Academic literature on the topic 'Interactant'

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Journal articles on the topic "Interactant"

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Hasan, Ruqaiya. "Tenor." Language, Context and Text 2, no. 2 (2020): 213–333. http://dx.doi.org/10.1075/langct.00029.has.

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Abstract This paper presents work-in-progress on the contextual variable tenor, here reconceptualised as ‘interactant relations’ in order to explore a radically different view of the relations of the interactants to the text in context. The functions of speaker/addressee are the starting point of an exploration of interactant relations because they represent the only features with the capacity to manage a text’s processes. This view implies that speaker/addressee have the capacity to internalise communal conventions, which places them at the centre of the language process. Implications of the results of the exploration for the classification of register are proposed. The paper’s methodology extends previous work on contextual networks (see especially Hasan 2001a, 2009b, 2014, 2016).i
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Remmert, Kirsten, Thomas E. Olszewski, M. Blair Bowers, Mariana Dimitrova, Ann Ginsburg, and John A. Hammer. "CARMIL Is aBona FideCapping Protein Interactant." Journal of Biological Chemistry 279, no. 4 (2003): 3068–77. http://dx.doi.org/10.1074/jbc.m308829200.

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Saltz, Julia B. "Genetic composition of social groups influences male aggressive behaviour and fitness in natural genotypes of Drosophila melanogaster." Proceedings of the Royal Society B: Biological Sciences 280, no. 1771 (2013): 20131926. http://dx.doi.org/10.1098/rspb.2013.1926.

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Indirect genetic effects (IGEs) describe how an individual's behaviour—which is influenced by his or her genotype—can affect the behaviours of interacting individuals. IGE research has focused on dyads. However, insights from social networks research, and other studies of group behaviour, suggest that dyadic interactions are affected by the behaviour of other individuals in the group. To extend IGE inferences to groups of three or more, IGEs must be considered from a group perspective. Here, I introduce the ‘focal interaction’ approach to study IGEs in groups. I illustrate the utility of this approach by studying aggression among natural genotypes of Drosophila melanogaster. I chose two natural genotypes as ‘focal interactants’: the behavioural interaction between them was the ‘focal interaction’. One male from each focal interactant genotype was present in every group, and I varied the genotype of the third male—the ‘treatment male’. Genetic variation in the treatment male's aggressive behaviour influenced the focal interaction, demonstrating that IGEs in groups are not a straightforward extension of IGEs measured in dyads. Further, the focal interaction influenced male mating success, illustrating the role of IGEs in behavioural evolution. These results represent the first manipulative evidence for IGEs at the group level.
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Obana, Yasuko. "Speech level shifts in Japanese." Pragmatics. Quarterly Publication of the International Pragmatics Association (IPrA) 26, no. 2 (2016): 247–90. http://dx.doi.org/10.1075/prag.26.2.04oba.

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The present paper analyses speech level shifts in Japanese from a different perspective. By applying Symbolic InteractionistRole Theory, speech level shifts are categorised as the linguistic realisation of aninteractional role, or ‘dissociative role’ Icall in this paper. Dissociative roles are improvised identities, which occur when the speaker perceives a psychological change in relation to the other participant in the on-going interaction. Plus-level shifts (shifts from plain to polite forms, masu/desu) are triggered when the speaker experiences cautious, attentive, thoughtful and/or grateful feelings at a certaintime of interaction, which conforms to the original nature of honorifics. This prompts a dissociative role which creates a certain psychological distance between this role and the other interactant. On the other hand, minus-level shifts (shifts from masu/desu forms to plain forms) are the implementation of the speaker’s another dissociative role, which is assimilated with the other interactant, giving rise to empathy or drawing the other into the speaker’s world. Whether plus or minus level shifts occur, the interactants’social roles, i.e.,their original roles when the situation is defined, continue to exist throughout the discourse. The interactants are fully aware of their social roles such as teacher and student, friends, family members, and senior and junior in company(= Institutional Roles in this paper). However, when an Improvised Role is created, it is forwarded to the on-going interaction and linguistically implemented as a speech level shift.This paper also clarifies that both speech level shifts and the so-called ‘conventional’honorifics are situationally determined, and that they are not separate entities but the two ends of continuum by examining the features they share from the viewpoint of ‘roles’.
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Halualani, Rona Tamiko. "Interactant-Based Definitions of Intercultural Interaction at a Multicultural University." Howard Journal of Communications 21, no. 3 (2010): 247–72. http://dx.doi.org/10.1080/10646175.2010.496666.

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Dzieweczynski, Teresa L., Nicole E. Greaney, Kelley B. Portrais, and Megan A. Stevens. "I remember you: female Siamese fighting fish recognise prior social partners." Behaviour 154, no. 1 (2017): 19–35. http://dx.doi.org/10.1163/1568539x-00003409.

