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Journal articles on the topic 'Integrative conjugative element (ICE)'

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Published: 8 June 2024
Last updated: 31 July 2025

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1

Iannelli, Francesco, Francesco Santoro, Marco R. Oggioni, and Gianni Pozzi. "Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253of Streptococcus pneumoniae." Antimicrobial Agents and Chemotherapy 58, no. 2 (2013): 1235–39. http://dx.doi.org/10.1128/aac.01764-13.

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ABSTRACTConjugative transposon Tn5253, an integrative conjugative element (ICE) ofStreptococcus pneumoniaecarrying thecatandtet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251(18,033 bp), of the Tn916-Tn1545family of ICEs, and Ωcat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253was observed whenSt
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2

Libante, Virginie, Nazim Sarica, Abbas Mohamad Ali, et al. "Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins." Genes 11, no. 9 (2020): 1004. http://dx.doi.org/10.3390/genes11091004.

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Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are in
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3

Giovanetti, Eleonora, Andrea Brenciani, Erika Tiberi, Alessandro Bacciaglia, and Pietro Emanuele Varaldo. "ICESp2905, theerm(TR)-tet(O) Element of Streptococcus pyogenes, Is Formed by Two Independent Integrative and Conjugative Elements." Antimicrobial Agents and Chemotherapy 56, no. 1 (2011): 591–94. http://dx.doi.org/10.1128/aac.05352-11.

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ABSTRACTIn ICESp2905, a widespreaderm(TR)- andtet(O)-carrying genetic element ofStreptococcus pyogenes, the two resistance determinants are contained in separate fragments inserted into a scaffold of clostridial origin. ICESp2905(∼65.6 kb) was transferable not only in its regular form but also in a defective form lacking theerm(TR) fragment (ICESp2906, ∼53.0 kb). Theerm(TR) fragment was also an independent integrative and conjugative element (ICE) (ICESp2907, ∼12.6 kb). ICESp2905thus results from one ICE (ICESp2907) being integrated into another (ICESp2906).
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Del Grosso, Maria, Romina Camilli, Ermanno Rizzi, Alessandro Pietrelli, Gianluca De Bellis, and Annalisa Pantosti. "ICESpy009, a Conjugative Genetic Element Carryingmef(E) in Streptococcus pyogenes." Antimicrobial Agents and Chemotherapy 60, no. 7 (2016): 3906–12. http://dx.doi.org/10.1128/aac.03082-15.

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ABSTRACTEfflux-mediated macrolide resistance due tomef(E) andmel, carried by the mega element, is common inStreptococcus pneumoniae, for which it was originally characterized, but it is rare inStreptococcus pyogenes. InS. pyogenes, mega was previously found to be enclosed in Tn2009, a composite genetic element of the Tn916family containingtet(M) and conferring erythromycin and tetracycline resistance. In this study,S. pyogenesisolates containingmef(E), apparently not associated with other resistance determinants, were examined to characterize the genetic context of mega. By whole-genome sequen
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5

Michael, G. B., K. Kadlec, M. T. Sweeney, et al. "ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer." Journal of Antimicrobial Chemotherapy 67, no. 1 (2011): 91–100. http://dx.doi.org/10.1093/jac/dkr411.

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6

Auchtung, Jennifer M., Naira Aleksanyan, Artemisa Bulku, and Melanie B. Berkmen. "Biology of ICE Bs1 , an integrative and conjugative element in Bacillus subtilis." Plasmid 86 (July 2016): 14–25. http://dx.doi.org/10.1016/j.plasmid.2016.07.001.

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7

Calcutt, Michael J., Michelle S. Lewis, and Kim S. Wise. "Molecular Genetic Analysis of ICEF, an Integrative Conjugal Element That Is Present as a Repetitive Sequence in the Chromosome of Mycoplasma fermentans PG18." Journal of Bacteriology 184, no. 24 (2002): 6929–41. http://dx.doi.org/10.1128/jb.184.24.6929-6941.2002.

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ABSTRACT Mycoplasma genomes contain compact gene sets that approach the minimal complement necessary for life and reflect multiple evolutionary instances of genomic reduction. Lateral gene transfer may play a critical role in shaping the mobile gene pool in these organisms, yet complex mobile elements have not been reported within this genus. We describe here a large (∼23-kb) genetic element with unique features that is present in four copies in the Mycoplasma fermentans PG18 chromosome, accounting for approximately 8% of the genome. These novel elements, designated ICEF (integrative conjugal
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8

Selyanskaya, N. A., S. V. Titova, E. A. Menshikova, S. O. Vodopyanov, and V. D. Kruglikov. "Influence of Cultivation Conditions on the Transmission of Antibiotic Resistance Genes in Vibrio cholera." Antibiot Khimioter = Antibiotics and Chemotherapy 69, no. 9-10 (2025): 4–10. https://doi.org/10.37489/0235-2990-2024-69-9-10-4-10.

