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Journal articles on the topic 'IS1081'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Firoz, Shehraz, and Sonia Bhatt. "Diagnostic value of polymerase chain reaction targeting insertion sequence IS1081 for the diagnosis of pediatric tuberculosis." International Journal of Contemporary Pediatrics 7, no. 4 (2020): 905. http://dx.doi.org/10.18203/2349-3291.ijcp20201152.

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Background: Aim of this study was to evaluate the efficacy of PCR targeting IS1081in diagnosis of pediatric tuberculosis and compare the results with MGIT culture.Methods: This prospective study was conducted in the department of pediatrics, S.N. medical college, Agra. 100 subjects (28 pulmonary 72 extra pulmonary) were registered in study. The specimens obtained from these cases were subjected to Ziehl–Neelsen staining (ZN), MGIT 960 TB culture and PCR targeting insertion sequence IS1081. Sensitivity, specificity, PPV and NPV of PCR were calculated in pulmonary and extra pulmonary specimens.

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2

Sota, Masahiro, Masahiro Endo, Keiji Nitta, Haruhiko Kawasaki, and Masataka Tsuda. "Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1." Applied and Environmental Microbiology 68, no. 5 (2002): 2307–15. http://dx.doi.org/10.1128/aem.68.5.2307-2315.2002.

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ABSTRACT The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (TnHad1) that was flanked by two directly repeated copies of IS1071, IS1071L and IS1071R. The dehH1 gene was also flanked by IS1071L and a truncated version of IS1071 (IS1071N). TnHad1, dehH1, and IS1071N were located on a 15.6-kb class II transposon (TnHad2) whose terminal inverted repeats and res site showed high homology with those of the Tn21-relat

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3

Collins, Desmond M., and Diana M. Stephens. "Identification of an insertion sequence, IS1081, inMycobacterium bovis." FEMS Microbiology Letters 83, no. 1 (1991): 11–16. http://dx.doi.org/10.1111/j.1574-6968.1991.tb04380.x.

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4

Selim, A., M. El-Haig, and W. Gaede. "Duplex real-time PCR assay targeting insertion elements IS1081 and IS6110 for detection of Mycobacterium bovis in lymph nodes of cattle." Biotehnologija u stocarstvu 30, no. 1 (2014): 45–59. http://dx.doi.org/10.2298/bah1401045s.

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The development of a reliable and rapid screening test for detection of Mycobacterium bovis (M. bovis) helps in control of bovine tuberculosis. The aim of this study was evaluate a sensitive and specific assay for detecting M. bovis DNA in lymph nodes with lesions suggestive to tuberculosis taken from slaughtered cattle. A duplex real-time PCR assay was developed for the identification of M. bovis targeting insertion elements (IS) IS1081 and IS6110 in one internally controlled reaction. M. bovis DNA extraction protocols from tissue samples were evaluated. The specificity and sensitivity of the

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5

Abdelfattah, Selim, El haig Mahmoud, and Youssef Ahmad. "A Sensitive and Specific Duplex Real-Time PCR Assay Targeting Insertion Elements IS1081 and IS6110 for Detection of Mycobacterium bovis in Lymph Nodes of Cattle." Global Animal Science Journal 1, no. 1 (2013): 1100–1114. https://doi.org/10.5281/zenodo.19332.

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The development of a reliable and rapid screening test for detection of <em>Mycobacterium bovis </em>(<em>M. bovis</em>) helps to control of bovine tuberculosis and preventing zoonotic infections. This study aimed to evaluate a sensitive and specific assay for detecting <em>M. bovis</em> DNA in lymph nodes with lesions suggestive to tuberculosis taken from slaughtered cattle. A duplex real-time PCR assay was developed for the identification of <em>M. bovis</em> targeting insertion elements (IS) IS1081 and IS 6110 in one internally controlled reaction. <em>M. bovis</em> DNA extraction protocols

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Abdelfattah, Selim, Elhaig Mahmoud, and Youssef Ahmad. "A Sensitive and Specific Duplex Real-Time PCR Assay Targeting Insertion Elements IS1081 and IS6110 for Detection of Mycobacterium bovis in Lymph Nodes of Cattle." Global Animal Science Journal 1, no. 1 (2013): 1–10. https://doi.org/10.5281/zenodo.23779.

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The development of a reliable and rapid screening test for detection of <em>Mycobacterium bovis </em>(<em>M. bovis</em>) helps to control of bovine tuberculosis and preventing zoonotic infections. This study aimed to evaluate a sensitive and specific assay for detecting <em>M. bovis</em> DNA in lymph nodes with lesions suggestive to tuberculosis taken from slaughtered cattle. A duplex real-time PCR assay was developed for the identification of <em>M. bovis</em> targeting insertion elements (IS) IS1081 and IS 6110 in one internally controlled reaction. <em>M. bovis</em> DNA extraction protocols

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7

Ahmed, Niyaz, Ashok Kumar Mohanty, Utpal Mukhopadhyay, Virender Kumar Batish, and Sunita Grover. "PCR-Based Rapid Detection of Mycobacterium tuberculosis in Blood from Immunocompetent Patients with Pulmonary Tuberculosis." Journal of Clinical Microbiology 36, no. 10 (1998): 3094–95. http://dx.doi.org/10.1128/jcm.36.10.3094-3095.1998.

