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Journal articles on the topic 'Iso-electric focusing'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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1

Wangoh, J., Z. Farah, and Z. Puhan. "Iso-electric Focusing of Camel Milk Proteins." International Dairy Journal 8, no. 7 (July 1998): 617–21. http://dx.doi.org/10.1016/s0958-6946(98)00092-2.

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2

YOKOHAMA, Michinari, Yasuko WATANABE, and Kazushige MOGI. "A New Equine Transferrin Phenotype Detected by Iso-electric Focusing." Nihon Chikusan Gakkaiho 56, no. 2 (1985): 116–21. http://dx.doi.org/10.2508/chikusan.56.116.

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3

Hawkes, Christopher H., Edward J. Thompson, Geoffrey Keir, John Elston, Mahboub Hawkes, Robert Lamb, and Simon Ruben. "Iso-electric focusing of aqueous humour IgG in multiple sclerosis." Journal of Neurology 241, no. 7 (1994): 436–38. http://dx.doi.org/10.1007/bf00900962.

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4

Menegatti, Marcello, Dino Vaira, John Holton, Fernanda Miranda, Chiara Ricci, Riccardo Gusmaroli, Colin Ainley, Mario Miglioli, and Luigi Barbara. "Serological Response to Helicobacter Pylori in Gastric and Non—Gastric Cancer." Clinical Science 91, no. 2 (August 1, 1996): 219–23. http://dx.doi.org/10.1042/cs0910219.

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1. We aimed to evaluate the seroprevalence of Helicobacter pylori (H. pylori) in gastric cancer, non-gastric cancer and outpatients by ELISA and isoelectric focusing, and to compare histology and serology for H. pylori in gastric cancer and outpatients. 2. In 124 patients with gastric cancer, 78 patients with non-gastric cancer and 110 outpatients, H. pylori seroprevalence was assessed by ELISA and isoelectric focusing. Gastric cancer and outpatients underwent endoscopy with biopsies. 3. Seroprevalence by ELISA was significantly higher in gastric cancer compared with non-gastric cancer (84% ve

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5

Kumar, Mahendra, Bijay P. Tripathi, and Vinod K. Shahi. "Electro-membrane process for the separation of amino acids by iso-electric focusing." Journal of Chemical Technology & Biotechnology 85, no. 5 (May 2010): 648–57. http://dx.doi.org/10.1002/jctb.2348.

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6

Freeman, Amanda N. D., and Peter J. Smith. "Iso‐electric focusing and the identification of fisheries’ waste in the diet of Westland petrels (Procellaria westlandica)." New Zealand Journal of Marine and Freshwater Research 32, no. 2 (June 1998): 177–80. http://dx.doi.org/10.1080/00288330.1998.9516817.

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7

Andersson, Leif, Sven Bengtsson, Ulf Hellman, Ivan Källman та Camilla Ranje. "Genetic variation of haemoglobin α- and βchains in rabbits detected by iso electric focusing and reversed-phase chromatography". Animal Blood Groups and Biochemical Genetics 16, № 1 (24 квітня 2009): 41–50. http://dx.doi.org/10.1111/j.1365-2052.1985.tb01450.x.

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8

Ito, A., A. Plancarte, M. Nakao, K. Nakaya, T. Ikejima, Z. X. Piao, T. Kanazawa, and S. S. Margono. "ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosisin pigs naturally infected with Taenia solium." Journal of Helminthology 73, no. 4 (April 1999): 363–65. http://dx.doi.org/10.1017/s0022149x9900061x.

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The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of preparative iso-electric focusing, which are highly useful for human cysticercosis, have been applied for a serological study in pigs naturally infected withTaenia solium. All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunobl

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9

Loughran, Michael, Shuo-Wen Tsai, Kenji Yokoyama, and Isao Karube. "Simultaneous iso-electric focusing of proteins in a micro-fabricated capillary coated with hydrophobic and hydrophilic plasma polymerized films." Current Applied Physics 3, no. 6 (December 2003): 495–99. http://dx.doi.org/10.1016/j.cap.2003.09.002.

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10

Kourtis, Athena, G. A. Pangalis, L. Borche, Viki A. Boussiotis, G. Dighiero, and Peggy Lymberi. "Monoclonal and/or Oligoclonal Immunoglobulins in Sera of Patients with Non-Hodgkin's Lymphomas, Determined by Iso-Electric Focusing and Immunoblotting." Leukemia & Lymphoma 5, no. 4 (January 1991): 255–62. http://dx.doi.org/10.3109/10428199109068135.

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11

Ciller, U. A., I. M. Ciller, and J. R. McFarlane. "277. Equine chorionic gonadotrophin isoform composition in commercial products compared with isoform composition in pregnant mare plasma." Reproduction, Fertility and Development 20, no. 9 (2008): 77. http://dx.doi.org/10.1071/srb08abs277.