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Recognising social partners allows individuals to establish social networks with one another, informs mating decisions, and decreases energy expenditure. Studies rarely examine if females have this ability outside of mate choice. Additionally, it is unknown if familiarity differs when females encounter females versus males. Female Siamese fighting fish were placed into one of six treatment groups that differed based on the sex of the interactant (female or male) and experience (familiar, unfamiliar or no previous exposure). In both female–female and female–male interactions, less behaviour was performed towards familiar individuals. However, the degree to which familiarity had an effect differed depending on the sex of the interactant and the behaviour measured. Familiarity may serve an important function if it increases an individual’s ability to remember the outcome of prior encounters and use this information in later encounters with the same individual. To fully understand social dynamics, both sexes must be examined.
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Tavares, Sinivaldo Silva. "Transmissão da fé e cultura midiática. Disquisições de um teólogo católico." Revista Eclesiástica Brasileira 80, no. 315 (2020): 86. http://dx.doi.org/10.29386/reb.v80i315.2023.

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Após descrever o cenário atual como “cumplicidade” entre mercado, tecnociência e mídia, o objetivo que se propõe aqui é discernir desafios e chances postos pela complexa incumbência de transmitir a fé no horizonte da cultura midiática vigente, caracterizada, em modo especial, pela reconexão: um interagente constrói um conteúdo simbólico que, por sua vez, é recebido e reconhecido por outro interagente em conexão e, por conseguinte, reconstruído, mediante novas conexões, por outros interagentes ainda. Por fim, na ótica de um teólogo de tradição católica, são elencados três dos principais desafios postos por esse diálogo intercultural: oportunidade de se conceber missão como evangelização; incessante processo de deconstrução e reconstrução da catolicidade e possibilidade de ressignificar a própria catolicidade.Abstract:After describing the current scenario as “complicity” between market, technoscience and media, the objective proposed here is to discern challenges and chances posed by the complex task of transmitting faith in the horizon of the current media culture, characterized, in a special way, by reconnection : an interactant builds a symbolic content that, in turn, is received and recognized by another interacting person in connection and, therefore, reconstructed, through new connections, by other interactants. Finally, from the perspective of a theologian with a Catholic tradition, three of the main challenges posed by this intercultural dialogue are listed: opportunity to conceive mission as evangelization; incessant process of deconstruction and reconstruction of catholicity and the possibility of reframing catholicity itself.Keywords: Media culture; Reconnection; Evangeliztion; Catholicity.
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Gipper, Sonja. "Repeating responses as a conversational affordance for linguistic transmission." Studies in Language 44, no. 2 (2020): 281–326. http://dx.doi.org/10.1075/sl.19041.gip.

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Abstract Given that face-to-face interaction is an important locus for linguistic transmission (Enfield 2008: 297), it is argued in this paper that conversational structure must provide affordances (Gibson 1979) for transmitting linguistic items. The paper focuses on repeats where an interactant (partially) repeats their interlocutor’s preceding utterance. Repeats are argued to provide affordances for the transmission of innovative and conservative linguistic items by forcing interactants to repeat linguistic material uttered by another person, facilitating production by exploiting priming effects. Moreover, repeats leave room for modification and thereby for actively resisting transmission. In this way, repeats unite the competing forces (Tantucci et al. 2018) of automaticity and creativity. To support this claim, this paper investigates the use of Spanish insertions and alternative variants in utterance-repeat pairs in Yurakaré (isolate, Bolivia) conversations. The findings are compatible with a holistic view of language where all linguistic levels are interconnected (Beckner et al. 2009).
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Turner, Elizebeth C., Eamon P. Mulvaney, Helen M. Reid, and B. Therese Kinsella. "Interaction of the human prostacyclin receptor with the PDZ adapter protein PDZK1: role in endothelial cell migration and angiogenesis." Molecular Biology of the Cell 22, no. 15 (2011): 2664–79. http://dx.doi.org/10.1091/mbc.e11-04-0374.

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Prostacyclin is increasingly implicated in re-endothelialization and angiogenesis but through largely unknown mechanisms. Herein the high-density lipoprotein (HDL) scavenger receptor class B, type 1 (SR-B1) adapter protein PDZ domain-containing protein 1 (PDZK1) was identified as an interactant of the human prostacyclin receptor (hIP) involving a Class I PDZ ligand at its carboxyl terminus and PDZ domains 1, 3, and 4 of PDZK1. Although the interaction is constitutive, it may be dynamically regulated following cicaprost activation of the hIP through a mechanism involving cAMP-dependent protein kinase (PK)A-phosphorylation of PDZK1 at Ser-505. Although PDZK1 did not increase overall levels of the hIP, it increased its functional expression at the cell surface, enhancing ligand binding and cicaprost-induced cAMP generation. Consistent with its role in re-endothelialization and angiogenesis, cicaprost activation of the hIP increased endothelial cell migration and tube formation/in vitro angiogenesis, effects completely abrogated by the specific IP antagonist RO1138452. Furthermore, similar to HDL/SR-B1, small interfering RNA (siRNA)-targeted disruption of PDZK1 abolished cicaprost-mediated endothelial responses but did not affect VEGF responses. Considering the essential role played by prostacyclin throughout the cardiovascular system, identification of PDZK1 as a functional interactant of the hIP sheds significant mechanistic insights into the protective roles of these key players, and potentially HDL/SR-B1, within the vascular endothelium.
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Ait Ammar, Amina, Virginie Gautier, Carine Van Lint, Christian Schwartz, and Olivier Rohr. "5 Inhibition of HIV-1 gene transcription by KAP1: a new CTIP2 interactant." Journal of Virus Eradication 3 (July 2017): 7. http://dx.doi.org/10.1016/s2055-6640(20)30710-x.

