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Dissertations / Theses on the topic 'Interactive CD'
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Chen, Chia-Chun. "Electronic music : an interactive CD-ROM for young people." FIU Digital Commons, 2003. http://digitalcommons.fiu.edu/etd/2129.
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The purpose of this thesis was to create an electronic music CD-ROM for young people. The intent of this project was to encourage young people to learn about the basics of sound production, audio effects, and the history of electronic music. The methods used for making this CD-ROM included having interactive demonstrations, animations, graphics. and a variety of colors and text styles. Internet links for further information were also provided.
This was the first educational CD-ROM of its kind. Although there were Internet sites and a few CD-ROMs covering issues of electronic music, most were not interactive and were not designed for young people. It was expected that this CD-ROM would make the learning process more interesting and would hopefully lead young people to more advanced materials on electronic music.
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Seitz, Jeffrey L. "An interactive CD-ROM to teach harmonic and intermodulation distortion." Virtual Press, 1997. http://liblink.bsu.edu/uhtbin/catkey/1048367.
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This study had two purposes: first, to collect information relating to harmonic and intermodulation distortion from several sources into one comprehensive report and second, to describe and develop an interactive CD-ROM as a new method of learning harmonic and intermodulation distortion. The CD-ROM allows students to interact and control the percentage amount of harmonic and intermodulation distortion in order to "calibrate" their ears. In addition, the practical testing environment changes on a random basis to continually challenge the users. These situations allow students to interact at their own speed and therefore customize their learning development.School of Music
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Thompson, Jo. "Pueblo Home: An interactive multimedia CD-ROM on Pueblo architecture." CSUSB ScholarWorks, 1995. https://scholarworks.lib.csusb.edu/etd-project/988.
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Haggerty, Patrick J. "Exploring the future of compact disc-interactive /." Online version of thesis, 1990. http://hdl.handle.net/1850/11374.
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Lee, Michael Sunchul. "An interactive CD-ROM to accompany Haddon Robinson's "Biblical preaching", second edition." Theological Research Exchange Network (TREN) Access this title online, 2005. http://www.tren.com.
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Ladd, Michael J. "An interactive CD-ROM for the instruction of 'classic' sound synthesis methodologies." Virtual Press, 1999. http://liblink.bsu.edu/uhtbin/catkey/1210534.
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The purpose of this study was twofold: first, to collect information relating to `classic' sound synthesis methodologies which have formed the basis of current synthesis methods into one comprehensive report, and secondly, to describe and develop an interactive CD-ROM as a new tool for the instruction of these synthesis methods. The historic trend has been the acquisition of sound synthesis through direct interaction with a particular piece of software or hardware. The intentions of this interactive media are to allow students to form conceptual knowledge, and develop the ability to perceive timbral differences produced by these methods. This environment allows students to interact at their own speed and assist in customizing their learning development.
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Parrish, Mila. "Discover dance CD-ROM for dance education : digital improvisation and interactive multimedia /." The Ohio State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488203857248275.
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Leggett, Michael Graham College of Fine Art & Design UNSW. "Burning the Interface : artists' interactive multimedia 1992 - 1998." Awarded by:University of New South Wales. College of Fine Art & Design, 2000. http://handle.unsw.edu.au/1959.4/19334.
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The thesis describes the development during the 1990s of visual artists' utilisation of computer-based interactive multimedia and the production internationally, with a focus on Australian artists, of artworks on the CD-ROM media format. Earlier parts of the author's research led to the exhibition, 'Burning the Interface < International Artists' CD-ROM>', which he co-curated, opening at the Museum of Contemporary Art, Sydney, in 1996, before touring to Perth, Brisbane, Melbourne and Adelaide. The thesis surveys the range of practice by artists working with digital media and the opportunities for exhibition in the public spaces of museums, galleries and the street, and advances scenarios for correcting the laxity of response by the exhibiting institutions to the vigour with which Australian artists represented their work and ideas at this time in national and international forums. Four published artists' work on CDROM are analysed in detail, and a concluding chapter about 'interactive multimedia' and its usefulness as an art medium to the artist introduces the studio practice component of this MFA submission. This takes the form of a prototype 'experimental' version of an interactive multimedia work on CD-ROM, ('Strangers on the Land') a copy of which is contained in a pocket at the rear of the bound version.
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Pridemore, David H. "Interactive CD-ROM computer tour of the Ball State University Department of Art." Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/961564.
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For my creative thesis project I authored an interactive tour of the Ball State Department of Art. Many underlying factors go into this project. My desire to learn multimedia design, the departments desire to develop a new information tool and having the necessary hardware and software to do such a project were all key to its sucess.In the summer of 1994 I came to Ball State to learn multimedia authoring while getting a master's degree in art. Unknown to me at that time, the department had set a goal of increasing visibility both within and beyond the Ball State community. Faculty members Professor Phil Repp and Professor Christine Paul were collaborating on a promotional identity campaign. From these collaborations grew the idea of a departmental publication to promote the mission and programs of the Department of Art. With the rapid advancement of technology, it seemed appropriate to use computers as part of this promotional campaign.As Professors Paul and Repp researched the possible ways in which computers could be incorporated into this project, many questions remained. Exactly what form should a project like this take and who could do it? Careful discussion and planning also followed over what physical form the project should take (i.e. video tape, a computer disk, or printed material). Eventually the decision was made that an interactive tour of the Department of Art on CD-ROM was the most appropriate solution. For the amount of information that needed to be included and to engage the end user in a dynamic, interactive way, this medium was also the most logical.My decision to return to school coincides perfectly with the departments needs. Professor Paul’s and Professor Repp’s collaboration led to the conclusion that a third person would be needed. Someone who was already literate in advanced computer graphics and had the desire for such an undertaking. Therefore, my goals of advancing my understanding of Macintosh based digital imagery learning multimedia are significant on two levels; my career as a teacher and a professional artist would realize significant gains and this project is an outstanding addition to my portfolio.For the past several years, the primary area of artistic study for me has been in the area of computer graphics and I came to Ball State last summer with some very specific goals. One of them being to learn Macromedia Director (the authoring package I used to create the project). Director is nationally recognized by professionals in this field as the top program for this type of work. Therefore, this was both an opportunity to reach personal goals and to create a thesis project that could be used as an important part of the Department of Arts identity campaign. My thesis project is the result of my own goals and the Department of Arts goals to utilize cutting edge technology for designing innovative computer programs.I’m sure at the onset of this project that I did not understand the full magnitude of an undertaking such as this. However, it is very rewarding to look back and see both how far I’ve come personally and how the piece has progressed into a dynamic information tool.Department of Art
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Kirby, Chad A. "The modern trombone CD-ROM : a digital edition of a standard reference /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/11398.
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Terriah, Sean C. "A survey of networked and compact disc technologies and applications for interactive music systems." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23362.
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The proliferation of the Internet and the convergence of telecommunication, computing, compact disc, and media technologies have paved the infrastructure for new interactive music environments. It is essential for those in the field of new media to be aware of the ramifications of these developments. Furthermore, there has been a growing interest among new media developers to provide the tools necessary to allow an end-user to take an active role in the outcome of an interactive multimedia presentation. This concept introduces the notion of end-user as artist and collaborator. This concept introduces the notion of end-user as artist and collaborator. This thesis investigates the diverse applications and technologies that have emerged through cross-disciplinary collaboration and how they may be used to specify and design innovative musical environments.
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Fedak, Beata. "Interactive learning and multimedia in education with special focus on the framework design of an interactive CD-ROM, light and architecture." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ39650.pdf.
