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Relevant bibliographies by topics / Internal Transcribed Spacer 2

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Academic literature on the topic 'Internal Transcribed Spacer 2'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

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Journal articles on the topic "Internal Transcribed Spacer 2"

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Wang, Chuan Tang, Xiu Zhen Wang, Yue Yi Tang, Dian Xu Chen, Feng Gao Cui, Jian Cheng Zhang, and Shan Lin Yu. "Phylogeny of Arachis based on internal transcribed spacer sequences." Genetic Resources and Crop Evolution 58, no. 2 (June 9, 2010): 311–19. http://dx.doi.org/10.1007/s10722-010-9576-2.

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Voronov, A. S., D. V. Shibalev, A. P. Ryskov, and N. S. Kupriyanova. "Evolutionary divergence of ribosomal internal transcribed spacer 2 in lizards." Molecular Biology 40, no. 1 (January 2006): 37–42. http://dx.doi.org/10.1134/s0026893306010079.

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Ji, Lei, Chunju Liu, Li Zhang, Aixin Liu, and Jinfeng Yu. "Variation of rDNA Internal Transcribed Spacer Sequences in Rhizoctonia cerealis." Current Microbiology 74, no. 7 (May 5, 2017): 877–84. http://dx.doi.org/10.1007/s00284-017-1258-2.

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Choi, Du Bok, Ji Lu Ding, and Wol-Suk Cha. "Homology search of genus Pleurotus using an internal transcribed spacer region." Korean Journal of Chemical Engineering 24, no. 3 (May 2007): 408–12. http://dx.doi.org/10.1007/s11814-007-0070-2.

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Schmiderer, Corinna, Brigitte Lukas, Joana Ruzicka, and Johannes Novak. "What Else Is in Salviae officinalis folium? Comprehensive Species Identification of Plant Raw Material by DNA Metabarcoding." Planta Medica 84, no. 06/07 (November 17, 2017): 428–33. http://dx.doi.org/10.1055/s-0043-121470.

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AbstractQuality control of drugs consists of identifying the raw material to avoid unwanted admixtures or exchange of material as well as looking for abiotic and biotic contaminations. So far, identity and microbial contamination are analyzed by separate processes and separate methods. Species identification by their DNA (“DNA barcoding”) has the potential to supplement existing methods of identification. The introduction of next-generation sequencing methods offers completely new approaches like the identification of whole communities in one analysis, termed “DNA metabarcoding”. Here we present a next-generation sequencing assessment to identify plants and fungi of two commercial sage samples (Salvia officinalis) using the standard DNA barcoding region “internal transcribed spacer” consisting of internal transcribed spacer 1 and internal transcribed spacer 2, respectively. The main species in both samples was identified as S. officinalis. The spectrum of accompanying plant and fungal species, however, was completely different between the samples. Additionally, the composition between internal transcribed spacer 1 and internal transcribed spacer 2 within the samples was different and demonstrated the influence of primer selection and therefore the need for harmonization. This next-generation sequencing approach does not result in quantitative species composition but gives deeper insight into the composition of additional species. Therefore, it would allow for a better knowledge-based risk assessment than any other method available. However, the method is only economically feasible in routine analysis if a high sample throughput can be guaranteed.

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Henry, Travis, Peter C. Iwen, and Steven H. Hinrichs. "Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2." Journal of Clinical Microbiology 38, no. 4 (2000): 1510–15. http://dx.doi.org/10.1128/jcm.38.4.1510-1515.2000.

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Aspergillus species are the most frequent cause of invasive mold infections in immunocompromised patients. Although over 180 species are found within the genus, 3 species, Aspergillus flavus, A. fumigatus, and A. terreus, account for most cases of invasive aspergillosis (IA), with A. nidulans, A. niger, and A. ustus being rare causes of IA. The ability to distinguish between the various clinically relevant Aspergillus species may have diagnostic value, as certain species are associated with higher mortality and increased virulence and vary in their resistance to antifungal therapy. A method to identify Aspergillus at the species level and differentiate it from other true pathogenic and opportunistic molds was developed using the 18S and 28S rRNA genes for primer binding sites. The contiguous internal transcribed spacer (ITS) region, ITS 1–5.8S–ITS 2, from referenced strains and clinical isolates of aspergilli and other fungi were amplified, sequenced, and compared with non-reference strain sequences in GenBank. ITS amplicons fromAspergillus species ranged in size from 565 to 613 bp. Comparison of reference strains and GenBank sequences demonstrated that both ITS 1 and ITS 2 regions were needed for accurate identification ofAspergillus at the species level. Intraspecies variation among clinical isolates and reference strains was minimal. Sixteen other pathogenic molds demonstrated less than 89% similarity withAspergillus ITS 1 and 2 sequences. A blind study of 11 clinical isolates was performed, and each was correctly identified. Clinical application of this approach may allow for earlier diagnosis and selection of effective antifungal agents for patients with IA.

