cardiac muscle, and substantial numbers of cells in the pancreas, smooth muscles, and brain. Thus, rAAV8 is a robust vector for gene transfer to the liver and provides a promising research tool for delivering genes to various target organs. In addition, the rAAV8 vector may offer a potential therapeutic agent for various diseases affecting nonhepatic tissues, but great caution is required for vector spillover and tight control of tissue-specific gene expression.
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Brown, Charles R., Mary Bomberger Brown, Amy T. Moore, and Nicholas Komar. "Bird Movement Predicts Buggy Creek Virus Infection in Insect Vectors." Vector-Borne and Zoonotic Diseases 7, no. 3 (September 2007): 304–14. http://dx.doi.org/10.1089/vbz.2006.0646.
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Moore, Amy T., and Charles R. Brown. "Dispersing hemipteran vectors have reduced arbovirus prevalence." Biology Letters 10, no. 4 (April 2014): 20140117. http://dx.doi.org/10.1098/rsbl.2014.0117.
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A challenge in managing vector-borne zoonotic diseases in human and wildlife populations is predicting where epidemics or epizootics are likely to occur, and this requires knowing in part the likelihood of infected insect vectors dispersing pathogens from existing infection foci to novel areas. We measured prevalence of an arbovirus, Buggy Creek virus, in dispersing and resident individuals of its exclusive vector, the ectoparasitic swallow bug ( Oeciacus vicarius ), that occupies cliff swallow ( Petrochelidon pyrrhonota ) colonies in western Nebraska. Bugs colonizing new colony sites and immigrating into established colonies by clinging to the swallows’ legs and feet had significantly lower virus prevalence than bugs in established colonies and those that were clustering in established colonies before dispersing. The reduced likelihood of infected bugs dispersing to new colony sites indicates that even heavily infected sites may not always export virus to nearby foci at a high rate. Infected arthropods should not be assumed to exhibit the same dispersal or movement behaviour as uninfected individuals, and these differences in dispersal should perhaps be considered in the epidemiology of vector-borne pathogens such as arboviruses.
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Kobayashi, Masanori, Akihiro Iida, Yasuji Ueda, and Mamoru Hasegawa. "Pseudotyped Lentivirus Vectors Derived from Simian Immunodeficiency Virus SIVagm with Envelope Glycoproteins from Paramyxovirus." Journal of Virology 77, no. 4 (February 15, 2003): 2607–14. http://dx.doi.org/10.1128/jvi.77.4.2607-2614.2003.
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ABSTRACT We describe the development of novel lentivirus vectors based on simian immunodeficiency virus from African green monkey (SIVagm) pseudotyped with Sendai virus (SeV) envelope glycoproteins. SeV fusion (F) and hemagglutinin-neuraminidase (HN) proteins were successfully incorporated into the SIVagm-based vector by truncation of the cytoplasmic tail of the F protein and by addition of the cytoplasmic tail of SIVagm transmembrane envelope protein to the N terminus of the HN protein. As with the vesicular stomatitis virus G glycoprotein-pseudotyped vector, the mutant SeV F- and HN-pseudotyped SIVagm vector was able to transduce various types of animal and human cell lines. Furthermore, the vector was able to transduce an enhanced green fluorescent protein reporter gene into polarized epithelial cells of rat trachea from the apical and basolateral sides. Therefore, SeV F- and HN-pseudotyped SIVagm vectors have considerable potential for effective use in gene therapy for various therapies, including respiratory diseases.
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van Munster, Manuella. "Impact of Abiotic Stresses on Plant Virus Transmission by Aphids." Viruses 12, no. 2 (February 14, 2020): 216. http://dx.doi.org/10.3390/v12020216.
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Plants regularly encounter abiotic constraints, and plant response to stress has been a focus of research for decades. Given increasing global temperatures and elevated atmospheric CO2 levels and the occurrence of water stress episodes driven by climate change, plant biochemistry, in particular, plant defence responses, may be altered significantly. Environmental factors also have a wider impact, shaping viral transmission processes that rely on a complex set of interactions between, at least, the pathogen, the vector, and the host plant. This review considers how abiotic stresses influence the transmission and spread of plant viruses by aphid vectors, mainly through changes in host physiology status, and summarizes the latest findings in this research field. The direct effects of climate change and severe weather events that impact the feeding behaviour of insect vectors as well as the major traits (e.g., within-host accumulation, disease severity and transmission) of viral plant pathogens are discussed. Finally, the intrinsic capacity of viruses to react to environmental cues in planta and how this may influence viral transmission efficiency is summarized. The clear interaction between biotic (virus) and abiotic stresses is a risk that must be accounted for when modelling virus epidemiology under scenarios of climate change.
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Xiao, Weidong, Scott C. Berta, Min Min Lu, A. David Moscioni, John Tazelaar, and James M. Wilson. "Adeno-Associated Virus as a Vector for Liver-Directed Gene Therapy." Journal of Virology 72, no. 12 (December 1, 1998): 10222–26. http://dx.doi.org/10.1128/jvi.72.12.10222-10226.1998.