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Dissertations / Theses on the topic "Interactant"

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MADANIA, AMMAR. "Etude fonctionnelle des proteines apparentees a l'actine, arp2 et arp3 ; recherche de proteines interactant chez la levure saccharomyces cerevisiae." Université Louis Pasteur (Strasbourg) (1971-2008), 1998. http://www.theses.fr/1998STR13083.

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Ce travail porte sur la caracterisation de deux proteines apparentees a l'actine arp2 et arp3 (actin related protein), chez la levure s. Cerevisiae. Par l'analyse cytologique de mutants thermosensibles arp2 et arp3, nous avons montre que ces deux proteines sont essentielles pour l'organisation du cytosquelette d'actine et pour l'endocytose. Un crible double-hybride nous a permis d'identifier plusieurs partenaires d'arp2p , dont arp2p elle-meme, l'actine et le facteur d'elongation ef1, recemment identifie comme une proteine fixant l'actine. De plus, arp2p et arp3p interagissent genetiquement et dans le systeme double-hybride, confirmant l'existance de ces deux arps dans un complexe multiproteique. Nous avons montre que la surexpression de las17p est capable de supprimer d'une facon allele-specifique les defauts de croissance, d'organisation du cytosquelette d'actine et d'endocytose aussi bien de mutants arp2 que arp3 ts. De plus, arp2p, arp3p et las17p colocalisent avec les taches corticales d'actine. Las17p est homologue de la proteine wasp (wiskott-aldrich syndrome protein) impliquee dans une maladie genetique humaine d'immunodeficience. La deletion du gene las17 dans la levure entraine soit la letalite, soit la thermosensibilite, selon le contexte genetique. Les cellules las17 viables accumulent des agregats aberrants d'actine, semblables aux agregats observes dans les mutants arp2 et arp3 ts. Grace a un crible double-hybride, nous avons identifie des partenaires de las17p, dont l'actine, la verproline, et plusieurs proteines a domaine sh3, suggerant que las17p est impliquee dans des voies de signalisation et de regulation du cytosquelette d'actine. L'interaction de las17p avec lsb6p (l'homologue de cip4p humaine) suggere un lien entre las17p et cdc42p. Nos resultats convergent vers l'implication d'arp2p, arp3p et las17p dans l'organisation du cytosquelette cortical d'actine, probablement par un mecanisme de nucleation et de stabilisation des filaments d'actine.
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Ma, Huailiang, and 马怀良. "Identification of human annexin A6 as a novel cellular interactant of influenza A virus M2 protein and regulator of virus budding andrelease." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48521747.

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Influenza viruses exploit sophisticated host cell machinery to replicate, causing both seasonal epidemics and unpredictable pandemics. Studying the host cellular factors interacting with conserved domains of viral proteins will help us to identify key host proteins for the virus infection. This will not only strengthen our understanding of the precise mechanisms of the virus life cycle, but also pave new avenues for anti-viral development. The cytoplasmic tail of M2 ion channel (M2/CT) is one of these highly conserved domains. It is fully accessible to the host cell machinery after fusion of the virus envelope with the endosomal membrane and during the trafficking, assembly, and budding processes. I hypothesized that recruitment of host cellular factors by M2/CT may regulate the M2-dependent stages of the virus life cycle. Through a large scale yeast two-hybrid (Y2H) screen with the M2/CT used as bait, the human annexin A6 was identified as a novel host cell interactant and this interaction was further confirmed by both GST pull-down assay on purified proteins and co-immunoprecipitation assay on virus infected cells. A functional characterization of this novel interaction demonstrated that depletion of annexin A6 could enhance the virus production, while its overexpression could reduce the virus propagation, which indicates that annexin A6 is a negative regulator of the virus infection. However, I found that the virus infection could not induce any changes of annexin A6 expression. Therefore, the annexin A6-mediated regulation may depend on the subcellular localization where the interaction with M2/CT occurs. To decipher which step of the virus replication is regulated, we dissected the virus life cycle and found that modulation of annexin A6 expression had no effect on the early stages of the virus life cycle or on viral RNA replication but impaired the release of progeny virus, as suggested by delayed or defective budding events observed at the plasma membrane of virus-infected and annexin A6-overexpressing cells during a transmission electron microscopy study. To further decipher the underlying molecular mechanisms, the contribution of annexin A6-mediated plasma membrane lipid rafts reorganization through cholesterol homeostasis modulation and cortical actin cytoskeleton remodeling was also investigated. In conclusion, here I have identified the human annexin A6 as a novel host cell interactant of M2/CT that negatively modulate the influenza virus infection by impairing the virus budding and release. This work further supports the idea that M2 is a multifunctional protein and is also consistent with the discovery by Rossman et al. that M2/CT mediates the virus budding process (Rossman et al., 2010). This study further emphasizes the importance of host cell interactants of M2/CT in this process. Regarding the biology of annexins, this study also adds a new member of this protein family in the list of regulators of influenza virus infection.<br>published_or_final_version<br>Public Health<br>Doctoral<br>Doctor of Philosophy
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Malicorne, Sébastien. "Recherche d’interactants du domaine immunosuppresseur des protéines d’enveloppe rétrovirales." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS579.

