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Journal articles on the topic 'Isocratic-HPLC'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Rosano, T. G., R. T. Ambrose, A. H. Wu, T. A. Swift, and P. Yadegari. "Candidate reference method for determining creatinine in serum: method development and interlaboratory validation." Clinical Chemistry 36, no. 11 (1990): 1951–55. http://dx.doi.org/10.1093/clinchem/36.11.1951.

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Abstract We describe a "high-performance" liquid chromatographic (HPLC) method for accurately determining creatinine in serum. After prechromatographic precipitation of protein, we performed isocratic ion-exchange chromatography with ultraviolet detection (234 nm). Analytical results showed linearity up to 1770 mumol/L, a detection limit of 22 mumol/L, an average analytical recovery of 101%, and a CV ranging from 3% to 11%. We used certified human serum (National Institute of Standards and Technology), and additional lyophilized serum pools also assayed by definitive isotope-dilution mass spec

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2

Sirvent, Tara, and Donna M. Gibson. "RAPID ISOCRATIC HPLC ANALYSIS OF HYPERICINS." Journal of Liquid Chromatography & Related Technologies 23, no. 2 (2000): 251–59. http://dx.doi.org/10.1081/jlc-100101449.

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3

Jebaliya, Hetal, Madhavi Patel, Yashwant Jadeja, Batuk Dabhi, and Anamik Shah. "A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study." Chromatography Research International 2013 (December 4, 2013): 1–5. http://dx.doi.org/10.1155/2013/673150.

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The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 (250 × 4.5 mm, 5 μm particle size) column has been used for HPLC and BEH C18 (100 × 2.1 mm, 1.7 μm particle size) column used for UPLC. The Mobile phase consisted of Methanol : Water (70 : 30) for HPLC and Methanol : Water (55 : 45 v/v) for UPLC. Isocratic flow was set at 1 mL/min and 0.30 mL/min, respectively, for HPLC and UPLC. For both HPLC and UPLC system detection has been performe

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4

Krauss, G. J., V. Leinhos, and K. Glund. "Highly specific isocratic HPLC of UDP-glucose." Fresenius' Zeitschrift für analytische Chemie 324, no. 3-4 (1986): 337. http://dx.doi.org/10.1007/bf00487974.

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5

Abushufa, R., P. Reed, and C. Weinkove. "Fatty acids in erythrocytes measured by isocratic HPLC." Clinical Chemistry 40, no. 9 (1994): 1707–12. http://dx.doi.org/10.1093/clinchem/40.9.1707.

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Abstract We developed an isocratic reversed-phase HPLC method to measure arachidonic, palmitoleic, linoleic, eicosatrienoic, oleic, palmitic, and stearic acids from hydrolyzed erythrocytes. Washed erythrocytes were heated in methanol:HCl and the fatty acids extracted into hexane:amyl alcohol. After derivatization with 4-bromomethyl-7-methoxycoumarin, samples diluted in mobile phase (acetonitrile:water, 85:15 by vol) were injected onto a 250 x 4.6 mm C18 column, and the eluted fatty acids were detected fluorometrically. For all analytes, the mean within-batch CV was 8.2% (5.5-10.8%), the mean l

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6

Alarcón, P., A. Bustos, B. Cañas, M. D. Andrés, and L. M. Polo. "Determination of priority pollutant phenols by isocratic HPLC." Chromatographia 24, no. 1 (1987): 613–16. http://dx.doi.org/10.1007/bf02688553.

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7

Dr., S. Naazneen. "A Validated Isocratic RP-HPLC Method for the Quantification of Vericiguat in Solid Dosage Forms." Journal of Pharma Research 13, no. 03 (2024): 17–22. https://doi.org/10.5281/zenodo.11400501.

