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1

Carrer Filho, Renato, Renata Maria Oliveira, Vanessa Duarte Dias, Leonardo Silva Boiteux, Érico de Campos Dianese, and Marcos Gomes da Cunha. "Fontes de resistência múltipla à murcha de fusário em tomateiro." Pesquisa Agropecuária Brasileira 50, no. 12 (2015): 1225–31. http://dx.doi.org/10.1590/s0100-204x2015001200013.

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Resumo: O objetivo deste trabalho foi avaliar a reação de 48 acessos de tomateiro (Solanum lycopersicum), inclusive de espécies selvagens, a diferentes isolados das três raças de Fusarium oxysporum f. sp. lycopersici (FOL). Utilizaram-se marcadores moleculares ligados aos genes de resistência I-1, I-2 e I-3. A combinação de bioensaios e marcadores moleculares específicos mostrou elevada correlação para a maioria dos acessos. Acessos de S. peruvianum e S. corneliomuelleri apresentaram resistência contra todas as raças de FOL; a introgressão de fatores de resistência destes genótipos em germopla
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2

Rieseberg, L. H., C. R. Linder, and G. J. Seiler. "Chromosomal and genic barriers to introgression in Helianthus." Genetics 141, no. 3 (1995): 1163–71. http://dx.doi.org/10.1093/genetics/141.3.1163.

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Abstract The sexual transfer of genes between taxa possessing different structural karyotypes must involve the passage of genes through a chromosomal sterility barrier. Yet little is known about the effects of structural differences on gene introgression within or adjacent to the rearranged chromosomal fragments or about the patterns of introgression in collinear regions. Here, we employ 197 mapped molecular markers to study the effects of chromosomal structural differences on introgression in backcrossed progeny of the domesticated sunflower, Helianthus annuus, and its karyotypically divergen
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3

Garcia, G. M., H. T. Stalker, and G. Kochert. "Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers." Genome 38, no. 1 (1995): 166–76. http://dx.doi.org/10.1139/g95-021.

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Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea L. (2n = 4x = 40) and A. cardenasii Krapov. &W.C. Gregory (2n = 2x = 20) were analyzed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-specific bands, were used to evaluate the set of introgression lines. Thirty-four RFLP probes and 45 RAPD primers identified putative A. cardenasii introgressed chromosome segments in one or more lines. Introgressed segments were detected by RFLP analysis in 10 of the 11 linkage grou
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4

Wang, Jue, Chengkun Liu, Jie Chen, et al. "Genome-Wide Analysis Reveals Human-Mediated Introgression from Western Pigs to Indigenous Chinese Breeds." Genes 11, no. 3 (2020): 275. http://dx.doi.org/10.3390/genes11030275.

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Genetic variations introduced via introgression from Western to Chinese pigs have contributed to the performance of Chinese breeds in traits such as growth rate and feed conversion efficiency. However, little is known about the underlying genomic changes that occurred during introgression and the types of traits affected by introgression. To address these questions, 525 animals were characterized using an SNP array to detect genomic regions that had been introgressed from European to indigenous Chinese breeds. The functions of genes located in introgressed regions were also investigated. Our d
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5

Gouy, Alexandre, and Laurent Excoffier. "Polygenic Patterns of Adaptive Introgression in Modern Humans Are Mainly Shaped by Response to Pathogens." Molecular Biology and Evolution 37, no. 5 (2020): 1420–33. http://dx.doi.org/10.1093/molbev/msz306.

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Abstract Anatomically modern humans carry many introgressed variants from other hominins in their genomes. Some of them affect their phenotype and can thus be negatively or positively selected. Several individual genes have been proposed to be the subject of adaptive introgression, but the possibility of polygenic adaptive introgression has not been extensively investigated yet. In this study, we analyze archaic introgression maps with refined functional enrichment methods to find signals of polygenic adaptation of introgressed variants. We first apply a method to detect sets of connected gene
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6

Kitzberger, Cíntia Sorane Good, Maria Brígida dos Santos Scholz, Luiz Filipe Protasio Pereira, and Marta de Toledo Benassi. "Composição química de cafés árabica de cultivares tradicionais e modernas." Pesquisa Agropecuária Brasileira 48, no. 11 (2013): 1498–506. http://dx.doi.org/10.1590/s0100-204x2013001100011.

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O objetivo deste trabalho foi avaliar a influência da diversidade genética sobre a composição química de cultivares modernas e tradicionais de café arábica brasileiro. Cultivares tradicionais (Bourbon, Catuaí e Icatu) e modernas (Iapar 59, IPR 98, IPR 99 e IPR 103) foram cultivadas nas mesmas condições edafoclimáticas e submetidas a tratamentos pós-colheita padronizados. Determinaram-se os teores de sacarose, açúcares redutores, ácidos orgânicos (quínico, málico e cítrico), compostos fenólicos totais, ácido 5-cafeoilquínico, compostos nitrogenados (proteína, trigonelina e cafeína), lipídeos to
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7

Aragão, Fernando A. S., Cláudia S. da C. Ribeiro, Vicente Wagner D. Casali, and Leonardo de B. Giordano. "Cultivo de embriões de tomate in vitro visando a introgressão de genes de Lycopersicon peruvianum em L. esculentum." Horticultura Brasileira 20, no. 4 (2002): 605–10. http://dx.doi.org/10.1590/s0102-05362002000400019.