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Engel, Jim, and Jim Menas. "Telemetry Course." International Foundation for Telemetering, 1995. http://hdl.handle.net/10150/611494.
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International Telemetering Conference Proceedings / October 30-November 02, 1995 / Riviera Hotel, Las Vegas, NevadaThis paper presents a new multimedia CD ROM course on Telemetry that has is just being developed for DoD by the Defense Test and Evaluation Professional Institute (DTEPI). The paper will discuss the Commercial Off the Shelf (COTS) software packages that were used to develop the course. It will discuss the methodology used to develop the course and lessons learned in its development. During the presentation of this paper a computer and VGA projector will be used to show some of the material in the course. This is the second CD ROM course developed by DTEPI, the first one was on Time, Space-Position Information (TSPI). The TSPI course has been completed, passed Beta testing at most of the National Ranges, and has been released. About 800 CD ROM disks have been distributed to the Ranges and other qualified users. The Telemetry course will be similarly completed and distributed. The course is intended to be an introduction to the subject of telemetry for use by engineering professionals just entering the workforce, by professionals cross training into T&E, and by others with a need or desire to understand telemetry. The value of developing an interactive course using audio narration, animations, as well as still pictures and video of actual instrumentation and equipment cannot be overemphasized. This multimedia environment makes the explanation of concepts like an optical encoder easily understandable as the student can "see" a simulation of the encoder in operation. The course is designed to be self paced with students controlling their own progress and choosing the topics they want to cover. The student also has the option to print a hard copy of the page narration or read them on screen. The course facilitates the rapid learning of the jargon of telemetry, all the essential acronyms, the way telemetry systems work, what they look like, and many of the limitations of telemetering systems. The course covers the History of Telemetering, Telemetry Subsystems, Range Applications, Telemetry Schemes, Theory of Operation, Telemetry Processing Systems, Testing Telemetry Systems, and other Miscellaneous Topics. It will afford the student a lot of insight into telemetry without the mathematics and detail required of a telemetry design engineer.
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Randall, Robert 1948. "Access writing written segment and documentation accompanying the multi-media research project submitted on a series of four CD ROMs." Monash University, Dept. of Fine Arts, 1998. http://arrow.monash.edu.au/hdl/1959.1/7634.
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Bertrand, Jose Antonio 1948. "The design and development of an interactive high school art education CD-ROM: The Retablo of Ciudad Rodrigo." Thesis, The University of Arizona, 1995. http://hdl.handle.net/10150/291873.
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This paper is a first-person account of the design and development of an interactive High School art education CD-ROM for the University of Arizona Museum, entitled The Retablo of Ciudad Rodrigo CD-ROM. Based on the learning theories of Donald Arnstine and the work of Dr. Dwaine Greer within the framework of Discipline Based Art Education (DBAE), this report provides an account of how art educators and computer theoreticians and programmers worked together on a joint project involving the use of computer CD-ROM technology within art education. Several solutions are discussed in addition to the desktop reference materials included in this thesis.
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Morrissey, Mairead M. "Development and evaluation of an interactive CD-ROM for the promotion of nutrition education in second level schools." Thesis, University of Ulster, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.399061.
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Young, Shwu-ching. "A study of learners' interactions with and perceptions of a CD- rom-based instructional program on interactive writing /." The Ohio State University, 1996. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487940665435521.
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Clayman, Jill F. "An investigation into the development of an interactive archival catalog of art within the Rochester Institute of Technology /." Online version of thesis, 1994. http://hdl.handle.net/1850/12190.
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Miller, Mary Guy. "An in-depth descriptive case study of the development of 5 A Day Adventures, the CD-ROM." Diss., This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-10032007-172032/.
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Maciel, Hagar Ceriane Costa Corsini. "CRIAÇÃO DE CD MULTIMÍDIA ENFOCANDO CONCEITOS E PROCESSOS PRODUTIVOS DE BIODIESEL NO BRASIL. MG." Universidade Jose do Rosario Vellano, 2010. http://tede2.unifenas.br:8080/jspui/handle/jspui/43.
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Previous issue date: 2010-06-22With the current major development in computer science, new methodologies have been used to improve the process of information dissemination. A perspective that cannot be ignored is the question of how to use these technologies to improve the teaching and learning process. One of these methodologies is the multimedia. This study, conducted in the laboratories of the Federal Institute of Informatics Sul de Minas, Campus Machado, MG, and in the José Rosário Vellano University, Campus Alfenas, MG, aims at demonstrating that the use of multimedia techniques, in parallel with all the resources it aggregates, may be a usefull and efficient tool, planned and developed to be a practical source of information collected in a sigle application of study on biodiesel. The Menu strategy, was used for navigation, where one chooses the option of acessing the contents without having to obey any predetermined order. Thus the users will free to access the contents in a dynamic, interactive and pleasant way, according to their interest or need, using audiovisual resources of easy operation and assimilation. The software was available for some students and teachers to evaluate the usability, readability, learning, teaching, multimedia elements, layout and interactivity. In general the software was assessed and satisfactorily met the user s expectations. In a scale of 2-10 points, for all variables studied, the average was above than 8.5 points. The CD-ROM of the biodiesel multimedia is a didactic material developed for educators and high school and college students.
Atualmente, com a grande evolução na informática, novas metodologias vêm sendo utilizadas para melhorar o processo de disseminação das informações. Uma perspectiva que não pode mais ser ignorada é a questão de como utilizar tais tecnologias para melhorar os processos de ensino e aprendizagem. Uma dessas metodologias é o que se chama de multimídia. O presente trabalho pretende demonstrar que a utilização de técnicas multimídia, paralelamente com todos os recursos a ela agregados, poderá ser uma ferramenta útil, eficaz, que foi planejada e desenvolvida para ser uma fonte prática de informações reunidas em uma única aplicação de estudo sobre o tema Biodiesel. O trabalho foi desenvolvido nos Laboratórios de Informática do Instituto Federal Sul de Minas , campus Machado MG, e da Universidade José do Rosário Vellano, campus Alfenas MG. Para navegação foi usada a estratégia de Menu, onde se escolhe a opção de acessar o conteúdo, não obedecendo a nenhuma ordem pré-determinada. Com isso os usuários não se sentirão aprisionados, pois o conteúdo é acessado de forma dinâmica, interativa e prazerosa, de acordo com o seu interesse ou necessidade, utilizando-se de recursos audiovisuais, de fácil operação e assimilação. O software foi disponibilizado para alguns alunos e professores para avaliação quanto à usabilidade, legibilidade, aprendizado, didática, elementos multimídia, leiaute e interatividade. De maneira geral, o software foi avaliado satisfatoriamente e atendeu às expectativas dos usuários. Em uma escala de notas de 2 a 10 pontos, para todas as variáveis estudadas, a média foi superior a 8,5 pontos. O CD-ROM do multimídia Biodiesel é material didático para educadores e alunos do ensino médio e superior.
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Sylaidis, Peter. "Multi media applications in medical education : evaluation of an interactive CD-ROM on practical skin wound management for medical undergraduate learning /." Title page, contents and summary only, 1999. http://web4.library.adelaide.edu.au/theses/09MS/09mss984.pdf.
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Fleming, Robert M. III. "The Seeds of Sustainability: An Interactive CD Rom Exploring the Role of Architects, Planners and Designers in Achieving a Sustainable Future." Thesis, Virginia Tech, 1996. http://hdl.handle.net/10919/37002.
