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Journal articles on the topic 'Interference reflection microscopy (IRM)'

Author: Grafiati

Published: 7 July 2024

Last updated: 31 July 2025

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1

Verschueren, H. "Interference reflection microscopy in cell biology: methodology and applications." Journal of Cell Science 75, no. 1 (1985): 279–301. http://dx.doi.org/10.1242/jcs.75.1.279.

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Since its introduction into cell biology by Curtis in 1964, interference reflection microscopy (IRM) has been used by an increasing number of researchers to study cell-substrate interactions in living cells in culture. With the use of antiflex objectives, high-contrast IRM images can now be readily obtained. From the different theories on image formation in IRM that have been put forward, it can be seen that a zero-order interference pattern is generated at high illuminating numerical aperture. This yields information on the closeness of contact between cell and substrate, with only minor pert

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2

Valavanis, Dimitrios, Paolo Ciocci, Gabriel N. Meloni, et al. "Hybrid scanning electrochemical cell microscopy-interference reflection microscopy (SECCM-IRM): tracking phase formation on surfaces in small volumes." Faraday Discussions 233 (2022): 122–48. http://dx.doi.org/10.1039/d1fd00063b.

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3

Valavanis, Dimitrios, Paolo Ciocci, Gabriel N. Meloni, et al. "Hybrid scanning electrochemical cell microscopy-interference reflection microscopy (SECCM-IRM): tracking phase formation on surfaces in small volumes." Faraday Discussions 233 (2022): 122–48. http://dx.doi.org/10.1039/d1fd00063b.

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4

Zand, M. S., and G. Albrecht-Buehler. "Long-term observation of cultured cells by interference-reflection microscopy: near infrared illumination and Y-contrast image processing." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 70–71. http://dx.doi.org/10.1017/s0424820100102432.

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Analysis of dynamic changes in cell-substratum adhesion patterns during cell locomotion requires continuous, extended observation of single living cells. To date, interference-reflection microscopy (IRM) is the only method available to visualize cell -substratum adhesions in vitro. This method uses 1% of the incident illumination to produce an IRM image, and so far requires use of a high intensity visible light source (400 - 800 nm). However, light of this intensity and spectral range induces marked changes in fibroblast behavior, including cessation of locomotion. Therefore, we developed a me

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Lai, Quintin J., Stuart L. Cooper, and Ralph M. Albrecht. "Thrombus formation on artificial surfaces: Correlative microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 840–41. http://dx.doi.org/10.1017/s042482010016176x.

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Thrombus formation and embolization are significant problems for blood-contacting biomedical devices. Two major components of thrombi are blood platelets and the plasma protein, fibrinogen. Previous studies have examined interactions of platelets with polymer surfaces, fibrinogen with platelets, and platelets in suspension with spreading platelets attached to surfaces. Correlative microscopic techniques permit light microscopic observations of labeled living platelets, under static or flow conditions, followed by the observation of identical platelets by electron microscopy. Videoenhanced, dif

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6

Todd, I., J. S. Mellor, and D. Gingell. "Mapping cell-glass contacts of Dictyostelium amoebae by total internal reflection aqueous fluorescence overcomes a basic ambiguity of interference reflection microscopy." Journal of Cell Science 89, no. 1 (1988): 107–14. http://dx.doi.org/10.1242/jcs.89.1.107.

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The widespread ability of eukaryotic cells to produce thin cytoplasmic sheets or lamellae 100–200 nm thick can give rise to uncertainties in the interpretation of interference reflection microscopy (IRM) images when cell-substratum topography is the key interest. If allowed to spread upon a poly-L-lysine-coated surface, Dictyostelium discoideum amoebae typically form ultrathin lamellae of approximately equal to 100 nm thickness by cytoplasmic retraction. Whereas the cell body is grey, the lamellae appear very dark under IRM optics. These dark areas could be misinterpreted as stemming from a cl

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7

Richter, Ekkehard, Hermine Hitzler, Heiko Zimmermann, Rolf Hagedorn, and G�nter Fuhr. "Trace formation during locomotion of L929 mouse fibroblasts continuously recorded by interference reflection microscopy (IRM)." Cell Motility and the Cytoskeleton 47, no. 1 (2000): 38–47. http://dx.doi.org/10.1002/1097-0169(200009)47:1<38::aid-cm4>3.0.co;2-w.

