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Journal articles on the topic 'Ionic detergents'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Cham, B. E., P. Roeser, and A. Nikles. "Effects of non-ionic and anionic detergents on lipid-associated tissue ferritin." Clinical Chemistry 34, no. 1 (1988): 152–54. http://dx.doi.org/10.1093/clinchem/34.1.152.

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Abstract Lipid-associated ferritin from homogenates of guinea pig liver is released from its conjugate(s) by incubation with the non-ionic detergents Triton X-100 and Nonidet P-40 but not by incubation with the anionic detergent deoxycholate. The amount of lipid-associated ferritin released from its conjugate(s) depends on the concentration of the non-ionic detergents. At a final non-ionic detergent concentration of about 20 g/L, all lipid-associated ferritin is released from its conjugate(s) in a liver homogenate. The amount released is identical with the amount of the lipid-associated ferrit

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Kurisu, Toshikatsu, Hiroyasu Taguchi, Bela Paal, Nan Yang та Wilfred L. F. Armarego. "Glyceryl-ether Monooxygenase [EC 1.14.16.5J Part VII Effects of Alkyl Phosphocholines and Acyl L-α-lyso-Phosphatidylcholines Detergents on Enzyme Activity". Pteridines 5, № 3 (1994): 95–101. http://dx.doi.org/10.1515/pteridines.1994.5.3.95.

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Summary The effects of the ionic detergents dodecyl, tetradecyl, hexadecyl and octadecyl phosphocholines, and dodecanoyl, tetradecanoyl, hexadecanoyl and octadecanoyl L-a-lyso-phosphatidylcholines on the enzyme activity of glyceryl-ether monooxygenase are reported. All these detergents inhibit the monooxygenase at concentrations above a threshold value which is above the relative critical micelle concentrations implying that the micelles interact passively with the microsomal membrane below the threshold concentration. The kinetics of inhibition by the C14 and C16 detergents above the threshol

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Lee, Joshua Y., Katherine M. Selfridge, Eric M. Kohn, Timothy D. Vaden, and Gregory A. Caputo. "Effects of Ionic Liquid Alkyl Chain Length on Denaturation of Myoglobin by Anionic, Cationic, and Zwitterionic Detergents." Biomolecules 9, no. 7 (2019): 264. http://dx.doi.org/10.3390/biom9070264.

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The unique electrochemical properties of ionic liquids (ILs) have motivated their use as solvents for organic synthesis and green energy applications. More recently, their potential in pharmaceutical chemistry has prompted investigation into their effects on biomolecules. There is evidence that some ILs can destabilize proteins via a detergent-like manner; however, the mechanism still remains unknown. Our hypothesis is that if ILs are denaturing proteins via a detergent-like mechanism, detergent-mediated protein unfolding should be enhanced in the presence of ILs. The properties of myoglobin w

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Claffey, David J., Jeffrey D. Meyer, Robert Beauvais, et al. "Long chain arginine esters: A new class of cationic detergents for preparation of hydrophobic ion-paired complexes." Biochemistry and Cell Biology 78, no. 1 (2000): 59–65. http://dx.doi.org/10.1139/o99-072.

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The ability of stoichiometric amounts (based on charged groups) of ionic detergents to bind to oppositely charged ionic compounds has been recently reviewed. These hydrophobic ion-paired (HIP) complexes display altered solubility properties. Most of the work to date on HIP compelxes has focused on basic drugs and anionic detergents. It would be extremely useful to extend this approach to acidic compounds, including DNA and RNA. However, most cationic detergents are relatively toxic. It is hypothesized that detergents constructed from naturally occurring or well tolerated components, coupled by

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5

SOLOMON, Keith R., Mark A. MALLORY, and Robert W. FINBERG. "Determination of the non-ionic detergent insolubility and phosphoprotein associations of glycosylphosphatidylinositol-anchored proteins expressed on T cells." Biochemical Journal 334, no. 2 (1998): 325–33. http://dx.doi.org/10.1042/bj3340325.

