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Journal articles on the topic 'Integrative Conjugative Elements (ICEs)'

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Published: 8 June 2024

Last updated: 31 July 2025

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1

Bellanger, Xavier, Adam P. Roberts, Catherine Morel, et al. "Conjugative Transfer of the Integrative Conjugative Elements ICESt1 and ICESt3 from Streptococcus thermophilus." Journal of Bacteriology 191, no. 8 (2009): 2764–75. http://dx.doi.org/10.1128/jb.01412-08.

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ABSTRACT Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus thermophilus, ICESt1 and ICESt3. Mitomycin C, a DNA-damaging agent, derepresses ICESt3 conjugative transfer almost 25-fold. The ICESt3 host range was determined using various members of the Firmicutes as recipients. Whereas numerous ICESt3 transconjugants of Streptococcus py

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Zakharova, I. B., and D. V. Viktorov. "Integrative conjugative elements (ICEs) of microorganisms." Molecular Genetics, Microbiology and Virology 30, no. 3 (2015): 114–23. http://dx.doi.org/10.3103/s0891416815030076.

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3

Iannelli, Francesco, Francesco Santoro, Marco R. Oggioni, and Gianni Pozzi. "Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253of Streptococcus pneumoniae." Antimicrobial Agents and Chemotherapy 58, no. 2 (2013): 1235–39. http://dx.doi.org/10.1128/aac.01764-13.

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ABSTRACTConjugative transposon Tn5253, an integrative conjugative element (ICE) ofStreptococcus pneumoniaecarrying thecatandtet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251(18,033 bp), of the Tn916-Tn1545family of ICEs, and Ωcat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253was observed whenSt

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4

Bioteau, Audrey, Romain Durand, and Vincent Burrus. "Redefinition and Unification of the SXT/R391 Family of Integrative and Conjugative Elements." Applied and Environmental Microbiology 84, no. 13 (2018): e00485-18. http://dx.doi.org/10.1128/aem.00485-18.

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ABSTRACT Integrative and conjugative elements (ICEs) of the SXT/R391 family are key drivers of the spread of antibiotic resistance in Vibrio cholerae, the infectious agent of cholera, and other pathogenic bacteria. The SXT/R391 family of ICEs was defined based on the conservation of a core set of 52 genes and site-specific integration into the 5′ end of the chromosomal gene prfC. Hence, the integrase gene int has been intensively used as a marker to detect SXT/R391 ICEs in clinical isolates. ICEs sharing most core genes but differing by their integration site and integrase gene have been recen

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Lei, Chang-Wei, An-Yun Zhang, Hong-Ning Wang, Bi-Hui Liu, Li-Qin Yang, and Yong-Qiang Yang. "Characterization of SXT/R391 Integrative and Conjugative Elements in Proteus mirabilis Isolates from Food-Producing Animals in China." Antimicrobial Agents and Chemotherapy 60, no. 3 (2016): 1935–38. http://dx.doi.org/10.1128/aac.02852-15.

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SXT/R391 integrative and conjugative elements (ICEs) were detected in 8 out of 125Proteus mirabilisisolates from food-producing animals in China. Whole-genome sequencing revealed that seven ICEs were identical to ICEPmiJpn1, carrying the cephalosporinase geneblaCMY-2. Another one, designated ICEPmiChn1, carried five resistance genes. All eight ICEs could be transferred toEscherichia colivia conjugation. The results highlight the idea that animal farms are important reservoir of the SXT/R391 ICE-containingP. mirabilis.

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6

Libante, Virginie, Yves Nombre, Charles Coluzzi, et al. "Chromosomal Conjugative and Mobilizable Elements in Streptococcus suis: Major Actors in the Spreading of Antimicrobial Resistance and Bacteriocin Synthesis Genes." Pathogens 9, no. 1 (2019): 22. http://dx.doi.org/10.3390/pathogens9010022.

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Streptococcus suis is a zoonotic pathogen suspected to be a reservoir of antimicrobial resistance (AMR) genes. The genomes of 214 strains of 27 serotypes were screened for AMR genes and chromosomal Mobile Genetic Elements (MGEs), in particular Integrative Conjugative Elements (ICEs) and Integrative Mobilizable Elements (IMEs). The functionality of two ICEs that host IMEs carrying AMR genes was investigated by excision tests and conjugation experiments. In silico search revealed 416 ICE-related and 457 IME-related elements. These MGEs exhibit an impressive diversity and plasticity with tandem a

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7

Haskett, Timothy L., Jason J. Terpolilli, Amanuel Bekuma, et al. "Assembly and transfer of tripartite integrative and conjugative genetic elements." Proceedings of the National Academy of Sciences 113, no. 43 (2016): 12268–73. http://dx.doi.org/10.1073/pnas.1613358113.