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<strong><em>ABSTRACT</em></strong> <strong><em> </em></strong><strong>An accurate, sensitive, precise, quick isocratic reverse phase HPLC (RP-HPLC) technique has been developed and validated for the quantification of Vericiguat in the solid dosage forms.  The best separation was achieved on a 250 mm x 4.6 mm ID., 5µ-particle size Xterra®-Octadecyl-Silyl-3V-Reverse-Phase-C18-column with 0.03M KH2PO4 in water: acetonitrile (30:70 v/ v) with pH-3.2 in the isocratic mode of elution as mobile phase solvent at a speed of 0.5 ml/ min. UV detection was at 218 nm. Retention time of

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8

Whitmore, W. L., and T. A. Slotkin. "A simplified method for isocratic HPLC analysis of polyamines." Experientia 41, no. 9 (1985): 1209–11. http://dx.doi.org/10.1007/bf01951733.

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9

Walker, Gregory S., Michael F. Coveney, Michael J. Klug, and Robert G. Wetzel. "Isocratic HPLC analysis of adenine nucleotides in environmental samples." Journal of Microbiological Methods 5, no. 5-6 (1986): 255–64. http://dx.doi.org/10.1016/0167-7012(86)90050-3.

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10

Al-Sinani, Sana S., and Elsadig A. Eltayeb. "Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography." Sultan Qaboos University Journal for Science [SQUJS] 18 (December 1, 2013): 1. http://dx.doi.org/10.24200/squjs.vol18iss0pp1-10.

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Improved and simplified HPLC conditions for the determination and quantification of both the steroidal glycoalkaloid (solamargine) and its aglycone (solasodine) are described. The best isocratic conditions were developed using a C18 column and methanol in combination with an ammonium dihydrogen phosphate buffer. The isocratic conditions were shown to be more reproducible, less time consuming and to give sharper peaks (better separation). The effects of adjusting solvent: buffer ratio, buffer pH and buffer molarity were evaluated.

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11

Abdulla, Shabaan A., Eman Y. Frag, and Heba E. Ahmed. "Kinetic study of the alkaline degradation of imidapril hydrochloride using a validated stability indicating HPLC method." RSC Advances 6, no. 73 (2016): 69239–50. http://dx.doi.org/10.1039/c6ra11953k.

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An aqueous alkaline degradation study was performed for imidapril hydrochloride (IMD) drug in the presence of its degradation products and an isocratic stability indicating method was presented using HPLC.

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12

Holland, Brendan J., Xavier A. Conlan, Paul S. Francis, Neil W. Barnett, and Paul G. Stevenson. "Overcoming solvent mismatch limitations in 2D-HPLC with temperature programming of isocratic mobile phases." Analytical Methods 8, no. 6 (2016): 1293–98. http://dx.doi.org/10.1039/c5ay02528a.

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Solvent incompatibility is a limiting factor when implementing 2D-HPLC. A programmed temperature gradient in the first dimension was developed to overcome this limitation by allowing an isocratic separation while maintaining selectivity.

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13

Ibrahim, Fawzia, Mohie Khaled Sharaf El-Din, Rania Nabih El-Shaheny, Asmaa Kamal El-Deen, and Kuniyoshi Shimizu. "Simultaneous determination of four vasoactive phytochemicals in different pharmaceutical preparations by a simple HPLC-DAD method." Analytical Methods 8, no. 8 (2016): 1858–66. http://dx.doi.org/10.1039/c5ay03003j.

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A new, simple isocratic HPLC method was developed and validated for the simultaneous estimation of four vasoactive phytochemicals: ascorbic acid (ASC), rutin (RUT), hesperidin (HSP), and diosmin (DSM) in different pharmaceutical preparations.

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14

Horník, Štěpán, Marie Sajfrtová, Jindřich Karban, Jan Sýkora, Anna Březinová, and Zdeněk Wimmer. "LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts." Journal of Analytical Methods in Chemistry 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/526818.

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The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.

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15

Ranjbarian, Farahnaz, Sushma Sharma, Giulia Falappa, Walter Taruschio, Andrei Chabes, and Anders Hofer. "Isocratic HPLC analysis for the simultaneous determination of dNTPs, rNTPs and ADP in biological samples." Nucleic Acids Research 50, no. 3 (2021): e18-e18. http://dx.doi.org/10.1093/nar/gkab1117.