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8

Singer, I. I., D. M. Kazazis, and S. Scott. "Scanning electron microscopy of focal contacts on the substratum attachment surface of fibroblasts adherent to fibronectin." Journal of Cell Science 93, no. 1 (1989): 147–54. http://dx.doi.org/10.1242/jcs.93.1.147.

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We have examined the cell-to-substratum attachment surface of hamster fibroblasts with scanning EM, and describe the surface ultrastructure of focal contacts and microspikes during cellular attachment and spreading on fibronectin. Nil 8 fibroblasts were seeded onto fibronectin-coated glass coverslips in serum-free medium, fixed, and the fibroblast-fibronectin monolayer was separated from the glass and inverted for scanning electron microscopic (EM) analysis. Focal contact development was detected by interference reflection microscopy and correlated with the immunofluorescence microscopic distr

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9

Paddock, S. W. "Tandem scanning reflected-light microscopy of cell-substratum adhesions and stress fibres in Swiss 3T3 cells." Journal of Cell Science 93, no. 1 (1989): 143–46. http://dx.doi.org/10.1242/jcs.93.1.143.

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This paper describes two applications of the tandem scanning reflected-light microscope (TSM) for the observation of the structure of individual cells growing in tissue culture. First, the TSM is used as an alternative to interference reflection microscopy (IRM) or total internal reflection aqueous fluorescence microscopy (TIRAF) to observe cell-substratum adhesions in unstained living cells growing on a glass coverslip. Second, the TSM is used to produce improved images of cellular structures in 3T3 cells stained with various protein dyes including Napthol Blue Black (NBB) and Coomassie Brill

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10

Izzard, C. S. "Optical studies on the development of the focal contact." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 120–21. http://dx.doi.org/10.1017/s0424820100102687.

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The focal contact is a localized region of relatively strong adhesion formed between cultured fibroblasts and planar substrates. The contact can be visualized and its formation followed in the live cell by the use of high illuminating-numerical-aperture interference reflection microscopy (IRM). The focal contact is the site into which stress fibers, or bundles of microfilaments, insert at the plasma membrane via a patch of amorphous material, the adhesion plaque. Through the use of immunochemical staining, a number of proteins have been shown to be concentrated at the focal contact/adhesion pl

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11

Yuruker, B., and V. Niggli. "Alpha-actinin and vinculin in human neutrophils: reorganization during adhesion and relation to the actin network." Journal of Cell Science 101, no. 2 (1992): 403–14. http://dx.doi.org/10.1242/jcs.101.2.403.

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We have studied the reorganization of vinculin and alpha-actinin during the process of adhesion in human neutrophils using immunofluorescence microscopy and interference reflection microscopy (IRM). Neutrophils in contact with uncoated glass formed black IRM areas in the cell periphery, indicative of very close contact with the substratum. Eight to twelve minutes after addition of cells to glass, vinculin was found to become concentrated in small patches at the cell periphery, partially colocalizing with the black IRM areas and with small F-actin-containing adherent protrusions. In contrast, v

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12

Owens, N. F., D. Gingell, and A. Trommler. "Cell adhesion to hydroxyl groups of a monolayer film." Journal of Cell Science 91, no. 2 (1988): 269–79. http://dx.doi.org/10.1242/jcs.91.2.269.

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We have studied cells on chemically defined monomolecular films of the long-chain alcohol docosanol. Langmuir-Blodgett films of the alcohol were deposited on glass coverslips, previously made hydrophobic with octadecyl groups. This gives films in which the alcohol headgroups face outwards to the water. Molecular orientation and film integrity were shown by a fluorescence adsorption test. Cell contacts on the films were observed in media without proteins by interference reflection microscopy (IRM) and the mechanics of detachment were examined by hydrodynamic shearing in a flow chamber. Cell con

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13

Keating, Blane, Ian McPherson, Dimitrious Valavanis, Aaron-Jerome Agyei, and Patrick Unwin. "Seccm-IRM: A New Tool for Quantitative in Situ Studies of Crystal Growth." ECS Meeting Abstracts MA2022-01, no. 24 (2022): 2498. http://dx.doi.org/10.1149/ma2022-01242498mtgabs.