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The growth of antibiotic resistance necessitates studying the processes of acquisition and loss of genetic elements responsible for resistance. The aim of the study was to investigate the effect of temperature, biofilm formation, and antibiotics on the efficiency of integrative conjugative element (ICE) transfer in Vibrio cholerae O1 El Tor strains. Material and methods. Conjugative transfer of the ICE element from V. cholerae O1 El Tor strains to Escherichia сoli QD 5003 Rifr and V. cholera O1 El Tor 5879 Nalr cells was carried out in plankton and in biofilms on plastic and chitin at 25–37°C.
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9

Levicán, Gloria J., Assaf Katz, Jorge H. Valdés, Raquel Quatrini, David S. Holmes, and O. Orellana. "A 300 kpb Genome Segment, Including a Complete Set of tRNA Genes, is Dispensable for Acidithiobacillus Ferrooxidans." Advanced Materials Research 71-73 (May 2009): 187–90. http://dx.doi.org/10.4028/www.scientific.net/amr.71-73.187.

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The genome sequences from two strains of the acidophilic, autotrophic, chemolithotrophic proteobacterium A. ferrooxidans are available from genome databases. Bioinformatic sequence comparison revealed the existence in one strain of a putative integrative conjugative element (ICE), containing an entire set of clustered tRNA genes. ICE is missing in the other strain, suggesting that this element as well as the tRNA genes cluster is dispensable for the bacterium. Bioinformatic predictions suggest that the tRNA genes cluster might mainly contribute to the translation of ICE encoded genes.
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10

McKeithen-Mead, Saria A., and Alan D. Grossman. "Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host." PLOS Genetics 19, no. 2 (2023): e1010524. http://dx.doi.org/10.1371/journal.pgen.1010524.

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Integrative and conjugative elements (ICEs) are major contributors to genome plasticity in bacteria. ICEs reside integrated in the chromosome of a host bacterium and are passively propagated during chromosome replication and cell division. When activated, ICEs excise from the chromosome and may be transferred through the ICE-encoded conjugation machinery into a recipient cell. Integration into the chromosome of the new host generates a stable transconjugant. Although integration into the chromosome of a new host is critical for the stable acquisition of ICEs, few studies have directly investig
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11

Aberkane, Salim, Fabrice Compain, Dominique Decré, et al. "High Prevalence of SXT/R391-Related Integrative and Conjugative Elements CarryingblaCMY-2in Proteus mirabilis Isolates from Gulls in the South of France." Antimicrobial Agents and Chemotherapy 60, no. 2 (2015): 1148–52. http://dx.doi.org/10.1128/aac.01654-15.

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ABSTRACTThe genetic structures involved in the dissemination ofblaCMY-2carried byProteus mirabilisisolates recovered from different gull species in the South of France were characterized and compared to clinical isolates.blaCMY-2was identified inP. mirabilisisolates from 27/93 yellow-legged gulls and from 37/65 slender-billed gulls. It was carried by a conjugative SXT/R391-like integrative and conjugative element (ICE) in all avian strains and in 3/7 human strains. Two clinical isolates had the same genetic background as six avian isolates.
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12

Sulser, Sandra, Andrea Vucicevic, Veronica Bellini, et al. "A bistable prokaryotic differentiation system underlying development of conjugative transfer competence." PLOS Genetics 18, no. 6 (2022): e1010286. http://dx.doi.org/10.1371/journal.pgen.1010286.

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The mechanisms and impact of horizontal gene transfer processes to distribute gene functions with potential adaptive benefit among prokaryotes have been well documented. In contrast, little is known about the life-style of mobile elements mediating horizontal gene transfer, whereas this is the ultimate determinant for their transfer fitness. Here, we investigate the life-style of an integrative and conjugative element (ICE) within the genus Pseudomonas that is a model for a widespread family transmitting genes for xenobiotic compound metabolism and antibiotic resistances. Previous work showed
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13

Bean, Emily L., Calvin Herman, Mary E. Anderson, and Alan D. Grossman. "Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies." PLOS Genetics 18, no. 5 (2022): e1009998. http://dx.doi.org/10.1371/journal.pgen.1009998.

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Integrative and conjugative elements (ICEs) are mobile genetic elements that reside in a bacterial host chromosome and are prominent drivers of bacterial evolution. They are also powerful tools for genetic analyses and engineering. Transfer of an ICE to a new host involves many steps, including excision from the chromosome, DNA processing and replication, transfer across the envelope of the donor and recipient, processing of the DNA, and eventual integration into the chromosome of the new host (now a stable transconjugant). Interactions between an ICE and its host throughout the life cycle lik
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14

Bidet, Philippe, Romain Basmaci, Julien Guglielmini та ін. "Genome Analysis of Kingella kingae Strain KWG1 Reveals How a β-Lactamase Gene Inserted in the Chromosome of This Species". Antimicrobial Agents and Chemotherapy 60, № 1 (2015): 703–8. http://dx.doi.org/10.1128/aac.02192-15.

