Academic literature on the topic 'Insertion elements, DNA'

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Journal articles on the topic "Insertion elements, DNA"

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Hoogland, Christine, and Christian Biémont. "Chromosomal Distribution of Transposable Elements in Drosophila melanogaster Test of the Ectopic Recombination Model for Maintenance of Insertion Site Number." Genetics 144, no. 1 (September 1, 1996): 197–204. http://dx.doi.org/10.1093/genetics/144.1.197.

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Abstract Data of insertion site localization and site occupancy frequency of P, hobo, I, copia, mdg1, mdg3, 412, 297, and roo transposable elements (TEs) on the polytene chromosomes of Drosophila melanogaster were extracted from the literature. We show that TE insertion site number per chromosomal division was significantly correlated with the amount of DNA. The insertion site number weighted by DNA content was not correlated with recombination rate for all TEs except hobo, for which a positive correlation was detected. No global tendency emerged in the relationship between TE site occupancy f
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Wuitschick, Jeffrey D., Paul R. Lindstrom, Alison E. Meyer, and Kathleen M. Karrer. "Homing Endonucleases Encoded by Germ Line-Limited Genes in Tetrahymena thermophila Have APETELA2 DNA Binding Domains." Eukaryotic Cell 3, no. 3 (June 2004): 685–94. http://dx.doi.org/10.1128/ec.3.3.685-694.2004.

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ABSTRACT Three insertion elements were previously found in a family of germ line-limited mobile elements, the Tlr elements, in the ciliate Tetrahymena. Each of the insertions contains an open reading frame (ORF). Sequence analysis of the deduced proteins encoded by the elements suggests that they are homing endonucleases. The genes are designated TIE1-1, TIE2-1, and TIE3-1 for Tetrahymena insertion-homing endonuclease. The endonuclease motif occupies the amino terminal half of each TIE protein. The C-terminal regions of the proteins are similar to the APETELA2 DNA binding domain of plant trans
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Ryan, Margret, Jerry D. Johnson, and Lee A. Bulla Jr. "Insertion sequence elements in Bacillus thuringiensis subsp. darmstadiensis." Canadian Journal of Microbiology 39, no. 7 (July 1, 1993): 649–58. http://dx.doi.org/10.1139/m93-094.

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Two variants of insertion sequence IS231, named IS231G and H, were isolated from Bacillus thuringiensis subsp. darmstadiensis 73-E-10-2 (BTD2), an isolate toxic to dipteran insects, and characterized by DNA sequence analysis. They are encoded consecutively as direct repeats on an EcoRI fragment of 5.6 kilo base pairs. Direct tandem repeats of IS231 elements have not been previously reported. Both elements are closely related to other members of the IS231 family that have been isolated from B. thuringiensis strains toxic to lepidopteran as well as to dipteran insects. A close correlation exists
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Chalker, D. L., and S. B. Sandmeyer. "Transfer RNA genes are genomic targets for de Novo transposition of the yeast retrotransposon Ty3." Genetics 126, no. 4 (December 1, 1990): 837–50. http://dx.doi.org/10.1093/genetics/126.4.837.

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Abstract Insertions of the yeast element Ty3 resulting from induced retrotransposition were characterized in order to identify the genomic targets of transposition. The DNA sequences of the junctions between Ty3 and flanking DNA were determined for two insertions of an unmarked element. Each insertion was at position -17 from the 5' end of a tRNA-coding sequence. Ninety-one independent insertions of a marked Ty3 element were studied by Southern blot analysis. Pairs of independent insertions into seven genomic loci accounted for 14 of these insertions. The DNA sequence flanking the insertion si
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Yin, Bin, and David A. Largaespada. "PCR-based procedures to isolate insertion sites of DNA elements." BioTechniques 43, no. 1 (July 2007): 79–84. http://dx.doi.org/10.2144/000112474.

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Paskewitz, Susan M., and Frank H. Collins. "Site-specific ribosomal DNA insertion elements inAnopheles gambiaeandA.arbiensis: nucleotide sequence of gene-element boundaries." Nucleic Acids Research 17, no. 20 (1989): 8125–33. http://dx.doi.org/10.1093/nar/17.20.8125.

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Voelker, R. A., J. Graves, W. Gibson, and M. Eisenberg. "Mobile element insertions causing mutations in the Drosophila suppressor of sable locus occur in DNase I hypersensitive subregions of 5'-transcribed nontranslated sequences." Genetics 126, no. 4 (December 1, 1990): 1071–82. http://dx.doi.org/10.1093/genetics/126.4.1071.