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Scanning electrochemical cell microscopy (SECCM) is a versatile scanning probe imaging technique that allows for simultaneous elucidation of structure activity relationships at the nanoscale in defined electrolyte volumes and provides high resolution (nm length scale) information on the topography of surfaces and interfaces1. Since its inception in 2010 SECCM has improved understanding of model systems such as graphene, graphite, carbon nanotubes, nanoparticles and conductive diamond and provided electrochemists with a tool for true single entity measurements. Electrodeposition of microscale t

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14

Bailey, J., and D. Gingell. "Contacts of chick fibroblasts on glass: results and limitations of quantitative interferometry." Journal of Cell Science 90, no. 2 (1988): 215–24. http://dx.doi.org/10.1242/jcs.90.2.215.

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We have examined the contacts made by explanted chick heart and limb bud fibroblasts after 24–48 h on glass, using quantitative interference reflection microscopy (IRM). Contacts beneath very thin cytoplasmic lamellae were avoided because the images of such contacts depend on the thickness of the lamellae. Plaque-like focal contacts, distinguished on the basis of shape and low irradiance (darkness), are intimate adhesions to the substratum. These images can be interpreted if it is assumed that microfilaments associated with the lower membrane increase the local cytoplasmic refractive index. Th

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15

DePasquale, J. A., and C. S. Izzard. "Evidence for an actin-containing cytoplasmic precursor of the focal contact and the timing of incorporation of vinculin at the focal contact." Journal of Cell Biology 105, no. 6 (1987): 2803–9. http://dx.doi.org/10.1083/jcb.105.6.2803.

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The distribution of F-actin and vinculin in chicken embryo fibroblasts has been examined by nitrobenzoxadiazol (NBD)-phallacidin and indirect immunofluorescent staining, respectively, and related to the process of focal contact formation by recording the motility of the cell with differential interference contrast (DIC) or interference reflection microscopy (IRM) before fixation for staining. Linear cytoplasmic precursors of the focal contact, present within unattached lamellipodia, stained intensely with NBD-phallacidin. Without exception new focal contacts, 8 s and older at fixation, were as

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16

Woods, A., and J. R. Couchman. "Protein kinase C involvement in focal adhesion formation." Journal of Cell Science 101, no. 2 (1992): 277–90. http://dx.doi.org/10.1242/jcs.101.2.277.

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Matrix molecules such as fibronectin can promote cell attachment, spreading and focal adhesion formation. Although some interactions of fibronectin with cell surface receptors have now been identified, the consequent activation of intracellular messenger systems by cell/matrix interactions have still to be elucidated. We show here that the kinase inhibitors H7 and HA1004 reduce focal adhesion and stress fiber formation in response to fibronectin in a dose-dependent manner, and that activators of protein kinase C can promote their formation under conditions where they do not normally form. Fibr

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17

Zheng, J., R. E. Buxbaum, and S. R. Heidemann. "Measurements of growth cone adhesion to culture surfaces by micromanipulation." Journal of Cell Biology 127, no. 6 (1994): 2049–60. http://dx.doi.org/10.1083/jcb.127.6.2049.

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Neurons were grown on plastic surfaces that were untreated, or treated with polylysine, laminin, or L1 and their growth cones were detached from their culture surface by applying known forces with calibrated glass needles. This detachment force was taken as a measure of the force of adhesion of the growth cone. We find that on all surfaces, lamellipodial growth cones require significantly greater detachment force than filopodial growth cones, but this differences is, in general, due to the greater area of lamellipodial growth cones compared to filopodial growth cones. That is, the stress (forc

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18

Martín-Yerga, Daniel, Xiangdong Xu, Dimitrios Valavanis, Geoff West, Marc Walker, and Patrick Unwin. "High-Throughput Combinatorial Analysis of the Spatiotemporal Dynamics of Nanoscale Lithium Metal Plating." ACS Nano 18, no. 34 (2024): 23032–46. https://doi.org/10.1021/acsnano.4c05001.

