To see the other types of publications on this topic, follow the link: Isolant de Bande.
Journal articles on the topic 'Isolant de Bande'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Isolant de Bande.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Murtadha, Murtadha, M. Abduh Ulim, and Syamsuddin Syamsuddin. "Eksplorasi Rizobakteri Indigenous Dan Uji Antagonis Terhadap Patogen Rigidoporus microporus Dan Phellinus noxius Pada Tanaman Karet (Hevea brasiliensis) Secara In Vitro." Jurnal Ilmiah Mahasiswa Pertanian 3, no. 4 (April 4, 2020): 43–52. http://dx.doi.org/10.17969/jimfp.v3i4.9198.
Full textAbstract:
Abstrak. Penelitian bertujuaan untuk mendapatkan isolat rizobakteri yang mampu berperan sebagai agens biokontrol terhadap pengendalian patogen R. microporus dan P. noxius secara in vitro serta sebagai agen rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh, mulai Oktober sampai Desember 2017. Penelitian ini menggunakan Rancangan Acak Lengkap non faktorial. Faktor yang diteliti yaitu isolat rizobakteri, taraf yang dicobakan terdiri dari 15 isolat dan dua patogen antagonis R. microporus dan P. noxius, yang diulang sebanyak 3 kali sehingga terdapat 90 unit satuan percobaan. Hasil penelitian menunujukkan isolat rizobakteri mampu menekan pertumbuhan koloni cendawan patogen. Pada patogen uji R. microporus terdapat 3 rizobakteri yang paling baik yaitu isolat DLG5/3 dengan persentase penghambatan 68,33%, DLG4/1 dengan persentase penghambatan 66,66% dan DLG4/7 dengan persentase penghambatan 63,33%. Pada patogen uji P.noxius terdapat dua rizobakteri yang paling baik dalam menghambat pertumbuhan koloni cendawan patogen yaitu isolat DLG5/1 dengan persentase penghambatan 60,33%, dan DKP6/3 dengan persentase penghambatan 52,50%. Pada laju penghambatan isolat rizobakteri yang paling baik pada patogen R. microporus yaitu isolat DLG6/4 dan DKP4/1 dengan nilai rerata laju penghambatan 20,33 mm/hari. Pada patogen P. noxius menunjukan isolat rizobakteri yang paling baik yaitu isolat DLG4/1 dengan nilai laju penghambatan 12,05 mm/hari.Exploration of Indigenous Rizobacteria and Antagonistic Test against Patogen Rigidoporus microporus And Phellinus noxius In Rubber Plant (Hevea brasiliensis) In VitroAbstract. The research was conducted to obtain rhizobacteria isolates capable of acting as biocontrol agents on pathogen control of R. microporus and P. noxius in vitro and as plant growth promoter rhizobacteria (PGPR). The research was conducted at the Science and Technology of seed Laboratory, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh, starts from October to December 2017. The research using Completely Randomized Design non factorial. Factors researched were rhizobacteria isolates, the experimental stage consisted of 15 isolates and 2 antagonist were R. microporus dan P. noxius, and 3 time repeated until be found 90 units of treatment. The results showed that rhizobacteria isolates capable to inhibith growth of colonies pathogenic. In the pathogen of R. microporus test, there are 3 better rhizobacteria were DLG5/3 isolate with 68.33% inhibition percentage, DLG4/1 with 66.66% inhibition percentage and DLG4/7 with 63,33% inhibition percentage. In the pathogen of P. noxius there are 2 best rhizobacteria inhibiting growth, DLG5/1 isolate with 60.33% inhibition percentage, and DKP6/3 with 52,50% inhibition percentage. In the pathogen of R. microporus test, there are 2 better rhizobacteria were DLG6/4 and DKP4/1 with 20,33 mm/day inhibition percentage. In the pathogen of P. noxius test, the best rhizobacteria were DLG4/1 with 12,05 mm/day inhibition percentage.
2
Tilley, Michael, and Steve J. Upton. "Electrophoretic characterization of Cryptosporidium parvum (KSU-1 isolate) (Apicomplexa: Cryptosporidiidae)." Canadian Journal of Zoology 68, no. 7 (July 1, 1990): 1513–19. http://dx.doi.org/10.1139/z90-224.
Full textAbstract:
Sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblotting, lectin binding, and iodine-125 surface labeling of sporozoites were used to probe the coccidial pathogen, Cryptosporidium parvum. Analysis of silver-stained profiles of CsCl gradient purified, freeze–thawed oocysts revealed >50 bands, whereas profiles of anion exchange chromatography purified sporozoites displayed >40 bands. Surface iodination of sporozoites revealed about 20 surface proteins, the most heavily labeled of which had molecular masses of 18–20, 37–39, 48, 73–76, and 102–105 kilodaltons. Following electrophoresis and Western blotting, 4 of 12 different 125I-labeled lectin probes bound to blots and collectively revealed at least 19 bands to be glycosylated. Concanavalin A specifically recognized at least 14 bands, whereas Griffonia simplicifolia agglutinin I, Maclura pomifera agglutinin, and soybean agglutinin recognized 5–7 bands each. Of the five most heavily labeled surface molecules, the four with lowest molecular masses all appeared to be glycosylated. Immunoblotting with sera collected from two individuals 30 days after they passed oocysts revealed that the majority of parasite glycoproteins and surface molecules are immunogenic.
3
Praja, Ratih Novita, Didik Handijatno, Setiawan Koesdarto, and Aditya Yudhana. "Karakterisasi Protein VirB4 Brucella abortus Isolat Lokal dengan Teknik Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis." Jurnal Veteriner 18, no. 3 (September 4, 2017): 416. http://dx.doi.org/10.19087/jveteriner.2017.18.3.416.
Full textAbstract:
Brucellosis is a zoonotic disease cause by pathogenic bacteria of the genus Brucella. The disease infects livestock mammals such as cows, goats, pigs, and including humans. Brucella abortus has several potential virulence factors, i.e. Proteins VirB. Type IV secretion system (T4SS) which is a combination of 12 proteins from VirB1-VirB11 and VirD4. Brucella can survive for long periods in the environment despite the limitations of nutrients and oxygen. This study aims to characterize the protein VirB4 of local isolate of B. abortus using SDS PAGE (Sodium Dodecly sulfate-polyacrylamide gel electrophoresis). The results showed that the protein contained 10 protein bands with a molecular weight of 158.93; 110.89; 99.931; 70.60; 64.61; 59.25; 45.32; 42.35; 23.63; and 16.70 kDa, respectively. Protein VirB4 of the local isolate of B. abortus have a molecular weight of 59.25 kDa.
ABSTRAK
Brucellosis merupakan salah satu penyakit zoonosis yang disebabkan oleh bakteri patogen genus Brucella. Penyakit ini menyerang hewan ternak mamalia seperti sapi, kambing, babi, dan dapat menular ke manusia. Bakteri Brucella abortus memiliki faktor virulensi potensial yaitu protein VirB. Type IV Secretion System (T4SS) merupakan gabungan dari 12 protein yaitu VirB1–VirB11 dan VirD4. Brucella dapat bertahan hidup lama di dalam lingkungan meskipun memiliki keterbatasan nutrisi dan oksigen. Penelitian ini dilakukan untuk karakterisasi protein VirB4 B. abortus isolat lokal dengan metode Sodium Dodecly Sulfate-Polyacrylamide Gel Electrophoresis (SDS PAGE). Hasil karakterisasi protein B. abortus isolat lokal dengan teknik SDS-PAGE terdapat 10 pita protein dengan bobot molekul 158,93; 110,89; 99,931; 70,60; 64,61; 59,25; 45,32; 42,35; 23,63; dan 16,70 kDa. Simpulan penelitian ini adalah terdapat protein VirB4 B. abortus isolat lokal yang mempunyai bobot molekul 59,25 kDa.
4
Collisson, Ellen W., T. Lynwood Barber, Colleen M. Shannon, and Maurice C. Kemp. "Genotypic Transitions among Bluetongue Viral Isolates from Domestic Ruminants in Colorado during 1981–1984." Journal of Veterinary Diagnostic Investigation 1, no. 3 (July 1989): 242–46. http://dx.doi.org/10.1177/104063878900100309.
Full textAbstract:
Two predominant electropherotypes of bluetongue virus (BTV) serotype 11 isolates from cattle during a 1981–1984 field study in eastern Colorado were characterized. The genomes of strains isolated from the first 2 years of the study had 1 predominant electropherotype (CO81), with the exception of 1 isolate that differed only in the migration of segment 3. A second electropherotype (CO83), with differences in the migration of 4 segments, coexisted in the same region during 1983 and 1984 with strains having the CO81 RNA profile. The genomes of CO81 and CO83 were also distinguishable from those of the US prototype of BTV 11. Analysis of the polypeptides of representative strains of each electropherotype by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the proteins were very similar. The occurrence of the CO81 electropherotype was apparently the result of multiple viral infections since the positions of 7 segments had faint second bands and single-banded variants were isolated after serial plaque purifications. In addition, protein 7 of 1 of the CO81 isolates and protein 7 of the single-banded variant differed as shown by reverse phase-high performance liquid chromatography of 35S-methionine-labeled tryptic peptides.
5
Jeng, Robert S. "Analytical electrofocusing and two-dimensional electrophoresis of proteins extracted from the mycelia of aggressive and nonaggressive strains of Ophiostoma ulmi." Canadian Journal of Botany 64, no. 9 (September 1, 1986): 2073–81. http://dx.doi.org/10.1139/b86-272.
Full textAbstract:
Soluble mycelial proteins from Ophiostoma ulmi (Buism.) Nannf., the causal agent of Dutch elm disease, were separated by analytical electrofocusing and two-dimensional electrophoresis in polyacrylamide gels. Results showed the aggressive and nonaggressive strains of this pathogen each had about 60 Coomassie blue stained bands having isoelectric points from 3 to 7. Both strains of this fungus had their own characteristic electrofocusing patterns. Nonaggressive isolate S116, for example, lacked two protein bands, one near the anode and one near the cathode, but it had five additional protein bands distributed from pH 4 to 6. Two-dimensional electrophoresis of total soluble proteins depicted that there were 36 proteins found to be specific for the nonaggressive isolate S116 and 12 proteins for the aggressive isolate RR2.
