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Journal articles on the topic 'Islets of Langerhans Islets of Langerhans transplantation'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Lakey, Jonathan R. T., Garth L. Warnock, Ziliang Ao, and Ray V. Rajotte. "Bulk Cryopreservation of Isolated Islets of Langerhans." Cell Transplantation 5, no. 3 (1996): 395–404. http://dx.doi.org/10.1177/096368979600500306.

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Current methods to isolate human islets of Langerhans are limited and multiple donors are required for successful reversal of longstanding Type 1 diabetes mellitus. Cryopreservation of isolated islets is an effective method of storing and pooling islets. Current cryopreservation protocols are cumbersome due to current practices of placing small aliquots of islets per individual freezer tube. In the present study, we examined the application of a blood freezer bag for the cryopreservation of isolated islets by slow cooling and rapid thawing. Freezing and thawing profiles generated using thermoc

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2

Samejima, Tadashi, Kenji Yamaguchi, Hiroo Iwata, Noriyuki Morikawa, and Yoshito Ikada. "Gelatin Density Gradient for Isolation of Islets of Langerhans." Cell Transplantation 7, no. 1 (1998): 37–45. http://dx.doi.org/10.1177/096368979800700106.

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Isolation of islets of Langerhans (islets) has been performed by means of collagenase digestion of the pancreatic tissue combined with density gradient separation of islets from unwanted exocrine tissues. An enormous number of islets are necessary for clinical islet transplantation. The density gradient used for isolation of a large number of islets should satisfy several requirements in addition to those for the conventional density gradients, such as high viscosity for creating fine interfaces with a large area, easy sterilization, and low cost. This study is concerned with the development o

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3

Pakhomov, Oleg, Jiry Honiger, Edouard Gouin, Roland Cariolet, Gerard Reach та Sylviane Darquy. "Insulin Treatment of Mice Recipients Preserves β-Cell Function in Porcine Islet Transplantation". Cell Transplantation 11, № 7 (2002): 721–28. http://dx.doi.org/10.3727/000000002783985422.

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Encapsulation of islets of Langerhans confers protection against cell-mediated immune destruction and so should allow the transplantation of islets without immunosuppression. Xenotransplantation of encapsulated islets of Langerhans might therefore help overcome problems of human organ donor shortage. Given that islets exposed to sustained hyperglycemia show impaired β-cell function, we set out to determine whether recipient treatment with insulin could improve transplantation success rate. Islets of Langerhans were obtained from Specific Germ-Free (SPF) pig pancreas and cultured overnight. Isl

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4

Bucher, Mathe, Bosco, et al. "Islet of Langerhans Transplantation for the Treatment of Type 1 Diabetes." Swiss Surgery 9, no. 5 (2003): 242–46. http://dx.doi.org/10.1024/1023-9332.9.5.242.

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Islet of Langerhans transplantation is gaining recognition as a therapy for type 1 diabetes. The procedure involves enzymatic digestion of the pancreatic tissue, purification of the islets from the exocrine tissue, infusion of the islets into the portal vein and implantation in the liver. Until 1999, an overall rate of insulin independence of 14% at one year was reported in the International Islet Transplant Registry. The results of the "Edmonton protocol" since 2000 were a breakthrough in the field, with reports of 80% insulin independence at 1-year after solitary islet transplantation in non

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5

Gerő, László. "Őssejtterápia, β-sejt- és Langerhans-sziget-neogenezis: a jövő lehetséges terápiái 1-es típusú diabetesben?" Orvosi Hetilap 157, № 19 (2016): 740–45. http://dx.doi.org/10.1556/650.2016.30440.

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In type 1 diabetic patients perfect normoglycaemia can only be achieved by successful transplantation of the pancreas or Langerhans’ islets. Surgical transplantation of the whole pancreas is an invasive operation exerting great burden on the patients. Transplantation of the islets of Langerhans does not burden the patients but the survival of the islet grafts is limited. Both interventions are hampered by the lack of donor organs. However, much of these difficulties could be overcome by the use of “artificial β-cells” which ought to have an ultrastructure identical with that of natural β-cells

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6

Crowther, N. J., C. F. Gotfredsen, A. J. Moody, and I. C. Green. "Immunological and insulin secretory studies on isolated porcine islets of Langerhans." Journal of Endocrinology 126, no. 1 (1990): 43–49. http://dx.doi.org/10.1677/joe.0.1260043.

