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Journal articles on the topic 'Internal transcribed spacer 1'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

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1

van der Heijden, Harold M. J. F., Wil J. M. Landman, Sophie Greve, and Ron Peek. "Genotyping ofHistomonas meleagridisisolates based on Internal Transcribed Spacer-1 sequences." Avian Pathology 35, no. 4 (2006): 330–34. http://dx.doi.org/10.1080/03079450600815499.

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2

Booy, G., J. Van der Schoot, and B. Vosman. "Heterogeneity of the internal transcribed spacer 1 (ITS1) inTulipa (Liliaceae)." Plant Systematics and Evolution 225, no. 1-4 (2000): 29–41. http://dx.doi.org/10.1007/bf00985457.

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3

Carbone, Ignazio, and Linda M. Kohn. "Ribosomal DNA Sequence Divergence within Internal Transcribed Spacer 1 of the Sclerotiniaceae." Mycologia 85, no. 3 (1993): 415. http://dx.doi.org/10.2307/3760703.

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4

Lebuhn, Michael, Stephan Bathe, Wafa Achouak, Anton Hartmann, Thierry Heulin, and Michael Schloter. "Comparative sequence analysis of the internal transcribed spacer 1 of Ochrobactrum species." Systematic and Applied Microbiology 29, no. 4 (2006): 265–75. http://dx.doi.org/10.1016/j.syapm.2005.11.003.

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5

Henry, Travis, Peter C. Iwen, and Steven H. Hinrichs. "Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2." Journal of Clinical Microbiology 38, no. 4 (2000): 1510–15. http://dx.doi.org/10.1128/jcm.38.4.1510-1515.2000.

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Aspergillus species are the most frequent cause of invasive mold infections in immunocompromised patients. Although over 180 species are found within the genus, 3 species, Aspergillus flavus, A. fumigatus, and A. terreus, account for most cases of invasive aspergillosis (IA), with A. nidulans, A. niger, and A. ustus being rare causes of IA. The ability to distinguish between the various clinically relevant Aspergillus species may have diagnostic value, as certain species are associated with higher mortality and increased virulence and vary in their resistance to antifungal therapy. A method to identify Aspergillus at the species level and differentiate it from other true pathogenic and opportunistic molds was developed using the 18S and 28S rRNA genes for primer binding sites. The contiguous internal transcribed spacer (ITS) region, ITS 1–5.8S–ITS 2, from referenced strains and clinical isolates of aspergilli and other fungi were amplified, sequenced, and compared with non-reference strain sequences in GenBank. ITS amplicons fromAspergillus species ranged in size from 565 to 613 bp. Comparison of reference strains and GenBank sequences demonstrated that both ITS 1 and ITS 2 regions were needed for accurate identification ofAspergillus at the species level. Intraspecies variation among clinical isolates and reference strains was minimal. Sixteen other pathogenic molds demonstrated less than 89% similarity withAspergillus ITS 1 and 2 sequences. A blind study of 11 clinical isolates was performed, and each was correctly identified. Clinical application of this approach may allow for earlier diagnosis and selection of effective antifungal agents for patients with IA.

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Chen, Y. C., J. D. Eisner, M. M. Kattar, et al. "Polymorphic Internal Transcribed Spacer Region 1 DNA Sequences Identify Medically Important Yeasts." Journal of Clinical Microbiology 39, no. 11 (2001): 4042–51. http://dx.doi.org/10.1128/jcm.39.11.4042-4051.2001.

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7

Carbone, Ignazio, and Linda M. Kohn. "Ribosomal DNA Sequence Divergence within Internal Transcribed Spacer 1 of the Sclerotiniaceae." Mycologia 85, no. 3 (1993): 415–27. http://dx.doi.org/10.1080/00275514.1993.12026293.

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8

Schmiderer, Corinna, Brigitte Lukas, Joana Ruzicka, and Johannes Novak. "What Else Is in Salviae officinalis folium? Comprehensive Species Identification of Plant Raw Material by DNA Metabarcoding." Planta Medica 84, no. 06/07 (2017): 428–33. http://dx.doi.org/10.1055/s-0043-121470.