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ABSTRACTWe describe the genome of a penicillinase-producingKingella kingaestrain (KWG1), the first to be isolated in continental Europe, whoseblaTEM-1gene was, for the first time in this species, found to be chromosomally inserted. TheblaTEMgene is located in an integrative and conjugative element (ICE) inserted in Met-tRNA and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. This ICE is homologous to resistance-conferring plasmids ofK. kingaeand other Gram-negative bacteria.
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15

Tardy, Florence, Virginie Mick, Emilie Dordet-Frisoni, et al. "Integrative Conjugative Elements Are Widespread in Field Isolates of Mycoplasma Species Pathogenic for Ruminants." Applied and Environmental Microbiology 81, no. 5 (2014): 1634–43. http://dx.doi.org/10.1128/aem.03723-14.

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ABSTRACTComparative genomics have revealed massive horizontal gene transfer (HGT) betweenMycoplasmaspecies sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogenMycoplasma agalactiae. To estimate the prevalence of ICEs in ruminant mycoplasmas, we surveyed their occurrence in a collection of 166 field strains representing 4 (sub)species that are recognized as major pathogens. Based on available sequenced genomes, we first defined the conserved, minimal ICE backbone as composed of 4
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16

Shang, Yanhong, Dexi Li, Wenbo Hao, et al. "A prophage and two ICESa2603-family integrative and conjugative elements (ICEs) carrying optrA in Streptococcus suis." Journal of Antimicrobial Chemotherapy 74, no. 10 (2019): 2876–79. http://dx.doi.org/10.1093/jac/dkz309.

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Abstract Objectives To investigate the presence and transfer of the oxazolidinone/phenicol resistance gene optrA and identify the genetic elements involved in the horizontal transfer of the optrA gene in Streptococcus suis. Methods A total of 237 S. suis isolates were screened for the presence of the optrA gene by PCR. Whole-genome DNA of three optrA-positive strains was completely sequenced using the Illumina MiSeq and Pacbio RSII platforms. MICs were determined by broth microdilution. Transferability of the optrA gene in S. suis was investigated by conjugation. The presence of circular inter
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17

Roche, David, Maud Fléchard, Nathalie Lallier, et al. "ICEEc2, a New Integrative and Conjugative Element Belonging to the pKLC102/PAGI-2 Family, Identified in Escherichia coli Strain BEN374." Journal of Bacteriology 192, no. 19 (2010): 5026–36. http://dx.doi.org/10.1128/jb.00609-10.

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ABSTRACT The diversity of the Escherichia coli species is in part due to the large number of mobile genetic elements that are exchanged between strains. We report here the identification of a new integrative and conjugative element (ICE) of the pKLC102/PAGI-2 family located downstream of the tRNA gene pheU in the E. coli strain BEN374. Indeed, this new region, which we called ICEEc2, can be transferred by conjugation from strain BEN374 to the E. coli strain C600. We were also able to transfer this region into a Salmonella enterica serovar Typhimurium strain and into a Yersinia pseudotuberculos
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18

Han, Xiaoyan, Xiang-Dang Du, Luke Southey, et al. "Functional Analysis of a Bacitracin Resistance Determinant Located on ICECp1, a Novel Tn916-Like Element from a Conjugative Plasmid in Clostridium perfringens." Antimicrobial Agents and Chemotherapy 59, no. 11 (2015): 6855–65. http://dx.doi.org/10.1128/aac.01643-15.

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ABSTRACTBacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. They act by interfering with the biosynthesis of the bacterial cell wall. In this study, we analyzed an avian necrotic enteritis strain ofClostridium perfringensthat was resistant to bacitracin and produced NetB toxin. We identified a bacitracin resistance locus that resembled a bacitracin resistance determinant fromEnterococcus faecalis. It contained the structural genesbcrABDand a putative regulatory gene,bcrR. Mutagenesis studies provided evidence that bothbcrAandbcrBare essential for ba
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19

Kong, Ling-Han, Rong Xiang, Yu-Long Wang, et al. "Integration of the blaNDM-1 carbapenemase gene into a novel SXT/R391 integrative and conjugative element in Proteus vulgaris." Journal of Antimicrobial Chemotherapy 75, no. 6 (2020): 1439–42. http://dx.doi.org/10.1093/jac/dkaa068.

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Abstract Objectives To characterize the genetic environment of the carbapenem resistance determinant in Proteus vulgaris of swine origin. Methods The carbapenem-resistant P. vulgaris strain BC22 was isolated from a faecal swab from a diseased pig with diarrhoea in Sichuan Province of China in 2018. The presence of carbapenemase genes was screened by PCR. WGS and bioinformatics analysis were performed to analyse the genetic environment of the carbapenem resistance determinant. Results P. vulgaris strain BC22 was found to harbour the carbapenemase gene blaNDM-1. WGS data revealed that blaNDM-1 w
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20

Lei, Chang-Wei, An-Yun Zhang, Hong-Ning Wang, Bi-Hui Liu, Li-Qin Yang, and Yong-Qiang Yang. "Characterization of SXT/R391 Integrative and Conjugative Elements in Proteus mirabilis Isolates from Food-Producing Animals in China." Antimicrobial Agents and Chemotherapy 60, no. 3 (2016): 1935–38. http://dx.doi.org/10.1128/aac.02852-15.