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Integrative and conjugative elements (ICEs) are ubiquitous mobile genetic elements present as “genomic islands” within bacterial chromosomes. Symbiosis islands are ICEs that convert nonsymbiotic mesorhizobia into symbionts of legumes. Here we report the discovery of symbiosis ICEs that exist as three separate chromosomal regions when integrated in their hosts, but through recombination assemble as a single circular ICE for conjugative transfer. Whole-genome comparisons revealed exconjugants derived from nonsymbiotic mesorhizobia received three separate chromosomal regions from the donorMesorhi

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8

Hirose, Jun. "Diversity and Evolution of Integrative and Conjugative Elements Involved in Bacterial Aromatic Compound Degradation and Their Utility in Environmental Remediation." Microorganisms 11, no. 2 (2023): 438. http://dx.doi.org/10.3390/microorganisms11020438.

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Integrative and conjugative elements (ICEs) are mobile DNA molecules that can be transferred through excision, conjugation, and integration into chromosomes. They contribute to the horizontal transfer of genomic islands across bacterial species. ICEs carrying genes encoding aromatic compound degradation pathways are of interest because of their contribution to environmental remediation. Recent advances in DNA sequencing technology have increased the number of newly discovered ICEs in bacterial genomes and have enabled comparative analysis of their evolution. The two different families of ICEs

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9

Iannelli, Francesco, Francesco Santoro, Valeria Fox, and Gianni Pozzi. "A Mating Procedure for Genetic Transfer of Integrative and Conjugative Elements (ICEs) of Streptococci and Enterococci." Methods and Protocols 4, no. 3 (2021): 59. http://dx.doi.org/10.3390/mps4030059.

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DNA sequencing of whole bacterial genomes has revealed that the entire set of mobile genes (mobilome) represents as much as 25% of the bacterial genome. Despite the huge availability of sequence data, the functional analysis of the mobile genetic elements (MGEs) is rarely reported. Therefore, established laboratory protocols are needed to investigate the biology of this important part of the bacterial genome. Conjugation is a mechanism of horizontal gene transfer which allows the exchange of MGEs among strains of the same or different bacterial species. In streptococci and enterococci, integra

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Dimopoulou, Ioanna D., Sofia I. Kartali, Rosalind M. Harding, Tim E. A. Peto, and Derrick W. Crook. "Diversity of antibiotic resistance integrative and conjugative elements among haemophili." Journal of Medical Microbiology 56, no. 6 (2007): 838–46. http://dx.doi.org/10.1099/jmm.0.47125-0.

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The objective of this study was to investigate the sequence diversity in a single country of a family of integrative and conjugative elements (ICEs) that are vectors of antibiotic resistance in Haemophilus influenzae and Haemophilus parainfluenzae, and test the hypothesis that they emerged from a single lineage. Sixty subjects aged 9 months – 13 years were recruited and oropharyngeal samples cultured. Up to 10 morphologically distinct Pasteurellaceae spp. were purified, and then the species were determined and differentiated by partial sequence analysis of 16S rDNA and mdh, respectively. ICEs

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11

Mingoia, Marina, Eleonora Morici, Gianluca Morroni, et al. "Tn5253Family Integrative and Conjugative Elements Carryingmef(I) andcatQDeterminants in Streptococcus pneumoniae and Streptococcus pyogenes." Antimicrobial Agents and Chemotherapy 58, no. 10 (2014): 5886–93. http://dx.doi.org/10.1128/aac.03638-14.

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ABSTRACTThe linkage between the macrolide efflux genemef(I) and the chloramphenicol inactivation genecatQwas first described inStreptococcus pneumoniae(strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected inStreptococcus pyogenes(strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253family integrative and conjugative elements (ICEs). The ICE fromS. pneumoniae(ICESpn529IQ) was sequenced, whereas the ICEs fromS. pyogenes(ICESpy

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Ringwald, Kenneth, and Jeffrey Gardner. "The Bacteroides thetaiotaomicron Protein Bacteroides Host Factor A Participates in Integration of the Integrative Conjugative Element CTnDOT into the Chromosome." Journal of Bacteriology 197, no. 8 (2015): 1339–49. http://dx.doi.org/10.1128/jb.02198-14.

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ABSTRACTCTnDOT is a conjugative transposon found inBacteroidesspecies. It encodes multiple antibiotic resistances and is stimulated to transfer by exposure to tetracycline. CTnDOT integration into the host chromosome requires IntDOT and a previously unknown host factor. We have identified a protein, designated BHFa (Bacteroideshost factor A), that participates in integrative recombination. BHFa is the first host factor identified for a site-specific recombination reaction in the CTnDOT family of integrative and conjugative elements. Based on the amino acid sequence of BHFa, the ability to bind

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Lao, Julie, Gérard Guédon, Thomas Lacroix, et al. "Abundance, Diversity and Role of ICEs and IMEs in the Adaptation of Streptococcus salivarius to the Environment." Genes 11, no. 9 (2020): 999. http://dx.doi.org/10.3390/genes11090999.