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AbstractQuality control of drugs consists of identifying the raw material to avoid unwanted admixtures or exchange of material as well as looking for abiotic and biotic contaminations. So far, identity and microbial contamination are analyzed by separate processes and separate methods. Species identification by their DNA (“DNA barcoding”) has the potential to supplement existing methods of identification. The introduction of next-generation sequencing methods offers completely new approaches like the identification of whole communities in one analysis, termed “DNA metabarcoding”. Here we present a next-generation sequencing assessment to identify plants and fungi of two commercial sage samples (Salvia officinalis) using the standard DNA barcoding region “internal transcribed spacer” consisting of internal transcribed spacer 1 and internal transcribed spacer 2, respectively. The main species in both samples was identified as S. officinalis. The spectrum of accompanying plant and fungal species, however, was completely different between the samples. Additionally, the composition between internal transcribed spacer 1 and internal transcribed spacer 2 within the samples was different and demonstrated the influence of primer selection and therefore the need for harmonization. This next-generation sequencing approach does not result in quantitative species composition but gives deeper insight into the composition of additional species. Therefore, it would allow for a better knowledge-based risk assessment than any other method available. However, the method is only economically feasible in routine analysis if a high sample throughput can be guaranteed.

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9

Fan, Congzhao, Xiaojin Li, Jun Zhu, Jingyuan Song, and Hui Yao. "Endangered Uyghur Medicinal PlantFerulaIdentification through the Second Internal Transcribed Spacer." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/479879.

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The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.

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10

Kim, Jae-Seok, Yong-Kyun Kim, Ji Young Park, et al. "Analysis of Internal Transcribed Spacer 1 Sequences of Pneumocystis jiroveci from Clinical Specimens." Chonnam Medical Journal 44, no. 2 (2008): 82. http://dx.doi.org/10.4068/cmj.2008.44.2.82.

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11

Holmdahl, O. J. M., and J. G. Mattsson. "Rapid and sensitive identification ofNeospora caninumbyin vitroamplification of the internal transcribed spacer 1." Parasitology 112, no. 2 (1996): 177–82. http://dx.doi.org/10.1017/s0031182000084742.

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SummaryNeospora caninumandN. caninum-like organisms are cyst-forming coccidian parasites known to cause neuromuscular disorders in dogs and abortion in cattle. In this article we report on the use of the polymerase chain reaction (PCR) for the detection of DNA fromN. caninum. After determining the sequence of the internal transcribed spacer 1 (ITSl) ofN. caninumandToxoplasma gondii, and part of the sequences for & species ofSarcocystis, we designed a primer set for the amplification of a 279-base-pair fragment of ITSl fromN. caninum. The PCR system made possible the specific detection of 5N. caninumorganisms and no amplification was observed from any of the other cyst-forming coccidia tested, including the closely relatedT. gondii. Furthermore, we were also able to demonstrate the presence ofN. caninumin brain and lung tissue samples from experimentally infected mice. Our data also link the 5-8S rRNA gene forT. gondiiandN. caninumto the 16S-like rRNA gene, within the rDNA unit.

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12

Szalanski, A. L., J. W. Austin, J. A. McKern, C. D. Steelman, and R. E. Gold. "Mitochondrial and Ribosomal Internal Transcribed Spacer 1 Diversity of Cimex lectularius (Hemiptera: Cimicidae)." Journal of Medical Entomology 45, no. 2 (2008): 229–36. http://dx.doi.org/10.1093/jmedent/45.2.229.

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13

Das, Koushik, Punam Chowdhury, and Sandipan Ganguly. "Internal Transcribed Spacer 1 (ITS1) based sequence typing reveals phylogenetically distinct Ascaris population." Computational and Structural Biotechnology Journal 13 (2015): 478–83. http://dx.doi.org/10.1016/j.csbj.2015.08.006.

