Academic literature on the topic 'Isolated influence of factors'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Isolated influence of factors.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Isolated influence of factors"
1
Naumenko, O. V., S. G. Danylenko, K. V. Kopylova, and S. M. Gunko. "Influence of Physical-Chemical Factors of Phages Isolated in Dairy Processing Plants of Ukraine." Mikrobiolohichnyi Zhurnal 82, no. 6 (2020): 84–93. http://dx.doi.org/10.15407/microbiolj82.06.084.
Full textAbstract:
When establishing a bacteriophage control system, it is important to introduce new modern approaches to dairy production, including the use of effective, cost-profitable washing and disinfection programs that can provide not only microbiological but also virological safety for production and target products. At the same time, information on reliable anti-phage treatment in dairy processing plants is extremely limited. Aim. Investigation of the virucidal activity of some disinfectants, depending on the composition, treatment conditions and titer of phage contamination. Methods. The objects of the study were virulent phages F 11; F/2 of Lactococcus lactis ssp., isolated in dairy processing plants from the collection of the Institute of Food Resources of the NAAS of Ukraine; disinfectants approved for use in the dairy industry (LLC “Lizoform”, Kyiv). The virucidal activity of the disinfectants was evaluated by the difference between the phage titer values in sterile distilled water without and with the addition of the disinfectant after a certain treatment time. The sensitivity of phages to the effect of the disinfectant was characterized by a constant of inactivation (Cin). The presence of active phages was determined by the “double agar” method with the addition of 10 mmol·l-1 CaCl2, 100 mmol·l-1 glycine. Results. Screening of physical and chemical factors that inhibit the development of virulent phages F 11 and F/2 of Lactococcus lactis ssp. isolated in dairy processing plants of Ukraine was performed. It was found that the most detrimental effect on these phages had disinfectants with such active substances as peracetic acid (PA), quaternary ammonium compounds (QAC), and active chlorine (Cl2). It was determined that the minimum inhibitory concentrations of active chemical substance during “cold disinfection” at a temperature 20–22ºС were sufficiently high: for PA – 500–2000 mg·l-1 (p≤0.05); QAC – 900–1000 mg·l-1; Cl2 – 800–1000 mg·l-1, p≤0.01. Comparison of the phage inactivation rate with respect to the initial contamination level showed that phages in high titer 108 PFU·ml-1 (the most dangerous, critical level of contamination) were more resistant to treatment than phages in medium titer 106 PFU·ml-1. It was shown that the investigated phage F11 of Lactococcus lactis (936 species) were characterized by greater resistance to disinfectants compared to the phage F/2 of Lactococcus lactis (с2 species). Conclusions. The conditions of anti-phage treatment are experimentally substantiated. It is established that the effectiveness of disinfection depends on the type and concentration of the active chemical substance, as well as on the content and properties of phages that circulate in dairy processing plants of Ukraine.
2
Mahmood, Aseel I., and Mohammed Shehab Abo-Ksour. "Influence of Helium-Neon Laser on Some Virulence Factors of Staphylococcus Aureus and Escherichia Coli." Al-Mustansiriyah Journal of Science 29, no. 3 (2019): 29. http://dx.doi.org/10.23851/mjs.v29i3.619.
Full textAbstract:
This work aims to investigate the effect of Helium-Neon laser on locally isolated Staphylococcus aureus and Escherichia coli bacteria; their resistance pattern, virulence factors, and their plasmid content. Bacteria were collected from patient suffering from urinary tract infections, septicemia, wound, and burn infections, then isolates were identified according to their characteristics, features; morphological, biochemical tests, and API systems. Out of Eighty-seven samples, twenty-seven isolates of S. aureus and thirteen isolates of E. coli were isolated. The results showed that after laser treatment, the diameter of inhibition zone increased for almost of the used antibiotics beside some isolates became sensitive especially after 2 min of laser exposure time. The hemolysin production was disappeared in (%40) of E. coli and (%20) of S. aureus after ten min of irradiation, while it decreased to half in another S. aureus isolate. All S. aureus and E. coli isolates loosed their ability to produce β-lactamase enzymes; some of these isolates loosed it after 5 minutes and the others after 10 minutes of irradiation. The results showed that laser irradiation hasn't any effect at any exposure time on adhesion factors of both S. aureus and E. coli isolates. Plasmid profile of Irradiated E. coli illustrated disappearing of DNA plasmid bands as well as RNA after ten min of irradiation.
3
Silva, Vanessa, José Eduardo Pereira, Luís Maltez, Patrícia Poeta, and Gilberto Igrejas. "Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water." Pathogens 11, no. 10 (2022): 1069. http://dx.doi.org/10.3390/pathogens11101069.
Full textAbstract:
The presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus species survive in the natural environment due to their tolerance to a wide range of temperatures, dryness, dehydration, and low water activity. Therefore, we aimed to evaluate the influence of external environmental factors on the formation of biofilm of staphylococci isolated from hospital wastewater and surface waters. We investigated the biofilm formation of methicillin-resistant and -susceptible S. aureus (MRSA and MSSA) and coagulase-negative staphylococci (CoNS) under various temperatures, pH values, salt concentrations, glucose concentrations, and under anaerobic and aerobic conditions. CoNS had the ability to produce more biofilm biomass than MSSA and MRSA. All environmental factors studied influenced the biofilm formation of staphylococci isolates after 24 h of incubation. Higher biofilm formation was achieved at 4% of NaCl and 0.5% of glucose for MSSA and CoNS, and 1% of NaCl and 1.5% of glucose for MRSA isolates. Biofilm formation of isolates was greater at 25 °C and 37 °C than at 10 °C and 4 °C. pH values between 6 and 8 led to more robust biofilm formation than pH levels of 9 and 5. Although staphylococci are facultative anaerobes, biofilm formation was higher in the presence of oxygen. The results demonstrated that multiple environmental factors affect staphylococci biofilm formation. Different conditions affect differently the biofilm formation of MRSA, MSSA, and CoNS strains.
4
Jiang, L. W., and M. Schindler. "Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study." Journal of Cell Biology 102, no. 3 (1986): 853–58. http://dx.doi.org/10.1083/jcb.102.3.853.
Full textAbstract:
The technique of fluorescence redistribution after photobleaching was used to measure the translocation rate of fluorescein-labeled dextrans across the nuclear pore complex in isolated rat liver nuclei. A transport assay system was established that could monitor the effect of biologically active molecules, e.g., ATP, GTP, cAMP on the translocation process. The results show that ATP, phosphoinositides, RNA, and insulin can enhance transport rates from 195 to 432%. It was further demonstrated that concanavalin A, but not wheat germ or soybean agglutinin, can block dextran transport completely. The effectors of dextran transport are similar to substances demonstrated to effect the efflux of RNA from isolated nuclei. A model for translocation through the nuclear pore is now presented that incorporates data from protein influx and RNA efflux experiments into a single pathway controlled by ATP.
5
Martinez-Urtaza, Jaime, Montserrat Saco, Jacobo de Novoa, et al. "Influence of Environmental Factors and Human Activity on the Presence of Salmonella Serovars in a Marine Environment." Applied and Environmental Microbiology 70, no. 4 (2004): 2089–97. http://dx.doi.org/10.1128/aem.70.4.2089-2097.2004.
Full textAbstract:
ABSTRACT The temporal and spatial distribution of Salmonella contamination in the coastal waters of Galicia (northwestern Spain) relative to contamination events with different environmental factors (temperature, wind, hours of sunlight, rainfall, and river flow) were investigated over a 4-year period. Salmonellae were isolated from 127 of 5,384 samples of molluscs and seawater (2.4%), and no significant differences (P < 0.05) between isolates obtained in different years were observed. The incidence of salmonellae was significantly higher in water column samples (2.9%) than in those taken from the marine benthos (0.7%). Of the 127 strains of Salmonella isolated, 20 different serovars were identified. Salmonella enterica serovar Senftenberg was the predominant serovar, being represented by 54 isolates (42.5%), followed by serovar Typhimurium (19 isolates [15%]) and serovar Agona (12 isolates [9.4%]). Serovar Senftenberg was detected at specific points on the coast and could not be related to any of the environmental parameters analyzed. All serovars except Salmonella serovar Senftenberg were found principally in the southern coastal areas close to the mouths of rivers, and their incidence was associated with high southwestern wind and rainfall. Using multiple logistic regression analysis models, the prevalence of salmonellae was best explained by environmental parameters on the day prior to sampling. Understanding this relationship may be useful for the control of molluscan shellfish harvests, with wind and rainfall serving as triggers for closure.