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The generally accepted definition of the term sustainability is: meeting the
needs of the present without compromising the needs of future
generations to meet their own needs. While that def-inition helps to
provide a general framework within which to understand the term, it does
not begin to explain the more specific initiatives, ideas, strategies and
design concepts necessary for an architect, designer or planner to
contribute to the process of achieving a sustainable future. As a result, the
term sustainability is often misunderstood or even mistrusted within the
design professions. The goal of this thesis is to begin the process of
generating a comprehensive understanding of the term sustainability
within the design community. It asks the question: what does it mean,
specifically, and in terms of concrete design strategies for architects,
designers and planners to design for sustainability? In that sense the final
product is intended to be educational and is designed to communicate
information in a visual format that the design community can not only
relate to, but be engaged by. The information is presented in a non-linear
structure to allow dynamic connections between related pieces of
information and between design strategies that solve problems within
seemingly different areas. Ultimately, the final product is intended to
become a CD rom with dynamic links between information within the
presentation and to the Internet.Master of Architecture
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Chau, Katie. "Architecture + hypermedia, a didactic approach to exploring architecture as both content and method in a hypermedia environment; prototype project, an interactive multimedia CD-ROM on exploring sacred Tibetan architecture." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0021/MQ54234.pdf.
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Mouli, Richard. "Un modèle d'animation comportementale fondé sur le concept de personnage." Toulouse 3, 1994. http://www.theses.fr/1994TOU30192.
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Le but de l'animation en synthese d'images est de definir des concepts et des outils pour permettre a l'utilisateur de produire des sequences animees. Les travaux que nous presentons s'inscrivent dans le domaine de la problematique liee a la definition des mouvements des objets mis en scene. L'etude que nous avons realisee porte sur un modele de haut niveau. Les objectifs qui ont guides cette recherche sont la conception et la realisation d'un systeme capable d'animer un nombre important d'objets et de prendre en compte les interactions qu'ils subissent. Nous introduisons pour cela le concept de personnage auquel nous associons un comportement. Un personnage est un objet autonome, actif dont l'evolution dans le temps est le produit de l'evaluation de son comportement en fonction de la connaissance qu'il possede sur son environnement local. Nous decomposons l'ensemble des personnages en trois sous-classes (les personnages acomportementaux, reflexes et intelligents) selon le niveau de comportement qui leur est associe. Les personnages peuvent n'etre soumis seulement aux lois de la physique, reagir automatiquement a des situations simples ou utiliser un raisonnement, fonde sur des regles et un moteur d'inference (comme en prolog) pour analyser la situation dans laquelle ils se trouvent pour decider des actions a realiser. Dans les deux derniers cas, ils utilisent un ensemble de capteurs qui leur permettent d'acquerir des informations sur l'environnement et de determiner la situation presente. Nous introduisons le concept de capteur bati sur un mecanisme actif d'interrogation. Nous presentons aussi des solutions dans le domaine de la coherence d'animation. Plus particulierement, nous introduisons une nouvelle approche pour l'echantillonnage de la dimension temporelle. Grace au systeme realise en langage eiffel sur stations de travail unix dans le cadre de cette these, nous avons produit les sequences animees
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Maitre, Blandine. "Etude de la molécule CD1e et de son trafic intracellulaire." Université Louis Pasteur (Strasbourg) (1971-2008), 2004. http://www.theses.fr/2004STR13164.
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Au sein de la famille des molécules présentatrices d'antigènes CD1, la molécule CD1e est très particulière. Le CD1e est en effet localisé exclusivement de façon intracellulaire et existe, sous une forme associée à la membrane, dans le réticulum endoplasmique et les compartiments golgiens et sous une forme soluble, dans les endosomes. Nous avons étudié d'une part la localisation du CD1e dans les cellules dendritiques (CDs) immatures et en cours de maturation. Dans les CDs immatures, le CD1e est principalement localisé dans le Golgi mais est aussi détecté dans les compartiments tardifs multilamellaires. Dans les CDs matures, les molécules CD1e sont relocalisées dans les compartiments tardifs matures où se trouvent également le CD1b. D'autre part, un modèle de cellules transfectées HLA-DR+ a été utilisé pour comprendre quelles parties du CD1e sont impliquées dans le contrôle de son trafic et pour connaître les mécanismes cellulaires qui s'y rapportent. Nous avons montré que le domaine cytoplasmique du CD1e joue un rôle important dans la rétention intracellulaire du CD1e au niveau des compartiments golgiens et dans l'adressage du CD1e vers les compartiments tardifs. Sa substitution par celui du CD1b empêche la génération d'une forme soluble. Ces résultats suggèrent que le trafic du CD1e est destiné à l'obtention d'une forme soluble et que le CD1b est exclu de ce trajet. De plus, en absence des complexes AP-1 et AP-3, le CD1e gagne toujours les endosomes tardifs mais la déficience en AP-1 empêche son clivage. Enfin, nous avons montré que le CD1e possède un propeptide, clivé dans les endosomes. Ces résultats suggèrent que le CD1e acquiert ses fonctions biologiques dans ces compartiments. Ces travaux pourront être poursuivis sur le plan fonctionnel au moyen de tests de présentation antigènique, dans le cadre d'un travail collaboratif, dont les premiers résultats mettent en évidence un rôle du CD1e pour la présentation de certains glycolipides par le CD1bCD1e belongs to the family of antigenic presenting molecules CD1 but CD1e is very particular. Indeed, CD1e remains surprisingly intracellular, accumulating mainly in the Golgi compartments of immature dendritic cells (DCs) into a complete transmembrane associated form and in the late endosomes of mature DCs. In the latter compartments, CD1e is cleaved and becomes soluble due to a cleavage between the luminal and transmembrane domains. We studied the distribution of CD1e in immature and maturing DCs. In immature DCs, CD1e accumulates in Golgi compartments, is not expressed on the plasma membrane but reaches CD1b+ late multilamellar compartments. During maturation, CD1e molecules accumulate in mature endosomes CD1b+. Its cytoplasmic domain does not contain known targeting motifs. CD1e reaches late endosomes in AP-1 and AP-3 deficient cell lines. Interestingly, the cleavage of CD1e occurs in AP-3-, but not in AP-1- cells. Deletion and substitution mutants were engineered and expressed in an HLA-DR+ melanoma cell line. Experiments showed that different parts of the cytoplasmic tail control the localization of CD1e in the golgi compartments and its absence on the cell surface, facilitating the routing of CD1e to late endosomes where it is cleaved. The replacement of the cytoplasmic domain of CD1e by that of CD1b blocked its cleavage. Thus, CD1e reaches late endosomes using a cellular pathway not shared by CD1b. Finally, we show that CD1e contains a propeptide which is cleaved in endosomes. Our data strongly suggest that the function of the intracellular transport of CD1e is the generation of soluble endosomal molecules, which might play a role in the presentation of antigens by other CD1 molecules transiting in CD1e+ endosomes
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Hermann, Patrice. "Recherche du ligand du CD40 : étude du rôle de son interaction avec le CD40 dans la réponse lymphocytaire B." Lyon 1, 1995. http://www.theses.fr/1995LYO1T120.
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Faïf, Anne-Marie Garance. "Mesure de la section efficace totalement leptonique et des rapports d'embranchement des bosons W+- dans le cadre de l'expérience ALEPH à LEP2." Université Joseph Fourier (Grenoble), 1998. http://www.theses.fr/1998GRE10248.
