Academic literature on the topic 'Interference reflection microscopy (IRM)'

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Journal articles on the topic "Interference reflection microscopy (IRM)"

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Verschueren, H. "Interference reflection microscopy in cell biology: methodology and applications." Journal of Cell Science 75, no. 1 (1985): 279–301. http://dx.doi.org/10.1242/jcs.75.1.279.

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Since its introduction into cell biology by Curtis in 1964, interference reflection microscopy (IRM) has been used by an increasing number of researchers to study cell-substrate interactions in living cells in culture. With the use of antiflex objectives, high-contrast IRM images can now be readily obtained. From the different theories on image formation in IRM that have been put forward, it can be seen that a zero-order interference pattern is generated at high illuminating numerical aperture. This yields information on the closeness of contact between cell and substrate, with only minor pert
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Valavanis, Dimitrios, Paolo Ciocci, Gabriel N. Meloni, et al. "Hybrid scanning electrochemical cell microscopy-interference reflection microscopy (SECCM-IRM): tracking phase formation on surfaces in small volumes." Faraday Discussions 233 (2022): 122–48. http://dx.doi.org/10.1039/d1fd00063b.

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Valavanis, Dimitrios, Paolo Ciocci, Gabriel N. Meloni, et al. "Hybrid scanning electrochemical cell microscopy-interference reflection microscopy (SECCM-IRM): tracking phase formation on surfaces in small volumes." Faraday Discussions 233 (2022): 122–48. http://dx.doi.org/10.1039/d1fd00063b.

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Zand, M. S., and G. Albrecht-Buehler. "Long-term observation of cultured cells by interference-reflection microscopy: near infrared illumination and Y-contrast image processing." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 70–71. http://dx.doi.org/10.1017/s0424820100102432.

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Analysis of dynamic changes in cell-substratum adhesion patterns during cell locomotion requires continuous, extended observation of single living cells. To date, interference-reflection microscopy (IRM) is the only method available to visualize cell -substratum adhesions in vitro. This method uses 1% of the incident illumination to produce an IRM image, and so far requires use of a high intensity visible light source (400 - 800 nm). However, light of this intensity and spectral range induces marked changes in fibroblast behavior, including cessation of locomotion. Therefore, we developed a me
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Lai, Quintin J., Stuart L. Cooper, and Ralph M. Albrecht. "Thrombus formation on artificial surfaces: Correlative microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 840–41. http://dx.doi.org/10.1017/s042482010016176x.

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Thrombus formation and embolization are significant problems for blood-contacting biomedical devices. Two major components of thrombi are blood platelets and the plasma protein, fibrinogen. Previous studies have examined interactions of platelets with polymer surfaces, fibrinogen with platelets, and platelets in suspension with spreading platelets attached to surfaces. Correlative microscopic techniques permit light microscopic observations of labeled living platelets, under static or flow conditions, followed by the observation of identical platelets by electron microscopy. Videoenhanced, dif
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Todd, I., J. S. Mellor, and D. Gingell. "Mapping cell-glass contacts of Dictyostelium amoebae by total internal reflection aqueous fluorescence overcomes a basic ambiguity of interference reflection microscopy." Journal of Cell Science 89, no. 1 (1988): 107–14. http://dx.doi.org/10.1242/jcs.89.1.107.

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The widespread ability of eukaryotic cells to produce thin cytoplasmic sheets or lamellae 100–200 nm thick can give rise to uncertainties in the interpretation of interference reflection microscopy (IRM) images when cell-substratum topography is the key interest. If allowed to spread upon a poly-L-lysine-coated surface, Dictyostelium discoideum amoebae typically form ultrathin lamellae of approximately equal to 100 nm thickness by cytoplasmic retraction. Whereas the cell body is grey, the lamellae appear very dark under IRM optics. These dark areas could be misinterpreted as stemming from a cl
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Richter, Ekkehard, Hermine Hitzler, Heiko Zimmermann, Rolf Hagedorn, and G�nter Fuhr. "Trace formation during locomotion of L929 mouse fibroblasts continuously recorded by interference reflection microscopy (IRM)." Cell Motility and the Cytoskeleton 47, no. 1 (2000): 38–47. http://dx.doi.org/10.1002/1097-0169(200009)47:1<38::aid-cm4>3.0.co;2-w.

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Singer, I. I., D. M. Kazazis, and S. Scott. "Scanning electron microscopy of focal contacts on the substratum attachment surface of fibroblasts adherent to fibronectin." Journal of Cell Science 93, no. 1 (1989): 147–54. http://dx.doi.org/10.1242/jcs.93.1.147.

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We have examined the cell-to-substratum attachment surface of hamster fibroblasts with scanning EM, and describe the surface ultrastructure of focal contacts and microspikes during cellular attachment and spreading on fibronectin. Nil 8 fibroblasts were seeded onto fibronectin-coated glass coverslips in serum-free medium, fixed, and the fibroblast-fibronectin monolayer was separated from the glass and inverted for scanning electron microscopic (EM) analysis. Focal contact development was detected by interference reflection microscopy and correlated with the immunofluorescence microscopic distr
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Paddock, S. W. "Tandem scanning reflected-light microscopy of cell-substratum adhesions and stress fibres in Swiss 3T3 cells." Journal of Cell Science 93, no. 1 (1989): 143–46. http://dx.doi.org/10.1242/jcs.93.1.143.