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14

Liu, Jih-Shiou, and Christopher L. Schardl. "A conserved sequence in internal transcribed spacer 1 of plant nuclear rRNA genes." Plant Molecular Biology 26, no. 2 (1994): 775–78. http://dx.doi.org/10.1007/bf00013763.

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15

Zhuo, Lang, S. L. Sajdak, and R. B. Phillips. "Minimal intraspecific variation in the sequence of the transcribed spacer regions of the ribosomal DNA of lake trout (Salvelinus namaycush)." Genome 37, no. 4 (1994): 664–71. http://dx.doi.org/10.1139/g94-094.

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Intraspecific variation in the sequence of the transcribed spacer regions of the ribosomal DNA (rDNA) in lake trout was examined by restriction mapping and sequencing of these regions amplified by the polymerase chain reaction. The length of the first internal transcribed spacer region (ITS-1) was 566 bases and the second internal transcribed spacer region (ITS-2) was 368 bases in lake trout. When the 1.4-kb region including the ITS-1, the 5.8S coding region, and the ITS-2 was amplified from 12 individuals from four populations and digested with eight different enzymes only one intraindividual polymorphism was found that occurred in each population. When the amplified ITS-1 region was sequenced from an additional 10 individuals from five populations, no interindividual variation was found in the sequence. A 6-kb portion of the rDNA repeat unit including 1.6 kb of the 18S coding region, the 5′ external spacer region (5′ ETS), and part of the adjacent intergenic spacer was cloned and a restriction map was prepared for these regions in lake trout. No intraspecific variation was found in the region adjacent to the 18S rDNA, which includes the 5′ ETS, although intraspecific and intraindividual length variation was found in the intergenic spacer region 3–6 kb from the 18S. Sequencing of a 609-b segment of the 5′ ETS adjacent to the 18S coding region revealed the presence of two 41-b repeats. The 198-b sequence between the repeats had some similarity to the 18S coding region of other fishes. Primers were designed for amplification of 559 b of the 5′ ETS using the polymerase chain reaction. No intraspecific variation in this region in lake trout was found when the DNA amplified from this region in 12 individuals from four populations was digested with eight restriction enzymes.Key words: ribosomal DNA, internal transcribed spacer regions, 5′ external spacer region, transcribed spacer, lake trout.

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16

Hinrikson, H. P., S. F. Hurst, T. J. Lott, D. W. Warnock, and C. J. Morrison. "Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species." Journal of Clinical Microbiology 43, no. 5 (2005): 2092–103. http://dx.doi.org/10.1128/jcm.43.5.2092-2103.2005.

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17

Rohimah, S., T. Ratnasari, and M. Su’udi. "DNA barcoding of Thrixspermum longipilosum based on Internal Transcribed Spacer 2 (ITS2) region." IOP Conference Series: Earth and Environmental Science 743, no. 1 (2021): 012092. http://dx.doi.org/10.1088/1755-1315/743/1/012092.

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18

Tomar, Swati, Kamlesh Malik, Seema Sharma, Mehi Lal, S. K. Kaushik, and Birpal Singh. "The Internal Transcribed Spacer 1 Region, A Quick Tool for Molecular Identification ofBemisia tabaci." Vegetos- An International Journal of Plant Research 26, no. 2s (2013): 160. http://dx.doi.org/10.5958/j.2229-4473.26.2s.135.

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19

Liqiang, ZHONG, ZHANG Chengfeng, ZHOU Kai, LI Bing, WANG Jianxin, and ZHU Jian. "Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of four common carp populations." Journal of Lake Sciences 23, no. 2 (2011): 271–76. http://dx.doi.org/10.18307/2011.0217.

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20

Thornhill, Daniel J., and Jenna B. Lord. "Secondary Structure Models for the Internal Transcribed Spacer (ITS) Region 1 from Symbiotic Dinoflagellates." Protist 161, no. 3 (2010): 434–51. http://dx.doi.org/10.1016/j.protis.2009.11.004.