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Streptococcus salivarius is a significant contributor to the human oral, pharyngeal and gut microbiomes that contribute to the maintenance of health. The high genomic diversity observed in this species is mainly caused by horizontal gene transfer. This work aimed to evaluate the contribution of integrative and conjugative elements (ICEs) and integrative and mobilizable elements (IMEs) in S. salivarius genome diversity. For this purpose, we performed an in-depth analysis of 75 genomes of S. salivarius and searched for signature genes of conjugative and mobilizable elements. This analysis led to

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Zakharova, Irina B., Yu A. Kuzyutina, M. V. Podshivalova, A. A. Zamarin, A. V. Toporkov, and D. V. Viktorov. "Detection and analysis of integrative conjugative elements in Vibrio spp. strains, isolated in the Volgograd region." Epidemiology and Infectious Diseases 21, no. 6 (2016): 347–51. http://dx.doi.org/10.17816/eid40944.

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The presence of integrative conjugative elements (ICEs) of SXT/R391 family in different Vibrio species isolated from natural water sources in the Volgograd region in 2003 - 2014 was established. Trimethoprim (dfr18), streptomycin (strB) and sulfametoxazol (sulII) resistance genes were detected in ICEs pattern of in V. choleraenon-O1/non-O139 strains. In Vibrio spp. ICEs strains not referred to V. cholerae type there was detected another variant of dihydrofolate reductase gene - dfrA1 localized outside the main resistance cluster. The obtained results indicate to that aqueous Vibrio spp. strain

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Tardy, Florence, Virginie Mick, Emilie Dordet-Frisoni, et al. "Integrative Conjugative Elements Are Widespread in Field Isolates of Mycoplasma Species Pathogenic for Ruminants." Applied and Environmental Microbiology 81, no. 5 (2014): 1634–43. http://dx.doi.org/10.1128/aem.03723-14.

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ABSTRACTComparative genomics have revealed massive horizontal gene transfer (HGT) betweenMycoplasmaspecies sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogenMycoplasma agalactiae. To estimate the prevalence of ICEs in ruminant mycoplasmas, we surveyed their occurrence in a collection of 166 field strains representing 4 (sub)species that are recognized as major pathogens. Based on available sequenced genomes, we first defined the conserved, minimal ICE backbone as composed of 4

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McKeithen-Mead, Saria A., and Alan D. Grossman. "Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host." PLOS Genetics 19, no. 2 (2023): e1010524. http://dx.doi.org/10.1371/journal.pgen.1010524.

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Integrative and conjugative elements (ICEs) are major contributors to genome plasticity in bacteria. ICEs reside integrated in the chromosome of a host bacterium and are passively propagated during chromosome replication and cell division. When activated, ICEs excise from the chromosome and may be transferred through the ICE-encoded conjugation machinery into a recipient cell. Integration into the chromosome of the new host generates a stable transconjugant. Although integration into the chromosome of a new host is critical for the stable acquisition of ICEs, few studies have directly investig

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LeGault, Kristen N., Stephanie G. Hays, Angus Angermeyer, et al. "Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts." Science 373, no. 6554 (2021): eabg2166. http://dx.doi.org/10.1126/science.abg2166.

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Bacteriophage predation selects for diverse antiphage systems that frequently cluster on mobilizable defense islands in bacterial genomes. However, molecular insight into the reciprocal dynamics of phage-bacterial adaptations in nature is lacking, particularly in clinical contexts where there is need to inform phage therapy efforts and to understand how phages drive pathogen evolution. Using time-shift experiments, we uncovered fluctuations in Vibrio cholerae’s resistance to phages in clinical samples. We mapped phage resistance determinants to SXT integrative and conjugative elements (ICEs),

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Wirachman, Erika S., and Alan D. Grossman. "Transcription termination and antitermination are critical for the fitness and function of the integrative and conjugative element Tn916." PLOS Genetics 20, no. 12 (2024): e1011417. https://doi.org/10.1371/journal.pgen.1011417.

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Premature expression of genes in mobile genetic elements can be detrimental to their bacterial hosts. Tn916, the founding member of a large family of integrative and conjugative elements (ICEs; aka conjugative transposons), confers tetracycline-resistance and is found in several Gram-positive bacterial species. We identified a transcription terminator near one end of Tn916 that functions as an insulator that prevents expression of element genes when Tn916 is integrated downstream from an active host promoter. The terminator blocked expression of Tn916 genes needed for unwinding and rolling cir

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19

Shang, Yanhong, Dexi Li, Wenbo Hao, et al. "A prophage and two ICESa2603-family integrative and conjugative elements (ICEs) carrying optrA in Streptococcus suis." Journal of Antimicrobial Chemotherapy 74, no. 10 (2019): 2876–79. http://dx.doi.org/10.1093/jac/dkz309.