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Estudou-se diferentes protocolos de germinação e manutenção de híbridos interespecíficos (Lycopersicon esculentum X L. peruvianum) pela melhoria das condições de cultura in vitro bem como da aclimatação das plantas germinadas. Quanto à regeneração dos embriões, três formulações de meios de cultura para resgate de embrião foram avaliadas, em combinação com distintos períodos de tempo. A recuperação de híbridos interespecíficos foi influenciada pelo meio de cultura utilizado para o plaqueamento das sementes e pelo intervalo entre a polinização artificial e o plaqueamento. O meio HLH proporcionou
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8

Liu, Kevin J., Ethan Steinberg, Alexander Yozzo, Ying Song, Michael H. Kohn, and Luay Nakhleh. "Interspecific introgressive origin of genomic diversity in the house mouse." Proceedings of the National Academy of Sciences 112, no. 1 (2014): 196–201. http://dx.doi.org/10.1073/pnas.1406298111.

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We report on a genome-wide scan for introgression between the house mouse (Mus musculus domesticus) and the Algerian mouse (Mus spretus), using samples from the ranges of sympatry and allopatry in Africa and Europe. Our analysis reveals wide variability in introgression signatures along the genomes, as well as across the samples. We find that fewer than half of the autosomes in each genome harbor all detectable introgression, whereas the X chromosome has none. Further, European mice carry more M. spretus alleles than the sympatric African ones. Using the length distribution and sharing pattern
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9

Sillo, Fabiano, Matteo Garbelotto, Luana Giordano, and Paolo Gonthier. "Genic introgression from an invasive exotic fungal forest pathogen increases the establishment potential of a sibling native pathogen." NeoBiota 65 (May 28, 2021): 109–36. http://dx.doi.org/10.3897/neobiota.65.64031.

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Significant hybridization between the invasive North American fungal plant pathogen Heterobasidion irregulare and its Eurasian sister species H. annosum is ongoing in Italy. Whole genomes of nine natural hybrids were sequenced, assembled and compared with those of three genotypes each of the two parental species. Genetic relationships among hybrids and their level of admixture were determined. A multi-approach pipeline was used to assign introgressed genomic blocks to each of the two species. Alleles that introgressed from H. irregulare to H. annosum were associated with pathways putatively re
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10

Ramsay, L. D., D. E. Jennings, M. J. Kearsey, et al. "The construction of a substitution library of recombinant backcross lines in Brassica oleracea for the precision mapping of quantitative trait loci." Genome 39, no. 3 (1996): 558–67. http://dx.doi.org/10.1139/g96-071.

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The currently available methods for locating quantitative trait loci (QTLs) and measuring their effects in segregating populations lack precision unless individual QTLs have very high heritabilities. The use of recombinant backcross lines containing short regions of donor chromosome introgressed into a constant recipient background permits QTLs to be located with greater precision. The present paper describes the use of molecular markers to introgress defined short regions of chromosome from a donor doubled haploid calabrese line of Brassica oleracea (var. italica) into a recipient short gener
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11

Larkin, P. J., P. M. Banks, R. Bhati, et al. "From somatic variation to variant plants: mechanisms and applications." Genome 31, no. 2 (1989): 705–11. http://dx.doi.org/10.1139/g89-128.

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Plants from cell cultures show a high incidence of mutation. The causes of somaclonal variation are unknown, but the genetic consequences have been analysed. A range of genetic events are responsible, including single base changes; altered gene copy number; altered expression of multigene families; chromosome fragment interchanges; and mobilisation of transposable elements. Recent applications of these phenomena are discussed in this paper, for example, in vitro selection, somaclonal variation for agronomic traits, and limited gene transfer from cultured sexual and somatic hybrids. Research is
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12

Vanlerberghe, Flavie, Pierre Boursot, Josette Catalan, et al. "Analyse génétique de la zone d'hybridation entre les deux sous-espèces de souris Mus musculus domesticus et Mus musculus musculus en Bulgarie." Genome 30, no. 3 (1988): 427–37. http://dx.doi.org/10.1139/g88-072.

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The hybrid zone between the two subspecies of mice Mus musculus domesticus and Mus musculus musculus, which has been studied extensively in Denmark, crosses Europe to the Black Sea through the Alps and the Balkans. Two hundred and seventy-nine animals were captured in 22 localities along a transect across the Balkans. The animals were characterized for seven diagnostic nuclear loci by protein electrophoresis and by restriction pattern analysis of their mitochondrial DNA. The nuclear data show a sharp transition between the two subspecies, most of the variations in allele frequencies (from 0.9
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13

Ishii, T., D. S. Brar, D. S. Multani, and G. S. Khush. "Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa." Genome 37, no. 2 (1994): 217–21. http://dx.doi.org/10.1139/g94-030.

