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Relevant bibliographies by topics / Isocratic-HPLC

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Academic literature on the topic 'Isocratic-HPLC'

Author: Grafiati

Published: 4 June 2025

Last updated: 6 July 2025

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Journal articles on the topic "Isocratic-HPLC"

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Rosano, T. G., R. T. Ambrose, A. H. Wu, T. A. Swift, and P. Yadegari. "Candidate reference method for determining creatinine in serum: method development and interlaboratory validation." Clinical Chemistry 36, no. 11 (1990): 1951–55. http://dx.doi.org/10.1093/clinchem/36.11.1951.

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Abstract We describe a "high-performance" liquid chromatographic (HPLC) method for accurately determining creatinine in serum. After prechromatographic precipitation of protein, we performed isocratic ion-exchange chromatography with ultraviolet detection (234 nm). Analytical results showed linearity up to 1770 mumol/L, a detection limit of 22 mumol/L, an average analytical recovery of 101%, and a CV ranging from 3% to 11%. We used certified human serum (National Institute of Standards and Technology), and additional lyophilized serum pools also assayed by definitive isotope-dilution mass spectrometry, to validate the accuracy of the HPLC method. In addition, the isocratic HPLC results showed close agreement with those obtained with a step-gradient HPLC method. We also compared the isocratic HPLC method with alkaline picrate and enzymatic methods. Our findings with samples from nonuremic, uremic, and diabetic ketoacidotic patients confirmed the positive bias previously reported with the alkaline picrate method. Interlaboratory transferability of the method was demonstrated with various commercial instruments and analytical columns. We evaluated column stability and possible interference from endogenous or exogenous compounds. On the basis of our analytical findings, we recommend the isocratic HPLC method as a candidate Reference Method for determining creatinine in serum.

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Sirvent, Tara, and Donna M. Gibson. "RAPID ISOCRATIC HPLC ANALYSIS OF HYPERICINS." Journal of Liquid Chromatography & Related Technologies 23, no. 2 (2000): 251–59. http://dx.doi.org/10.1081/jlc-100101449.

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Jebaliya, Hetal, Madhavi Patel, Yashwant Jadeja, Batuk Dabhi, and Anamik Shah. "A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study." Chromatography Research International 2013 (December 4, 2013): 1–5. http://dx.doi.org/10.1155/2013/673150.

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The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 (250 × 4.5 mm, 5 μm particle size) column has been used for HPLC and BEH C18 (100 × 2.1 mm, 1.7 μm particle size) column used for UPLC. The Mobile phase consisted of Methanol : Water (70 : 30) for HPLC and Methanol : Water (55 : 45 v/v) for UPLC. Isocratic flow was set at 1 mL/min and 0.30 mL/min, respectively, for HPLC and UPLC. For both HPLC and UPLC system detection has been performed at 211 nm with 30°C column oven temperature (good elution was obtained at 30°C) and injection volume, respectively, 2 μL and 20 μL for HPLC and UPLC.

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Krauss, G. J., V. Leinhos, and K. Glund. "Highly specific isocratic HPLC of UDP-glucose." Fresenius' Zeitschrift für analytische Chemie 324, no. 3-4 (1986): 337. http://dx.doi.org/10.1007/bf00487974.

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Abushufa, R., P. Reed, and C. Weinkove. "Fatty acids in erythrocytes measured by isocratic HPLC." Clinical Chemistry 40, no. 9 (1994): 1707–12. http://dx.doi.org/10.1093/clinchem/40.9.1707.

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Abstract We developed an isocratic reversed-phase HPLC method to measure arachidonic, palmitoleic, linoleic, eicosatrienoic, oleic, palmitic, and stearic acids from hydrolyzed erythrocytes. Washed erythrocytes were heated in methanol:HCl and the fatty acids extracted into hexane:amyl alcohol. After derivatization with 4-bromomethyl-7-methoxycoumarin, samples diluted in mobile phase (acetonitrile:water, 85:15 by vol) were injected onto a 250 x 4.6 mm C18 column, and the eluted fatty acids were detected fluorometrically. For all analytes, the mean within-batch CV was 8.2% (5.5-10.8%), the mean limit of detection was 7.0 mumol/L, a linear response was maintained up to 400 mumol/L, and results agreed well with those by gas chromatography. The addition of antioxidant (butylated hydroxytoluene) was essential for sample stability. We discuss hydrolysis and extraction times, derivatization temperature, critical steps in chromatography, and concentration units.