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Abstract Information about the cellular concentrations of deoxyribonucleoside triphosphates (dNTPs) is instrumental for mechanistic studies of DNA replication and for understanding diseases caused by defects in dNTP metabolism. The dNTPs are measured by methods based on either HPLC or DNA polymerization. An advantage with the HPLC-based techniques is that the parallel analysis of ribonucleoside triphosphates (rNTPs) can serve as an internal quality control of nucleotide integrity and extraction efficiency. We have developed a Freon-free trichloroacetic acid-based method to extract cellular nuc

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16

Naveed, Safila. "FACILE AND MANIFEST RPHPLC METHOD FOR THE DETERMINATION OF CAPTPRIL, LISIOPRIL, ENALPRIL AND DICLOFENAC SODIUM: ITS APPLICATIONS IN DOSAGE FORMULATIONS AND IN HUMAN SERUM." Canadian Journal of Applied Sciences 4 (2014): 40. http://dx.doi.org/10.21065/19257430.40.4.

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A simple, rapid, isocratic, high-performance liquid chromatography (RP-HPLC) method has been developed for the first time for simultaneous determination of ACE inhibitors (captopril, lisinopril and enalapril) and diclofenac sodium in bulk drugs, pharmaceutical products and human serum.

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17

Ulusoy, Songül, Halil Ibrahim Ulusoy, Daniel Pleissner, and Niels Thomas Eriksen. "Nitrosation and analysis of amino acid derivatives by isocratic HPLC." RSC Advances 6, no. 16 (2016): 13120–28. http://dx.doi.org/10.1039/c5ra25854e.

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Amino acids are transformed by nitrosation with dinitrogen trioxide into their corresponding α-hydroxy acids, which are separated and analysed by HPLC, and used to quantify the original amino acid concentration in samples.

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18

Wang, Tao. "Elution Separation of Polygonum Multiflorum Extract under HPLC Isocratic Elution." Applied Mechanics and Materials 455 (November 2013): 33–38. http://dx.doi.org/10.4028/www.scientific.net/amm.455.33.

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In this experiment, the effective compounds of Stilbene glucoside and anthraquinones from polygonum multiflorum extract were separated under HPLC isocratic elution. SinoChrom ODS-BP C18 is selected as chromatographic column. The optimal experimental conditions under 320nm and 254nm wavelength UV detector include the column temperature: 28°C, mobile phase: methanol/water, and flow rate: 1.0ml/mol. All as above, we can draw a conclusion that the technology of stilbene glucoside extraction is successful , the main ingredients of fleece-flower root extract can be effectively separated because the

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19

Kumar, Adarsh M., Mahendra Kumar, Benny J. Fernandez, Karl Goodkin, Neil Schneiderman, and Carl Eisdorfer. "An Isocratic HPLC-ECD Assay of Urinary Normetanephrine and Metanephrine." Journal of Liquid Chromatography 18, no. 11 (1995): 2257–69. http://dx.doi.org/10.1080/10826079508010269.

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20

Djordjevic, Nebojsa M., Fabrice Houdiere, Patrick Fowler, and Francois Natt. "HPLC Separation of Oligonucleotides in Isocratic and Temperature-Programming Mode." Analytical Chemistry 70, no. 9 (1998): 1921–25. http://dx.doi.org/10.1021/ac9711697.

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21

Olgemöller, B., T. Deufel, E. Schleicher, and K. D. Gerbitz. "Rapid determination of serum suramin levels using isocratic HPLC separation." Fresenius' Zeitschrift für analytische Chemie 324, no. 3-4 (1986): 356. http://dx.doi.org/10.1007/bf00487991.

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22

Taşdelen, Betül, Aysun Ergun, and Fikret Büyükkaya. "Rapid isocratic HPLC investigation of radiochemical purity for 90Y-DOTATATE." Journal of Radioanalytical and Nuclear Chemistry 289, no. 2 (2011): 573–75. http://dx.doi.org/10.1007/s10967-011-1119-6.

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23

Naoto Furusawa. "Pipetting sample preparation with water eluent followed by water mobile phase HPLC analysis for residual monitoring of melamine in milk." World Journal of Chemical and Pharmaceutical Sciences 2, no. 1 (2023): 017–22. http://dx.doi.org/10.53346/wjcps.2023.2.1.0032.

