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Journal articles on the topic 'Isolation techniques'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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1

Brodsky, Jay B., and Sean Mackey. "Isolation Techniques." Seminars in Cardiothoracic and Vascular Anesthesia 1, no. 3 (September 1997): 225–35. http://dx.doi.org/10.1177/108925329700100305.

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2

Campos, Javier H. "LUNG ISOLATION TECHNIQUES." Anesthesiology Clinics of North America 19, no. 3 (September 2001): 455–74. http://dx.doi.org/10.1016/s0889-8537(05)70243-1.

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3

Li, Pin, Melisa Kaslan, Sze Han Lee, Justin Yao, and Zhiqiang Gao. "Progress in Exosome Isolation Techniques." Theranostics 7, no. 3 (2017): 789–804. http://dx.doi.org/10.7150/thno.18133.

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4

Sinanoglu, O., and T. Petrov. "Isolation Techniques for Soft Cores." IEEE Transactions on Computer-Aided Design of Integrated Circuits and Systems 27, no. 8 (August 2008): 1453–66. http://dx.doi.org/10.1109/tcad.2008.925794.

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5

Mattar, Rahif E., Ayman M. Sulimany, Saad S. Binsaleh, and Ibrahim M. Al-Majed. "Comparison of Fissure Sealant Chair Time and Patients’ Preference Using Three Different Isolation Techniques." Children 8, no. 6 (May 25, 2021): 444. http://dx.doi.org/10.3390/children8060444.

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This randomized clinical trial aimed to evaluate the patient’s preference and chair time needed during pit and fissure sealant placement under three isolation techniques (Isolite system, rubber dam isolation, and cotton roll isolation). Participants, aged 6–15 years and requiring four sealants on the first or second permanent molars, attending the pediatric dental clinics at King Saud University in Saudi Arabia were enrolled according to the inclusion criteria. Each participant received sealants on three random first or second permanent molars using three isolation techniques. The time required for sealant placement was recorded for each technique. Following sealant placement, an interview-based questionnaire was administered to the participants to evaluate their preference regarding the isolation techniques. Forty-eight children (23 male and 25 female) with a mean age of 8.58 ± 1.93 years participated in this study. The mean chair times were 248.14, 255.89, and 243.29 s for the Isolite system, rubber dam isolation, and cotton roll isolation, respectively. Approximately 79% of participants considered cotton roll isolation to be the most comfortable, whereas approximately 71% were significantly less likely to use rubber dam isolation again. In conclusion, there were no significant differences in sealant placement time among the three isolation techniques. However, cotton roll isolation was the technique that was most preferred by the participants.
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6

GREGORY, SEAN M., and JAMES S. QUINN. "Microsatellite isolation from four avian species comparing two isolation techniques." Molecular Ecology Notes 6, no. 1 (March 2006): 87–89. http://dx.doi.org/10.1111/j.1471-8286.2005.01148.x.

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7

Chen, Shaojie, Boris Schmidt, Stefano Bordignon, Fabrizio Bologna, Takahiko Nagase, Laura Perrotta, and K. R. Julian Chun. "Practical Techniques in Cryoballoon Ablation: How to Isolate Inferior Pulmonary Veins." Arrhythmia & Electrophysiology Review 7, no. 1 (2018): 11. http://dx.doi.org/10.15420/aer.2018;1;2.

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Catheter ablation is the most effective treatment option for patients suffering from symptomatic atrial fibrillation. Electrical isolation of the pulmonary veins is the procedural cornerstone. Point-by-point radiofrequency current energy ablation in combination with a 3D electro-anatomical mapping system is the established approach to ablation. In contrast, cryoballoon ablation uses a single-shot approach to facilitate pulmonary vein isolation. However, fixed cryoballoon diameters (28 mm or 23 mm) and non-balloon compliance can lead to technical difficulties in isolating variable pulmonary vein anatomies. This review focuses on key procedural aspects and illustrates practical techniques in cryoballoon pulmonary vein isolation to shorten the learning curve without compromising safety and efficacy. It has a special emphasis on inferior pulmonary veins.
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8

Poyai, A., E. Simoen, C. Claeys, R. Rooyackers, and G. Badenes. "Lifetime study in advanced isolation techniques." Materials Science in Semiconductor Processing 4, no. 1-3 (February 2001): 137–39. http://dx.doi.org/10.1016/s1369-8001(00)00165-7.