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21

Santamaria, Monica, Bruno Fosso, Flavio Licciulli, et al. "ITSoneDB: a comprehensive collection of eukaryotic ribosomal RNA Internal Transcribed Spacer 1 (ITS1) sequences." Nucleic Acids Research 46, no. D1 (2017): D127—D132. http://dx.doi.org/10.1093/nar/gkx855.

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22

Perera, Omaththage P., Kerry C. Allen, Devendra Jain, Matthew Purcell, Nathan S. Little, and Randall G. Luttrell. "Rapid Identification ofHelicoverpa armigeraandHelicoverpa zea(Lepidoptera: Noctuidae) Using Ribosomal RNA Internal Transcribed Spacer 1." Journal of Insect Science 15, no. 1 (2015): 155. http://dx.doi.org/10.1093/jisesa/iev137.

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23

Van Wormhoudt, Alain, Mehdi Adjeroud, Heloise Rouzé, and Matthieu Leray. "Recent and old duplications in crustaceans “Internal Transcribed Spacer 1″: structural and phylogenetic implications." Molecular Biology Reports 46, no. 5 (2019): 5185–95. http://dx.doi.org/10.1007/s11033-019-04976-4.

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24

JIANG, XIAO-DIE, FANG-RU NAN, JUN-PING LV, et al. "Dinobryon taiyuanensis (Chrysophyta, Dinobryaceae), a new freshwater species described from Shanxi province, China." Phytotaxa 404, no. 1 (2019): 41. http://dx.doi.org/10.11646/phytotaxa.404.1.4.

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A new freshwater species of Chrysophyceae, Dinobryon taiyuanensis, is described from Linde Lake in Shanxi province, China. This new species is similar to D. sertularia, the type species of the genus, in the shape of the lorica and organization of the colony. The cells have two heterokont flagella surrounded by a lorica and occurred both as free-living, solitary cells or in branched colonies. The lorica of our new species like a bent or S-shaped cone, and shorter than the lorica in D. sertularia. In addition to describing the morphological features of D. taiyuanensis, a phylogenetic analysis based on sequences of the nuclear small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer (including internal transcribed spacer 1, 5.8S rDNA and internal transcribed spacer 2) placed this alga in single clade with a considerable sequence distance from the other Dinobryon species. Thus, results of both morphological comparisons and phylogenetic analysis based on molecular data suggest this alga as a new species, increasing the total number of recognized freshwater Chrysophyta species in China.

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25

Saenz, Gregory S., and John W. Taylor. "Phylogeny of the Erysiphales (powdery mildews) inferred from internal transcribed spacer ribosomal DNA sequences." Canadian Journal of Botany 77, no. 1 (1999): 150–68. http://dx.doi.org/10.1139/cjb-77-1-150.

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26

LI, GUO-QING, XIAO-FENG XUE, KAI-JUN ZHANG, and XIAO-YUE HONG. "Identification and molecular phylogeny of agriculturally important spider mites (Acari: Tetranychidae) based on mitochondrial and nuclear ribosomal DNA sequences, with an emphasis on Tetranychus." Zootaxa 2647, no. 1 (2010): 1. http://dx.doi.org/10.11646/zootaxa.2647.1.1.

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Tetranychid mites are serious agricultural pests. Identification of species in the Tetranychidae is hampered by their close morphological similarities, especially for species within the genus Tetranychus. In this study, we examined the relationships of nine agriculturally important species in the Tetranychidae from China based on mitochondrial (cytochrome c oxidase subunit 1) and nuclear (internal transcribed spacer 1 and 2 of ribosomal RNA gene) sequences. The results confirm the monophyly of the morphologically defined Tetranychus, Panonychus, Amphitetranychus and Petrobia. However the position of Amphitetranychus viennensis within the Tetranychidae needs to be confirmed. The genetic distances between Tetranychus truncatus, T. turkestani and T. urticae that their taxonomy needs revision. In particular, both cytochrome oxidase 1 and the internal transcribed spacers 1 and 2 of rDNA sequences showed large geographical differences within T. cinnabarinus, suggesting the existence of cryptic species within this species.