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ABSTRACT Since porcine islets are considered a likely tissue source for islet transplantation we have studied the insulin secretory responses to stimuli and some of the cell surface antigen characteristics of porcine islet cells. In a static incubation system, the threshold level of glucose required for the stimulation of insulin secretion from freshly isolated porcine islets was found to be between 2·8 and 4·2 mmol glucose/l. Arginine (5 mmol/l) and 3-isobutyl-1-methylxanthine (1 mmol/l) potentiated insulin release induced by 8·3 mmol glucose/l. Leucine (5 mmol/l) initiated release in the pre

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7

Portis, Andrew J., Ray V. Rajotte, and Teresa L. Krukoff. "Reinnervation of Isolated Islets of Langerhans Transplanted beneath the Kidney Capsule in the Rat." Cell Transplantation 3, no. 2 (1994): 163–70. http://dx.doi.org/10.1177/096368979400300204.

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Neural regulation of islets of Langerhans mediates responses to stress and food ingestion. Transplantation of isolated islets offers hope to patients with insulin dependent diabetes mellitus but denervation of isolated islets may affect the capacity for appropriate metabolic control. Previous examination of the endocrine response to stress in islet autografted dogs revealed differences consistent with loss of neural regulation. Therefore, in the present study, islets grafted in rats were examined for extent and nature of reinnervation. Islets isolated from syngeneic donors were grafted under t

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8

Nakagawara, Gizo. "Transplantation of islets of Langerhans." Journal of Hepato-Biliary-Pancreatic Surgery 1, no. 5 (1994): 542–45. http://dx.doi.org/10.1007/bf01211917.

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9

Van Der Burg, Michael P. M., Onno R. Guicherit, Marijke Frölich, Frans A. Prins, Jan Anthonie Bruijn, and Hein G. Gooszen. "Cell Preservation in University of Wisconsin Solution during Isolation of Canine Islets of Langerhans." Cell Transplantation 3, no. 4 (1994): 315–24. http://dx.doi.org/10.1177/096368979400300408.

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Allogeneic islet transplantation in Type I diabetic patients is considerably hampered by the variable outcome of islet isolation and purification. After collagenase digestion of the pancreas, islet isolation is traditionally performed under hypothermic conditions in physiological solutions such as Hanks and RPMI. The University of Wisconsin solution (UWS) has been shown superior for hypothermic preservation of the pancreas. We, therefore, compared the UWS and RPMI for canine islet isolation and subsequent purification in either a conventional hyperosmotic density gradient of dextran in Hanks,

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10

Chao, Seh-Huang, Madhusudan V. Peshwa, David E. R. Sutherland, and Wei-Shou Hu. "Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices." Cell Transplantation 1, no. 1 (1992): 51–60. http://dx.doi.org/10.1177/096368979200100109.

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In vitro culture of islets of Langerhans decreases their immunogenecity, presumably by eliminating passenger leukocytes and other Ia+ presenting cells within the islets. Islets cultivated in petri dishes either at 37°C or at 25°C gradually disintegrate during culture in a time-dependent manner which is related to the free-floating condition of the islets. Also, a fraction of the islets disperse as single cells and beta-cell aggregates or adhere to the bottom of the culture dishes. Thus, the retrieval rate of transplantable islets is dampened due to their disintegration and spontaneous dispersi

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11

Brendel, Mathias D., Shen Shen Kong, Rodolfo Alejandro, and Daniel H. Mintz. "Improved Functional Survival of Human Islets of Langerhans in Three-Dimensional Matrix Culture." Cell Transplantation 3, no. 5 (1994): 427–35. http://dx.doi.org/10.1177/096368979400300510.

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The current study evaluates functional survival of human islets maintained in tissue culture for up to 4 wk in suspension media (CMRL-1066 with supplements) and contrasts these results with immobilizing three-dimensional matrices (agarose or alginate). The absolute number and volume of islets retrieved from agarose is significantly higher after two and four wk of culture compared to conventional free-floating media. In vitro function of islets, assessed by insulin/DNA content, insulin secretion into the culture media over 24 h and glucose-theophylline stimulated insulin release in a dynamic pe

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12

Baranova, N. V., L. A. Kirsanova, Z. Z. Gonikova, A. S. Ponomareva, and V. I. Sevastianov. "Influence of rat bone marrow mesenchymal stem cells to rat Langerhans islets viability during co-cultivation with microst ructured collagen‑containing hydrogel." Russian Journal of Transplantology and Artificial Organs 20, no. 3 (2018): 54–63. http://dx.doi.org/10.15825/1995-1191-2018-3-54-63.