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Zhao, Li-Li, Shi-Jing Feng, Jie-Yun Tian, An-Zhi Wei, and Tu-Xi Yang. "Internal transcribed spacer 2 (ITS2) barcodes: A useful tool for identifying ChineseZanthoxylum." Applications in Plant Sciences 6, no. 6 (June 2018): e01157. http://dx.doi.org/10.1002/aps3.1157.

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van der Sande, Carine A. F. M., Marcel Kwa, Rob W. van Nues, Harm van Heerikhuizen, Hendrik A. Raué, and Rudi J. Planta. "Functional analysis of internal transcribed spacer 2 of Saccharomyces cerevisiae ribosomal DNA." Journal of Molecular Biology 223, no. 4 (February 1992): 899–910. http://dx.doi.org/10.1016/0022-2836(92)90251-e.

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Fan, Congzhao, Xiaojin Li, Jun Zhu, Jingyuan Song, and Hui Yao. "Endangered Uyghur Medicinal PlantFerulaIdentification through the Second Internal Transcribed Spacer." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/479879.

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The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.

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Inglis, Peter, Lorena Mata, Marcos da Silva, Roberto Vieira, Rosa de B. N. Alves, Dijalma Silva, and Vânia Azevedo. "DNA Barcoding for the Identification of Phyllanthus Taxa Used Medicinally in Brazil." Planta Medica 84, no. 17 (June 21, 2018): 1300–1310. http://dx.doi.org/10.1055/a-0644-2688.

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AbstractPlants of the genus Phyllanthus, principally Phyllanthus amarus, Phyllanthus urinaria, Phyllanthus niruri, and Phyllanthus tenellus, are used in Brazilian folk medicine to treat kidney stones as well as other ailments, where the latter two species are listed in the Brazilian Pharmacopeia as quebra-pedra (stone-breaker). However, only P. niruri has been shown to be effective in a clinical setting. Nuclear ribosomal internal transcribed spacer (ITS1 – 5.8S rRNA-ITS2), internal transcribed spacer 2, and chloroplasts rbcL, matK, psbA-trnH, trnL, and trnL-trnF were screened for their potential as DNA barcodes for the identification of 48 Phyllanthus taxa in Brazilian medicinal plant germplasm banks and in “living pharmacies”. The markers were also tested for their ability to validate four commercial herbal teas labelled as quebra-pedra. Using the criterion of high clade posterior probability in Bayesian phylogenetic analysis, the internal transcribed spacer, internal transcribed spacer 2, and chloroplast matK, psbA-trnH, trnL, and trnL-trnF markers all reliably differentiated the four Phyllanthus species, with the internal transcribed spacer and matK possessing the additional advantage that the genus is well represented for these markers in the Genbank database. However, in the case of rbcL, posterior probability for some clades was low and while P. amarus and P. tenellus formed monophyletic groups, P. niruri and P. urinaria accessions could not be reliably distinguished with this marker. Packaged dried quebra-pedra herb from three Brazilian commercial suppliers comprised P. tenellus, but one sample was also found to be mixed with alfalfa (Medicago sativa). An herb marketed as quebra-pedra from a fourth supplier was found to be composed of a mixture of Desmodium barbatum and P. niruri.

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Dissertations / Theses on the topic "Internal Transcribed Spacer 2"

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Viswanathan, Aparna. "Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2." Texas A&M University, 2003. http://hdl.handle.net/1969/394.