6
Kamel, Ehab Abdel-Razik, and M. Elsayed Rashed. "Electrophoretic Protein Banding Patterns among Penicillium Strains Isolated from Saudi Arabia." International Journal of Applied Sciences and Biotechnology 2, no. 3 (September 25, 2014): 283–90. http://dx.doi.org/10.3126/ijasbt.v2i3.10949.
Full textAbstract:
14 strains of Penicillium species were isolated from different localities and habitats from Jeddah, Saudi Arabia and cultivated on two different media: Czapek Dox’s medium, in which NaNO3 is the source of inorganic nitrogen, and Waksman’s medium, in which pepton is the source of organic nitrogen. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) technique was used in this study to distinguish these isolates. The Penicillium isolates examined in this study consisted of six Penicillium species: Penicillium corylophilum (three isolates), P. rubrum (one isolate), P. citrinum (two isolates), P. crustosum (one isolate), P. canesens (six isolates) and Penicillium sp. (one isolate). The electrophoretic protein patterns from Penicillium isolates grown on Czapek Dox's medium revealed the presence of 17 different bands (out of 14 polymorphic bands, there were three monomorphic bands and two unique bands). The electrophoretic protein pattern of the same isolates grown on Waksman's medium revealed the presence of 12 different bands (out of eight polymorphic bands, there were four monomorphic bands and one unique band). Data were analysed by a clustering method and similarity coefficients using NTSYSpc version 2.02i. Two different phenograms were produced for the studied Penicillium species based on the analysis of the protein banding patterns. Data from the protein banding patterns produced from both media were combined and analysed to produce third phenogram, and the relationships between the species and isolates are discussed. DOI: http://dx.doi.org/10.3126/ijasbt.v2i3.10949Int J Appl Sci Biotechnol, Vol. 2(3): 283-290
7
Abe, Niichiro, Kazutoshi Takami, Isao Kimata, and Motohiro Iseki. "Molecular Characterization of a Cryptosporidium Isolate From a Banded Mongoose Mungos mungo." Journal of Parasitology 90, no. 1 (February 2004): 167–71. http://dx.doi.org/10.1645/ge-3231rn.
Full text
8
Wszolek, B., and W. Godlowski. "Toward an adequate method to isolate spectroscopic families of diffuse interstellar bands." Monthly Notices of the Royal Astronomical Society 338, no. 4 (February 1, 2003): 990–98. http://dx.doi.org/10.1046/j.1365-8711.2003.06143.x.
Full text
9
Poplawski, Agnieszka M., John A. G. Irwin, and John M. Manners. "Isolation of Genotype- and Chromosome-specific DNA Markers in a Biotype of Colletotrichum gloeosporioides Using Random Amplified Polymorphic DNA Analysis." Australian Journal of Botany 46, no. 1 (1998): 143. http://dx.doi.org/10.1071/bt96131.
Full textAbstract:
Genetic markers that distinguish fungal genotypes are important tools for genetic analysis of heterokaryosis and parasexual recombination in fungi. Random amplified polymorphic DNA (RAPD) markers that distinguish two races of biotype B of Colletotrichum gloeosporioides infecting the legume Stylosanthes guianensis were sought. Eighty-five arbitrary oligonucleotide primers were used to generate 895 RAPD bands but only two bands were found to be specifically amplified from DNA of the race 3 isolate. These two RAPD bands were used as DNA probes and hybridised only to DNA of the race 3 isolate. Both RAPD bands hybridised to a dispensable 1.2 Mb chromosome of the race 3 isolate. No other genotype-specific chromosomes or DNA sequences were identified in either the race 2 or race 3 isolates. The RAPD markers hybridised to a 2 Mb chromosome in all races of the genetically distinct biotype A pathogen which infects other species of Stylosanthes as well as S. guianensis. The experiments indicate that RAPD analysis is a potentially useful tool for obtaining genotype- and chromosome-specific DNA probes in closely related isolates of one biotype of this fungal pathogen.
10
Parvin, N., M. Bilkiss, J. Nahar, MK Siddiqua, and MB Meah. "RAPD analysis of Sclerotium rolfsii isolates causing collar rot of eggplant and tomato." International Journal of Agricultural Research, Innovation and Technology 6, no. 1 (August 12, 2016): 47–57. http://dx.doi.org/10.3329/ijarit.v6i1.29212.
Full textAbstract:
Eight isolates of Sclerotium rolfsii from four strategically geographical sites of Bangladesh were characterized and their cultural properties like average linear mycelial growth, colony colour, colony consistency, growth pattern and sclerotia formation were studied. Isolates varied in mycelial growth and other growth characteristics and were grouped into three. The highest linear growth was displayed by S8. DNA concentration of eight isolates varied from 1150-7200 ng/?l. DNA fingerprinting by RAPD prompted the grouping of isolates. Selected 3 primers generated 20 bands with size ranging from 100-1500 bp. Out of the 20 bands, 9 bands (45%) were polymorphic and 11 bands (55%) were monomorphic among the eight isolates of Sclerotium rolfsii. The co-efficient of gene differentiation (Gst) was 1.000 reflecting the existence of high level of genetic variations among the 8 isolates. The lowest genetic distance and highest inter isolate similarity was found in S1 and S2 which would be homogeneous. The highest genetic distance and lowest inter isolate similarity found in S3, S7 and S3, S8 pair which would be most divergent isolates. The cluster analysis also revealed that S3, S7 and S8 belong to different clusters. All five varieties of eggplant and tomatoes were graded as susceptible when inoculated with eight isolates. Plant mortality 93.33% was recorded in S4, S6 and in S8. Considering the isolate factor the most virulent isolate would be S8 whereas the less virulent isolate would be S2 and S7. Host plant of S8 was tomato collected from Thakurgaon. S2 and S7 were collected from BAU farm and Dinajpur and host plants were lentil and tomato respectively. It is evident that Sclerotium rolfsii from Thakurgaon on host tomato is more virulent.Int. J. Agril. Res. Innov. & Tech. 6 (1): 47-57, June, 2016
11
Huslina, Feizia, and Diannita Harahap. "ISOLASI BAKTERI PENGIKAT NITROGEN DENGAN MENGGUNAKAN MEDIA JENSEN." Jurnal Agrotek Ummat 6, no. 2 (August 31, 2019): 91. http://dx.doi.org/10.31764/agrotek.v6i2.1238.
Full textAbstract:
Bakteri pengikat nitrogen adalah jenis bakteri yang paling banyak ditemukan di tanah dan sebagiannya bersimbiosis dengan akar tumbuhan. Bakteri ini mengikat nitrogen di udara dan mengubahnya menjadi nitrat yang kemudian digunakan oleh tumbuhan. Media Jensen adalah media selektif yang umumnya digunakan untuk menumbuhkan bakteri pengikat nitrogen. Penelitian ini bertujuan untuk mengisolasi bakteri pengikat nitrogen dengan menggunakan media Jensen. Bakteri diisolasi dari rizosfer di sekitar UIN Ar-Raniry, Banda Aceh dan dilakukan karakteristik morfologi dan Optical Density (OD). Empat isolat (IS-A, IS-B, IS-C, IS-D) ditemukan pada serial pengenceran 10-2 dan 10-4 dan isolat D (IS-D) memiliki nilai Optical Density (OD) tertinggi yaitu sebesar 0,373.
12
Achmad, E. N. Herliyana, I. Z. Siregar, and O. Permana. "Karakter Morfologis dan Genetik Jamur Tiram (Pleurotus spp.)." Jurnal Hortikultura 21, no. 3 (October 13, 2016): 225. http://dx.doi.org/10.21082/jhort.v21n3.2011.p225-231.
Full textAbstract:
Hutan tropis Indonesia merupakan salah satu pusat keanekaragaman hayati di dunia, yang salah satu di antaranya ialah jamur tiram (Pleurotus spp.). Penelitian ini bertujuan mempelajari karakteristik morfologi dan genetik delapan isolat Pleurotus spp.. Penelitian dilakukan pada bulan September 2005 sampai April 2006 di Laboratorium Patologi Hutan dan Lab. Silvikultur, serta Lab. Bioteknologi Kehutanan dan Mikrobiologi Molekuler, Pusat Studi Hayati, Institut Pertanian Bogor. Tubuh buah delapan isolat jamur digunakan sebagai bahan pengamatan. Hasil penelitian menunjukkan bahwa tubuh buah delapan isolat jamur memiliki warna putih, coklat, atau merah jambu, dengan atau tanpa tangkai, bentuk tudung berupa lingkaran penuh atau setengah lingkaran. Amplifikasi dengan primer RAPD OPO11 menghasilkan 12 pita, satu pita bersifat monomorfik dan 11 pita lainnya bersifat polimorfik yang menunjukkan keragaman pada delapan isolat jamur tiram yang dipelajari. Pengelompokan berdasarkan pola pita amplifikasi primer RAPD tersebut menghasilkan tiga kelompok isolat. Kelompok I terdiri atas isolat Pleurotus sp.17, Pleurotus sp.16, Pleurotus sp.21, Pleurotus sp.27, dan Pleurotus sp.9, kelompok II terdiri atas isolat Pleurotus sp.4 dan Pleurotus sp.5, serta kelompok III yang hanya berisi satu isolat yaitu Pleurotus sp.24. Pengelompokan berdasarkan marka RAPD tersebut sejalan dengan karakteristik morfologinya. Informasi mengenai karakter morfologis dan genetik jamur tiram diharapkan akan bermanfaat untuk pengembangannya sebagai komoditas jamur komersial.<br /><br />Indonesian rainforest is one of the world’s centers of biodiversity, which one of them is the oyster mushroom (Pleurotus spp.). This research was aimed to determine the morphological and genetic characteristics of eight isolates of Pleurotus spp.. The research was conducted from September 2005 to April 2006 at Forestry Pathology Laboratirum and Silvyculture Lab., and Forestry Biotechnology Lab. and Molecular Microbiology, Center of Biology Study, Agricultural Bogor Institute. The mushroom fruit body of the eight isolates was used as the material for observation of morphological and genetic characters. The results showed that the fruit body of eight isolates exhibited white, brown, or pink in color, with or without stalk, and full or half circle of cap shape. Amplification with RAPD primers OPO11 produced 12 bands, which one band was monomorphic while the others were polymorphic that showed the variability of the eight oyster mushroom isolates. Clustering based on banding patterns of amplification primers resulted in three groups. Group I consisted of Pleurotus sp.17, Pleurotus sp.16, Pleurotus sp.21, Pleurotus sp.27, and Pleurotus sp.9 isolates. Group II included Pleurotus sp.4 and Pleurotus sp.5 isolates, while the third group contained only one isolate i.e. Pleurotus sp.24. The molecular grouping was in line with the morphological characters. Information of morphological and genetic characteristics will hopefully give benefit for the development of the oyster mushroom as one of the commercial commodities.<br /><br />
13
Baehaki, Ace, Arif Hidayat, Nuni Gofar, and Rodiana Nopianti. "Production and Characterization of Crude Protease from RS1 Isolate from silage of Floating Bladderwort (Utricularia gibba)." Mediterranean Journal of Chemistry 10, no. 3 (March 25, 2020): 289–93. http://dx.doi.org/10.13171/mjc02003251256ab.