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Restriction fragment length polymorphism analysis was carried out to tag the alien genes for brown planthopper (BPH) resistance and earliness introgressed from wild species Oryza australiensis into cultivated rice, O. sativa L. One introgression line (IR65482-4-136-2-2), resistant to biotypes 1, 2, and 3 of BPH and early in flowering, was selected from BC2F4 of the cross between O. sativa (IR31917-45-3-2) and O. australiensis (accession 100882). Recurrent parent, O. australiensis, and introgression line were surveyed for RFLP using probes of chromosomes 10 and 12. Two probes, RG457 and CDO98,
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14

CARVALHO, GERALDO A., TUNEO SEDIYAMA, ANA LILIA ALZATE MARIN, EVERALDO G. BARROS, and MAURILIO A. MOREIRA. "Identificação de marcadores RAPD ligados a um gene de resistência ao cancro da haste da soja." Fitopatologia Brasileira 27, no. 5 (2002): 474–78. http://dx.doi.org/10.1590/s0100-41582002000500006.

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O cancro da haste da soja (Glycine max) é uma importante doença causada pelo fungo Diaporthe phaseolorum f. sp. meridionalis/Phomopsis phaseoli f. sp. meridionalis. Visando identificar marcadores RAPD associados a genes de resistência ao cancro da haste, causado pelo isolado CH8, presentes na linhagem UFV 91-61, foi realizado, inicialmente, um estudo sobre a herança da resistência, por meio do cruzamento desta linhagem com a variedade suscetível Paranaíba. Os resultados indicaram que um gene dominante controla a resistência a este isolado. Através de análises com marcadores moleculares na popu
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15

Fiorini, Cibelle VA, Derly José H. da Silva, Eduardo SG Mizubuti, et al. "Caracterização de linhagens de tomateiro originadas de cruzamento interespecífico quanto à resistência à requeima." Horticultura Brasileira 28, no. 2 (2010): 197–202. http://dx.doi.org/10.1590/s0102-05362010000200010.

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O trabalho teve por objetivo identificar linhagens de tomateiro resistentes à requeima. Foram avaliados 72 genótipos de tomateiro (70 linhagens F8 mais os acessos do BGH-UFV '184' e '1497'). As linhagens originaram-se de cruzamento interespecífico entre Solanum lycopersicum ('Santa Clara') e Solanum habrochaites f. glabratum (acesso BGH-UFV '6902'). Os experimentos foram conduzidos em campo, em delineamento experimental de blocos casualizados, utilizando-se três e duas repetições no primeiro e segundo experimento, respectivamente. As plantas foram inoculadas com uma suspensão de esporângios de
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16

Ji, Yuanfu, and Roger T. Chetelat. "GISH analysis of meiotic chromosome pairing in Solanum lycopersicoides introgression lines of cultivated tomato." Genome 50, no. 9 (2007): 825–33. http://dx.doi.org/10.1139/g07-069.

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Meiotic chromosome pairing was studied in introgression lines of cultivated tomato, Lycopersicon esculentum (= Solanum lycopersicum ), containing 1 or 2 chromosome segments from the wild species Solanum lycopersicoides . Genomic in situ hybridization (GISH) was used to compare the relative lengths at diakinesis of the different introgressed segments and to measure the chiasmate arm frequency for the chromosome pair involved in the introgression(s). Longer segments generally produced stronger GISH signals than shorter segments. GISH signal intensity also depended on whether or not an introgress
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17

Carrer Filho, Renato, Érico de Campos Dianese, and Marcos Gomes da Cunha. "Supressão da murcha de fusário em tomateiro por rizobactérias do gênero Bacillus1." Pesquisa Agropecuária Tropical 45, no. 3 (2015): 356–63. http://dx.doi.org/10.1590/1983-40632015v4535397.

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RESUMOMurchas vasculares em tomateiro constituem importante grupo de doenças incitadas por patógenos habitantes de solos, com destaque para Fusarium oxysporumf. sp. lycopersici, que ocasiona perdas significativas de produção. A introgressão de genes que expressam resistência é a principal medida de controle, mas, devido à rápida emergência de raças que suplantam a resistência, vem aumentando a importância do biocontrole, em integração com o controle genético. Objetivou-se avaliar a capacidade de isolados de rizobactérias, especialmente do gênero Bacillus, como agentes de biocontrole da murcha
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18

Rahman, L., M. S. Khanam, and H. J. Koh. "QTL analysis for yield related traits using populations derived from an indica-japonica hybrid in rice (Oryza sativaL.)." Czech Journal of Genetics and Plant Breeding 44, No. 3 (2008): 93–104. http://dx.doi.org/10.17221/16/2008-cjgpb.

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Introgression has been achieved from a wild species Oryza minuta (2n = 48, BBCC, Acc No.101141) into O. sativa subsp. indica IR71033-121-15. This introgression line was developed at International Rice Research Institute (IRRI) through embryo rescue as well as three backcrosses using IR31917-45-3-2 as a recurrent parent. These two IR lines resemble each other but differ in several important agronomic traits, which can be attributable to O. minuta introgressions. Out of 530 STS markers tested for introgression analysis, at least 14 introgressed chromosomal segments from O. minuta were detected t
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Suarez-Gonzalez, Adriana, Christian Lexer, and Quentin C. B. Cronk. "Adaptive introgression: a plant perspective." Biology Letters 14, no. 3 (2018): 20170688. http://dx.doi.org/10.1098/rsbl.2017.0688.