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9

Nyangoni, K., S. Balachandran, and I. Hodzovic. "National survey on lung isolation techniques." European Journal of Anaesthesiology 27 (June 2010): 249. http://dx.doi.org/10.1097/00003643-201006121-00803.

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10

Shu, Rui, Peipei Wang, Sigmund A. Gorski III, Benjamin Andow, Adwait Nadkarni, Luke Deshotels, Jason Gionta, William Enck, and Xiaohui Gu. "A Study of Security Isolation Techniques." ACM Computing Surveys 49, no. 3 (December 13, 2016): 1–37. http://dx.doi.org/10.1145/2988545.

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11

Park, S.-T., and T.-T. Luu. "Techniques for optimizing parameters of negative stiffness." Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science 221, no. 5 (May 1, 2007): 505–10. http://dx.doi.org/10.1243/0954406jmes390.

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Passive isolation systems based on springs are often used to resist vibration due to their low cost and reliability. The basic principle of these systems is that they try to decrease the natural frequency as low as possible. Stiffness of the overall spring system must be low to make the isolator achieve the low natural frequency. An approach to obtain the low dynamic stiffness is the combination of a negative stiffness system and a common isolator. There are, however, a lot of negative stiffness systems, and selecting the best one is difficult. In this paper, mathematical analyses are used to determine the best system that is then applied to construct a vertical isolator of the anti-vibration table.
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12

Guo, Linchuan, Xu Wang, Rang-Lin Fan, and Fengrong Bi. "Review on Development of High-Static–Low-Dynamic-Stiffness Seat Cushion Mattress for Vibration Control of Seating Suspension System." Applied Sciences 10, no. 8 (April 22, 2020): 2887. http://dx.doi.org/10.3390/app10082887.

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This review focuses on studying passive vibration isolation techniques and their applications. Advantages and disadvantages of different vibration isolation techniques will be compared in order to find an innovative method to reduce seating suspension system vibration and improve the ride comfort of vehicles’ drivers. Quasi-zero stiffness (QZS) isolators of high-static–low-dynamic stiffness are found to have good application prospects in low-frequency vibration isolation. In order to improve the isolation performance of the seating suspension system, a specially made seat cushion will be studied where a quasi-zero stiffness (QZS) isolator such as a double-diamond isolator is selected as one of the cells/units/elements of the seat cushion mattress. The double-diamond isolator structure does not represent the whole seating suspension system. The transmissibility ratio of the double-diamond isolator is evaluated by analytical and simulation models and compared to that of a conventional linear spring isolator. Although the performance of the passive vibration isolation/control system is usually worse than that of the active or semi-active control system, the cost of the passive vibration isolation/control system is lower than that of the active and semi-active control system, and the relative simplicity of adding a vibration isolation cushion mattress in an existing passive seating suspension system makes it low in cost, easy to implement, and more attractive than the active and semi-active vibration control systems.
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13

Ali D. Abbas, Ali D. Abbas. "Nurses’ Practices Concerning Isolation Techniques for Patients with Burns At Baghdad Teaching Hospitals." Paripex - Indian Journal Of Research 3, no. 8 (January 15, 2012): 140–47. http://dx.doi.org/10.15373/22501991/august2014/42.

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14

Desai, Sonal, and Pratima Tatke. "Phytochemical Markers: Classification, Applications and Isolation." Current Pharmaceutical Design 25, no. 22 (September 27, 2019): 2491–98. http://dx.doi.org/10.2174/1381612825666190709203239.