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Lieckfeldt, Elke, Gary J. Samuels, Thomas Börner, and Walter Gams. "Trichoderma koningii: neotypification and Hypocrea teleomorph." Canadian Journal of Botany 76, no. 9 (1998): 1507–22. http://dx.doi.org/10.1139/b98-090.

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A neotype is selected for Trichoderma koningii. The species is characterized by morphological and molecular characters, the latter including polymerase chain reaction (PCR) fingerprinting and restriction fragment length polymorphism (RFLP) analysis and direct sequencing of the variable regions of the rDNA (internal transcribed spacer 1 (ITS-1) and internal transcribed spacer (ITS-2)). The neotype was selected from among four cultures obtained from soil at the type locality. These cultures were compared with a broader collection of Trichoderma strains and anamorphs of Hypocrea that morphologically conform to T. koningii. Comparison was also made with members of Trichoderma sect. Trichoderma and of the Hypocrea schweinitzii complex (Trichoderma sect. Longibrachiatum). According to its ITS sequences, T. koningii is a member of sect. Trichoderma, showing very low variability in comparison with Trichoderma viride and Trichoderma atroviride. We found five additional strains from various geographical regions that are identical to the neotype in morphology and in their sequences. One of them is Hypocrea koningii sp.nov., which we consider to be the teleomorph of T. koningii. PCR fingerprint patterns demonstrate the high genetic similarity of these nine strains. There was only low similarity in molecular characters between T. koningii and the H. schweinitzii complex despite morphological similarities.Key words: Hypocreales, Hypocrea muroiana, Hypocrea rufa, internal transcribed spacer, systematics.

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28

Sarkhel, Souti Prasad, Surender Kumar Gupta, Jyoti Kaushik, et al. "Molecular characterization of internal transcribed spacer 1 (ITS 1) region of different Trypanosoma evansi isolates of India." Journal of Parasitic Diseases 41, no. 2 (2016): 527–33. http://dx.doi.org/10.1007/s12639-016-0843-9.

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29

Tian, Zhancheng, Guangyuan Liu, Junren Xie, et al. "The internal transcribed spacer 1 (ITS-1), a controversial marker for the genetic diversity of Trypanosoma evansi." Experimental Parasitology 129, no. 3 (2011): 303–6. http://dx.doi.org/10.1016/j.exppara.2011.08.006.

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30

Newton, L. "Differences in the second internal transcribed spacer of four species of Nematodirus (Nematoda: Molineidae)." International Journal for Parasitology 28, no. 2 (1998): 337–41. http://dx.doi.org/10.1016/s0020-7519(97)00150-1.

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31

Marsh, A. E., B. C. Barr, L. Tell, et al. "Comparison of the Internal Transcribed Spacer, ITS-1, from Sarcocystis falcatula Isolates and Sarcocystis neurona." Journal of Parasitology 85, no. 4 (1999): 750. http://dx.doi.org/10.2307/3285758.

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32

Campbell, Christopher S., Wesley A.Wright, Margaret Cox, Thomas F. Vining, C. Smoot Major, and Matthew P. Arsenault. "Nuclear ribosomal DNA internal transcribed spacer 1 (ITS1) in Picea (Pinaceae): sequence divergence and structure." Molecular Phylogenetics and Evolution 35, no. 1 (2005): 165–85. http://dx.doi.org/10.1016/j.ympev.2004.11.010.

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33

Homan, W. L., Louis Limper, Marijke Verlaan, Annemarie Borst, Martine Vercammen, and Frans van Knapen. "Comparison of the internal transcribed spacer, ITS 1, from Toxoplasma gondii isolates and Neospora caninum." Parasitology Research 83, no. 3 (1997): 285–89. http://dx.doi.org/10.1007/s004360050248.