Full textAbstract:
The purpose of this research was to produce and characterizing crude protease from RS1 isolate of swamp plant silage. The optimum production time of RS1 isolate was 40 h. The optimum pH and temperature of protease from RS1 isolate were 10 and 45℃, respectively. Ion Mg3+ increased RS1 protease whereas ion of Na+, K+, Fe2+, and Zn2+ inhibited protease from RS1 isolate. Study on the effect of metals ion indicated that protease from RS1 isolate was metaloenzyme. Based analysis on SDS-PAGE, the molecular weight of RS1 protease had 12 bands with molecular weights ranging from 34.75 kDa to 263.53 kDa.
14
Lestari, Diana. "AKTIVITAS ANTIBAKTERI PEPTIDA KASEIN SUSU KAMBING HIDROLISIS OLEH PAPAIN TERHADAP Pseudomonas aeruginosa ANTIBACTERIAL ACTIVITY OF GOAT MILK CASEIN PEPTIDES HYDROLYZED BY PAPAIN ENZYME TOWARD Pseudomonas aeruginosa." Jurnal Ilmu Pangan dan Hasil Pertanian 1, no. 2 (January 28, 2018): 81. http://dx.doi.org/10.26877/jiphp.v1i2.1914.
Full textAbstract:
AbstrakSusu kambing mengandung sekitar 3.4% protein, termasuk di dalamnya peptida bioaktif. Peptida bioaktif (contohnya peptida antibakteri) adalah fragmen protein spesifik yang bermanfaat bagi fungsi tubuh manusia dan bisa diperoleh melalui proses hidrolisis enzimatik. Penelitian mengenai peptida bioaktif dari susu kambing Indonesia masih belum banyak dilaporkan. Oleh karena itu tujuan dari penelitian ini adalah untuk menganalisis profil protein dan peptida dari kasein susu kambing Etawa yang dihidrolisis oleh papain dan menganalisis aktivitas antibakteri peptida terhadap bakteri Pseudomonas aeruginosa. Kasein diisolasi dari susu kambing segar dan dihidrolisis selama 0, 1, 3, 10, dan 15 menit pada 500C dengan papain. Profil protein dan peptida dianalisis dengan metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) (konsentrasi gel 20%). Hasil analisis menunjukkan bahwa pita protein hasil hidrolisis menjadi semakin tipis seiring dengan bertambahnya waktu hidrolisis. Aktivitas antibakteri dari isolat kasein dan peptida hasil hidrolisis dianalisis menggunakan microplate reader selama 24 jam. Hasil analisis menunjukkan Isolat kasein dan peptida 3 menit menunjukkan aktivitas antibakteri dengan memperpanjang fase lag yang paling baik terhadap terhadap P. aeruginosa dibandingkan peptida lainnya. Kata kunci : antibakteri, kasein, peptida antibakteri, SDS-PAGE, susu kambing AbstractGoat milk contains approximately 3.4% protein, including bioactive peptides. Bioactive peptides (as example antibacterial peptides) are specific protein fragments that have beneficial effect for human body functions and can be obtained by enzymatic hydrolysis process. Research about bioactive peptides from Indonesian goat milk is still a few that has been reported. Therefore the objectives of this research were to analyze protein and peptides profile from Etawa goat milk casein hydrolyzed by papain, and to analyze antibacterial activity of the peptides toward Pseudomonas aeruginosa. Casein was isolated from fresh goat milk and hydrolyzed for 0, 1, 3, 10, and 15 minutes at 500C by papain. Protein and peptides profile were analyzed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method (20% gel). The results showed that protein bands of hydrolyzed casein were getting thinner as the hydrolysis process time increased. Antibacterial activity of casein isolate and hydrolyzed peptides were analyzed by using microplate reader for 24 hours. The results showed that casein isolate and peptides could inhibit the bacterial growth by extending the lag phase of bacterial growth. The casein isolate and peptides hydrolyzed for 3 minutes has the best antibacterial activity toward Pseudomonas aeruginosa. Keywords: antibacterial, antibacterial peptides, casein, goat milk, SDS-PAGE
15
Syahputra, Ade, Kikin Hamzah Mutaqin, and Tri Asmira Damayanti. "Komparasi Metode Isolasi DNA Patogen Antraknosa dan Bulai untuk Deteksi PCR." Jurnal Fitopatologi Indonesia 12, no. 4 (November 21, 2016): 124. http://dx.doi.org/10.14692/jfi.12.4.124.
Full textAbstract:
Polymerase chain reaction (PCR) is an important tool for detection, identification and monitoring of quarantine pests in Indonesia. DNA isolation method from target organism is an important step to provide adequate DNA template for performing PCR. Objective of the research was to compare conventional, commercial kit, FTA-card and its modification methods of DNA isolation to be used in PCR detection for Colletotrichum acutatum and Peronosclerospora sorghi from chili and maize, respectively. DNA obtained from various isolation methods were measured using UV-vis nanodrop-spectrophotometry. DNA amplification was performed using DNA concentration of 15 ng µL-1 from each isolation method with gradual primer concentrations of 0.4; 0.6; 0.8; and 1.0 mM. The highest concentration of DNA was achieved with conventional methods for C. acutatum from pure culture and P. sorghi from maize leaf. Best DNA purity was obtained from isolation method using commercial kit for C. acutatum from infected fruit (1.94) and from conventional method for C. acutatum from pure culture (1.91). The highest total yield of isolated DNA was achieved by modified FTA-card for C. acutatum from pure culture. In general DNA amplification using various primer concentration gave positive results although DNA bands intensity was varied from faint to very bright. Furthermore PCR optimization using the best primer concentration from previous reaction showed that all DNA templates resulted in thick and bright DNA bands.
16
Melizah, Ardesy, Syarif Husin, and Syifa Alkaf. "Identification of Lactic Acid Bacteria Isolate From Fermentation Food Bekasam." Bioscientia Medicina : Journal of Biomedicine and Translational Research 2, no. 1 (January 13, 2018): 16–23. http://dx.doi.org/10.32539/bsm.v2i1.34.
Full textAbstract:
Bekasam is a local food from South Sumatera Indonesia, which is obtained through fermentation of fish. Previous study has shown that fermented food contained Lactic Acid Bacteria (LAB) bacteria, such as Lactobacillus plantarum, Lactobacillus sp, Pediococus sp and Weissella sp. The study was conducted to identify isolate LAB from bekasam. Bekasam contained fish, rice, and salt with a certain ratio. Further isolation of isolated LAB isolate, then performed PCR for bacterial analysis. Isolate 1 showed alleged bacteria Staphylococcus sp, non pathogen, while isolate 2 leads to Lactobacillus sp. The PCR results show the suitability of the bands formed between the Lactobacillus standard and the isolates.
Keywords: bacteria, lactobacillus, acid
17
Zulhaerati, Zulhaerati, Sri Budiarti, and Rika Indri Astuti. "Metagenomic Analysis of Bacteria Phylum Firmicutes and Bacteroidetes in Women with Type 2 Diabetes." HAYATI Journal of Biosciences 26, no. 3 (December 2, 2019): 110. http://dx.doi.org/10.4308/hjb.26.3.110.
Full textAbstract:
Diabetes mellitus (DM) is a serious health problem in Indonesia. Type 2 diabetes is a metabolic disease which primarily caused by obesity-linked insulin resistance. There is a link between insulin resistance and bacterial populations in the gut. Therefore, the aims of this study was to analyze composition of bacteria belong to the phylum Firmicutes and Bacteroidetes in women with type 2 diabetes and non-diabetic persons as control. The study included five female adults type 2 diabetes patients and five healthy controls. The bacteria composition was analyzed for abundance by denaturing gradient gel electrophoresis (DGGE). Metagenomic analysis based on 16S rRNA gene was represented by 12 DGGE bands. The twelve respective bands showed the similarity ranging from 77 up to 98%. Diversity of microbial composition in women type 2 diabetes was represented by five DGGE bands are Bacteroides fragilis, Bacteroides vulgatus, uncultured Bacteroides sp. clone Lb4eF4, uncultured bacterium clone 16sms90-5g05, and uncultured bacterium clone Malaga 1F14. Four DGGE bands in women healthy controls are closely related to uncultured bacterium isolate DGGE gel band Eub42, uncultured bacterium isolate DGGE gel band K115, uncultured bacterium clone HFV04255, and Enterococcus sp. the finding shows bacterial species belong to the phylum Bacteroidetes was mostly found in women diabetic group, while those belong to the phylum Firmicutes was mostly found in healthy controls.