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This paper describes two applications of the tandem scanning reflected-light microscope (TSM) for the observation of the structure of individual cells growing in tissue culture. First, the TSM is used as an alternative to interference reflection microscopy (IRM) or total internal reflection aqueous fluorescence microscopy (TIRAF) to observe cell-substratum adhesions in unstained living cells growing on a glass coverslip. Second, the TSM is used to produce improved images of cellular structures in 3T3 cells stained with various protein dyes including Napthol Blue Black (NBB) and Coomassie Brill
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Izzard, C. S. "Optical studies on the development of the focal contact." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 120–21. http://dx.doi.org/10.1017/s0424820100102687.

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The focal contact is a localized region of relatively strong adhesion formed between cultured fibroblasts and planar substrates. The contact can be visualized and its formation followed in the live cell by the use of high illuminating-numerical-aperture interference reflection microscopy (IRM). The focal contact is the site into which stress fibers, or bundles of microfilaments, insert at the plasma membrane via a patch of amorphous material, the adhesion plaque. Through the use of immunochemical staining, a number of proteins have been shown to be concentrated at the focal contact/adhesion pl
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Dissertations / Theses on the topic "Interference reflection microscopy (IRM)"

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Ullberg, Nathan. "Visibility and charge density imaging of 2-dimensional semiconductors and devices studied using optical microscopy techniques IRM and BALM." Electronic Thesis or Diss., université Paris-Saclay, 2023. http://www.theses.fr/2023UPAST219.

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La microscopie optique a joué un rôle déterminant dans la recherche sur les matériaux bidimensionnels (2D). En particulier, les phénomènes d'interférences dans des couches minces ont été exploités pour améliorer le contraste et la résolution verticale lors de l'observation des matériaux 2D et ce jusqu'à l'échelle sub-nanométrique, souvent par l'intermédiaire de résonateurs Fabry-Pérot (FP). Dans cette thèse, la microscopie IRM (interference reflection microscopy) et la microscopie BALM (backside absorbing layer microscopy), qui abritent tous deux des effets FP, sont développées et utilisées po
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Simmert, Steve, and Erik Schäffer. "Interference reflection microscopy to visualize sub-diffraction limited objects in 3D." Diffusion fundamentals 20 (2013) 75, S. 1, 2013. https://ul.qucosa.de/id/qucosa%3A13662.

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Simmert, Steve, and Erik Schäffer. "Interference reflection microscopy to visualize sub-diffraction limited objects in 3D." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-183633.

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Simmert, Steve [Verfasser], and Erik [Akademischer Betreuer] Schäffer. "Optical tweezers combined with interference reflection microscopy for quantitative trapping and 3D imaging / Steve Simmert ; Betreuer: Erik Schäffer." Tübingen : Universitätsbibliothek Tübingen, 2018. http://d-nb.info/1199268771/34.

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Book chapters on the topic "Interference reflection microscopy (IRM)"

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Kihm, Kenneth D. "Reflection Interference Contrast Microscopy (RICM)." In Near-Field Characterization of Micro/Nano-Scaled Fluid Flows. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-20426-5_6.

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Rädler, J., and E. Sackmann. "Vesicle-Substrate Interaction Studied by Reflection Interference Contrast Microscopy." In Springer Proceedings in Physics. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84763-9_30.

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Curtis, A. S. G. "Interference Reflection Microscopy and Related Microscopies and Cell Adhesion." In Studying Cell Adhesion. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-03008-0_13.

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Abdelrahman, Ahmed, Ana-Sunčana Smith, and Kheya Sengupta. "Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy." In The Immune Synapse. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3135-5_8.

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Schürch, S., F. Green, M. Schoel, and P. Gehr. "Adhesion of Pulmonary Macrophages to Langmuir-Blodgett Films, Investigated by Interference Reflection Microscopy." In Springer Series in Biophysics. Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73925-5_44.

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Fletcher, Madilyn. "The Application of Interference Reflection Microscopy to the Study of Bacterial Adhesion to Solid Surfaces." In Biodeterioration 7. Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-1363-9_4.

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"IRM (interference-reflection microscopy)." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_8753.

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Ploem, J. S., F. A. Prins, and I. Cornelese-Ten Velde. "Reflection-contrast microscopy." In Light Microscopy in Biology. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780199636709.003.0007.