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Background: There has been aroused demand for herbal drugs/products worldwide because of their fewer side effects as compared to synthetic drugs. The major obstacle in the global acceptance of herbal products is the lack of proper standardization technique. Methods: Various test procedures have been used for authentication and quality control of botanicals among which marker based standardization has attained more attention. The major challenge faced by phytochemist is to select appropriate phytochemical marker for quality control of herbal drugs. Phytochemical markers used for standardization must be of known purity. Phytochemical markers which are not commercially available have to be isolated from respective medicinal plants. Various chromatographic techniques are reported for the purification of phytomarkers from plants. A comprehensive report on different purification techniques of isolation of phytochemical markers through in-depth review of scientific literature is required. Conclusion: This article highlights various classifications of phytochemical markers along with their applications in standardization of herbal drugs and various classical and modern analytical techniques for their isolation.
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15

Campos, Javier H., and Mark N. Gomez. "Current concepts in adult lung isolation techniques." Seminars in Anesthesia, Perioperative Medicine and Pain 21, no. 3 (September 2002): 182–95. http://dx.doi.org/10.1053/sane.2002.34187.

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16

Diez-Jimenez, Efren, Toshihiko Sugiura, Rocco Rizzo, Maria-Jesus Gómez-García, and Andrew Fleming. "Electromagnetic Techniques for Vibration Damping and Isolation." Shock and Vibration 2019 (November 26, 2019): 1–2. http://dx.doi.org/10.1155/2019/9139067.

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17

Widholm, Jack M. "Mutant isolation techniques with plant tissue cultures." Journal of Tissue Culture Methods 12, no. 4 (December 1989): 151–56. http://dx.doi.org/10.1007/bf01404442.

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18

Kuroki, Tamotsu, and Susumu Eguchi. "No-touch isolation techniques for pancreatic cancer." Surgery Today 47, no. 1 (March 1, 2016): 8–13. http://dx.doi.org/10.1007/s00595-016-1317-5.

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19

Moťková, P., and J. Vytřasová. "Comparison of methods for isolating fungal DNA." Czech Journal of Food Sciences 29, Special Issue (January 4, 2012): S76—S85. http://dx.doi.org/10.17221/266/2011-cjfs.

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In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification before the proper DNA detection itself. For this purpose, classic methods of DNA extraction were compared and optimised while isolations using commercial kits were also done. The methods were evaluated from several perspectives, with focus especially laid on the isolated DNA not contain PCR inhibitors which would prevent DNA amplification, thus inhibiting the detection itself. For optimising the individual methods, collection strains of the genus Aspergillus were used. After the evaluation, two most suitable methods were selected and chosen for isolating potentially aflatoxigenic moulds taken from food samples. These methods were the commercially supplied kit for isolating DNA from plant leaves from Sigma and a classic method according Cenis in combination with the cell wall disruption by means of liquid nitrogen.
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20

S., Satheesh Kumar. "Fault Detection Techniques in Solar Photovoltaic Modules by Using KNN and Isolation Forest Algorithms." Journal of Advanced Research in Dynamical and Control Systems 12, SP4 (March 31, 2020): 153–62. http://dx.doi.org/10.5373/jardcs/v12sp4/20201477.

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21

Belajová, E., and D. Rauová. "Comparison of two clean up techniques in isolation of ochratoxin A from red wine." Czech Journal of Food Sciences 28, No. 3 (July 1, 2010): 233–41. http://dx.doi.org/10.17221/101/2008-cjfs.

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Two procedures for the extraction of ochratoxin A (OTA) from red wine &ndash; the reference clean up procedure using specific immunoaffinity column (IAC), and solid phase extraction (SPE) in which an active carbon was employed, was compared. In SPE procedure, various mixtures of dichloromethane (D), toluene (T), acetonitrile (AC), methanol (M), and acetic acid (A) were used as OTA desorption agents. Two types of SPE carbonaceous columns were tested &ndash; commercial SPE columns (Supelclean<sup>TM</sup> Envi-Carb) with a nonporous graphitised carbon, and SPE columns prepared in our laboratory (further specified as Lab-Carb) that were filled with a micro particular granular carbon. OTA was extracted from spiked red wine by the use of both carbonaceous columns. The highest OTA mean recovery calculated in relation to the reference IAC procedure was 98.5%, using the Lab-Carb adsorbent and acetonitrile + toluene, 3 + 1 (v + v) as the elution mixture (OTA spike levels of 0.2 &micro;g/l). Using the elution mixture of dichloromethane + methanol, 9 + 1 (v + v), the relative recoveries of 76.4% and 82.9% were reached at the OTA spike levels of 0.2 &micro;g/l and 1.9 &micro;g/l, respectively. The application of Envi-Carb adsorbent generally resulted in a very poor OTA recovery under the experimental conditions used (less than 50%). OTA was detected by liquid chromatography with fluorescence detection (LC-FLD) providing the detection limit of 0.011 &micro;g/l and the quantification limit of 0.033 &micro;g/l. &nbsp;
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22