6
Zhang, Peipei, József Baranyi, and Mark Tamplin. "Interstrain Interactions between Bacteria Isolated from Vacuum-Packaged Refrigerated Beef." Applied and Environmental Microbiology 81, no. 8 (2015): 2753–61. http://dx.doi.org/10.1128/aem.03933-14.
Full textAbstract:
ABSTRACTThe formation of bacterial spoilage communities in food is influenced by both extrinsic and intrinsic environmental factors. Although many reports describe how these factors affect bacterial growth, much less is known about interactions among bacteria, which may influence community structure. This study investigated interactions among representative species of bacteria isolated from vacuum-packaged (VP) beef. Thirty-nine effectors and 20 target isolates were selected, representing 10 bacterial genera:Carnobacterium,Pseudomonas,Hafnia,Serratia,Yersinia,Rahnella,Brochothrix,Bacillus,Leuconostoc, andStaphylococcus. The influence of live effectors on growth of target isolates was measured by spot-lawn agar assay and also in liquid culture medium broth using live targets and effector cell-free supernatants. Inhibition on agar was quantified by diameter of inhibition zone and in broth by measuring detection time, growth rate, and maximum population density. A number of interactions were observed, with 28.6% of isolates inhibiting and 4.2% promoting growth. The majority ofPseudomonasisolates antagonized growth of approximately one-half of target isolates. TwoBacillusspp. each inhibited 16 targets. Among lactic acid bacteria (LAB),Carnobacterium maltaromaticuminhibited a wider range of isolates compared to other LAB. The majority of effector isolates enhancing target isolate growth were Gram-negative, includingPseudomonasspp. andEnterobacteriaceae. These findings markedly improve the understanding of potential interactions among spoilage bacteria, possibly leading to more mechanistic descriptions of bacterial community formation in VP beef and other foods.
7
Ma, Yu Hong, Gui Feng Zhao, and Rui Hai Chen. "Earthquake Damage Prediction of Base-Isolated Structures Based on Fuzzy Comprehensive Evaluation Method." Advanced Materials Research 446-449 (January 2012): 2964–72. http://dx.doi.org/10.4028/www.scientific.net/amr.446-449.2964.
Full textAbstract:
Earthquake damage prediction of base-isolated structure includes many fuzzy factors, for example damage grade, intensity and so on, so by use of the fuzzy comprehensive evaluation method to predict the earthquake damage probability of the base-isolated structure should be feasible and effective. The isolated building usually consist of the isolated layer and the upper structure, so the damage grade of the base-isolated structure is divided by the performances of the isolated layer and the supper-structure. And then, the basic frame system of earthquake damage prediction based on fuzzy comprehensive evaluation method is established: the damage influence factors of the earthquake damage prediction for the isolated structure are analyzed, the corresponding hierarchy evaluations system is presented. Grade standard of damage influence factors and the determining method of membership degree for the base-isolated frame structure are given, and then the weight coefficients of the damage influence factors are studied by use of the judgment matrix analysis method, the fuzzy comprehensive evaluation model of earthquake damage prediction is given. Lastly, a numeral example is analyzed, the rationality of the method is proved.
8
Dereli, S., H. Fleck, J. Sieradzki, and H. Schatz. "Influence of growth factors on an insulin-producing cell line (RINm5F)." Acta Endocrinologica 118, no. 4 (1988): 559–65. http://dx.doi.org/10.1530/acta.0.1180559.
Full textAbstract:
Abstract. The influence of IGF-I, insulin and epidermal growth factor (EGF) as well as glucose as control was studied on both growth and function of RINm5F cells, an insulin-producing cell line derived from rat insulinoma tissue. [3H]thymidine incorporation and DNA content (fluorometrically) were measured for estimating cell growth, insulin release and biosynthesis ([3H]leucine incorporation) as parameters for cell function. There was a significant increase in both [3H] thymidine incorporation and DNA content into RINm5F cells by glucose already at very low concentrations. IGF-I and high concentrations of insulin too were able to stimulate cell growth (insulin also in additional experiments with isolated rat islets). EGF, however, was without growth effect on this cell line (in contrast to previous own results in isolated islets). Insulin secretion and biosynthesis were also increased by very low glucose concentrations. IGF-I and EGF were ineffective regarding these functional parameters in RINm5F cells. Besides for glucose, our results demonstrate a role for IGF-I and insulin, but not for EGF, in regulating growth of these cells.
9
Nguyen, Hien Thi. "ISOLATION, SCREENING AND THE INFLUENCE OF CULTIVATION FACTORS ON CELLULASE OF BACTERIA ISOLATED FROM TERMITES GUT." Vietnam Journal of Science and Technology 54, no. 4A (2018): 89. http://dx.doi.org/10.15625/2525-2518/54/4a/11982.
Full textAbstract:
From five termites guts samples collected in different places in Ha Noi and Vinh, 11 isolates which formed halo on agar plates with CMC were isolated. Among them strain G4 possessed the highest CMCase activity. Based on morphology and 16S rRNA gene sequences analysis, G4 was identified as Bacillus subtilis G4. The results from cultivation study revealed that M3 medium containing rice bran 1 %, soy flour 1 %, casein 1 % and 1 % NaCl was the best medium for cellulase production of B. subtilis G4. At optimal conditions for G4 which were inoculums ratio 1 %, cultivation temperature 37ºC, cultivation time 72 hours, shaking speed 150 rpm and initial pH medium 7, CMCase and FPase activity reached 3.36 U/ml and 0.35 U/ml, respectively.
10
Sánchez, Beatriz, Antonio Cobo, Marina Hidalgo, et al. "Influence of the Type of Diet on the Incidence of Pathogenic Factors and Antibiotic Resistance in Enterococci Isolated from Faeces in Mice." International Journal of Molecular Sciences 20, no. 17 (2019): 4290. http://dx.doi.org/10.3390/ijms20174290.
Full textAbstract:
A comparative study on potential risks was carried out in a collection of 50 enterococci isolated from faeces of mice fed a standard or a high-fat diet enriched with extra virgin olive oil, refined olive oil or butter, at the beginning, after six weeks and after twelve weeks of experiments. Strains were biochemically assessed and genetically characterized. E. faecalis and E. casseliflavus were the most frequently isolated species in any diet and time points. Apart from the fact of not having isolated any strain from the virgin olive oil group during the last balance, we found statistically significant differences p < 0 . 05 among the diets in the percentage of antibiotic resistance and in the presence of the enterococcal surface protein gene (esp), as well as a tendency p < 0 . 1 for the presence of the tyrosine decarboxylase gene (tdc) to increase over time in the group of isolates from the standard diet. When the resistance of the strains to virgin or refined olive oil was studied, only the group of enterococci from high fat diets showed a significantly higher percentage of resistance to refined olive oil p < 0 . 05 , while both types of oil equally inhibited those isolated from the standard diet p > 0 . 05 .
Dissertations / Theses on the topic "Isolated influence of factors"
1
Reyhanogullari, Naim Eser. "Vertical Ground Motion Influence On Seismically Isolated &." Master's thesis, METU, 2010. http://etd.lib.metu.edu.tr/upload/2/12611785/index.pdf.