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Glycosylphosphatidylinositol (GPI)-anchored proteins are poorly solublized in non-ionic detergents such as Triton X-100 and Nonidet P40, but are easily solublized by detergents with high critical micelle concentrations such as octylglucoside. This solubility profile has been suggested to be due to the localization of GPI-anchored proteins to lipid microdomains rich in cholesterol and sphingolipids. Additionally, GPI-anchored proteins expressed on haemopoietic cells have been shown to associate with src-family tyrosine kinases and heterotrimeric G proteins. Despite these observations, the non-i

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Ismail, Alnashir A., and Roger Hand. "Membrane D2 T antigen: characterization and comparison with its nuclear counterpart." Biochemistry and Cell Biology 66, no. 8 (1988): 928–34. http://dx.doi.org/10.1139/o88-106.

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Up to 40% of membrane T antigen (D2 T antigen) produced by the adenovirus 2–simian virus 40 hybrid, Ad2+D2, remained tightly associated with membranes under alkaline conditions up to pH 11.5. The antigen could not be totally solubilized by treatment with ionic detergents, nonionic detergents, or both. These properties are characteristic of integral membrane proteins. Sephacryl S-300 chromatography in high salt in the presence of Brij-99 showed that the nuclear form of D2 T antigen was dissociated to low molecular weight species, while the membrane form eluted as a complex of high molecular wei

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7

Brinkworth, Craig S., and David J. Bourne. "Selected Non-Ionic Biological Detergents Enhance Signal Intensity of Intact Bovine Serum Albumin by Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry." European Journal of Mass Spectrometry 13, no. 5 (2007): 311–19. http://dx.doi.org/10.1255/ejms.886.

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Recently, we showed that the signal intensity of intact protein by matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry measurement can be enhanced at least an order of magnitude by the addition of Tween80 to the analyte solution. We did not ascertain whether this effect was limited to Tween80 or if it was more universal of biological detergents. This paper discusses our investigations into this question. A variety of chemically diverse detergents were added to analyte solutions containing bovine serum albumin (BSA) to determine whether there was significant signal enhancement

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8

Yakovlev, A. A., T. A. Druzhkova, A. B. Guekht, and N. V. Gulyaeva. "Sensitivity of Extracellular Vesicles from Human Blood Serum to Various Detergents." Biomedical Chemistry: Research and Methods 3, no. 4 (2020): e00143. http://dx.doi.org/10.18097/bmcrm00143.

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Blood exosomes and microvesicles, collectively known as small extracellular vesicles (sEV), are vesicles about 100-150 nm in size. Small EV are involved in various aspects of signaling in the body; in addition, they can serve as markers of various pathologies. For biochemical studies, vesicle solubilization is often required. We tested the ability of various detergents to dissolve membranes of the sEV. Small EV were isolated from the blood serum of healthy volunteers by gel filtration on Sepharose CL-2B and tried to solubilize them using the anionic detergent DOC (sodium deoxycholate), non-ion

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9

Amtmann, Eberhard, Karin Müller-Decker, Angela Hoss, Gunnar Schalasta, Clemens Doppler, and Gerhard Sauer. "Synergistic antiviral effect of xanthates and ionic detergents." Biochemical Pharmacology 36, no. 9 (1987): 1545–49. http://dx.doi.org/10.1016/0006-2952(87)90124-9.

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10

Shogomori, H., and D. A. Brown. "Use of Detergents to Study Membrane Rafts: The Good, the Bad, and the Ugly." Biological Chemistry 384, no. 9 (2003): 1259–63. http://dx.doi.org/10.1515/bc.2003.139.

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Abstract Eukaryotic cell membranes contain microdomains called lipid rafts, which are cholesterol-rich domains in which lipid acyl chains are tightly packed and highly extended. A variety of proteins associate preferentially with rafts, and this raft association is important in a wide range of functions. A powerful and widely used method for studying lipid rafts takes advantage of their insolubility in non-ionic detergents. Here we describe the basis of detergent insolubility, and review strengths, limitations, and unresolved puzzles regarding this method.