Mangalgi, Smita, and Annapurna Sajjan. "Comparison of Three Blood Culture Techniques in the Diagnosis of Human Brucellosis." Journal of Laboratory Physicians 6, no. 01 (January 2014): 014–17. http://dx.doi.org/10.4103/0974-2727.129084.

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ABSTRACT Background: Isolation of Brucellai is the gold standard in the laboratory diagnosis of brucellosis. As Brucellai is intracellular and the number of circulating bacteria is usually low, removal/dilution of antibacterial substances, concentration of bacteria and optimal culture conditions may enhance the rate of isolation. Aims and Objectives: The objective of the following study was to compare the lysis concentration (LC), clot culture and conventional Castaneda blood culture techniques for the isolation rate and recovery time in the diagnosis of human brucellosis. Materials and Methods: Blood cultures by LC, clot culture and conventional method were performed in 169 patients who had antibody titers ≥160 international units by the serum agglutination test. Results: Overall blood culture positivity was found to be 24.8%, 43.1% and 34.9% by conventional, LC and clot culture techniques in that order. The mean recovery time by LC and clot culture techniques was significantly less than conventional method, resulting in an overall difference of nearly 6 and 4 days respectively. Conclusions: For the isolation of Brucellai from blood, LC and clot culture techniques are better than the conventional technique.
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23

Kao-Kniffin, Jenny, Sarah M. Carver, and Antonio DiTommaso. "Advancing Weed Management Strategies Using Metagenomic Techniques." Weed Science 61, no. 2 (June 2013): 171–84. http://dx.doi.org/10.1614/ws-d-12-00114.1.

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Global occurrences of herbicide resistant weed populations have increased the demand for development of new herbicides targeting novel mechanisms of action. Metagenomic approaches to natural drug discovery offer potential for isolating weed suppressive compounds from microorganisms. In past research, traditional techniques entailed isolating compounds from living organisms, whereas metagenomic approaches involve extracting fragments of DNA from soil and exploring for compounds of interest produced by the transformed hosts. Several herbicidal compounds have been isolated from soil bacteria through culturing methods and have led to the development of popular herbicides, such as glufosinate. In this review, we discuss the emergence of metagenomic approaches for weed management in the context of natural product discovery using traditional culture-dependent isolation and the more recent culture-independent methods. The same techniques can be used to isolate herbicide resistance genes. Adoption of metagenomic approaches in pest management research can lead to novel control strategies in cropping and landscape systems.
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24

Pérez-Aracil, J., E. Pereira, Iván M. Díaz, and P. Reynolds. "Passive and active vibration isolation under isolator-structure interaction: application to vertical excitations." Meccanica 56, no. 8 (March 25, 2021): 1921–35. http://dx.doi.org/10.1007/s11012-021-01342-2.

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AbstractThis work studies the influence of a vibration isolator on the response of a flexible base structure. Two strategies are compared: passive and active vibration isolation (PVI, AVI). Although the multiple advantages of AVI over PVI techniques are well known, their effect in the base structure has not to date been compared. This interaction has an important role in the performance of the general control system, especially when the vibration isolation system is not the only system on the base structure or when there are multiple isolators working simultaneously on it. In addition, the structural serviceability of the base structure can also be affected. The analysis of the vibration isolation problem is made from a wide perspective, including the effect that isolator has on the base structure. Hence assuming the base structure is a non-rigid system. The effect of the isolation system on the base response is studied for an extensive range of base structures, thus showing different possible scenarios. The influence is quantified by comparing the peak magnitude response of the base when both passive and active vibration isolation techniques are used. The theoretical results have been corroborated by undertaking experimental tests on a full-scale laboratory structure.
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25

Luu, T. T., and S. T. Park. "Techniques for increasing the effectiveness of anti-vibration in Scott-Russel linkage isolators." Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science 220, no. 12 (December 1, 2006): 1843–45. http://dx.doi.org/10.1243/0954406jmes383ft.