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Abstract The locations of 16 mobile element insertions causing mutations at the Drosophila suppressor of sable [su(s)] locus were determined by restriction mapping and DNA sequencing of the junction sites. The transposons causing the mutations are: P element (5 alleles), gypsy (3 alleles), 17.6, HMS Beagle, springer, Delta 88, prygun, Stalker, and a new mobile element which was named roamer (2 alleles). Four P element insertions occur in 5' nontranslated leader sequences, while the fifth P element and all 11 non-P elements inserted into the 2053 nucleotide, 5'-most intron that is spliced from
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8

Gosselin, Sophia P., Danielle R. Arsenault, Catherine A. Jennings, and Johann Peter Gogarten. "The Evolutionary History of a DNA Methylase Reveals Frequent Horizontal Transfer and Within-Gene Recombination." Genes 14, no. 2 (January 21, 2023): 288. http://dx.doi.org/10.3390/genes14020288.

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Inteins, often referred to as protein introns, are highly mobile genetic elements that invade conserved genes throughout the tree of life. Inteins have been found to invade a wide variety of key genes within actinophages. While in the process of conducting a survey of these inteins in actinophages, we discovered that one protein family of methylases contained a putative intein, and two other unique insertion elements. These methylases are known to occur commonly in phages as orphan methylases (possibly as a form of resistance to restriction–modification systems). We found that the methylase fa
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Woods, Wayne G., Katrina Ngui, and Michael L. Dyall-Smith. "An Improved Transposon for the Halophilic ArchaeonHaloarcula hispanica." Journal of Bacteriology 181, no. 22 (November 15, 1999): 7140–42. http://dx.doi.org/10.1128/jb.181.22.7140-7142.1999.

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ABSTRACT An improved transposon (ThD73) for Haloarcula hispanica is described. Based on the halobacterial insertion sequence ISH28, it showed little target sequence specificity but was biased toward a lower G+C content. Twenty randomly selected ThD73 mutants were analyzed, and the DNA flanking their insertions revealed several recognizable sequences, including two (unrelated) ISH elements.
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Hargrove, Phillip W., Steven Kepes, Hideki Hanawa, Cheng Cheng, Geoff Neale, Arthur W. Nienhuis, and Derek A. Persons. "Assessment of Changes in Gene Expression Caused by Insertions of a Globin Lentiviral Vector Containing Globin Regulatory Elements or a Lentiviral Vector Containing Retroviral LTR Elements." Blood 104, no. 11 (November 16, 2004): 497. http://dx.doi.org/10.1182/blood.v104.11.497.497.

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Abstract The development of lymphoid leukemia in two children with X-SCID who underwent gene therapy was partially due to activation of the LMO-2 proto-oncogene by the retroviral LTR of the vector which inserted nearby (Hacein-Bey-Abina et al., Science 2003), highlighting the importance of vector design on the potential to activate genes near vector integration sites. As gene therapy vectors for other blood disorders are evaluated, it seems prudent to assess the safety issues regarding insertion for each particular vector in appropriate pre-clinical models. We have focused on developing γ-glob
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Dissertations / Theses on the topic "Insertion elements, DNA"

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Harris, Linda Janice. "Characterization of the Caenorhabditis elegans var. Bristol (strain N2) Tc1 elements and related transposable elements in Caenorhabditis briggsae." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/28838.

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The regulation and evolution of the inverted repeat transposable element Tel, found in the nematode Caenorhabditis elegans, was studied. The stability of Tel elements in the N2 strain genome was investigated by cloning seventeen N2 Tel elements. To examine their structural integrity, sixteen cloned N2 Tel elements were restriction mapped and, in the case of some variants, their DNA was partially sequenced. Two restriction site variants, Tcl(Eco).12 and Tcl(Hpa-).9, were found. Tel(1.5).10b had lost 89 bp from one end, while Tcl(1.7).28 contained a 55 bp insertion. Two additional elements, Tcl(
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Raghavan, Rahul. "Mobile genetic elements in coxiella burnetii friends, foes or just indifferent? /." [Missoula, Mont.] : The University of Montana, 2008. http://etd.lib.umt.edu/theses/available/etd-12092008-141715/unrestricted/umi-umt-1105.pdf.

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Fobert, Pierre R. (Pierre Rheal) Carleton University Dissertation Biology. "Characterization of chromosomal sites of T-DNA integration by activation of a promoterless B-glucuronidase (GUS) gene linked to the T-DNA right border repeat." Ottawa, 1992.

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Bhangale, Tushar. "Small insertion-deletion polymorphisms in the human genome : characterization and automation of detection by resequencing /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/8044.

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Pillai, Suresh Divakaran. "Ecology and genetic stability of Tn5 mutants of bean rhizobia in Sonoran desert soils." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184823.

