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Journal articles on the topic 'Isotopes labeling'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Kainosho, Masatsune, and Peter Güntert. "SAIL – stereo-array isotope labeling." Quarterly Reviews of Biophysics 42, no. 4 (2009): 247–300. http://dx.doi.org/10.1017/s0033583510000016.

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AbstractOptimal stereospecific and regiospecific labeling of proteins with stable isotopes enhances the nuclear magnetic resonance (NMR) method for the determination of the three-dimensional protein structures in solution. Stereo-array isotope labeling (SAIL) offers sharpened lines, spectral simplification without loss of information and the ability to rapidly collect and automatically evaluate the structural restraints required to solve a high-quality solution structure for proteins up to twice as large as before. This review gives an overview of stable isotope labeling methods for NMR spectr

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Millard, Pierre, Baudoin Delépine, Matthieu Guionnet, Maud Heuillet, Floriant Bellvert, and Fabien Létisse. "IsoCor: isotope correction for high-resolution MS labeling experiments." Bioinformatics 35, no. 21 (2019): 4484–87. http://dx.doi.org/10.1093/bioinformatics/btz209.

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Abstract Summary Mass spectrometry (MS) is widely used for isotopic studies of metabolism and other (bio)chemical processes. Quantitative applications in systems and synthetic biology require to correct the raw MS data for the contribution of naturally occurring isotopes. Several tools are available to correct low-resolution MS data, and recent developments made substantial improvements by introducing resolution-dependent correction methods, hence opening the way to the correction of high-resolution MS (HRMS) data. Nevertheless, current HRMS correction methods partly fail to determine which is

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Rinkel, Jan, and Jeroen S. Dickschat. "Recent highlights in biosynthesis research using stable isotopes." Beilstein Journal of Organic Chemistry 11 (December 9, 2015): 2493–508. http://dx.doi.org/10.3762/bjoc.11.271.

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The long and successful history of isotopic labeling experiments within natural products research has both changed and deepened our understanding of biosynthesis. As demonstrated in this article, the usage of isotopes is not at all old-fashioned, but continues to give important insights into biosynthetic pathways of secondary metabolites. This review with 85 cited references is structured by separate discussions of compounds from different classes including polyketides, non-ribosomal peptides, their hybrids, terpenoids, and aromatic compounds formed via the shikimate pathway. The text does not

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Seeger, Stefan, and Markus Weiler. "Temporal dynamics of tree xylem water isotopes: in situ monitoring and modeling." Biogeosciences 18, no. 15 (2021): 4603–27. http://dx.doi.org/10.5194/bg-18-4603-2021.

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Abstract. We developed a setup for a fully automated, high-frequency in situ monitoring system of the stable water isotope deuterium and 18O in soil water and tree xylem. The setup was tested for 12 weeks within an isotopic labeling experiment during a large artificial sprinkling experiment including three mature European beech (Fagus sylvatica) trees. Our setup allowed for one measurement every 12–20 min, enabling us to obtain about seven measurements per day for each of our 15 in situ probes in the soil and tree xylem. While the labeling induced an abrupt step pulse in the soil water isotopi

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Sonet, Jordan, Anne-Laure Bulteau, Zahia Touat-Hamici, et al. "Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines." International Journal of Molecular Sciences 22, no. 14 (2021): 7308. http://dx.doi.org/10.3390/ijms22147308.

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Selenoproteins, in which the selenium atom is present in the rare amino acid selenocysteine, are vital components of cell homeostasis, antioxidant defense, and cell signaling in mammals. The expression of the selenoproteome, composed of 25 selenoprotein genes, is strongly controlled by the selenium status of the body, which is a corollary of selenium availability in the food diet. Here, we present an alternative strategy for the use of the radioactive 75Se isotope in order to characterize the selenoproteome regulation based on (i) the selective labeling of the cellular selenocompounds with non

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Charron, Craig S., Steven J. Britz, Roman M. Mirecki, Dawn J. Harrison, Beverly A. Clevidence, and Janet A. Novotny. "Isotopic Labeling of Red Cabbage Anthocyanins with Atmospheric 13CO2." Journal of the American Society for Horticultural Science 133, no. 3 (2008): 351–59. http://dx.doi.org/10.21273/jashs.133.3.351.