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The Scott-Russel horizontal isolator is a straight line linkage. When this structure is used, the stored energy can be reduced as much as possible, making the isolation system achieve a very low natural frequency. However, because of the existence of the weight of the isolator, the natural frequency is not as low as expected. In this paper, the equation of motion of the Scott-Russel isolator is proposed to find a method to reduce the natural frequency by choosing reasonable parameters and using the zero-length spring.
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26

Aybeke, Mehmet. "Simple Embryo Isolation Techniques from Ophrys (Orchidaceae) Seeds." Greener Journal of Agricultural Sciences 3, no. 6 (June 20, 2013): 448–50. http://dx.doi.org/10.15580/gjas.2013.3.030413509.

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27

Riley, Michael P. "Techniques to improve and confirm pulmonary vein isolation." Future Cardiology 11, no. 6 (November 2015): 655–62. http://dx.doi.org/10.2217/fca.15.63.

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28

Fröhlich, J. "New techniques for isolation of single prokaryotic cells." FEMS Microbiology Reviews 24, no. 5 (December 2000): 567–72. http://dx.doi.org/10.1016/s0168-6445(00)00045-0.

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29

Manafi, Sara, Muhannad A. Al-Tarifi, and Dejan S. Filipovic. "Isolation Improvement Techniques for Wideband Millimeter-Wave Repeaters." IEEE Antennas and Wireless Propagation Letters 17, no. 2 (February 2018): 355–58. http://dx.doi.org/10.1109/lawp.2018.2790398.

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30

Fröhlich, Jürgen, and Helmut König. "New techniques for isolation of single prokaryotic cells." FEMS Microbiology Reviews 24, no. 5 (December 2000): 567–72. http://dx.doi.org/10.1111/j.1574-6976.2000.tb00558.x.

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31

Weiskopf, Richard B., and Javier H. Campos. "Current Techniques for Perioperative Lung Isolation in Adults." Anesthesiology 97, no. 5 (November 1, 2002): 1295–301. http://dx.doi.org/10.1097/00000542-200211000-00036.

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32

Ross, Cliff, Steve Hevener, Roger Clark, James X. Hartmann, and Frank Mari. "Isolation of Parvalbumin Isotypes by Preparative HPLC Techniques." Preparative Biochemistry and Biotechnology 28, no. 1 (February 1998): 49–60. http://dx.doi.org/10.1080/10826069808010126.

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33

Hadnadjev, Miroslav, Tamara Dapcevic-Hadnadjev, Milica Pojic, Bojana Saric, Aleksandra Misan, Pavle Jovanov, and Marijana Sakac. "Progress in vegetable proteins isolation techniques: A review." Food and Feed Research 44, no. 1 (2017): 11–21. http://dx.doi.org/10.5937/ffr1701011h.

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34

Allwood, R. L. "Recent Developments in Subsea Pigging and Isolation Techniques." Underwater Technology 21, no. 3 (December 1, 1995): 25–26. http://dx.doi.org/10.3723/175605495783326360.

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35

Humphrey, T. J., and A. Whitehead. "Techniques for the isolation of salmonellas from eggs." British Poultry Science 33, no. 4 (September 1992): 761–68. http://dx.doi.org/10.1080/00071669208417517.

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36

Campos, Javier H. "Lung isolation techniques for patients with difficult airway." Current Opinion in Anaesthesiology 23, no. 1 (February 2010): 12–17. http://dx.doi.org/10.1097/aco.0b013e328331e8a7.