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Introgression is emerging as an important source of novel genetic variation, alongside standing variation and mutation. It is adaptive when such introgressed alleles are maintained by natural selection. Recently, there has been an explosion in the number of studies on adaptive introgression. In this review, we take a plant perspective centred on four lines of evidence: (i) introgression, (ii) selection, (iii) phenotype and (iv) fitness. While advances in genomics have contributed to our understanding of introgression and porous species boundaries (task 1), and the detection of signatures of se
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20

Flouri, Tomáš, Xiyun Jiao, Bruce Rannala, and Ziheng Yang. "A Bayesian Implementation of the Multispecies Coalescent Model with Introgression for Phylogenomic Analysis." Molecular Biology and Evolution 37, no. 4 (2019): 1211–23. http://dx.doi.org/10.1093/molbev/msz296.

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Abstract Recent analyses suggest that cross-species gene flow or introgression is common in nature, especially during species divergences. Genomic sequence data can be used to infer introgression events and to estimate the timing and intensity of introgression, providing an important means to advance our understanding of the role of gene flow in speciation. Here, we implement the multispecies-coalescent-with-introgression model, an extension of the multispecies-coalescent model to incorporate introgression, in our Bayesian Markov chain Monte Carlo program Bpp. The multispecies-coalescent-with-
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21

Smith, Joel, and Marcus R. Kronforst. "Do Heliconius butterfly species exchange mimicry alleles?" Biology Letters 9, no. 4 (2013): 20130503. http://dx.doi.org/10.1098/rsbl.2013.0503.

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Hybridization has the potential to transfer beneficial alleles across species boundaries, and there are a growing number of examples in which this has apparently occurred. Recent studies suggest that Heliconius butterflies have transferred wing pattern mimicry alleles between species via hybridization, but ancestral polymorphism could also produce a signature of shared ancestry around mimicry genes. To distinguish between these alternative hypotheses, we measured DNA sequence divergence around putatively introgressed mimicry loci and compared this with the rest of the genome. Our results revea
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22

Danilova, Tatiana V., Bernd Friebe, Bikram S. Gill, Jesse Poland, and Eric Jackson. "Chromosome Rearrangements Caused by Double Monosomy in Wheat-Barley Group-7 Substitution Lines." Cytogenetic and Genome Research 154, no. 1 (2018): 45–55. http://dx.doi.org/10.1159/000487183.

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Interspecific or introgressive hybridization is one of the driving forces in plant speciation, producing allopolyploids or diploids with rearranged genomes. The process of karyotype reshaping following homoploid interspecific hybridization has not been studied experimentally. Interspecific hybridization is widely used in plant breeding to increase genetic diversity and introgress new traits. Numerous introgression stocks were developed for hexaploid wheat Triticum aestivum L. (2n = 6x = 42, genome AABBDD). Double monosomic lines, containing one alien chromosome from the tertiary gene pool of w
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23

Rey, Olivier, Eve Toulza, Cristian Chaparro, et al. "Diverging patterns of introgression from Schistosoma bovis across S. haematobium African lineages." PLOS Pathogens 17, no. 2 (2021): e1009313. http://dx.doi.org/10.1371/journal.ppat.1009313.

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Hybridization is a fascinating evolutionary phenomenon that raises the question of how species maintain their integrity. Inter-species hybridization occurs between certain Schistosoma species that can cause important public health and veterinary issues. In particular hybrids between Schistosoma haematobium and S. bovis associated with humans and animals respectively are frequently identified in Africa. Recent genomic evidence indicates that some S. haematobium populations show signatures of genomic introgression from S. bovis. Here, we conducted a genomic comparative study and investigated the
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Visscher, Peter M., Chris S. Haley, and Robin Thompson. "Marker-Assisted Introgression in Backcross Breeding Programs." Genetics 144, no. 4 (1996): 1923–32. http://dx.doi.org/10.1093/genetics/144.4.1923.

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The efficiency of marker-assisted introgression in backcross populations derived from inbred lines was investigated by simulation. Background genotypes were simulated assuming that a genetic model of many genes of small effects in coupling phase explains the observed breed difference and variance in backcross populations. Markers were efficient in introgression backcross programs for simultaneously introgressing an allele and selecting for the desired genomic background. Using a marker spacing of 10–20 cM gave an advantage of one to two backcross generations selection relative to random or phe
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Chu, Y., R. Gill, J. Clevenger, P. Timper, C. C. Holbrook, and P. Ozias-Akins. "Identification of Rare Recombinants Leads to Tightly Linked Markers for Nematode Resistance in Peanut." Peanut Science 43, no. 2 (2016): 88–93. http://dx.doi.org/10.3146/ps16-12.1.

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ABSTRACT Strong host resistance to root-knot nematode (RKN; Meloidogyne arenaria) introgressed from a wild diploid species to cultivated peanut was previously shown to be located on a large chromosomal region of linkage group A09. Little to no recombination in mapping populations has hindered fine mapping of the resistance genes. In order to further delineate the introgressed region, additional polymorphic markers were added to the linkage group A09 using a recombinant inbred line population developed from Gregory x Tifguard in which Gregory is the susceptible parent and Tifguard is the resist
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Bosse, Mirte, Marcos S. Lopes, Ole Madsen, et al. "Artificial selection on introduced Asian haplotypes shaped the genetic architecture in European commercial pigs." Proceedings of the Royal Society B: Biological Sciences 282, no. 1821 (2015): 20152019. http://dx.doi.org/10.1098/rspb.2015.2019.