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RADEVA, Galina, and Frances J. SHAROM. "Isolation and characterization of lipid rafts with different properties from RBL-2H3 (rat basophilic leukaemia) cells." Biochemical Journal 380, no. 1 (2004): 219–30. http://dx.doi.org/10.1042/bj20031348.

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Lipid rafts are plasma-membrane microdomains that are enriched in certain lipids (sphingolipids, glycosphingolipids and cholesterol), as well as in lipid-modified proteins. Rafts appear to exist in the liquid-ordered phase, which contributes to their partitioning from the surrounding liquid-disordered glycerophospholipid environment. DRM (detergent-resistant membrane) fractions isolated from cells are believed to represent coalesced lipid rafts. We have employed extraction using two different non-ionic detergents, Brij-96 and Triton X-100, to isolate detergent-resistant lipid rafts from rat ba

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12

Vanden Bossche, Geert. "Use of ionic detergents for enterovirus recovery from waste water." Microbiological Research 149, no. 3 (1994): 231–40. http://dx.doi.org/10.1016/s0944-5013(11)80063-7.

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13

Samson, Michel, Jeannine Osty, Hélène Thibout, and Jean-Paul Blondeau. "Solubilization, reconstitution and molecular properties of the triiodothyronine transport protein from rat erythrocyte membranes." European Journal of Endocrinology 134, no. 5 (1996): 660–68. http://dx.doi.org/10.1530/eje.0.1340660.

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Samson M, Osty J, Thibout H, Blondeau J-P. Solubilization, reconstitution and molecular properties of the triiodothyronine transport protein from rat erythrocyte membranes. Eur J Endocrinol 1996;134: 660–8. ISSN 0804–4643 Triiodothyronine (T3) transport through the mammalian erythrocyte membrane is mediated by a transport system related to the aromatic amino acid transport system T. The T3-binding component of this transport system could be photolabeled with [125I]T3 as a 52-kD protein, and subsequently solubilized with non-ionic detergents. Upon purification by ion-exchange chromatography, th

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Holy, J. M., and J. A. Oaks. "Cytoskeletal features of epithelia prepared by “extractive fixation“." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 902–3. http://dx.doi.org/10.1017/s0424820100128791.

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Techniques such as whole mount (high-voltage) electron microscopy of cultured cells (frequently extracted with non-ionic detergents), rapid-freezing (followed either by freeze-substitution or deep etching and rotary-replication), and reversible embedment of tissue have furnished novel insights into cytoskeletal architecture. Another approach in the examination of cytoskeletal morphology has been the modification of routine fixation and thin-section methods. Minimizing osmium exposure and including tannic acid in the primary fixative has been found to improve cytoskeletal preservation. Maupin a

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15

Bing, R. J., A. Termin, A. Conforto, R. Dudek, and M. J. Hoffmann. "Membrane function and vascular reactivity." Bioscience Reports 13, no. 2 (1993): 61–67. http://dx.doi.org/10.1007/bf01145958.

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This communication examines the possibility that nitric oxide (NO) production by endothelial cells results from changes in cell membrane fluidity. Lysophosphatidylcholine (LPC) alters fluidity of the endothelial cell membranes causing vascular relaxation. Through membrane alterations LPC influences function of a number of membrane receptors and modulates enzyme activity. As a result of detergent action, lysophosphatidylcholine (LPC) causes activation of guanylate cyclase, stimulates syalytransferase and regulates protein kinase C activity. It has already been demonstrated that ionic detergents

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16

Babiychuk, Eduard B., and Annette Draeger. "Biochemical characterization of detergent-resistant membranes: a systematic approach." Biochemical Journal 397, no. 3 (2006): 407–16. http://dx.doi.org/10.1042/bj20060056.