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Abstract Objectives To investigate the presence and transfer of the oxazolidinone/phenicol resistance gene optrA and identify the genetic elements involved in the horizontal transfer of the optrA gene in Streptococcus suis. Methods A total of 237 S. suis isolates were screened for the presence of the optrA gene by PCR. Whole-genome DNA of three optrA-positive strains was completely sequenced using the Illumina MiSeq and Pacbio RSII platforms. MICs were determined by broth microdilution. Transferability of the optrA gene in S. suis was investigated by conjugation. The presence of circular inter

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Lee, Catherine A., and Alan D. Grossman. "Identification of the Origin of Transfer (oriT) and DNA Relaxase Required for Conjugation of the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis." Journal of Bacteriology 189, no. 20 (2007): 7254–61. http://dx.doi.org/10.1128/jb.00932-07.

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ABSTRACT Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide signaling system. When B. subtilis senses DNA damage or high concentrations of potential mating partners that lack the element, ICEBs1 excises from the chromosome and can transfer to recipients. Bacterial conjugation usually requires a DNA relaxase that nicks an origin o

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Johnson, Christopher M., and Alan D. Grossman. "Integrative and Conjugative Elements (ICEs): What They Do and How They Work." Annual Review of Genetics 49, no. 1 (2015): 577–601. http://dx.doi.org/10.1146/annurev-genet-112414-055018.

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Bean, Emily L., Lisa K. McLellan, and Alan D. Grossman. "Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria." PLOS Genetics 18, no. 10 (2022): e1010467. http://dx.doi.org/10.1371/journal.pgen.1010467.

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Integrative and conjugative elements (ICEs) serve as major drivers of bacterial evolution. These elements often confer some benefit to host cells, including antibiotic resistance, metabolic capabilities, or pathogenic determinants. ICEs can also have negative effects on host cells. Here, we investigated the effects of the ICE (conjugative transposon) Tn916 on host cells. Because Tn916 is active in a relatively small subpopulation of host cells, we developed a fluorescent reporter system for monitoring activation of Tn916 in single cells. Using this reporter, we found that cell division was arr

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Bean, Emily L., Calvin Herman, Mary E. Anderson, and Alan D. Grossman. "Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies." PLOS Genetics 18, no. 5 (2022): e1009998. http://dx.doi.org/10.1371/journal.pgen.1009998.

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Integrative and conjugative elements (ICEs) are mobile genetic elements that reside in a bacterial host chromosome and are prominent drivers of bacterial evolution. They are also powerful tools for genetic analyses and engineering. Transfer of an ICE to a new host involves many steps, including excision from the chromosome, DNA processing and replication, transfer across the envelope of the donor and recipient, processing of the DNA, and eventual integration into the chromosome of the new host (now a stable transconjugant). Interactions between an ICE and its host throughout the life cycle lik

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Abbott, Zachary D., Kaitlin J. Flynn, Brenda G. Byrne, Sampriti Mukherjee, Daniel B. Kearns, and Michele S. Swanson. "csrTRepresents a New Class ofcsrA-Like Regulatory Genes Associated with Integrative Conjugative Elements of Legionella pneumophila." Journal of Bacteriology 198, no. 3 (2015): 553–64. http://dx.doi.org/10.1128/jb.00732-15.

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ABSTRACTBacterial evolution is accelerated by mobile genetic elements. To spread horizontally and to benefit the recipient bacteria, genes encoded on these elements must be properly regulated. Among the legionellae are multiple integrative conjugative elements (ICEs) that each encode a paralog of the broadly conserved regulatorcsrA. Using bioinformatic analyses, we deduced that specificcsrAparalogs are coinherited with particular lineages of the type IV secretion system that mediates horizontal spread of its ICE, suggesting a conserved regulatory interaction. As a first step to investigate the

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Libante, Virginie, Nazim Sarica, Abbas Mohamad Ali, et al. "Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins." Genes 11, no. 9 (2020): 1004. http://dx.doi.org/10.3390/genes11091004.

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Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are in

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Burrus, Vincent, Roberto Quezada-Calvillo, Joeli Marrero, and Matthew K. Waldor. "SXT-Related Integrating Conjugative Element in New World Vibrio cholerae." Applied and Environmental Microbiology 72, no. 4 (2006): 3054–57. http://dx.doi.org/10.1128/aem.72.4.3054-3057.2006.

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ABSTRACT SXT-related integrating conjugative elements (ICEs) became prevalent in Asian Vibrio cholerae populations after V. cholerae O139 emerged. Here, we describe an SXT-related ICE, ICEVchMex1, in a Mexican environmental V. cholerae isolate. Identification of ICEVchMex1 represents the first description of an SXT-related ICE in the Western Hemisphere. The significant differences between the SXT and ICEVchMex1 genomes suggest that these ICEs have evolved independently.

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Harada, Sohei, Yoshikazu Ishii, Tomoo Saga, Kazuhiro Tateda, and Keizo Yamaguchi. "Chromosomally Encoded blaCMY-2 Located on a Novel SXT/R391-Related Integrating Conjugative Element in a Proteus mirabilis Clinical Isolate." Antimicrobial Agents and Chemotherapy 54, no. 9 (2010): 3545–50. http://dx.doi.org/10.1128/aac.00111-10.