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Aim. To research the rat bone marrow mesenchymal stem cells (MSC BM) effect to the viability of isolated rat OL during cultivation with microstructured collagen-containing hydrogel (BMCH).Materials and methods. Rat islets and MSC BM were isolated according to classical technique with some modifications. The experiments on the co- cultivation were carried out under standard conditions. Islet’s viability was determined by immunofluorescence staining.Results. TheOL cultivation with BMCH and MSCBM resulted in destructive changes of isletson the third day, and complete destruction of OL was observe

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13

Skaletskiy, N. N., L. A. Kirsanova, G. N. Bubentsova, N. V. Baranova, G. N. Skaletskaya, and V. I. Sevastianov. "IDENTIFICATION OF ISLET CAPACITY IN DONOR’S PANCREAS USING IMMUNOMORPHOLOGICAL ANALYSIS." Russian Journal of Transplantology and Artificial Organs 18, no. 1 (2016): 32–37. http://dx.doi.org/10.15825/1995-1191-2016-1-32-37.

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The aimof the work was detailed morphological investigations of donor pancreas (DP) for the study of possibilities of maximal selection of islet tissue suitable for transplantation to a patient of diabetes mellitus type 1.Materials and methods.Eight DPs were received as a result of multiorgan donation. Morphological investigations were performed by means of histological and special immunohistochemical methods.Results.The Majority of islets were revealed in the tail part of the DP. Besides typical Langerhans islets with predominance of mosaically located beta cells, the accumulations of islet c

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14

Zekorn, T. D. C., A. Horcher, J. Mellert, et al. "Biocompatibility and Immunology in the Encapsulation of Islets of Langerhans (Bioartificial Pancreas)." International Journal of Artificial Organs 19, no. 4 (1996): 251–57. http://dx.doi.org/10.1177/039139889601900408.

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Successful transplantation of encapsulated islets (bioartificial pancreas) would circumvent problems of islet availability and rejection in the treatment of insulin-dependent diabetes with biological organ replacement. Alginates are widely used as a hydrogel matrix or membrane for immunoprotected transplantation. A major problem in the use of diffusion-based devices is the biocompatibility of the material used. The foreign body reaction after implantation of empty microcapsules into different compartments in rats, dogs and pigs is evaluated in this article. However, biocompatibility of the bio

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15

Warnock, Garth L., and Jonathan R. T. Lakey. "CRYOPRESERVATION OF HUMAN ISLETS OF LANGERHANS." Transplantation 68, no. 5 (1999): 597–98. http://dx.doi.org/10.1097/00007890-199909150-00001.

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16

DAVIS, D. J., M. A. MACAULAY, A. S. MACDONALD, and B. L. ESTABROOKS. "ISLETS OF LANGERHANS IN DOG PANCREAS." Transplantation 45, no. 6 (1988): 1099–103. http://dx.doi.org/10.1097/00007890-198806000-00020.

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17

Katdare, MR, RR Bhonde, and PB Parab. "Analysis of morphological and functional maturation of neoislets generated in vitro from pancreatic ductal cells and their suitability for islet banking and transplantation." Journal of Endocrinology 182, no. 1 (2004): 105–12. http://dx.doi.org/10.1677/joe.0.1820105.

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The pancreatic ductal stem cells are known to differentiate into islets of Langerhans; however, their yield is limited and the islet population is not defined. Therefore, the aims of the present study were to improvise a methodology for obtaining large numbers of islets in vitro and to characterize their morphological and functional status for islet cell banking and transplantation. Pancreatic ductal epithelial cell cultures were set in serum-free medium. Monolayers of epithelial cells in culture gave rise to islet-like clusters within 3-4 weeks. The identity of neoislets was confirmed by dith

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18

Pathak, Varun, Nupur Madhur Pathak, Christina L. O’Neill, Jasenka Guduric-Fuchs, and Reinhold J. Medina. "Therapies for Type 1 Diabetes: Current Scenario and Future Perspectives." Clinical Medicine Insights: Endocrinology and Diabetes 12 (January 2019): 117955141984452. http://dx.doi.org/10.1177/1179551419844521.