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A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for the circulating DNA copies of M. tuberculosis complex.

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8

Podglajen, I., J. Breuil, A. Rohaut, C. Monsempes, and E. Collatz. "Multiple Mobile Promoter Regions for the Rare Carbapenem Resistance Gene of Bacteroides fragilis." Journal of Bacteriology 183, no. 11 (2001): 3531–35. http://dx.doi.org/10.1128/jb.183.11.3531-3535.2001.

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ABSTRACT Two novel insertion sequences (IS), IS1187 and IS1188, are described upstream from the carbapenem resistance gene cfiA in strains of Bacteroides fragilis. Mapping, with the RACE procedure, of transcription start sites of cfiA in these and two other previously reported IS showed that transcription of this rarely encountered gene is initiated close to a variety of B. fragilis consensus promoter sequences, as recently defined (D. P. Bayley, E. R. Rocha, and C. J. Smith, FEMS Microbiol. Lett. 193:149–154, 2000). In the cases of IS1186 and IS1188, these sequences overlap with putative Eς70

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9

Zeineldin, Mohamed M., Kimberly Lehman, Patrick Camp, David Farrell, and Tyler C. Thacker. "Diagnostic Evaluation of the IS1081-Targeted Real-Time PCR for Detection of Mycobacterium bovis DNA in Bovine Milk Samples." Pathogens 12, no. 8 (2023): 972. http://dx.doi.org/10.3390/pathogens12080972.

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The ability of Mycobacterium bovis (M. bovis) to survive in bovine milk has emerged as a serious public health concern. The first objective of this study was to evaluate the diagnostic utility of IS1081-targeted real-time PCR for the detection of M. bovis DNA in different fractions of bovine milk. In a model study, bovine milk samples were spiked with serially diluted M. bovis BCG to investigate the detection limit of M. bovis DNA in whole milk and milk fractions (cream, pellet, and pellet + cream combined) using IS1081 real-time PCR. The assay was then used to detect M. bovis DNA in whole mil

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10

Huh, Y. J., D. I. Ahn, and S. J. Kim. "Limited variation of DNA fingerprints (IS6110 and IS1081) in Korean strains of Mycobacterium tuberculosis." Tubercle and Lung Disease 76, no. 4 (1995): 324–29. http://dx.doi.org/10.1016/s0962-8479(05)80031-0.

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11

Serraino, Andrea, Giulia Marchetti, Valeria Sanguinetti, et al. "Monitoring of Transmission of Tuberculosis between Wild Boars and Cattle: Genotypical Analysis of Strains by Molecular Epidemiology Techniques." Journal of Clinical Microbiology 37, no. 9 (1999): 2766–71. http://dx.doi.org/10.1128/jcm.37.9.2766-2771.1999.

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An epidemiological survey for the monitoring of bovine tuberculosis transmission was carried out in western Liguria, a region in northern Italy. Fifteen Mycobacterium bovis strains were isolated from 63 wild boar samples (62 from mandibular lymph nodes and 1 from a liver specimen). Sixteen mediastinal lymph nodes of 16 head of cattle were collected, and 15 Mycobacterium bovis strains were subsequently cultured. All M. bovis strains isolated from cattle and wild boars were genotyped by spoligotyping and by restriction fragment length polymorphism (RFLP) analysis with the IS6110 and IS1081 probe

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12

Traversa, María Julia, Ignacio Etchechoury, María Cristina Jorge, et al. "Isolamento de micobactérias em Felis concolor em cativeiro." Brazilian Journal of Veterinary Research and Animal Science 46, no. 1 (2009): 25. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2009.26746.

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Este trabalho foi realizado em uma reserva natural da Argentina com antecedentes de tuberculose em uma suçuarana adulta. O objetivo foi identificar por meio de técnicas bacteriológicas e de biologia molecular as espécies isoladas da orofaringe de cinco suçuaranas que apresentavam sinais clínicos inespecíficos. As amostras foram colhidas das suçuaranas após sedação. Posteriormente foram processadas para obtenção do isolamento e identificação por meio de provas bioquímicas do gênero Mycobacterium pela técnica de PCR. Investigou-se a presença das seqüências de inserção IS6110 e IS1081 e hsp65. Ob

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Collins, D. M., S. K. Erasmuson, D. M. Stephens, G. F. Yates, and G. W. De Lisle. "DNA fingerprinting of Mycobacterium bovis strains by restriction fragment analysis and hybridization with insertion elements IS1081 and IS6110." Journal of Clinical Microbiology 31, no. 5 (1993): 1143–47. http://dx.doi.org/10.1128/jcm.31.5.1143-1147.1993.