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Förster, Frank [Verfasser], and Thomas [Akademischer Betreuer] Dandekar. "Making the most of phylogeny: Unique adaptations in tardigrades and 216374 internal transcribed spacer 2 structures / Frank Förster. Betreuer: Thomas Dandekar." Würzburg : Universitätsbibliothek der Universität Würzburg, 2012. http://d-nb.info/1022061232/34.

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Tozkar, Ozge Cansu. "Comparative Sequence Analysis Of The Internal Transcribed Spacer 2 Region Of Turkish Red Pine (pinus Brutia Ten.) And Natural Aleppo Pine (pinus Halepensis Mill.) Populations From Turkey." Master's thesis, METU, 2007. http://etd.lib.metu.edu.tr/upload/2/12608313/index.pdf.

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ABSTRACT COMPARATIVE SEQUENCE ANALYSIS OF THE INTERNAL TRANSCRIBED SPACER 2 REGION OF TURKISH RED PINE (Pinus brutia TEN.) AND NATURAL ALEPPO PINE (Pinus halepensis MILL.) POPULATIONS FROM TURKEY Tozkar, Ö
zge M.S., Department of Biology Supervisor: Prof. Dr. Zeki Kaya April, 2007, 107 pages Turkish red pine (Pinus brutia) is wide-spread and an important forest tree species in Turkey, occurring mainly in southern, western and north-western Turkey and as small isolated populations in the Black Sea region. Aleppo pine (Pinus halepensis) has naturally found only in Adana and Mugla provinces as small population in mixture with Turkish red pine. Although Turkish red pine and Aleppo pine are morphologically different, Turkish red pine has been regarded as subspecies of Aleppo pine by some taxonomists due to occurrence of natural hybridization between these two species. However, the phylogenic relationship between these species needs to be explored further. In the present study, by sampling overlapped populations of both species from Mugla and Adana provinces (4 populations of Turkish red pine and 3 populations of Aleppo pine), internal transcribed spacer (ITS) region of ribosomal DNA were comparatively studied with sequence analysis. Although ITS1, 5.8s and ITS2 regions of ribosomal DNA were studied with ITS primers, only ITS2 region was successfully amplified with polymerase chain reaction (PCR). The complete data set for this region was analysed using MEGA3.1 and Arlequin softwares. Analysis of molecular variance (AMOVA) demonstrated the highest genetic differentiation between Turkish red pine and Aleppo pine in Mugla with 100 percentage of variation. AMOVA analysis also indicated the possibility of low-level migration of genes between Turkish red pine and Aleppo pine populations in Adana with 50.65 percent of molecular variance. Haplotype comparison revealed that two major haplotypes were represented Based on the results of ITS2 region sequence analysis, Turkish populations of Aleppo pine and Turkish red pine populations could not be fully differentiated. In Mugla province Turkish red pine and Aleppo pine revealed more differentiation due to reproductive isolation. But in Adana province, two species shared more common genetic background due to possible hybridization. Since ITS2 region of nuclear ribosomal DNA revealed a few variable and parsimony informative sites for both species, thus, only ITS2 region of ribosomal DNA does not appear to be sufficient for fully resolving genetic relationships between Turkish red pine and Aleppo pine populations. Further studies including ITS1 and 5.8s regions of ribosomal DNA and populations included from major Aleppo pine distribution areas will be useful to understand the evolutionary relationship between Aleppo pine and Turkish red pine populations in Turkey.

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Cheung, Mei, and 張微. "Internal transcribed spacer as the DNA barcode for pathogenic fungi." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/206495.