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It is well documented that there is considerable batch to batch variation in the activity of commercial preparations of gonadotrophins. These products are used in a variety of assisted reproductive procedures in the livestock industry, consequently this high degree of variation between products and batches adds to the already considerable between animal variations in response to the treatment. Equine chorionic gonadotrophin (eCG) is a heterodimeric glycoprotein hormone secreted by the placental endometrial cups during the first third of gestation in the horse. Plasma is harvested from pregnant

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12

SOUNART, T. L., and J. C. BAYGENTS. "Lubrication theory for electro-osmotic flow in a non-uniform electrolyte." Journal of Fluid Mechanics 576 (March 28, 2007): 139–72. http://dx.doi.org/10.1017/s0022112006003867.

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A lubrication theory has been developed for the electro-osmotic flow of non-uniform buffers in narrow rectilinear channels. The analysis applies to systems in which the transverse dimensions of the channel are large compared with the Debye screening length of the electrolyte. In contrast with related theories of electrokinetic lubrication, here the streamwise variations of the velocity field stem from, and are nonlinearly coupled to, spatiotemporal variations in the electrolyte composition. Spatially non-uniform buffers are commonly employed in electrophoretic separation and transport schemes,

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13

Bartkowiak, Tomasz, Michał Mendak, Krzysztof Mrozek, and Michał Wieczorowski. "Analysis of Surface Microgeometry Created by Electric Discharge Machining." Materials 13, no. 17 (August 30, 2020): 3830. http://dx.doi.org/10.3390/ma13173830.

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The objective of this work is to study the geometric properties of surface topographies of hot-work tool steel created by electric discharge machining (EDM) using motif and multiscale analysis. The richness of these analyses is tested through calculating the strengths of the correlations between discharge energies and resulting surface characterization parameters, focusing on the most representative surface features—craters, and how they change with scale. Surfaces were created by EDM using estimated energies from 150 to 9468 µJ and measured by focus variation microscope. The measured topograp

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14

Shanahan, P. M. A., C. J. Thomson та S. G. B. Amyes. "β-Lactam resistance in normal faecal flora from South Africa". Epidemiology and Infection 115, № 2 (жовтень 1995): 243–53. http://dx.doi.org/10.1017/s0950268800058374.

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SummaryThe genetic and biochemical basis of ampicillin resistance amongst the aerobic Gram-negative commensal faecal flora of healthy volunteers in South Africa has been determined. Amongst 608 ampicillin resistant strains isolated from 320 of the participants, 158 were able to transfer their ampicillin resistant determinants intoEscheriehia coliK-12 J62–2. Iso-electric focusing of the β-lactamases, extracted from the transconjugants, demonstrated that ampicillin resistance resulted from the presence of the TEM-1, TEM-2 and SHV-1 β-lactamases in 94·3%, 2·5% and 3·2% of isolates respectively. E

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15

Siew, Ye Wee, Karina Listiarini Zedda, and Svetlozar Velizarov. "Nanofiltration of Simulated Acid Mine Drainage: Effect of pH and Membrane Charge." Applied Sciences 10, no. 1 (January 5, 2020): 400. http://dx.doi.org/10.3390/app10010400.

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Acid mine drainage (AMD) is a severe form of environmental pollution that has the potential to contaminate surface and ground waters by introducing heavy metals and lowering the pH. The feasibility of using nanofiltration (NF) as a potentially attractive and cost-effective remediation method to treat acid mine drainage was investigated in this study. The performance of an acid-stable NF membrane focusing on the effects of the water pH and membrane charge on ion rejection was systematically studied. A single salt solution experiment showed that Mg, Cu, and Mn containing species were highly reje

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16

Streetly, Allison, Mary Clarke, Melanie Downing, Lisa Farrar, Ying Foo, Kate Hall, Helena Kemp, et al. "Implementation of the newborn screening programme for sickle cell disease in England: results for 2003-2005." Journal of Medical Screening 15, no. 1 (March 2008): 9–13. http://dx.doi.org/10.1258/jms.2008.007063.

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Objectives This paper reports early screening results from the newborn sickle cell disease screening programme recently implemented in England. Setting England. Screening is offered at 5-8 days of age as part of the existing bloodspot test and offered to all babies irrespective of ethnicity. Methods The laboratory methods recommended are high performance liquid chromatography (HPLC) and iso-electric focusing (IEF). 15 Two methods of analysis must be applied to all screen positive results. The conditions screened for are:- Sickle cell anaemia (Hb SS), Hb SC disease, Hb S/β-thalassaemia, Hb S/DP

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17

Ciller, U. A., J. D. McFarlane, and J. R. McFarlane. "512. COMMERCIAL EQUINE CHORIONIC GONADOTROPHIN ISOFORM COMPOSITION, IMMUNOACTIVITY, AND BIOACTIVITY IN A MURINE MODEL USING OVARIAN AUGMENTATION AND TESTICULAR INTERSTITIAL CELL BIOASSAYS." Reproduction, Fertility and Development 21, no. 9 (2009): 111. http://dx.doi.org/10.1071/srb09abs512.