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Isotopic labeling of plants provides a unique opportunity for understanding metabolic processes. A significant challenge of isotopic labeling during plant growth is that isotopes must be administered without disrupting plant development and at sufficient levels for mass spectral analysis. We describe a system for isotopic labeling of leafy vegetables with 13C and demonstrate successful incorporation of 13C into anthocyanins of preheading red cabbage (Brassica oleracea L. var. capitata L.). ‘Super Red’ red cabbage seedlings were grown for 34 days in an airtight acrylic labeling chamber supplied

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7

Krijgsveld, Jeroen, and Albert J. R. Heck. "Quantitative proteomics by metabolic labeling with stable isotopes." Drug Discovery Today: TARGETS 3, no. 2 (2004): 11–15. http://dx.doi.org/10.1016/s1741-8372(04)02420-x.

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8

Del Vecchio, Antonio, Gianluca Destro, Frédéric Taran, and Davide Audisio. "Recent developments in heterocycle labeling with carbon isotopes." Journal of Labelled Compounds and Radiopharmaceuticals 61, no. 13 (2018): 988–1007. http://dx.doi.org/10.1002/jlcr.3666.

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9

Fernández-del-Río, Lucía, and Catherine F. Clarke. "Coenzyme Q Biosynthesis: An Update on the Origins of the Benzenoid Ring and Discovery of New Ring Precursors." Metabolites 11, no. 6 (2021): 385. http://dx.doi.org/10.3390/metabo11060385.

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Coenzyme Q (ubiquinone or CoQ) is a conserved polyprenylated lipid essential for mitochondrial respiration. CoQ is composed of a redox-active benzoquinone ring and a long polyisoprenyl tail that serves as a membrane anchor. A classic pathway leading to CoQ biosynthesis employs 4-hydroxybenzoic acid (4HB). Recent studies with stable isotopes in E. coli, yeast, and plant and animal cells have identified CoQ intermediates and new metabolic pathways that produce 4HB. Stable isotope labeling has identified para-aminobenzoic acid as an alternate ring precursor of yeast CoQ biosynthesis, as well as o

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Black, G. E., Y. C. Boller, K. A. Kennedy, P. Lecchi, and F. P. Abramson. "Labeling DNA with Stable Isotopes: Economical and Practical Considerations." BioTechniques 30, no. 1 (2001): 134–40. http://dx.doi.org/10.2144/01301rr01.

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11

Seyfried, Martin S., Birgit S. Lauber, and Nathan W. Luedtke. "Multiple-Turnover Isotopic Labeling of Fmoc- and Boc-Protected Amino Acids with Oxygen Isotopes." Organic Letters 12, no. 1 (2010): 104–6. http://dx.doi.org/10.1021/ol902519g.

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12

Schaefer, Juergen R., Daniel J. Rader, and H. Bryan Brewer. "Investigation of lipoprotein kinetics using endogenous labeling with stable isotopes." Current Opinion in Lipidology 3, no. 3 (1992): 227–32. http://dx.doi.org/10.1097/00041433-199206000-00011.

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13

Fox, Atherton, Dainty, et al. "Absorption of Selenium from Wheat, Garlic, and Cod Intrinsically Labeled with Se-77 and Se-82 stable Isotopes." International Journal for Vitamin and Nutrition Research 75, no. 3 (2005): 179–86. http://dx.doi.org/10.1024/0300-9831.75.3.179.

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There is limited information on the absorption of selenium from different foods in humans because of technical difficulties associated with isotopic labeling of dietary selenium. Wheat, garlic, and cod fish were intrinsically labeled with Se-77 or Se-82 stable isotopes. Labeled meals were fed in random order to 14 adults, with a minimum washout period of six weeks between each test meal. Apparent absorption was measured as luminal loss using a fecal monitoring technique over an 8-day period. Plasma appearance of the isotope was measured at 7, 24, and 48 hours post-ingestion. Selenium absorptio

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Sadygov, Rovshan G. "High-Resolution Mass Spectrometry for In Vivo Proteome Dynamics using Heavy Water Metabolic Labeling." International Journal of Molecular Sciences 21, no. 21 (2020): 7821. http://dx.doi.org/10.3390/ijms21217821.

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Cellular proteins are continuously degraded and synthesized. The turnover of proteins is essential to many cellular functions. Combined with metabolic labeling using stable isotopes, LC–MS estimates proteome dynamics in high-throughput and on a large scale. Modern mass spectrometers allow a range of instrumental settings to optimize experimental output for specific research goals. One such setting which affects the results for dynamic proteome studies is the mass resolution. The resolution is vital for distinguishing target species from co-eluting contaminants with close mass-to-charge ratios.