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The development of Li metal batteries requires a detailed understanding of complex nucleation and growth processes during electrodeposition. <em>In situ</em> techniques offer a framework to study these phenomena by visualizing structural dynamics that can inform the design of uniform plating morphologies. Herein, we combine scanning electrochemical cell microscopy (SECCM) with <em>in situ</em> interference reflection microscopy (IRM) for a comprehensive investigation of Li nucleation and growth on lithiophilic thin-film gold electrodes. This multimicroscopy approach enables

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19

Park, Jeesun, David R. Fooksman, Amitabha Mazumder, and Michael L. Dustin. "Multiple Myeloma Cells Adhere to Netrin-1 Via Heparin-Sulphate Moieties." Blood 120, no. 21 (2012): 3952. http://dx.doi.org/10.1182/blood.v120.21.3952.3952.

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Abstract Abstract 3952 A major obstacle to the treatment of Multiple Myeloma (MM) is the localization of myeloma cells to the bone marrowstroma, enabling drug resistance. The exact mechanisms of adhesion of myeloma cells to the bone marrow are not known, but adhesion molecules and chemokine signals, in particular vascular cell adhesion protein 1 (VCAM-1) and C-X-C chemokine 12 (CXCL12) which control bone marrow tropism, are thought to be the main players. Netrin-1, which acts as an axonal guidance cue, plays a role in leukocyte migration in lymph nodes and in atherosclerotic lesions, but has n

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20

Ramsay, Alan G., Rachel Evans, Lena Svensson, Shahryar Kiaii, Nancy Hogg, and John G. Gribben. "Defective LFA-1 Mediated T Cell Motility In Chronic Lymphocytic Leukemia Is Mediated by Defects In the Rho GTPase Signaling Pathway." Blood 116, no. 21 (2010): 914. http://dx.doi.org/10.1182/blood.v116.21.914.914.

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Abstract Abstract 914 T lymphocytes have an essential role in adaptive immunity and rely on tightly regulated signaling through integrin lymphocyte function-associated antigen (LFA)-1 to migrate into lymph nodes and interact with antigen-presenting cells. Malignant cells modify their immune microenvironment to prevent effective host anti-tumor responses, promote tumor progression, and suppress the therapeutic benefit of immunotherapy treatments. Here we assessed LFA-1-mediated cell migration of highly purified T cells from treatment naïve chronic lymphocytic leukemia (CLL) patients compared t

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21

Kojima, Seiji. "Interference Fringes in Reflection Acoustic Microscopy." Japanese Journal of Applied Physics 26, S1 (1987): 233. http://dx.doi.org/10.7567/jjaps.26s1.233.

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22

Gingell, D., O. S. Heavens, and J. S. Mellor. "General electromagnetic theory of total internal reflection fluorescence: the quantitative basis for mapping cell-substratum topography." Journal of Cell Science 87, no. 5 (1987): 677–93. http://dx.doi.org/10.1242/jcs.87.5.677.

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Total internal reflection fluorescence (TIRF) has recently been used to look at the contacts made between cells and a glass surface on which they are spread. Our method utilizes the fluorescence of a water-soluble dye that acts as an extracellular aqueous volume marker. Fluorescence is stimulated by the short-range electric field near the glass surface that exists under conditions of total internal reflection. Since fluorescence is normally generated beneath a spread cell and not beyond it, the fluorescence of the image is related to the size of the cell-glass water gap. The images obtained ar

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23

Velas, Lukas, Philipp Zelger, Alexander Jesacher, and Gerhard J. Schütz. "Correlating Interference Reflection Microscopy with 3D Superresolution Fluorescence Microscopy." Biophysical Journal 120, no. 3 (2021): 180a. http://dx.doi.org/10.1016/j.bpj.2020.11.1254.

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24

Chmelik, Christian, and Jörg Kärger. "Unprecedented Wealth of Information on Guest Dynamics in Nanoporous Materials from Transient Concentration Profiles." Defect and Diffusion Forum 309-310 (March 2011): 177–94. http://dx.doi.org/10.4028/www.scientific.net/ddf.309-310.177.

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The application of interference microscopy (IFM) and infrared microscopy (IRM) to monitor the evolution of the concentration of guest molecules in nanoporous host materials opens a new field of diffusion research in condensed matter. It combines the methodical virtues of the profiling methods of solid-state diffusion studies with the benefit of the mobility enhancement in fluids. We are going to illustrate the rich options of diffusion studies provided by this novel experimental approach.