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La plupart des virus ont développé des mécanismes de résistance ou de suppression du système immunitaire pour parvenir à infecter durablement leur hôte. Ces mécanismes demeurent encore imparfaitement connus. Un domaine immunosuppresseur (IS) a été identifié au niveau de la région transmembranaire des protéines d’enveloppe des rétrovirus endogènes ou infectieux. Ce domaine hautement conservé a été décrit par exemple comme inhibant l’activation lymphocytaire. Dans le laboratoire, il a été caractérisé en particulier via des expériences de rejet de cellules tumorales in vivo, ce qui a permis de définir des mutations inactivatrices. Afin de mieux comprendre les mécanismes de résistance des rétrovirus au système immunitaire, mes travaux de thèse ont porté sur l’identification de la ou des protéines capables d’interagir avec le domaine IS. Plusieurs approches cellulaires et moléculaires ont été développées, basées pour la plupart sur l’utilisation de sondes fluorescentes obtenues par synthèse chimique, constituées des domaines IS provenant de différents rétrovirus. Dans un premier temps, les cellules du système immunitaire qui lient les protéines virales ont été identifiées : les lymphocytes B et les cellules myéloïdes (monocytes, cellules dendritiques et macrophages). Dans un second temps, des expériences de co-immunoprécipitation et de chromatographie d’affinité couplées à la spectrométrie de masse ont été réalisées dans le but d’identifier sur ces cellules les protéines membranaires responsables de ces liaisons. Plusieurs agents de couplages chimiques ont été utilisés afin de maintenir les liaisons domaine IS - protéine de faibles affinités. En raison de résultats non-reproductibles obtenus au cours de ces expériences, des tests de liaison du domaine IS sur des cellules transfectées avec des banques d’ADNc, ou lors d’expériences de double hybride ont été réalisées. Ces deux approches ont permis d’identifier des protéines membranaires potentiellement impliquées dans la liaison du domaine IS : les protéines X1 et X2. Les co-transfections de vecteurs d’expression du domaine IS et de X2 ont mis en évidence des interactions protéiques au cours d’expériences de co-immunoprécipitation et de microscopie confocale, en particulier avec le domaine IS du rétrovirus HIV-1. Concernant X1, sa transfection induit la liaison cellulaire des domaines IS de HERV-W et MLV. En revanche, aucune interaction directe entre X1 et le domaine IS n’a pu être démontrée, notamment dans des expériences de co-immunoprécipitation et de microscopie confocale.La découverte des protéines membranaires qui interagissent avec le domaine IS demeure un enjeu critique pour la compréhension des voies de signalisation et de transcription qui permettent aux rétrovirus d’exercer leur effet sur le système immunitaire, l’objectif de ces travaux étant d’identifier à terme des nouvelles cibles thérapeutiques.En conclusion, même si des travaux complémentaires demeurent nécessaires, les protéines X1 et X2 pourraient contribuer à l’immunosuppression rétrovirale<br>Most viruses have developed mechanisms of resistance or suppression of the immune system to achieve lasting infection of their host. These mechanisms are still imperfectly known. An immunosuppressive (IS) domain has been identified in the transmembrane region of envelope proteins of endogenous or infectious retroviruses. This highly conserved domain has been described, for example, as inhibiting lymphocyte activation. In the laboratory, it has been characterized by tumor cell rejection experiments in vivo, which has made it possible to define inactivating mutations. In order to better understand the mechanisms of resistance of retroviruses to the immune system, my thesis focused on the identification of the protein(s) interacting with the IS domain. Several cellular and molecular approaches have been developed, based for the most part on the use of fluorescent probes obtained by chemical synthesis, consisting of IS domains from different retroviruses. At first, immune system cells that bind viral proteins have been identified: B cells and myeloid cells (monocytes, dendritic cells and macrophages). In a second step, co-immunoprecipitation and affinity chromatography coupled to mass spectrometry were performed to identify on these cells the membrane proteins responsible for these bonds. Several chemical coupling agents have been used to prevent detachment of low affinity binding between proteins and the IS domain. Due to non-reproducible results obtained during these experiments, IS domain binding assays on cells transfected with cDNA libraries, or in double hybrid experiments were performed. These two approaches made it possible to identify membrane proteins potentially involved in the binding of the IS domain: the X1 and X2 proteins. Co-transfections of IS domain and X2 expression vectors demonstrated protein interactions in co-immunoprecipitation and confocal microscopy experiments, particularly with the IS domain of the HIV-1 retrovirus. Concerning X1, its transfection induces binding of the IS domains of HERV-W and MLV on cells membrane. On the other hand, no direct interaction between X1 and the IS domain could be demonstrated, especially in co-immunoprecipitation and confocal microscopy experiments.The discovery of membrane proteins that interact with the IS domain remains a critical issue for understanding the signaling and transcription pathways that allow retroviruses to exert their effect on the immune system, the aim of this work being to identify new therapeutic targets.In conclusion, although further work is still needed, the X1 and X2 proteins may contribute to retroviral immunosuppression
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Hart, Alison Claire. "Interacting thermals." Thesis, University of Cambridge, 2008. https://www.repository.cam.ac.uk/handle/1810/252113.