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Abstract Fauré-Fremiet (1) used reflected light in cell biology studies half a century ago. Curtis (2) and Izzard and Lochner (3) performed the first fundamental studies of interference images of living cells on glass surfaces using reflected light microscopy. Reflection images were obtained with scanning reflecting microscopy (4, 5). Reflected-light microscopy for the study of living cells (2, 3, 6, 7) has been described as interference reflection, reflection-interference contrast, surface-contrast, and surface-:reflection interference microscopy (8). Ploem (9, 10) investigated further optica
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Weber, Igor. "[2] Reflection interference contrast microscopy." In Methods in Enzymology. Elsevier, 2003. http://dx.doi.org/10.1016/s0076-6879(03)61004-9.

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Ruzin, Steven E. "Epifluorescence Microscopy." In Techniques in Light Microscopy. Oxford University PressOxford, 2024. http://dx.doi.org/10.1093/oso/9780198885832.003.0008.

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Abstract This chapter on fluorescence microscopy provides the theoretical background for all microscope techniques that use fluorescence for imaging samples. It covers the theory of fluorescence and its implementation in microscopy. Here interference filters and dichroic mirrors are described in the context of the ubiquitous epifluorescence microscope, and all fluorescence microscopes and techniques that follow The concepts of photobleaching, the Stokes shift, and fluorescence quantum yield are also discussed. Included is a description of the optical components required for epifluorescence inc
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Conference papers on the topic "Interference reflection microscopy (IRM)"

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Opas, Michal, and Michal Opas. "Biomedical Applications Of Interference Reflection Microscopy." In Interferometry '89, edited by Zbigniew Jaroszewicz, Maksymilian Pluta, Zbigniew Jaroszewicz, and Maksymilian Pluta. SPIE, 1990. http://dx.doi.org/10.1117/12.961294.

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Davies, Heather S., Natalia S. Baranova, Nouha El Amri, et al. "Blood cell - vessel wall interactions probed by reflection interference contrast microscopy." In Advances in Microscopic Imaging, edited by Francesco S. Pavone, Emmanuel Beaurepaire, and Peter T. So. SPIE, 2019. http://dx.doi.org/10.1117/12.2527058.

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Lee, Byron S., and T. C. Strand. "Scanning Interference Microscopy for Surface Characterization." In Optical Fabrication and Testing. Optica Publishing Group, 1988. http://dx.doi.org/10.1364/oft.1988.tha8.

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Recent work has introduced the concept of scanning interference microscopy which has 3-D resolution comparable to a confocal microscope (1). This is obtained by performing interference microscopy with spatially incoherent and broadband illumination. By scanning along the optical axis, one can measure the coherence function at each point in the image. This coherence function can be processed to obtain various pieces of information. The maximum of the envelope of the coherence function corresponds to the surface height. By Fourier processing of the fringes at each point, the spectral reflectivit
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Kandel, Mikhail E., Catherine Best-Popescu, and Gabriel Popescu. "Reflection gradient light interference microscopy (epi-GLIM) for label-free imaging of bulk specimens (Conference Presentation)." In Quantitative Phase Imaging IV, edited by Gabriel Popescu and YongKeun Park. SPIE, 2018. http://dx.doi.org/10.1117/12.2294032.

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Sakovina, L. V., A. V. Endutkin, and D. O. Zharkov. "INTERFEROMETRIC SCATTERING MICROSCOPY FOR INVESTIGATION SPYCAS9 ENDONUCLEASE AND GUIDE SGRNA COMPLEX." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-268.

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Using interferometric scattering microscopy (ISM), based on the principles of interference-reflection microscopy and interferometric scattering microscopy [1], it is possible to measure the molecular masses of individual molecules. In this study, we evaluated the ISM method to investigate the biochemical properties of the SpyCas9 endonuclease and SpyCas9 and guide sgRNA complex.
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Lin, J. A., and W. T. Yeh. "A Grating Interferometer For Testing The Zone Plate." In Optical Fabrication and Testing. Optica Publishing Group, 1988. http://dx.doi.org/10.1364/oft.1988.thb10.

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The zone plate is a diffraction optical element which images by interference, rather than by reflection or refraction. The applications of the zone plate include X-ray microscopy, focusing and coupling elements in opto-electronics, X-ray astronomy and etc. The focusing profile of the zone plate has been studied quite well. However, the wavefront aberration of the zone plate was less tested or evaluated by the interferometer1. We use a sinusoidal grating in the Ronchi interferometer to split the wavefront under test into three. The wavefront aberration for both amplitude and phase zone plate ma
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Lee, Dooyoung, Karen P. Fong, Lawrence F. Brass, and Daniel A. Hammer. "Dynamic Spreading of Platelets on Collagen in Microchannels." In ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2009. http://dx.doi.org/10.1115/icnmm2009-82247.

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Platelets come contact collagen exposed on the subendothelial matrix at sites of vascular injury that triggers their activation and the formation of a hemostatic plug. Glycoprotein VI and integrin α2β1 are major collagen receptors on the platelet surface. Although the spreading of platelets on the collagen is important to function, it has been difficult to study the dynamics of spreading over a relevant time scale. Here we focus on the early stages of murine platelet spreading on collagen and/or fibrinogen under both static conditions and flow and then probe their dynamics by quantitative visu
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