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Alarcón, P., A. Bustos, B. Cañas, M. D. Andrés, and L. M. Polo. "Determination of priority pollutant phenols by isocratic HPLC." Chromatographia 24, no. 1 (1987): 613–16. http://dx.doi.org/10.1007/bf02688553.

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Dr., S. Naazneen. "A Validated Isocratic RP-HPLC Method for the Quantification of Vericiguat in Solid Dosage Forms." Journal of Pharma Research 13, no. 03 (2024): 17–22. https://doi.org/10.5281/zenodo.11400501.

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<strong><em>ABSTRACT</em></strong> <strong><em> </em></strong><strong>An accurate, sensitive, precise, quick isocratic reverse phase HPLC (RP-HPLC) technique has been developed and validated for the quantification of Vericiguat in the solid dosage forms.  The best separation was achieved on a 250 mm x 4.6 mm ID., 5µ-particle size Xterra®-Octadecyl-Silyl-3V-Reverse-Phase-C18-column with 0.03M KH2PO4 in water: acetonitrile (30:70 v/ v) with pH-3.2 in the isocratic mode of elution as mobile phase solvent at a speed of 0.5 ml/ min. UV detection was at 218 nm. Retention time of Vericiguat was found to be 5.8 minutes. With a correlation coefficient of about 0.998, peak-response was obtained as function of concentration over the range of 29.6 to 88.8 µg/ ml for Vericiguat. Vericiguat had shown to have a percentage assay of 109.73 %. Vericiguat had a limit of detection and a limit of quantification (LOQ) of 0.074 µg/ ml and 0.222 µg/ ml, respectively. The presence of excipients in the formulation had no effect on the assay method. The procedure is appropriate for use in QC- laboratories since it is quick and precise</strong> <strong>Keywords: Vericiguat, Isocratic-RP-HPLC, solid dosage forms</strong>

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Whitmore, W. L., and T. A. Slotkin. "A simplified method for isocratic HPLC analysis of polyamines." Experientia 41, no. 9 (1985): 1209–11. http://dx.doi.org/10.1007/bf01951733.

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Walker, Gregory S., Michael F. Coveney, Michael J. Klug, and Robert G. Wetzel. "Isocratic HPLC analysis of adenine nucleotides in environmental samples." Journal of Microbiological Methods 5, no. 5-6 (1986): 255–64. http://dx.doi.org/10.1016/0167-7012(86)90050-3.

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Al-Sinani, Sana S., and Elsadig A. Eltayeb. "Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography." Sultan Qaboos University Journal for Science [SQUJS] 18 (December 1, 2013): 1. http://dx.doi.org/10.24200/squjs.vol18iss0pp1-10.

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Improved and simplified HPLC conditions for the determination and quantification of both the steroidal glycoalkaloid (solamargine) and its aglycone (solasodine) are described. The best isocratic conditions were developed using a C18 column and methanol in combination with an ammonium dihydrogen phosphate buffer. The isocratic conditions were shown to be more reproducible, less time consuming and to give sharper peaks (better separation). The effects of adjusting solvent: buffer ratio, buffer pH and buffer molarity were evaluated.

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Dissertations / Theses on the topic "Isocratic-HPLC"

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Kim, Tae-Young. "Novel sol-gel titania-based hybrid organic-inorganic coatings for on-line capillary microextraction coupled to high-performance liquid chromatography." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001833.

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Book chapters on the topic "Isocratic-HPLC"

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Mattaliano, R. J., K. A. Yamada, J. K. Hughes, and P. M. Yuan. "Design and Performance Features of a Simplified Protein Sequencer with an On-Board Isocratic HPLC System." In Methods in Protein Sequence Analysis. Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73834-0_14.

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Frank, Gerhard. "New Aspects in Isocratic HPLC Separation of Phenylthiohydantoin Amino Acids Through the Application of Ionic Detergents." In Methods in Protein Sequence Analysis. Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73834-0_15.

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Shively, J. E., D. Hawke, R. M. Kutny, B. Krieger, and J. L. Glajch. "An On-Line Isocratic HPLC System for the Analysis of PTH-Amino Acids on A Gas-Phase Sequencer." In Proteins. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-1787-6_38.

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Lazarus, L. H., and O. Hernandez. "Physalaemin-Like Immunoreactivity from Human Lung Small Cell Carcinoma: Isocratic Reversed-Phase HPLC Analysis of the Chemically Modified Peptide." In Peptide Hormones in Lung Cancer. Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-82533-0_6.