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Lateral segregation of cholesterol- and sphingomyelin-rich rafts and glycerophospholipid-containing non-raft microdomains has been proposed to play a role in a variety of biological processes. The most compelling evidence for membrane segregation is based on the observation that extraction with non-ionic detergents leads to solubilization of a subset of membrane components only. However, one decade later, a large body of inconsistent detergent-extraction data is threatening the very concept of membrane segregation. We have assessed the validity of the existing paradigms and we show the followi

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17

Prokofyeva, G. M., and D. A. Pisanenko. "RESEARCH OF ENVIRONMENTALLY CLEAN TECHNICAL DETERGENTS." Energy Technologies & Resource Saving, no. 1 (April 24, 2018): 43–47. http://dx.doi.org/10.33070/etars.1.2018.06.

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Problematic places in the modern economy is reckless depletion of natural resources, raw materials and fuels, excessive using of energy and environmental issues. Solving these issues in the gas and chemical industry achieved by preventing formation of the deposits on the compressor’s elements, and also implementation of the non-waste and low-waste productions and alternative energy sources. The deposits on the internal elements of the gas-air channels results in a significant loss of power, increases energy costs, reduces operational reliability of plants, through the activation of corrosion a

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18

Egelund, R., S. Jensen, K. W. Rodenburg, and P. A. Andreasen. "Solvent Effects on Activity and Conformation of Plasminogen Activator Inhibitor-1." Thrombosis and Haemostasis 81, no. 03 (1999): 407–14. http://dx.doi.org/10.1055/s-0037-1614487.

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SummaryWe have studied effects of the solvent composition on the activity and the conformation of human plasminogen activator inhibitor-1 (PAI-1) from HT-1080 fibrosarcoma cells. Non-ionic detergents, including Triton X-100, reduced the inhibitory activity of PAI-1 more than 20-fold at 0° C, but less than 2-fold at 37° C, while glycerol partly prevented the detergent-induced activity-loss at 0° C. The activity-loss was associated with an increase in PAI-1 substrate behaviour. Evaluating the PAI-1 conformation by proteolytic susceptibility of specific peptide bonds, we found that the V8-protein

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19

Roy, Reena. "Haptoglobin typing from fatty tissues after extraction with non-ionic detergents." Forensic Science International 49, no. 1 (1991): 95–102. http://dx.doi.org/10.1016/0379-0738(91)90176-j.

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20

Rajakumari, Sona, Malathi Srinivasan, and Ram Rajasekharan. "Spectrophotometric method for quantitative determination of nonionic, ionic and zwitterionic detergents." Journal of Biochemical and Biophysical Methods 68, no. 2 (2006): 133–37. http://dx.doi.org/10.1016/j.jbbm.2006.04.004.

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21

Saeidian, Shahriar, Ezzatollah Keyhani, and Jacqueline Keyhani. "Effect of Ionic Detergents, Nonionic Detergents, and Chaotropic Agents on Polyphenol Oxidase Activity from Dormant Saffron (Crocus sativusL.) Corms." Journal of Agricultural and Food Chemistry 55, no. 9 (2007): 3713–19. http://dx.doi.org/10.1021/jf063749n.

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22

CARDENAS, MARIA E., THIERRY LAROCHE, and SUSAN M. GASSER. "The composition and morphology of yeast nuclear scaffolds." Journal of Cell Science 96, no. 3 (1990): 439–50. http://dx.doi.org/10.1242/jcs.96.3.439.

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The yeast nuclear scaffold has been shown to bind with high affinity to genomic sequences that permit autonomous DNA replication of plasmids (ARS elements). We describe here conditions for the isolation of a histone-free nuclear substructure, the nuclear scaffold, from Saccharomyces cerevisiae. We examine the protein composition of this structure, and the conditions under which topoisomerase II, the nuclear factor RAP-1 and RNA polymerase II cofractionate with the scaffold. We find that exposure of nuclei to a combined metal and heat treatment (0.5mH Cu2+, 37°C) prior to detergent extraction i

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23

Schmidt, A., J. Gottstein, H. Scheer, and A. Scherz. "Short-Wavelength Absorbing Complexes of Chlorophyll a in Micellar Solution of Cationic Detergents." Zeitschrift für Naturforschung C 45, no. 6 (1990): 729–32. http://dx.doi.org/10.1515/znc-1990-0627.