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Type 1 diabetes (T1D) is caused by autoimmune destruction of insulin-producing β cells located in the endocrine pancreas in areas known as islets of Langerhans. The current standard-of-care for T1D is exogenous insulin replacement therapy. Recent developments in this field include the hybrid closed-loop system for regulated insulin delivery and long-acting insulins. Clinical studies on prediction and prevention of diabetes-associated complications have demonstrated the importance of early treatment and glucose control for reducing the risk of developing diabetic complications. Transplantation

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19

Orłowski, T., E. Sitarek, K. Tatarkiewicz, M. Sabat, and M. Antosiak. "Comparison of Two Methods of Pancreas Islets Immunoisolation." International Journal of Artificial Organs 20, no. 12 (1997): 701–3. http://dx.doi.org/10.1177/039139889702001209.

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The efficacy of two methods of Langerhans islets immunoisolation was compared. For this purpose the function of islets encapsulated with alginate/polyethylenimine/protamine/heparin (APPH) or with alginate/poly-L-lisine/alginate (APA) membranes was assessed: in vitro according to their survival and response to glucose challenges, and in vivo according to their capability to provide sufficient insulin delivery to maintain normal fasting blood glucose following xenotransplantation to streptozotocin diabetic mice. In vitro insulin secretion and the response to glucose challenge of APPH and APA enc

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20

Lembert, N., J. Wesche, P. Petersen, et al. "Encapsulation of Islets in Rough Surface, Hydroxymethylated Polysulfone Capillaries Stimulates VEGF Release and Promotes Vascularization after Transplantation." Cell Transplantation 14, no. 2-3 (2005): 97–108. http://dx.doi.org/10.3727/000000005783983232.

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The transplantation of encapsulated islets of Langerhans is one approach to treat type 1 diabetes without the need of lifelong immunosuppression. Capillaries have been used for macroencapsulation because they have a favorable surface-to-volume ratio and because they can be refilled. It is unclear at present whether the outer surface of such capillaries should be smooth to prevent, or rough to promote, cell adhesions. In this study we tested a new capillary made of modified polysulfone (MWCO: 50 kDa) with a rough, open-porous outer surface for islet transplantation. Compared with free-floating

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21

Bertera, Suzanne, Angela M. Alexander, Megan L. Crawford, et al. "Gene Combination Transfer to Block Autoimmune Damage in Transplanted Islets of Langerhans." Experimental Diabesity Research 5, no. 3 (2004): 201–10. http://dx.doi.org/10.1080/15438600490486822.

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Islet transplantation therapy would be applicable to a wider range of diabetic patients if donor islet acceptance and protection were possible without systemic immunosuppression of the recipient. To this aim, gene transfer to isolated donor islets ex vivo is one method that has shown promise. This study examines the combined effect of selected immunomodulatory and anti-inflammatory genes known to extend the functional viability of pancreatic islet grafts in an autoimmune system. These genes, indoleamine 2,3-dioxygenase (IDO), manganese superoxide dismutase (MnSOD), and interleukin (IL)-1 recep

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22

Leibiger, Barbara, Tilo Moede, Ismael Valladolid-Acebes, et al. "Ectopic Leptin Production by Intraocular Pancreatic Islet Organoids Ameliorates the Metabolic Phenotype of ob/ob Mice." Metabolites 11, no. 6 (2021): 387. http://dx.doi.org/10.3390/metabo11060387.

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The pancreatic islets of Langerhans consist of endocrine cells that secrete peptide hormones into the blood circulation in response to metabolic stimuli. When transplanted into the anterior chamber of the eye (ACE), pancreatic islets engraft and maintain morphological features of native islets as well as islet-specific vascularization and innervation patterns. In sufficient amounts, intraocular islets are able to maintain glucose homeostasis in diabetic mice. Islet organoids (pseudo-islets), which are formed by self-reassembly of islet cells following disaggregation and genetic manipulation, b

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23

Clayton, Heather A., and Nick J. M. London. "Survival and Function of Islets during Culture." Cell Transplantation 5, no. 1 (1996): 1–12. http://dx.doi.org/10.1177/096368979600500103.