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Five transposon Tn5 mutants of bean rhizobia (Rhizobium leguminosarum b.v. phaseoli) and the wild type strain were used in ecological studies to evaluate the efficacy of transposon Tn5 as a phenotypic marker in rhizobia for ecological studies in two Sonoran desert soils. All mutants possessed chromosomal insertions of the transposable element. Survival of each mutant strain was compared to that of the wild type strain under non stress, moisture stress and temperature stress conditions in Pima silty clay loam and Brazil to sandy loam. The genetic stability of Tn5 in terms of transposition of th
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Liu, Xiulan. "Characterisation of antibiotic resistance gene clusters and their mobility within a collection of multi-drug resistant Salmonella spp." School of Biological Sciences - Faculty of Science, 2009. http://ro.uow.edu.au/theses/3043.

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One hundred and thirty-six Salmonella enterica strains, isolated from humans, animals, environmental and plant sources in Australia from 23 serovars, were examined for the streptomycin resistance gene strA and strB, the sulfonamide resistance gene sul2, and the tetracycline resistance gene tetA(A) and tetA(B). Thirteen strains were identified as containing the strA-strB genes located on the transposon Tn5393. S. enterica serovar Hadar accounted for 11 of these strains, 6 of which were isolated from humans and 5 were from ducks. This investigation is therefore the first report of the Tn5393 tra
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Signorelli, Katherine Louise. "Characterization of an insertional mutation in a line of transgenic mice /." Access full-text from WCMC, 1989. http://proquest.umi.com/pqdweb?did=744576231&sid=1&Fmt=2&clientId=8424&RQT=309&VName=PQD.

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Richter, Grace Yukiko. "Molecular characterization of specificity and activity of the transposable element IS801." Thesis, 1995. http://hdl.handle.net/1957/34682.

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1S801 is a transposable element isolated from Pseudomonas syringae pathovar (pv.) phaseolicola, the causal agent of halo blight of bean. Fragments of the element are present in multiple copies on an indigenous plasmid, pMMC7105, of strain LR781, and have been implicated as sites of homologous recombination leading to imprecise excision of the chromosomally integrated form of the plasmid. The element, which has been completely sequenced, is 1512 base pairs in length and is unusual among transposable elements in that it does not have direct or inverted repeats in its termini. The terminal region
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Brezinsky, Laura. "Molecular and evolutionary characterization of the transposable element Uhu from Hawaiian Drosophila." Thesis, 1990. http://hdl.handle.net/10125/9397.

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Shim, Je-Seop. "Studies on the agrocin 84 plasmid of `Agrobacterium radiobacter`." 1987. http://web4.library.adelaide.edu.au/theses/09PH/09phs5565.pdf.

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Books on the topic "Insertion elements, DNA"

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Barbara, McClintock. The discovery and characterization of transposable elements: The collected papers of Barbara McClintock. New York: Garland Pub., 1987.

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Wisconsin-Madison), International Symposium on Plant Transposable Elements (1987 University of. Plant transposable elements. New York: Plenum Press, 1988.

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Mobile DNA: Finding treasure in junk. Upper Saddle River, New Jersey: FT Press, 2011.

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Richter, Grace Yukiko. Molecular characterization of specificity and activity of the transposable element IS801. 1995.

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H, Saedler, and Gierl A, eds. Transposable elements. Berlin: Springer-Verlag, 1996.

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E, Lambert Michael, McDonald John F. 1947-, Weinstein I. Bernard, Cold Spring Harbor Laboratory, and Abbott Laboratories, eds. Eukaryotic transposable elements as mutagenic agents. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory, 1988.

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Sniegowski, Paul D. Transposable elements and polymorphic inversions in Drosophila melanogaster. 1993.

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Lambert, Michael E., and John F. McDonald. Eukaryotic Transposable Elements As Mutagenic Agents (Banbury Report) (Banbury Report). Cold Spring Harbor Laboratory Pr, 1988.

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Plant DNA infectious agents. Wien: Springer-Verlag, 1987.

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Plant Transposable Elements Topics in Current Genetics. Springer, 2012.

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Book chapters on the topic "Insertion elements, DNA"

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Wessler, Susan R., George Baran, and Marguerite Varagona. "Alterations in Gene Expression Mediated by DNA Insertions in the waxy Gene of Maize." In Plant Transposable Elements, 293–303. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5550-2_22.

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Zhou, Bo, Michael S. Haney, Xiaowei Zhu, Reenal Pattni, Alexej Abyzov, and Alexander E. Urban. "Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues Using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants." In Methods in Molecular Biology, 173–90. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_11.

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Cassidy, Annelise, and Stephane Pelletier. "Emerging CRISPR Technologies." In CRISPR Technology [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.106652.