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25

Kim, Kipom, and Omar A. Saleh. "Stabilizing method for reflection interference contrast microscopy." Applied Optics 47, no. 12 (2008): 2070. http://dx.doi.org/10.1364/ao.47.002070.

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26

Contreras-Naranjo, Jose C., James A. Silas, and Victor M. Ugaz. "Reflection interference contrast microscopy of arbitrary convex surfaces." Applied Optics 49, no. 19 (2010): 3701. http://dx.doi.org/10.1364/ao.49.003701.

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27

YOSHIKAWA, Hiroshi Y., and Takahisa MATSUZAKI. "Reflection Interference Microscopy~Noninvasive Nano-Imaging of Soft Interfaces~." Seibutsu Butsuri 57, no. 6 (2017): 318–22. http://dx.doi.org/10.2142/biophys.57.318.

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28

Hillner, P. E., M. Radmacher, and P. K. Hansma. "Combined atomic force and scanning reflection interference contrast microscopy." Scanning 17, no. 3 (2006): 144–47. http://dx.doi.org/10.1002/sca.4950170304.

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29

Pahl, Tobias, Johannes Breidenbach, and Peter Lehmann. "Quasi-analytical and rigorous modeling of interference microscopy." EPJ Web of Conferences 266 (2022): 10013. http://dx.doi.org/10.1051/epjconf/202226610013.

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We present an extended vectorial Kirchhoff model of coherence scanning interferometry including several vector rotations occurring in the imagining and scattering process as well as polarization dependent reflection coefficients. For validation simulated results are compared to those of the conventional scalar Kirchhoff model and a rigorous finite element modeling.

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30

ICHIKAWA, M., S. MARUNO, S. FUJITA, H. WATANABE, and Y. KUSUMI. "MICROPROBE RHEED/STM COMBINED MICROSCOPY." Surface Review and Letters 04, no. 03 (1997): 535–42. http://dx.doi.org/10.1142/s0218625x97000511.

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We have developed microprobe reflection high energy electron diffraction combined with scanning tunneling microscope and molecular beam epitaxy equipment. This combination makes it possible to study and control surface processes in the magnification range from several hundred micrometers to the atomic scale. An electron biprism is also attached to the incident electron beam path, which produces a new kind of scanning electron microscopy called scanning interference electron microscopy. The two coherently divided electron beams created by the biprism produce electron interference fringes. The e

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31

Saper, Gadiel, and Henry Hess. "Kinesin-propelled label-free microtubules imaged with interference reflection microscopy." New Journal of Physics 22, no. 9 (2020): 095002. http://dx.doi.org/10.1088/1367-2630/abb47b.

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32

Monzel, Cornelia, Susanne F. Fenz, Rudolf Merkel, and Kheya Sengupta. "Probing Biomembrane Dynamics by Dual-Wavelength Reflection Interference Contrast Microscopy." ChemPhysChem 10, no. 16 (2009): 2828–38. http://dx.doi.org/10.1002/cphc.200900645.

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33

Keith, C. H., and R. Witek. "Enhancement of Reflection-Enhanced Backscatter Confocal Microscopy." Microscopy and Microanalysis 7, S2 (2001): 1014–15. http://dx.doi.org/10.1017/s1431927600031147.

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Tremendous advances have been made in the last twenty years in extending the usefulness of the light microscope in visualizing living biological specimens, and it is now possible to visualize most thin tissues in an unstained state at – or near – the limit of optical resolution. in general, however, these techniques have been used successfully on biological specimens in the transmitted light mode; unstained biological specimens generally do not have sufficient albedo or sufficient difference in refractive index from their surroundings to be efficiently visualized in epi-illumination. A number

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34

Stott, D., and C. C. Wylie. "Invasive behaviour of mouse primordial germ cells in vitro." Journal of Cell Science 86, no. 1 (1986): 133–44. http://dx.doi.org/10.1242/jcs.86.1.133.