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SXT/R391 integrative and conjugative elements (ICEs) were detected in 8 out of 125Proteus mirabilisisolates from food-producing animals in China. Whole-genome sequencing revealed that seven ICEs were identical to ICEPmiJpn1, carrying the cephalosporinase geneblaCMY-2. Another one, designated ICEPmiChn1, carried five resistance genes. All eight ICEs could be transferred toEscherichia colivia conjugation. The results highlight the idea that animal farms are important reservoir of the SXT/R391 ICE-containingP. mirabilis.
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Sheahan, Madeline L., Katia Flores, Michael J. Coyne, et al. "A ubiquitous mobile genetic element changes the antagonistic weaponry of a human gut symbiont." Science 386, no. 6720 (2024): 414–20. http://dx.doi.org/10.1126/science.adj9504.

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DNA transfer is ubiquitous in the human gut microbiota, especially among species of the order Bacteroidales. In silico analyses have revealed hundreds of mobile genetic elements shared between these species, yet little is known about the phenotypes they encode, their effects on fitness, or pleiotropic consequences for the recipient’s genome. In this work, we show that acquisition of a ubiquitous integrative conjugative element (ICE) encoding a type VI secretion system (T6SS) shuts down the native T6SS of Bacteroides fragilis . Despite inactivating this T6SS, ICE acquisition increases the fitne
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22

Delavat, François, Sara Mitri, Serge Pelet, and Jan Roelof van der Meer. "Highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element." Proceedings of the National Academy of Sciences 113, no. 24 (2016): E3375—E3383. http://dx.doi.org/10.1073/pnas.1604479113.

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Horizontal gene transfer is an important evolutionary mechanism for bacterial adaptation. However, given the typical low transfer frequencies in a bacterial population, little is known about the fate and interplay of donor cells and the mobilized DNA during transfer. Here we study transfer of an integrative and conjugative element (ICE) among individual live bacterial cells. ICEs are widely distributed mobile DNA elements that are different than plasmids because they reside silent in the host chromosome and are maintained through vertical descent. Occasionally, ICEs become active, excise, and
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Davies, Mark R., Josephine Shera, Gary H. Van Domselaar, Kadaba S. Sriprakash та David J. McMillan. "A Novel Integrative Conjugative Element Mediates Genetic Transfer from Group G Streptococcus to Other β-Hemolytic Streptococci". Journal of Bacteriology 191, № 7 (2009): 2257–65. http://dx.doi.org/10.1128/jb.01624-08.

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ABSTRACT Lateral gene transfer is a significant contributor to the ongoing evolution of many bacterial pathogens, including β-hemolytic streptococci. Here we provide the first characterization of a novel integrative conjugative element (ICE), ICESde3396, from Streptococcus dysgalactiae subsp. equisimilis (group G streptococcus [GGS]), a bacterium commonly found in the throat and skin of humans. ICESde3396 is 64 kb in size and encodes 66 putative open reading frames. ICESde3396 shares 38 open reading frames with a putative ICE from Streptococcus agalactiae (group B streptococcus [GBS]), ICESa26
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Burrus, Vincent, and Matthew K. Waldor. "Formation of SXT Tandem Arrays and SXT-R391 Hybrids." Journal of Bacteriology 186, no. 9 (2004): 2636–45. http://dx.doi.org/10.1128/jb.186.9.2636-2645.2004.

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ABSTRACT SXT is an integrative and conjugative element (ICE) isolated from Vibrio cholerae. This ∼100-kb ICE encodes resistance to multiple antibiotics and integrates site specifically into the chromosome. SXT excises from the chromosome to form a circular but nonreplicative extrachromosomal molecule that is required for its transfer. Here we found that a significant fraction of freshly isolated SXT exconjugants contained tandem SXT arrays. There was heterogeneity in the size of the SXT arrays detected in single exconjugant colonies. Some arrays consisted of more than five SXTs arranged in tan
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Haskett, Timothy L., Jason J. Terpolilli, Amanuel Bekuma, et al. "Assembly and transfer of tripartite integrative and conjugative genetic elements." Proceedings of the National Academy of Sciences 113, no. 43 (2016): 12268–73. http://dx.doi.org/10.1073/pnas.1613358113.

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Integrative and conjugative elements (ICEs) are ubiquitous mobile genetic elements present as “genomic islands” within bacterial chromosomes. Symbiosis islands are ICEs that convert nonsymbiotic mesorhizobia into symbionts of legumes. Here we report the discovery of symbiosis ICEs that exist as three separate chromosomal regions when integrated in their hosts, but through recombination assemble as a single circular ICE for conjugative transfer. Whole-genome comparisons revealed exconjugants derived from nonsymbiotic mesorhizobia received three separate chromosomal regions from the donorMesorhi
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Mingoia, Marina, Eleonora Morici, Gianluca Morroni, et al. "Tn5253Family Integrative and Conjugative Elements Carryingmef(I) andcatQDeterminants in Streptococcus pneumoniae and Streptococcus pyogenes." Antimicrobial Agents and Chemotherapy 58, no. 10 (2014): 5886–93. http://dx.doi.org/10.1128/aac.03638-14.