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34

Szalanski, Allen L., James W. Austin, Jackie A. McKern, C. Dayton Steelman, and Roger E. Gold. "Mitochondrial and Ribosomal Internal Transcribed Spacer 1 Diversity of Cimex lectularius (Hemiptera: Cimicidae)." Journal of Medical Entomology 45, no. 2 (2008): 229–36. http://dx.doi.org/10.1603/0022-2585(2008)45[229:marits]2.0.co;2.

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35

Mahmud, Md Muket, Jayedul Hassan, and KHM Nazmul Hussain Nazir. "Internal transcribed spacer based identification of Aspergillus fumigatus isolated from poultry feed samples." Research in Agriculture Livestock and Fisheries 4, no. 3 (2017): 165–71. http://dx.doi.org/10.3329/ralf.v4i3.35093.

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Among over 180 Aspergilli, Aspergillus fumigatus is the most common etiological agent causing invasive mold infection mostly in immunocompromised human and animal. Besides, the fungus is used for various useful purposes. However, for the utilization of A. fumigatus as a useful candidate, accurate identification is crucial. Here, the research work was aimed at identifying A. fumigatus from poultry feed samples using conventional and molecular techniques. Out of 23 feed samples, 2 (8.7%) were found to be positive for A. fumigatus. The internal transcribed spacer 1 (ITS 1) and ITS 2 regions and the 5.8S ribosomal DNA (rDNA) region of the fungus were amplified by polymerase chain reaction. The ITS regions are located between the 18S and 28S rRNA genes, and rRNA gene for 5.8S RNA separates these two ITS regions. The isolated gene has been sequenced and deposited in the GenBank (accession no. KC142152). The gene was 100% similar to other reference species of A. fumigatus, whereas in phylogenetic analysis, a clear distance was found in the cases of other Aspergilli. Based on the unique nature of the ITS1 and ITS2 regions and phylogenetic analysis of the genes, A. fumigatus was correctly identified. The isolated strain could be a good candidate for further studies especially for utilization in the field of biotechnology.Res. Agric. Livest. Fish.4(3): 165-171, December 2017

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Martins, L., C. Oberprieler, and F. H. Hellwig. "A phylogenetic analysis of Primulaceae s.l. based on internal transcribed spacer (ITS) DNA sequence data." Plant Systematics and Evolution 237, no. 1-2 (2003): 75–85. http://dx.doi.org/10.1007/s00606-002-0258-1.

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Shrestha, Sangita, Stephen W. Adkins, Glenn C. Graham, and Donald S. Loch. "Phylogeny of the Sporobolus indicus complex, based on internal transcribed spacer (ITS) sequences." Australian Systematic Botany 16, no. 2 (2003): 165. http://dx.doi.org/10.1071/sb02009.

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The entire internal transcribed spacer (ITS) region, including the 5.8S subunit of the nuclear ribosomal DNA (rDNA), was sequenced by direct double-stranded sequencing of polymerase chain reaction (PCR) amplified fragments. The study included 40 Sporobolus (Family Poaceae, subfamily Chloridoideae) seed collections from 14 putative species (all 11 species from the S. indicus complex and three Australian native species). These sequences, along with those from two out-group species [Pennisetum alopecuroides (L.) Spreng. and Heteropogon contortus (L.) P. Beauv. ex Roemer & Schultes, Poaceae, subfamily Panicoideae], were analysed by the parsimony method (PAUP; version 4.0b4a) to infer phylogenetic relationships among these species. The length of the ITS1, 5.8S subunit and ITS2 region were 222, 164 and 218 base pairs (bp), respectively, in all species of the S. indicus complex, except for the ITS2 region of S. diandrus P.Beauv. individuals, which was 217 bp long. Of the 624 characters included in the analysis, 245 (39.3%) of the 330 variable sites contained potential phylogenetic information. Differences in sequences among the members of the S. pyramidalis P.Beauv., S. natalensis (Steud.) Dur & Schinz and S. jacquemontii Kunth. collections were 0%, while differences ranged from 0 to 2% between these and other species of the complex. Similarly, differences in sequences among collections of S. laxus B.K.Simon, S. sessilis B.K.Simon, S. elongatus R.Br. and S. creber De Nardi were 0%, compared with differences of 1–2% between these four species and the rest of the complex. When comparing S. fertilis (Steud.) Clayton and S. africanus (Poir.) Robyns & Tourney, differences between collections ranged from 0 to 1%. Parsimony analysis grouped all 11 species of the S.�indicus complex together, indicating a monophyletic origin. For the entire data set, pair-wise distances among members of the S. indicus complex varied from 0.00 to 1.58%, compared with a range of 20.08–21.44% among species in the complex and the Australian native species studied. A strict consensus phylogenetic tree separated 11 species of the S. indicus complex into five major clades. The phylogeny, based on ITS sequences, was found to be congruent with an earlier study on the taxonomic relationship of the weedy Sporobolus grasses revealed from random amplified polymorphic DNA (RAPD). However, this cladistic analysis of the complex was not in agreement with that created on past morphological analyses and therefore gives a new insight into the phylogeny of the S.�indicus complex.