Full textAbstract:
In this study influences of vertical ground motion on seismically isolated bridges were investigated for seven different earthquake data. One assessment of bearing effect involves the calculation of vertical earthquake load on the seismically isolated bridges. This paper investigates the influence of vertical earthquake excitation on the response of briefly steel girder composite bridges (SCB) with and without seismic isolation through specifically selected earthquakes. In detail, the bridge is composed of 30m long three spans, concrete double piers at each axis supported by mat foundations with pile systems. At both end of the spans
there exists concrete abutments to support superstructure of the bridge. SCBs which were seismically isolated with nine commonly preferred different lead&<br>#8208<br>rubber bearings (LRB) under each steel girder were analyzed. Then, the comparisons were made with a SCB without seismic isolation. Initially, a preliminary design was made and reasonable sections
for the bridge have been obtained. As a result of this, the steel girder bridge sections were checked with AASHTO provisions and analytical model was updated accordingly. Earthquake records were thought as the main loading sources. Hence both cases were exposed to tri&<br>#8208<br>axial
earthquake loads in order to understand the effects under such circumstances. Seven near fault earthquake data were selected by considering possession of directivity. Several runs using the chosen earthquakes were performed in order to be able to derive satisfactory comparisons between different types of isolators. Analytical calculations were conducted using well known structural analysis software (SAS) SAP2000. Nonlinear time history analysis was performed using the analytical model of the bridge with and without seismic isolation. Response data collected from SAS was used to determine the vertical load on the piers and middle span midspan moment on the steel girders due to the vertical and horizontal component of excitation. Comparisons dealing with the effects of horizontal only and horizontal plus vertical earthquake loads were introduced.
2
Higazy, M. G. A. F. I. "Influence of isolated backward-facing steps on boundary layers." Thesis, University of Leicester, 1986. http://hdl.handle.net/2381/34778.
Full textAbstract:
The drag of backward-facing steps was experimentally measured in a series of turbulent boundary layer flows and in a laminar flow. The main objective of the investigation was to study the influence of the freestream pressure gradients on the drag values for backward-facing steps in turbulent boundary layer flows. The measured boundary layer characteristics of all the established flows are in very good agreement with other published results and their two-dimensionality was ascertained. Two different techniques have been used to measure the drag of such steps. The momentum thickness technique was not suitable for determining the drag of such small irregularities, since the incremental increase in the momentum thickness due to the irregularities was small and the method of calculation was crude. The pressure distribution technique was found to be a more suitable method of estimating this drag. When applied appropriately the measurement uncertainty was small. The drag results published by Gaudet and Johnson for backward-facing steps in a zero-pressure gradient have been considerably extended for a number of turbulent boundary layer flows with values of pressure gradient parameter, a, from -0.97 X 10-3 to 9.14 X 10-3. For small steps results covered a range of irregularity Reynolds number of 150 to 900. For large steps in zero-pressure gradient flow this range was increased up to 3500, and up to 2000 in a mild adverse pressure gradient flow. The drags of backward-facing steps in a laminar boundary layer flow on a flat plate have been measured by the pressure distribution technique. No correlation was made for the drag of such steps in the laminar boundary layer, however a comparison between the total drag of the plate with and without the largest step showed that the total drag of the plate increased by only some 65 % of the clean surface drag value.
3
MacLennan, Stephen John. "The influence of oxygen on the isolated human umbilical artery." Thesis, University of Glasgow, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329509.
Full text
4
Chang, Lynda. "Molecular characterisation of GnRH regulated factors isolated from gonadotroph cells." Thesis, University of Edinburgh, 2002. http://hdl.handle.net/1842/30448.
Full textAbstract:
The aim of this thesis was to isolate the factors regulated by GnRH. To identify these, the LβT2 gonadotroph cell line was used as a model system for differential display RT-PCR analysis (DD-RT-PCR) of GnRH regulated transcripts. DD-RT-PCR showed that varying the GnRH pulse regime both differentially regulated and induced rapid changes in mRNA transcript levels. Myosin light chain and a putative tyrosine phosphatase were up regulated by GnRH, as were two expressed sequence tags (ESTs); one expressed in the thesis, the other in the mammary glands of pregnant or lactating mice. In addition, GnRH also down-regulated Fanconi’s anaemia complementation group A (FAA) mRNA expression levels. Fanconi’s anaemia (FA) is a autosomal recessive human disease, characterised by aplastic anaemia, short stature, developmental abnormalities, microcephaly, and infertility. Most FA genetic abnormalities map to the <i>FAA</i> gene, which may have a role in DNA repair, and cell cycle checkpoint control. A role for FAA in controlling gonadotrophin gene expression was tested by transient transfection assay in gonadotroph cell-lines. Transfection of a FAA expression construct specifically repressed the GnRH response of the αGSU promoter, but not the LHβ gene promoter, in LβT2 cells. The regulatory region of the FAA protein, responsible for repression of αGSU, was mapped between amino acid (aa) residues 322aa and 800aa, by serial transfection of successive C-terminal deletion constructs. Furthermore, results also suggested that FAA might mediate its effects through a paired homeodomain binding site on the αGSU promoter. Therefore, using DD-RT-PCR to isolate transcripts from GnRH treated LβT2 cells has identified novel transcripts, transcripts encoding secretion and second messenger signalling pathway proteins and FAA. Furthermore, a novel role for FAA in specifically repressing GnRH-regulated αGSU transcription was discovered.
5
Moore, Ryan A. "ISOLATED MITRAL VALVE ENDOCARDITIS: PATIENT,DISEASE, AND SURGICAL FACTORS INFLUENCINGOUTCOMES." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1623424971098889.
Full text
6
Moeller, Mary. "Factors That Influence Peer Grading." Ashland University Honors Theses / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=auhonors1430752119.
Full text
7
Sun, Hongmei. "Factors that influence chelate cooperativity." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/6583/.
Full text
8
Kotala, Justin E. "Influence of Lactobacillus rhamnosus Isolated from “Amabere Amaruranu” Cultured Milk on Adipogenesis." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etd/2608.
Full textAbstract:
This study was performed to test the in vitro effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu”, a traditional Kenyan cultured milk, on 3T3-L1 and Caco-2 cell lines. Cultures of fully mature 3T3-L1 adipocytes were treated with bacterial isolate cell extract (CE), filtered spent broth (FSB) from overnight bacterial culture, or with a PBS control. Expression levels of PPAR³1 and 2, C/EBP±, and ATGL proteins in 3T3-L1 cells were upregulated by FSB treatment. CE treatment did not affect protein expression levels. Expression of MTTP and SREBP-1c proteins in Caco-2 cells showed no change with either treatment. Optical density measurements from Oil-Red-O stained 3T3-L1 adipocytes increased from PBS control cells to 25μl/ml FSB treated cells; measurements were reduced by treatments above 25μl/ml FSB. In conclusion, filtered spent broth prepared from a culture of Lactobacillus rhamnosus, isolated from “amabere amaruranu” cultured milk showed PPAR³1 and 2, C/EBP±, and ATGL agonistic properties.
9
Lumkwana, Dumisile. "Identifying appropriate attachment factors for isolated adult rat cardiomyocyte culture and experimentation." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86479.
Full textAbstract:
Thesis (MScMedSc)--Stellenbosch University, 2014.<br>ENGLISH ABSTRACT: Introduction: Primary culture of isolated adult rat cardiomyocytes (ARCMs) is an important model for cardiovascular research, but successful maintenance of these cells in culture for their use in experiments remains challenging (Xu et al, 2009; Louch et al, 2011). Most studies are done on acutely isolated cardiomyocytes immediately after isolation, which is due to low survival of these cells in culture. Obstacles in culture are due to the type of medium and attachment factors (tissue culture adhesives) used to culture and grow these cells. Although we previously identified an optimum medium and adhesive for culture, an adhesive that permits cells to remain attached to the culture surface until after an ischemia/reperfusion insult was elusive.