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The author introduces a small-scale, time shortening, economical sample preparation with water eluent followed by an isocratic water phase HPLC system for quantifying melamine in cow’s milk. Sample preparation is achieved by homogenization using a handheld ultrasonic-homogenizer with water followed by MonoTipⓇC18 pipette tip contains silica monolith bonded with octadecyl group with water eluent. For determination and identification of analyte, the HPLC uses an analytical C18 column, an isocratic 100% water, and a photo-diode array detector. The procedure, performed under a 100% water condition

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24

Awni, W. M., and L. J. Bakker. "Antipyrine, indocyanine green, and lorazepam determined in plasma by high-pressure liquid chromatography." Clinical Chemistry 35, no. 10 (1989): 2124–26. http://dx.doi.org/10.1093/clinchem/35.10.2124.

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Abstract This HPLC method for measuring antipyrine, lorazepam, and indocyanine green in 0.5 mL of plasma can be used in studies of liver function in which these "model" compounds are used. After a fast, simple, one-step extraction procedure with acetonitrile, an isocratic HPLC system is used, with a single detection wavelength (214 nm) and a single internal standard (1-acetamidopyrene). The mobile phase is a 47/53 (by vol) mixture of acetonitrile and 50 mmol/L phosphate buffer, pH 6. The three compounds are separated on an LC-18 reversed-phase column. The low cost of the HPLC method makes feas

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25

Fatimah, Siti Fatmawati, Endang Lukitaningsih, Ronny Martien, and Akhmad Kharis Nugroho. "Validation of Analytical Method for Vitamin A in Bioadhesive Ocular Cationic Nanoemulsion Loaded into Thermosensitive Gel Using RP-HPLC." Indonesian Journal of Chemistry 24, no. 5 (2024): 1349. http://dx.doi.org/10.22146/ijc.93395.

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Various test methods have been previously documented for determining vitamin A levels in different dosage forms. This study specifically examines an isocratic reverse phase-high performance liquid chromatography (RP-HPLC) method designed for the direct extraction of vitamin A. The objective is to validate an analytical method for quantifying vitamin A in bioadhesive cationic nanoemulsions incorporated into thermosensitive gels. The method employs isocratic RP-HPLC with a YMC-Triart C18 column (L1), dimensions of 4.6 mm × 250 nm, particle size of S-5 µm, and a UV detector at λ = 265 nm. The mob

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26

Shaikh, Siddiqua, and Prerana Jadhav. "RP-HPLC Method Development and Validation for the Simultaneous Estimation of Amitriptyline Hydrochloride and Pantoprazole Sodium in Bulk and Capsule Dosage Form." Journal of Drug Delivery and Therapeutics 9, no. 4 (2019): 37–42. http://dx.doi.org/10.22270/jddt.v9i4.2978.

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A simple, rapid, accurate and precise isocratic reversed phase high performance liquid chromatographic method has been developed and validated for Simultaneous Estimation of Pantoprazole Sodium (PNT) and Amitriptyline Hydrochloride in Bulk and Capsule Dosage Form. In RP-HPLC method, the analyte were resolved by using isocratic program, methanol and phosphate buffer (80:20v/v) was used as mobile phase, at a flow rate of 0.8 ml/min. on HPLC system containing UV-visible detector with Workstation software and cosmosil 18 (250 mm × 4.6 mm,5 μm) column. The detection was carried out at 244nm. The re

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27

Ibrahim, Nasir, Musa Aminu, Abdullahi Musa Ismail, Yusuf Amina Jega, and Awwalu Salisu. "RP-HPLC Method Developed for the Determination of Metformin in Human Saliva." Journal of Pharmaceutical Research 23, no. 1 (2023): 37–41. http://dx.doi.org/10.18579/jopcr/v22.1.ms230206.