18
Lewis, Megan O., Ylva M. Pihlström, Loránt O. Sjouwerman, and Michael C. Stroh. "Stellar populations in the BAaDE survey." Proceedings of the International Astronomical Union 14, S353 (June 2019): 43–44. http://dx.doi.org/10.1017/s1743921319008111.
Full textAbstract:
AbstractThe BAaDE (Bulge Asymmetries and Dynamical Evolution) project is an SiO maser survey of the Galactic Plane. About 19,000 sources have been observed at 43 GHz with the VLA, and the production of spectra for each of these sources is well underway. The primary goal of the project is to collect line-of-sight velocities for all the detected masers in the sample to probe Galactic dynamics. With an expected detection rate of over 60% we should collect over 11,000 velocities to probe the Galactic potential. The survey is also a large sample of infrared sources to explore the different evolved stellar populations within the Milky Way. So far we discern three distinct groups in the BAaDE sample: the main group containing oxygen-rich, evolved stars with a high SiO maser detection rate, a much smaller population of carbon-rich evolved stars, and finally a group of likely young stellar objects with no maser emission. These populations are separated using 2MASS and MSX color-color diagrams, and we find a particularly useful cut between the young and evolved objects using the MSX [D] –[E] color. Identification of these populations will isolate BAaDE’s evolved star sample, and will more tightly define the region in IR color-color diagrams where SiO masers occur yielding a better understanding of these kinematical probes. Using our color-divisions we can also study the distribution of each of the populations within the Galactic Plane.
19
Retchless, David Pahl. "Communicating climate change: spatial analog versus color-banded isoline maps with and without accompanying text." Cartography and Geographic Information Science 41, no. 1 (September 5, 2013): 55–74. http://dx.doi.org/10.1080/15230406.2013.826479.
Full text
20
Azevedo, Andréia Cristiane Souza, Daniel Ricardo Sosa-Gómez, Marcos Rodrigues Faria, and Maria Helena Pelegrinelli Fungaro. "Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae)." Genetics and Molecular Biology 23, no. 1 (March 2000): 61–63. http://dx.doi.org/10.1590/s1415-47572000000100010.
Full textAbstract:
Bands of double-stranded RNA (dsRNA) were detected in three out of twelve isolates of Paecilomyces fumosoroseus. Identity of these bands was confirmed by RNAse, DNAse and S1 nuclease treatments. The cure of dsRNA for one isolate (P92) was successfully carried out for a single conidium subculture. Isogenic strains, with or without dsRNA, were submitted to virulence tests against the whitefly Bemisia tabaci strain B. In contrast to findings for some phytopathogenic fungi, these dsRNA fragments did not cause hypovirulence in P. fumosoroseus.
21
Lomer, C. J., C. Prior, and C. Kooyman. "DEVELOPMENT OF METARHIZIUM SPP. FOR THE CONTROL OF GRASSHOPPERS AND LOCUSTS." Memoirs of the Entomological Society of Canada 129, S171 (1997): 265–86. http://dx.doi.org/10.4039/entm129171265-1.
Full textAbstract:
AbstractFour research programmes are investigating the entomopathogenic fungal genera Metarhizium and Beauveria for locust and grasshopper control in Africa. In the LUBILOSA programme, surveys for pathogen isolates revealed a morphologically distinctive Metarhizium flavoviride Gams and Rozsypal attacking acridoids in West Africa, Madagascar, and elsewhere. Metarhizium anisopliae (Metschnikoff) Sorokin isolates with virulence to acridoids were also obtained, including several from non-orthopteran hosts. Natural epizootics of both genera are rare in acridoid populations, but do occur. A standardized screening method discriminated virulent from non-virulent isolates. The great majority of the most virulent isolates were from the acridoid group of M. flavoviride. A Niger isolate chosen for development from this group had low virulence to honey bees and parasitic Hymenoptera and was not infective to insects in several other orders. Field tests were carried out on formulations of oil mixtures, using ULV application rates of 1–2 L/ha and 2–5 × 1012 conidia per hectare. In preliminary tests, target insects were sprayed successfully in small field arenas and in large cages. Trials in 1993 on variegated grasshopper gave an approx. 90% reduction in field populations after 15 days. Trials on various acridids, predominantly Hieroglyphus daganensis Krauss, in dense grass in northern Benin showed slower mortality, although up to 70% population reduction was achieved. Trials using a vehicle-mounted ULV sprayer (the Ulva-Mast) in open grassland in Niger gave >90% mortality in samples of mixed acridids. In Mali, a Malian isolate of M. flavoviride was shown to be slightly more virulent than the standard Niger isolate; both isolates gave significant population reductions against nymphs of Oedaleus senegalensis Krauss and Kraussella amabile (Krauss) in 1-ha plots. Successful small-scale field trials have also been carried out using the standard M. flavoviride isolate in South Africa against brown locust and in Australia using an Australian isolate against wingless grasshopper. In Mauritania, a trial using the Niger isolate against desert locust nymph bands gave up to 90% mortality in caged samples by day 9 after spraying. The uncaged treated bands were completely destroyed by predators while untreated bands fledged. Oil-based ULV formulations of M. flavoviride are capable of causing high mortality in the field populations of all acridoids against which they have been field tested and show great promise for development as components of IPM strategies for these pests.
22
Ahyamaqvirah, Rivayani, Nanda Mayani, Nanda Mayani, Syamsuddin Syamsuddin, and Syamsuddin Syamsuddin. "Efektivitas Rizobakteri Isolat Tanaman Tomat (Lycopersicum esculentum Mill.) terhadap Daya Hambat Pertumbuhan Koloni Patogen Fusarium oxysporum Tanaman Terung (Solanum melongena L.) secara in vitro." Jurnal Ilmiah Mahasiswa Pertanian 3, no. 4 (November 1, 2018): 149–58. http://dx.doi.org/10.17969/jimfp.v3i4.9549.
Full textAbstract:
Abstrak. Penelitian ini bertujuan untuk mendapatkan rizobakteri isolat tanaman tomat yang mampu berperan sebagai agen biokontrol dalam menghambat pertumbuhan koloni patogen Fusarium oxysporum tanaman terung secara in vitro. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh dari bulan Mei sampai Juli 2018. Rancangan yang digunakan pada uji antagonisme rizobakteri dengan patogen adalah Rancangan Acak Lengkap (RAL) non faktorial. Parameter yang diamati adalah persentase penghambatan pertumbuhan koloni patogen dan laju penghambatan pertumbuhan koloni patogen. Hasil penelitian menunjukkan persentase daya hambat rizobakteri yang tertinggi dalam menghambat pertumbuhan koloni patogen yaitu isolat SRK 5/3 dengan nilai 61,97% dengan aktivitas tinggi dan laju penghambatan isolat rizobakteri yang menekan pertumbuhan koloni, hifa, dan spora yaitu isolat SRK 5/3 dengan nilai 11,50 mm/hari.EFFECTIVENESS OF RIZOBACTERIAL TOMATO PLANT (Lycopersicum esculentum Mill.) ISOLATES ON PATHOGENIC COLUMN GROWTH POWER OF Fusarium oxysporum EGGPLANT (Solanum melongena L.) IN VITROAbstract. This study aims to obtain rhizobacteria from tomato isolates which are able to act as biocontrol agents for inhibiting the growth of pathogenic colonies of Fusarium oxysporum in eggplant plants by in vitro method. This research was carried out at the Laboratory of Seed Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh, from May to July 2018. The test of rhizobacterial antagonism with pathogens used non-factorial Completely Randomized Design (CRD). The parameters of this research were the percentage of inhibition of the pathogenic colonies growth and the rate of inhibition of the pathogenic colonies growth. The results showed that the highest percentage of rhizobacterial inhibition for hampering the growth of pathogenic colonies, SRK 5/3 isolates with 61.97% values with high activity and the rate of inhibition of rhizobacterial isolates which suppressed the growth of colony, hyphae, and spores, is 5/3 SRK isolates with the value of 11.50 mm / day.
23
USHAKOV, I. V., A. N. VODIN, and G. K. KHOMYAKOV. "POPULATION OF ROTATION BANDS IN γ-DECAY OF ANALOG STATES IN 23Na." International Journal of Modern Physics E 18, no. 04 (April 2009): 1084–87. http://dx.doi.org/10.1142/s0218301309013294.
Full textAbstract:
The γ-decay from [Formula: see text] isobaric analog levels was studied in odd-mass nucleus 23 Na . The investigations were performed using the proton beam at E p = 1623, 1721, 1803 and 1835 keV. It was shown that during γ-decay, mainly low-lying levels of 23 Na are populated; among these levels, we could isolate rotational bands with [Formula: see text] and [Formula: see text], based on the seventh [Formula: see text] and ninth [Formula: see text] orbits of the Nilsson scheme, respectively. The intensities of the M1 transitions were compared with the results of the calculations within the Nilsson model.
24
Hollister, W. S., E. U. Canning, N. I. Colbourn, A. Curry, and C. J. N. Lacey. "Characterization ofEncephalitozoon hellem(Microspora) isolated from the nasal mucosa of a patient with AIDS." Parasitology 107, no. 4 (November 1993): 351–58. http://dx.doi.org/10.1017/s003118200006769x.
Full textAbstract:
SUMMARYA microsporidium of the genusEncephalitozoonwas isolated into culture from the nasal epithelium of a patient with AIDS. It was compared within vitroisolates ofEncephalitozoon cuniculiand the type isolate ofEncephalitozoon hellemby SDS–PAGE and by Western blotting with murine antisera raised toE. cuniculi, E. hellemand the nasal isolate, monoclonal antibodies raised toE. cuniculiand sequential sera from the patient. All tests showed similarities betweenE. hellemand the nasal isolate but differences between these two isolates andE. cuniculi. Minor protein differences betweenE. hellemand the nasal isolate were not considered sufficient to separate them at the specific level. The new isolate is named the Wainwright isolate ofE. hellem. The ultrastructure of the Wainwright isolatein vitrowas similar to that of the parasitein vivobut there was a greater tendency for disruption of the parasitophorous vacuoles. The deposition of the electrondense surface coat on the sporogonic stages ofE. hellem, as a uniform layer which later thickens, is in contrast to its deposition as broad bands, which later join up, inE. cuniculi. This may be a useful character in distinguishing the species without recourse to analysis of protein profiles.
