Academic literature on the topic 'IRFP720'

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Journal articles on the topic "IRFP720"

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Mezzanotte, Laura, Juvita Delancy Iljas, Ivo Que, et al. "Optimized Longitudinal Monitoring of Stem Cell Grafts in Mouse Brain Using a Novel Bioluminescent/Near Infrared Fluorescent Fusion Reporter." Cell Transplantation 26, no. 12 (2017): 1878–89. http://dx.doi.org/10.1177/0963689717739718.

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Biodistribution and fate of transplanted stem cells via longitudinal monitoring has been successfully achieved in the last decade using optical imaging. However, sensitive longitudinal imaging of transplanted stem cells in deep tissue like the brain remains challenging not only due to low light penetration but because of other factors such as low or inferior expression levels of optical reporters in stem cells and stem cell death after transplantation. Here we describe an optimized imaging protocol for sensitive long-term monitoring of bone marrow-derived human mesenchymal stem cells (hMSCs) e
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Leben, Ruth, Randall L. Lindquist, Anja E. Hauser, Raluca Niesner, and Asylkhan Rakhymzhan. "Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range." International Journal of Molecular Sciences 23, no. 21 (2022): 13407. http://dx.doi.org/10.3390/ijms232113407.

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Two-photon excitation fluorescence laser-scanning microscopy is the preferred method for studying dynamic processes in living organ models or even in living organisms. Thanks to near-infrared and infrared excitation, it is possible to penetrate deep into the tissue, reaching areas of interest relevant to life sciences and biomedicine. In those imaging experiments, two-photon excitation spectra are needed to select the optimal laser wavelength to excite as many fluorophores as possible simultaneously in the sample under consideration. The more fluorophores that can be excited, and the more cell
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Wilson, Amy, Kirsty Wilson, Maree Bilandzic, et al. "Non-Invasive Fluorescent Monitoring of Ovarian Cancer in an Immunocompetent Mouse Model." Cancers 11, no. 1 (2018): 32. http://dx.doi.org/10.3390/cancers11010032.

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Ovarian cancers (OCs) are the most lethal gynaecological malignancy, with high levels of relapse and acquired chemo-resistance. Whilst the tumour–immune nexus controls both cancer progression and regression, the lack of an appropriate system to accurately model tumour stage and immune status has hampered the validation of clinically relevant immunotherapies and therapeutic vaccines to date. To address this need, we stably integrated the near-infrared phytochrome iRFP720 at the ROSA26 genomic locus of ID8 mouse OC cells. Intrabursal ovarian implantation into C57BL/6 mice, followed by regular, n
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Ogawa, Katsuhiro, Kentaro Yamada, Tsuyoshi Etoh, et al. "Development of an oncolytic mammalian orthoreovirus expressing the near-infrared fluorescent protein iRFP720." Journal of Virological Methods 308 (October 2022): 114574. http://dx.doi.org/10.1016/j.jviromet.2022.114574.

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Isomura, Minori, Kentaro Yamada, Kazuko Noguchi, and Akira Nishizono. "Near-infrared fluorescent protein iRFP720 is optimal for in vivo fluorescence imaging of rabies virus infection." Journal of General Virology 98, no. 11 (2017): 2689–98. http://dx.doi.org/10.1099/jgv.0.000950.

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Fukuda, Aya, Shiho Honda, Norie Fujioka, et al. "Non-invasive in vivo imaging of UCP1 expression in live mice via near-infrared fluorescent protein iRFP720." PLOS ONE 14, no. 11 (2019): e0225213. http://dx.doi.org/10.1371/journal.pone.0225213.

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Hu, Han, Ziyi Zhang, Runyang Wang, et al. "BGC823 Cell Line with the Stable Expression of iRFP720 Retains Its Primary Properties with Promising Fluorescence Imaging Ability." DNA and Cell Biology 39, no. 5 (2020): 900–908. http://dx.doi.org/10.1089/dna.2019.5057.

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Chan, Xin Hui Derryn, Ghayathri Balasundaram, Amalina Binte Ebrahim Attia, et al. "Multimodal imaging approach to monitor browning of adipose tissue in vivo." Journal of Lipid Research 59, no. 6 (2018): 1071–78. http://dx.doi.org/10.1194/jlr.d083410.