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This thesis addresses the question of how thermals evolve and interact when released in proximity to one another. The research reported focuses on the simultaneous release of an isolated group of thermals in a larger domain, rather than an array of thermals spanning a whole horizontal plane. With multiple thermals, the fluid available between the thermals is limited and this might be expected to lead to competition between the thermals for the surrounding fluid. The evolution of the thermals and the interaction between them will depend on their initial separation. If released close enough to each other there will be mixing between them and, in some cases, merging, whereas if the separation is large compared with the depth of the domain they may be expected to develop as if in isolation. Initially, the results for a thermal released in isolation are studied and the sensitivity to changes in initial buoyancy and the arrangement of fluid are examined. Having determined the experimental constants for an isolated thermal with certain initial conditions, the change in behaviour from the isolated self-similar case due to the presence of a second thermal is considered. A surprising lack of interaction between the thermals before the point at which the thermals first come into contact is discovered and a possible model for two thermals released in proximity to each other is suggested. In order to better understand the merging of the thermals the flow is considered from different angles of view. The distribution of mass within each two-dimensional projection is examined and compared to that of an idealised spherical thermal with uniform density as well as other possible models for a thermal.
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Mikitova, Veronika. "CDKA interacting proteins." Thesis, University of East Anglia, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502199.

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Progression through the cell cycle relies on activity of cyclin-dependent kinases (CDKs) and their regulatory subunits, cyclins. CDKA and CDKB are the main components of the G2/M transition point in Arabidopsis thaliana. I aim to gain a better understanding of how mitotic kinases, CDKA and CDKB. mediate control of translation initiation and other biological processes throughout the cell cycle. To address this question, I used different approaches to identify interacting partners of CDKA and CDKB (and other cell cycle regulators). CDKA directly interacts with the complex that binds the 5' mRNA cap. In proliferating cells, CDKA associates with translation initiation factor eIF4A as demonstrated by reciprocal immonoprecipitation (Hutchins et al., 2004). To understand this interaction in greater depth, I used a pair-wise yeast two hybrid to test interactions between cell cycle regulators and translation initiation factors. I confirmed the association of translation initiation factors that is in agreement with the mechanism of cap complex assembly in animals and yeast and identified eIF4A and eIF4E as a putative substrates of both CDKA and CDKB2;1.
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Graham, B. T. "Interacting stochastic systems." Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599589.

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The Ising model was suggested by Lenz in 1920. It is a probabilistic model for ferromagnetism. Magnetization can then be explored as correlation between spin random variables on a graph. Bond percolation was introduced by Simon Broadbent and John Hammersley in 1957. It is a model for long range order. Edges of a lattice graph are declared open, independently, with some probability <i>p</i>, and clusters of open edges are studied. Both these models can be understood as aspects of the random-cluster model. In this thesis we study various aspects of mathematical statistical mechanics. In Chapter 2 we create a diluted version of the random-cluster model. This allows the coupling of the Ising model to the random-cluster model to be extended to include the Blume-Capel model. Crucially, it retains some of the key properties of its parent model. This enables much of the random-cluster technology to be carried forward. The key issue for bond percolation concerns the fraction of open edges required in order to have long range connectivity. Harry Kesten proved that this fraction is precisely one half for the square planar lattice. Recent development in the theory of influence and sharp thresholds allowed Béla Bollobás and Oliver Riordan to simplify parts of his proof. In Chapter 3 we extend an influence result to apply to monotonic measures. This allows sharp thresholds to be shown for certain families of stochastically increasing monotonic distributions, including random-cluster measures. In Chapter 4 we study time to convergence for a mean-field zero-range process. The problem was motivated by the canonical ensemble model of energy levels used in the derivation of Maxwell-Boltzmann distributions. By considering the entropy of the system, we show that the empirical distribution rapidly converges – in the sense of Kullback-Leibler divergence – to a geometric distribution. The proof utilizes arguments of spectral gap and log Sobolev type.
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Curk, Tine. "Modelling multivalent interactons." Thesis, University of Cambridge, 2016. https://www.repository.cam.ac.uk/handle/1810/266916.