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ABSTRACTThe linkage between the macrolide efflux genemef(I) and the chloramphenicol inactivation genecatQwas first described inStreptococcus pneumoniae(strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected inStreptococcus pyogenes(strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253family integrative and conjugative elements (ICEs). The ICE fromS. pneumoniae(ICESpn529IQ) was sequenced, whereas the ICEs fromS. pyogenes(ICESpy
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Dimopoulou, Ioanna D., Sofia I. Kartali, Rosalind M. Harding, Tim E. A. Peto, and Derrick W. Crook. "Diversity of antibiotic resistance integrative and conjugative elements among haemophili." Journal of Medical Microbiology 56, no. 6 (2007): 838–46. http://dx.doi.org/10.1099/jmm.0.47125-0.

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The objective of this study was to investigate the sequence diversity in a single country of a family of integrative and conjugative elements (ICEs) that are vectors of antibiotic resistance in Haemophilus influenzae and Haemophilus parainfluenzae, and test the hypothesis that they emerged from a single lineage. Sixty subjects aged 9 months – 13 years were recruited and oropharyngeal samples cultured. Up to 10 morphologically distinct Pasteurellaceae spp. were purified, and then the species were determined and differentiated by partial sequence analysis of 16S rDNA and mdh, respectively. ICEs
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Haenni, Marisa, Estelle Saras, Stéphane Bertin, Pierre Leblond, Jean-Yves Madec, and Sophie Payot. "Diversity and Mobility of Integrative and Conjugative Elements in Bovine Isolates of Streptococcusagalactiae, S. dysgalactiae subsp. dysgalactiae, and S. uberis." Applied and Environmental Microbiology 76, no. 24 (2010): 7957–65. http://dx.doi.org/10.1128/aem.00805-10.

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ABSTRACT Bovine isolates of S treptococcus agalactiae (n = 76), S treptococcus dysgalactiae subsp. dysgalactiae (n = 32), and S treptococcus uberis (n = 101) were analyzed for the presence of different integrative and conjugative elements (ICEs) and their association with macrolide, lincosamide, and tetracycline resistance. The diversity of the isolates included in this study was demonstrated by multilocus sequence typing for S. agalactiae and pulsed-field gel electrophoresis for S. dysgalactiae and S. uberis. Most of the erythromycin-resistant strains carry an ermB gene. Five strains of S. ub
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Pavlovic, Guillaume, Vincent Burrus, Brigitte Gintz, Bernard Decaris, and Gérard Guédon. "Evolution of genomic islands by deletion and tandem accretion by site-specific recombination: ICESt1-related elements from Streptococcus thermophilus." Microbiology 150, no. 4 (2004): 759–74. http://dx.doi.org/10.1099/mic.0.26883-0.

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The 34 734-bp integrative and potentially conjugative element (putative ICE) ICESt1 has been previously found to be site-specifically integrated in the 3′ end of the fda locus of Streptococcus thermophilus CNRZ368. Four types of genomic islands related to ICESt1 are integrated in the same position in seven other strains of S. thermophilus. One of these elements, ICESt3, harbours conjugation and recombination modules closely related to those of ICESt1 and excises by site-specific recombination. Two other types of elements, CIME19258 and CIME302, are flanked by site-specific attachment sites clo
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Burrus, Vincent, Roberto Quezada-Calvillo, Joeli Marrero, and Matthew K. Waldor. "SXT-Related Integrating Conjugative Element in New World Vibrio cholerae." Applied and Environmental Microbiology 72, no. 4 (2006): 3054–57. http://dx.doi.org/10.1128/aem.72.4.3054-3057.2006.

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ABSTRACT SXT-related integrating conjugative elements (ICEs) became prevalent in Asian Vibrio cholerae populations after V. cholerae O139 emerged. Here, we describe an SXT-related ICE, ICEVchMex1, in a Mexican environmental V. cholerae isolate. Identification of ICEVchMex1 represents the first description of an SXT-related ICE in the Western Hemisphere. The significant differences between the SXT and ICEVchMex1 genomes suggest that these ICEs have evolved independently.
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Dangla-Pélissier, Gauthier, Nicolas Roux, Victoria Schmidt, et al. "The horizontal transfer of Pseudomonas aeruginosa PA14 ICE PAPI-1 is controlled by a transcriptional triad between TprA, NdpA2 and MvaT." Nucleic Acids Research 49, no. 19 (2021): 10956–74. http://dx.doi.org/10.1093/nar/gkab827.

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Abstract Pseudomonas aeruginosa is a major cause of nosocomial infections, particularly in immunocompromised patients or in individuals with cystic fibrosis. Genome sequences reveal that most P. aeruginosa strains contain a significant number of accessory genes gathered in genomic islands. Those genes are essential for P. aeruginosa to invade new ecological niches with high levels of antibiotic usage, like hospitals, or to survive during host infection by providing pathogenicity determinants. P. aeruginosa pathogenicity island 1 (PAPI-1), one of the largest genomic islands, encodes several put
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Bean, Emily L., Lisa K. McLellan, and Alan D. Grossman. "Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria." PLOS Genetics 18, no. 10 (2022): e1010467. http://dx.doi.org/10.1371/journal.pgen.1010467.