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ABSTRACT Integrating conjugative elements (ICEs) are mobile genetic elements that can transfer from the chromosome of a host to the chromosome of a new host through the process of excision, conjugation, and integration. Although SXT/R391-related ICEs, originally demonstrated in Vibrio cholerae O139 isolates, have become prevalent among V. cholerae isolates in Asia, the prevalence of the ICEs among Gram-negative bacteria other than Vibrio spp. remains unknown. In addition, SXT/R391-related ICEs carrying genes conferring resistance to extended-spectrum cephalosporins have never been described. H

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Haenni, Marisa, Estelle Saras, Stéphane Bertin, Pierre Leblond, Jean-Yves Madec, and Sophie Payot. "Diversity and Mobility of Integrative and Conjugative Elements in Bovine Isolates of Streptococcusagalactiae, S. dysgalactiae subsp. dysgalactiae, and S. uberis." Applied and Environmental Microbiology 76, no. 24 (2010): 7957–65. http://dx.doi.org/10.1128/aem.00805-10.

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ABSTRACT Bovine isolates of S treptococcus agalactiae (n = 76), S treptococcus dysgalactiae subsp. dysgalactiae (n = 32), and S treptococcus uberis (n = 101) were analyzed for the presence of different integrative and conjugative elements (ICEs) and their association with macrolide, lincosamide, and tetracycline resistance. The diversity of the isolates included in this study was demonstrated by multilocus sequence typing for S. agalactiae and pulsed-field gel electrophoresis for S. dysgalactiae and S. uberis. Most of the erythromycin-resistant strains carry an ermB gene. Five strains of S. ub

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Michaelis, Claudia, and Elisabeth Grohmann. "Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms." Antibiotics 12, no. 2 (2023): 328. http://dx.doi.org/10.3390/antibiotics12020328.

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Most bacteria attach to biotic or abiotic surfaces and are embedded in a complex matrix which is known as biofilm. Biofilm formation is especially worrisome in clinical settings as it hinders the treatment of infections with antibiotics due to the facilitated acquisition of antibiotic resistance genes (ARGs). Environmental settings are now considered as pivotal for driving biofilm formation, biofilm-mediated antibiotic resistance development and dissemination. Several studies have demonstrated that environmental biofilms can be hotspots for the dissemination of ARGs. These genes can be encoded

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Johnson, Christopher M., M. Michael Harden, and Alan D. Grossman. "Interactions between mobile genetic elements: An anti-phage gene in an integrative and conjugative element protects host cells from predation by a temperate bacteriophage." PLOS Genetics 18, no. 2 (2022): e1010065. http://dx.doi.org/10.1371/journal.pgen.1010065.

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Most bacterial genomes contain horizontally acquired and transmissible mobile genetic elements, including temperate bacteriophages and integrative and conjugative elements. Little is known about how these elements interact and co-evolved as parts of their host genomes. In many cases, it is not known what advantages, if any, these elements provide to their bacterial hosts. Most strains of Bacillus subtilis contain the temperate phage SPß and the integrative and conjugative element ICEBs1. Here we show that the presence of ICEBs1 in cells protects populations of B. subtilis from predation by SPß

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McLeod, Sarah M., Vincent Burrus, and Matthew K. Waldor. "Requirement for Vibrio cholerae Integration Host Factor in Conjugative DNA Transfer." Journal of Bacteriology 188, no. 16 (2006): 5704–11. http://dx.doi.org/10.1128/jb.00564-06.

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ABSTRACT The requirement for host factors in the transmission of integrative and conjugative elements (ICEs) has not been extensively explored. Here we tested whether integration host factor (IHF) or Fis, two host-encoded nucleoid proteins, are required for transfer of SXT, a Vibrio cholerae-derived ICE that can be transmitted to many gram-negative species. Fis did not influence the transfer of SXT to or from V. cholerae. In contrast, IHF proved to be required for V. cholerae to act as an SXT donor. In the absence of IHF, V. cholerae displayed a modest defect for serving as an SXT recipient. S

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Botelho, João, Filipa Grosso, and Luísa Peixe. "ICEs Are the Main Reservoirs of the Ciprofloxacin-Modifying crpP Gene in Pseudomonas aeruginosa." Genes 11, no. 8 (2020): 889. http://dx.doi.org/10.3390/genes11080889.

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The ciprofloxacin-modifying crpP gene was recently identified in a plasmid isolated from a Pseudomonas aeruginosa clinical isolate. Homologues of this gene were also identified in Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii. We set out to explore the mobile elements involved in the acquisition and spread of this gene in publicly available and complete genomes of Pseudomonas spp. All Pseudomonas complete genomes were downloaded from NCBI’s Refseq library and were inspected for the presence of the crpP gene. The mobile elements carrying this gene were further characterize

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Hirose, Jun, Takahito Watanabe, Taiki Futagami, et al. "A New ICEclc Subfamily Integrative and Conjugative Element Responsible for Horizontal Transfer of Biphenyl and Salicylic Acid Catabolic Pathway in the PCB-Degrading Strain Pseudomonas stutzeri KF716." Microorganisms 9, no. 12 (2021): 2462. http://dx.doi.org/10.3390/microorganisms9122462.