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37

Liangsupree, Thanaporn, Evgen Multia, and Marja-Liisa Riekkola. "Modern isolation and separation techniques for extracellular vesicles." Journal of Chromatography A 1636 (January 2021): 461773. http://dx.doi.org/10.1016/j.chroma.2020.461773.

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38

Mahgoub, Elham O., Ehsan Razmara, Amirreza Bitaraf, Fahimeh-Sadat Norouzi, Maryam Montazeri, Roudabeh Behzadi-Andouhjerdi, Mojtaba Falahati, et al. "Advances of exosome isolation techniques in lung cancer." Molecular Biology Reports 47, no. 9 (August 12, 2020): 7229–51. http://dx.doi.org/10.1007/s11033-020-05715-w.

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39

Royland, J. E., R. C. Wiggins, and G. W. Konat. "Myelin isolation: Comparison of sedimentation and flotation techniques." Neurochemistry International 21, no. 2 (September 1992): 171–75. http://dx.doi.org/10.1016/0197-0186(92)90143-f.

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40

DUMAS, JEAN-CHARLES, LI JU, PABLO BARRIGA, CHUNNONG ZHAO, ANDREW A. WOOLLEY, and DAVID G. BLAIR. "NOISE PERFORMANCE OF A 72 m SUSPENDED FABRY–PÉROT CAVITY." International Journal of Modern Physics D 20, no. 10 (September 2011): 2063–67. http://dx.doi.org/10.1142/s0218271811020184.

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We report on a seismic isolator with a relatively compact 3 m stack, combining new passive isolation techniques. It consists of three cascaded passive 3D isolator stages suspended from an Ultra Low Frequency (ULF) horizontal Robert linkage stage which itself is suspended from a ULF 3D pre-isolator. The 3D isolators use self-damping pendulums and Euler springs for the horizontal and vertical stages respectively, while the 3D pre-isolator is the combination of an inverse pendulum which provides low frequency horizontal pre-isolation, and a LaCoste linkage for low frequency vertical pre-isolation. Two isolators suspending mirror test masses have been built to form a 72 m optical cavity in order to test their performance. We report results which demonstrate residual motion at nanometer level at frequencies above 1 Hz.
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41

Sláma, P., Z. Sládek, and D. Ryšánek. "Effect of Isolation Techniques on Viability of Bovine Blood Neutrophils." Acta Veterinaria Brno 75, no. 3 (2006): 343–53. http://dx.doi.org/10.2754/avb200675030343.

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The effect of selected isolation methods on the viability of neutrophil granulocytes (neutrophils) from the blood of healthy Holstein x Bohemian Red Pied crossbred heifers was evaluated. Two methods of neutrophil isolation were used: a) neutrophil isolation on the basis of hypotonic erythrocyte lysis (in two variants: after the erythrocyte lysis proper, the cells were centrifuged at either 200 g or 1000 g), and b) neutrophil isolation with FACS Lysing Solution as the lysing agent. The viability of the isolated neutrophils was evaluated on the basis of apoptosis and necrosis. The results obtained with flow cytometry (FCM) suggest that, from the isolation techniques used, the method based on FACS Lysing Solution impaired the neutrophil viability least. After the application of this method, 5.36 ± 2.15% of neutrophils were apoptotic and 0.51 ± 0.12% were necrotic. In contrast, when the hypotonic erythrocyte lysis was used, the proportion of apoptotic neutrophils amounted to 42.14 ± 7.12% and 49.00 ± 14.70%, respectively, and 41.12 ± 5.55% and 36.91 ± 24.38% respectively of necrotic neutrophils (P < 0.01). This was also confirmed by the light microscopy. After the isolation with FASC Lysing Solution, 1.92 ± 1.74% of neutrophils were apoptotic and 1.05 ± 0.76% were necrotic, as distinct from after the hypotonic erythrocyte lysis where 9.43 ± 3.69% of neutrophils were apoptotic and 12.67 ± 4.74% of necrotic after centrifugation at 200 g, while 12.60 ± 4.35 were apoptotic and 14.96 ± 12.64% were necrotic after centrifugation at 1000 g. It follows from the above-mentioned data that hypotonic lysis is not a suitable method for the isolation of neutrophils, as the method itself markedly affects cell viability.
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42

Chongtham, Monika Chanu, Tamer Butto, Kanak Mungikar, Susanne Gerber, and Jennifer Winter. "INTACT vs. FANS for Cell-Type-Specific Nuclei Sorting: A Comprehensive Qualitative and Quantitative Comparison." International Journal of Molecular Sciences 22, no. 10 (May 19, 2021): 5335. http://dx.doi.org/10.3390/ijms22105335.