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Cell and tissue culture techniques have improved considerably since the first attempts to maintain explants of animal tissue in vitro. The two major developments that have allowed these improvements are the ability to produce continuous cell lines, thus allowing reproducible results to be obtained, and the definition of media for different cell types, thereby reducing the need for supplements of serum and other extraneous extracts. The requirements of islets in culture have been more difficult to define, largely because islets do not proliferate in culture and proliferation rate cannot therefo

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24

Hammerman, Marc R. "Engraftment of Insulin-Producing Cells from Porcine Islets in Non-Immune-Suppressed Rats or Nonhuman Primates Transplanted Previously with Embryonic Pig Pancreas." Journal of Transplantation 2011 (2011): 1–7. http://dx.doi.org/10.1155/2011/261352.

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Transplantation therapy for diabetes is limited by unavailability of donor organs and outcomes complicated by immunosuppressive drug toxicity. Xenotransplantation is a strategy to overcome supply problems. Implantation of tissue obtained early during embryogenesis is a way to reduce transplant immunogenicity. Insulin-producing cells originating from embryonic pig pancreas obtained very early following pancreatic primordium formation (embryonic day 28 (E28)) engraft long-term in non-immune, suppressed diabetic rats or rhesus macaques. Morphologically, similar cells originating from adult porcin

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25

Johansson, Ulrika, Annika Olsson, Susanne Gabrielsson, Bo Nilsson, and Olle Korsgren. "Inflammatory mediators expressed in human islets of Langerhans: implications for islet transplantation." Biochemical and Biophysical Research Communications 308, no. 3 (2003): 474–79. http://dx.doi.org/10.1016/s0006-291x(03)01392-5.

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26

Hering, B. J., D. Romann, A. Clarius, et al. "Bovine Islets of Langerhans: Potential Source for Transplantation?" Diabetes 38, Supplement_1 (1989): 206–8. http://dx.doi.org/10.2337/diab.38.1.s206.

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27

Shapiro, A. M. James. "Strategies toward single-donor islets of Langerhans transplantation." Current Opinion in Organ Transplantation 16, no. 6 (2011): 627–31. http://dx.doi.org/10.1097/mot.0b013e32834cfb84.

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28

Wiedemeier, Stefan, Friederike Ehrhart, Esther Mettler, et al. "Encapsulation of Langerhans' islets: Microtechnological developments for transplantation." Engineering in Life Sciences 11, no. 2 (2011): 165–73. http://dx.doi.org/10.1002/elsc.201000146.

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29

Tobin, B. W., Rosemary L. Walzem, Steve M. Watkins, S. K. Leeper-Woodford, Cynthia Bruin, and J. R. T. Lakey. "Lipid Metabolism in Human Pancreatic Islets of Langerhans." Transplantation 76, Supplement (2003): S65. http://dx.doi.org/10.1097/00007890-200308271-00142.

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30

Pehuet-Figoni, Martine, Eric Ballot, Jean-François Bach та Lucienne Chatenoud. "Aberrant Function and Long-Term Survival of Mouse β Cells Exposed In Vitro to High Glucose Concentrations". Cell Transplantation 3, № 5 (1994): 445–51. http://dx.doi.org/10.1177/096368979400300512.

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In vitro culture of murine Langerhans islets usually ends in islet death after 1-3 wk. Given contradictory published data, we studied the influence of glucose on the function and survival of islets from DBA/2 mice. Islets were cultured on plastic microwells, using 1, 2, or 11 g/l glucose concentrations. Using our routine technique, insulin secretion was evaluated after islet incubation for 15 min in basal medium [(bIS), 1 g/1 glucose], followed by 15 min in stimulating medium [(sIS), 3 g/l glucose, 20 mM/l arginine, 5 mM/l theophylline]. Insulin secretion of islets cultured in 1 g/l glucose re

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31

Lakey, J. "Bulk cryopreservation of isolated islets of Langerhans." Cell Transplantation 5, no. 3 (1996): 395–404. http://dx.doi.org/10.1016/0963-6897(95)02025-x.

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32

Klöck, Gerd, Matthias B. Kowalski, Bernhard J. Hering, et al. "Fractions from Commercial Collagenase Preparations: Use in Enzymic Isolation of the Islets of Langerhans from Porcine Pancreas." Cell Transplantation 5, no. 5 (1996): 543–51. http://dx.doi.org/10.1177/096368979600500504.