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Serfass, R. E., G. L. Lindberg, J. A. Livares, and R. S. Houk. "Intrinsic Labeling of Bovine Milk with Enriched Stable Isotopes of Zinc." Experimental Biology and Medicine 186, no. 1 (1987): 113–17. http://dx.doi.org/10.3181/00379727-186-1-rc1.

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Marek, Aleš, Blanka Klepetářová, and Tomáš Elbert. "A facile method for steroid labeling by heavy isotopes of hydrogen." Tetrahedron 71, no. 29 (2015): 4874–82. http://dx.doi.org/10.1016/j.tet.2015.04.099.

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Meijs, Wilma E., Hidde J. Haisma, Roel Van Der Schors, et al. "A facile method for the labeling of proteins with zirconium isotopes." Nuclear Medicine and Biology 23, no. 4 (1996): 439–48. http://dx.doi.org/10.1016/0969-8051(96)00020-0.

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18

Patil, Mahadeo R., Tomas Elbert, and Rangappa S. Ker. "ChemInform Abstract: Labeling of Brassinosteroids by Isotopes of Hydrogen and Carbon." ChemInform 46, no. 28 (2015): no. http://dx.doi.org/10.1002/chin.201528287.

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19

Babin, Victor, Antoine Sallustrau, Olivier Loreau, et al. "A general procedure for carbon isotope labeling of linear urea derivatives with carbon dioxide." Chemical Communications 57, no. 54 (2021): 6680–83. http://dx.doi.org/10.1039/d1cc02665h.

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Kabalka, George W., Min-Liang Yao, Murthy Akula, and Li Yong. "Isotope incorporation using organoboranes." Pure and Applied Chemistry 84, no. 11 (2012): 2309–15. http://dx.doi.org/10.1351/pac-con-12-01-13.

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Isotopes have played an important role in chemistry, biology, and medicine. For the last three decades, we have focused on the use of organoboron compounds as precursors to isotopically labeled physiologically active reagents. During that period, we have successfully developed methods for incorporating short- and long-lived isotopes of carbon, nitrogen, oxygen, and the halogens using a variety of reactive organoboron precursors. In addition, labeling strategies employing polymer-supported organoboron derivatives were developed. In this report, we present a short overview focused on the evoluti

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Reichardt, Nicole, Andrew R. Barclay, Lawrence T. Weaver, and Douglas J. Morrison. "Use of Stable Isotopes To Measure the Metabolic Activity of the Human Intestinal Microbiota." Applied and Environmental Microbiology 77, no. 22 (2011): 8009–14. http://dx.doi.org/10.1128/aem.05573-11.

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ABSTRACTThe human intestinal microbiota is a complex biological system comprising a vast repertoire of microbes with considerable metabolic activity relevant to both bacterial growth and host health. Greater strides have been made in the analysis of microbial diversity than in the measurement of functional activity, particularlyin vivo. Stable isotope probing offers a new approach by coupling measurements of metabolic activity with microbial identification. Using a low-enrichment labeling strategyin vitro, this study has identified metabolically active bacterial groups via magnetic-bead captur

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Wu, Christine C., Michael J. MacCoss, Kathryn E. Howell, Dwight E. Matthews, and John R. Yates. "Metabolic Labeling of Mammalian Organisms with Stable Isotopes for Quantitative Proteomic Analysis." Analytical Chemistry 76, no. 17 (2004): 4951–59. http://dx.doi.org/10.1021/ac049208j.

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23

Suzuki, Kazuo T., Layla Somekawa, Kazuki Kurasaki, and Noriyuki Suzuki. "Absolute Labeling and Simultaneous Speciation in Tracer Experiments with Multiple Stable Isotopes." JOURNAL OF HEALTH SCIENCE 52, no. 5 (2006): 590–97. http://dx.doi.org/10.1248/jhs.52.590.

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24

Del Vecchio, Antonio, Alex Talbot, Fabien Caillé, et al. "Carbon isotope labeling of carbamates by late-stage [11C], [13C] and [14C]carbon dioxide incorporation." Chemical Communications 56, no. 78 (2020): 11677–80. http://dx.doi.org/10.1039/d0cc05031h.

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A procedure which allows labelling cyclic carbamates with all carbon isotopes has been developed. This protocol valorizes carbon dioxide, the universal building block for radiolabeling. A series of pharmaceuticals were obtained and a disconnection/reconnection strategy was implemented.

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25

Hasenour, Clinton M., Martha L. Wall, D. Emerson Ridley, et al. "Mass spectrometry-based microassay of 2H and 13C plasma glucose labeling to quantify liver metabolic fluxes in vivo." American Journal of Physiology-Endocrinology and Metabolism 309, no. 2 (2015): E191—E203. http://dx.doi.org/10.1152/ajpendo.00003.2015.