Aims: We therefore aimed to identify the best attachment factor and concentration that will allow adult rat cardiomyocytes to remain attached to the culture surfaces after ischemia/reperfusion experiments. Methods: Cardiomyocytes were isolated from adult Wistar rat hearts and cultured overnight on different concentrations (25 -200 μg/ml) of collagen 1, collagen 4, extracellular matrix (ECM), laminin/entactin (L/E) and laminin. Following overnight cultures, experiments were done in PBS and in PBS versus MMXCB to compare ARCM attachment and viability. Cardiomyocytes cultured on ECM, L/E and L (25−200μg/ml) were subjected to 1 hour of simulated ischemia using MMXCB that contained 3mM SDT and 10mM 2DG, followed by 15 minutes reperfusion. Cell viability was determined by staining cells with JC-1 and images of cells in a field view of 1.17μm/mm2 were captured using fluorescence microscopy. The cells were analysed according to morphology and fluorescence intensity.
Results: Total and rod-shaped ARCMs attachment was improved when MMXCB was used as an experimental buffer instead of PBS. Regardless of the buffer used, morphological viability was poor on substrates of Col 1 and Col 4. In contrast to collagens, ARCMs attached efficiently and morphological viability was high on substrates of ECM, L/E and L in MMXCB, but this was greatly reduced in PBS. Mitochondrial viability was high in MMXCB compared to PBS on Col 1 and Col 4 at 75−175μg/ml and on ECM, L/E and L at all concentrations, except at 50 and 150μg/ml ECM, 175μg/ml L/E and 25μg/ml L.
When cardiomyocytes cultured on ECM, L/E and L were subjected to simulated ischemia, total ARCMs, rod-shaped and R/G fluorescence (mitochondrial viability) was reduced at all concentrations compared to the control group. Hypercontracted cells were higher in the ischemic treated cells compared to the controls on ECM at 75−150μg/ml and 200μg/ml, L/E at 50,100μg/ml and 175μg/ml and on L at 125μg/ml. Total numbers of ARCMs attached on ECM, L/E and L in the ischemic group consisted of similar numbers of non-viable hypercontracted and viable rod-shaped cells.
Conclusion: Cardiomyocytes should be cultured on ECM or L/E or L at concentrations from 25−200μg/ml in MMXCB. PBS is harmful to cultured ARCMs and should thus not be used as an experimental buffer. Ischemia/reperfusion can be simulated on ARCMs cultured on ECM, L/E or L from 25−200μg/ml, provided that a modified culture buffer is used as experimental buffer.<br>AFRIKAANSE OPSOMMING: Inleiding: Primêre selkulture van geïsoleerde volwasse rot kardiomiosiete (VRKMe) is ‘n belangrike model vir kardiovaskulêre navorsing, maar om hierdie selle suksesvol in kultuur te onderhou is ‘n groot uitdaging (Xu et al, 2009; Louch et al, 2011). Die meeste navorsingstudies maak gebruik van akuut geïsoleerde kardiomiosiete onmiddelik na isolasie omdat oorlewing van hierdie selle in kultuur baie laag is. Die struikelblokke in kultuur is as gevolg van die tipe medium en weefselkultuurgom wat gebruik word. Ons het voorheen 'n optimale medium en weefselkultuurgom geïdentifiseer vir VRKM kultuur oorlewing, maar die weefselkultuurgom was nie effektief genoeg om die selle aan die kultuuroppervlak te laat bly vaskleef, tot na die einde van 'n isgemie/herperfusie eksperiment nie.
Doel: Die doel was dus om die beste weefselkultuurgom en konsentrasie te identifiseer, wat sal toelaat dat VRKMe verbonde bly aan die kultuuroppervlaktes tot na die einde van isgemie/herperfusie eksperimente. Metodes: Kardiomiosiete was geïsoleer vanaf volwasse Wistar rotharte en oornag in kultuur op verskillende konsentrasies (25 -200 μg/ml) van kollageen 1, kollageen 4, ekstrasellulêre matriks (ESM), laminin/entactin (L/E) en laminin onderhou. Die volgende dag was die VRKMe vir eksperimentasie in PBS en in PBS teenoor MMXCB gebruik, om selbehoud en oorlewing te vergelyk. Kardiomiosiete op ESM, L/E en L (25−200μg/ml) was aan 1 uur van gesimuleerde isgemie blootgestel, in MMXCB wat 3mM SDT en 10mM 2DG bevat het, gevolg deur 15 minute herperfusie. Sel oorlewing was bepaal deur selle te kleur met JC-1 en daarna was fluoressensiebeelde van die selle in ‘n veldgebied van 1.17μm/mm2 geneem. Die selle was volgens selmorfologie en fluoressensie intensiteit ontleed.
Resultate: Met die gebruik van MMXCB as eksperimentele buffer in plaas van PBS, het die aantal totale en staafvormige VRKMe verbinding verbeter. Morfologiese onderhoud was sleg op kollageen 1 en 4, ongeag van watter buffer gebruik was. In kontras met die kollagene was die VRKM verbinding en morfologiese onderhoud op ESM, L/E en L in MMXCB effektief verbeter, maar in PBS aansienlik verminder. Mitochondriale lewensvatbaarheid in MMXCB teenoor PBS op kollageen 1 en 4 by 75−175μg/ml, sowel as op ECM, L/E en L by alle konsentrasies, was hoog, behalwe by 50 en 150μg/ml ESM, 175μg/ml L/E en 25μg/ml L.
Isgemie blootstelling van kardiomiosiete gekultuur op alle konsentrasies van ESM, L/E en L, het ‘n afname in die totale, staafvormige en R/G fluoressensie (mitochondriale lewensvatbaarheid) teweeggebring. Meer hiperkontrakteerde kardiomiosiete was in die isgemie behandelde groepe as in die kontrole groepe teenwoordig, spesifiek op ESM by 75−150μg/ml en 200μg/ml, op L/E by 50,100μg/ml en 175μg/ml asook op L by 125μg/ml. In die isgemie groepe het die totale aantal VRKMe op ESM, L/E en L meestal uit ‘n gelyke hoeveelheid hiperkontrakteerde en staafvormige selle bestaan.
Gevolgtrekking: Kardiomiosiete moet op ESM of L/E of L by konsentrasises van 25−200μg/ml in MMXCB gekultuur word. PBS is nadelig vir VRKMe in kultuur en moet dus nie gebruik word as eksperimentele buffer nie. Isgemie/herperfusie eksperimente kan gesimuleer word op VRKMe wat op 25−200μg/ml ESM, L/E of L gekultuur is, mits ‘n gemodifiseerde kultuur buffer gebruik word as eksperimentele buffer.
10
Silva, Camila MagalhÃes. "Identification phenotypic, genotypic virulence factors and characterization in isolated environmental Aeromonas spp." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14414.