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Transplantation of isolated islets of Langerhans is an intriguing possibility for the treatment of diabetes mellitus. The isolation of islets from pancreata requires the specific dissociation of the tissue. Commercial collagenases from Clostridium histolyticum are widely used for this purpose. Unfortunately, the effectiveness of these commercial enzymes is not predictable and differs considerably between suppliers and even from lot to lot. This is due mainly to differences in their specific collagenase activity and to the presence of other lytic enzymes, as well as to other contaminants. Free

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33

Pileggi, Antonello, R. Damaris Molano, Thierry Berney, et al. "Prolonged Allogeneic Islet Graft Survival by Protoporphyrins." Cell Transplantation 14, no. 2-3 (2005): 85–96. http://dx.doi.org/10.3727/000000005783983160.

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Transplantation of islets of Langerhans in patients with type 1 diabetes allows for improved metabolic control and insulin independence. The need for chronic immunosuppression limits this procedure to selected patients with brittle diabetes. Definition of therapeutic strategies allowing permanent engraftment without the need for chronic immunosuppression could overcome such limitations. We tested the effect of the use of protoporphyrins (CoPP and FePP), powerful inducers of the cytoprotective protein hemeoxygenase 1 (HO-1), on allogeneic islet graft survival. Chemically induced diabetic C57BL/

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34

Mendola, José F., Ignacio Conget, José María Manzanares, et al. "Follow-up Study of the Revascularization Process of Purified Rat Islet Beta-Cell Grafts." Cell Transplantation 6, no. 6 (1997): 603–12. http://dx.doi.org/10.1177/096368979700600609.

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The revascularization of islets of Langerhans transplanted in heterotopic sites like the liver by portal vein embolization or the renal subcapsular space is a major process necessary for the viability of grafted cells. This process has been extensively studied by different techniques and the results have shown that islet revascularization is an early phenomenon that takes place soon after transplantation. In this report we have analyzed by a double indirect immunofluorescence technique, the revascularization process of purified endocrine islet beta-cells transplanted in the renal subcapsular s

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35

Lacík, Igor. "Polymer Chemistry in Diabetes Treatment by Encapsulated Islets of Langerhans: Review to 2006." Australian Journal of Chemistry 59, no. 8 (2006): 508. http://dx.doi.org/10.1071/ch06197.

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Polymeric materials have been successfully used in numerous medical applications because of their diverse properties. For example, development of a bioartificial pancreas remains a challenge for polymer chemistry. Polymers, as a form of various encapsulation device, have been proposed for designing the semipermeable membrane capable of long-term immunoprotection of transplanted islets of Langerhans, which regulate the blood glucose level in a diabetic patient. This review describes the current situation in the field, discussing aspects of material selection, encapsulation devices, and encapsul

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36

Zagainov, V. Е., A. V. Meleshina, К. G. Korneva, S. А. Vasenin, and E. V. Zagaynova. "Transplantation technologies for treatment of carbohydrate metabolism disorders." Russian Journal of Transplantology and Artificial Organs 22, no. 1 (2020): 184–95. http://dx.doi.org/10.15825/1995-1191-2020-1-184-195.

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The review includes results of retrospective and prospective clinical studies (foreign and national) and guidelines on the use of transplantation technologies for treatment of type 1 diabetes and pancreatogenic diabetes in chronic pancreatitis and pancreatic conditions. Modern data on prevalence of diabetes and modern insulin delivery methods are presented. Results of transplantation of pancreas and islets of Langerhans in primary insulin-dependent conditions are considered. Analysis of the technology for isolation and autotransplantation of islets after pancreatectomy in chronic pancreatitis

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37

Portis, Andrew J., Garth L. Warnock, Diane T. Finegood, Angelo N. Belcastro, and Ray V. Rajotte. "Glucoregulatory response to moderate exercise in long-term islet cell autografted dogs." Canadian Journal of Physiology and Pharmacology 68, no. 10 (1990): 1308–12. http://dx.doi.org/10.1139/y90-196.