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A Multivalent entity, which could represent a protein, nanoparticle, polymer, virus or a lipid bilayer, has the ability to form multiple bonds to a substrate. Hence, a multivalent interaction can be strong, even if the individual bonds are weak. However, much more interestingly, multivalency enables the design of highly specific interactions using non-specific individual bonds. We attempt to rationalise multivalent effects using simple physical models complemented with numerical simulations. Based on physiochemical characteristics of multivalent binders, we aim to predict the overall strength of interaction and its sensitivity to variation in parameters. We start with a simple model of homo-multivalency, where all bonds are equivalent. Such systems can exhibit a super-selective response, which denotes the high sensitivity of the strength of multivalent binding to the number of accessible binding sites on the target surface. We present a theoretical analysis of systems of multivalent particles and show that a certain degree of disorder is necessary for super-selective behaviour. Moreover, we formulate a set of simple design rules for multivalent interactions that yield optimal selectivity. In the second stage, we expand the model to hetero-multivalency, accounting for multiple distinct types of binding partners. We consider targeting of cells based on a density profile of different membrane receptors types and demonstrate, that speci city towards a desired receptor density profile can be obtained. Hence, cells can be reliably targeted in the absence of specific markers. Crucially, we show that for optimal selectivity, individual bonds must be weak. Finally, we add information about specific geometry and positions of binding sites on the multivalent entity. We focus on molecular imprinting; the process whereby a polymer matrix is cross-linked in the presence of template molecules. The cross-linking process endows the polymer matrix with a chemical ‘memory’, such that the target molecules can subsequently be recognised by the matrix. We show how the binding multivalency and the polymer material properties affect the efficiency and selectivity of molecular imprinting.
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Whitehead, Thomas Michael. "Interacting Fermi gases." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274548.

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Interacting Fermi gases are one of the chief paradigms of condensed matter physics. They have been studied since the beginning of the development of quantum mechanics, but continue to produce surprises today. Recent experimental developments in the field of ultracold atomic gases, as well as conventional solid state materials, have produced new and exotic forms of Fermi gases, the theoretical understanding of which is still in its infancy. This Thesis aims to provide updated tools and additional insights into some of these systems, through the application of both numerical and analytical techniques. The first Part of this Thesis is concerned with the development of improved numerical tools for the study of interacting Fermi gases. These tools take the form of accurate model potentials for the dipolar and contact interactions, as found in various ultracold atomic gas experiments, and a new form of Jastrow correlation factor that interpolates between the radial symmetry of the inter-electron Coulomb potential at short inter-particle distances, and the symmetry of the numerical simulation cell at large separation. These methods are designed primarily for use in quantum Monte Carlo numerical calculations, and provide high accuracy along with considerable acceleration of simulations. The second Part shifts focus to an analytical analysis of spin-imbalanced Fermi gases with an attractive contact interaction. The spin-imbalanced Fermi gas is shown to be unstable to the formation of multi-particle instabilities, generalisations of a Cooper pair containing more than two fermions, and then a theory of superconductivity is built from these instabilities. This multi-particle superconductivity is shown to be energetically favourable over conventional superconducting phases in spin-imbalanced Fermi gases, and its unusual experimental consequences are discussed.
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Martins, Soraia Alexandra Araújo. "CD81 interacting proteins." Master's thesis, Universidade de Aveiro, 2016. http://hdl.handle.net/10773/16139.