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14

Di Gioia, Diana, Michelle Peel, Fabio Fava, and R. Campbell Wyndham. "Structures of Homologous Composite Transposons Carrying cbaABC Genes from Europe and North America." Applied and Environmental Microbiology 64, no. 5 (1998): 1940–46. http://dx.doi.org/10.1128/aem.64.5.1940-1946.1998.

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ABSTRACT IS1071 is a class II transposable element carrying atnpA gene related to the transposase genes of the Tn3 family. Copies of IS1071 that are conserved with more than 99% nucleotide sequence identity have been found as direct repeats flanking a remarkable variety of catabolic gene sequences worldwide. The sequences of chlorobenzoate catabolic transposons found on pBRC60 (Tn5271) in Niagara Falls, N.Y., and on pCPE3 in Bologna, Italy, show that these transposons were formed from highly homologous IS1071 and cbaABCcomponents (levels of identity, >99.5 and >99.3%, respectively). Neve

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15

van Soolingen, D., P. W. Hermans, P. E. de Haas, and J. D. van Embden. "Insertion element IS1081-associated restriction fragment length polymorphisms in Mycobacterium tuberculosis complex species: a reliable tool for recognizing Mycobacterium bovis BCG." Journal of Clinical Microbiology 30, no. 7 (1992): 1772–77. http://dx.doi.org/10.1128/jcm.30.7.1772-1777.1992.

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16

RĂȚOI, Ioana Alexandra, Luanda Elena OȘLOBANU, Dragoș Constantin ANIȚĂ, et al. "IDENTIFICATION OF MYCOBACTERIUM CAPRAE FROM MINKS." Scientific Papers Journal VETERINARY SERIES 67, no. 4 (2024): 58–61. https://doi.org/10.61900/spjvs.2024.04.10.

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Mycobacterium caprae (M. caprae) is a member of Mycobacterium tuberculosis Complex (MTC) and one of the causative agents of bovine tuberculosis within animal populations. The species was linked to outbreaks of tuberculosis in central and western European countries, mainly in cattle and goat, but also in wildlife and human. In our country, in recent years, M. caprae was the predominant identified species in infected cattle herds. Genotyping of mycobacteria strains can facilitate the study of the dynamics of bovine tuberculosis in Romania, allowing the traceability of the outbreaks. The aim of t

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17

Nyaruaba, Raphael, Jin Xiong, Caroline Mwaliko, et al. "Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Mycobacterium tuberculosis." Microorganisms 8, no. 5 (2020): 701. http://dx.doi.org/10.3390/microorganisms8050701.

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Droplet digital PCR (ddPCR) is a third generation of PCR that was recently developed to overcome the challenges of real-time fluorescence-based quantitative PCR (qPCR) in absolute quantification of pathogens. Few studies have been done on tuberculosis (TB) detection and quantification using ddPCR despite its many advantages over qPCR. From the few studies, none explores a single dye duplex assay for the detection and quantification of TB. In this study, steps toward developing and evaluating a duplex single dye (FAM) assay for detecting two targets (IS6110 and IS1081) are clearly described usi

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18

Nghiem, Minh Ngoc, Bac Van Nguyen, Son Thai Nguyen, Thuy Thi Bich Vo, and Hai Van Nong. "A Simple, Single Triplex PCR of IS6110, IS1081, and 23S Ribosomal DNA Targets, Developed for Rapid Detection and Discrimination of Mycobacterium from Clinical Samples." Journal of Microbiology and Biotechnology 25, no. 5 (2015): 745–52. http://dx.doi.org/10.4014/jmb.1409.09089.

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19

Sola, Christophe, Ingrid Filliol, Eric Legrand, Igor Mokrousov, and Nalin Rastogi. "Mycobacterium tuberculosis Phylogeny Reconstruction Based on Combined Numerical Analysis with IS1081, IS6110, VNTR, and DR-Based Spoligotyping Suggests the Existence of Two New Phylogeographical Clades." Journal of Molecular Evolution 53, no. 6 (2001): 680–89. http://dx.doi.org/10.1007/s002390010255.

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20

Derbise, A., S. Aubert, and N. El Solh. "Mapping the regions carrying the three contiguous antibiotic resistance genes aadE, sat4, and aphA-3 in the genomes of staphylococci." Antimicrobial Agents and Chemotherapy 41, no. 5 (1997): 1024–32. http://dx.doi.org/10.1128/aac.41.5.1024.

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Tn5405 (12 kb) is a staphylococcal composite transposon delimited by two inverted copies of IS1182, one of which contains IS1181. The internal part of this transposon carries three antibiotic resistance genes, aphA-3, aadE, and sat4, and three open reading frames (ORFs), orfx, orfy, and orfz, of unknown function. The dispersion of Tn5405 and the genes and ORFs included in this transposon were investigated in 50 epidemiologically unrelated staphylococci carrying aphA-3. Twenty-three maps, distinguishable by the presence or absence of the investigated genes or ORFs and/or by the sizes of the res

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21

Hoegh, Silje V., Charlotte N. Agergaard, Marianne N. Skov, and Michael Kemp. "False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients." Open Microbiology Journal 13, no. 1 (2019): 51–54. http://dx.doi.org/10.2174/1874285801913010051.