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The glucoregulatory response to moderate treadmill exercise ([Formula: see text]60% maximum heart rate; 60 min at 100 m/min, 12% grade) was examined in six controls and six pancreatectomized dogs that had been normoglycemic and insulin independent for more than 1 year since autograft of isolated islets of Langerhans (Tx). There were no significant intergroup differences in plasma glucose levels during exercise, but return to baseline after exercise was delayed in Tx (p < 0.05). In Tx, the initially suppressed insulin levels rose above baseline from 30 to 60 min. Within Tx, exercise-induced

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38

MARCHETTI, PIERO, DAVID W. SCHARP, JOYCE LONGWITH, et al. "PREVENTION OF CONTAMINATION OF ISOLATED PORCINE ISLETS OF LANGERHANS." Transplantation 53, no. 6 (1992): 1364–65. http://dx.doi.org/10.1097/00007890-199206000-00036.

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39

Rafael, E., G. S. Wu, K. Hultenby, A. Tibell, and A. Wernerson. "Improved Survival of Macroencapsulated Islets of Langerhans by Preimplantation of the Immunoisolating Device: A Morphometric Study." Cell Transplantation 12, no. 4 (2003): 407–12. http://dx.doi.org/10.3727/000000003108746957.

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Encapsulation of cells in a semipermeable membrane may in the future provide an opportunity to treat a variety of endocrine and neurological disorders, without the need for lifelong immunosuppression. The physiological conditions in the device are crucial factors for graft survival. Previously, we have shown that the exchange across the immunoisolating membrane and the microcirculation around the TheraCyte™ device increase around 3 months after implantation. The aim of this study was to determine whether preimplantation of the TheraCyte™ device would improve the survival of a later transplante

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Chan, Ka Hei, Rahul Krishnan, Michael Alexander, and Jonathan R. T. Lakey. "Developing a Rapid Algorithm to Enable Rapid Characterization of Alginate Microcapsules." Cell Transplantation 26, no. 5 (2017): 765–72. http://dx.doi.org/10.3727/096368916x693446.

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The islets of Langerhans are endocrine tissue clusters that secrete hormones that regulate the body's glucose, carbohydrate, and fat metabolism, the most important of which is insulin, a hormone secreted by β-cells within the islets. In certain instances, a person's own immune system attacks and destroys them, leading to the development of type 1 diabetes (T1D), a life-long condition that needs daily insulin administration to maintain health and prolong survival. Islet transplantation is a surgical procedure that has demonstrated the ability to normalize blood sugar levels for up to a few year

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Kopska, T., V. Fürstova, and J. Kovar. "Modified Method for Isolation of Langerhans Islets From Mice." Transplantation Proceedings 40, no. 10 (2008): 3611–14. http://dx.doi.org/10.1016/j.transproceed.2008.04.020.

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Burns, Christopher J., Shanta J. Persaud, and Peter M. Jones. "Stem cell therapy for diabetes: do we need to make beta cells?" Journal of Endocrinology 183, no. 3 (2004): 437–43. http://dx.doi.org/10.1677/joe.1.05981.

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Type 1 diabetes can now be ameliorated by islet transplantation, although this treatment is restricted by the insufficient supply of islet tissue. The need for an essentially limitless supply of a substitute for primary human islets of Langerhans has led to research into the suitability of stem/progenitor cells to generate insulin-producing cells to use in replacement therapies for diabetes. Although there has been much research in this area, an efficient and reproducible protocol for the differentiation of stem cells into functional insulin-secreting β-cells that are suitable for transplantat

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Ponomareva, A. S., L. A. Kirsanova, N. V. Baranova, et al. "A technique for separating viable islets of Langerhans from a fragment of human pancreatic tail." Russian Journal of Transplantology and Artificial Organs 20, no. 4 (2019): 76–82. http://dx.doi.org/10.15825/1995-1191-2018-4-76-82.

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Introduction.Modern techniques of tissue engineering in the treatment of some degenerative diseases suggest the prospective viability of the biomedical technologies based on the creation of the equivalent of the damaged tissue (organ), including the tissue-engineered construct (TEC) of the endocrine pancreas (EP). Obtaining viable islets of Langerhans (IL) from the pancreas is a decisive step towards the creation of a TEC EP. The classic method of IL separation is based on enzymatic digestion of pancreatic tissue and further islet purification in ficoll density gradient during centrifugation,

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Salamone, Monica, Salvatrice Rigogliuso, Aldo Nicosia, Simona Campora, Carmelo Marco Bruno, and Giulio Ghersi. "3D Collagen Hydrogel Promotes In Vitro Langerhans Islets Vascularization through ad-MVFs Angiogenic Activity." Biomedicines 9, no. 7 (2021): 739. http://dx.doi.org/10.3390/biomedicines9070739.