25
AbdelGhani, Sameh M. M., Ellen Smith Moland, Jennifer A. Black, Nancy D. Hanson, Richard V. Goering, Magdy A. Amine, Amal E. Saafan, Maha Gaafar, Mariam Younan, and Kenneth S. Thomson. "First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt." Journal of Infection in Developing Countries 4, no. 01 (December 14, 2009): 058–60. http://dx.doi.org/10.3855/jidc.386.
Full textAbstract:
Three pediatric isolates of Salmonella serovar Typhimurium from Egypt were reported asCTX-M-14 ESBL producers. Two of the 3 isolates (68 and 111) were 85% related by PFGE;however both were distinct from isolate 94. All three isolates produced multiple clavulanate sensitive β-lactamase bands on IEF with pIs of 7.9 and 5.4 (all isolates), while pI 8.2 enzyme was produced by isolate 94 and pI 7.4 enzyme by isolate 111. The pI 7.9 enzyme was transferable by conjugation and conferred a CTX-M ESBL phenotype. PCR and sequencing identified the CTX-M gene as CTX-M-14. Genes for OXA-1 and SHV-12 were also identified by sequencing. To our knowledge this is the first report of CTX-M-producing Salmonella in Egypt.
26
Liu, Jian Guo, Chang Zhen Chen, and Jing Liu. "A Novel Dialysis Process to Isolate Phosvitin from Hen Egg Yolk." Advanced Materials Research 554-556 (July 2012): 1542–46. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1542.
Full textAbstract:
The objective of this work is to develop a novel dialysis process for the isolation of phosvitin from hen egg yolk avoiding the use of organic solvents and polyvalent metals. This bioseparation process consists of NaCl precipitation, heat treatment and dialysis, which was proposed on the basis of the property difference (especially solubility and thermostability) among yolk proteins. The native molecular mass of the purified phosvitin estimated by fast protein liquid chromatography on a Superdex 75 column was about 165 kDa. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed two bands around 35 kDa. The nitrogen to phosphorus atomic ratio of the purified phosvitin was 2.8 ± 0.2, with a yield of 87.1%. The phosvitin product had α-helix of 36%, β-sheet of 28% and random coil of 36% at pH 7.0, consistent with the literature values. This shows that the purified phosvitin folded with a reasonable secondary structure.
27
Zahara, Ita, Ainun Marliah, and Syamsuddin Syamsuddin. "Identifikasi Kemampuan Bakteri dari Rizosfer Kakao dalam Menghambat Phytophthora palmivora Secara In Vitro." Jurnal Ilmiah Mahasiswa Pertanian 4, no. 1 (March 28, 2020): 53–65. http://dx.doi.org/10.17969/jimfp.v4i1.10289.
Full textAbstract:
Abstrac. Phytophthora palmivora merupakan penyakit yang menyebabkan kerugian besar. Pengendalian terbaik yang harus diupayakan saat ini yaitu menggunakan pengendalian hayati dengan mengisolasi bakteri di daerah rizosfer tanaman kakao. Tujuan dari penelitian ini untuk mendapatkan isolat rizobakteri yang mampu berperan sebagai kadidat agen biokontrol. Penelitian dilaksanakaan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh dari bulan Maret sampai Juni 2018. Rancangan Acak Lengkap (RAL) non Faktorial adalah rancangan yang digunakan dalam penelitian ini dengan parameter pengamatan yang diamati adalah persentase pengahambatan pertumbuhan koloni patogen dan laju pengahambatan pertumbuhan koloni patogen. Daya hambat isolat bakteri yang diperoleh dari rizosfer kakao terbaik ditemukan pada isolat TRIM8/9 dengan persentase 76,67% dan laju hambat 3,16 mm/hari.Identification of the Bacterial Ability of Rizosfer Cocoa in Inhibiting Phytophthora palmivora in In VitroAbstract. Phytophthora palmivora is a disease that causes large losses. The best control that must be sought now is to use biological control by isolating rhizobacteria in the rhizosphere of the cocoa plant. The purpose of this study was to obtain bacterial isolates who were able to act as candidates for biocontrol agents. The research was conducted at the Seed Science and Technology Laboratory of the Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh from March to June 2018. Non Factorial Randomized Complete Design (CRD) was the design used in this study with observed observation variables as percentage inhibition of growth of pathogenic colonies and the rate of inhibition of growth of pathogenic colonies. The inhibition of bacterial isolates obtained from the best cocoa rhizosphere was found in TRIM8/9 isolates with a percentage of 76.67% and inhibition rate of 3.16 mm / day.
28
Erina, Erina, Roslizawaty Roslizawaty, and Sri Wahyuli. "Isolasi Candida sp. dan Aspergilus sp. pada Tembolok (Ingluviens) Ayam Ras dan Ayam Buras di Pasar Peunayong, Banda Aceh." Jurnal Agripet 19, no. 1 (April 1, 2019): 51–58. http://dx.doi.org/10.17969/agripet.v19i1.13162.
Full textAbstract:
ABSTRAK. Penelitian ini bertujuan mengisolasi Candida sp. dan Aspergillus sp. pada tembolok ayam ras dan buras. Sampel dalam penelitian ini adalah tembolok ayam ras dan buras masing-masing berjumlah 15 sampel yang diambil secara acak dari tempat pemotongan unggas Peunayong Banda Aceh. Isolasi Candida sp. dan Aspergillus sp. dilakukan sesuai dengan metode Thompson (1969). Sampel dicuci dengan aquades steril yang diberi antibiotik selanjutnya ditanamkan pada media Sabouraud’s Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 2-7 hari. Pengamatan morfologi Candida sp. dan Aspergillus sp. diamati secara makroskopis. Koloni yang diduga Candida sp. dan Aspergillus sp. diperiksa secara mikroskopis. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan menunjukkan bahwa Candida sp. dapat diisolasi pada semua sampel (100%) tembolok ayam ras dan ayam buras. Aspergillus sp. dapat diisolasi pada 2 dari 15 (13,33%) sampel tembolok ayam ras dan 6 dari 15 (40%) sampel tembolok ayam buras. Kesimpulan penelitian ini adalah Candida sp. tidak ada perbedaan pada tembolok ayam ras dan ayam buras sedangkan Aspergillus sp. pada tembolok ayam buras lebih banyak dari pada ayam ras. (Isolation of Candida sp and Aspergillus sp. from crops (Ingluviens) of broiler and indigenous chicken in Peunayoung market, Banda Aceh) ABSTRACT. This research aimed to isolate Candida sp. and Aspergillus sp. from crop of chicken race (broiler) and indigenous chicken. This research used crops of the chicken race (broiler) and indigenous chicken, each animal consists of 15 animals taken randomly from the poultry of slaughter house Peunayong Banda Aceh. Isolation of Aspergillus sp. was done based on Thompson method (1969). The samples were washed with sterile aquadest containing antibiotics before implanted on Sabouraud’s Dextrose Agar (SDA), then incubated at room temperature for 2-7 days. The plate was observed from Candida sp. and Aspergillus sp. colony macroscopically and microscopically. Data were analyzed descriptively. The result showed that Candida sp. Found in all samples (100%) and Aspergillus sp. Found in 2 out of 15 (13,33%) crops samples in chicken race (broiler) and 6 out of 15 (40%) crops in indigenous chicken. The conclusion is, candida was found in both chickens race (broiler) and domestic chicken, while aspergillus was found more in indigenous chicken than chickens race broiler.
29
Tripoli, Gregory J. "Numerical Study of the 10 January 1998 Lake-Effect Bands Observed during Lake-ICE." Journal of the Atmospheric Sciences 62, no. 9 (September 1, 2005): 3232–49. http://dx.doi.org/10.1175/jas3462.1.
Full textAbstract:
Abstract This paper presents the results of a series of idealized cloud resolving simulations of the evolution of moist roll convection observed as part of the Lake-Induced Convection Experiment (Lake-ICE) that took place during the 1997/98 winter over central Lake Michigan. Satellite and radar observations of the roll convection depict striking linear rolls stretching from 10 km off the western shore of the lake, across to the eastern shore, and then continuing across Michigan. The spacing of the primary rolls was observed to be 6 km, giving a ratio of spacing to depth of about 5:1, which is consistent with theory. In addition, a longer wavelength (13 km) of stationary banding was observed parallel to the shoreline. In an earlier study of this case, multiply nested simulations of the convective rolls based on real data variable initialization were successful in producing banded structures with similar spacing and location over the water to those observed using fine grid resolution of about 500 m. Unfortunately, the initial locations of simulated bands were organized primarily by numerical effects of grid interpolation. This suggested that the spacing of the bands was robust, but that their initial location was highly sensitive to subtle systematic forcings. In this paper, a set of idealized model experiments, designed to isolate the role that physically realistic local forcing plays in the organization of the rolls, was performed. Because externally generated upstream turbulence was suppressed in these tests so as not to bias the result, the generation of rolls was delayed until 20–30 km downwind of the observed location and the location simulated in the previous grid nesting experiments. It was shown that the subtle effects of the shoreline geometry were sufficient to spawn a near-surface streamwise vorticity that became the primary seed for roll development at the most efficient mode of roll convection. These results suggest that previous structures evolved in the upstream shear-driven land-based mixed layer were likely also important in determining where the nonlocal overturning was first triggered. It is not clear from these results whether the shear-driven structures that evolved over the land also played a significant role in organizing the structural geometry of the lake rolls. Results also suggested that the shore parallel bands were a robust feature of the atmospheric structure resulting from resonant gravity wave trapping in the frontal layer.
30
Yu, Yun-Song, Qing Yang, Xiao-Wei Xu, Hai-Shen Kong, Gen-Yun Xu, and Bu-Yun Zhong. "Typing and characterization of carbapenem-resistant Acinetobacter calcoaceticus–baumannii complex in a Chinese hospital." Journal of Medical Microbiology 53, no. 7 (July 1, 2004): 653–56. http://dx.doi.org/10.1099/jmm.0.05513-0.