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Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion seq

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Sharma, Anchal, Kusum Sharma, Manish Modi, and Aman Sharma. "1633. Improving the diagnosis of extra-pulmonary tuberculosis (EPTB): experience from north India." Open Forum Infectious Diseases 7, Supplement_1 (2020): S808. http://dx.doi.org/10.1093/ofid/ofaa439.1813.

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Abstract Background Rapid and accurate diagnosis of extra-pulmonary tuberculosis (EPTB) is imperative for early treatment and better patient outcome. Loop-mediated Isothermal Amplification (LAMP) is a promising nucleic-acid amplification assay. LAMP assay could be carried out in simple water bath under isothermal conditions in 60 minutes, and can be performed in any laboratory even in rural setting in resource poor endemic countries. We evaluated LAMP assay using two different target regions LAMP primers specific for Mycobacterium tuberculosis complex for the diagnosis of EPTB. Methods LAMP as

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Fang, Z., N. Morrison, B. Watt, C. Doig, and K. J. Forbes. "IS6110 Transposition and Evolutionary Scenario of the Direct Repeat Locus in a Group of Closely Related Mycobacterium tuberculosis Strains." Journal of Bacteriology 180, no. 8 (1998): 2102–9. http://dx.doi.org/10.1128/jb.180.8.2102-2109.1998.

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ABSTRACT In recent years, various polymorphic loci and multicopy insertion elements have been discovered in the Mycobacterium tuberculosis genome, such as the direct repeat (DR) locus, the major polymorphic tandem repeats, the polymorphic GC-rich repetitive sequence, IS6110, and IS1081. These, especially IS6110 and the DR locus, have been widely used as genetic markers to differentiate M. tuberculosis isolates and will continue to be so used, due to the conserved nature of the genome ofM. tuberculosis. However, little is known about the processes involved in generating these or of their relati

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JAVED, S., F. SAID, S. A. M. A. S. EQANI, and H. BOKHARI. "Bordetella parapertussis outbreak in Bisham, Pakistan in 2009–2010: fallout of the 9/11 syndrome." Epidemiology and Infection 143, no. 12 (2015): 2619–23. http://dx.doi.org/10.1017/s0950268814003732.

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SUMMARYPertussis or whooping cough is a highly contagious community disease mainly caused by Bordetella pertussis and B. parapertussis. We report a minor outbreak of whooping cough (2009–2010) in symptomatic subjects from Bisham, near Swat, Khyber Pukhtoonkhawa province, Pakistan. Interestingly, our results show that all the culture-positive isolates (n = 21) collected from children (average age 3·46 years), were identified as B. parapertussis after routine identification tests and PCR IS481, IS1001 and IS1002. Furthermore, in the affected patients, none had received immunization with diphther

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Cornejo-Granados, Fernanda, Gamaliel López-Leal, Dulce A. Mata-Espinosa, et al. "Targeted RNA-Seq Reveals the M. tuberculosis Transcriptome from an In Vivo Infection Model." Biology 10, no. 9 (2021): 848. http://dx.doi.org/10.3390/biology10090848.

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The study of host-pathogen interactions using in vivo models with intracellular pathogens like Mycobacterium tuberculosis (Mtb) entails technical limitations, such as: (i) Selecting an efficient differential lysis system to enrich the pathogen cells; (ii) obtaining sufficient high-quality RNA; and (iii) achieving an efficient rRNA depletion. Thus, some authors had used flow cytometers to separate infected cells or significantly increase the sequencing depth of host–pathogen RNA libraries to observe the pathogens’ gene expression. However, these options carry additional expenses in specialized

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26

Siriwut, Warut, Gregory D. Edgecombe, Chirasak Sutcharit, Piyoros Tongkerd, and Somsak Panha. "Systematic revision and phylogenetic reassessment of the centipede genera Rhysida Wood, 1862 and Alluropus Silvestri, 1912 (Chilopoda: Scolopendromorpha) in Southeast Asia, with further discussion of the subfamily Otostigminae." Invertebrate Systematics 32, no. 5 (2018): 1005. http://dx.doi.org/10.1071/is17081.