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Jandera, Pavel. "Isocratic HPLC." In Encyclopedia of Chromatography, Third Edition (Print Version). CRC Press, 2009. http://dx.doi.org/10.1201/noe1420084597.ch251.

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Jandera, Pavel. "Isocratic HPLC." In Encyclopedia of Chromatography, Second Edition. CRC Press, 2005. http://dx.doi.org/10.1201/noe0824727857.ch191.

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Jandera, Pavel. "Isocratic HPLC: Selection of a System." In Encyclopedia of Chromatography. CRC Press, 2005. http://dx.doi.org/10.1201/noe0824727857-191.

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Godela, Ramreddy, Yagnambhatla Rajendra, Kranthi Kumar Pola, Dr Beda Durgaprasad, and Rani Dumpala. "IMPLEMENTATION OF GREEN SOLVENT SYSTEM FOR THE ANALYSIS OF ADAPALENE IN BULK AND TOPICAL GEL FORMULATION BY RP-HPLC." In Futuristic Trends in Pharmacy & Nursing Volume 2 Book 25. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2023. http://dx.doi.org/10.58532/v2bs25p2ch3.

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A simple and rapid stabilityindicating, RP-HPLC method was ascertained to determine adapalene in bulk and gel formulation. Separation was achieved on enable C18 column (150 x 4.6mm; 5μm) under the isocratic mode of elution by using mobile system mixture of green solvents tetrahydrofuran and methanol (30:70 V/V). The flow rate was maintained at 1.0 ml/min with runtime 10min. UV detection was done at 360 nm. The method was found to be linear for series concentration ranges from 20-100µg/ml. The limit of detection and quantification were found to be 3.27and 0.025µg/ml. Results of precision and accuracy obtained were within the limits. The suggested approach was effectively employed for the determination of adapalene in a labelled formulation (gel), with a % assay of 99%. The suggested method's stability-indicating capacity is shown by evaluating forced degradation samples in which the peak purity of adapalene is determined as well as the resolution of degradant from the analyte peak. The collected findings demonstrate that the provided approach is a stability indicating method. The stated HPLC method has been verified in terms of specificity, precision, sensitivity, accuracy, linearity, and robustness according to ICH

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Mohammed, Jahasultana, and Buchi N. Nalluri. "Development and Validation of RP-HPLC-PDA Method for the Estimation of Fluoxetine Hydrochloride in Bulk, Mouth Dissolving Films and in Dissolution Samples." In Current Trends in Drug Discovery, Development and Delivery (CTD4-2022). Royal Society of Chemistry, 2023. http://dx.doi.org/10.1039/9781837671090-00283.

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Selective serotonin reuptake inhibitor, fluoxetine hydrochloride (FLX) is a drug of choice for treating depression, obsessive-compulsive disorder (OCD), bulimia nervosa, etc. Even though FLX dosage forms such as tablets, capsules, oral solutions, and syrups are already available commercially, there is a considerable need for developing modified or immediate-release formulations with a quick onset of action and high bioavailability. The goal of this research is to provide a quick, practical, and cost-effective RP-HPLC-PDA approach for determining Fluoxetine hydrochloride (FLX) in bulk, mouth dissolving films (MDFs), and dissolution samples. A reverse-phase HPLC with chromatographic parameters like inertsil ODS3 column (150×4.6mm, 5µm), mobile phase composition of 10mM Ammonium acetate: Acetonitrile (58:42v/v), flow rate - 1.3mL/min, 20 μL injection volume in isocratic mode, wavelength at 226nm and a PDA detector is used to attain the peaks. Using the efficient liquid chromatographic conditions, FLX was eluted at retention time of 4.2min and a good linearity was seen over a concentration of 2-10µg/mL. The correlation coefficient (R2) was 0.999. The LOD and LOQ were 0.184μg/mL and 0.558μg/mL, respectively. The established technique was validated for method validation parameters like accuracy, precision, assay, linearity, specificity, robustness, LOD, and LOQ as per ICH Q2B recommendations. All the validation parameters were within acceptable limits. Hence, for the FLX estimation in bulk, MDFs and in-vitro dissolution samples the established technique can be utilized to produce reproducible results.