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Early pig farmers in Europe imported Asian pigs to cross with their local breeds in order to improve traits of commercial interest. Current genomics techniques enabled genome-wide identification of these Asian introgressed haplotypes in modern European pig breeds. We propose that the Asian variants are still present because they affect phenotypes that were important for ancient traditional, as well as recent, commercial pig breeding. Genome-wide introgression levels were only weakly correlated with gene content and recombination frequency. However, regions with an excess or absence of Asian ha
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27

Gonzalez-Segovia, Eric, Sergio Pérez-Limon, G. Carolina Cíntora-Martínez, et al. "Characterization of introgression from the teosinte Zea mays ssp. mexicana to Mexican highland maize." PeerJ 7 (May 3, 2019): e6815. http://dx.doi.org/10.7717/peerj.6815.

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Background The spread of maize cultivation to the highlands of central Mexico was accompanied by substantial introgression from the endemic wild teosinte Zea mays ssp. mexicana, prompting the hypothesis that the transfer of beneficial variation facilitated local adaptation. Methods We used whole-genome sequence data to map regions of Zea mays ssp. mexicana introgression in three Mexican highland maize individuals. We generated a genetic linkage map and performed Quantitative Trait Locus mapping in an F2 population derived from a cross between lowland and highland maize individuals. Results Int
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28

Valencia-Montoya, Wendy A., Samia Elfekih, Henry L. North, et al. "Adaptive Introgression across Semipermeable Species Boundaries between Local Helicoverpa zea and Invasive Helicoverpa armigera Moths." Molecular Biology and Evolution 37, no. 9 (2020): 2568–83. http://dx.doi.org/10.1093/molbev/msaa108.

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Abstract Hybridization between invasive and native species has raised global concern, given the dramatic increase in species range shifts and pest outbreaks due to anthropogenic dispersal. Nevertheless, secondary contact between sister lineages of local and invasive species provides a natural laboratory to understand the factors that determine introgression and the maintenance or loss of species barriers. Here, we characterize the early evolutionary outcomes following secondary contact between invasive Helicoverpa armigera and native H. zea in Brazil. We carried out whole-genome resequencing o
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Findley, Anthony S., Xinjun Zhang, Carly Boye, et al. "A signature of Neanderthal introgression on molecular mechanisms of environmental responses." PLOS Genetics 17, no. 9 (2021): e1009493. http://dx.doi.org/10.1371/journal.pgen.1009493.

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Ancient human migrations led to the settlement of population groups in varied environmental contexts worldwide. The extent to which adaptation to local environments has shaped human genetic diversity is a longstanding question in human evolution. Recent studies have suggested that introgression of archaic alleles in the genome of modern humans may have contributed to adaptation to environmental pressures such as pathogen exposure. Functional genomic studies have demonstrated that variation in gene expression across individuals and in response to environmental perturbations is a main mechanism
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Edelman, Nathaniel B., Paul B. Frandsen, Michael Miyagi, et al. "Genomic architecture and introgression shape a butterfly radiation." Science 366, no. 6465 (2019): 594–99. http://dx.doi.org/10.1126/science.aaw2090.

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We used 20 de novo genome assemblies to probe the speciation history and architecture of gene flow in rapidly radiating Heliconius butterflies. Our tests to distinguish incomplete lineage sorting from introgression indicate that gene flow has obscured several ancient phylogenetic relationships in this group over large swathes of the genome. Introgressed loci are underrepresented in low-recombination and gene-rich regions, consistent with the purging of foreign alleles more tightly linked to incompatibility loci. Here, we identify a hitherto unknown inversion that traps a color pattern switch l
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Santos, Carlos Antonio F., Daniela L. Leite, Nivaldo D. Costa, Valter R. Oliveira, Ierla Carla N. dos Santos, and Marciene A. Rodrigues. "Identificação dos citoplasmas "S", "T" e "N" via PCR em populações de cebola no Vale do São Francisco." Horticultura Brasileira 26, no. 3 (2008): 308–11. http://dx.doi.org/10.1590/s0102-05362008000300003.

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A identificação do tipo de citoplasma em cebola foi facilitada com a publicação de primers de DNA específicos para os tipos "S", "T" e "N". O objetivo do presente trabalho foi identificar, por meio de marcadores moleculares, o tipo de citoplasma presente na cultivar de cebola BRS São Francisco e numa população experimental em desenvolvimento na Embrapa Semi-Árido, de forma a orientar o desenvolvimento de híbridos de cebola adaptados à região. Na amostra de 19 plantas da 'BRS Alfa São Francisco' observou-se a amplificação de fragmentos de 180 pb, ausência do fragmento de 414 pb com os primers 5
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Perez, D. E., C. I. Wu, N. A. Johnson, and M. L. Wu. "Genetics of reproductive isolation in the Drosophila simulans clade: DNA marker-assisted mapping and characterization of a hybrid-male sterility gene, Odysseus (Ods)." Genetics 134, no. 1 (1993): 261–75. http://dx.doi.org/10.1093/genetics/134.1.261.