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Abstract Micellar aggregates have been prepared from chlorophyll a. Whereas most detergents including non-ionic, zwitterionic and anionic ones, produce aggregates with long-wavelength shifted and enhanced QY absorption, the cationic detergents hexadecyl-trimethylammonium-bromide and hexadecyl-pyridiniumchloride produce complexes with opposite traits: The QY absorption is shifted to shorter wavelengths, and it is hypochromic. The optical activity is increased as in the long-wave length shifted complexes, but the cd bands are non-conservative and coincide with the absorption maxima. The comple

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Izsvák, Zsuzsa, Zsolt Jobbágy, and Ernö Duda. "Purification And Characterization Of Ceql Restriction Endonuclease." Zeitschrift für Naturforschung C 47, no. 11-12 (1992): 830–34. http://dx.doi.org/10.1515/znc-1992-11-1208.

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Ceql, a type II restriction endonuclease, an isoschizomer of EcoRV was purified to apparent homogeneity by a combination of salt precipitation, ion exchange, dye affinity and hydrophobic interaction chromatographies. The crude enzyme was present in the form of large aggregates that could be pelleted by high speed centrifugation. The enzyme was not associated with cellular membranes, though non-ionic detergents lowered the apparent size of the aggregates. The purified enzyme also showed a tendency to form large molecular mass (66-600 kDa) complexes under physiological conditions, in the absence

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DeNofrio, D., T. C. Hoock, and I. M. Herman. "Functional sorting of actin isoforms in microvascular pericytes." Journal of Cell Biology 109, no. 1 (1989): 191–202. http://dx.doi.org/10.1083/jcb.109.1.191.

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We characterized the form and distribution of muscle and nonmuscle actin within retinal pericytes. Antibodies with demonstrable specificities for the actin isoforms were used in localization and immunoprecipitation experiments to identify those cellular domains that were enriched or deficient in one or several actin isoforms. Living pericyte behavior was monitored with phase-contract video microscopy before fixation to identify those cellular areas that might preferentially be stained with either of the fluorescent antiactins or phallotoxins. Antibody and phallotoxin staining of pericytes reve

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Bogen, Oliver, Petar Milosevic, Hans Daroszi, Mathias Dreger, Stefan Hecht, and Ferdinand Hucho. "Light-triggered conversion of non-ionic into ionic surfactants: towards chameleon detergents for 2-D gel electrophoresis." Photochemical & Photobiological Sciences 11, no. 3 (2012): 497. http://dx.doi.org/10.1039/c1pp05316g.

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27

Fürhacker, M., and R. Haberl. "Composting of sewage sludge in a rotating vessel." Water Science and Technology 32, no. 11 (1995): 121–25. http://dx.doi.org/10.2166/wst.1995.0418.

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Composting of sewage sludge can enhance its quality and suitability for agricultural use. In this work the optimal conditions for composting sewage sludge of small domestic wastewater treatment plants in a rotating vessel with the aim of sanitary safety were investigated. An intensive control programme with regard to viruses, bacteria and parasites was carried out that showed that the hygienic quality was satisfactory. A second aspect was the investigation of a possible reduction of hazardous organic and inorganic pollutants. The amount of inorganic micropollutants was low and did not change b

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Lezin, George, Michael R. Kuehn, and Luca Brunelli. "Hofmeister series salts enhance purification of plasmid DNA by non-ionic detergents." Biotechnology and Bioengineering 108, no. 8 (2011): 1872–82. http://dx.doi.org/10.1002/bit.23116.

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Mitru, Daniel, Gheorghe Nechifor, Stefania Gheorghe, et al. "Removal and Effects of Surfactants in Activated Sludge System." Revista de Chimie 71, no. 1 (2020): 100–106. http://dx.doi.org/10.37358/rc.20.1.7819.