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We have isolated migrating primordial germ cells (PGCs) from 10.5-day mouse embryos and studied their behaviour when cultured on a mouse embryo fibroblast (STO) cell line. Living and fixed PGCs were identified by fluorescent labelling with a monoclonal antibody specific for PGCs in the culture system used. The behaviour of the cells was studied using interference reflexion microscopy (IRM) and time-lapse video cinematography. The IRM pattern displayed by PGCs is typical of highly motile cell types, the cells lack focal contacts and possess large areas of close contacts indicative of weak membr

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35

Dubois, Arnaud. "Effects of phase change on reflection in phase-measuring interference microscopy." Applied Optics 43, no. 7 (2004): 1503. http://dx.doi.org/10.1364/ao.43.001503.

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36

Weber, Igor, and Richard Albrecht. "Image processing for combined bright-field and reflection interference contrast video microscopy." Computer Methods and Programs in Biomedicine 53, no. 2 (1997): 113–18. http://dx.doi.org/10.1016/s0169-2607(97)01810-5.

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37

CHIU, L. ‐D, L. SU, S. REICHELT, and W. B. AMOS. "Use of a white light supercontinuum laser for confocal interference‐reflection microscopy." Journal of Microscopy 246, no. 2 (2012): 153–59. http://dx.doi.org/10.1111/j.1365-2818.2012.03603.x.

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38

HOLT, M. R., Y. CALLE, D. H. SUTTON, D. R. CRITCHLEY, G. E. JONES, and G. A. DUNN. "Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy." Journal of Microscopy 232, no. 1 (2008): 73–81. http://dx.doi.org/10.1111/j.1365-2818.2008.02069.x.

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39

Limozin, Laurent, and Kheya Sengupta. "Quantitative Reflection Interference Contrast Microscopy (RICM) in Soft Matter and Cell Adhesion." ChemPhysChem 10, no. 16 (2009): 2752–68. http://dx.doi.org/10.1002/cphc.200900601.

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40

Kärger, Jörg, and Rustem Valiullin. "Transport-Optimized Nanoporous Materials for Mass Separation and Conversion as Designed by Microscopic Diffusion Measurement." Diffusion Foundations 19 (November 2018): 96–124. http://dx.doi.org/10.4028/www.scientific.net/df.19.96.

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Nanoporous materials find widespread application in material upgrading by separation (“molecular sieving”) and catalytic conversion. Mass transfer in these materials is a key phenomenon deciding about their technological performance. This chapter deals with the application of measurement techniques which are able to follow the diffusive fluxes of the guest molecules in such materials over “microscopic” distances, including the pulsed field gradient (PFG) technique of Nuclear Magnetic Resonance (NMR) and the techniques of microimaging by interference microscopy (IFM) and by IR microscopy (IRM).

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Huerre, A., M. C. Jullien, O. Theodoly, and M. P. Valignat. "Absolute 3D reconstruction of thin films topography in microfluidic channels by interference reflection microscopy." Lab on a Chip 16, no. 5 (2016): 911–16. http://dx.doi.org/10.1039/c5lc01417d.

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The topography of thin films in microfluidic channels can be reconstructed at the nanometric scale from interference microscopy imaging by modelling the multiple reflections at the upper and the lower surfaces of the microchannel.

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42

Bohannon, Kevin P., Ronald W. Holz, and Daniel Axelrod. "Refractive Index Imaging of Cells with Variable-Angle Near-Total Internal Reflection (TIR) Microscopy." Microscopy and Microanalysis 23, no. 5 (2017): 978–88. http://dx.doi.org/10.1017/s1431927617012570.

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AbstractThe refractive index in the interior of single cells affects the evanescent field depth in quantitative studies using total internal reflection (TIR) fluorescence, but often that index is not well known. We here present method to measure and spatially map the absolute index of refraction in a microscopic sample, by imaging a collimated light beam reflected from the substrate/buffer/cell interference at variable angles of incidence. Above the TIR critical angle (which is a strong function of refractive index), the reflection is 100%, but in the immediate sub-critical angle zone, the ref

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43

Terborg, Roland A., Josselin Pello, Ilaria Mannelli, Juan P. Torres, and Valerio Pruneri. "Ultrasensitive interferometric on-chip microscopy of transparent objects." Science Advances 2, no. 6 (2016): e1600077. http://dx.doi.org/10.1126/sciadv.1600077.