Full textAbstract:
This study aimed to identify through polyphasic approach (phenotypic and genotypic methods) strains of Aeromonas spp. isolated from surface water and sediment from three different points of the Coco River, Cearà following the salinity gradient of the water. Sampling was conducted between October 2011 and March 2012 each collection consisted of six samples. Aeromonas strains were isolated according to the technique described in the literature Gelatin Phosphate Salt Agar (agar plus GSP 20 μg/mL ampicillin). The physico-chemical parameters (salinity, pH and temperature) of water were on a track that favored not only survival, but also the multiplication of micro-organisms in both sites. Among this sample was isolated 140 strains suspected of Aeromonas and (36.4%) 51 strains were confirmed like Aeromonas spp., wich will be identifiyed until species for fenotipic and genotipic tests. In parallel to the insulation work was done a survey of alternative culture media, where we assessed the efficiency of culture media with different compositions in the characterization of colonies of multiple species of Aeromonas. While the antimicrobial susceptibility profile of Aeromonas strains were resistant 100% penicillin (PEN) and cephalothin (CFL) and 100% antimicrobial susceptibility Amikacin (AMI) and imipenem (IMP) among the nine antimicrobials tested and generally A. caviae was the species that showed resistance to antimicrobials. Among the 27 strains tested (45.8%) were multidrug-resistant by making a multiple resistence antibiotic (MRA) > 2. Five factors of different virulence and all strains were tested had at least one of the five virulence factors tested so that different combinations of virulence factors were found in isolates from the same sample. The results of genetic analysis using primers Aero16S-F and Aero16S-F showed that all isolates identified by conventional microbiological tests were confirmed by PCR and 65.6% of the species identified by conventional biochemical tests were confirmed by Box-PCR method.<br>Este trabalho teve como objetivo principal identificar atravÃs de abordagem polifÃsica (tÃcnicas fenotÃpicas e genotÃpicas) de cepas de Aeromonas spp. isoladas de Ãgua de superfÃcie e sedimento de trÃs pontos distintos do Rio CocÃ, Fortaleza, Cearà seguindo o gradiente de salinidade da Ãgua. Foram realizadas coletas no perÃodo de outubro de 2011 a marÃo de 2012, sendo cada coleta composta por seis amostras. As cepas de Aeromonas foram isoladas de acordo com a tÃcnica descrita na literatura em Ãgar Gelatina Fosfato Sal (Ãgar GSP acrescido de 20 μg/mL de ampicilina). Os parÃmetros fÃsico-quÃmicos (salinidade, pH e temperatura) da Ãgua estavam em uma faixa que favorecia nÃo sà a sobrevivÃncia, mas tambÃm a multiplicaÃÃo dos micro-organismos em dois pontos de coleta. Foram isoladas 140 cepas suspeitas de Aeromonas, 36 (25,7%) foram identificadas atà espÃcie sendo elas: A. caviae, A. media, A. eucrenophila e A. veronii, sendo A. caviae a espÃcie que apareceu com maior frequÃncia. Em paralelo ao trabalho de isolamento foi feita uma pesquisa com dois meios de cultivo alternativos, Ãgar UNISC e Ãgar Amido Ampicilina (AAA), nos quais foi avaliada a eficiÃncia dos meios de cultura com diferentes composiÃÃes, na caracterizaÃÃo de colÃnias de vÃrias espÃcies de Aeromonas. O perfil de suscetibilidade antimicrobiana das cepas de Aeromonas apresentou resistÃncia de 100% a Penicilina (PEN) e Cefalotina (CFL) e 100% de sensibilidade aos antimicrobianos Amicacina (AMI) e Imipenem (IMP) entre os nove antimicrobianos testados e de forma geral A. caviae (38,9%) foi a espÃcie que mais apresentou resistÃncia. Dentre as cepas avaliadas 27 (45,8%) foram multirresistentes por apresentarem um MAR > 2. Foram testados cinco fatores de virulÃncia diferentes e todas as cepas apresentaram pelo menos um desses fatores, de forma que diferentes combinaÃÃes desses fatores foram observadas em isolados provenientes da mesma amostra. Os resultados da anÃlise genÃtica utilizando os iniciadores Aero16S-F e Aero16S-R mostraram que todos os isolados identificados atravÃs de testes clÃssicos de microbiologia foram confirmados por meio da tÃcnica de PCR e 65,6% das espÃcies identificadas por testes bioquÃmicos convencionais foram confirmadas pelo mÃtodo de Box-PCR.
Books on the topic "Isolated influence of factors"
1
Educational Resources Information Center (U.S.), ed. Factors that influence teacher attrition. National Association of State Directors of Special Education, 1995.
Find full text
2
Mullen, Elizabeth Anne. An inhibitor of angiogenesis isolated from bovine cornea. s.n.], 1992.
Find full text
3
Harrison, M. Contingent factors in lateral influence attempts. Oxford Brookes University, 1995.
Find full text
4
LOK, Johnny Ch. Factors Influence Performance: Effectiveness. Independently Published, 2019.
Find full text
5
LOK, Johnny Ch. Factors Influence Organizational Development. Independently Published, 2020.
Find full text
6
LOK, Johnny Ch. Factors Influence Organization Performance. Independently Published, 2020.
Find full text
7
LOK, Johnny Ch. Factors Influence Performance Effectiveness. Independently Published, 2019.
Find full text
8
LOK, Johnny Ch. Factors Influence Performance Effectiveness. Independently Published, 2019.
Find full text
9
LOK, Johnny Ch. Factors Influence Performance Effectiveness. Independently Published, 2019.
Find full text
10
LOK, Johnny Ch. Factors Influence Efficient Productivity. Independently Published, 2019.
Find full textBook chapters on the topic "Isolated influence of factors"
1
Stieger, Pia A., and Urs Feller. "Influence of External Factors on the Catabolism of Stromal Proteins in Isolated Pea Chloroplasts." In Photosynthesis: from Light to Biosphere. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_968.
Full text
2
Broadbent, Ewen. "Factors of General Influence." In The Military and Government. Palgrave Macmillan UK, 1988. http://dx.doi.org/10.1007/978-1-349-09245-1_6.
Full text
3
Stronach, Sandra M., Thomasine Rudd, and John N. Lester. "Influence of Environmental Factors." In Biotechnology Monographs. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71215-9_4.
Full text
4
Ignatiev, Alexander A., and Alexander V. Lyashenko. "Influence of External Factors." In Heteromagnetic Microelectronics. Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-6002-3_9.
Full text
5
Farrell, Ann H., Katerina N. Schiralli, and Anthony A. Volk. "Factors That Influence Bullying." In Encyclopedia of Evolutionary Psychological Science. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-16999-6_162-1.
Full text
6
Deavours, Danielle. "Nonverbal neutrality influence factors." In Nonverbal Neutrality of Broadcasters Covering Crisis. Routledge, 2023. http://dx.doi.org/10.4324/9781003375340-4.
Full text
7
Farrell, Ann H., Katerina N. Schiralli, and Anthony A. Volk. "Factors That Influence Bullying." In Encyclopedia of Evolutionary Psychological Science. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-319-19650-3_162.
Full text
8
Burdon, R. H. "Isolated Cells as Experimental Models." In The Influence of New Technology on Medical Practice. Macmillan Education UK, 1988. http://dx.doi.org/10.1007/978-1-349-09609-1_20.
Full text
9
Schulz-Aellen, Marie-Françoise. "Factors That Influence Human Longevity." In Aging and Human Longevity. Birkhäuser Boston, 1997. http://dx.doi.org/10.1007/978-1-4612-2006-0_2.
Full text
10
Schulz-Aellen, Marie-Françoise. "Factors That Influence Human Longevity." In Aging and Human Longevity. Birkhäuser Boston, 1997. http://dx.doi.org/10.1007/978-1-4612-2448-8_3.
Full textConference papers on the topic "Isolated influence of factors"
1
Sreekumari, Kurissery R., Kanavillil Nandakumar, and Yasushi Kikuchi. "Effect of Metal Microstructure on Bacterial Attachment: a Contributing Factor for Preferential MIC Attack of Welds." In CORROSION 2004. NACE International, 2004. https://doi.org/10.5006/c2004-04597.
Full textAbstract:
Abstract Majority of microbiologically influenced corrosion failure cases is reported at or near welds. Experiments on bacterial attachment on AISI type 304 stainless steel welds and base metals were carried out to investigate whether factors other than surface roughness influence the attachment. Coupon exposure studies were done using two heterotrophic bacteria Pseudomonas sp. and Bacillus sp. isolated from MIC failure cases. Results showed that bacteria colonized more on weld metal coupons than on basemetal. Experiments that addressed the role of grain boundary/austenite-ferrite interfaces on bacterial attachment showed that initial attachment occurred along the grain boundary/austenite-ferrite interfaces, suggesting the influence of microstructure. Elemental segregation during welding and/or differential energy distribution between matrix and grain boundaries were suggested as the possible reasons for this pattern of attachment. Mitigation of bacterial attachment by altering the alloying element composition and microstructure is discussed.
2
Khuraibut, Yousef. "Failure of 24” Common Crude Piping and Formation of Iron Sulfides Due to the Influence of Sulfate & Iron Reducing Bacteria and Methanogens." In CONFERENCE 2022. AMPP, 2022. https://doi.org/10.5006/c2022-17545.