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Mestrado em Biomedicina Molecular<br>Fertilization is a multistep and complex process culminating in the merge of gamete membranes, cytoplasmic unity and fusion of genome. CD81 is a tetraspanin protein that participates in sperm-oocyte interaction, being present at the oocyte surface. CD81 has also been implicated in other biological processes, however its specific function and molecular mechanisms of action remain to be elucidated. The interaction between CD81 and its binding partner proteins may underlie the CD81 involvement in a variety of cellular processes and modulate CD81/interactors specific functions. Interestingly, in a Yeast two Hybrid system previously performed in our lab, CD81 has emerged as a putative interactor of the Amyloid Precursor Protein (APP). In the work here described, bioinformatics analyses of CD81 interacting proteins were performed and the retrieved information used to construct a protein-protein interaction network, as well as to perform Gene Ontology enrichment analyses. CD81 expression was further evaluated in CHO, GC-1 and SH-SY5Y cell lines, and in human sperm cells. Additionally, its subcellular localization was analyzed in sperm cells and in the neuronal-like SH-SY5Y cell line. Subsequently, coimmunoprecipitation assays were performed in CHO and SH-SY5Y cells to attempt to prove the physical interaction between CD81 and APP. A functional interaction between these two proteins was accessed thought the analyses of the effects of CD81 overexpression on APP levels. A co-localization analysis of CD81 and some interactors proteins retrieved from the bioinformatics analyses, such as APP, AKT1 and cytoskeleton-related proteins, was also performed in sperm cells and in SH-SY5Y cells. The effects of CD81 in cytoskeleton remodeling was evaluated in SH-SY5Y cells through monitoring the effects of CD81 overexpression in actin and tubulin levels, and analyzing the colocalization between overexpressed CD81 and F-actin. Our results showed that CD81 is expressed in all cell lines tested, and also provided the first evidence of the presence of CD81 in human sperm cells. CD81 immunoreactivity was predominantly detected in the sperm head, including the acrosome membrane, and in the midpiece, where it co-localized with APP, as well as in the post-acrosomal region. Furthermore, CD81 co-localizes with APP in the plasma membrane and in cellular projections in SH-SY5Y cells, where CD81 overexpression has an influence on APP levels, also visible in CHO cells. The analysis of CD81 interacting proteins such as AKT1 and cytoskeletonrelated proteins showed that CD81 is involved in a variety of pathways that may underlie cytoskeleton remodeling events, related to processes such as sperm motility, cell migration and neuritogenesis. These results deepen our understanding on the functions of CD81 and some of its interactors in sperm and neuronal cells.<br>A fecundação é um processo complexo e faseado que culmina na fusão celular das membranas dos gametas, do citoplasma e do genoma. A CD81 é uma proteína tetraspanina que participa na interacção espermatozóide-oócito, estando presente na superfície do oócito. A CD81 também tem sido associada a outros processos biológicos, contudo a sua função específica e os seus mecanismos de acção não estão elucidados. A ligação entre a CD81 e as suas proteínas interactoras fundamenta o envolvimento da CD81 numa variedade de processos celulares e funções específicas. O desenvolvimento de um sistema de Dois Híbrido em Leveduras, anteriormente realizado no nosso laboratório, mostrou que a CD81 potencialmente interage com a Proteína Percursora de Amilóide (PPA). No presente trabalho, foi realizada a análise bioinformática das proteínas interactoras da CD81. A informação obtida permitiu a construção de uma rede de interações proteína-proteína, bem como a análise de enrequecimento de Ontologia de Genes. Adicionalmente, a expressão da CD81 foi avaliada nas linhas celulares CHO, GC-1 e SH-SY5Y e em espermatozóides humanos. A sua localização subcelular foi também analisada em espermatozóides humanos e na linha de neuroblastoma SH-SY5Y. Foram ainda realizados ensaios de coimunoprecipitacão nas linhas celulares CHO e SH-SY5Y, com a tentativa de provar a intercação física entre a CD81 e a PPA. A interação funcional entre estas duas proteínas foi estudada através da análise do efeito da sobreexpressão da CD81 nos níveis de PPA. Foram também realizados estudos de colocalização entre a CD81 e algumas proteínas interactoras, nos espermatozóides humanos e na linha celular SH-SY5Y. Os interatores analisados, PPA, AKT1 e proteínas relacionadas com o citoesqueleto, foram obtidos da análise bioinformática previamente realizada. O efeito da CD81 na remodelação do citoesqueleto foi avaliado através da monitorização dos efeitos da sobre-expressão da CD81 nos níveis de actina e tubulina, bem como através da análise da colocalização entre a CD81 sobre-expressa e a F-actina. Os nossos resultados mostram que a CD81 está expressa em todas as linhas celulares testadas, providenciando a primeira evidência da presença da CD81 em espermatozóides humanos. A marcação da CD81 foi predominantemente detectada na cabeça do espermatozóde e na peça intermédia, onde colocaliza com a PPA, bem como na região pós-acrossómica. Em adição, a CD81 colocaliza com a PPA na membrana plasmática e nas projecções celulares das células SH-SY5Y, onde a sobre-expressão da CD81 influencia os níveis de PPA, efeito também observado nas células CHO. A análise de proteínas interactoras da CD81, como a AKT1 e proteínas relacionadas com o citoesqueleto, evidenciou que a CD81 está envolvida na remodelação do citoesqueleto, nomeadamente na motilidade dos espermatozóides, na migração celular e na neuritogénese. Estes resultados permitiram aprofundar o conhecimento das funções da CD81 e de alguns dos seus interactores, em espermatozóides e em células neuronais.
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Boring, Sebastian. "Interacting "Through the Display"." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-118188.

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Books on the topic "Interactant"

1

Shore, Steven N. Interacting binaries. Springer-Verlag, 1994.

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1940-, Eggleton P. P., Pringle J. E. 1949-, and North Atlantic Treaty Organization. Scientific Affairs Division., eds. Interacting binaries. D. Reidel Pub. Co., 1985.

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Eggleton, P. P., and J. E. Pringle, eds. Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6.

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Tyler, V. Lynn. Intercultural interacting. David M. Kennedy Center for International Studies, Brigham Young University, 1987.

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Shore, S. N., M. Livio, and E. P. J. van den Heuvel. Interacting Binaries. Edited by H. Nussbaumer and A. Orr. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/3-540-31626-4.

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Tarabilda, Edward F. Interacting with society. Passage Press, 1990.

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Liggett, Thomas M. Interacting particle systems. Springer, 2005.

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Interacting particle systems. Springer-Verlag, 1985.

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Interacting particle systems. Springer-Verlag, 1985.

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Robertson, Margaret E., ed. Communicating, Networking: Interacting. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-45471-9.

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Book chapters on the topic "Interactant"

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Smedslund, Jan. "Interacting." In Psycho-Logic. Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73121-1_8.

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Delina, Laurence L. "Interacting." In Climate Actions. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-91884-6_6.