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Integrative and conjugative elements (ICEs) are chromosomally integrated self-transmissible mobile genetic elements. Although some ICEs are known to carry genes for the degradation of aromatic compounds, information on their genetic features is limited. We identified a new member of the ICEclc family carrying biphenyl catabolic bph genes and salicylic acid catabolic sal genes from the PCB-degrading strain Pseudomonas stutzeri KF716. The 117-kb ICEbph-salKF716 contains common core regions exhibiting homology with those of degradative ICEclc from P. knackmussii B13 and ICEXTD from Azoarcus sp. C

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Carraro, Nicolas, Dominique Poulin, and Vincent Burrus. "Replication and Active Partition of Integrative and Conjugative Elements (ICEs) of the SXT/R391 Family: The Line between ICEs and Conjugative Plasmids Is Getting Thinner." PLOS Genetics 11, no. 6 (2015): e1005298. http://dx.doi.org/10.1371/journal.pgen.1005298.

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Lamichhane, Jyoti, Brian I. Choi, Natalie Stegman, Melline Fontes Noronha, and Alan J. Wolfe. "Macrolide Resistance in the Aerococcus urinae Complex: Implications for Integrative and Conjugative Elements." Antibiotics 13, no. 5 (2024): 433. http://dx.doi.org/10.3390/antibiotics13050433.

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The recognition of the Aerococcus urinae complex (AUC) as an emerging uropathogen has led to growing concerns due to a limited understanding of its disease spectrum and antibiotic resistance profiles. Here, we investigated the prevalence of macrolide resistance within urinary AUC isolates, shedding light on potential genetic mechanisms. Phenotypic testing revealed a high rate of macrolide resistance: 45%, among a total of 189 urinary AUC isolates. Genomic analysis identified integrative and conjugative elements (ICEs) as carriers of the macrolide resistance gene ermA, suggesting horizontal gen

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Rodríguez-Blanco, Arturo, Manuel L. Lemos, and Carlos R. Osorio. "Integrating Conjugative Elements as Vectors of Antibiotic, Mercury, and Quaternary Ammonium Compound Resistance in Marine Aquaculture Environments." Antimicrobial Agents and Chemotherapy 56, no. 5 (2012): 2619–26. http://dx.doi.org/10.1128/aac.05997-11.

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ABSTRACTThe presence of SXT/R391-related integrating conjugative elements (ICEs) in bacterial strains isolated from fish obtained from marine aquaculture environments in 2001 to 2010 in the northwestern Iberian Peninsula was studied. ICEs were detected in 12 strains taxonomically related toVibrio scophthalmi(3 strains),Vibrio splendidus(5 strains),Vibrio alginolyticus(1 strain),Shewanella haliotis(1 strain), andEnterovibrio nigricans(2 strains), broadening the known host range able to harbor SXT/R391-like ICEs. Variable DNA regions, which confer element-specific properties to ICEs of this fami

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López de Egea, Guillem, Aida González-Díaz, Gérard Guédon, et al. "A New Integrative and Mobilizable Element Is a Major Contributor to Tetracycline Resistance in Streptococcus dysgalactiae subsp. equisimilis." Antibiotics 12, no. 3 (2023): 579. http://dx.doi.org/10.3390/antibiotics12030579.

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Tetracycline resistance in streptococci is mainly due to ribosomal protection mediated by the tet(M) gene that is usually located in the integrative and conjugative elements (ICEs) of the Tn916-family. In this study, we analyzed the genes involved in tetracycline resistance and the associated mobile genetic elements (MGEs) in Streptococcus dysgalactiae subsp. equisimilis (SDSE) causing invasive disease. SDSE resistant to tetracycline collected from 2012 to 2019 in a single hospital and from 2018 in three other hospitals were analyzed by whole genome sequencing. Out of a total of 84 SDSE isolat

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Burrus, Vincent, and Matthew K. Waldor. "Formation of SXT Tandem Arrays and SXT-R391 Hybrids." Journal of Bacteriology 186, no. 9 (2004): 2636–45. http://dx.doi.org/10.1128/jb.186.9.2636-2645.2004.

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ABSTRACT SXT is an integrative and conjugative element (ICE) isolated from Vibrio cholerae. This ∼100-kb ICE encodes resistance to multiple antibiotics and integrates site specifically into the chromosome. SXT excises from the chromosome to form a circular but nonreplicative extrachromosomal molecule that is required for its transfer. Here we found that a significant fraction of freshly isolated SXT exconjugants contained tandem SXT arrays. There was heterogeneity in the size of the SXT arrays detected in single exconjugant colonies. Some arrays consisted of more than five SXTs arranged in tan

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Osorio, Carlos R., Joeli Marrero, Rachel A. F. Wozniak, Manuel L. Lemos, Vincent Burrus, and Matthew K. Waldor. "Genomic and Functional Analysis of ICEPdaSpa1, a Fish-Pathogen-Derived SXT-Related Integrating Conjugative Element That Can Mobilize a Virulence Plasmid." Journal of Bacteriology 190, no. 9 (2008): 3353–61. http://dx.doi.org/10.1128/jb.00109-08.