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The widespread use of detergents has raised concern with regard to the environmental pollution caused by their active substances, which are biorefractory, toxic or persistent. Even though anionic and non-ionic surfactants often used in commercial detergents composition are reasonably degradable under aerobic conditions and not particularly toxic at low concentrations, high concentration of surfactants influenced the activated sludge activity and are harmful for the aquatic environment. In the literature, data on the biodegradability and/or ecotoxicity of a certain type of surfactant (anionic,

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Nicholls, P., and S. Shaughnessy. "Effects of detergents and cytochrome c binding on scalar and vectorial proton ejection by proteoliposomes containing cytochrome oxidase." Biochemical Journal 228, no. 1 (1985): 201–10. http://dx.doi.org/10.1042/bj2280201.

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The detergent lauryl maltoside abolishes respiratory control and proton ejection by cytochrome c oxidase-containing proteoliposomes over a narrow concentration range. Expression of cryptic activity (inward-facing oxidase) is released over the same concentration range. Catalytic functions (Vmax. and Km) of the enzyme are not changed by the detergent. Lipid micelles containing detergent bind approximately the same amount of cytochrome c as do vesicles containing an equivalent amount of lipid. Uncoupler-insensitive proton release is seen when proteoliposomes are pulsed with ferrocytochrome c at l

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Nishiya, Y., M. Yamashita, Y. Murooka, I. Fujii, and N. Hirayama. "Effect of non-ionic detergents on apparent enzyme mechanism: V121A mutant of Streptomyces cholesterol oxidase endowed with enhanced sensitivity towards detergents." Protein Engineering Design and Selection 11, no. 8 (1998): 609–11. http://dx.doi.org/10.1093/protein/11.8.609.

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Adir, Noam. "Crystallization of the oxygen-evolving reaction centre of photosystem II in nine different detergent mixtures." Acta Crystallographica Section D Biological Crystallography 55, no. 4 (1999): 891–94. http://dx.doi.org/10.1107/s0907444998016497.

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Oxygen-evolving photosystem II reaction centres (RCII) isolated from both spinach and pea have been crystallized. A single crystal form grew from RCII monomers in the presence of nine different three-component mixtures of non-ionic detergents and heptane-1,2,3-triol. The crystals grew as hexagonal rods with dimensions of up to 1 × 0.3 × 0.3 mm. The crystals diffracted to a maximum resolution of 6.5 Å and belong to a hexagonal space group with unit-cell parameters a = 495, b = 495, c = 115 Å, α = β = 90, γ = 120°. The growth of a single crystal form in the presence of such a large variety of de

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Allison, N., M. J. O'Donnell, M. E. Hoey, and C. A. Fewson. "Membrane-bound lactate dehydrogenases and mandelate dehydrogenases of Acinetobacter calcoaceticus. Location and regulation of expression." Biochemical Journal 227, no. 3 (1985): 753–57. http://dx.doi.org/10.1042/bj2270753.

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Acinetobacter calcoaceticus possesses an L(+)-lactate dehydrogenase and a D(-)-lactate dehydrogenase. Results of experiments in which enzyme activities were measured after growth of bacteria in different media indicated that the two enzymes were co-ordinately induced by either enantiomer of lactate but not by pyruvate, and repressed by succinate or L-glutamate. The two lactate dehydrogenases have very similar properties to L(+)-mandelate dehydrogenase and D(-)-mandelate dehydrogenase. All four enzymes are NAD(P)-independent and were found to be integral components of the cytoplasmic membrane.

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Basavaraju, Sreedevi, Chandrasekhar Kathera, and Pramoda Kumari Jasti. "Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant." Journal of Microbiology and Biotechnology Research 7, no. 4 (2017): 1. http://dx.doi.org/10.24896/jmbr.2017741.

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The alkaline protease produced by Bacillus cereus UV-15 mutant was purified by precipitation with ammonium sulphate and gel filtration through sephadex G-100. The enzyme has shown to have a molecular weight of 29kDa by SDS polyacrylamide gel electrophoresis. The extracted protease enzyme was purified by 16.64 fold through ammonium sulphate precipitation and chromatography separation in Sephadex G-100. The purified protease had a specific activity of 2915 (U/mg). The zymogram also revealed a clear hydrolytic zone due to proteolytic activity, which coincided with the band obtained with SDS–PAGE.