Full textAbstract:
Abstract A leak suddenly occurred at the 24-inch common crude piping from the separators heading to the degassing boot inlets and the wet crude tanks at an oil gathering center in Kuwait Oil Company. The initial observations showed the leak was due to a deep isolated pit and localized corrosion. Consequently, the piping spool was opened, deposits were collected for analysis and an action report was issued recommending replacing the leaky spool. Additional inspection by manual and long range ultrasonic thickness measurements for the 24” common crude line showed similar deep isolated pits (up to 70% thickness reduction) scattered across the length of the 1100 meter piping. This was extremely concerning since the facility has just been commissioned and been in service for 1.5 - 2 years only. Subsequently, an investigation was carried to determine the root causes for the failure concluded that an active Microbiologically Influenced Corrosion (MIC) possibly was taking place due to the influence of sulfate & iron reducing bacteria and methanogens. These different bacteria types formed biofilms at different pockets leading to deep isolated pitting, and the formation of iron sulfide layer found adhered at the 6 o'clock position along the length of the piping. The iron sulfide layer observed was confirmed to be present due to the activities of sulfate reducing bacteria and influenced by iron reducing bacteria and methanogens, since H2S was found in the crude stream at extremely low content in gas (1 ppm). Additionally, other factors such as flow velocity have contributed to the MIC and pitting / localized corrosion as it was found to be below 1 m/s, which promoted stagnation and stagnant conditions in the process stream. Further, calcium carbonates (CaCO3) played a major part in the observed damages, since it was formed non-uniformly across the length of the piping, promoting exposed pockets for biofilm formation, tubercles and under deposit corrosion. The results of this interesting investigation is presented in this paper.
3
Rodin, Vladimir B., Svetlana K. Zhigletsova, Nadezda A. Zhirkova, et al. "Altering Enviromental Composition as a Potential Method for Reversing Microbiologically Influenced Corrosion." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05498.
Full textAbstract:
Abstract The dynamics of corrosion losses caused by biofilms after changing culture conditions was investigated. Mild steel coupons were kept in the presence of biofilm generated by natural microbial micro-environment isolated from oil-processing waters. In addition to oil-oxidizing aerobes, the biofilm comprised sulphate-reducing bacteria. During formation of biofilm in glucose-mineral medium with peptone (GMP), corrosion losses increased vs control. The decrease of losses vs control was observed after transferring coupons carrying this biofilm into enriched with peptone Luria-Bertani medium (LB). During formation of biofilm in LB medium, corrosion losses decreased vs control. The increase of losses vs control was observed after transferring coupons with this biofilm into glucose-mineral medium. The high level of corrosion losses under intensive aeration conditions was registered. As soon as coupons with the biofilms were transferred into microaerophilic conditions, corrosion losses reduced sharply. Thus, it was confirmed that the subdivision of microorganisms into destructors and protectors is arbitrary. Results conclude that due to their combined metabolic activity, biofilms of natural microbial assemblages promptly respond to environmental factors, thereby either stimulating or inhibiting corrosion processes. This provides new opportunities for developing a promising approach to control MIC.
4
Richey, Edward, David S. Morton, and William C. Moshier. "Influence of Specimen Size on the SCC Growth Rate of Ni-Alloys Exposed to High Temperature Water." In CORROSION 2006. NACE International, 2006. https://doi.org/10.5006/c2006-06513.
Full textAbstract:
Abstract Tests were conducted on a single heat of Alloy 600 using compact tension specimens ranging from 50.80 mm (2 inches) in gross thickness (2T) to 10.16 mm (0.4 inches, 0.4T) in gross thickness. Results indicated that at stress intensity factor (K) levels above 55 MPa√m, the growth rate is affected by specimen size in deaerated primary water. The growth rate can be significantly faster in 0.4T and 0.6T (15.24 mm = 0.6 inches in gross thickness) specimens at these elevated K levels compared to 2T specimens. Stress corrosion crack (SCC) growth rates &gt; 6 x 10-7 mm/s were observed at 338°C and 40 cc/kg H2 in 0.6T and 0.4T specimens at these elevated K levels, although the fracture mode was not significantly affected by the specimen size. The SCC growth rate of 2T specimens under comparable test conditions was ~6 x 10-8 mm/s. All of the specimens examined that were tested at K &gt; 55 MPa√m exhibited intergranular failure, although ductile dimples and cracked grains were observed in the 0.4T specimens loaded to the elevated K levels. The effect of specimen size on the crack growth behavior indicated by electric potential drop (EPD) monitoring at K &gt; 55 MPa√m was also reviewed. EPD indicated steady state crack growth during the tests conducted on 1T (25.4 mm = 1.0 inches in gross thickness) and 2T specimens. Steady state crack growth was not indicated by EPD for the 0.4T and 0.6T specimens loaded at K &gt; 55 MPa√m. EPD indicated large jumps in the crack length at discrete points. Initially, it was believed that these large, rapid increases in the crack length corresponded to ductile tearing of uncracked ligaments in the crack wake as the SCC crack advanced. However, examination of the fracture surfaces did not reveal any evidence of isolated regions of ductile tearing in the crack wake. The large increases in the EPD signal were due to strain bursts. These results highlight the need to base SCC growth rates on destructive examination of the specimen.
5
Moore, Zachary, Lokesh Silwal, Aditya Vijayaraj, and Vrishank Raghav. "Quantification of the Aerodynamic Interference for Counter-Rotating Coaxial Rotors In-Ground Effect." In Vertical Flight Society 80th Annual Forum & Technology Display. The Vertical Flight Society, 2024. http://dx.doi.org/10.4050/f-0080-2024-0004.
Full textAbstract:
The performance of a coaxial rotor hovering in-ground effect (IGE) is compared against the out-of-ground effect (OGE) condition to quantify the rotor-ground interaction and against an isolated rotor IGE at equivalent blade loading to quantify the rotor-rotor interaction. It is observed that the performance of the coaxial rotor improves when it hovers IGE. However, the rotor-rotor and rotor-ground interactions compete, which affects the performance of the coaxial rotor. This paper aims to quantitatively measure the aerodynamic interactions of the CCR in IGE by developing a theoretical framework based on momentum theory. This formulation introduced induced power factors to understand the aerodynamic interactions of a CCR operating IGE. The performance measurements show that the rotor-ground interaction in the CCR system behaved similarly to a single rotor operating in IGE conditions. The interactional effects significantly influence the individual rotors as the rotor-rotor interactions get modified in the presence of ground. At the extreme ground height, the rotor-rotor interaction on the upper rotor decreased, while that for the lower rotor increased when the ground height was reduced. However, with an increase in the inter-rotor spacing, the performance of the lower rotor degrades while that of the upper rotor improves. At the (z/R)l = 0.4, the performance of the upper rotor in IGE improves by ∼ 8%, whereas the lower rotor performance degrades by 8% when compared to their respective OGE conditions.
6
RACU, Igor, and Florin-Iulian DRANGA. "The impact of the disorganized family on the personality formation of adolescents." In Ştiință și educație: noi abordări și perspective. "Ion Creanga" State Pedagogical University, 2023. http://dx.doi.org/10.46727/c.v1.24-25-03-2023.p67-71.
Full textAbstract:
The factors that influence children’s development are multiple and complex at the same time. Approaching a case of a child who comes from a disorganized family, we will not be able to say with certainty how the child would have developed without this factor, but there are a number of common characteristics that children who come from such families can develop. All these negative influences on the child’s development are mediated by the child’s protective factors, by their personal resources, through which they manage to cope with the maladaptive environment, to a greater or lesser extent. The child’s belonging to such a family can also influence the child’s personality. Children from dysfunctional families are also more likely to become withdrawn and socially isolated. They often feel lonely and have difficulty expressing their feelings and are at risk of developing depression, low self-esteem, anxiety and more. As children mature, these problems persist. Recent studies indicate that personality has a hereditary character in a percentage of 40% (Vukasović, 2015). Therefore, the major percentage is due to environmental factors. Thus, a new proposed direction is the research of the personality factors of children who come from disorganized families.