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Phylogenetic relationships of two morphologically similar scolopendrid genera, Rhysida Wood, 1862, and Alluropus Silvestri, 1912, were investigated based on broad-scale taxonomic sampling from SE Asia, India and Australia. Morphological revision and molecular phylogenetics using three loci validate seven Rhysida species in SE Asia and Australia: R. lithobioides (Newport, 1845), R. longipes (Newport, 1845), R. immarginata (Porat, 1876), R. nuda (Newport, 1845), R. carinulata (Haase, 1887), R. singaporiensis Verhoeff, 1937 and R. polyacantha Koch, 1985. The nominal SE Asian species R. leviventer

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Sota, Masahiro, Hirokazu Yano, Yuji Nagata, et al. "Functional Analysis of Unique Class II Insertion Sequence IS1071." Applied and Environmental Microbiology 72, no. 1 (2006): 291–97. http://dx.doi.org/10.1128/aem.72.1.291-297.2006.

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ABSTRACT Various xenobiotic-degrading genes on many catabolic plasmids are often flanked by two copies of an insertion sequence, IS1071. This 3.2-kb IS element has long (110-bp) terminal inverted repeats (IRs) and a transposase gene that are phylogenetically related to those of the class II transposons. However, the transposition mechanism of IS1071 has remained unclear. Our study revealed that IS1071 was only able to transpose at high frequencies in two environmental β-proteobacterial strains, Comamonas testosteroni and Delftia acidovorans, and not in any of the bacteria examined which belong

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28

Providenti, Miguel A., Rachel E. Shaye, Krista D. Lynes, et al. "The Locus Coding for the 3-Nitrobenzoate Dioxygenase of Comamonas sp. Strain JS46 Is Flanked by IS1071 Elements and Is Subject to Deletion and Inversion Events." Applied and Environmental Microbiology 72, no. 4 (2006): 2651–60. http://dx.doi.org/10.1128/aem.72.4.2651-2660.2006.

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ABSTRACT In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture method, cloned, and sequenced. The 3Nba dioxygenase (MnbA) is a member of the phthalate family of aromatic oxygenases. An open reading frame designated mnbB that codes for an NAD(P)H-dependent class IA aromatic oxidoreductase is downstream of mnbA. MnbB is tentatively ide

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29

Antila, Mia, Qiushui He, Caroline de Jong, et al. "Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis." Journal of Medical Microbiology 55, no. 8 (2006): 1043–51. http://dx.doi.org/10.1099/jmm.0.46331-0.

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Bordetella holmesii is a Gram-negative bacterium first identified in 1995. It can cause pertussis-like symptoms in humans. B. holmesii contains insertion sequences IS481 and IS1001, two frequently used targets in the PCR diagnosis of Bordetella pertussis and Bordetella parapertussis infections. To investigate the prevalence of B. holmesii in Finnish and Dutch patients with pertussis-like symptoms and whether B. holmesii has caused any false-positive results in diagnostic PCRs, B. holmesii-specific real-time PCRs were developed. The Finnish methods were conventional IS481 PCR and B. holmesii-sp

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30

SYMMS, C., B. COOKSON, J. STANLEY, and J. V. HOOKEY. "Analysis of methicillin-resistant Staphylococcus aureus by IS1181 profiling." Epidemiology and Infection 120, no. 3 (1998): 271–79. http://dx.doi.org/10.1017/s0950268898008796.

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Variation in the genomic location and copy number of the insertion element IS1181 in methicillin-resistant Staphylococcus aureus (MRSA) was investigated. Sixty-three isolates representing the Jevons type strain (NCTC 10442), phage-propagating strains, and epidemic strains were examined. A PCR amplicon of the insertion element was used to probe genomic restriction endonuclease digests. HindIII genomic digests gave 25 distinct IS1181 patterns, while EcoRI digests gave 20 patterns. EMRSA-01, -02, -04, -06, -07, -09, -10, -11, -13 and -14 contained the element but could not be subtyped by profilin

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31

Ng, James, and R. Campbell Wyndham. "IS1071-mediated recombinational equilibrium in Alcaligenes sp. BR60 carrying the 3-chlorobenzoate catabolic transposon Tn5271." Canadian Journal of Microbiology 39, no. 1 (1993): 92–100. http://dx.doi.org/10.1139/m93-013.

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In experiments designed to Tn5 mutagenize the indigenous plasmid pBRC60 of Alcaligenes sp. BR60, kanamycin-resistant mutants were isolated that were cured of this plasmid and that exhibited recombination of the plasmid-located chlorobenzoate catabolic transposon Tn5271 into the chromosome. These events were independent of the location of Tn5 insertions into the genome of strain BR60. The chromosomal recombinants carried at least two novel copies of IS1071, the class II insertion sequence flanking Tn5271, compared with the parent strain. Recombination of Tn5271 into the chromosome of Alcaligene

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Satola, Sarah W., Brooke Napier, and Monica M. Farley. "Association of IS1016 with the hia Adhesin Gene and Biotypes V and I in Invasive Nontypeable Haemophilus influenzae." Infection and Immunity 76, no. 11 (2008): 5221–27. http://dx.doi.org/10.1128/iai.00672-08.