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Ce travail de these s'inscrit dans le cadre des mesures de precision effectuees a lep2. Les bosons w# y sont produits par paires dans les collisions e#+e#. Les etats finals totalement leptoniques (l$$$$l) representent 10. 5% de ces desintegrations. Ce canal s'ajoute aux canaux hadroniques et semi-leptoniques pour contraindre le modele standard. Les etats finals l$$$$l, caracterises par deux leptons charges energiques et acolineaires ainsi qu'une importante impulsion transverse manquante, ont ete selectionnes parmi les donnees enregistrees par l'experience aleph entre 1996 et 1998. Les sections efficaces totalement leptoniques correspondantes ont ete calculees afin de determiner les rapports d'embranchement du boson w. Contrairement aux mesures effectuees au tevatron, les rapports d'embranchement sont determines sans contrainte theorique. La precision obtenue au lep sur les rapports d'embranchement leptoniques individuels et le rapport d'embranchement hadronique du w est la meilleure a ce jour. Les resultats obtenus ne montrent pas de deviation significative par rapport aux predictions du modele standard.
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SERRU, VALERIE. "Etude de l'organisation et de la fonction des complexes macro-moleculaires a tetraspanines." Paris 5, 1999. http://www.theses.fr/1999PA05N130.
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Sala, Fernanda Angélica. "Estudo da interação entre domínios C-terminais de septinas humanas: implicação na formação e estabilidade do filamento." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/75/75133/tde-10082015-140053/.
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Septinas compreendem uma conservada família de proteínas de ligação a nucleotídeo de guanina e formação de heterofilamentos. Em termos estruturais, elas possuem uma organização comum: um domínio GTPase central, uma região N-terminal e um domínio C-terminal, este último é predito para formar estruturas em coiled coil. Atualmente, o heterocomplexo de septinas humanas (SEPT2/SEPT6/SEPT7) mais bem caracterizado revela a importância do domínio GTPase na formação do filamento, todavia a ausência de densidade eletrônica para os domínios C-terminais faz com que sua função permaneça obscura. Estudos com septinas de mamíferos, e de outros organismos como C. elegans e S. cerevisea sugerem que alguns grupos de septinas (por exemplo, II e IV em mamíferos) interagem através de seus domínios C-terminais, e estes poderiam atuar de modo determinante para a montagem correta do filamento. Assim, o presente projeto objetivou estudar a afinidade homo/heterotípicas para os domínios C-terminais das septinas humanas dos grupos II (SEPT6C/8C/10C/11C) e IV (SEPT7C), investigando se esses domínios contribuem para preferência das septinas interagirem com proteínas de grupos distintos durante a formação do heterofilamento. Os domínios C-terminais foram expressos em E. coli e purificados. Foram conduzidos estudos de ultracentrifugação analítica e espectropolarimetria de dicroísmo circular, que permitiram identificar maior afinidade e estabilidade da associação heterotípica comparada à homotípica. Foram obtidas constantes de dissociação aparente para homodímeros em torno de baixo µM, enquanto que para heterodímeros os dados já existentes no grupo revelaram constante de dissociação na ordem de nM. Para entender os fatores no nível atômico responsáveis pela significativa predileção na interação entre os domínios C-terminais dos grupos II e IV foram realizados estudos utilizando modelagem e análise das sequências primárias. As análises sugerem a presença de um alto número de resíduos carregados na posição a do coiled coil como responsável pela seletividade. Consequentemente, o heterodímero seria favorecido em virtude do menor efeito repulsivo proveniente do intercalamento dos resíduos carregados em a. Desse modo, os resultados indicaram a atuação decisiva ou cooperativa dos domínios C-terminais na organização preferencial das septinas durante a formação do filamento, favorecendo a interface NC entre septinas dos grupos II e IV.Septins comprise a conserved protein family that binds guanidine nucleotide and forms heterofilaments. In structural terms they have a common organization: a central GTPase domain, a N-terminal domain and a C-terminal domain, this last one is predicted to form coiled coil structures. Currently, the human septin heterocomplex best characterized (SEPT2/SEPT6/SEPT7) reveals the importance of the GTPase domain in filament assembly, however the absence of electron density for the C-terminal domains makes its function still unknown. Studies with mammals septins, and of others organisms like C. elegans and S. cerevisea suggests that some septins groups (e.g. II e IV in mammals) interact via its C-terminal domains and this could act in a determinative way to correct filament assembly. In this way, this project aimed to study the homo/heterotypical affinity for the C-terminal domains of human septins belonging to groups II (SEPT6C/8C/10C/11C) e IV (SEPT7C), investigating whether this domain contributes with the preference of septins to interact with proteins of different groups during assembly of the heterofilament. The C-terminal domains were expressed in E. coli and purificated. It was carried out studies using analytical ultracentrifugation and circular dichroism spectropolarimetry tecniques which allowed identification of major affinity and stability in the heterotypical association compared to homotypical. It was measured apparent dissociation constants for homodimers of low µM range while for heterodimers our group\'s data shows dissociation constants in the nM range. To understand at atomic level the factors responsible for this significant preference in the C-terminal domains interaction between groups II and IV was performed molecular modelling studies and analysis of the primary sequence. These analysis suggests the presence of a high number of charged residues in position a of the coiled coil as responsible for selectivity. Consequently, the heterodimer would be therefore favoured because of the minor repulsive effect coming from the staggered of charged residues in a. Thus, these results indicate the crucial or cooperative action of C-terminal domains in preferential organization of septins during filament assembly, favouring the NC interface between septins of groups II and IV.
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Kemayo, Koumkoua Patricia. "Structural characterisation of highly specific membrane protein-lipid interactions involved in cellular function." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAF055/document.
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Les membranes cellulaires sont des systèmes complexes composés de lipides variés qui interagissent avec les protéines pour accomplir une fonction. Leur adressage spécifique dans la cellule est crucial pour le fonctionnement cellulaire. Les vésicules COP (coat protein) sont impliquées dans leur transport dans les premières étapes de la voie de sécrétion. Récemment, une interaction très spécifique a été identifiée entre le domaine transmembranaire de la protéine p24 (p24TMD) abondante dans la membrane des vésicules COP et la sphingomyéline C18:0. Cette spécificité a été identifiée dans le cas d’interaction protéine-protéine et protéine-acide nucléique comme impliquée dans la régulation de fonctions cellulaires, C’est pourquoi nous avons décidé d'étudier sur cette interaction. A cet effet, le p24TMD a été obtenu par synthèse chimique et sa structure étudiée par RMN du solide en présence de la sphingomyèline avec pour but ultime de comprendre la fonctionCell membranes are complex systems composed of variety of lipids that interacts with proteins to trigger cellular function. The delivery of these lipids to the right compartment is crucial for cells to work efficiently. The coat protein (COP) complex vesicles are involved in lipids traffic in the early stages of the secretory pathway. Recently, a highly specific interaction has been found between the transmembrane domain of p24 protein (p24TMD) abundant in COPI membrane and sphingomyelin C18:0. As such highly specific interaction have been reported for protein-protein and protein-nucleic acid interactions to be involved in regulation of cell functions, we decide to investigate this specific interaction. The p24TMD was obtained chemically and investigated by solid state NMR in presence of sphingomyelin with the ultimately goal to understand the function behind
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Clift, Martin James David. "Quantum dots : an investigation into how differing surface characteristics affect their interaction with macrophages in vitro." Thesis, Edinburgh Napier University, 2009. http://researchrepository.napier.ac.uk/Output/2557.