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Several extraction steps involved while analyzing metformin in plasma necessitates the need to develop a simple less tedious RP-HPLC method for metformin analysis in human saliva.Blank saliva (2 mL) was spiked with 2 mL solution (12.5 µg mL-1) of metformin and 1 mL solution (0.5 µg mL-1) of caffeine as internal standard (IS). The mixture was vortex mixed and centrifuged at 3000 rpm for 10 minutes. A portion (0.5 mL) of the resultant solution was injected into the HPLC machine (Agilent 1260 infinity). The optimized conditions included a mobile phase of methanol:water (80:20 v/v) containing 0.1

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28

Mayuri, Bhokare* Dr. Pradyumna Ige. "Analytical Method Development and Validation of Dapagliflozin By RP-HPLC Method In Tablet Dosage Form." International Journal of Pharmaceutical Sciences 2, no. 7 (2024): 1718–28. https://doi.org/10.5281/zenodo.12801306.

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The main aim of the present research work is to develop a sensitive, precise and accurate HPLC (High-Performance Liquid Chromatography) procedure for the selective estimation of Dapagliflozin. An isocratic separation of Dapagliflozin through column used was a Inertsil ODS-3V with dimensions of 150 mm length and 4.6 mm inner diameter, packed with 5μm particle utilizing mobile phase composition of Acetonitrile and Water, with a proportion of 50% Acetonitrile and 50% water (v/v).  The detection of the analyte was processed at the maximum wavelength of 223 nm and with 1 ml/min flow of the

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Sayali, B. Wakchaure* Rishikesh Bachhav Smita S. Aher. "RP-HPLC Method Development & Validation For Estimation Of Linezolid In Bulk Drug And Solid Dosage Form." International Journal in Pharmaceutical Sciences 2, no. 7 (2024): 1285–93. https://doi.org/10.5281/zenodo.12761309.

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The main aim of the present research work is to develop a sensitive, precise and accurate HPLC (High-Performance Liquid Chromatography) procedure for the selective estimation of Linezolid. An isocratic separation of Linezolid through column used was a Phenomenex ODS-3 with dimensions of 250 mm length and 4.6 mm inner diameter, packed with 5μm particle utilizing mobile phase composition of Methanol and 0.1% Ortho Phosphoric Acid in water, with a proportion of 70% Methanol and 30% water (v/v).  The detection of the analyte was processed at the maximum wavelength of 258 nm and with 1 ml/m

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30

Galettis, Peter, Michelle Williams, Rebecca Gordon, and Jennifer H. Martin. "A Simple Isocratic HPLC Method for the Quantitation of 17 Cannabinoids." Australian Journal of Chemistry 74, no. 6 (2021): 453. http://dx.doi.org/10.1071/ch20380.

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Although cannabis has been used for several thousand years, the exact composition of the cannabinoids patients are administered for different symptoms has remained largely unknown. While this absence of catalogued information may be accepted in some cultures, the use of cannabis as a human product in the registered medicines setting requires knowing its composition so that doses can be standardised between patients. This is particularly so in clinical trials that are currently under way to determine the efficacy of a product. Although the major cannabinoids of interest to prescribers are well

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31

Trathnigg, Bernd, and Martin Mittelbach. "Analysis of Triglyceride Methanolysis Mixtures Using Isocratic HPLC With Density Detection." Journal of Liquid Chromatography 13, no. 1 (1990): 95–105. http://dx.doi.org/10.1080/01483919008051790.

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32

León-Ruiz, Virginia, Soledad Vera, Amelia V. González-Porto, and María Paz San Andrés. "Analysis of Water-Soluble Vitamins in Honey by Isocratic RP-HPLC." Food Analytical Methods 6, no. 2 (2012): 488–96. http://dx.doi.org/10.1007/s12161-012-9477-4.

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33

Ehlert, Steffen, Lukas Trojer, Martin Vollmer, Tom van de Goor, and Ulrich Tallarek. "Performance of HPLC/MS microchips in isocratic and gradient elution modes." Journal of Mass Spectrometry 45, no. 3 (2010): 313–20. http://dx.doi.org/10.1002/jms.1719.

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34

Kotal, Petr, Milan Jirsa, Pavel Martásek, and Václav Kordač. "Solid phase extraction and isocratic separation of urinary porphyrins by HPLC." Biomedical Chromatography 1, no. 4 (1986): 159–62. http://dx.doi.org/10.1002/bmc.1130010406.