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ABSTRACT Integrating conjugative elements (ICEs) are self-transmissible mobile elements that transfer between bacteria via conjugation and integrate into the host chromosome. SXT and related ICEs became prevalent in Asian Vibrio cholerae populations in the 1990s and play an important role in the dissemination of antibiotic resistance genes in V. cholerae. Here, we carried out genomic and functional analyses of ICEPdaSpa1, an SXT-related ICE derived from a Spanish isolate of Photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis. The ∼102-kb DNA sequence of ICEPdaS

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Dec, Marta, Aldert Zomer, John Webster, Tomasz Nowak, Dagmara Stępień-Pyśniak, and Renata Urban-Chmiel. "Integrative and Conjugative Elements and Prophage DNA as Carriers of Resistance Genes in Erysipelothrix rhusiopathiae Strains from Domestic Geese in Poland." International Journal of Molecular Sciences 25, no. 9 (2024): 4638. http://dx.doi.org/10.3390/ijms25094638.

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Goose erysipelas is a serious problem in waterfowl breeding in Poland. However, knowledge of the characteristics of Erysipelothrix rhusiopathiae strains causing this disease is limited. In this study, the antimicrobial susceptibility and serotypes of four E. rhusiopathiae strains from domestic geese were determined, and their whole-genome sequences (WGSs) were analyzed to detect resistance genes, integrative and conjugative elements (ICEs), and prophage DNA. Sequence type and the presence of resistance genes and transposons were compared with 363 publicly available E. rhusiopathiae strains, as

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Carraro, Nicolas, Vladimir Sentchilo, Lenka Polák, Claire Bertelli, and Jan Roelof van der Meer. "Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1." Genes 11, no. 8 (2020): 930. http://dx.doi.org/10.3390/genes11080930.

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The sewage sludge isolate Pseudomonas nitroreducens HBP-1 was the first bacterium known to completely degrade the fungicide 2-hydroxybiphenyl. PacBio and Illumina whole-genome sequencing revealed three circular DNA replicons: a chromosome and two plasmids. Plasmids were shown to code for putative adaptive functions such as heavy metal resistance, but with unclarified ability for self-transfer. About one-tenth of strain HBP-1′s chromosomal genes are likely of recent horizontal influx, being part of genomic islands, prophages and integrative and conjugative elements (ICEs). P. nitroreducens carr

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Ceccarelli, Daniela, Aurélie Daccord, Mélissa René, and Vincent Burrus. "Identification of the Origin of Transfer (oriT) and a New Gene Required for Mobilization of the SXT/R391 Family of Integrating Conjugative Elements." Journal of Bacteriology 190, no. 15 (2008): 5328–38. http://dx.doi.org/10.1128/jb.00150-08.

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ABSTRACT Integrating conjugative elements (ICEs) are self-transmissible, mobile elements that are widespread among bacteria. Following their excision from the chromosome, ICEs transfer by conjugation, a process initiated by a single-stranded DNA break at a specific locus called the origin of transfer (oriT). The SXT/R391 family of ICEs includes SXTMO10, R391, and more than 25 related ICEs found in gammaproteobacteria. A previous study mapped the oriT locus of SXTMO10 to a 550-bp intergenic region between traD and s043. We suspected that this was not the correct oriT locus, because the identica

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López, María, Beatriz Rojo-Bezares, Gabriela Chichón, and Yolanda Sáenz. "Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs." Antibiotics 11, no. 9 (2022): 1271. http://dx.doi.org/10.3390/antibiotics11091271.

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Fluoroquinolone resistance and the associated genetic mechanisms were assessed by antimicrobial susceptibility and whole genome sequencing in 56 Pseudomonas aeruginosa strains from human, animal, food and environmental origins. P. aeruginosa PAO1, PA7 and PA14 reference strains were also included in the study. Twenty-two strains (37%) were resistant to, at least, one fluoroquinolone agent. Correlation between the number of changes in GyrA and ParC proteins and the level of fluoroquinolone resistance was observed. Mutations or absence of genes, such as mexZ, mvaT and nalD encoding efflux pumps

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Bean, Emily L., Janet L. Smith, and Alan D. Grossman. "Identification of insertion sites for the integrative and conjugative element Tn916 in the Bacillus subtilis chromosome." PLOS One 20, no. 5 (2025): e0318964. https://doi.org/10.1371/journal.pone.0318964.