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ABSTRACT A subset of invasive nontypeable Haemophilus influenzae (NTHI) strains has evidence of IS1016, an insertion element associated with division I H. influenzae capsule serotypes. We examined IS1016-positive invasive NTHI isolates collected as part of Active Bacterial Core Surveillance within the Georgia Emerging Infections Program for the presence or absence of hmw1 and hmw2 (two related adhesin genes that are common in NTHI but absent in encapsulated H. influenzae) and hia (homologue of hsf, an encapsulated H. influenzae adhesin gene). Isolates were serotyped using slide agglutination,

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Müller, C., U. Lauf, and H. Herrmann. "The inverted repeats of IS1384, a newly described insertion sequence from Pseudomonas putida strain H, represent the specific target for integration of IS1383." Molecular Genetics and Genomics 265, no. 6 (2001): 1004–10. http://dx.doi.org/10.1007/s004380100495.

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Spagna, Joseph C., Sarah C. Crews, and Rosemary G. Gillespie. "Patterns of habitat affinity and Austral/Holarctic parallelism in dictynoid spiders (Araneae:Entelegynae)." Invertebrate Systematics 24, no. 3 (2010): 238. http://dx.doi.org/10.1071/is10001.

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The ability to survive in a terrestrial environment was a major evolutionary hurdle for animals that, once passed, allowed the diversification of most arthropod and vertebrate lineages. Return to a truly aquatic lifestyle has occurred only rarely among terrestrial lineages, and is generally associated with modifications of the respiratory system to conserve oxygen and allow extended periods of apnea. Among chelicerates, in particular spiders, where the circulatory system also serves as a hydrostatic skeleton, very few taxa have exploited aquatic environments, though these environments are abun

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Cook, L. G., R. D. Edwards, M. D. Crisp, and N. B. Hardy. "Need morphology always be required for new species descriptions?" Invertebrate Systematics 24, no. 3 (2010): 322. http://dx.doi.org/10.1071/is10011.

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Despite the widespread and common use of DNA-sequence data to estimate phylogenies, support or contest classifications, and identify species using barcodes, they are not commonly used as the primary or sole source of data for describing species. This is possibly due to actual or perceived pressure from peers to include morphology as the primary source of data for species descriptions. We find no compelling evidence to exclude DNA-only descriptions, or to insist that morphology always be included in a species description. It is not the data type per se that is important, but the science behind

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Bourguignon, Thomas, Rudolf H. Scheffrahn, Jan Křeček, Zoltán T. Nagy, Gontran Sonet, and Yves Roisin. "Towards a revision of the Neotropical soldierless termites (Isoptera:Termitidae): redescription of the genus Anoplotermes and description of Longustitermes, gen. nov." Invertebrate Systematics 24, no. 4 (2010): 357. http://dx.doi.org/10.1071/is10012.

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Soldierless termites are well represented in the Neotropics where they constitute about one-third of the total termite species richness. However, despite their substantial diversity, they have been neglected by most taxonomists because they lack soldiers. Species identification therefore relies upon worker characters, in particular the anatomy of the digestive tract and the enteric valve armature. Here, in order to provide a solid basis for future taxonomic work on this group, we supply detailed descriptions of the type species of the genus Anoplotermes, A. pacificus, and of a few common and w

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Borda, Elizabeth, and Mark E. Siddall. "Insights into the evolutionary history of Indo-Pacific bloodfeeding terrestrial leeches (Hirudinida:Arhynchobdellida:Haemadipisdae)." Invertebrate Systematics 24, no. 5 (2010): 456. http://dx.doi.org/10.1071/is10013.

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Haemadipsidae is a clade of notorious bloodfeeding annelids adapted to tropical and sub-tropical rainforests found throughout the Indo-Pacific. This family traditionally includes duognathous (two-jawed) endemics, each placed in their own genus, from continental and volcanic islands including: Australia, Indonesia, Madagascar, Papua New Guinea, Philippines, Seychelles, and South Pacific islands, while trignathous (three-jawed) Tritetrabdella species and the speciose Haemadipsa are exclusive to the Indian subcontinent ranging into east and south-east Asia. One of the more compelling aspects of h

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Jesse, Ruth, Christoph D. Schubart, and Sebastian Klaus. "Identification of a cryptic lineage within Potamon fluviatile (Herbst) (Crustacea:Brachyura:Potamidae)." Invertebrate Systematics 24, no. 4 (2010): 348. http://dx.doi.org/10.1071/is10014.

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Based on mitochondrial and nuclear DNA-sequence data we identify and describe an evolutionary separate lineage (Potamon pelops, sp. nov.) within the potamid freshwater crab species Potamon fluviatile (Herbst, 1785). So far, Potamon pelops, sp. nov. is only reported from the Peloponnesus Peninsula (Greece), probably allopatric to P. fluviatile. This cryptic lineage is not identical with the infrasubspecies Potamon fluviatile fluviatile natio laconis Pretzmann, 1983, the latter being within the morphological variation of Potamon fluviatile and Potamon pelops. Morphologically, Potamon pelops, sp.