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Increasing numbers of studies seek to characterize the different cellular sub-populations present in mammalian tissues. The techniques “Isolation of Nuclei Tagged in Specific Cell Types” (INTACT) or “Fluorescence-Activated Nuclei Sorting” (FANS) are frequently used for isolating nuclei of specific cellular subtypes. These nuclei are then used for molecular characterization of the cellular sub-populations. Despite the increasing popularity of both techniques, little is known about their isolation efficiency, advantages, and disadvantages or downstream molecular effects. In our study, we compared the physical and molecular attributes of sfGFP+ nuclei isolated by the two methods—INTACT and FANS—from the neocortices of Arc-CreERT2 × CAG-Sun1/sfGFP animals. We identified differences in efficiency of sfGFP+ nuclei isolation, nuclear size as well as transcriptional (RNA-seq) and chromatin accessibility (ATAC-seq) states. Therefore, our study presents a comprehensive comparison between the two widely used nuclei sorting techniques, identifying the advantages and disadvantages for both INTACT and FANS. Our conclusions are summarized in a table to guide researchers in selecting the most suitable methodology for their individual experimental design.
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43

Omprakash Reddy, N., and A. Manchalwar. "Performance of 2-D Frame Equipped With Base isolation System under Dynamic Loadings." E3S Web of Conferences 184 (2020): 01099. http://dx.doi.org/10.1051/e3sconf/202018401099.

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Building responses have become a major concern in design research. Passive control techniques are implemented to improve structure efficiency. The present research aims to assess the efficiency of base isolation system for a 2D frame and to enhance the performance of structures that are subjected to seismic ground excitations and ground vibrations induced by blast. Two moments of resistant RC frames were studied and output of the isolator (Lead / Rubber Bearing) was observed to reduce structural responses. In SAP2000 non-linear dynamic analysis is conducted to compare normal and irregular moment-resistant frames and structural responses with and without passive control techniques. Isolators are constructed based on time of isolation. Reduction of structural responses is assessed by passive control techniques, and comparative analysis is performed. Mitigating systemic retaliation is affected by the implementation of passive control system.
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Perez, C. R. "Alternative Technique for Class V Resin Composite Restorations with Minimum Finishing/Polishing Procedures." Operative Dentistry 35, no. 3 (May 1, 2010): 375–79. http://dx.doi.org/10.2341/09-310-tr.

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Clinical Relevance Class V restorations are a common challenge to clinicians. These restorations involve problems, including isolation, adhesion, insertion technique, finishing and polishing. In order to solve these problems, several techniques have been developed. This article describes a new technique for the restoration of Class V cavities, one that eliminates rubber dam isolation and avoids any contamination. If used properly, this technique also provides good cervical contour, reducing or eliminating finishing and polishing procedures.
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Tubaldi, E., A. Dall’Asta, and L. Dezi. "Seismic Response Analysis of Continuous Multispan Bridges with Partial Isolation." Shock and Vibration 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/183756.

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Partially isolated bridges are a particular class of bridges in which isolation bearings are placed only between the piers top and the deck whereas seismic stoppers restrain the transverse motion of the deck at the abutments. This paper proposes an analytical formulation for the seismic analysis of these bridges, modelled as beams with intermediate viscoelastic restraints whose properties describe the pier-isolator behaviour. Different techniques are developed for solving the seismic problem. The first technique employs the complex mode superposition method and provides an exact benchmark solution to the problem at hand. The two other simplified techniques are based on an approximation of the displacement field and are useful for preliminary assessment and design purposes. A realistic bridge is considered as case study and its seismic response under a set of ground motion records is analyzed. First, the complex mode superposition method is applied to study the characteristic features of the dynamic and seismic response of the system. A parametric analysis is carried out to evaluate the influence of support stiffness and damping on the seismic performance. Then, a comparison is made between the exact solution and the approximate solutions in order to evaluate the accuracy and suitability of the simplified analysis techniques for evaluating the seismic response of partially isolated bridges.
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46

Dickman, Jameson. "A review of techniques and errors involved in sound isolation testing." INTER-NOISE and NOISE-CON Congress and Conference Proceedings 263, no. 3 (August 1, 2021): 3176–86. http://dx.doi.org/10.3397/in-2021-2322.