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Equine chorionic gonadotrophin (eCG) is a placental glycoprotein hormone that is harvested from the plasma of pregnant mares for the formulation of commercial products which are used in a variety of assisted reproductive procedures in livestock. It is well documented that the bioactivity of gonadotrophin products is highly variable. The aim of this study was therefore to determine how different eCG products affected target tissues and cells. Isoforms of eCG were separated with iso-electric focusing and immunoactivity measured with RIA. For in vivo bioassay, dose-response eCG treatments were ad

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18

Wepner, Lena, Harald Andreas Färber, Andreas Jaensch, Anna Weber, Florian Heuser, Ludger Keilig, Lamia Singer, and Christoph Peter Bourauel. "In Vitro Ion Release of Wires in Removable Orthodontic Appliances." Materials 14, no. 12 (June 19, 2021): 3402. http://dx.doi.org/10.3390/ma14123402.

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Various orthodontic wire compositions and configurations are present on the market for removable appliances; however, there have still been only few studies focusing on the effect of resin color and additives such as glitter on corrosion of metallic wires under different conditions. Thus, the aim of the study was to compare concentrations of released ions (aluminium, chromium, nickel) in a corrosive medium under three different conditions: non-loaded wires, loaded wires, and non-loaded wires treated with Kukis® cleaning tablets. Six different wires made of three types of steel alloy were embed

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19

Forrest, Richard D., Caroline A. Jackson, Barry J. Gould, Marianne Casburn-Budd, Julie E. Taylor, and John S. Yudkin. "Short-Term Effect of a 75 g Oral Glucose Load on Glycohaemoglobin Levels." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 24, no. 1 (January 1987): 53–57. http://dx.doi.org/10.1177/000456328702400108.

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Two hundred and twenty-three subjects out of a total of 347 with various degrees of glucose tolerance were recalled after a screening survey for diabetes. They were a randomly selected sample of people over the age of 40 and they underwent a formal 75 g glucose tolerance test in order to assess the effect of a glucose load on glycohaemoglobin levels measured by four different assay methods. Oral glucose loading was found to affect glycohaemoglobin levels only when these were measured by an agar-gel electrophoretic method that did not remove the labile aldimine-linked Schiff base fraction. The

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20

Chunda-Liyoka, Catherine Mupela, Ashok A. Kumar, Pauline M. Sambo, Felicity Lubinda, Tyler Humpton, Pauline Okuku, Chimuka Miyanda, et al. "Successful Field Test of a Combined Health Access Strategy and Novel Device to Screen for Sickle Cell Disease (SCD) in Rural Sub-Saharan Africa." Blood 132, Supplement 1 (November 29, 2018): 316. http://dx.doi.org/10.1182/blood-2018-99-114625.

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Abstract Despite the fact that simple interventions and emerging therapies can increase survival of SCD patients, under detection renders SCD a major cause of under age-5 mortality in rural regions of the developing world where it is prevalent. (Piel et PLoS Med DOI: org/10.1371/journal.pmed.1001484, 2013). Two major diagnostic challenges are: 1. A lack of affordable diagnostic methodology to obtain point-of-care results in low-resource environments. 2. Dispersion of SCD patients in vast rural hinterlands who rarely interface with healthcare services. To address the first impediment, we have d

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21

Linssen, Matthijs David, Wouter Tjerk Rudolph Hooghiemstra, Annelies Jorritsma-Smit, Derk Pieter Allersma, Gerard Dijkstra, and Wouter Bastiaan Nagengast. "Development and Characterisation of Antibody-Based Optical Imaging Probes for Inflammatory Bowel Disease." Pharmaceuticals 14, no. 9 (September 13, 2021): 922. http://dx.doi.org/10.3390/ph14090922.

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Monoclonal antibodies are an important addition to the medicinal treatment paradigm for IBD patients. While effective, these agents show a high degree of primary and secondary non-response, and methods to predict response are highly desired. Information on drug distribution at the target level is often lacking. Fluorescent endoscopic imaging using labelled antibody drugs may provide insight regarding drug distribution, target engagement and drug response, but these assessments require stable and functional fluorescently-conjugated probes. Infliximab, vedolizumab, adalimumab and ustekinumab wer

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