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de Bivort, Benjamin L., and Gonzalo Giribet. "A systematic revision of the South African Pettalidae (Arachnida:Opiliones:Cyphophthalmi) based on a combined analysis of discrete and continuous morphological characters with the description of seven new species." Invertebrate Systematics 24, no. 4 (2010): 371. http://dx.doi.org/10.1071/is10015.

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The cyphophthalmid family Pettalidae in South Africa is revised and seven new species are described from museum material collected between 1939 and 1985. Two of these are placed in the genus Purcellia and five in Parapurcellia, bringing the total number of described South African cyphophthalmids to 15. In addition, Purcellia peregrinator is transferred to the genus Parapurcellia. Phylogenetic analyses of discrete morphological and continuous morphometric characters, both separately and in combination, support the generic assignments and contribute towards a more detailed understanding of the s

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Caron, Edilson, Cibele S. Ribeiro-Costa, and Alfred F. Newton. "Cladistic analysis and revision of Piestus Gravenhorst with remarks on related genera (Coleoptera : Staphylinidae : Piestinae)." Invertebrate Systematics 25, no. 6 (2011): 490. http://dx.doi.org/10.1071/is10016.

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Rove beetles of the genus Piestus Gravenhorst, 1806 are commonly captured under the bark of or inside decaying logs from Neotropical forests. Piestus belongs to the subfamily Piestinae, historically an ill-defined dumping-ground for Staphylinidae defined by plesiomorphic characters, but which has gradually been restricted in concept and currently includes only six additional extant genera worldwide. Piestinae in this restricted sense has been considered a probably monophyletic subfamily, but its status and phylogenetic position, as a possible sister-group of Osoriinae within the recently propo

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Astrin, Jonas J., and Peter E. Stüben. "Molecular phylogeny of Echinodera and Ruteria (Coleoptera:Curculionidae:Cryptorhynchinae) and the parallel speciation of Canary Island weevils along replicate environmental gradients." Invertebrate Systematics 24, no. 5 (2010): 434. http://dx.doi.org/10.1071/is10021.

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A molecular phylogeny for the western Palaearctic weevil genus Echinodera Wollaston, 1863 and the former genus Ruteria Roudier, 1954 is presented, combining two mitochondrial genes (CO1 and 16S) in a Bayesian analysis. Special consideration is given to the species of Echinodera from the Canary Islands. Between islands, these are represented by multiple vicariant species that have undergone parallel speciation along replicate environmental gradients on the respective islands. Based on the phylogenetic tree and further data, a number of taxonomic changes is presented: two new species are describ

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Lattimore, Vanessa L., Cor J. Vink, Adrian M. Paterson, and Robert H. Cruickshank. "Unidirectional introgression within the genus Dolomedes (Araneae:Pisauridae) in southern New Zealand." Invertebrate Systematics 25, no. 1 (2011): 70. http://dx.doi.org/10.1071/is11001.

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We investigated the genetic structure of mitochondrial DNA (COI) and nuclear DNA (actin 5C) for variation within and among populations of two nurseryweb spider species: Dolomedes aquaticus Goyen, 1888 and D. minor Koch, 1876. Specimens were collected from intermediately disturbed braided rivers located in southern South Island, New Zealand. The genetic variation was compared against morphological characteristics to identify traits, both genetically and phenotypically, that indicate past occurrences of introgression. Haplotypes clearly assignable to D. aquaticus were also present in specimens o

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Harvey, Mark S., Michael G. Rix, Volker W. Framenau, et al. "Protecting the innocent: studying short-range endemic taxa enhances conservation outcomes." Invertebrate Systematics 25, no. 1 (2011): 1. http://dx.doi.org/10.1071/is11011.

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A major challenge confronting many contemporary systematists is how to integrate standard taxonomic research with conservation outcomes. With a biodiversity crisis looming and ongoing impediments to taxonomy, how can systematic research continue to document species and infer the ‘Tree of Life’, and still maintain its significance to conservation science and to protecting the very species it strives to understand? Here we advocate a systematic research program dedicated to documenting short-range endemic taxa, which are species with naturally small distributions and, by their very nature, most

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Nielsen, Claus. "How to make a protostome." Invertebrate Systematics 26, no. 1 (2012): 25. http://dx.doi.org/10.1071/is11041.

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The origin and radiation of the major metazoan groups can be elucidated by phylogenomic studies, but morphological evolution must be inferred from embryology and morphology of living organisms. According to the trochaea theory, protostomes are derived from a holoplanktonic gastraea with a circumblastoporal ring of downstream-collecting compound cilia (archaeotroch) and a nervous system comprising an apical ganglion and a circumblastoporal nerve ring. The pelago-benthic life cycle evolved through the addition of a benthic adult stage, with lateral blastopore closure creating a tube-shaped gut.