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Construction industry design standards are increasingly calling for new construction to be inspected and tested for compliance with design specifications after the project is built; otherwise known as the commissioning process. As part of this trend, owners, sensitive to the acoustics of their facilities, are seeking confirmation via measurements that their buildings meet sound isolation and background noise requirements, particularly when pursuing certifications under the US Green Building Council LEED standard, the WELL Building Standard, or other green building or wellness standards. In general, the error of sound isolation measurements is not officially established. This poses challenges to designers tasked with specifying assemblies and components to meet field verification requirements. This paper will briefly review current research and standards on the error of measurements such as Noise Isolation Class (NIC) and the Weighted Level Difference (D) and discuss example design standards and guidelines which do or do not account for this error. It will also propose further research topics to better define the error in sound isolation measurements and best practices when establishing or designing to sound isolation criteria in new or renovated buildings.
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Gnanaharan, I., and R. Anbazhagan. "Review on the Design of the Isolation Techniques for UWB-MIMO Antennas." Advanced Electromagnetics 7, no. 4 (August 31, 2018): 46–70. http://dx.doi.org/10.7716/aem.v7i4.743.

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Ultra wide band - Multiple Input Multiple Output antenna technology provides higher data rates and the combination of the ultra wide band (UWB) and the multiple input multiple output (MIMO) technologies provides a solution for the demand of still higher data rates i.e. in excess of 3 Gb/sec in the future. As the antenna technologies are improving, the size of the MIMO antenna is growing smaller and smaller. Placing the antenna elements in such close proximity increases the coupling between them. Various isolation techniques have to be introduced between the antenna elements to decrease the coupling and to improve the isolation. A study of the various isolation enhancement techniques have been made in this review. It analyses the various isolation enhancement methods such as using orthogonal polarization, parasitic elements, varied decoupling structures, defected ground structures (DGS), neutralization line (NL) and finally by using metamaterials. Metamaterials is a technology to perk up the isolation between the antenna elements. Split ring resonator (SRR) behaves as a metamaterial and it is used as an isolation mechanism in this study. The antennas are simulated and the results are compared. The method using parasitic elements gives the highest isolation of 35 dB and it is 5 dB better than the methods using orthogonal polarization and using the decoupling structure. The performance of all the antennas satisfies the conditions for minimum isolation. The envelope correlation coefficient is nearly zero in all the antennas and it implies good diversity performance. The diversity gain is also calculated for the various antennas and it satisfies good diversity performance. The bandwidth of the antennas is in the UWB frequency range and they have a fractional bandwidth above the required value of 1.09. The capacity loss for all the antennas is very low and the antennas using defected ground structure and the decoupling structure gives very low capacity loss.
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Tan, Gang. "Principles and Implementation Techniques of Software-Based Fault Isolation." Foundations and Trends® in Privacy and Security 1, no. 3 (2017): 137–98. http://dx.doi.org/10.1561/3300000013.

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Yuen, Ho-Chuen, and Ngai-Yin Chan. "Latest technologies and techniques to improve pulmonary vein isolation." International Journal of Heart Rhythm 2, no. 1 (2017): 13. http://dx.doi.org/10.4103/2352-4197.208460.

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Šafařı́k, Ivo, and Mirka Šafařı́ková. "Use of magnetic techniques for the isolation of cells." Journal of Chromatography B: Biomedical Sciences and Applications 722, no. 1-2 (February 1999): 33–53. http://dx.doi.org/10.1016/s0378-4347(98)00338-7.

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