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Otzen, Daniel E. "Protein Unfolding in Detergents: Effect of Micelle Structure, Ionic Strength, pH, and Temperature." Biophysical Journal 83, no. 4 (2002): 2219–30. http://dx.doi.org/10.1016/s0006-3495(02)73982-9.

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Hakami, Abdulrahim R., Jonathan K. Ball, and Alexander W. Tarr. "Non-ionic detergents facilitate non-specific binding of M13 bacteriophage to polystyrene surfaces." Journal of Virological Methods 221 (September 2015): 1–8. http://dx.doi.org/10.1016/j.jviromet.2015.04.023.

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Antonets, K. S., K. V. Volkov, A. L. Maltseva, L. M. Arshakian, A. P. Galkin, and A. A. Nizhnikov. "Proteomic analysis of Escherichia coli protein fractions resistant to solubilization by ionic detergents." Biochemistry (Moscow) 81, no. 1 (2016): 34–46. http://dx.doi.org/10.1134/s0006297916010041.

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INDIG, Fred E., Federico DIAZ-GONZALEZ та Mark H. GINSBERG. "Analysis of the tetraspanin CD9–integrin αIIbβ3 (GPIIb-IIIa) complex in platelet membranes and transfected cells". Biochemical Journal 327, № 1 (1997): 291–98. http://dx.doi.org/10.1042/bj3270291.

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The platelet integrin, αIIbβ3 (GPIIb-IIIa), and the tetraspanin, CD9, are integral membrane proteins that are abundant in platelet membranes. We have identified several proteins, including CD9, which were co-precipitated by anti-αIIbβ3 antibody from untreated, resting platelets that were solubilized with the poly(oxyethylene) non-ionic detergent, Brij-35. Immunoblot and quantitative immunoprecipitation showed that the association of αIIbβ3 with CD9 is specific and stoichiometric. The interaction between CD9 and αIIbβ3 is probably hydrophobic, as Triton X-100 and hydrophobic detergents of the B

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Ramzan, Rabia, Jörg Napiwotzki, Petra Weber, Bernhard Kadenbach, and Sebastian Vogt. "Cholate Disrupts Regulatory Functions of Cytochrome c Oxidase." Cells 10, no. 7 (2021): 1579. http://dx.doi.org/10.3390/cells10071579.

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Cytochrome c oxidase (CytOx), the oxygen-accepting and rate-limiting enzyme of mitochondrial respiration, binds with 10 molecules of ADP, 7 of which are exchanged by ATP at high ATP/ADP-ratios. These bound ATP and ADP can be exchanged by cholate, which is generally used for the purification of CytOx. Many crystal structures of isolated CytOx were performed with the enzyme isolated from mitochondria using sodium cholate as a detergent. Cholate, however, dimerizes the enzyme isolated in non-ionic detergents and induces a structural change as evident from a spectral change. Consequently, it turns

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Hare, J. F., and A. Holocher. "Solubility in non-ionic detergents distinguishes between slowly and rapidly degraded plasma membrane proteins." Journal of Biological Chemistry 269, no. 8 (1994): 5981–88. http://dx.doi.org/10.1016/s0021-9258(17)37559-2.

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Nyberg, Harri. "The influence of ionic detergents on the phospholipid fatty acid compositions of Porphyridium purpureum." Phytochemistry 24, no. 3 (1985): 435–40. http://dx.doi.org/10.1016/s0031-9422(00)80742-6.

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Doñate, Fernando, Antonio Artigues, Ana Iriarte, and Marino Martinez-Carrion. "Opposite behavior of two isozymes when refolding in the presence of non-ionic detergents." Protein Science 7, no. 8 (1998): 1811–20. http://dx.doi.org/10.1002/pro.5560070817.