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Kenyon, Scott J. "Symbiotic Stars." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_8.

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Ilovaisky, Sergio A. "Optical Characteristics of X-Ray Binaries." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_9.

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Griffin, R. F. "The Distributions of Periods and Amplitudes of Late-Type Spectroscopic Binaries." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_1.

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White, N. E. "Massive X-Ray Binaries." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_10.

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Wade, Richard A. "Cataclysmic Variables as Interacting Binary Stars." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_11.

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Horne, Keith. "Maximum Entropy Reconstruction Of Accretion Disk Images From Eclipse Data." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_12.

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Nather, R. E. "The Late Stages Of Interactive Stellar Evolution." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_13.

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Warner, Brian. "Stars That Go Hump in the Night: The SU UMa Stars." In Interacting Binaries. Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5337-6_14.

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Conference papers on the topic "Interactant"

1

DeLappe, Joseph. "Masturbatory interactant." In ACM SIGGRAPH 96 Visual Proceedings: The art and interdisciplinary programs of SIGGRAPH '96. ACM Press, 1996. http://dx.doi.org/10.1145/253607.253620.

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Baillie, Lynne, David Beattie, and Lee Morton. "Feel what you hear." In Interacting with Sound Workshop. ACM Press, 2011. http://dx.doi.org/10.1145/2019335.2019336.

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Albrecht, Robert, Tapio Lokki, and Lauri Savioja. "A mobile augmented reality audio system with binaural microphones." In Interacting with Sound Workshop. ACM Press, 2011. http://dx.doi.org/10.1145/2019335.2019337.

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McGookin, David, and Stephen Brewster. "PULSE." In Interacting with Sound Workshop. ACM Press, 2011. http://dx.doi.org/10.1145/2019335.2019338.

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Sandholm, Thomas, and Hang Maxime Ung. "Turn-by-turn directions go social." In Interacting with Sound Workshop. ACM Press, 2011. http://dx.doi.org/10.1145/2019335.2019339.

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Kizelshteyn, Boris, Andrew Lippman, and Victor Hung. "X-Ray Audio." In Interacting with Sound Workshop. ACM Press, 2011. http://dx.doi.org/10.1145/2019335.2019340.

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Ankolekar, Anupriya, and Thomas Sandholm. "Foxtrot." In Interacting with Sound Workshop. ACM Press, 2011. http://dx.doi.org/10.1145/2019335.2019341.

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Ederoclite, A. "The early spectral evolution of nova Sgr 2004." In INTERACTING BINARIES: Accretion, Evolution, and Outcomes. AIP, 2005. http://dx.doi.org/10.1063/1.2130283.

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Skopal, A. "A multiple mass-ejection by the symbiotic prototype Z And during its 2000–03 outburst." In INTERACTING BINARIES: Accretion, Evolution, and Outcomes. AIP, 2005. http://dx.doi.org/10.1063/1.2130284.

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Exter, Katrina. "What happens when a hot star shines on a cool one?" In INTERACTING BINARIES: Accretion, Evolution, and Outcomes. AIP, 2005. http://dx.doi.org/10.1063/1.2130285.

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Reports on the topic "Interactant"

1

Blados, Walter R., and Charlie Maiorana. Interacting with DTIC. Defense Technical Information Center, 1990. http://dx.doi.org/10.21236/ada232485.

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Dagotto, Elbio. Computational Studies of Strongly Interacting Electrons. Defense Technical Information Center, 1997. http://dx.doi.org/10.21236/ada328576.

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Dykman, Mark, and Lora Billings. Controlling Interacting Systems in Noisy Environments. Defense Technical Information Center, 2010. http://dx.doi.org/10.21236/ada532768.

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Case, Steven T. Interacting Sites in Novel Polymeric Proteins. Defense Technical Information Center, 1995. http://dx.doi.org/10.21236/ada295830.

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Billings, Lora, and Mark Dykman. Controlling Interacting Systems in Noisy Environments. Defense Technical Information Center, 2010. http://dx.doi.org/10.21236/ada518960.

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Li, Andy C. Y., Alexandru Macridin, and Panagiotis Spentzouris. Variational Quantum Simulator of Interacting Bosons. Office of Scientific and Technical Information (OSTI), 2019. http://dx.doi.org/10.2172/1592132.

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Doerschuk, Peter C., Robert R. Tenney, and Alan S. Willsky. Modeling Electrocardiograms Using Interacting Markov Chains. Defense Technical Information Center, 1985. http://dx.doi.org/10.21236/ada162758.

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Kolb, E. W., M. S. Turner, and T. P. Walker. Effect of interacting particles on primordial nucleosynthesis. Office of Scientific and Technical Information (OSTI), 1986. http://dx.doi.org/10.2172/5525132.

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Hegarty, Mary. Individual Differences in Interacting With Hypermedia Manuals. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada426950.

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Varadhan, S. R. Interacting Particle Systems and Their Scaling Limits. Defense Technical Information Center, 1996. http://dx.doi.org/10.21236/ada308783.

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