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35

Doneva, Dragica, and Biljana Bauer. "Determination of quercetin in dietary supplements by isocratic RP-HPLC method." Macedonian Pharmaceutical Bulletin 67, no. 2 (2021): 63–72. http://dx.doi.org/10.33320/maced.pharm.bull.2021.67.02.006.

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Quercetin is a natural flavonoid found abundantly in vegetables and fruits. It shows antioxidant, anti-inflammatory, antihepatotoxic, antiallergic, antidiabetic and antiviral activity. Increasingly popular dietary supplements containing quercetin require critical examination of their quality. The aim of this study was to develop a simple and accurate HPLC method for quercetin determination in dietary supplements. Chromatographic separation was achieved by isocratic method, using a Purospher STAR® RP-18 reverse-phase column (150 x 4.6 mm i.d., particle size 5 μm), a mobile phase composed of ace

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Dillip Kumar Mohapatra, Rajesh K Nayak, Deepika Rani Panigrahi, and Surya Narayan Das. "Validation of Finasteride Tablets USP by HPLC Isocratic with UV Detector." International Journal of Scientific Research in Science and Technology 12, no. 1 (2025): 106–13. https://doi.org/10.32628/ijsrst25121163.

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The aims of this work were to validate the analytical method, and evaluate the fenasteride in bulk drug commercial products by HPLC. Fenasteride was eluted from a Kromasil ODS C18, 150 mm x 4.6 mm, 5 at laboratory temperature (30 ± 2º C) with a mobile phase consisting of Acetonitrile and 2.5M Ortho phosphoric acid (50:50 %v/v) at a flow rate of 1.0 mL/min with UV detection at 240 nm. The retention time was about 3.45 minutes and each analysis took not more than 8 minutes. The result obtained for each parameter including Specificity, System Precision, Method Precision (Repeatability), Intermed

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Patel, Harsha U., Bhanubhai N. Suhagia, and Chhaganbhai N. Patel. "Development and Validation of a High-Performance Liquid Chromatographic Method for Determination of Eprosartan in Bulk Drug and Tablets." Journal of AOAC INTERNATIONAL 93, no. 6 (2010): 1862–67. http://dx.doi.org/10.1093/jaoac/93.6.1862.

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Abstract A simple, precise, and accurate isocratic RP-HPLC method was developed and validated for determination of eprosartan in bulk drug and tablets. Isocratic RP-HPLC separation was achieved on a Phenomenex C18 column (250 4.6 mm id, 5 m particle size) using the mobile phase 0.5 formic acidmethanolacetonitrile (80 25 20, v/v/v, pH 2.80) at a flow rate of 1.0 mL/min. The retention time of eprosartan was 7.64 0.05 min. The detection was performed at 232 nm. The method was validated for linearity, precision, accuracy, robustness, solution stability, and specificity. The method was linear in th

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38

Fletcher, Stephen J., and Robby A. Bacchus. "A Simple Improved Method for the Measurement of Cyclosporin by Liquid-Liquid Extraction of Whole Blood and Isocratic HPLC." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 25, no. 5 (1988): 510–15. http://dx.doi.org/10.1177/000456328802500506.

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The current HPLC methods of cyclosporin measurement have been reviewed and all aspects assessed. A simple isocratic C-18 reverse phase HPLC method with improved efficiency is described for the routine measurement of cyclosporin in whole blood. An alkaline ether extraction is followed by an acid wash, solvent evaporation and two hexane washes of the reconstituted extract. The turn-round time for a single sample is 1 h. Daily batches of up to 40 patient samples can be easily measured with this method. The results are compared with those from the Sandoz radioimmunoassay (RIA) method.

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B, Siva Sai Kiran, and Raja S. "Development of validated method for the determination of exemestane by using RP-HPLC." International Journal of Research in Pharmaceutical Sciences 9, no. 1 (2018): 185. http://dx.doi.org/10.26452/ijrps.v9i1.1235.