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Güiris-Andrade, D. M., A. Oceguera-Figueroa, D. Osorio-Sarabia, et al. "Tziminema unachin. gen., n. sp. (Nematoda: Strongylidae: Strongylinae) parasite of Baird's tapirTapirus bairdiifrom Mexico." Journal of Helminthology 92, no. 6 (2017): 752–59. http://dx.doi.org/10.1017/s0022149x17001055.

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AbstractA new genus and species of nematode,Tziminema unachin. gen., n. sp. is described from the caecum and colon of Baird's tapirTapirus bairdii(Gill, 1865), found dead in the Reserva de la Biósfera El Triunfo, Chiapas State, in the Neotropical realm of Mexico.Tzimineman. gen. differs from the other nine genera included in the Strongylinae by two main characteristics: having 7–9 posteriorly directed tooth-like structures at the anterior end of the buccal capsule, and the external surface of the buccal capsule being heavily striated. Phylogenetic analyses of the DNA sequences of the mitochondrial cytochromecoxidase and nuclear DNA, including a partial sequence of the internal transcribed spacer 1, 5.8S and a partial sequence of the internal transcribed spacer 2 of the new taxon, confirmed its inclusion in Strongylinae and its rank as a new genus.

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39

Henry, Cassandra, Aravindan Kalyanasundaram, Matthew Z. Brym, and Ronald J. Kendall. "Molecular Identification of Oxyspirura Petrowi Intermediate Hosts by Nested PCR Using Internal Transcribed Spacer 1 (ITS1)." Journal of Parasitology 106, no. 1 (2020): 46. http://dx.doi.org/10.1645/19-135.

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Liu, Dong, Hong-Yi Guo, Wen-Qiao Tang, and Jin-Quan Yang. "Comparative Evolution of S7 Intron 1 and Ribosomal Internal Transcribed Spacer in Coilia nasus (Clupeiformes: Engraulidae)." International Journal of Molecular Sciences 13, no. 3 (2012): 3085–100. http://dx.doi.org/10.3390/ijms13033085.

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41

BRÄNNSTRÖM, S., D. A. MORRISON, J. G. MATTSSON, and J. CHIRICO. "Genetic differences in internal transcribed spacer 1 between Dermanyssus gallinae from wild birds and domestic chickens." Medical and Veterinary Entomology 22, no. 2 (2008): 152–55. http://dx.doi.org/10.1111/j.1365-2915.2008.00722.x.

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42

Honda, Jeffrey Y., Yoshitaka Nakashima, Tohru Yanase, Takeshi Kawarabata, and Yoshimi Hirose. "Use of the internal transcribed spacer (ITS-1) region to infer Orius (Hemiptera : Anthocoridae) species phylogeny." Applied Entomology and Zoology 33, no. 4 (1998): 567–71. http://dx.doi.org/10.1303/aez.33.567.