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Light microscopes can detect objects through several physical processes, such as scattering, absorption, and reflection. In transparent objects, these mechanisms are often too weak, and interference effects are more suitable to observe the tiny refractive index variations that produce phase shifts. We propose an on-chip microscope design that exploits birefringence in an unconventional geometry. It makes use of two sheared and quasi-overlapped illuminating beams experiencing relative phase shifts when going through the object, and a complementary metal-oxide-semiconductor image sensor array to

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44

Dupuis, Julia Rentz, James Needham, Emre Özkumur, et al. "Hyperspectral Fourier transform spectrometer for reflection spectroscopy and spectral self-interference fluorescence microscopy." Applied Optics 47, no. 9 (2008): 1223. http://dx.doi.org/10.1364/ao.47.001223.

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45

Fang, Ning, Vincent Chan, Kai-Tak Wan, Hai-Quan Mao, and Kam W. Leong. "Colloidal adhesion of phospholipid vesicles: high-resolution reflection interference contrast microscopy and theory." Colloids and Surfaces B: Biointerfaces 25, no. 4 (2002): 347–62. http://dx.doi.org/10.1016/s0927-7765(01)00336-8.

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46

Opas, Michał, and Vitauts I. Kalnins. "Multiple Labeling of Cellular Constituents by Combining Surface Reflection Interference and Fluorescence Microscopy." Pathobiology 53, no. 5 (1985): 241–51. http://dx.doi.org/10.1159/000163318.

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47

Streeter, H. B., and D. A. Rees. "Fibroblast adhesion to RGDS shows novel features compared with fibronectin." Journal of Cell Biology 105, no. 1 (1987): 507–15. http://dx.doi.org/10.1083/jcb.105.1.507.

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As previously shown by others, the fibroblast attachment and spreading activity of fibronectin is mimicked by a short peptide (RGDS or longer) from the cell binding domain. Normal rat kidney fibroblasts showed similar attachment kinetics on either peptide GRGDSC or bovine plasma fibronectin and binding to either substratum was inhibited by peptide alone. We now demonstrate, however, considerable differences in biological activity between peptide and fibronectin. In particular, cells developed novel adhesion structures on peptide-coated substrata. Interference reflection microscopy showed a pre

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Joshi, N. V., and H. Medina. "Multiple Beam Interference Confocal Microscopy: Tool for Morphological Investigation of a Living Spermatozoon." Microscopy and Microanalysis 6, no. 5 (2000): 471–77. http://dx.doi.org/10.1007/s100059910020.

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AbstractThe interference pattern obtained from a multiple internal reflection of a spermatozoon, sandwiched between the glass plate and the cover plate, was focused on the objective of a scanning confocal microscope. According to optical path differences, morphological details were revealed. The combined features, namely improved resolution in z axis, originating from the interference pattern and the optical sectioning of the confocal scanning system, enhanced the resolution and contrast in an impressive manner. These features permitted unprecedented images of the spermatozoon to be obtained a

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Silk, Ely. "Reflected/Transmitted Nomarsky DIC Lighting." Microscopy Today 6, no. 4 (1998): 8–10. http://dx.doi.org/10.1017/s1551929500067195.

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What could be better than reflected or transmitted Nomarski differential interference contrast? Why, combining the best features of both and for very little cost. My intended use of the technique was for Nomarski reflection DIC microscopy. It will work, of course, with other types of reflection microscopy.What this embarrassingly simple artifice accomplishes is to simultaneously add transmission capabilities to reflection observations with the result being improved viewing of delicate details, And, yes, because of the reflective front surface layer, the observer can study details on the bottom

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Lopez-Ayon, G. Monserratt, David J. Oliver, Peter H. Grutter, and Svetlana V. Komarova. "Deconvolution of Calcium Fluorescent Indicator Signal from AFM Cantilever Reflection." Microscopy and Microanalysis 18, no. 4 (2012): 808–15. http://dx.doi.org/10.1017/s1431927612000402.

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AbstractAtomic force microscopy (AFM) can be combined with fluorescence microscopy to measure the changes in intracellular calcium levels (indicated by fluorescence of Ca2+ sensitive dye fluo-4) in response to mechanical stimulation performed by AFM. Mechanical stimulation using AFM is associated with cantilever movement, which may interfere with the fluorescence signal. The motion of the AFM cantilever with respect to the sample resulted in changes of the reflection of light back to the sample and a subsequent variation in the fluorescence intensity, which was not related to changes in intrac

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