Full textAbstract:
This study was designed to investigate the prevalence of carbapenem-resistant Acinetobacter calcoaceticus–baumannii complex (Acb complex) and to type carbapenemases. The relatedness of 45 isolates of carbapenem-resistant Acb complex collected from a clinical setting was analysed by PFGE. The carbapenemases produced by these isolates were typed by IEF, a three-dimensional test, 2-mercaptopropanoic acid inhibition assay, PCR and DNA cloning and sequencing. Results showed that all 45 isolates were resistant to multiple antibiotics including meropenem. The resistance rates to cefoperazone/sulbactam and ampicillin/sulbactam were 2.2 and 6.5 %, respectively. About 71.7–78.3 % of these isolates were intermediately resistant to cefepime, ceftazidime and cefotaxime. Forty-five isolates were classified into type A (98 %) and B (2 %) based on their PFGE patterns. Most of type A isolates were from the ICU. Type A was the dominant isolate, including subtypes A1 (22 %), A2 (71 %), A3 (2 %) and A4 (2 %). Only one isolate, from the haematology department, belonged to type B. Forty-three isolates (96 %) were positive for carbapenemase. One isolate had two bands by IEF, the pIs of which were 6.64 and 7.17. The band with the pI of 6.64 was OXA-23. The other 42 isolates produced two bands with pIs of 6.40 and 7.01 which could not be inhibited by clavulanic acid, cloxacillin or 2-mercaptopropanoic acid. It can be concluded that the prevalent carbapenem-resistant Acb complex isolates from this hospital all had similar β-lactamase patterns.
31
Afriyani, Afriyani, Darmawi Darmawi, Fakhrurrazi Fakhrurrazi, Zakiah Heryawati Manaf, Mahdi Abrar, and Winaruddin Winaruddin. "ISOLASI BAKTERI Salmonella sp. PADA FESES ANAK AYAM BROILER DI PASAR ULEE KARENG BANDA ACEH (Isolation of Salmonella sp. in Feces of Broiler Chicks at Ulee Kareng Market Banda Aceh)." Jurnal Medika Veterinaria 10, no. 1 (February 1, 2016): 74. http://dx.doi.org/10.21157/j.med.vet..v10i1.4047.
Full textAbstract:
The research aimed to isolate Salmonella sp.in feces of broiler chicks. Fifteen feces samples of broiler chick were obtained from Ulee Kareng Market in Banda Aceh. Chick cloaca was swabbed using a sterile cotton swab, put into the microtube, and brought to the Microbiology Laboratory of Veterinary Medicine Faculty, Syiah Kuala University. The samples were incubated in selenite cystine broth (SCB) medium, cultured in salmonella shigella agar (SSA) and separated colonies were inoculated in nutrien agar (NA) at 37° C for 24 hours. Bacteria stained with Gram staining then tested biochemically. The result showed that Salmonella sp. were identified in 9 out of 15 feces chicks samples. It ca be concluded that broiler chick in Ulee Kareng Market Banda Aceh contaminated with bacteria Salmonella sp.
32
Ballesté, Elisenda, and Anicet R. Blanch. "Bifidobacterial Diversity and the Development of New Microbial Source Tracking Indicators." Applied and Environmental Microbiology 77, no. 10 (April 1, 2011): 3518–25. http://dx.doi.org/10.1128/aem.02198-10.
Full textAbstract:
ABSTRACTMany studies suggest a close relationship between species ofBifidobacteriumand their hosts. Thus, species such asB. adolescentisandB. thermacidophilumsubsp.porcinumhave been proposed as potential indicators of human and porcine fecal pollution. The diversity of bifidobacteria in wastewaters (human and animal) and slurries is analyzed using nested PCR followed by denaturant gradient gel electrophoresis (DGGE). The sewage samples showed similar DGGE patterns. The predominant bands were recognized asB. adolescentis,B. longum, and two unidentified species related toB. adolescentis. A single band detected in poultry samples was identified asB. saeculare. Bifidobacterial diversity was higher within porcine and bovine samples. The main bands in porcine samples were identified asB. minimum, an unknown species, andB. thermophilum/B. thermacidophilumsubsp.porcinum. The latter species was also identified among the main bands in bovine samples together withB. pseudolongumandB. ruminantium. We then attempted to isolate the host-specific strains. DGGE bands were examined to develop specific probes to screen environmental samples by colony hybridization and further isolation of strains from positively hybridized colonies. Bifidobacterial strains that are host associated by DGGE bands to human and pig were successfully isolated from the environment:B. adolescentisfrom human sewage samples and the unidentified species related to pig from slurries and slaughterhouse wastewater. Neither the poultry-associatedB. saecularenor the ruminant-associatedB. pseudolongumcould be isolated with the current methodology, suggesting either a low prevalence in the samples or failure of the culture to grow in the media used.
33
Forrest, M., J. Isaac-Renton, and W. Bowie. "Immunoblot patterns of Giardia duodenalis isolates from different hosts and geographical locations." Canadian Journal of Microbiology 36, no. 1 (January 1, 1990): 42–46. http://dx.doi.org/10.1139/m90-007.
Full textAbstract:
Eighteen isolates of Giardia duodenalis from animal and human sources were studied for protein differences by polyacrylamide gel electrophoresis and for antigenic differences by immunoblot analysis. The polyacrylamide gels showed that whilst the isolates were for the most part homogeneous in their protein banding patterns, some isolates did show some differences. The immunoblot analysis yielded many bands, including prominent bands of 32 and 66 kilodaltons. Five of the six isolates that showed differences in protein banding pattern also showed differences in antigenic reactivity. Our findings suggest that differences can be seen with the use of immunoblotting and that this technique is a tool that may be useful for isolate differentiation when used in conjunction with other techniques. Key words: Giardia, giardiasis, characterization, immunoblot.
34
Neuville, Segolene, Olivier Lortholary, and Francoise Dromer. "Do Kinetics of the Humoral Response to Cryptococcus neoformans Proteins during Murine Cryptococcosis Reflect Outcome?" Infection and Immunity 68, no. 6 (June 1, 2000): 3724–26. http://dx.doi.org/10.1128/iai.68.6.3724-3726.2000.
Full textAbstract:
ABSTRACT The kinetics of the humoral response to Cryptococcus neoformans proteins were studied in outbred mice infected with isolate NIH52D. Future nonsurvivors had earlier and stronger (i.e., more bands recognized) humoral responses than survivors. In addition, antibodies to a 56- to 60-kDa membrane antigen and to a 39- to 40-kDa cytosolic antigen were detected more frequently in samples from future nonsurvivors and from survivors, respectively (P < 0.05).
35
Simon, A. A., H. H. Kaplan, E. Cloutis, V. E. Hamilton, C. Lantz, D. C. Reuter, D. Trang, S. Fornasier, B. E. Clark, and D. S. Lauretta. "Weak spectral features on (101995) Bennu from the OSIRIS-REx Visible and InfraRed Spectrometer." Astronomy & Astrophysics 644 (December 2020): A148. http://dx.doi.org/10.1051/0004-6361/202039688.
Full textAbstract:
Context. The NASA Origins, Spectral Interpretation, Resource Identification, and Security-Regolith Explorer (OSIRIS-REx) mission has obtained thousands of spectra of asteroid (101955) Bennu with the OSIRIS-REx Visible and InfraRed Spectrometer. Aims. We present a spectral search for minor absorption bands and determine compositional variations on the surface of Bennu. Methods. Reflectance spectra with low and high spatial resolutions were analyzed for evidence of weak absorption bands. Spectra were also divided by a global average spectrum to isolate unique spectral features, and variations in the strongest band depths were mapped on a surface shape model. The global visible to near-IR spectrum of Bennu shows evidence of several weak absorption bands with depths of a few percent. Results. Several observed bands are consistent with phyllosilicates, and their distribution correlates with the stronger 2.74-μm hydration band. A 0.55-μm band is consistent with iron oxides and is deepest in the spectrally reddest areas on Bennu. The presence of hydrated phyllosilicates and iron oxides indicates substantial aqueous alteration in Bennu’s past. Conclusions. Bennu’s spectra are not identical to a limited set of carbonaceous chondrite spectra, possibly due to compositional properties and spatial scale differences; however, returned samples should contain a mixture of common chondrite materials.
36
Adam, R. D., A. Aggarwal, A. A. Lal, V. F. de La Cruz, T. McCutchan, and T. E. Nash. "Antigenic variation of a cysteine-rich protein in Giardia lamblia." Journal of Experimental Medicine 167, no. 1 (January 1, 1988): 109–18. http://dx.doi.org/10.1084/jem.167.1.109.
Full textAbstract:
The WB isolate of Giardia lamblia expresses a cysteine-rich 170-kD surface antigen (CRP170) that undergoes antigenic variation. An (6E7), cytotoxic for isolates expressing CRP170, was used in another study to select antigenic variants from clones of the WB isolate of Giardia. CRP170 was replaced by surface-labeled bands ranging in size from approximately 50 to 170 kD. In this study, mAb 6E7 was used to isolate a 1-kb portion of the CRP170 gene (M2-1) from a lambda gt 11 expression library. The M2-1 clone hybridized to a 5.4-kb transcript from isolates expressing CRP170 but did not hybridize to RNA from antigenic variants. Evidence was found for frequent rearrangements at the CRP170 gene locus. DNA sequencing of the M2-1 clone revealed the presence of long tandem repeats. The putative amino acid sequence of M2-1 reveals a 12% cysteine content, and CRP170 is readily labeled in vivo with cysteine.
37
Howlett, BJ. "Pulsed field gel electrophoresis as a method for examining phylogenetic relationships between organisms; Its application to the genus Phytophthora." Australian Systematic Botany 3, no. 1 (1990): 75. http://dx.doi.org/10.1071/sb9900075.