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Mouse models designed to examine hepatic metabolism are critical to diabetes and obesity research. Thus, a microscale method to quantitatively assess hepatic glucose and intermediary metabolism in conscious, unrestrained mice was developed. [13C3]propionate, [2H2]water, and [6,6-2H2]glucose isotopes were delivered intravenously in short- (9 h) and long-term-fasted (19 h) C57BL/6J mice. GC-MS and mass isotopomer distribution (MID) analysis were performed on three 40-μl arterial plasma glucose samples obtained during the euglycemic isotopic steady state. Model-based regression of hepatic glucose

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Cabin-Flaman, Armelle, Anne-Francoise Monnier, Yannick Coffinier, et al. "Combining combing and secondary ion mass spectrometry to study DNA on chips using 13C and 15N labeling." F1000Research 5 (June 20, 2016): 1437. http://dx.doi.org/10.12688/f1000research.8361.1.

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Dynamic secondary ion mass spectrometry (D-SIMS) imaging of combed DNA – the combing, imaging by SIMS or CIS method – has been developed previously using a standard NanoSIMS 50 to reveal, on the 50 nm scale, individual DNA fibers labeled with different, non-radioactive isotopes in vivo and to quantify these isotopes. This makes CIS especially suitable for determining the times, places and rates of DNA synthesis as well as the detection of the fine-scale re-arrangements of DNA and of molecules associated with combed DNA fibers. Here, we show how CIS may be extended to 13C-labeling via the detec

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Zou, Juan, A. Neil Turner, and Richard G. Phelps. "Trace Labeling of Proteins with Stable Isotopes To Identify Fragments in Complex Mixtures." Analytical Chemistry 76, no. 5 (2004): 1445–52. http://dx.doi.org/10.1021/ac035160i.

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28

Veillon, C., K. Y. Patterson, L. N. Button, and A. J. Sytkowski. "Selenium utilization in humans--a long-term, self-labeling experiment with stable isotopes." American Journal of Clinical Nutrition 52, no. 1 (1990): 155–58. http://dx.doi.org/10.1093/ajcn/52.1.155.

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Meermann, Björn, Kristina Wichmann, Franziska Lauer, Frank Vanhaecke, and Thomas A. Ternes. "Application of stable isotopes and AF4/ICP-SFMS for simultaneous tracing and quantification of iron oxide nanoparticles in a sediment–slurry matrix." Journal of Analytical Atomic Spectrometry 31, no. 4 (2016): 890–901. http://dx.doi.org/10.1039/c5ja00383k.

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A new analytical approach was developed by us allowing for unambiguous tracing and simultaneous quantification of Fe-oxide ENPs in the presence of a natural iron colloid matrix. The approach relies on isotope labeling of ENPs and reverse post-channel species-unspecific on-line isotope dilution in combination with AF4/ICP-SFMS.

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Baskin, D. G., and J. F. Breininger. "Colocalization of mRNAs by Fluorescence in Situ Hybridization." Microscopy and Microanalysis 5, S2 (1999): 480–81. http://dx.doi.org/10.1017/s1431927600015725.

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Fluorescence in situ hybridization (FISH), long the method of choice for chromosomal cytogenetics, is becoming recognized as a powerful method for correlative histochemical detection of multiple messenger ribonucleic acid (mRNA) in cells. The technique is based upon the principle of the binding of a labeled strand of DNA (an oligonucleotide probe) or RNA (a riboprobe) to complementary strands of mRNA. In the traditional in situ hybridization method, nucleic acid probes are labeled with radioactive isotopes and the hybrids are localized by autoradiography. More recently, labeling of the probes

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Birkemeyer, Claudia, Alexander Luedemann, Cornelia Wagner, Alexander Erban, and Joachim Kopka. "Metabolome analysis: the potential of in vivo labeling with stable isotopes for metabolite profiling." Trends in Biotechnology 23, no. 1 (2005): 28–33. http://dx.doi.org/10.1016/j.tibtech.2004.12.001.

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32

Bindschedler, L. V., and R. Cramer. "Fully automated software solution for protein quantitation by global metabolic labeling with stable isotopes." Rapid Communications in Mass Spectrometry 25, no. 11 (2011): 1461–71. http://dx.doi.org/10.1002/rcm.4872.