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Identification of pathogenic fungi isolated from clinical specimens in clinical microbiology laboratories is primarily based on observing fungal phenotypic structures under the microscope and performing biochemical tests for fungal cultures. This conventional method is very time-consuming and labor-dependent. It usually requires several weeks for the fungi to grow sufficiently on culture media, and the identification processes on fungal phenotypic structure rely very much on experienced staff. Therefore, a more accurate and rapid method for pathogenic fungal identification is necessary for clinical laboratories to get abreast of modern development. Gene sequencing and phylogenetic analysis targeting the internal transcribed spacer (ITS) region in the fungal genomes are the most commonly used molecular methods for fungal identification. Because of the optimal inter and intra-species variation property of the ITS region, it can act as the DNA barcode to identify fungi to the species level. In this study, 33 clinical fungal isolates were identified by both phenotypic method and ITS sequencing. The results showed that 23 isolates were successfully identified to thespecies level by both phenotypic and molecular methods. Moreover, five isolates were only identified to the genus level by phenotypic method, but they could be successfully identified to the species level by ITS sequencing. However, five isolates have not been differentiated because there were mismatched results from phenotypic and sequencing methods. It may be due to the limitation of sequencing method on some fungal species. Building up a more comprehensive database or setting up a standard platform to guide the molecular process may help improve the performance of molecular method. To conclude, molecular method is a rapid and reliable way for fungal identification because ITS region acts as the DNA barcode for pathogenic fungi.
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Bodo, Slotta Tracey A. "Phylogenetic Analysis of Iliamna (Malvaceae) Using the Internal Transcribed Spacer Region." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/33211.

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The genus Iliamna Greene has a taxonomically complex history. Since its desciption in 1906, the genus was not recognized for some time, several species were initially placed into other genera, and the species status of a few was questioned. Today, eight species of Iliamna are recognized. Six species are located in western North America and two are found isolated to the east. Species in Iliamna are very similar morphologically with only a few characters distinguishing several as separate entities. The need for systematic study became apparent since all but one species are considered rare or endangered. Also, the differentiation between two endangered species, I. corei and I. remota, was unclear in a previous study using random amplified polymorphic DNA fragments. Of the western species, four overlap in distribution (I. crandallii, I grandi ora, I. longisepala, and I. rivularis) and their recognition as separate species has been questioned. The focus of this study was to develop a phylogeny for Iliamna using sequences from the internal transcribed spacer region (ITS) of the nuclear ribosomal RNA genes in order to determine its biogeographical and evolutionary history. Cladistic analysis was performed and the resulting phylogeny is presented. The ITS data provide new insights in the origination of the genus and its distribution. In Iliamna, the ITS region is 677 base pairs long with 120 sites providing information in the formation of phylogenetic trees. Iliamna forms a well-supported clade distinct from related genera and is monophyletic. Three well-supported groups are formed. One contains representatives from the Pacific Northwest. Another contains all of the remaining species with the third clade nested therein. This last clade contains the two eastern species, I. corei and I. remota, but there is not enough variability to support the divergence of these taxa as distinct species. There is also not sufficient variability in the ITS region to identify the western species I. crandallii, I. grandi ora, I. longisepala and I. rivularis as distinct entities.
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Hao, Gang, and 郝剛. "Molecular phylogeny of the illiciales based on internal transcribed spacer sequences of ribosomal DNA." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1999. http://hub.hku.hk/bib/B31238567.

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Hao, Gang. "Molecular phylogeny of the illiciales based on internal transcribed spacer sequences of ribosomal DNA /." Hong Kong : University of Hong Kong, 1999. http://sunzi.lib.hku.hk/hkuto/record.jsp?B21029027.

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Ragozine, Vincent. "Analyses of ribosomal DNA internal transcribed spacer sequences from : Juglans nigra and leaf-associated fungi in Zoar Valley, NY /." Connect to resource online, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1211685563.

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Ragozine, Vincent Kyle. "Analyses of ribosomal DNA internal transcribed spacer sequences from Juglans nigra and leaf-associated fungi in Zoar Valley, NY." Youngstown State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1211685563.

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Heidemann, Stefan [Verfasser]. "DNA-Polymorphismen der internal transcribed spacer-Region zur Differenzierung anthropophiler und zoophiler Spezies von Trichophyton mentagrophytes sensu lato / Stefan Heidemann." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2010. http://d-nb.info/1024334767/34.

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Books on the topic "Internal Transcribed Spacer 2"

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Grubisha, Lisa C. Systematics of the genus Rhizopogon inferred from nuclear ribosomal DNA large subunit and internal transcribed spacer sequences. 1998.

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Abdennadher, Mourad. Molecular fingerprinting, rDNA internal transcribed spacer sequence, and karyotype analysis of Ustilago hordei and related smut fungi. 1995.

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Moynihan, Jeremy. Molecular phylogeny of the Caribbean endemic Neolaugeria (Rubiaceae), based on the internal transcribed spacer regions of nuclear ribosomal DNA. 1999.