Full textAbstract:
Pulsed field gel electrophoresis separates chromosomal-sized pieces of DNA in agarose gels and enables karyotype, genome size and genetic maps to be established for organisms where conventional sexual genetics are difficult. This technique has been applied to two isolates of Phytophthora megasperma. In both isolates (numbers 53 and 63), nine chromosomal DNA bands ranging in size from about 1.4 to 4 million base pairs, were separated; the largest band probably consists of several larger DNAs which were unresolved under all conditions tested. Cytological studies by others have shown that isolate 53 has twice the chromosome complement of isolate 63. Since pulsed field gel electrophoresis indicates that chromosomal DNA in both isolates are identical in size distribution, it is likely the ploidy of this isolate is double that of 63. These two isolates can be distinguished by restriction fragment length polymorphisms in the nuclear ribosomal DNA repeat unit. In both isolates the length of this repeat unit is 11 kilobase pairs.
38
Slot, L. A., and K. B. Hendil. "α2-macroglobulin used to isolate intracellular endopeptidases from mammalian cells in culture." Biochemical Journal 255, no. 2 (October 15, 1988): 437–43. http://dx.doi.org/10.1042/bj2550437.
Full textAbstract:
Extracts of cell cultures labelled with [3H]leucine were incubated with human alpha 2-macroglobulin (alpha 2M), a plasma proteinase inhibitor. The proteinase-alpha 2M complexes were then precipitated with immobilized monoclonal antibodies to alpha 2M and analysed by SDS/polyacrylamide-gel electrophoresis. Parallel experiments were done with methylamine-inactivated alpha 2M to check for unspecific binding of cell proteins to alpha 2M. Several 3H-labelled cell proteins bound to active, but not to inactivated, alpha 2M. Such proteins are likely to be proteinases. Putative endopeptidases of subunit Mr 112000, 78,000, 53,000, and in some experiments 88,000 and 16,000, were trapped by alpha 2M in supernatant fractions from IMR90 human fibroblasts, EBTr bovine fibroblasts and HeLa human carcinoma cells. No additional proteins were trapped in the presence of ATP. The Mr-78,000 endopeptidase was identified as calpain II by immunoblotting. At pH 5.3 putative endopeptidases of subunit Mr 80,000, 53,000 and 28,000-32,000 were trapped from IMR90-fibroblast extracts. Immunoblotting showed that both cathepsin B and cathepsin D were present in the Mr-28,000-32,000 electrophoretic bands. The use of alpha 2M and immobilized antibody to alpha 2M thus allows a rapid enrichment of endopeptidases from cell extracts. Some potentials and limitations of the method are discussed.
39
Jin, Bei, Xiaosong Zhou, Bing Li, Caiyan Chen, Xiaosa Zhang, and Siqiao Chen. "Structure and Antioxidant Activity of Soy Protein Isolate-Dextran Conjugates Obtained by TiO2Photocatalysis." BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/150603.
Full textAbstract:
The aim of this study was to investigate the structural characteristics and antioxidant activities of soy protein isolate- (SPI-) dextran conjugates obtained by TiO2photocatalysis treatment. Results revealed that the UV-vis absorption and the fluorescence intensity increased as the photocatalytic power increased (P<0.05). Higher photocatalytic power could promote the extent of glycation and the formation of high molecular weight SPI-dextran conjugates, which were evidenced by free amino group content and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The Fourier transform infrared (FT-IR) spectra suggested that the amide I, II, and III bands of SPI were altered by the glycation induced by TiO2photocatalysis. Moreover, significant changes of secondary structure occurred in SPI-dextran conjugates. Theα-helix,β-sheet,β-turns, and random coil were changed from approximately 10.6%, 37.9%, 12.9%, and 38.6% to 3.8%, 10.4%, 17.7%, and 68.8%, respectively, after treatment at photocatalytic power of 1000 W. In addition, SPI-dextran conjugates obtained by TiO2photocatalysis treatment exhibited high hydroxyl radical scavenging activity and possessed increased reducing power. All data indicated that TiO2photocatalysis was an efficient method for promoting protein-polysaccharide copolymerisation.
40
Gómez-Mascaraque, Laura G., and Samantha C. Pinho. "Microstructural Analysis of Whey/Soy Protein Isolate Mixed Gels Using Confocal Raman Microscopy." Foods 10, no. 9 (September 14, 2021): 2179. http://dx.doi.org/10.3390/foods10092179.
Full textAbstract:
This work explores the potential of confocal Raman microscopy to investigate the microstructure of mixed protein gel systems. Heat-set protein gels were prepared using whey protein isolate (WPI), soy protein isolate (SPI), and mixtures thereof, with a total of five different whey-to-soy protein ratios (100, 75, 50, 25, and 0%). These were analysed using confocal Raman microscopy, and different data analysis approaches were used to maximize the amount of structural and compositional information extracted from the spectral datasets generated, including both univariate and multivariate analysis methods. Small spectral differences were found between pure WPI and SPI gels, mainly attributed to conformational differences (amide bands), but SPI exhibited considerably greater auto-fluorescence than WPI. The univariate analysis method allowed for a rapid microstructural analysis, successfully mapping the distribution of protein and water in the gels. The greater fluorescence of the capsule-like structures found in the mixed gels, compared to other regions rich in proteins, suggested that these may be enriched in soy proteins. Further analysis, using a multivariate approach, allowed us to distinguish proteins with different levels of hydration within the gels and to detect non-proteinaceous compounds. Raman microscopy proved to be particularly useful to detect the presence of residual lipids in protein gels.
41
Adam, R. D., Y. M. Yang, and T. E. Nash. "The cysteine-rich protein gene family of Giardia lamblia: loss of the CRP170 gene in an antigenic variant." Molecular and Cellular Biology 12, no. 3 (March 1992): 1194–201. http://dx.doi.org/10.1128/mcb.12.3.1194.
Full textAbstract:
Giardia lamblia trophozoites demonstrate variable expression of a repertoire of cysteine-rich surface antigens in vitro and in vivo. The size of the repertoire has been estimated at 20 to 184, and specific variants can be detected after approximately 12 generations of in vitro growth for the WB isolate. In earlier studies, we cloned a portion of the gene for a 170-kDa surface antigen (CRP170) and demonstrated by DNA sequencing that it was cysteine rich (12%) and contained 2.6 copies of a tandemly repeated 195-bp pair sequence. The clone hybridized to multiple bands on a Southern blot of G. lamblia DNA in a pattern that was variable among the cloned lines but did not correlate with expression of CRP170. We have now cloned a nearly full length cDNA as well as genomic clones for CRP170 from the WBA6 cloned isolate. In addition, we have isolated a cDNA clone from the WB1269 line (expressing CRP72), an antigenic variant which was derived from WBA6. Sequence analysis of the CRP170 and CRP72 genes revealed marked C-terminal amino acid homology, suggesting a conserved functional role such as membrane anchoring. The CRP170 repeat oligonucleotide hybridized to a stairstep of bands approximately 6 kb in size on HindIII-digested WBA6 DNA representing the expressed copy(ies) of CRP170. In contrast, there was no hybridization to a fragment of similar size in WB1269, suggesting that WB1269 trophozoites have lost the expressed copy of the CRP170 gene.
42
Adam, R. D., Y. M. Yang, and T. E. Nash. "The cysteine-rich protein gene family of Giardia lamblia: loss of the CRP170 gene in an antigenic variant." Molecular and Cellular Biology 12, no. 3 (March 1992): 1194–201. http://dx.doi.org/10.1128/mcb.12.3.1194-1201.1992.
Full textAbstract:
Giardia lamblia trophozoites demonstrate variable expression of a repertoire of cysteine-rich surface antigens in vitro and in vivo. The size of the repertoire has been estimated at 20 to 184, and specific variants can be detected after approximately 12 generations of in vitro growth for the WB isolate. In earlier studies, we cloned a portion of the gene for a 170-kDa surface antigen (CRP170) and demonstrated by DNA sequencing that it was cysteine rich (12%) and contained 2.6 copies of a tandemly repeated 195-bp pair sequence. The clone hybridized to multiple bands on a Southern blot of G. lamblia DNA in a pattern that was variable among the cloned lines but did not correlate with expression of CRP170. We have now cloned a nearly full length cDNA as well as genomic clones for CRP170 from the WBA6 cloned isolate. In addition, we have isolated a cDNA clone from the WB1269 line (expressing CRP72), an antigenic variant which was derived from WBA6. Sequence analysis of the CRP170 and CRP72 genes revealed marked C-terminal amino acid homology, suggesting a conserved functional role such as membrane anchoring. The CRP170 repeat oligonucleotide hybridized to a stairstep of bands approximately 6 kb in size on HindIII-digested WBA6 DNA representing the expressed copy(ies) of CRP170. In contrast, there was no hybridization to a fragment of similar size in WB1269, suggesting that WB1269 trophozoites have lost the expressed copy of the CRP170 gene.
43
Buffone, F. A., D. R. LaBonte, and C. A. Clark. "RAPD Banding Patterns in Sweetpotato Infected with Fusarium lateritium." HortScience 30, no. 4 (July 1995): 912C—912. http://dx.doi.org/10.21273/hortsci.30.4.912c.
Full textAbstract:
Chlorotic leaf distortion is a common disease of sweetpotato caused by Fusarium lateritium. This fungus is unique among Fusarium species in that it grows epiphytically on leaves and shoot tips of sweetpotato. Fusarium lateritium mycelia appear as white masses on leaves, and this fungus can cause chlorosis under periods of bright sunlight. When environmental conditions are not favorable for growth, this organism is not readily observed on sweetpotato. The objective of this research was to see if DNA of F. lateritium is amplified using PCR techniques during amplification of sweetpotato DNA. Our results show cTAB extracts of sweetpotato inoculated with F. lateritium have additional bands not present in a control free of F. lateritium. Furthermore, these bands correspond to banding patterns obtained from the F. lateritium isolate DNA when amplified alone. Researchers who use sweetpotato tissue in PCR-based research, e.g., phylogenetic research, should be aware of these amplified products. This situation is further compounded because numerous F. lateritium biotypes are present in the environment.