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Integrative and conjugative elements (ICEs) serve as major drivers of bacterial evolution. These elements often confer some benefit to host cells, including antibiotic resistance, metabolic capabilities, or pathogenic determinants. ICEs can also have negative effects on host cells. Here, we investigated the effects of the ICE (conjugative transposon) Tn916 on host cells. Because Tn916 is active in a relatively small subpopulation of host cells, we developed a fluorescent reporter system for monitoring activation of Tn916 in single cells. Using this reporter, we found that cell division was arr
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Eidam, C., A. Poehlein, A. Leimbach, et al. "Analysis and comparative genomics of ICEMh1, a novel integrative and conjugative element (ICE) of Mannheimia haemolytica." Journal of Antimicrobial Chemotherapy 70, no. 1 (2014): 93–97. http://dx.doi.org/10.1093/jac/dku361.

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34

Libante, Virginie, Yves Nombre, Charles Coluzzi, et al. "Chromosomal Conjugative and Mobilizable Elements in Streptococcus suis: Major Actors in the Spreading of Antimicrobial Resistance and Bacteriocin Synthesis Genes." Pathogens 9, no. 1 (2019): 22. http://dx.doi.org/10.3390/pathogens9010022.

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Streptococcus suis is a zoonotic pathogen suspected to be a reservoir of antimicrobial resistance (AMR) genes. The genomes of 214 strains of 27 serotypes were screened for AMR genes and chromosomal Mobile Genetic Elements (MGEs), in particular Integrative Conjugative Elements (ICEs) and Integrative Mobilizable Elements (IMEs). The functionality of two ICEs that host IMEs carrying AMR genes was investigated by excision tests and conjugation experiments. In silico search revealed 416 ICE-related and 457 IME-related elements. These MGEs exhibit an impressive diversity and plasticity with tandem a
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35

Marenda, Marc, Valérie Barbe, Géraldine Gourgues, Sophie Mangenot, Evelyne Sagne, and Christine Citti. "A New Integrative Conjugative Element Occurs in Mycoplasma agalactiae as Chromosomal and Free Circular Forms." Journal of Bacteriology 188, no. 11 (2006): 4137–41. http://dx.doi.org/10.1128/jb.00114-06.

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ABSTRACT An integrative conjugative element, ICEA, was characterized in Mycoplasma agalactiae strain 5632, in which it occurs as multiple chromosomal copies and as a free circular form. The distribution of ICEA sequences in M. agalactiae strains and their occurrence in Mycoplasma bovis suggest the spreading of the element within or between species.
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36

He, Dandan, Liangliang Wang, Shiyu Zhao, et al. "A novel tigecycline resistance gene, tet(X6), on an SXT/R391 integrative and conjugative element in a Proteus genomospecies 6 isolate of retail meat origin." Journal of Antimicrobial Chemotherapy 75, no. 5 (2020): 1159–64. http://dx.doi.org/10.1093/jac/dkaa012.

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Abstract Objectives To characterize a novel tigecycline resistance gene, tet(X6), and a novel SXT-related integrative and conjugative element (ICE), ICEPgs6Chn1, found in a tigecycline-resistant Proteus genomospecies 6 strain, T60. Methods Strain T60 was identified by the VITEK 2 system, biochemical reactions and an SNP-based approach. The genetic profile of strain T60 was determined by WGS analysis. ICEPgs6Chn1 was analysed by PCR, conjugation experiments and bioinformatics tools. tet(X6) was characterized by cloning and protein structure prediction. Results Strain T60 was resistant to ampici
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McLeod, Sarah M., Vincent Burrus, and Matthew K. Waldor. "Requirement for Vibrio cholerae Integration Host Factor in Conjugative DNA Transfer." Journal of Bacteriology 188, no. 16 (2006): 5704–11. http://dx.doi.org/10.1128/jb.00564-06.

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ABSTRACT The requirement for host factors in the transmission of integrative and conjugative elements (ICEs) has not been extensively explored. Here we tested whether integration host factor (IHF) or Fis, two host-encoded nucleoid proteins, are required for transfer of SXT, a Vibrio cholerae-derived ICE that can be transmitted to many gram-negative species. Fis did not influence the transfer of SXT to or from V. cholerae. In contrast, IHF proved to be required for V. cholerae to act as an SXT donor. In the absence of IHF, V. cholerae displayed a modest defect for serving as an SXT recipient. S
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Xu, Jinpeng, Haiyan Jia, Guanghui Cui, et al. "ICEAplChn1, a novel SXT/R391 integrative conjugative element (ICE), carrying multiple antibiotic resistance genes in Actinobacillus pleuropneumoniae." Veterinary Microbiology 220 (July 2018): 18–23. http://dx.doi.org/10.1016/j.vetmic.2018.05.002.