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ABSTRACT Factors relevant to the successful application of adeno-associated virus (AAV) vectors for liver-directed gene therapy were evaluated. Vectors with different promoters driving expression of human α-1-antitrypsin (α-1AT) were injected into the portal circulation of immunodeficient mice. α-1AT expression was stable but dependent on the promoter. Southern analysis of liver DNA revealed approximately 0.1 to 2.0 provirus copies/diploid genome in presumed head-to-tail concatamers. In situ hybridization and immunohistochemical analysis revealed expression in approximately 5% of hepatocytes clustered in the pericentral region. These results support the use of AAV as a vector for diseases treatable by targeting of hepatocytes.
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Ahasan, Mohammad Shamim, Kuttichantran Subramaniam, Juan M. Campos Krauer, Katherine A. Sayler, Julia C. Loeb, Olivia F. Goodfriend, Hannah M. Barber, et al. "Three New Orbivirus Species Isolated from Farmed White-Tailed Deer (Odocoileus virginianus) in the United States." Viruses 12, no. 1 (December 20, 2019): 13. http://dx.doi.org/10.3390/v12010013.
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We report the detection and gene coding sequences of three novel Orbivirus species found in six dead farmed white-tailed deer in the United States. Phylogenetic analyses indicate that the new orbiviruses are genetically closely related to the Guangxi, Mobuck, Peruvian horse sickness, and Yunnan orbiviruses, which are thought to be solely borne by mosquitos. However, four of the six viruses analyzed in this work were found as co-infecting agents along with a known cervid pathogen, epizootic hemorrhagic disease virus-2 (EHDV-2), raising questions as to whether the new viruses are primary pathogens or secondary pathogens that exacerbate EHDV-2 infections. Moreover, EHDV-2 is known to be a Culicoides-borne virus, raising additional questions as to whether Culicoides species can also serve as vectors for the novel orbiviruses, if mosquitoes can vector EHDV-2, or whether the deer were infected through separate bites by the insects. Our findings expand knowledge of the possible viral pathogens of deer in the United States. Moreover, due to the close genetic relatedness of the three new orbiviruses to viruses that are primary pathogens of cattle and horses, our findings also underscore a crucial need for additional research on the potential role of the three new orbiviruses as pathogens of other animals.
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Romi, Hila, Oded Singer, Debora Rapaport, and Niza Frenkel. "Tamplicon-7, a Novel T-Lymphotropic Vector Derived from Human Herpesvirus 7." Journal of Virology 73, no. 8 (August 1, 1999): 7001–7. http://dx.doi.org/10.1128/jvi.73.8.7001-7007.1999.
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ABSTRACT We describe the derivation of a novel T-cell-defective virus vector employing the human herpesvirus 7 (HHV-7). The new vector, designated Tamplicon-7, replicates in CD4+ T cells. The system is composed of a helper virus and defective virus genomes derived by the replication of the input Tamplicon vector. There are twocis-acting functions required for the replication and packaging of the defective virus genomes in the presence of the helper virus: the viral DNA replication origin and the composite cleavage and packaging signal, which directs the cleavage and packaging of defective virus genomes. Viral DNA replication is compatible with the rolling circle mechanism, producing large head-to-tail concatemers of the Tamplicon vector. Thus, in the presence of the helper virus, the replicated vectors are packaged and secreted into the medium. Furthermore, we have shown that the vector can be employed to express a foreign gene, encoding the green fluorescent protein, in the T cells infected with the HHV-7 helper virus. We predict that the Tamplicon-7 vector might be potentially useful for gene therapy of diseases affecting the human CD4+ T cells, including autoimmune diseases, T-cell lymphomas, and AIDS.
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Gomme, Emily A., Elizabeth J. Faul, Phyllis Flomenberg, James P. McGettigan, and Matthias J. Schnell. "Characterization of a Single-Cycle Rabies Virus-Based Vaccine Vector." Journal of Virology 84, no. 6 (January 6, 2010): 2820–31. http://dx.doi.org/10.1128/jvi.01870-09.