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Book chapters on the topic "Internal Transcribed Spacer 2"

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Fujita, Shin-ichi. "Internal Transcribed Spacer (ITS)-PCR Identification of MRSA." In Methods in Molecular Biology, 97–102. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-664-1_5.

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Fujita, Shin-ichi. "Internal Transcribed Spacer (ITS)-PCR Identification of MRSA." In Methods in Molecular Biology, 51–57. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-468-1_4.

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Baayen, R. P., C. Waalwijk, and W. Gams. "Fusarium Sections Elegans and Liseola: Taxonomy, rDNA Internal Transcribed Spacer Sequences and Diagnostics." In Developments in Plant Pathology, 295–97. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-0043-1_61.

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Therese, K. Lily, R. Bagyalakshmi, and H. N. Madhavan. "Application of Polymerase Chain Reaction and PCR-Based Methods Targeting Internal Transcribed Spacer Region for Detection and Species-Level Identification of Fungi." In Laboratory Protocols in Fungal Biology, 321–30. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-2356-0_27.

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"Plant DNA Barcoding." In Advances in Environmental Engineering and Green Technologies, 133–64. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-4312-2.ch006.

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DNA barcoding has evolved as an effective species identification tool in diverse areas such as phylogeny, ecology, population genetics, and biodiversity. In this approach, a short DNA sequence from a standardized locus is employed for species identification. The technique is simple, time and cost effective, and accurate. Selection of correct DNA marker is the main criterion for success in DNA barcoding. Compared to animals, DNA barcoding is more difficult in plants, as there are multiple consensuses about selection of barcoding markers for plants DNA barcoding. Some common plant barcoding markers are chloroplast genes such as matK, rbcL, ropC1, ropB, and trnL; chloroplast intergenic specers trnH-psbA, atpF-atpH, and pdbK-psbI; and the nuclear ribosomal internal transcribed spacer (ITS). These markers can be used alone or in combinations with other markers or spacers. In this chapter, the basic requirements, selection of markers, databases, advantages, and limitations of DNA barcoding have been discussed.

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Lau, David Tai-Wai, Pang-Chui Shaw, and Paul Pui-Hay But. "Authentication of Medicinal Dendrobium Species by the Internal Transcribed Spacer of Ribosomal DNA." In Authentication of Chinese Medicinal Materials by DNA Technology, 155–68. WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812706591_0009.

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Conference papers on the topic "Internal Transcribed Spacer 2"

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M., Nor-Zuhailah, Fatihah N.H.N., Nashriyah M, Ali A.M., and Choong Chee Yen. "MOLECULAR SYSTEMATICS of Ficus deltoidea Jack (Moraceae) BASED ON INTERNAL TRANSCRIBED SPACER." In Annual International Conference on BioInformatics and Computational Biology & Annual International Conference on Advances in Biotechnology. Global Science and Technology Forum, 2011. http://dx.doi.org/10.5176/978-981-08-8119-1_biotech19.

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Hidayat, Topik, Didik Priyandoko, Dina Karina Islami, and Putri Yunitha Wardiny. "Molecular phylogenetic analysis of Indonesia Solanaceae based on DNA sequences of internal transcribed spacer region." In PROCEEDINGS OF INTERNATIONAL SEMINAR ON MATHEMATICS, SCIENCE, AND COMPUTER SCIENCE EDUCATION (MSCEIS 2015). AIP Publishing LLC, 2016. http://dx.doi.org/10.1063/1.4941148.

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"Intragenomic polymorphism of internal transcribed spacer ITS1 in the locus 35S rRNA of polyploid Avena species." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-148.

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da Silva, M. L. R. B., V. A. L. B. Cavalcanti, A. C. E. S. Mergulhão, and M. C. C. P. de Lyra. "Sequencing of the region of ribosomal internal transcribed spacer (ITS) of Metarhizium anisopliae in Pernambuco state." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0028.

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Amrullah, Muhammad Haidar, Annisa Sholikhah, Farida Aryani Dian, Elhah Nailul Khasna, I. Kade Karisma Gita Ardana, ‘Ainun Sayyidah Zakiyah, Tita Putri Milasari, Nur Diniyah, and Dwi Listyorini. "Phylogenetic study of Cantigi Ungu (Vaccinium varingifolium) based on universal internal transcribed spacer (ITSu) DNA barcoding." In THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity”. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0015822.