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Wosiewitz, U., and U. Leuschner. "Solubilization of unconjugated bilirubin and its calcium salts by ionic, amphoteric, and nonionic detergents." Naturwissenschaften 77, no. 5 (1990): 232–34. http://dx.doi.org/10.1007/bf01138490.

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Bertolotti, S. G., O. E. Zimerman, J. J. Cosa, and C. M. Previtali. "Excimer emission of pyrene derivatives induced by ionic detergents below the critical micelle concentration." Journal of Luminescence 55, no. 2 (1993): 105–13. http://dx.doi.org/10.1016/0022-2313(93)90015-f.

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Kriechbaum, Manfred, Otto S. Wolfbeis, and Ernst Koller. "Evaluation of critical micelle concentrations of non-ionic detergents using new superpolar lipid probes." Chemistry and Physics of Lipids 44, no. 1 (1987): 19–29. http://dx.doi.org/10.1016/0009-3084(87)90003-x.

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Cohen, A. "Effects of Some Industrial Chemicals on Anaerobic Activity Measured by Sequential Automated Methanometry (SAM)." Water Science and Technology 25, no. 7 (1992): 11–20. http://dx.doi.org/10.2166/wst.1992.0134.

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An experimental study was conducted into the effects of exposure of anaerobic bacteria to some commercial industrial chemicals. Anaerobic activity was tested using Sequential Automated Methanometry (SAM). SAM measures small pressure increases caused by gas production in vials containing anaerobic bacteria. Tested were bleaching agents including hydrogen peroxide and sodium metabisulphite, mothproofing and insect repelling agents containing synthetic pyrethroids, a bacteriostatic agent and non-ionic detergents commonly used in the wool scouring industry. Actively digesting bacterial material wa

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PRINCE, Lawrence S., and Michael J. WELSH. "Cell surface expression and biosynthesis of epithelial Na+ channels." Biochemical Journal 336, no. 3 (1998): 705–10. http://dx.doi.org/10.1042/bj3360705.

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The epithelial Na+ channel (ENaC) complex is composed of three homologous subunits: α, β and γ. Mutations in ENaC subunits can increase the number of channels on the cell surface, causing a hereditary form of hypertension called Liddle's syndrome, or can decrease channel activity, causing pseudohypoaldosteronism type I, a salt-wasting disease of infancy. To investigate surface expression, we studied ENaC subunits expressed in COS-7 and HEK293 cells. Using surface biotinylation and protease sensitivity, we found that when individual ENaC subunits are expressed alone, they traffic to the cell su

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Dorosz, Urszula, Natalia Barteczko, Piotr Latos, Karol Erfurt, Ewa Pankalla, and Anna Chrobok. "Highly Efficient Biphasic System for the Synthesis of Alkyl Lactates in the Presence of Acidic Ionic Liquids." Catalysts 10, no. 1 (2019): 37. http://dx.doi.org/10.3390/catal10010037.

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Alkyl lactates are produced from lactic acid via esterification, and are used in the production of plastics, paints, solvents and detergents. In the pursuit of an inexpensive, industry-suitable catalyst for this reaction, the application of protic ionic liquids based on nitrogen base and sulphuric acid is proposed. The ionic liquid was synthesised via a simple reaction of triethylamine and a threefold molar excess of sulphuric acid. Water was added to remove the heat of the reaction. Next, the reaction conditions for the model esterification of 2-ethylhexanol with lactic acid without additiona

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Zaki, Doreya, Mostafa Abdel-Aziz, Mohamed Naguib, and Ahmed Shalabi. "Effect of Non-ionic Detergents and Vitamins on the Amino Acid Synthesis by Brevibacterium ammoniagenes." Zentralblatt für Mikrobiologie 142, no. 4 (1987): 333–39. http://dx.doi.org/10.1016/s0232-4393(87)80056-2.

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Okubo, Tsuneo, Yasushi Maeda, and Hiromi Kitano. "Inclusion process of ionic detergents with cyclodextrins as studied by the conductance stopped-flow method." Journal of Physical Chemistry 93, no. 9 (1989): 3721–23. http://dx.doi.org/10.1021/j100346a069.

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