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Bontes, B. M., R. Pel, B. W. Ibelings, H. T. S. Boschker, J. J. Middelburg, and E. Van Donk. "The effects of biomanipulation on the biogeochemistry, carbon isotopic composition and pelagic food web relations of a shallow lake." Biogeosciences 3, no. 1 (2006): 69–83. http://dx.doi.org/10.5194/bg-3-69-2006.

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Abstract. In this study we investigated the effects of experimental biomanipulation on community structure, ecosystem metabolism, carbon biogeochemistry and stable isotope composition of a shallow eutrophic lake in the Netherlands. Three different biomanipulation treatments were applied. In two parts of the lake, isolated from the rest, fish was removed and one part was used as a reference treatment in which no biomanipulation was applied. Stable isotopes have proved useful to trace trophic interactions at higher food web levels but until now methodological limitations have restricted species

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Beyer, M., J. T. Hamutoko, H. Wanke, M. Gaj, and P. Koeniger. "Examination of deep root water uptake using anomalies of soil water stable isotopes, depth-controlled isotopic labeling and mixing models." Journal of Hydrology 566 (November 2018): 122–36. http://dx.doi.org/10.1016/j.jhydrol.2018.08.060.

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35

Hood-Nowotny, Rebecca. "Using light stable isotopes to understand nutrient cycling in soils and how these isotopic techniques can be leveraged to investigate the ecology and biology of insects – A review." Die Bodenkultur: Journal of Land Management, Food and Environment 68, no. 4 (2018): 237–48. http://dx.doi.org/10.1515/boku-2017-0019.

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Summary At first glance, there appears little to link nitrogen cycling with entomology other that the use of isotope techniques. Soil management requires a contextual, adaptive, flexible approach that is based on understanding the factors that regulate the soil’s fundamental processes. Using stable isotope techniques for the analysis of the inherent biogeochemical processes can explain the complex soil–plant interactions, the determining factors of the nitrogen cycle, and the impacts of applying external inputs. Using the same stable isotope tools enables an interdisciplinary collaboration bet

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Ermolaev, Stanislav, Aino Skasyrskaya, and Aleksandr Vasiliev. "A Radionuclide Generator of High-Purity Bi-213 for Instant Labeling." Pharmaceutics 13, no. 6 (2021): 914. http://dx.doi.org/10.3390/pharmaceutics13060914.

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A new two-column 225Ac/213Bi generator was developed specifically for using 225Ac containing an impurity of long lived 227Ac. The parent 225Ac was retained on the first Actinide Resin column, while 213Bi was accumulated on the second column filled with AG MP-50 resin via continuous elution and decay of intermediate 221Fr. The 213Bi accumulation was realized in circulation mode which allowed a compact generator design. It was demonstrated that 213Bi could be quickly and effectively extracted from AG MP-50 in form of complexes with various chelating agents including DTPA and DOTA. The performanc

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Vucina, Jurij, and Ruben Han. "Production and therapeutic application of rhenium isotopes, rhenium-186 and rhenium-188: Radioactive pharmaceuticals of the future." Medical review 56, no. 7-8 (2003): 362–65. http://dx.doi.org/10.2298/mpns0308362v.

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Introduction In contemporary nuclear medicine, alpha, pure beta or beta-gamma emitters are used for targeted therapy. Use of pure and combined alpha/beta emitters in oncology, endocrinology, rheumatology and, a short while ago, interventional cardiology, has refined as an important alternative to more common therapeutic regimens. Two radioisotopes of rhenium, rhenium-186 and rhenium-188, are of particular interest. Production of Rhenium-186 and Rhenium-188 Rhenium-186 is routinely produced in nuclear reactors by direct neutron activation of metallic rhenium enriched with 185Re via 185Re(n,)186

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Bartram, Michael E., and J. Randall Creighton. "GaN CVD Reactions: Hydrogen and Ammonia Decomposition and the Desorption of Gallium." MRS Internet Journal of Nitride Semiconductor Research 4, S1 (1999): 369–74. http://dx.doi.org/10.1557/s109257830000274x.

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Isotopic labeling experiments have revealed correlations between hydrogen reactions, Ga desorption, and ammonia decomposition in GaN CVD. Low energy electron diffraction (LEED) and temperature programmed desorption (TPD) were used to demonstrate that hydrogen atoms are available on the surface for reaction after exposing GaN(0001) to deuterium at elevated temperatures. Hydrogen reactions also lowered the temperature for Ga desorption significantly. Ammonia did not decompose on the surface before hydrogen exposure. However, after hydrogen reactions altered the surface, N15H3 did undergo both re

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Malik, Sohail, Margaret Kenny, George Doss, and Varghese John. "A novel one step approach to small scale labeling of organic compounds with hydrogen isotopes." Journal of Labelled Compounds and Radiopharmaceuticals 34, no. 5 (1994): 471–73. http://dx.doi.org/10.1002/jlcr.2580340510.