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Quantum dots (QDs) are potentially advantageous tools for both diagnostics and therapeutics due to their light emitting characteristics. The impact of QDs on biological systems however, is not fully understood. The aim of this project therefore, was to investigate the interaction of a series of different surface modifies QDs with macrophages and their subsequent toxicity. CdTe/CdSe (core), ZnS (shell) QDs with either an organic, COOH or NH2 polyethylene glycol (PEG) surface coatings were used. Fluorescent COOH polystyrene beads (PBs) at (Ø) 20nm and 200nm were also studied. J774.A1 murine ‘macrophage-like' cells were treated for two hours with QDs (40nM) of PBs ($50μg.ml^{-1}$) in the presence of 10% FCS prior to assessment of cellular uptake via confocal microscopy and flow cytometry. COOH and $NH_{2}$ (PEG) QDs, as well as 20nm and 200nm PBs entered macrophages within 30 minutes, and were found to locate within endosomes, lysosomes and the mitochondria. T.E.M. also illustrated particles, including organic QDs, to be present inside J774.A1 cells within membrane- bound vesicles at two hours. Organic QDs were unable to be visualised via fixed cell confocal microscopy. Live cell confocal microscopy (without 10% FCS) did suggest however, that organic QDs entered cells in low quantities up to 30 minutes, after which fluorescence declined. Particle toxicity was determined over 48 hours via the MTT, LDH and GSH assays, as well as via assessment of their potential to produce the pro-inflammatory cytokine (TNF-α) and effect cytosolic $Ca^{2+}$ signalling in the J774.A1 cells. Organic QDs were found to be highly toxic at all time points and concentrations used. Both COOH and $NH_{2 }$ (PEG) QDs induced significant (p<0.0001) cytotoxicity (MTT and LDH assays) at 80nM after 48 hours, as well as significant (p<0.01) GSH depletion over 24 hours at all doses, as well as increasing the level of cytosolic $Ca^{2+}$ at 40nM when assessed over 30 minutes. Organic and NH2 (PEG) QDs were found to significantly increase TNF-α production after 24 hours at 80nM. The findings of this study demonstrate that QDs differ in their uptake by macrophages according to their surface coating, with the organic surface coated QDs being the most toxic. At sub-lethal concentrations, in the presence of 10% FCS, the COOH and $NH_{2}$ (PEG) QDs are taken up resulting in GSH depletion and modulated $Ca^{2+}$ signalling, with $NH_{2}$ (PEG) QDs and organic QDs only eliciting limited TNF-α production. Interestingly however, despite these observations, QD surface coating does not affect the intracellular fate of these NPs, with all of the different surface coated QDs observed to be present in endosomes, lysosomes and the mitochondria within J774.A1 macrophage cells. Therefore, in conclusion, the surface coating of QDs plays a significant role in their interaction with macrophages, their uptake and their subsequent toxicity.
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Al-Khouri, Sa'ib. "UV/Vis and CD Spectral Studies of the Interaction between Pinacyanol Chloride and Alginates, g-Cyclodextrin, and Aerosol-OT." Gerhard-Mercator-Universitaet Duisburg, 2003. http://www.ub.uni-duisburg.de/ETD-db/theses/available/duett-05062003-112253/.
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A quantitative study of the aggregation behaviour of the cationic cyanine dye pinacyanol chloride in aqueous solution and in the presence of various organic matrices (anionic alginates, anionic Aerosol-OT and g-cyclodextrin) is presented based on UV/Visible absorption and Circular Dichroism (CD) spectroscopy. The spectral data have been analysed using derivative spectra and a program (PeakFit) to model the mixtures of different absorbing species with strongly overlapping absorption bands. The structures of the aggregates have been analysed and discussed in terms of qualitative (H- vs. J-type aggregation) and quantitative arguments based on a program (OSCI) for the calculation of oscillator and rotatory strengths assuming an exciton-like delocalisation of molecular excitations through the entire aggregate. Models for the various complexes of the dye in the presence of various organic matrices have been proposed
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EliÌdoÌttir, JoÌrunn. "How can we empower children to improve the quality of their learning experiences through interaction with a CD ROM?" Thesis, Coventry University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247966.
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Huffman, Celia A. "Student Interactions With CD-ROM Storybooks: A Look At Potential Relationships Between Multiple Intelligence Strengths And Levels Of Interaction." Kent State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=kent1332695988.
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Gallin-Martel, Marie-Laure. "Étude de la désintégration B-D(étoile)+D(étoile)-X dans l'expérience DELPHI au LEP du CERN." Grenoble 1, 1996. http://www.theses.fr/1996GRE10053.
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Le travail presente dans cette these consiste en la mise en evidence des desintegrations inclusives des hadrons beaux b en deux mesons charmes d excites de signe oppose a l'aide du detecteur delphi du lep. Une methode de reconstruction partielle basee sur la masse invariante des deux pions celibataires produits dans la desintegration des mesons d excites et charges en mesons d neutres a ete elaboree. Les donnees enregistrees entre 1991 et 1994 ont ete analysees. Cette etude a permis d'isoler des evenements dans trois canaux de desintegration du meson d neutre. La recherche de kaons charges additionnels a entraine la reduction du bruit de fond combinatoire. La valeur du rapport d'embranchement de la desintegration inclusive des hadrons b en deux mesons d excites de signe oppose est estimee a 1. 2 pour-cent plus ou moins 0. 3 pour-cent
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Ng, Lee-Luan, and n/a. "The influence of cognitive styles on the interaction with a language instruction CD-ROM : a case study of Malaysian ESL learners." University of Otago. Department of English, 2005. http://adt.otago.ac.nz./public/adt-NZDU20061025.115945.
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Computer technology has been introduced into the field of language education for more than three decades. However, how individual learners interact with language learning multimedia programmes in different ways is not yet understood. A possible explanation is related to the learners� variables. The purpose of this study is to investigate the impact of cognitive style, one of these variables, on the language learning process. The participants of this study were a group of ESL university students from Malaysia, who interacted with a language instruction CD-ROM. This study focuses on investigating the interaction process from the students� perspective.
Riding�s Cognitive Style Analysis, a computer software that measures individual�s cognitive style, was used to assist in identifying and categorizing students� cognitive style. Thirty students� cognitive style was measured before each student spent an average of three hours interacting with a language instruction CD-ROM. This study adopts the case study approach. The primary method of collecting data involved semi-structured interviews with students that focused on their experiences and preferences while interacting with language instruction CD-ROM. Factors that affected the interaction process were then examined to detect whether they were linked to the differences of students� cognitive orientation.
The findings of this study reflect the mixed results that previous researchers have found regarding the effect of cognitive style on students� language learning experiences. Although responses toward the content, graphics and vocabulary items did partially reflect their cognitive orientation, cognitive style was not the only factor that influenced these students� behaviours or preferences as they interacted with the language instruction CD-ROM. The study found factors that were not linked to the students� cognitive styles, yet these also influenced the interaction process. These non-cognitive-style factors consisted of students� language learning motivation, social presence, and their conception of language learning and cultural influence, which resulted in teacher-centeredness. As a result of these findings, theoretical, research and pedagogical implications are discussed. Recommendations and limitations of the study are also presented.
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Ng, Lee-Luan. "The influence of cognitive styles on the interaction with a language instruction CD-ROM a case study of Malaysian ESL learners /." Online version, 2005. http://bibpurl.oclc.org/web/30836.
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Haury, Axel. "Hétérostructures à puits quantique Cd(Mn)Te/CdMgZnTe dopées pgaz de trous et phase ferromagnétique." Université Joseph Fourier (Grenoble), 1997. http://www.theses.fr/1997GRE10205.