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The discovery that white adipocytes can undergo a browning process to become metabolically active beige cells has attracted significant interest in the fight against obesity. However, the study of adipose browning has been impeded by a lack of imaging tools that allow longitudinal and noninvasive monitoring of this process in vivo. Here, we report a preclinical imaging approach to detect development of beige adipocytes during adrenergic stimulation. In this approach, we expressed near-infrared fluorescent protein, iRFP720, driven under an uncoupling protein-1 (Ucp1) promoter in mice by viral t
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Stepanenko, Olesya V., Olga V. Stepanenko, Olesya G. Shpironok, Alexander V. Fonin, Irina M. Kuznetsova, and Konstantin K. Turoverov. "Near-Infrared Markers based on Bacterial Phytochromes with Phycocyanobilin as a Chromophore." International Journal of Molecular Sciences 20, no. 23 (2019): 6067. http://dx.doi.org/10.3390/ijms20236067.

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Biomarkers engineered on the basis of bacterial phytochromes with biliverdin IXα (BV) cofactor as a chromophore are increasingly used in cell biology and biomedicine, since their absorption and fluorescence spectra lie within the so-called optical “transparency window” of biological tissues. However, the quantum yield of BV fluorescence in these biomarkers does not exceed 0.145. The task of generating biomarkers with a higher fluorescence quantum yield remains relevant. To address the problem, we proposed the use of phycocyanobilin (PCB) as a chromophore of biomarkers derived from bacterial ph
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Степаненко, Ольга В., та Олеся В. Степаненко. "АГРЕГАЦИЯ БЛИЖНЕ-ИНФРАКРАСНОГО ФЛУОРЕСЦЕНТНОГО БЕЛКА iRFP713 ПРИ РАЗВОРАЧИВАНИИ, "Цитология"". Tsitologiya, № 10 (2018): 842–46. http://dx.doi.org/10.7868/s004137711810017x.

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В настоящей работе мы исследовали процессы разворачивания-сворачивания белка iRFP713, принадлежащего к классу ближне-инфракрасных флуоресцентных маркеров (NIR FPs), широко используемых для исследования молекулярных процессов отдельной клетки и визуализации тканей и органов целого организма в реальном масштабе времени. Разворачивание iRFP713 в апоформе (в отсутствие хромоформа) и в холоформе (в комплексе с биливердином) под действием сильного химического денатуранта гуанидинтиоцианата (GTC) начинается с диссоциации димера белка на мономеры. Агрегация iRFP713 при использовании GTC обусловлена со
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Dissertations / Theses on the topic "IRFP720"

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Tong, Xinlin. "Mechanisms of action of Dipeptidyl Peptidase 9 in liver cancer." Thesis, The University of Sydney, 2020. https://hdl.handle.net/2123/24732.

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Dipeptidyl peptidase 9 (DPP9) is an emerging cancer associated protease, which has unclear roles in hepatocellular carcinoma (HCC). DPP9 has important roles in DNA Double-Strand Break Repair (DSBR), inflammasome and immunoregulation, metabolism, and disease pathogenesis. Here we used hepatocyte-specific gene knock-out and enzymatic deficiency strategies to characterise the roles of DPP9 in the context of DNA Double-Strand Breaks (DSBs) and HCC. DPP9 gki (DPP9 enzyme-negative gene-knock-in; DPP9S729A/S729A) Mouse Embryonic Fibroblasts (MEFs) had been derived from DPP9S729A/S729A mice. Compar
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Wang, Hui [Verfasser], Martin [Akademischer Betreuer] Klingenspor, Thomas [Gutachter] Skurk, and Martin [Gutachter] Klingenspor. "Phenotypic characterization of a novel Ucp1-LUC-iRFP713 reporter mouse model for visualization and quantification of brown and beige fat recruitment / Hui Wang ; Gutachter: Thomas Skurk, Martin Klingenspor ; Betreuer: Martin Klingenspor." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1211725154/34.

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Conference papers on the topic "IRFP720"

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Lashuk, Kanstantsin, Jacqueline Bersano, Dorothee Lenhard, Eva Oswald, Kerstin Klingner, and Julia Schüler. "Abstract 2806: Development of an iRFP713-based orthotopic patient derived brain cancer model to study tumor development in vivo." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-2806.

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