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A novel RP-HPLC validated method for determination of Exemestane is developed. The chromatographic separation was done on Phenomenex Luna reversed phase C18 (150 mm x 4.6 mm, 5 μm) column in isocratic mode, using Acetonitrile: HPLC grade water (50:50, v/v) as mobile phase at 254 nm wavelength. Exemestane chromatographic peak is eluted with retention time 4.656 min. The linearity range was 5-30 μg/ml with correlation coefficient 0.999. The method was validated as per ICH Guidelines. The quantification and detection limit for estimation of Exemestane was found to be 0.365 and 1.926 μg/ml respect

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40

Gade, Sammaiah*. "Development of Stability Indicating New RP-HPLC Method and Validation for The Estimation of Rucaparib in Pure Form and Marketed Pharmaceutical Dosage Form." International Journal of Pharmacy and Biological Sciences (IJPBS) 13, no. 3 (2023): 48–58. https://doi.org/10.5281/zenodo.10260863.

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AbstractObjective: The current investigation was pointed at developing and progressively validating novel, simple, responsive, and stable RP-HPLC method for the measurement of active pharmaceutical ingredient and Marketed Pharmaceutical Dosage form of Rucaparib. Methods: A simple, selective, validated, and well-defined stability that shows isocratic RP-HPLC methodology for the quantitative determination of Rucaparib. The chromatographic strategy utilized Symmetry ODS (C18) RP Column, 250 mm x 4.6 mm, 5μm, using isocratic elution with a mobile phase of Phosphate Buffer (0.02M) and Acetonitrile

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El-Bagary, Ramzia I., Ehab F. Elkady, Shereen Mowaka, and Maria Attallah. "Validated HPLC and Ultra-HPLC Methods for Determination of Dronedarone and Amiodarone Application for Counterfeit Drug Analysis." Journal of AOAC INTERNATIONAL 98, no. 6 (2015): 1496–502. http://dx.doi.org/10.5740/jaoacint.15-054.

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Abstract Two simple, accurate, and precise chromatographic methods have been developed and validated for the determination of dronedarone (DRO) HCl and amiodarone (AMI) HCl either alone or in binary mixtures due to the possibility of using AMI as a counterfeit of DRO because of its lower price. First, an RP-HPLC method is described for the simultaneous determination of DRO and AMI. Chromatographic separation was achieved on a BDS Hypersil C18 column (150 × 4.6 mm, 5 μm). Isocratic elution based on potassium dihydrogen phosphate buffer with 0.1% triethylamine pH 6–methanol (10 + 90, v/v) at a f

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Furusawa, Naoto. "A 100% Water Mobile Phase HPLC-PDA Analysis of Selected Neonicotinoid Insecticides." JOURNAL OF ADVANCES IN CHEMISTRY 10, no. 9 (2014): 3127–32. http://dx.doi.org/10.24297/jac.v10i9.878.

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This paper describes a reserved-phase HPLC method for detecting frequently-used neonicotinoid insecticides, acetamiprid (ATP) and imidacloprid (ICP), using an isocratic 100 % water mobile phase. Chromatographic separations were performed an Inertsil® WP300 C4 with water mobile phase and a photodiode-array detector. The total run time was < 7 min. The system suitability was well within the international acceptance criteria. The detection limits were 0.013 μg ml-1 for ATP and 0.015 μg ml-1 for ICP, respectively. A harmless HPLC method for simultaneous detecting ATP and ICP was developed and m

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Ribeiro Souza Magalhães, Milena Cristina, Alisson Samuel Portes Caldeira, Hanna De Sousa Rocha Almeida, Sílvia Ligório Fialho, and Armando Da Silva Cunha Junior. "DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD DETERMINATION OF ZIDOVUDINE ENCAPSULATED IN PCL NANOPARTICLES." Drug Analytical Research 1, no. 2 (2017): 1–8. http://dx.doi.org/10.22456/2527-2616.79216.