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Abstract In this study, we address the question of whether there exist major genes that cause complete male sterility in the interspecific hybrids of Drosophila and, if they do, how these genes may be characterized at the molecular level. Our approach is to introgress small segments of the X chromosome from Drosophila mauritiana (or Drosophila sechellia) into Drosophila simulans by repeated backcrosses for more than 20 generations. The introgressions are monitored by both visible mutations and a series of DNA markers. We compare the extent of introgressions that cause male sterility with those
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33

Nyine, Moses, Elina Adhikari, Marshall Clinesmith, Katherine W. Jordan, Allan K. Fritz, and Eduard Akhunov. "Genomic Patterns of Introgression in Interspecific Populations Created by Crossing Wheat with Its Wild Relative." G3: Genes|Genomes|Genetics 10, no. 10 (2020): 3651–61. http://dx.doi.org/10.1534/g3.120.401479.

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Introgression from wild relatives is a valuable source of novel allelic diversity for breeding. We investigated the genomic patterns of introgression from Aegilops tauschii, the diploid ancestor of the wheat D genome, into winter wheat (Triticum aestivum) cultivars. The population of 351 BC1F3:5 lines was selected based on phenology from crosses between six hexaploid wheat lines and 21 wheat-Ae. tauschii octoploids. SNP markers developed for this population and a diverse panel of 116 Ae. tauschii accessions by complexity-reduced genome sequencing were used to detect introgression based on the
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Miedaner, T., C. von der Ohe, V. Korzun, and E. Ebmeyer. "Introgression breeding - Effects and side effects of marker-based introduction of two non-adapted QTL for Fusarium head blight resistance into elite wheat." Plant Breeding and Seed Science 63, no. 1 (2011): 129–36. http://dx.doi.org/10.2478/v10129-011-0041-y.

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Introgression breeding - Effects and side effects of marker-based introduction of two non-adaptedQTLfor Fusarium head blight resistance into elite wheatFusarium head blight resistance (FHB) can be achieved by using improved adapted varieties as crossing partners or by a marker-assisted introgression of mapped QTL from non-adapted sources. In this long-term studyFhb1on chromosome 3BS andQfhs.ifa-5Alocated on chromosome 5A were introgressed into European elite spring and winter wheat to test effects on FHB resistance and side effects on agronomic performance andF. graminearumisolates and mixture
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Cullingham, Catherine I., Janice E. K. Cooke, and David W. Coltman. "Effects of introgression on the genetic population structure of two ecologically and economically important conifer species: lodgepole pine (Pinus contorta var. latifolia) and jack pine (Pinus banksiana)." Genome 56, no. 10 (2013): 577–85. http://dx.doi.org/10.1139/gen-2013-0071.

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Forest trees exhibit a remarkable range of adaptations to their environment, but as a result of frequent and long-distance gene flow, populations are often only weakly differentiated. Lodgepole and jack pine hybridize in western Canada, which adds the opportunity for introgression through hybridization to contribute to population structure and (or) adaptive variation. Access to large sample size, high density SNP datasets for these species would improve our ability to resolve population structure, parameterize introgression, and separate the influence of demography from adaptation. To accompli
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Suassuna, Taís de Moraes Falleiro, Adriana Regina Custódio, Kennedy Brunno de Brito Martins, Jair Heuert, Nelson Dias Suassuna, and Márcio de Carvalho Moretzsohn. "Uso de espécies silvestres como fontes de resistências às cercosporioses no amendoim." South American Sciences ISSN 2675-7222 1, no. 2 (2020): e2025. http://dx.doi.org/10.17648/sas.v1i2.25.

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A produtividade de amendoim pode ser reduzida devido a ocorrência de doenças foliares, necessitando, portanto, de controle químico com fungicidas para evitar perdas. Algumas espécies silvestres do gênero Arachis apresentam resistências às doenças foliares, sendo fontes de genes de resistências para introgressão no amendoim cultivado. O trabalho teve como objetivos avaliar a resistência às cercosporioses de progênies derivadas de espécies silvestres em combinação com linhagens e acompanhar os segmentos genômicos oriundos de espécies silvestres, possivelmente com genes de resistência às doenças
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Lourenção, André L., Walter J. Siqueira, Arlete M. T. Melo, Silvia R. L. Palazzo, Paulo C. T. Melo, and Addolorata Colariccio. "Resistência de cultivares e linhagens de tomateiro a Tomato chlorotic spot virus e a Potato virus Y." Fitopatologia Brasileira 30, no. 6 (2005): 609–14. http://dx.doi.org/10.1590/s0100-41582005000600007.

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Linhagens avançadas do programa de melhoramento do tomateiro (Lycopersicon esculentum) do IAC foram avaliadas em condições de campo em Campinas (SP) para resistência a tospovírus e a potyvírus, nos anos agrícolas 2002/2003 e 2003/2004, respectivamente. No primeiro ano, a única espécie de tospovírus que ocorreu na área experimental foi Tomato chlorotic spot virus (TCSV). As sete linhagens do grupo IAC exibiram baixa porcentagem de plantas sintomáticas em duas avaliações, com médias abaixo de 28%; as cultivares testadas mostraram-se altamente suscetíveis, com médias acima de 85%, à exceção de 'F
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38

Wolfe, Marnin D., Guillaume J. Bauchet, Ariel W. Chan, et al. "Historical Introgressions from a Wild Relative of Modern Cassava Improved Important Traits and May Be Under Balancing Selection." Genetics 213, no. 4 (2019): 1237–53. http://dx.doi.org/10.1534/genetics.119.302757.