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39

Adabi, M., B. Bakhshi, H. Goudarzi, and M. R. Pourshafie. "Integrating Conjugative Element (ICE) among Environmental and Clinical Isolates of Vibrio cholerae." International Journal of Infectious Diseases 12 (December 2008): e119. http://dx.doi.org/10.1016/j.ijid.2008.05.297.

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40

Nonaka, Lisa, Michiaki Masuda, and Hirokazu Yano. "Atypical integrative element with strand-biased circularization activity assists interspecies antimicrobial resistance gene transfer from Vibrio alfacsensis." PLOS ONE 17, no. 8 (2022): e0271627. http://dx.doi.org/10.1371/journal.pone.0271627.

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The exchange of antimicrobial resistance (AMR) genes between aquaculture and terrestrial microbial populations has emerged as a serious public health concern. However, the nature of the mobile genetic elements in marine bacteria is poorly documented. To gain insight into the genetic mechanisms underlying AMR gene transfer from marine bacteria, we mated a multidrug-resistant Vibrio alfacsensis strain with an Escherichia coli strain, and then determined the complete genome sequences of the donor and the transconjugant strains. Sequence analysis revealed a conjugative multidrug resistance plasmid
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Kireeva, A. G., O. V. Kalinina, A. M. Kiselev, N. I. Briko, E. V. Glushkova, and A. V. Dmitriev. "AN OCCURRENCE OF ICE-emm12 GENETIC ELEMENT CONTAINING tetM AND ermB RESISTANCE GENES AMONG RUSSIAN AND VIETNAMESE GROUP A STREPTOCOCCAL STRAINS." Journal of microbiology epidemiology immunobiology, no. 2 (April 28, 2018): 23–30. http://dx.doi.org/10.36233/0372-9311-2018-2-23-30.

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Aim. Goal of the study is characterization and analysis of an occurrence of ICE-emm12 genetic element associated with streptococcal outbreaks, among Vietnamese and Russian GAS strains. Materials and methods. A total of 96 strains isolated in 2007 - 2014 in Moscow, Saint-Petersburg and different provinces of Vietnam were studied. Molecular genetic experiments were done as previously described. Whole genome sequencing was done using MiSeq technology. Results. Complete genome sequencing of Vietnamese strain V31 revealed the presence of 61028 bp fragment homologous to integrative and conjugative e
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Bean, Emily L., Janet L. Smith, and Alan D. Grossman. "Identification of insertion sites for the integrative and conjugative element Tn916 in the Bacillus subtilis chromosome." PLOS One 20, no. 5 (2025): e0318964. https://doi.org/10.1371/journal.pone.0318964.

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Integrative and conjugative elements (ICEs) are found in many bacterial species and are mediators of horizontal gene transfer. Tn916 is an ICE found in several Gram-positive genera, including Enterococcus, Staphylococcus, Streptococcus, and Clostridioides (previously Clostridium). In contrast to the many ICEs that preferentially integrate into a single site, Tn916 can integrate into many sites in the host chromosome. The consensus integration motif for Tn916, based on analyses of approximately 200 independent insertions, is an approximately 16 bp AT-rich sequence. Here, we describe the identif
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43

Abbott, Zachary D., Kaitlin J. Flynn, Brenda G. Byrne, Sampriti Mukherjee, Daniel B. Kearns, and Michele S. Swanson. "csrTRepresents a New Class ofcsrA-Like Regulatory Genes Associated with Integrative Conjugative Elements of Legionella pneumophila." Journal of Bacteriology 198, no. 3 (2015): 553–64. http://dx.doi.org/10.1128/jb.00732-15.

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ABSTRACTBacterial evolution is accelerated by mobile genetic elements. To spread horizontally and to benefit the recipient bacteria, genes encoded on these elements must be properly regulated. Among the legionellae are multiple integrative conjugative elements (ICEs) that each encode a paralog of the broadly conserved regulatorcsrA. Using bioinformatic analyses, we deduced that specificcsrAparalogs are coinherited with particular lineages of the type IV secretion system that mediates horizontal spread of its ICE, suggesting a conserved regulatory interaction. As a first step to investigate the
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44

Grim, Christopher J., Nur A. Hasan, Elisa Taviani, et al. "Genome Sequence of Hybrid Vibrio cholerae O1 MJ-1236, B-33, and CIRS101 and Comparative Genomics with V. cholerae." Journal of Bacteriology 192, no. 13 (2010): 3524–33. http://dx.doi.org/10.1128/jb.00040-10.