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ABSTRACT Recombinant rabies virus (RV)-based vectors have demonstrated their efficacy in generating long-term, antigen-specific immune responses in murine and monkey models. However, replication-competent viral vectors pose significant safety concerns due to vector pathogenicity. RV pathogenicity is largely attributed to its glycoprotein (RV-G), which facilitates the attachment and entry of RV into host cells. We have developed a live, single-cycle RV by deletion of the G gene from an RV vaccine vector expressing HIV-1 Gag (SPBN-ΔG-Gag). Passage of SPBN-ΔG-Gag on cells stably expressing RV-G allowed efficient propagation of the G-deleted RV. The in vivo immunogenicity data comparing single-cycle RV to a replication-competent control (BNSP-Gag) showed lower RV-specific antibodies; however, the overall isotype profiles (IgG2a/IgG1) were similar for the two vaccine vectors. Despite this difference, mice immunized with SPBN-ΔG-Gag and BNSP-Gag mounted similar levels of Gag-specific CD8+ T-cell responses as measured by major histocompatibility complex class I Gag-tetramer staining, gamma interferon-enzyme-linked immunospot assay, and cytotoxic T-cell assay. Moreover, these cellular responses were maintained equally at immunization titers as low as 103 focus-forming units for both RV vaccine vectors. CD8+ T-cell responses were significantly enhanced by a boost with a single-cycle RV complemented with a heterologous vesicular stomatitis virus glycoprotein. These findings demonstrate that single-cycle RV is an effective alternative to replication-competent RV vectors for future development of vaccines for HIV-1 and other infectious diseases.
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Zouache, Karima, Albin Fontaine, Anubis Vega-Rua, Laurence Mousson, Jean-Michel Thiberge, Ricardo Lourenco-De-Oliveira, Valérie Caro, Louis Lambrechts, and Anna-Bella Failloux. "Three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential." Proceedings of the Royal Society B: Biological Sciences 281, no. 1792 (October 7, 2014): 20141078. http://dx.doi.org/10.1098/rspb.2014.1078.
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Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal of genetic and environmental contributions to vector competence. In particular, sources of variation do not necessarily act independently and may combine in the form of interactions. Here, we measured CHIKV transmission potential by the mosquito Aedes albopictus in all combinations of six worldwide vector populations, two virus strains and two ambient temperatures (20°C and 28°C). Overall, CHIKV transmission potential by Ae. albopictus strongly depended on the three-way combination of mosquito population, virus strain and temperature. Such genotype-by-genotype-by-environment (G × G × E) interactions question the relevance of vector competence studies conducted with a simpler set of conditions. Our results highlight the need to account for the complex interplay between vectors, pathogens and environmental factors to accurately assess the potential of vector-borne diseases to emerge.
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Rojas, Maria R., Monica A. Macedo, Minor R. Maliano, Maria Soto-Aguilar, Juliana O. Souza, Rob W. Briddon, Lawrence Kenyon, et al. "World Management of Geminiviruses." Annual Review of Phytopathology 56, no. 1 (August 25, 2018): 637–77. http://dx.doi.org/10.1146/annurev-phyto-080615-100327.
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Management of geminiviruses is a worldwide challenge because of the widespread distribution of economically important diseases caused by these viruses. Regardless of the type of agriculture, management is most effective with an integrated pest management (IPM) approach that involves measures before, during, and after the growing season. This includes starting with resistant cultivars and virus- and vector-free transplants and propagative plants. For high value vegetables, protected culture (e.g., greenhouses and screenhouses) allows for effective management but is limited owing to high cost. Protection of young plants in open fields is provided by row covers, but other measures are typically required. Measures that are used for crops in open fields include roguing infected plants and insect vector management. Application of insecticide to manage vectors (whiteflies and leafhoppers) is the most widely used measure but can cause undesirable environmental and human health issues. For annual crops, these measures can be more effective when combined with host-free periods of two to three months. Finally, given the great diversity of the viruses, their insect vectors, and the crops affected, IPM approaches need to be based on the biology and ecology of the virus and vector and the crop production system. Here, we present the general measures that can be used in an IPM program for geminivirus diseases, specific case studies, and future challenges.
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Mastakov, Mihail Y., Kristin Baer, C. Wymond Symes, Claudia B. Leichtlein, Robert M. Kotin, and Matthew J. During. "Immunological Aspects of Recombinant Adeno-Associated Virus Delivery to the Mammalian Brain." Journal of Virology 76, no. 16 (August 15, 2002): 8446–54. http://dx.doi.org/10.1128/jvi.76.16.8446-8454.2002.
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ABSTRACT Recombinant adeno-associated viruses (rAAV) are highly efficient vectors for gene delivery into the central nervous system (CNS). However, host inflammatory and immune responses may play a critical role in limiting the use of rAAV vectors for gene therapy and functional genomic studies in vivo. Here, we evaluated the effect of repeated injections of five rAAV vectors expressing different genetic sequences (coding or noncoding) in a range of combinations into the rat brain. Specifically, we wished to determine whether a specific immune or inflammatory response appeared in response to the vector and/or the transgene protein after repeated injections under conditions of mannitol coinjection. We show that readministration of the same rAAV to the CNS is possible if the interval between the first and second injection is more than 4 weeks. Furthermore, our data demonstrate that rAAV vectors carrying different genetic sequences can be administered at intervals of 2 weeks. Our data therefore suggest that the AAV capsid structure is altered by the vector genetic sequence, such that secondary structures of the single-stranded genome have an impact on the antigenicity of the virus. This study provides guidelines for more rational design of gene transfer studies in the rodent brain and, in addition, suggests the use of repeated administration of rAAV as a viable form of therapy for the treatment of chronic diseases.