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Nous presentons la premiere mise en evidence d'une phase ferromagnetique dans des heterostructures a puits quantique semimagnetique cdmnte/cdmgznte dopees p par modulation (azote) dans les barrieres. Dans de tels echantillons, le transfert de trous des barrieres dans le puits cree un gaz de trous quasi-bidimensionnel dans la couche de cdmnte. Pour optimiser les conditions de dopage, une etude preliminaire a ete menee sur une serie d'echantillons a puits quantiques non magnetiques cdte/cdmgznte montrant que, dans des puits de 80 a, le gaz de trous devient metallique pour des densites superieures a 1. 5 x 10#1#1 cm#-#2. En outre, ces echantillons nous ont permis d'observer les deux types d'excitons charges x#+ et x#-. Dans les echantillons a puits semimagnetique cdmnte, l'interaction d'echange entre les ions magnetiques mn#+#+ et le gaz de trous en phase metallique ( 2 x 10#1#1 cm#-#2) cree un couplage ferromagnetique du type rkky entre les spins localises des ions mn#+#+. Dans des puits d'une largeur de 80 a et pour des concentrations de mn d'environ 3%, l'interaction ferromagnetique induite par le systeme de trous bidimensionnel devient superieure au couplage antiferromagnetique de superechange entre les ions magnetiques mn#+#+. Ceci se traduit par une transition de phase ferromagnetique a la temperature critique t#c d'environ 2 k. La phase paramagnetique au dessus de t#c est caracterisee par une augmentation spectaculaire de la susceptibilite magnetique.
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Mennechet, Franck. "Homéostasie intestinale après infection par T. Gondii : interaction entre les lymphocytes de la lamina propria (LPLs) et les lymphocytes intraépithéliaux (LIEs)." Tours, 2002. http://www.theses.fr/2002TOUR3805.
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Lors de l'infection orale par le parasite T. Gondii, la réponse immunitaire intestinale générée fait intervenir des éléments effecteurs initiateurs et régulateurs de l'inflammation, qui interagissent entre eux à la fois pour combattre l'infection et contrôler la réponse inflammatoire. L'infection conduit à la libération massive de chimiokines par les entérocytes, permettant le recrutement accru des cellules inflammatoires dans l'intestin. Les lymphocytes CD4+ de la lamina proprié en synergie avec les entérocytes ont une fonction pro-inflammatoire Th1 intense car ils sécrètent de grande quantité de cytokines inductrices de l'inflammation, tel que l'IFN-y et le TNF-α. L'interaction CD40-CD154 et la production d'IL-12 sont indispensables à l'initiation de cette réponse immunitaire intestinale. Le développement de la réponse immune tend à supprimer le parasite, tout du moins d'en limiter la prolifération et d'induire son enkystement, phase latente du parasite qui lui permettra également de survivre dans l'hôte. A fin de survivre à sa propre réponse inflammatoire, l'hôte met en jeu une série de mécanismes modulateurs qui participent majoritairement au retour à l'homéostasie. Les lymphocytes intraépithéliaux ont une activité régulatrice puissante et modulent l'inflammation en agissant sur l'activation des celllules T CD4+ de la lamina proprié et des entérocytes par la production massive de TGF-β. Chez la souris C57BL/6 cette réponse immunitaire est mal contrôlée et conduit à son débordement, puis au développement d'une pathologie intestinale létale dans une période de 7 à 9 jours après infection. Par la localisation des lésions, son aspect immunologique, infectieux et génétique, la pathologie générée par le parasite T. Gondii chez la souris C57BL/6 partage de nombreuses caractéristiques avec la maladie de Crohn chez l'homme. Ce modèle d'iléite expérimentale pourrait ainsi constituer un outil indispensable pour l'étude de cette pathologie humaineOral infection with Toxoplasma gondii (T. Gondii) triggers a robust intestinal immune response associated with a number of well-described immunologic events. The immune response involves inflammatory and regulatory components, which control both the parasite dissemination and the inflammatory process. In synergy with intestinal epithelial cells, lamina proprié CD4+ T cells are an important componant of this intestinal process. CD40-CD154 interaction and IL-12 expression appear to be essential for the induction of this intestinal immune response. The inflammatory process limits the parasite proliferation and leads to cyst formation into the host tissues. In order to survive against its own inflammatory response, the host generates several intestinal immunoregulatory events, which control the intestinal homostasis. TGF-β-producing intraepithelial lymphocytes modulate the inflammation by controlling both la mina propria CD4+ T cell and epithelial cell activation. At day 7 after oral infection with T. Gondii cysts, unmanipulated C57BL/6 mice fail to regulate this inflammatory process and evelop an acute lethal ileitis. It is the collapse of gut homeostasis that is also the major cause of the chronic intestinal inflammatory bowel disease (IBD) in human. One hypothesis for the pathogenesis of IBD is thas disease is due to an abnormal response of the gut immune system to some antigens from the normal mucosal microflora. Thus, this pattern-driven ileitis model has many histological and immunological characteristics of human Crohn's disease and may be particularly useful for a better understanding of the immunopathogenesis of this human pathology
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Esposito, Christina. "Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0020/MQ47796.pdf.
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OLIVE, ENRICK. "Thermodynamique a l'equilibre et dynamique chaotique dans des systemes de boussoles en interaction dipolaire." Université Joseph Fourier (Grenoble), 1996. http://www.theses.fr/1996GRE10268.
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Dans le cadre d'etude de systemes 2d ou les interactions dipolaires jouent un role important, nous avons construit des reseaux de boussoles sur lesquels les premieres observations ont montre la grande richesse d'etudes possibles. Les divers comportements observes montrent en effet de nombreuses analogies avec des systemes physiques reels. Deux etudes differentes ont ete menees sur ces systemes, experimentalement et en simulation numerique parallelement. Dans la premiere, on s'interesse aux comportements du reseau carre soumis a des effets de temperature, qui sont analyses par un modele simple dans le cadre de la thermodynamique a l'equilibre. Les effets d'anisotropie, resultant des termes multipolaires, conduisent aux notions de defauts et de longueur de lignes. Ces quantites permettent de mettre en evidence la transition entre une phase ordonnee et une phase desordonnee. Une description detaillee de cette transition permet de definir l'apparition d'une direction privilegiee (directeur) au passage de la temperature critique. On definit alors un parametre d'ordre qui est une mesure du peuplement du directeur. La seconde partie de ce travail s'attache a la dynamique individuelle de quelques boussoles en interaction. Le diagramme de phase des regimes dynamiques observes experimentalement est etabli pour deux boussoles avec de faibles frottements soumises a un champ tournant. Pour une dissipation plus elevee, une etude de la destabilisation des regimes periodiques vers des regimes chaotiques est menee. Les routes vers le chaos sont alors identifiees (cascades de doublements de periode) par des diagrammes de bifurcations, et le chaos est mis en evidence numeriquement par des sections de poincare des attracteurs etranges. Cette etude de la dynamique des boussoles se termine par quelques comportements observes et mesures pour une ligne de dix boussoles en interaction. Un scenario nouveau se dessine alors: l'intermittence. Les perspectives d'une etude plus detaillee sur la dynamique des reseaux sont alors tracees
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Raw, Juliana. "Estudo da interação de líquidos iônicos com proteínas modelo." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-22112016-153329/.