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The discovery and implementation of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas) systems for genome editing has revolutionized biomedical research and holds great promise for the treatment of human genetic disorders. In addition to the popular CRISPR-Cas9 and CRISPR-Cpf1 systems for genome editing, several additional Class I and Class 2 CRISPR-Cas effectors have been identified and adapted for genome editing and transcriptome modulation. Here we discuss current and emerging CRISPR-based technologies such as Cascade-Cas3, CRISPR-associated transposases (CAST), CRISPR-Cas7–11, and CRISPR-Cas13 for genome and transcriptome modification. These technologies allow for the removal or insertion of large DNA elements, the modulation of gene expression at the transcriptional level, and the editing of RNA transcripts, expanding the capabilities of current technologies.
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da Silva, Maelin, Daniele Aparecida Matoso, Vladimir Pavan Margarido, Eliana Feldberg, and Roberto Ferreira Artoni. "Composition and Nature of Heterochromatin in the Electrical Fish (Knifefishes) Gymnotus (Gymnotiformes: Gymnotidae)." In Cytogenetics - Classical and Molecular Strategies for Analysing Heredity Material. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.97673.

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Fishes of the genus Gymnotus have been suggested as a good model for biogeographic studies in the South American continent. In relation to heterochromatin, species of this genus have blocks preferably distributed in the centromeric region. The content of these regions has been shown to be variable, with description of transposable elements, pseudogenes of 5S rDNA and satellite sequences. In G. carapo Clade, although geographically separated, species with 2n = 54 chromosomes share the distribution of many 5S rDNA sites, a unique case within the genus. Here, repetitive DNA sequences from G. sylvius (2n = 40) and G. paraguensis (2n = 54) were isolated and mapped to understand their constitution. The chromosome mapping by FISH showed an exclusive association in the centromeres of all chromosomes. However, the cross-FISH did not show positive signs of interspecific hybridization, indicating high levels of heterochromatic sequence specificity. In addition, COI-1 sequences were analyzed in some species of Gymnotus, which revealed a close relationship between species of clade 2n = 54, which have multiple 5S rDNA sites. Possibly, the insertion of retroelements or pseudogenization and dispersion of this sequence occurred before the geographic dispersion of the ancestor of this clade from the Amazon region to the hydrographic systems of Paraná-Paraguay, a synapomorphy for the group.
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Conference papers on the topic "Insertion elements, DNA"

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Deviprasad, T., and T. Kesavadas. "VPAVE: An Interactive Tool for Validating Assembly Components in Virtual Environment Using Finite Element Simulation." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0165.

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Abstract Assembly is a geometric problem and its success depends on the quality of the mating parts. Design for Assembly (DFA) takes care of the issues such as part handling, insertion and mating, and other features that make assembly an easier and cost effective task. Practical considerations like part deformation during manufacturing, wear and tear of machines and jigs, and other constraints like cost and technical limitations contribute significantly to dimensional and form errors. These factors are usually not accounted for during the DFA, as the data is not as yet available. This results
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Yoon, Kyungmin, Chansu Jang, and Jooil Yoon. "A Study on Doppler Weighting Factor for Control Element Assembly Ejection Accident by Using Newly Developed Nuclear Design Code and Non-LOCA Methodology." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65996.

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Among Reactivity Initiated Accidents (RIAs) for Pressurized Water Reactor (PWR), Control Element Assembly Ejection (CEAE) accident causes the rapid positive reactivity insertion to the core. It causes an asymmetric power distortion which results in the rising of local fuel temperature, fuel pellet thermal expansion and cladding ballooning or rupture. In the CEAE accident, Doppler feedback has a profound effect because the negative reactivity insertion due to the rise of fuel temperature reduces the core power after rapid power excursion. But the Doppler reactivity can’t be calculated properly
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Reports on the topic "Insertion elements, DNA"

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Epel, Bernard, and Roger Beachy. Mechanisms of intra- and intercellular targeting and movement of tobacco mosaic virus. United States Department of Agriculture, November 2005. http://dx.doi.org/10.32747/2005.7695874.bard.

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To cause disease, plant viruses must replicate and spread locally and systemically within the host. Cell-to-cell virus spread is mediated by virus-encoded movement proteins (MPs), which modify the structure and function of plasmodesmata (Pd), trans-wall co-axial membranous tunnels that interconnect the cytoplasm of neighboring cells. Tobacco mosaic virus (TMV) employ a single MP for cell- cell spread and for which CP is not required. The PIs, Beachy (USA) and Epel (Israel) and co-workers, developed new tools and approaches for study of the mechanism of spread of TMV that lead to a partial iden
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