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ABSTRACT The genomes of V ibrio cholerae O1 Matlab variant MJ-1236, Mozambique O1 El Tor variant B33, and altered O1 El Tor CIRS101 were sequenced. All three strains were found to belong to the phylocore group 1 clade of V. cholerae, which includes the 7th-pandemic O1 El Tor and serogroup O139 isolates, despite displaying certain characteristics of the classical biotype. All three strains were found to harbor a hybrid variant of CTXΦ and an integrative conjugative element (ICE), leading to their establishment as successful clinical clones and the displacement of prototypical O1 El Tor. The abs
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45

Osorio, Carlos R., Joeli Marrero, Rachel A. F. Wozniak, Manuel L. Lemos, Vincent Burrus, and Matthew K. Waldor. "Genomic and Functional Analysis of ICEPdaSpa1, a Fish-Pathogen-Derived SXT-Related Integrating Conjugative Element That Can Mobilize a Virulence Plasmid." Journal of Bacteriology 190, no. 9 (2008): 3353–61. http://dx.doi.org/10.1128/jb.00109-08.

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ABSTRACT Integrating conjugative elements (ICEs) are self-transmissible mobile elements that transfer between bacteria via conjugation and integrate into the host chromosome. SXT and related ICEs became prevalent in Asian Vibrio cholerae populations in the 1990s and play an important role in the dissemination of antibiotic resistance genes in V. cholerae. Here, we carried out genomic and functional analyses of ICEPdaSpa1, an SXT-related ICE derived from a Spanish isolate of Photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis. The ∼102-kb DNA sequence of ICEPdaS
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Schubert, Sören, Severin Dufke, Johanna Sorsa, and Jürgen Heesemann. "A novel integrative and conjugative element (ICE) of Escherichia coli: the putative progenitor of the Yersinia high-pathogenicity island." Molecular Microbiology 51, no. 3 (2003): 837–48. http://dx.doi.org/10.1046/j.1365-2958.2003.03870.x.

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47

Baranowski, Eric, Emilie Dordet-Frisoni, Eveline Sagné, et al. "The Integrative Conjugative Element (ICE) ofMycoplasma agalactiae: Key Elements Involved in Horizontal Dissemination and Influence of Coresident ICEs." mBio 9, no. 4 (2018). http://dx.doi.org/10.1128/mbio.00873-18.

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ABSTRACTThe discovery of integrative conjugative elements (ICEs) in wall-less mycoplasmas and the demonstration of their role in massive gene flows within and across species have shed new light on the evolution of these minimal bacteria. Of these, the ICE of the ruminant pathogenMycoplasma agalactiae(ICEA) represents a prototype and belongs to a new clade of the Mutator-like superfamily that has no preferential insertion site and often occurs as multiple chromosomal copies. Here, functional genomics and mating experiments were combined to address ICEA functions and define the minimal ICEA chas
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48

Flynn, Kaitlin J., та Michele S. Swanson. "Integrative Conjugative Element ICE-βox Confers Oxidative Stress Resistance to Legionella pneumophila In Vitro and in Macrophages". mBio 5, № 3 (2014). http://dx.doi.org/10.1128/mbio.01091-14.

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ABSTRACTIntegrative conjugative elements (ICEs) are mobile blocks of DNA that can contribute to bacterial evolution by self-directed transmission of advantageous traits. Here, we analyze the activity of a putative 65-kb ICE harbored byLegionella pneumophilausing molecular genetics, conjugation assays, a phenotype microarray screen, and macrophage infections. The element transferred to a naiveL. pneumophilastrain, integrated site-specifically, and conferred increased resistance to oxacillin, penicillin, hydrogen peroxide, and bleach. Furthermore, the element increased survival ofL. pneumophilaw
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Lécuyer, Frédéric, Jean-Sébastien Bourassa, Martin Gélinas, Vincent Charron-Lamoureux, Vincent Burrus, and Pascale B. Beauregard. "Biofilm Formation Drives Transfer of the Conjugative Element ICEBs1inBacillus subtilis." mSphere 3, no. 5 (2018). http://dx.doi.org/10.1128/msphere.00473-18.

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ABSTRACTHorizontal gene transfer by integrative and conjugative elements (ICEs) is a very important mechanism for spreading antibiotic resistance in various bacterial species. In environmental and clinical settings, most bacteria form biofilms as a way to protect themselves against extracellular stress. However, much remains to be known about ICE transfer in biofilms. Using ICEBs1fromBacillus subtilis, we show that the natural conjugation efficiency of this ICE is greatly affected by the ability of the donor and recipient to form a biofilm. ICEBs1transfer considerably increases in biofilm, eve
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50

Colombi, Elena, Benjamin J. Perry, John T. Sullivan, et al. "Comparative analysis of integrative and conjugative mobile genetic elements in the genus Mesorhizobium." Microbial Genomics 7, no. 10 (2021). http://dx.doi.org/10.1099/mgen.0.000657.

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Members of the Mesorhizobium genus are soil bacteria that often form nitrogen-fixing symbioses with legumes. Most characterised Mesorhizobium spp. genomes are ~8 Mb in size and harbour extensive pangenomes including large integrative and conjugative elements (ICEs) carrying genes required for symbiosis (ICESyms). Here, we document and compare the conjugative mobilome of 41 complete Mesorhizobium genomes. We delineated 56 ICEs and 24 integrative and mobilizable elements (IMEs) collectively occupying 16 distinct integration sites, along with 24 plasmids. We also demonstrated horizontal transfer
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