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Cerretti, Pierfilippo, and Thomas Pape. "Phylogenetics and taxonomy of Ventrops - the largest genus of Afrotropical Rhinophoridae (Diptera)." Invertebrate Systematics 26, no. 3 (2012): 274. http://dx.doi.org/10.1071/is12001.

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Three new species of the Afrotropical genus Ventrops Crosskey, 1977 (V. aethiopicus, sp. nov., V. freidbergi, sp. nov. and V. stuckenbergi, sp. nov.) are described, and all seven known species are included in a morphology-based phylogenetic analysis both to provide a rigorous argumentation for the generic affiliation of the newly described species, and to provide a first explicit hypothesis of phylogenetic relationships between the included species. Also, by including exemplar species from several genera of the Rhinophoridae as outgroups, this analysis is the first explicitly phylogenetic defi

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Glasby, Christopher J., Nu-Wei V. Wei, and Karen S. Gibb. "Cryptic species of Nereididae (Annelida : Polychaeta) on Australian coral reefs." Invertebrate Systematics 27, no. 3 (2013): 245. http://dx.doi.org/10.1071/is12031.

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We investigate the presence of cryptic species among three highly variable nereidid polychaetes commonly found in Australian coral reefs − Nereis denhamensis Augener, 1913, Perinereis suluana (Horst, 1924) and Pseudonereis anomala Gravier, 1901 − based on morphological and molecular data (mitochondrial cytochrome c oxidase subunit I, COI, and nuclear histone H3). DNA extracted and sequenced from 70 specimens from northern Australia and the Philippines indicated the existence of eight species: three matched the types of existing species; four are newly described (Nereis heronensis, sp. nov., N.

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Bernardi, Leopoldo Ferreira de Oliveira, Fernando Augusto Barbosa Silva, Mauricio Sergio Zacarias, Hans Klompen, and Rodrigo Lopes Ferreira. "Phylogenetic and biogeographic analysis of the genus Caribeacarus (Acari : Opilioacarida), with description of a new South American species." Invertebrate Systematics 27, no. 3 (2013): 294. http://dx.doi.org/10.1071/is12041.

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The mite order Opilioacarida is widely distributed in the tropical and sub-tropical zones of the world, where it is rare and poorly known. On the American continent only two genera, 14 species and one subspecies are known. This work aimed to describe a new species of Caribeacarus from the state of Pará, in the eastern part of the Brazilian Amazon. A phylogenetic analysis of the species in this genus is also presented, and interpreted along with the historical biogeography of this genus in Central and South America. A key to the species of Caribeacarus is provided.

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Hernandes, Fabio A., and Reinaldo J. F. Feres. "Phylogeny and taxonomic revision of the spider mite genera Aponychus, Paraponychus and Stylophoronychus using morphology (Acari : Tetranychidae)." Invertebrate Systematics 27, no. 3 (2013): 265. http://dx.doi.org/10.1071/is12071.

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Despite their great importance as pests of cultivated plants worldwide, members of the family Tetranychidae have never been subjected to a thorough cladistic analysis to reveal the history and relationships among related genera. Herein, we provide the first phylogenetic analysis and taxonomic review of species of the genera Aponychus, Paraponychus and Stylophoronychus using morphological characters. The results indicate a monophyletic clade uniting the aforementioned genera, although none of the three genera were recovered as monophyletic. We reinstate the tribe Aponychini as the taxon contain

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Karanovic, Tomislav, Stefan M. Eberhard, Giulia Perina, and Shae Callan. "Two new subterranean ameirids (Crustacea : Copepoda : Harpacticoida) expose weaknesses in the conservation of short-range endemics threatened by mining developments in Western Australia." Invertebrate Systematics 27, no. 5 (2013): 540. http://dx.doi.org/10.1071/is12084.

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The discovery of two new non-marine ameirids from the southern Yilgarn region significantly extends the geographic range for this group in Australia and exposes weaknesses in the conservation and environmental impact assessment (EIA) of subterranean species potentially threatened by mining developments. Megastygonitocrella embe, sp. nov. differs from seven previously described Australian congeners by the armature of the second leg endopod and absence of spinules on the somites. A key to world species of Megastygonitocrella is presented. Phylogenetic analysis based on 57 morphological character

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Windsor, Amanda M., and Darryl L. Felder. "Molecular phylogenetics and taxonomic reanalysis of the family Mithracidae MacLeay (Decapoda : Brachyura : Majoidea)." Invertebrate Systematics 28, no. 2 (2014): 145. http://dx.doi.org/10.1071/is13011.

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Mithracid crabs comprise a primarily subtidal reef- and rubble-dwelling group inhabiting both tropical and subtropical seas. Despite their relative ubiquity in many hard-substrate environments, there has been little consensus about their phylogenetic relationships or whether their group rank should be that of subfamily or family. We have used a combined molecular dataset of two nuclear (18S, H3) and three mitochondrial (12S, 16S, COI) genes to build a preliminary molecular phylogeny of Majoidea in order to examine the membership of Mithracidae. We then built a second molecular phylogeny based

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