44
Marevia, Mayang Sukma, Mauliza Mauliza, and Cut Asmaul Husna. "PERBEDAAN MASALAH PSIKOSOSIAL ANTARA ANAK OBESITAS DAN GIZI NORMAL DI SD NEGERI 1 BANDA SAKTI KOTA LHOKSEUMAWE." AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh 2, no. 2 (February 16, 2018): 41. http://dx.doi.org/10.29103/averrous.v2i2.416.
Full textAbstract:
Obesitas pada anak merupakan peningkatan berat badan melebihi batas kebutuhan skeletal dan fisik akibat akumulasi lemak secara berlebihan dalam tubuh. Salah satu indeks antropometri yang digunakan untuk menentukan status gizi obesitas dan gizi normal adalah Indeks Massa Tubuh (IMT)/umur. Obesitas dan gizi normal masing-masing dikategorikan bila IMT CDC 2000 >P95 dan P5-<P85. Obesitas pada anak berhubungan dengan masalah psikososial seperti penurunan kualitas hidup yang signifikan, risiko lebih besar untuk diintimidasi dan isolasi sosial serta anak menarik diri dari lingkungan sosial. Penelitian bertujuan untuk mengetahui perbedaan masalah psikososial antara anak obesitas dan gizi normal di SD Negeri 1 Banda Sakti Kota Lhokseumawe, menggunakan kuesioner Pediatric Symptom Checklist (PSC)-17. Jenis penelitian bersifat analitik dengan pendekatan cross sectional dan metode purposive sampling dengan total 88 orang (44 anak obesitas dan 44 anak gizi normal. Uji Chi Square menunjukkan terdapat perbedaan masalah psikososial antara anak obesitas dan gizi normal di SD Negeri 1 Banda Sakti Kota Lhokseumawe (p=0,015).
45
Bonoli, C., F. Bonoli, L. Danese, F. Delpino, G. De Zotti, G. Granato, and V. Zitelli. "Multicolor CCD Photometry of a Homogeneous Sample of Seyfert 1 Galaxies." Symposium - International Astronomical Union 134 (1989): 49–50. http://dx.doi.org/10.1017/s0074180900140331.
Full textAbstract:
We have observed a homogeneous sample of Seyfert 1 and 1.5 galaxies with the aim to determine the luminosity function of Seyfert nuclei. Observations were made with the CCD cameras of Asiago and Loiano Observatories in B, V and R bands in order to isolate the contribution of different stellar populations. A photometric decomposition into three components (disc, bulge and nucleus) was fitted to the observed luminosity profiles to separate the nuclear and the host galaxy contributions and to understand whether underlying galaxies actually behave as “normal” galaxies.
46
Soria-Hernández, Cintya G., Sergio O. Serna-Saldívar, and Cristina Chuck-Hernández. "Comparison of Physicochemical, Functional and Nutritional Properties between Proteins of Soybean and a Novel Mixture of Soybean-Maize." Applied Sciences 10, no. 19 (October 8, 2020): 6998. http://dx.doi.org/10.3390/app10196998.
Full textAbstract:
Vegetable proteins are potential low-cost alternatives to solve the protein deficiency of the world population. A protein extracted from a mixture of soybean meal and maize germ was developed to offer more protein alternatives with high nutritional value. In this study, physicochemical, functional, and nutritional characteristics of isolates and hydrolysates of soybean and counterparts extracted from a soybean meal-maize germ were compared. The isolate and hydrolysate of the soybean-maize blend had a protein content of 93.9% and 73.6%, respectively. These protein mixtures contained 10% and 52% more solubility, 303.9%, and 22.7% more emulsifying capacity, 4.5% and 4.2% higher foam density and 36.3% and 1.2% more coagulation capacity compared to the soybean isolate and hydrolysate. Electrophoretic profiles of soybean-maize proteins showed four additional bands to the typical soybean pattern of 56, 55, 52 and 18 kDa, which could correspond to globulins and zeins from maize. The isolate extracted from the mixture of soybean meal and maize is a new alternative to provide the necessary amino acids for proper physical and mental development. Additionally, it has a high potential to be used as an ingredient by the food industry due to its excellent functionality and nutritional value.
47
Mahdani, Wilda, Zinatul Hayati, and Teuku Yusriadi. "PETA DISTRIBUSI DAN RESISTENSI Acinetobacter baumannii DARI SPESIMEN KLINIK DI RSUD DR. ZAINOEL ABIDIN TAHUN 2018." AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh 6, no. 1 (June 25, 2020): 104. http://dx.doi.org/10.29103/averrous.v6i1.2633.
Full textAbstract:
Kemampuan hidup Acinetobacter baumannii pada berbagai keadaan dikombinasikan dengan resistensi berkaitan erat dengan Healthcare Associated Infections (HAIs). Kemunculan strain resisten telah banyak dilaporkan. Penelitian ini bersifat observasional deskriptif. Pemeriksaan spesimen klinik dilakukan di Laboratorium Mikrobiologi Klinik RSUD dr. Zainoel Abidin Banda Aceh. Isolat Acinetobacter baumannii dikumpulkan, dilakukan uji kepekaan serta dinilai tingkat resistensinya. Angka insidensi Acinetobacter baumannii adalah 4,6% yang dominan terisolasi dari spesimen sputum. Strain resisten sangat umum dijumpai pada ruang rawat intensif, ruangan non intensif memiliki lebih banyak strain susceptible. Strain MDR menunjukkan kepekaan terhadap amikacin, trimethoprim-sulfamethoxazole, tobramycin, ampicillin-sulbactam, dan meropenem. Acinetobacter baumannii strain XDR hanya menunjukkan kepekaan yang masih baik terhadap amikacin. Data epidemiologi resistensi antibiotik adalah komponen utama Program Pengendalian Resistensi Antibiotik di rumah sakit
48
Gerk, Andréia O., Elliot W. Kitajima, and Marlinda L. Souza. "Identificação e caracterização de isolado brasileiro do vírus de poliedrose nuclear da lagarta do cartucho-do-milho." Anais da Sociedade Entomológica do Brasil 26, no. 3 (December 1997): 507–15. http://dx.doi.org/10.1590/s0301-80591997000300014.
Full textAbstract:
Foi confirmada a identificação de um vírus de poliedrose nuclear (VPN) de lagartas de Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), coletado de lagartas infectadas em Sete Lagoas, MG. A patologia de lagartas infectadas é característica de VPN. O vírus apresentou tropismo por células da ectoderme, células adiposas e de traqueócitos. Partículas virais (poliedros e vírions liberados por álcali - ARVs) foram purificadas a partir de centrifugações diferenciais em gradientes de sacarose e a banda de poliedros situou-se no terço inferior do tubo. Quanto maior o tempo de digestão dos poliedros, com solução alcalina, maior a dissolução dos corpos de inclusão (poliedros), com consequente liberação de vírions. Houve a formação de cinco bandas nítidas de vírions liberados por álcali. O perfil eletroforético do VPN de S. frugiperda foi comparado ao perfil do VPN de Anticarsia gemmatalis (Hübner), tanto para poliedros como para ARVs. Um peptídeo de 32.000 dalton foi caracterizado como a poliedrina do VPN de S. frugiperda.
49
Bafadal, Mentarry, A. Amaliah Dahlia, and Ahmad Najib. "Isolation and Identification of Free Radical Scavenging Compound from Stem Bark Soursoup (Annona muricata L.) Extract." Jurnal Fitofarmaka Indonesia 7, no. 3 (September 1, 2020): 26–29. http://dx.doi.org/10.33096/jffi.v7i3.656.
Full textAbstract:
Isolation and Identification of free radical active compound from stem bark soursoup (Annona muricata L.) extract. The aim of this research is to observe the chemical compound of stem bark the soursoup (A. muricata L.) which is active as a free radical. 550 gram of stem bark the soursoup (A. muricata L.) was by graduate extracted using n-heksan, ethyl acetat and ethanol solvents respectively. Extract result is the extraction, it was produced 2.4 gram n-heksan extract, 8.4 gram ethyl acetat extract, 2.1 gram ethanolic extract. The isolation of ethyl acetat extract was conducted using colom chromatography (TLC) method using eluent n-hexane : ethyl acetat (7:3) and it was produced four fractions. The third fraction was isolated using preparative thin layer chromatography with mob ile phase n-hexane : ethyl acetat (6:1) and got 2 bands. The isolate identified by UV-Vis spectroscopy, IR spectroscopy and analyzed by chemistry reaction. From the data result show that isolate is acetogenin.
50
Hampson, D. J., J. R. L. Mhoma, and B. Combs. "Analysis of lipopolysaccharide antigens ofTreponema hyodysenteriae." Epidemiology and Infection 103, no. 2 (October 1989): 275–84. http://dx.doi.org/10.1017/s0950268800030636.
Full textAbstract:
SUMMARYLipopolysaccharide (LPS) extracts obtained fromTreponema hyodysenteriaeof serogroups A, B, D and E, and fromT. innocenswere examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), silver-staining, and immunoblotting with hyperimmune rabbit sera. All organisms possessed multiple LPS bands, but their position and number differed.Immunoblotting of LPS with grouping sera identified three or four major antigenic LPS components in the 10-42 kDa range in all organisms: these components were largely specific to each type-organism of a serogroup, and presumably represented group antigens. Although some minor cross-reactivity occurred between LPS from organisms in the different groups, this was insufficient to merit changes to the current LPS serogrouping system for T. hyodysenteriae. Besides this LPS ‘complex’, other higher-molecular-weight material which appeared to be a common component of the treponemes examined was present in low concentrations. Organisms with different serotypes within a serogroup apparently possessed common LPS bands, but also had unique LPS bands which may account for their serotype specificity. One ‘untypable’ organism lacked group-specific LPS and was thought to be a mutant of a group B organism. The loss of serogroup LPS by the isolate suggested that this material is an external component of the cell wall. The availability of an atypical organism lacking LPS components may facilitate further studies on the pathogenesis of swine dysentery.