7
Shimmyo, Hiroshi, Tolga Onal, Shozo Nakamura, and Kazuya Tokunaga. "Influence of Mullins and hardening effects of seismic isolation rubber bearings on the seismic response." In IABSE Congress, Ghent 2021: Structural Engineering for Future Societal Needs. International Association for Bridge and Structural Engineering (IABSE), 2021. http://dx.doi.org/10.2749/ghent.2021.1444.
Full textAbstract:
<p>In the current Specifications for Highway Bridges of the Japan Road Association, a bilinear force- displacement relationship is recommended as the hysteresis loops of the seismic isolation rubber bearings for the dynamic structural analysis of seismically isolated bridges. However, it has been confirmed that the restoring force characteristics of the actual devices are different from the bilinear model due to the Mullins effect and the hardening phenomenon of the rubber under large shear strains. In this study, the effect of these two factors on the seismic response of a bridge is investigated through the dynamic analysis with the tri-linear double target model considering the factors. The parameters of the model are obtained from product test results of lead-plugged laminated rubber bearings and high-damping laminated rubber bearings.</p>
8
Shimmyo, Hiroshi, Tolga Onal, Shozo Nakamura, and Kazuya Tokunaga. "Influence of Mullins and hardening effects of seismic isolation rubber bearings on the seismic response." In IABSE Congress, Ghent 2021: Structural Engineering for Future Societal Needs. International Association for Bridge and Structural Engineering (IABSE), 2021. http://dx.doi.org/10.2749/ghent.2021.1444.
Full textAbstract:
<p>In the current Specifications for Highway Bridges of the Japan Road Association, a bilinear force- displacement relationship is recommended as the hysteresis loops of the seismic isolation rubber bearings for the dynamic structural analysis of seismically isolated bridges. However, it has been confirmed that the restoring force characteristics of the actual devices are different from the bilinear model due to the Mullins effect and the hardening phenomenon of the rubber under large shear strains. In this study, the effect of these two factors on the seismic response of a bridge is investigated through the dynamic analysis with the tri-linear double target model considering the factors. The parameters of the model are obtained from product test results of lead-plugged laminated rubber bearings and high-damping laminated rubber bearings.</p>
9
Liu, Mu-jun, Chao-ran Dou, Guo-ping Yu, Yu-xiu Yao, and Pei-pei Guo. "Influence of environmental factors on freeze-thaw stability of glycosylated soy protein isolate emulsion." In 2015 International Conference on Food Hygiene, Agriculture and Animal Science. WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789813100374_0010.
Full text
10
Tang, B., J. Shi, and X. Zhang. "THE INFLUENCE OF THE CONTENTS OF DYNAMIC WINDOW VIEW ON THE HEALING EFFECT OF PEOPLE IN ISOLATED, CONFINED AND EXTREME ENVIRONMENT." In CIE 2023 Conference. International Commission on Illumination, CIE, 2023. http://dx.doi.org/10.25039/x50.2023.pp026.
Full textAbstract:
People working in isolated, confined, and extreme environments, such as aerospace, underwater, underground, and polar environments, face physical and psychological challenges with the lack of relations with the outside world. As a suitable healing method, a window view system can provide contact with the outside world and improve physiology and psychological condition. This study aims at the healing effect of different contents of dynamic window view on people in ICE environment. Thirteen window view contents were analysed and evaluated to explore the factors influencing the healing effects. The conclusions from different perspectives of physiology, mood, perceptual recovery, and alertness were only partially consistent. There was no significant difference in labels on physiological indicators. The view contents with labels of aesthetics and rendering had better mood improvement and recovery effect on attention. These results can provide references for optimizing the window view for better healing in ICE environment.
Reports on the topic "Isolated influence of factors"
1
Mojidra, Rushil, and Keri Ryan. Influence of Vertical Ground Motion on Bridges Isolated with Spherical Sliding Bearings. Pacific Earthquake Engineering Research Center, University of California, Berkeley, CA, 2019. http://dx.doi.org/10.55461/rynq3624.
Full textAbstract:
The motivation for this project developed from testing of a full scale building isolated with triple friction pendulum bearings on the E-defense shake table in Japan. The test demonstrated experimentally that the vertical component of ground motion can amplify both the base shear and the story acceleration in the isolated building. Vertical shaking introduced high-frequency variation in the axial force of the bearings, and, consequently, a high-frequency component in the bearing lateral force, which excited higher structural modes in the building. Since vertical bridges are flexible in the vertical direction because of long spans, similar effects may be observed in bridges. The objectives of this study are to develop a physical understanding of the amplification of responses and develop a simplified method to predict amplification of base shear in three-dimensional (3D) shaking relative to two-dimensional (2D) shaking, for bridges isolated with spherical sliding bearings. A series of ground motions with a wide range of vertical shaking intensity were applied to 3D models of bridges isolated with triple pendulum bearings (TPBs), both excluding the vertical component (2D motion) and including the vertical component (3D motion). This enabled the comparison of the bridge response under 2D and 3D shaking such that the direct effect of vertical shaking could be investigated. The selected ground motions were fit to target spectra in the horizontal and vertical directions, and divided into three groups based on vertical peak ground acceleration (PGAV). Multi-span concrete box girder bridges were selected for this study, as they are a prominent bridge type in California, and are suitable for seismic isolation. Models were developed for a 3-span, 45-ft wide, multi-column Base Model bridge; various superstructure and isolation-system parameter variations were implemented to evaluate the effect of these variations on the amplification of base shear. Response histories were compared for a representative motion from each ground-motion group under 2D and 3D shaking. Modal and spectral analyses were conducted to understand dynamic properties and behavior of the bridge under vertical motion. Based on simplified theory, a method to estimate the amplification of base shear due to vertical shaking was developed. The accuracy of the simplified method was assessed through a base shear normalized error metric, and different amplification factors were considered. Response history analysis showed significant amplification of base shear under 3D motion implying that exclusion of vertical component could lead to under estimation of demand shear forces on bridge piers. Deck acceleration spectral response at different locations revealed that a transverse-vertical modal coupling response was present in the Base Model bridge, which led to amplification of deck accelerations in addition to base shear due to excitation of the superstructure transverse mode. The simplified method predicted that in addition to the peak vertical ground acceleration base shear amplification depended on the isolation-system period (radius of curvature) and friction coefficient. The error in the simplified method was approximately constant across the range of isolation-system parameters. Variations in the bridge superstructure or substructure modeling parameters had only a minor effect on the base shear since the deck acts as a single mass sliding on isolators; therefore, the simplified method can be applied to a range of bridge models. The simplified method includes an amplification factor that indirectly represents the dynamic amplification of vertical acceleration from the ground to the isolation system. An amplification factor of 1.0 was found to be sufficiently conservative to estimate the base shear due to 3D shaking. The lack of apparent dynamic amplification could mean that the peak vertical acceleration is out-of-phase with the base shear. The simplified method is more likely to be unconservative for high-intensity vertical ground motions due to the complexities associated with uplift and pounding. Further investigation is recommended to determine the threshold shaking intensity limit for the simplified method.
2
Emiliano, Diaz, and Jaspreet Singh. From linear insights to systemic solutions: the future of behavioral science. Busara, 2024. https://doi.org/10.62372/cesi7494.
Full textAbstract:
Behavioral Science has traditionally focused on understanding and influencing human behavior by identifying factors driving specific and directly related decisions. This linear approach, simplifies complex scenarios into isolated variables, and has provided the foundational insights for developing targeted interventions. While this perspective has proven effective in many cases, it may only sometimes fully capture the broader context in which behaviors occur, as a linear understanding alone is insufficient to grasp the complexities of human behavior fully. It misses important considerations like ripple effects and second-order effects. This is where systems thinking emerges as a valuable complement to applied behavioral science. By shifting from a singular, cause-and-effect perspective to a multi-layered, multidimensional approach, systems thinking allows us to see behavior not as an isolated event but as part of a broader system influenced by many interconnected factors that interact in dynamic and often unpredictable ways.