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Introgression of alleles from wild relatives has often been adaptive in plant breeding. However, the significance of historical hybridization events in modern breeding is often not clear. Cassava (Manihot esculenta) is among the most important staple foods in the world, sustaining hundreds of millions of people in the tropics, especially in sub-Saharan Africa. Widespread genotyping makes cassava a model for clonally propagated root and tuber crops in the developing world, and provides an opportunity to study the modern benefits and consequences of historical introgression. We detected large in
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Cong, Qian, Jinhui Shen, Dominika Borek, et al. "When COI barcodes deceive: complete genomes reveal introgression in hairstreaks." Proceedings of the Royal Society B: Biological Sciences 284, no. 1848 (2017): 20161735. http://dx.doi.org/10.1098/rspb.2016.1735.

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Two species of hairstreak butterflies from the genus Calycopis are known in the United States: C. cecrops and C. isobeon . Analysis of mitochondrial COI barcodes of Calycopis revealed cecrops -like specimens from the eastern US with atypical barcodes that were 2.6% different from either USA species, but similar to Central American Calycopis species. To address the possibility that the specimens with atypical barcodes represent an undescribed cryptic species, we sequenced complete genomes of 27 Calycopis specimens of four species: C. cecrops , C. isobeon , C. quintana and C. bactra . Some of th
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Rangel, Priscila Nascimento, Rosana Pereira Vianello, Arthur Tavares Oliveira Melo, Paulo Hideo Nakano Rangel, João Antônio Mendonça, and Claudio Brondani. "Yield QTL analysis of Oryza sativa x O. glumaepatula introgression lines." Pesquisa Agropecuária Brasileira 48, no. 3 (2013): 280–86. http://dx.doi.org/10.1590/s0100-204x2013000300006.

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The objective of this work was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between Oryza sativa and O. glumaepatula, and to identify the SSR markers associated to yield. The wild accession RS‑16 (O. glumaepatula) was used as donor parent in the backcross with the high yielding cultivar Cica‑8 (O. sativa). A set of 114 BC2F1 introgression lines was genotyped with 141 polymorphic SSR loci distributed across the whole rice genome. Molecular analysis showed that in average 22% of the O. glumaepatula genome was
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41

Forsythe, Evan S., Andrew D. L. Nelson, and Mark A. Beilstein. "Biased Gene Retention in the Face of Introgression Obscures Species Relationships." Genome Biology and Evolution 12, no. 9 (2020): 1646–63. http://dx.doi.org/10.1093/gbe/evaa149.

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Abstract Phylogenomic analyses are recovering previously hidden histories of hybridization, revealing the genomic consequences of these events on the architecture of extant genomes. We applied phylogenomic techniques and several complementary statistical tests to show that introgressive hybridization appears to have occurred between close relatives of Arabidopsis, resulting in cytonuclear discordance and impacting our understanding of species relationships in the group. The composition of introgressed and retained genes indicates that selection against incompatible cytonuclear and nuclear–nucl
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42

Hsieh, PingHsun, Mitchell R. Vollger, Vy Dang, et al. "Adaptive archaic introgression of copy number variants and the discovery of previously unknown human genes." Science 366, no. 6463 (2019): eaax2083. http://dx.doi.org/10.1126/science.aax2083.

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Copy number variants (CNVs) are subject to stronger selective pressure than single-nucleotide variants, but their roles in archaic introgression and adaptation have not been systematically investigated. We show that stratified CNVs are significantly associated with signatures of positive selection in Melanesians and provide evidence for adaptive introgression of large CNVs at chromosomes 16p11.2 and 8p21.3 from Denisovans and Neanderthals, respectively. Using long-read sequence data, we reconstruct the structure and complex evolutionary history of these polymorphisms and show that both encode
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Herrera, Juan C., Marie C. Combes, Hernando Cortina, and Philippe Lashermes. "Factors influencing gene introgression into the allotetraploid Coffea arabica L. from its diploid relatives." Genome 47, no. 6 (2004): 1053–60. http://dx.doi.org/10.1139/g04-048.

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Factors controlling gene introgression into cultivated arabica coffee (Coffea arabica L.) were investigated. Interspecific triploid hybrid plants between the tetraploid species C. arabica (2n = 44) and a diploid species (2n = 22), either Coffea canephora or Coffea eugenioides, were backcrossed to C. arabica (male parent). Flow cytometric analysis of the nuclear DNA content revealed that most of the BC1 individuals derived from triploid hybrids involving C. eugenioides were tetraploid or nearly tetraploid. Among the gametes produced by the interspecific triploid hybrids, those possessing approx
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Bowman, Jeff, Kaela Beauclerc, A. Hossain Farid, Heather Fenton, Cornelya F. C. Klütsch, and Albrecht I. Schulte-Hostedde. "Hybridization of domestic mink with wild American mink (Neovison vison) in eastern Canada." Canadian Journal of Zoology 95, no. 6 (2017): 443–51. http://dx.doi.org/10.1139/cjz-2016-0171.