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Summerbell, R. C., R. A. Haugland, A. Li, and A. K. Gupta. "rRNA Gene Internal Transcribed Spacer 1 and 2 Sequences of Asexual, Anthropophilic Dermatophytes Related toTrichophyton rubrum." Journal of Clinical Microbiology 37, no. 12 (1999): 4005–11. http://dx.doi.org/10.1128/jcm.37.12.4005-4011.1999.

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Abstract:

The ribosomal region spanning the two internal transcribed spacer (ITS) regions and the 5.8S ribosomal DNA region was sequenced for asexual, anthropophilic dermatophyte species with morphological similarity to Trichophyton rubrum, as well as for members of the three previously delineated, related major clades in theT. mentagrophytes complex. Representative isolates ofT. raubitschekii, T. fischeri, and T. kanei were found to have ITS sequences identical to that ofT. rubrum. The ITS sequences of T. soudanenseand T. megninii differed from that of T. rubrumby only a small number of base pairs. Their continued status as species, however, appears to meet criteria outlined in the population genetics-based cohesion species concept of A. R. Templeton. The ITS sequence of T. tonsurans differed from that of the biologically distinct T. equinum by only 1 bp, while the ITS sequence of the recently described species T. krajdeniihad a sequence identical to that of T. mentagrophytesisolates related to the teleomorph Arthroderma vanbreuseghemii.

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Lindahl, Lasse, Richard H. Archer, and Janice M. Zengel. "Alternate pathways for processing in the internal transcribed spacer 1 in pre-rRNA of Saccharomyces cerevisiae." Nucleic Acids Research 22, no. 24 (1994): 5399–407. http://dx.doi.org/10.1093/nar/22.24.5399.

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Marks, Jane C., and Michael P. Cummings. "DNA SEQUENCE VARIATION IN THE RIBOSOMAL INTERNAL TRANSCRIBED SPACER REGION OF FRESHWATER CLADOPHORA SPECIES (CHLOROPHYTA)1." Journal of Phycology 32, no. 6 (1996): 1035–42. http://dx.doi.org/10.1111/j.0022-3646.1996.01035.x.

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Koetschan, Christian, Sandra Kittelmann, Jingli Lu, et al. "Internal Transcribed Spacer 1 Secondary Structure Analysis Reveals a Common Core throughout the Anaerobic Fungi (Neocallimastigomycota)." PLoS ONE 9, no. 3 (2014): e91928. http://dx.doi.org/10.1371/journal.pone.0091928.

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Mohamed, A. M., D. J. Kuyper, P. C. Iwen, H. H. Ali, D. R. Bastola, and S. H. Hinrichs. "Computational Approach Involving Use of the Internal Transcribed Spacer 1 Region for Identification of Mycobacterium Species." Journal of Clinical Microbiology 43, no. 8 (2005): 3811–17. http://dx.doi.org/10.1128/jcm.43.8.3811-3817.2005.

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Stevens, A., C. L. Hsu, K. R. Isham, and F. W. Larimer. "Fragments of the internal transcribed spacer 1 of pre-rRNA accumulate in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1." Journal of Bacteriology 173, no. 21 (1991): 7024–28. http://dx.doi.org/10.1128/jb.173.21.7024-7028.1991.

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Siniscalco Gigliano, G. "Identification of Cannabis sativa L. (Cannabaceae) using restriction profiles of the Internal Transcribed Spacer II (ITS2)." Science & Justice 38, no. 4 (1998): 225–30. http://dx.doi.org/10.1016/s1355-0306(98)72116-1.

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Jatmiko, Y. D., G. S. Howarth, and M. D. Barton. "Evaluation of Yeast Diversity in Dadih and Dangke Using PCR-RFLP of Internal Transcribed Spacer Region." IOP Conference Series: Earth and Environmental Science 391 (December 19, 2019): 012025. http://dx.doi.org/10.1088/1755-1315/391/1/012025.

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