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Kalyani, Potu, Buchi N. Nalluri, M. Chinna Eswaraiahc, and Tata Prasanna Kumarib. "Development of RP-HPLC-PDA Method Validation for the Simultaneous Estimation of Zidovudine, Lamivudine, and Nevirapine in Bulk and Dosage Forms and in Dissolution Samples." In Current Trends in Drug Discovery, Development and Delivery (CTD4-2022). Royal Society of Chemistry, 2023. http://dx.doi.org/10.1039/9781837671090-00316.

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The purpose of this research is to design a rapid, efficient, and cost-effective RP-HPLC-PDA for the simultaneous determination of Nevirapine (NVP), Lamivudine (LMV), and Zidovudine (ZDV) in bulk, dosage forms, and dissolving samples. The maintained chromatographic conditions were an Inertsil ODS3 column (150×4.6mm,5µm) with 15mM Ammonium acetate: Methanol (55:45 v/v) as a mobile phase, injected in isocratic mode at flow-rate 1mL/min, eluents were measured at 270nm. LMV, ZDV, and NVP were eluted at 2.24, 3.88, and 8.15 minutes, respectively, under these LC conditions. At concentrations ranging from 5 to 25µg/mL, LMV (R2 = 0.999), ZDV (R2 = 0.997), and NVP (R2 = 0.996) exhibited excellent linearity. The LOD and LOQ for LMV were 0.296µg/mL and 0.899μg/mL, respectively, for ZDV were 0.105μg/mL, 0.320μg/mL and NVP were 0.113μg/mL, 0.343μg/mL respectively. In every instance, the validation acceptance criteria for variables such as accuracy, precision, assay, linearity, specificity, and robustness were met. The validated approach was utilized well for estimation of LMV, ZDV, and NVP in bulk, dosage forms, and dissolution samples.

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Conference papers on the topic "Isocratic-HPLC"

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Martono, Yohanes, Sugeng Riyanto, Abdul Rohman, and Sudibyo Martono. "Improvement method of fast and isocratic RP-HPLC analysis of major diterpene glycoside from Stevia rebaudiana leaves." In PROCEEDINGS OF THE 12TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION – SRI2015. Author(s), 2016. http://dx.doi.org/10.1063/1.4958509.

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Cojocaru-Toma, Maria, Vladimir Valica, Vladilena Gandacov, Ecaterina Mazur, and Livia Uncu. "Phenolic compounds in Agrimonia eupatoria l. Ethanolic extract evaluated by HPLC." In Scientific seminar with international participation "New frontiers in natural product chemistry". Institute of Chemistry, Republic of Moldova, 2023. http://dx.doi.org/10.19261/nfnpc.2023.ab17.

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Agrimonia eupatoria L. (Rosaceae fam.) is widespread in temperate regions. In folk medicine, this species has been used for its astringent, analgesic and anti-inflammatory, as well as in gastrointestinal disorders. As these biological properties have been linked to its phenolic composition, this plant species could be an interesting source of bioactive compounds with therapeutic potential [1]. For the evaluation of chemical compounds in A. eupatoria extract, the aerial parts were harvested in the flowering period from the collection of the Scientific Practical Centre in the Field of Medicinal Plants of “Nicolae Testemițanu” State University of Medicine and Pharmacy. The dry extracts were concentrated using a rotary evaporator-Laborota 4011. The analysis was performed on Shimadzu LC-20AD chromatograph with SPD-20A UV detector [2], under the following conditions: stationary phase - Zorbax Exlipse Plus C18 (4.6x250 mm, 5 microns); 2 mobile phases: solvent mixture: methanol: water (40:60) with gradient elution and orthophosphoric acid 0.5%: acetonitrile (80:20) with isocratic elution mode; detection at wavelengths of 280, 325 and 360 nm. The solvent system that achieved optimal separation of the phenolic compounds was the mixture of 0.5% orthophosphoric acid:acetonitrile (80:20) at a wavelength of 325 nm. The extract of A. eupatoria was found to be richer in tannins (catechin-3.68%, epicatechin-2.59%); in flavonoids (rutin-1.71%, quercetin-0.199%, luteolin-0.184%, kaempferol-0.162%, apigenin-0.078%, with a lower content of hydroxycinnamic acids (chlorogenic, caffeic, cycoric and ellagic). The HPLC method with UV-VIS detection can be successfully used in the identification and quantitative determination of chemical compounds in Agrimonia eupatoria extracts for the development and standardization of new pharmaceutical forms.

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