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Farmed American mink (Neovison vison (Schreber, 1777)) pose a risk to biodiversity owing to escape and release from farms. Feral mink may affect native species in locations where American mink are not endemic, such as Europe. In contrast, escaping domestic mink may hybridize with wild mink in North America, leading to introgression of domestic traits via hybrid-mediated gene flow. We tested this idea in eastern Canada, which has a history of mink farming. We sampled known domestic and free-ranging mink, and profiled 508 individuals at 15 microsatellite loci. We found that 33% of free-ranging m
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45

Lyman, Richard F., and Trudy F. C. Mackay. "Candidate Quantitative Trait Loci and Naturally Occurring Phenotypic Variation for Bristle Number in Drosophila melanogaster: The Delta-Hairless Gene Region." Genetics 149, no. 2 (1998): 983–98. http://dx.doi.org/10.1093/genetics/149.2.983.

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AbstractDelta (Dl) and Hairless (H) are two chromosome 3 candidate neurogenic loci that might contribute to naturally occurring quantitative variation for sensory bristle number. To evaluate this hypothesis, we assessed quantitative genetic variation in abdominal and sternopleural bristle numbers among homozygous isogenic third chromosomes sampled from nature and substituted into the Samarkand (Sam) inbred chromosome 1 and 2 background; among homozygous lines in which the wild-derived Dl-H gene region was introgressed into the Sam chromosome 3 background; and among Dl-H region introgression li
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46

García, Beatriz A., Adalgisa Caccone, Kostas D. Mathiopoulos, and Jeffrey R. Powell. "Inversion Monophyly in African Anopheline Malaria Vectors." Genetics 143, no. 3 (1996): 1313–20. http://dx.doi.org/10.1093/genetics/143.3.1313.

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Abstract The African Anopheles gambzae complex of six sibling species has many polymorphic and fixed paracentric inversions detectable in polytene chromosomes. These have been used to infer phylogenetic relationships as classically done with Drosophila. Two species, A. gambiae and A. merus, were thought to be sister taxa based on a shared X inversion designated Xag. Recent DNA data have conflicted with this phylogenetic inference as they have supported a sister taxa relationship of A. gambiaeand A. arabiensis. A possible explanation is that the Xag is not monophyletic. Here we present data fro
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47

Zhang, Xinjun, Kelsey E. Witt, Mayra M. Bañuelos, et al. "The history and evolution of the Denisovan-EPAS1 haplotype in Tibetans." Proceedings of the National Academy of Sciences 118, no. 22 (2021): e2020803118. http://dx.doi.org/10.1073/pnas.2020803118.

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Recent studies suggest that admixture with archaic hominins played an important role in facilitating biological adaptations to new environments. For example, interbreeding with Denisovans facilitated the adaptation to high-altitude environments on the Tibetan Plateau. Specifically, the EPAS1 gene, a transcription factor that regulates the response to hypoxia, exhibits strong signatures of both positive selection and introgression from Denisovans in Tibetan individuals. Interestingly, despite being geographically closer to the Denisova Cave, East Asian populations do not harbor as much Denisova
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Musundire, L., J. Derera, S. Dari, and P. Tongoona. "Genetic Variation and Path Coefficient Analysis of Introgressed Maize Inbred Lines for Economic Traits." Journal of Agricultural Science 11, no. 17 (2019): 135. http://dx.doi.org/10.5539/jas.v11n17p135.

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Knowledge of the effects of introgressing temperate maize germplasm in tropical elite inbred lines on genetic variation and relationship between grain yield and its components is limited. In this study, the objective was to evaluate introgressed maize inbred lines for selected economic traits. Field evaluation was carried out on 122 inbred lines comprising sets of introgressed lines from three selection environments, parental inbred lines and two common checks. Genetic variation was significant (P < 0.05) for all the major economic traits among inbred lines within and across sets. Herit
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Oziolor, Elias M., Noah M. Reid, Sivan Yair, et al. "Adaptive introgression enables evolutionary rescue from extreme environmental pollution." Science 364, no. 6439 (2019): 455–57. http://dx.doi.org/10.1126/science.aav4155.

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Radical environmental change that provokes population decline can impose constraints on the sources of genetic variation that may enable evolutionary rescue. Adaptive toxicant resistance has rapidly evolved in Gulf killifish (Fundulus grandis) that occupy polluted habitats. We show that resistance scales with pollution level and negatively correlates with inducibility of aryl hydrocarbon receptor (AHR) signaling. Loci with the strongest signatures of recent selection harbor genes regulating AHR signaling. Two of these loci introgressed recently (18 to 34 generations ago) from Atlantic killifis
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Hill, Geoffrey E. "Reconciling the Mitonuclear Compatibility Species Concept with Rampant Mitochondrial Introgression." Integrative and Comparative Biology 59, no. 4 (2019): 912–24. http://dx.doi.org/10.1093/icb/icz019.

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Abstract The mitonuclear compatibility species concept defines a species as a population that is genetically isolated from other populations by uniquely coadapted mitochondrial (mt) and nuclear genes. A key prediction of this hypothesis is that the mt genotype of each species will be functionally distinct and that introgression of mt genomes will be prevented by mitonuclear incompatibilities that arise when heterospecific mt and nuclear genes attempt to cofunction to enable aerobic respiration. It has been proposed, therefore, that the observation of rampant introgression of mt genotypes from
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