3
Musa, Padde, Zita Ekeocha, Stephen Robert Byrn, and Kari L. Clase. Knowledge Sharing in Organisations: Finding a Best-fit Model for a Regulatory Authority in East Africa. Purdue University, 2021. http://dx.doi.org/10.5703/1288284317432.
Full textAbstract:
Knowledge is an essential organisational asset that contributes to organisational effectiveness when carefully managed. Knowledge sharing (KS) is a vital component of knowledge management that allows individuals to engage in new knowledge creation. Until it’s shared, knowledge is considered useless since it resides within the human brain. Public organisations specifically, are more involved in providing and developing knowledge and hence can be classified as knowledge-intensive organisations. Scholarly research conducted on KS has proposed a number of models to help understand the KS process between individuals but none of these models is specifically for a public organisation. Moreover, to really reap the benefits that KS brings to an organization, it’s imperative to apply a model that is attributable to the unique characteristics of that organisation. This study reviews literature from electronic databases that discuss models of KS between individuals. Factors that influence KS under each model were isolated and the extent of each of their influence on KS in a public organization context, were critically analysed. The result of this analysis gave rise to factors that were thought to be most critical in understanding KS process in a public sector setting. These factors were then used to develop a KS model by categorizing them into themes including organisational culture, motivation to share and opportunity to share. From these themes, a KS model was developed and proposed for KS in a medicines regulatory authority in East Africa. The project recommends that an empirical study be conducted to validate the applicability of the proposed KS model at a medicines regulatory authority in East Africa.
4
Lu, Tianjun, Jian-yu Ke, Fynnwin Prager, and Jose N. Martinez. “TELE-commuting” During the COVID-19 Pandemic and Beyond: Unveiling State-wide Patterns and Trends of Telecommuting in Relation to Transportation, Employment, Land Use, and Emissions in Calif. Mineta Transportation Institute, 2022. http://dx.doi.org/10.31979/mti.2022.2147.
Full textAbstract:
Telecommuting, the practice of working remotely at home, increased significantly (25% to 35%) early in the COVID-19 pandemic. This shift represented a major societal change that reshaped the family, work, and social lives of many Californians. These changes also raise important questions about what factors influenced telecommuting before, during, and after COVID-19, and to what extent changes in telecommuting have influenced transportation patterns across commute modes, employment, land use, and environment. The research team conducted state-level telecommuting surveys using a crowd-sourced platform (i.e., Amazon Mechanical Turk) to obtain valid samples across California (n=1,985) and conducted state-level interviews among stakeholders (n=28) across ten major industries in California. The study leveraged secondary datasets and developed regression and time-series models. Our surveys found that, compared to pre-pandemic levels, more people had a dedicated workspace at home and had received adequate training and support for telecommuting, became more flexible to choose their own schedules, and had improved their working performance—but felt isolated and found it difficult to separate home and work life. Our interviews suggested that telecommuting policies were not commonly designed and implemented until COVID-19. Additionally, regression analyses showed that telecommuting practices have been influenced by COVID-19 related policies, public risk perception, home prices, broadband rates, and government employment. This study reveals advantages and disadvantages of telecommuting and unveils the complex relationships among the COVID-19 outbreak, transportation systems, employment, land use, and emissions as well as public risk perception and economic factors. The study informs statewide and regional policies to adapt to the new patterns of telecommuting.
5
Perry, Anna. Factors Influence Consumers' Purchase Intention of Smart Closets. Iowa State University, Digital Repository, 2016. http://dx.doi.org/10.31274/itaa_proceedings-180814-1461.
Full text
6
Morton, April M., Olufemi A. Omitaomu, Susan M. Kotikot, Elizabeth L. Held, and Budhendra L. Bhaduri. Evaluation of Factors that Influence Residential Solar Panel Installations. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1427606.
Full text
7
Varney, Shawn M., Lisa M. Mannina, Vikhyat S. Bebarta, Victoria J. Ganem, Katherine R. Carey, and Rosemarie Ramos. Factors that Influence Physician Identification of Potential Opioid Misusers. Defense Technical Information Center, 2013. http://dx.doi.org/10.21236/ada586766.
Full text
8
Grumet, Rebecca, and Benjamin Raccah. Identification of Potyviral Domains Controlling Systemic Infection, Host Range and Aphid Transmission. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7695842.bard.
Full textAbstract:
Potyviruses form one of the largest and most economically important groups of plant viruses. Individual potyviruses and their isolates vary in symptom expression, host range, and ability to overcome host resistance genes. Understanding factors influencing these biological characteristics is of agricultural importance for epidemiology and deployment of resistance strategies. Cucurbit crops are subject to severe losses by several potyviruses including the highly aggressive and variable zucchini yellow mosaic virus (ZYMV). In this project we sought to investigate protein domains in ZYMV that influence systemic infection and host range. Particular emphasis was on coat protein (CP), because of known functions in both cell to cell and long distance movement, and helper component-protease (HC-Pro), which has been implicated to play a role in symptom development and long distance movement. These two genes are also essential for aphid mediated transmission, and domains that influence disease development may also influence transmissibility. The objectives of the approved BARD project were to test roles of specific domains in the CP and HC-Pro by making sequence alterations or switches between different isolates and viruses, and testing for infectivity, host range, and aphid transmissibility. These objectives were largely achieved as described below. Finally, we also initiated new research to identify host factors interacting with potyviral proteins and demonstrated interaction between the ZYMV RNA dependent RNA polymerase and host poly-(A)-binding protein (Wang et al., in press). The focus of the CP studies (MSU) was to investigate the role of the highly variable amino terminus (NT) in host range determination and systemic infection. Hybrid ZYMV infectious clones were produced by substituting the CP-NT of ZYMV with either the CP-NT from watermelon mosaic virus (overlapping, but broader host range) or tobacco etch virus (TEV) (non- overlapping host range) (Grumet et al., 2000; Ullah ct al., in prep). Although both hybrid viruses initially established systemic infection, indicating that even the non-cucurbit adapted TEV CP-NT could facilitate long distance transport in cucurbits, after approximately 4-6, the plants inoculated with the TEV-CPNT hybrid exhibited a distinct recovery of reduced symptoms, virus titer, and virus specific protection against secondary infection. These results suggest that the plant recognizes the presence of the TEV CP-NT, which has not been adapted to infection of cucurbits, and initiates defense responses. The CP-NT also appears to play a role in naturally occurring resistance conferred by the zym locus in the cucumber line 'Dina-1'. Patterns of virus accumulation indicated that expression of resistance is developmentally controlled and is due to a block in virus movement. Switches between the core and NT domains of ZYMV-NAA (does not cause veinal chlorosis on 'Dina-1'), and ZYMV-Ct (causes veinal chlorosis), indicated that the resistance response likely involves interaction with the CP-NT (Ullah and Grumet, submitted). At the Volcani Center the main thrust was to identify domains in the HC-Pro that affect symptom expression or aphid transmissibility. From the data reported in the first and second year report and in the attached publications (Peng et al. 1998; Kadouri et al. 1998; Raccah et al. 2000: it was shown that: 1. The mutation from PTK to PAK resulted in milder symptoms of the virus on squash, 2. Two mutations, PAK and ATK, resulted in total loss of helper activity, 3. It was established for the first time that the PTK domain is involved in binding of the HC-Pro to the potyvirus particle, and 4. Some of these experiments required greater amount of HC-Pro, therefore a simpler and more efficient purification method was developed based on Ni2+ resin.
9
Yoo, Jeong-Ju, and Seahee Lee. Identifying Factors Influence Individuals' Intention to Quit Tanning: Sociocultural Perspective. Iowa State University, Digital Repository, 2017. http://dx.doi.org/10.31274/itaa_proceedings-180814-1823.
Full text
10
Dyer, J., and J. Shipmon. Analysis of Factors that Influence Infiltration Rates using the HELP Model. Office of Scientific and Technical Information (OSTI), 2017. http://dx.doi.org/10.2172/1395976.
Full text