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Integrative and conjugative elements (ICEs) are found in many bacterial species and are mediators of horizontal gene transfer. Tn916 is an ICE found in several Gram-positive genera, including Enterococcus, Staphylococcus, Streptococcus, and Clostridioides (previously Clostridium). In contrast to the many ICEs that preferentially integrate into a single site, Tn916 can integrate into many sites in the host chromosome. The consensus integration motif for Tn916, based on analyses of approximately 200 independent insertions, is an approximately 16 bp AT-rich sequence. Here, we describe the identif

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Veschetti, Laura, Angela Sandri, Cristina Patuzzo, Paola Melotti, Giovanni Malerba, and Maria M. Lleò. "Mobilome Analysis of Achromobacter spp. Isolates from Chronic and Occasional Lung Infection in Cystic Fibrosis Patients." Microorganisms 9, no. 1 (2021): 130. http://dx.doi.org/10.3390/microorganisms9010130.

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Achromobacter spp. is an opportunistic pathogen that can cause lung infections in patients with cystic fibrosis (CF). Although a variety of mobile genetic elements (MGEs) carrying antimicrobial resistance genes have been identified in clinical isolates, little is known about the contribution of Achromobacter spp. mobilome to its pathogenicity. To provide new insights, we performed bioinformatic analyses of 54 whole genome sequences and investigated the presence of phages, insertion sequences (ISs), and integrative and conjugative elements (ICEs). Most of the detected phages were previously des

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Veschetti, Laura, Angela Sandri, Cristina Patuzzo, Paola Melotti, Giovanni Malerba, and Maria M. Lleò. "Mobilome Analysis of Achromobacter spp. Isolates from Chronic and Occasional Lung Infection in Cystic Fibrosis Patients." Microorganisms 9, no. 1 (2021): 130. http://dx.doi.org/10.3390/microorganisms9010130.

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Achromobacter spp. is an opportunistic pathogen that can cause lung infections in patients with cystic fibrosis (CF). Although a variety of mobile genetic elements (MGEs) carrying antimicrobial resistance genes have been identified in clinical isolates, little is known about the contribution of Achromobacter spp. mobilome to its pathogenicity. To provide new insights, we performed bioinformatic analyses of 54 whole genome sequences and investigated the presence of phages, insertion sequences (ISs), and integrative and conjugative elements (ICEs). Most of the detected phages were previously des

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Lorenzo-Diaz, Fabian, Cris Fernández-Lopez, Pierre-Emmanuel Douarre, et al. "Streptococcal group B integrative and mobilizable element IMESag- rpsI encodes a functional relaxase involved in its transfer." Open Biology 6, no. 10 (2016): 160084. http://dx.doi.org/10.1098/rsob.160084.

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Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. A

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Khan, Uzma Basit, Edward A. R. Portal, Kirsty Sands, et al. "Genomic Analysis Reveals New Integrative Conjugal Elements and Transposons in GBS Conferring Antimicrobial Resistance." Antibiotics 12, no. 3 (2023): 544. http://dx.doi.org/10.3390/antibiotics12030544.

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Streptococcus agalactiae or group B streptococcus (GBS) is a leading cause of neonatal sepsis and increasingly found as an invasive pathogen in older patient populations. Beta-lactam antibiotics remain the most effective therapeutic with resistance rarely reported, while the majority of GBS isolates carry the tetracycline resistance gene tet(M) in fixed genomic positions amongst five predominant clonal clades. In the UK, GBS resistance to clindamycin and erythromycin has increased from 3% in 1991 to 11.9% (clindamycin) and 20.2% (erythromycin), as reported in this study. Here, a systematic inv

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Baranowski, Eric, Emilie Dordet-Frisoni, Eveline Sagné, et al. "The Integrative Conjugative Element (ICE) ofMycoplasma agalactiae: Key Elements Involved in Horizontal Dissemination and Influence of Coresident ICEs." mBio 9, no. 4 (2018). http://dx.doi.org/10.1128/mbio.00873-18.

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ABSTRACTThe discovery of integrative conjugative elements (ICEs) in wall-less mycoplasmas and the demonstration of their role in massive gene flows within and across species have shed new light on the evolution of these minimal bacteria. Of these, the ICE of the ruminant pathogenMycoplasma agalactiae(ICEA) represents a prototype and belongs to a new clade of the Mutator-like superfamily that has no preferential insertion site and often occurs as multiple chromosomal copies. Here, functional genomics and mating experiments were combined to address ICEA functions and define the minimal ICEA chas

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Liu, Meng, Jialin Liu, Guitian Liu, et al. "ICEO, a biological ontology for representing and analyzing bacterial integrative and conjugative elements." Scientific Data 9, no. 1 (2022). http://dx.doi.org/10.1038/s41597-021-01112-5.

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AbstractBacterial integrative and conjugative elements (ICEs) are highly modular mobile genetic elements critical to the horizontal transfer of antibiotic resistance and virulence factor genes. To better understand and analyze the ongoing increase of ICEs, we developed an Integrative and Conjugative Element Ontology (ICEO) to represent the gene components, functional modules, and other information of experimentally verified ICEs. ICEO is aligned with the upper-level Basic Formal Ontology and reuses existing reliable ontologies. There are 31,081 terms, including 26,814 classes from 14 ontologie

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