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Chen, Ren-Fang, Li Xu, Mao-De Yu, Xiu-Qun Liu, and Long-Qing Chen. "Determination of the Origin and Evolution of Morus (Moraceae) by Analyzing the Internal Transcribed Spacer (ITS) Sequences." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5518058.

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Mkumbe, Baraka Stewart, Sajidan, Artini Pangastuti, and Ari Susilowati. "Phylogenetic analysis based on internal transcribed spacer region (ITS1-5.8S-ITS2) of Aspergillus niger producing phytase from Indonesia." In Proceedings of the 17th International Conference on Ion Sources. Author(s), 2018. http://dx.doi.org/10.1063/1.5054419.

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Peck, Kayla, Robert Stedtfeld, Jordan RoseFigura, Brett Reed, Drew McUsic, Jon Irish, Brett Etchebarne, Timothy Johnson, Laurie Kurihara, and Vladimir Makarov. "Abstract 1484: Microbial sequencing using a single-pool target enrichment of multiple variable regions of the 16S rRNA gene, the nuclear ribosomal internal transcribed spacer (ITS) region, and antimicrobial resistance genes." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-1484.

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Peck, Kayla, Robert Stedtfeld, Jordan RoseFigura, Brett Reed, Drew McUsic, Jon Irish, Brett Etchebarne, Timothy Johnson, Laurie Kurihara, and Vladimir Makarov. "Abstract 1484: Microbial sequencing using a single-pool target enrichment of multiple variable regions of the 16S rRNA gene, the nuclear ribosomal internal transcribed spacer (ITS) region, and antimicrobial resistance genes." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-1484.

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Rosa, Marcos P., Jose V. C. Vargas, Vanessa M. Kava, Fernando G. Dias, Daiani Savi, Beatriz Santos, Wellington Balmant, Andre B. Mariano, Andre Servienski, and Juan C. Ordóñez. "Hydrogen and Compounds With Biological Activity From Microalgae." In ASME 2019 13th International Conference on Energy Sustainability collocated with the ASME 2019 Heat Transfer Summer Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/es2019-3965.

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Abstract Microalgae have a high biotechnological potential as a source of biofuels (biodiesel, biohydrogen) and other high-added value products (e.g., pharmaceuticals, proteins, pigments). However, for microalgae cultivation to be economically competitive with other fuel sources, it is necessary to apply the concept of biorefinery. This seems to be the most ambitious strategy to achieve viability. Therefore, the objectives of this study were to isolate and identify the main microalgae line used to produce biofuels at Federal University of Parana, Brazil, using the rDNA sequence and micromorphological analysis, and to evaluate the potential of this lineage in the production of hydrogen and co-products with biological activity. For the purification of the lineage (LGMM0001), an aliquot was seeded into solid CHU culture medium and an isolated colony was selected. The genomic DNA was purified using a commercial kit (Macherey-Nagel, Düren, Germany) for molecular identification, the ITS region (ITS1, 5.8S and ITS2) (Internal Transcribed Spacer) was amplified and sequenced using primers LS266 and V9G. Morphological characterization was performed as described by Hemschemeier et al. [1]. Finally, for biological activity research, secondary metabolites were extracted by fractionation and evaluated against bacteria of clinical interest. Through microscopic analysis, general characteristics shared by the genus Tetradesmus were observed. The plasticity of the morphological characteristics of this genus reinforces the need for further studies to classify correctly the species in this group, using DNA sequencing. ITS sequence analysis of LGMM0001 showed 100% homology with sequences from the Tetradesmus obliquus species, so, the lineage was classified as belonging to this species. The evaluated microalgae strain was able to produce hydrogen, showing positive results for gas formation. Biological activity was observed with the extract obtained from the residual culture carried out with alternative medium used in the photobioreactors (PBR), against the Staphylococcus aureus pathogenic lineage. In conclusion, the microalgae strain used in this work was identified as Tetradesmus obliquus (= Acutodesmus obliquus), and was able to produce a compound with economic potential in association with the existing biofuel production process.

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