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A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of encapsulation efficiency of zidovudine in nanoparticules. The method was carried out in isocratic mode using 0.040M sodium acetate: methanol: acetonitrile: glacial acetic acid (880:100:20:2) as mobile phase, a C8 column at 25ºC and UV detection at 240 nm. The method was linear (r2 ˃ 0.99) over the range of 25.0-150.0 μg/mL, precise (RSD ˂ 5%), accurate (recovery = 100.5%), robust and selective. The validated HPLC-UV method can be successfully applied to determine the rate

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Kachhadia, Pankaj K., Ashish S. Doshi, and Hitendra S. Joshi. "Development and Validation of a Stability-Indicating Column High-Performance Liquid Chromatographic Assay Method for Determination of Nebivolol in Tablet Formulation." Journal of AOAC INTERNATIONAL 91, no. 3 (2008): 557–61. http://dx.doi.org/10.1093/jaoac/91.3.557.

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Abstract A simple, precise, and accurate isocratic reversed-phase (RP) stability-indicating column high-performance liquid chromatographic (HPLC) assay method was developed and validated for determination of nebivolol in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on a Phenomenex Luna C8 (2) column (250 mm 4.6 mm id, 5 m particle size) using mobile phase composed of acetonitrilepH 3.5 phosphate buffer (35 + 65, v/v) at a flow rate of 1.0 mL/min, and detection was performed at 280 nm using a photodiode array detector. The drug was subjected to oxidation, hydroly

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Akanksha, D. Punekara* Sonali A. Waghmarea Aaditi S. Punekarb. "Analytical Method Development And Validation For The Estimation Of Azelnidipine In Bulk Drug And Pharmaceutical Dosage Form." International Journal in Pharmaceutical Sciences 2, no. 9 (2024): 803–10. https://doi.org/10.5281/zenodo.13770597.

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The current study covers the invention of an isocratic RP-HPLC method that is straightforward, affordable, quick, accurate, and precise for estimating the amount of bulk azelnidipine and in its commercial products. An evaluation was conducted at 260 nm, which was determined to be Azelnidipine's λmax. The Cosmosil C18 Column, measuring 250 mm by 4.6 mm and 5µm, was used to establish the straightforward, isocratic, and selective RP-HPLC method for azelnidipine. The mobile phase was made up of methanol at pH 6 with 1% formic acid with OPA Water (58: 42% V/V) with a 1.0 ml/min flow ra

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Ameti, Agim, Jasmina Slavkovska, Katerina Starkoska, and Zorica Arsova-Sarafinovska. "A simple isocratic RP-HPLC method for quality control of oseltamivir capsules." Macedonian Journal of Chemistry and Chemical Engineering 31, no. 2 (2012): 205. http://dx.doi.org/10.20450/mjcce.2012.10.

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A simple isocratic reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for determination of oseltamivir active pharmaceutical ingredient (API) in bulk drug and pharmaceuticals. The separation was achieved on a Purospher STAR® RP – 18e column with a mobile phase consisting of methanol- 0.02 mol l-1 phosphate buffer, pH 5, 50:50 (v/v). Chromatographic results demonstrated the specificity of the method for determination of oseltamivir in presence of degradation products generated in studies of forced decomposition. The limit of detection (LOD) and limit of quanti

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Reynolds, T. M., and A. Brain. "A Simple Internally-Standardised Isocratic HPLC Assay for Vitamin B6in Human Serum." Journal of Liquid Chromatography 15, no. 5 (1992): 897–914. http://dx.doi.org/10.1080/10826079208018843.

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Fathy, M. E., S. Abo El Abass Mohamed, H. Elmansi, and F. Belal. "Simultaneous Determination of Cromolyn Sodium Combined Dosage Forms Using Isocratic HPLC Method." Journal of Chromatographic Science 55, no. 1 (2016): 14–22. http://dx.doi.org/10.1093/chromsci/bmw142.

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Supko, Jeffrey G., and Louis Malspeis. "A Rapid Isocratic HPLC Assay of Suramin (NSC 34936) in Human Plasma." Journal of Liquid Chromatography 13, no. 4 (1990): 727–41. http://dx.doi.org/10.1080/01483919008051816.

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Zoest, A. R., J. E. Watson, C. T. Hung, and S. Wanwimolruk. "A Rapid Isocratic HPLC Assay for Dipyridamole Using a Microbore Column Technique." Journal of Liquid Chromatography 14, no. 10 (1991): 1967–75. http://dx.doi.org/10.1080/01483919108049666.

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