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Líquidos iônicos (LIs) são sais que se encontram no estado líquido em temperaturas menores que 100ºC e que vêm ganhando protagonismo na área chamada química verde, prometendo: substituir solventes nocivos ao meio ambiente, aprimorar componentes eletrônicos, favorecer biocatálises dentre outros. Sua alta estabilidade e baixa toxicidade são frequentemente afirmadas, porém, devem ainda ser melhor investigadas. Com o objetivo de implementar o entendimento da interação dos líquidos iônicos com sistemas de relevância biológica, realizamos um estudo sistemático acerca da interação de 3 diferentes líquidos iônicos anfifílicos de mesma cabeça polar e diferentes caudas carbônicas ([C10mim][Cl], [C12mim][Cl] e [C14mim][Cl]) com 3 diferentes proteínas modelo, através das técnicas de absorção óptica, fluorescência, dicroísmo circular (CD) e espalhamento de raios-X a baixos ângulos (SAXS). Para Tanto, utilizamos as proteínas BSA e HSA (Albuminas de Soro Bovino e Humano, respectivamente) além da lisozima. Observamos a supressão da fluorescência das proteínas em todos os casos analisados, onde a diminuição da intensidade correspondeu a, para as proteínas BSA, HSA e lisozima, respectivamente, (55±3)%, (16.1±0.8)% e (4.1±0.2)%, em presença de 0.6mM de [C14mim][Cl], (38±2)%, (13.2±0.7)% e (0.6±0.1)% em presença de 0.6mM de [C12mim][Cl] e (11.0±0.5)%, (9.2±0.5)% e (0.0±0.1)% em presença de 0.6mM de [C10mim][Cl]. Os espectros de absorbância e fluorescência de todos os sistemas nos indicam uma interação de contato entre as proteínas e os líquidos iônicos. Constatamos também o deslocamento do pico de fluorescência, das proteínas BSA e HSA, para menores comprimentos de onda (blue-shift), na medida em que a concentração de LI era aumentada. O máximo deslocamento () alcançado correspondeu a (21±1)nm para ambas albuminas, enquanto que a lisozima não apresentou deslocamento significativo. O blue-shift pode ser explicado pela aproximação das cadeias carbônicas e formações de pontes de hidrogênio nas proximidades dos triptofanos. De acordo com a técnica de SAXS, evidenciamos o aumento do raio de giro das proteínas, na medida em que adicionamos LIs. O raio de giro da BSA, da HSA e lisozima em ausência de LI são (29±1)Å, (30±1)Å e (15±1)Å, respectivamente, e passam para (46±1)Å, (44±1)Å e (20±1)Å respectivamente, em presença de 0.6mM de [C14mim][Cl]. As curvas de SAXS também apresentaram o indício da formação de estruturas micelares a partir de uma dada concentração. Além da alteração em sua estrutura terciária, os dados de CD indicam uma leve perda de estrutura secundária de ambas as albuminas (BSA e HSA), passando de 80 para 65% de -hélice em ausência e presença de 0.6mM de [C14mim][Cl], respectivamente. Sugerimos que as interações das proteínas com os líquidos iônicos, embora inicialmente movidas por forças eletroestática, possuem como principal fator o efeito hidrofóbico, portanto quanto maior a cadeia carbônica do LI maior é sua interação com a proteína. Tal interação causa o desenovelamento das proteínas e formação de um complexo e estruturas micelares a altas concentrações de LI. Acreditamos que este trabalho traz novas informações acerca da interação dos LIs com proteínas modelo, indicando sua capacidade de alterar a conformação das mesmas.Ionic liquids (ILs) are salts that are liquid at temperatures smaller than 100 ° C and are gaining prominence in the so-called green chemistry, promising: replace harmful solvents to the environment, improve electronic components, and favor biocatalysis, among others. Its high stability and low toxicity are often asserted; nevertheless, they are ascribed to ILs due to its small volatility. With the aim of improving the understanding of the interaction of ILs with biological relevant systems, we conducted a systematic study of the interaction of three different ionic liquids of the same polar head and different paraffinic tails ([C10mim][Cl], [C12mim][Cl] and [C14mim][Cl]) with three different model proteins, through the techniques of optical absorption, fluorescence, circular dicrhoism (CD) and small angle X-ray scattering (SAXS). To do so, we use BSA and HSA proteins (Bovine Serum Albumin and the Human Serum Albumin, respectively) and lysozyme. We observed fluorescence quenching, of all studied proteins, where the decrease in the fluorescence was (for BSA, HAS and lysozyme, respectively): (55 ± 3)%, (16.1 ± 0.8)% to (4.1 ± 0.2 )% in the presence of 0.6mm [C14mim][Cl], (38 ± 2)%, (13.2 ± 0.7)% to (0.6 ± 0.1)% in the presence of 0.6mm [C12mim][Cl] and ( 11.0 ± 0.5)% (9.2 ± 0.5)% and (0.0 ± 0.1)% in the presence of 0.6mm [C10mim][Cl]. UV-vis absorbance spectra and fluorescence indicate all systems in a contact interaction between proteins and ionic liquids. We also note the shift of the fluorescent peak of BSA and HSA proteins for shorter wavelengths (blue-shift), as the IL content was increased. The maximum shift () achieved corresponded to (21 ± 1) nm for both albumins, whereas no significant displacement was observed for lysozyme. The blue-shift can be explained by the approach of carbon chains and formation of hydrogen bonds in the vicinity of tryptophan. SAXS data indicate an increasing in the proteins radius of gyration value as ILs was added in the solution. The turning radius of BSA, HSA and lysozyme in the absence of IL are (29 ± 1) Å, (30 ± 1) Å and (15 ± 1) Å, respectively, and go to (46 ± 1) Å, ( 44 ± 1) Å and (20 ± 1) Å, respectively, in the presence of 0.6mm [C14mim][Cl]. The SAXS curves also show evidence of the formation of micellar structures from a given concentration. Besides the change in its tertiary structure, the CD data indicates a slight loss of secondary structure of both albumins (BSA and HSA), from 80 to 65% of -helix in the absence and presence of 0.6mm [C14mim][Cl], respectively. We suggest that the interactions of the protein with the ionic liquid, although initially driven by electrostatic forces, have a major factor hydrophobic effect and thus the higher the carbon chain of greater IL is its interaction with the protein. This interaction causes unfolding of the protein and formation of a micellar structures at high concentrations of IL. We believe this work provides new information about the interaction of ILs with model proteins, indicating its ability to alter the conformation of the same.
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TUCOULOU, TACHOUERES REMI. "Modulation spatiale et temporelle d'un faisceau de rayons x par interaction avec des ondes acoustiques de surface." Université Joseph Fourier (Grenoble), 1996. http://www.theses.fr/1996GRE10273.
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La reflexion d'un faisceau x ( 1 a) sur la surface d'un cristal piezoelectrique (linbo#3) excite par des ondes acoustiques s'accompagne de par et d'autre du faisceau reflechi de pics satellites intenses (15% de l'intensite du faisceau direct) dus a la rugosite sinusoidale de la surface. Un phenomene similaire s'observe autour des diffractions de bragg d'une multicouche deposee sur un cristal piezoelectrique dont la surface est parcourue par des ondes acoustiques. Nous avons montre que la position angulaire de ces pics satellites depend de l'angle d'incidence et du rapport des longueurs d'onde x et acoustique et pouvait donc etre modifiee en changeant la frequence acoustique. Ceci nous a conduit a developper une premiere application permettant le balayage spatial d'un faisceau x par variation de la frequence acoustique autour de la frequence de resonance de l'emetteur. Ce balayage a une ou deux dimensions si l'on associe deux deflecteurs acoustiques, peut atteindre 1 mm a une distance de 1 m du cristal avec une sensibilite de l'ordre du nanometre, bien inferieure aux dimensions actuelles des faisceaux (de l'ordre de 1 m#2). Nous avons mene une etude sur l'association d'un systeme electro-acoustique avec une optique focalisante (lentille de bragg-fresnel) pour obtenir des balayages de faisceaux focalises. Les utilisations possibles sont dans la microanalyse, la microscopie x et la lithogravure. La seconde application presentee dans cette these consiste a introduire une pulsation de la structure temporelle de l'onde acoustique afin d'obtenir une modulation temporelle de l'intensite des faisceaux x satellites. Une utilisation interessante est le selecteur haute frequence de pulses synchrotrons realise en synchronisant les pulses acoustiques sur la frequence des paquets d'electrons qui tournent dans l'anneau. Ce filtre temporel elimine 99% des photons indesirables. Ces faisceaux adaptables sont utiles pour les experiences resolues en temps
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Sokona, Sidi Békaye. "A propos de la proportionnalité : avatars d'une situation didactique dans sa transmission à un enseignant et son insertion dans un curriculum existant." Grenoble 1, 1993. http://www.theses.fr/1993GRE10181.