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Adipose derived microvascular fragments (ad-MVFs) consist of effective vascularization units able to reassemble into efficient microvascular networks. Because of their content in stem cells and related angiogenic activity, ad-MVFs represent an interesting tool for applications in regenerative medicine. Here we show that gentle dissociation of rat adipose tissue provides a mixture of ad-MVFs with a length distribution ranging from 33–955 μm that are able to maintain their original morphology. The isolated units of ad-MVFs that resulted were able to activate transcriptional switching toward angi

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Jain, Kanti, Hua Yang, Shirin K. Asina, et al. "LONG-TERM PRESERVATION OF ISLETS OF LANGERHANS IN HYDROPHILIC MACROBEADS1." Transplantation 61, no. 4 (1996): 532–36. http://dx.doi.org/10.1097/00007890-199602270-00003.

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WARNOCK, GARTH L., DEREK W. R. GRAY, PHILIP MCSHANE, MAUREEN PETERS, and PETER J. MORRIS. "SURVIVAL OF CRYOPRESERVED ISOLATED ADULT HUMAN PANCREATIC ISLETS OF LANGERHANS." Transplantation 44, no. 1 (1987): 75–82. http://dx.doi.org/10.1097/00007890-198707000-00017.

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Grey, Shane T., Maria B. Arvelo, Wendy Hasenkamp, Fritz H. Bach та Christiane Ferran. "A20 Inhibits Cytokine-Induced Apoptosis and Nuclear Factor κB–Dependent Gene Activation in Islets". Journal of Experimental Medicine 190, № 8 (1999): 1135–46. http://dx.doi.org/10.1084/jem.190.8.1135.

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Insulin-dependent diabetes mellitus (IDDM) is an autoimmune disease resulting from apoptotic destruction of β cells in the islets of Langerhans. Low expression of antioxidants and a predilection to produce nitric oxide (NO) have been shown to underscore β cell apoptosis. With this perspective in mind, we questioned whether β cells could mount an induced protective response to inflammation. Here we show that human and rat islets can be induced to rapidly express the antiapoptotic gene A20 after interleukin (IL)-1β activation. Overexpression of A20 by means of adenovirus-mediated gene transfer p

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Buitinga, Mijke, Roman Truckenmüller, Marten A. Engelse, et al. "Microwell Scaffolds for the Extrahepatic Transplantation of Islets of Langerhans." PLoS ONE 8, no. 5 (2013): e64772. http://dx.doi.org/10.1371/journal.pone.0064772.

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Fraser, Robert B., Malcolm A. Macaulay, James R. Wright, Anthony M. Sun, and Geoffrey Rowden. "Migration of Macrophage-Like Cells within Encapsulated Islets of Langerhans Maintained in Tissue Culture." Cell Transplantation 4, no. 5 (1995): 529–34. http://dx.doi.org/10.1177/096368979500400513.

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Islets of Langerhans isolated from the pancreas and encapsulated in alginate-polylysine-alginate microspheres can potentially serve as a self-regulating supply of insulin in response to glucose loads. A longitudinal ultrastructural and immunohistochemical study of encapsulated rat islets cultured in CMRL-1969 media at a constant glucose concentration of 5.5 mmol/L (100 mg%) allowed several observations. First, acinar cells, which remain attached to isolated islets, disappeared within 1 wk in tissue culture. Damaged endocrine cells also disappeared at this time. Phagocytic cells having ultrastr

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Klaffschenkel, R. A., A. Biesemeier, M. Waidmann, et al. "A Closed System for Islet Isolation and Purification Using the COBE2991 Cell Processor May Reduce the Need of Clean Room Facilities." Cell Transplantation 16, no. 6 (2007): 587–94. http://dx.doi.org/10.3727/000000007783465091.

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During the isolation of human islets of Langerhans the digest has repeated direct contact with the ambient atmosphere. In order to fulfill GMP requirements in clinical applications, the entire cell preparation must be performed in clean room facilities. We hypothesized that the use of a closed system, which avoids the direct exposure of tissue to the atmosphere, would significantly ease the preparation procedure. To avoid the direct atmosphere exposure we tested a modification of the isolation and purification process by performing all islet preparation steps in a closed system. In this study

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