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De nombreux travaux sur la proportionnalite mettent en evidence la preference des eleves pour des procedures scalaires (fondees sur les proprietes d'isomorphisme) aux depens de procedures fonctions (fondees sur le coefficient de proportionnalite) qui sont privilegiees par l'enseignement. L'objectif de ce travail consiste a prevoir les conditions dans lesquelles un enseignement peut faire evoluer les eleves de l'utilisation de procedures scalaires a celle des procedures fonctions tout en donnant du sens aux procedures fonctions. Un micro-processus d'enseignement a ete elabore et experimente fonde sur une analyse theorique prealable de situations a-didactiques permettant une telle evolution. L'etude que nous menons dans cette these porte sur les comportements des eleves dans differentes situations et sur celui de l'enseignant dans l'execution de son role pendant les phases collectives de ce processus. L'analyse des donnees recueillies a permis de reperer les effets limites des phases collectives et le probleme de la devolution des situations relevant d'une part des conditions de travail inhabituelles par rapport a la coutume de la classe et d'autre part des modes d'interventions de l'enseignant. L'experimentation realisee montre donc l'insuffisance de la seule consideration des phases a-didactiques d'un processus et la necessaire prise en compte des conditions de fonctionnement plus large de la classe, en particulier le type d'interactions habituel entre enseignant et eleve
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Al-Khouri, Sa'ib [Verfasser], Volker [Akademischer Betreuer] Buß, and DIETRICH [Akademischer Betreuer] DOEPP. "UV/Vis and CD Spectral Studies of the Interaction between Pinacyanol Chloride and Alginates, γ-Cyclodextrin, and Aerosol-OT / Sa'ib Al-Khouri. Gutachter: Dietrich Döpp. Betreuer: Volker Buß." Duisburg, 2013. http://d-nb.info/1037989910/34.
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Makrinos, George Adam. "Drawing Music, Playing Architecture." Thesis, Virginia Tech, 2005. http://hdl.handle.net/10919/33890.
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Architecture and music share intrinsic meanings generated by a constant stream of metaphors which are forms of poetic transformations.
This thesis sought to challenge the present way an architect-musician makes drawings through the exploration of multimedia possibilities at hand. The drawings are composed using Macromedia Flash MX.
OPEN HOMEPAGE.EXE
To download flash player, click here:
Download flash PlayerMaster of Architecture
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Ferrari, Arnaud. "Calorimétrie électromagnétique et recherche de neutrinos droits de Majorana dans l'expérience ATLAS." Phd thesis, Université Joseph Fourier (Grenoble), 1999. http://tel.archives-ouvertes.fr/tel-00005400.
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Malgré l'accord remarquable entre le modèle standard et les nombreuses mesures de précision effectuées depuis quelques décennies, la physique des particules a encore bien des mystères à éclaircir. L'un deux concerne la violation de la parité et la masse des neutrinos. Pour résoudre cette énigme, le modèle symétrique et le mécanisme du See-Saw prédisent l'existence de nouveaux bosons de jauge (W$_R$ et Z') et de neutrinos droits de Majorana N$_l$. Si ces nouvelles particules ont des masses voisines de quelques TeV/c2, le LHC et son détecteur ATLAS devraient en permettre la découverte, grâce aux processus qui sont décrits dans cette thèse : $pp \to W_(R) \to eN_(e) \to eejj$ et $pp \to Z' \to N_(e)N_(e) \to eejjjj$. Ceux-ci conduisent non seulement à des jets hadroniques mais aussi à des électrons dans l'état final. L'énergie et la position de ces derniers sont reconstruites dans un calorimètre électromagnétique à argon liquide. Avant d'y interagir, les électrons produits au point de collision rencontrent une certaine quantité de matière inerte, où il perdent une partie de leur énergie. Afin de compenser cet effet et ainsi maintenir la résolution en énergie à un niveau acceptable, on installe un pré-échantillonneur sur la face interne du calorimètre électromagnétique. Cette thèse en présente les principales performances.
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Rouffineau, Gilles. "Entre jeu et récit, les conditions de l'expérience esthétique dans les éditions numériques off-line." Phd thesis, Université Panthéon-Sorbonne - Paris I, 2006. http://tel.archives-ouvertes.fr/tel-00627130.
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Depuis 1990, l'édition de CDroms d'auteur a marqué l'édition numérique. Ce travail vise à interroger les conditions de réception esthétique de ces formes éditoriales. L'étude critique de cinq titres est précédée d'un panorama de ces publications. Deux principes sont privilégiés : le jeu des actions et la construction narrative, car ils en déterminent les modes de réception. Perçus comme antagonistes malgré leurs similitudes, le jeu et le récit sont l'objet d'une réflexion théorique. Celle-ci distingue jeu-game et jeu-play et insiste sur l'importance d'un niveau racontable, précédant le récit verbal. Ces distinctions permettent une articulation plus riche que l'opposition temporelle initiale entre jeu et récit qui semblait tout d'abord caractériser la réception esthétique de ces éditions. De nouvelles critiques de CDroms s'appuient sur ces distinctions, elles insistent sur l'invitation préalable induite par l'objet, la conduite des expériences engagées et les conditions de leur clôture.
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Ferrari, Christophe. "Mise en évidence de composés organiques volatils provenant de la combustion d'un biocarburant, le diester : mesure des constantes de photodégradation de composés spécifiques émis." Université Joseph Fourier (Grenoble ; 1971-2015), 1995. http://www.theses.fr/1995GRE10195.
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La deterioration de la qualite de l'air est attribuee a un accroissement des emissions des sources mobiles. L'utilisation de nouveaux carburants d'origine vegetale est testee sur les flottes de transports en commun afin de reduire ces emissions polluantes. Une comparaison des emissions entre d'une part le gazole et d'autre part un melange compose de gazole et d'esters methyliques provenant de l'huile de colza est realisee. Cette comparaison a porte sur les composes organiques volatils types alcanes, alcenes, alcynes, aromatiques, sur les aldehydes ainsi que sur les hydrocarbures aromatiques polycycliques et les particules. A cette occasion, il a ete mis en evidence des molecules specifiquement emises par ce biocarburant. Ces dernieres sont des esters methyliques a courtes chaines (de trois a six atomes de carbone). Ces composes ont fait l'objet d'etudes sur leurs interactions avec le radical hydroxyle. Leurs temps de vie dans la troposphere ont ete determines
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Mariani, Valerio. "Validazione di metodologie di simulazione dell’impatto a parete di spray di combustibile iniettati ad alta pressione." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018.
Find full textAbstract:
Il presente lavoro di tesi, svolto in collaborazione con una azienda del settore automotive, tratta lo studio di impatto di sprays combustibili su pareti calde adottando un approccio numerico CFD-3D. Viene effettuata la validazione di alcuni tra i più utilizzati modelli di interazione goccia-parete implementati nel software commerciale STAR-CD. Lo scopo principale del lavoro svolto è quello di indirizzare il lettore verso una corretta implementazione della simulazione di uno spray impattante, comprendere pregi e limiti dei modelli applicati e fornire dei criteri per effettuare la scelta tra uno di essi in funzione di condizioni di interesse motoristico.