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Stockley, Paula, Catarina Franco, Amy J. Claydon, et al. "Revealing mechanisms of mating plug function under sexual selection." Proceedings of the National Academy of Sciences 117, no. 44 (2020): 27465–73. http://dx.doi.org/10.1073/pnas.1920526117.

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Mating plugs are produced by many sexually reproducing animals and are hypothesized to promote male fertilization success under promiscuous mating. However, tests of this hypothesis have been constrained by an inability to discriminate ejaculates of different males in direct competition. Here, we use stable isotope labeling in vivo and proteomics to achieve this in a promiscuous rodent,Myodes glareolus. We show that, although the first male’s plug is usually dislodged, it can be retained throughout the second male’s copulation. Retained plugs did not completely block rival sperm but did signif

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Wallace, Carmichael JA, and Ian Clark-Lewis. "Site-specific independent double labeling of proteins with reporter atoms." Biochemistry and Cell Biology 78, no. 2 (2000): 79–86. http://dx.doi.org/10.1139/o00-001.

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Many types of physical, spectroscopic, and biological studies of proteins and other macromolecules are facilitated by the incorporation of reporter groups. In many cases these are single atom substitutes, for example isotopes (13C for C), or light (F for H) and heavy (Se for S) atom homologs. In some circumstances the incorporation of two different labels in the same molecule would be greatly desirable. Commonly used protein engineering methods for incorporating them can rarely cope with differential double labeling, and have other limitations such as universal, non-specific, or random incorpo

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Bąchor, Remigiusz, Mateusz Waliczek, Piotr Stefanowicz, and Zbigniew Szewczuk. "Trends in the Design of New Isobaric Labeling Reagents for Quantitative Proteomics." Molecules 24, no. 4 (2019): 701. http://dx.doi.org/10.3390/molecules24040701.

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Modern mass spectrometry is one of the most frequently used methods of quantitative proteomics, enabling determination of the amount of peptides in a sample. Although mass spectrometry is not inherently a quantitative method due to differences in the ionization efficiency of various analytes, the application of isotope-coded labeling allows relative quantification of proteins and proteins. Over the past decade, a new method for derivatization of tryptic peptides using isobaric labels has been proposed. The labels consist of reporter and balanced groups. They have the same molecular weights and

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van Manen, Henk-Jan, Aufried Lenferink, and Cees Otto. "Noninvasive Imaging of Protein Metabolic Labeling in Single Human Cells Using Stable Isotopes and Raman Microscopy." Analytical Chemistry 80, no. 24 (2008): 9576–82. http://dx.doi.org/10.1021/ac801841y.

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Ouguerram, K., M. Krempf, C. Maugeais, P. Maug[egrave]re, D. Darmaun, and T. Magot. "A new labeling approach using stable isotopes to study in vivo plasma cholesterol metabolism in humans." Metabolism 51, no. 1 (2002): 5–11. http://dx.doi.org/10.1053/meta.2002.29006.

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Hong, Hao, Jiangtao Sun, and Weibo Cai. "Radionuclide-Based Cancer Imaging Targeting the Carcinoembryonic Antigen." Biomarker Insights 3 (January 2008): BMI.S1124. http://dx.doi.org/10.4137/bmi.s1124.

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Carcinoembryonic antigen (CEA), highly expressed in many cancer types, is an important target for cancer diagnosis and therapy. Radionuclide-based imaging techniques (gamma camera, single photon emission computed tomography [SPECT] and positron emission tomography [PET]) have been extensively explored for CEA-targeted cancer imaging both preclinically and clinically. Briefly, these studies can be divided into three major categories: antibody-based, antibody fragment-based and pretargeted imaging. Radiolabeled anti-CEA antibodies, reported the earliest among the three categories, typically gave

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Tessier, Romain, Nadezda Khodorova, Juliane Calvez, et al. "15N and ²H Intrinsic Labeling Demonstrate That Real Digestibility in Rats of Proteins and Amino Acids from Sunflower Protein Isolate Is Almost as High as That of Goat Whey." Journal of Nutrition 150, no. 3 (2019): 450–57. http://dx.doi.org/10.1093/jn/nxz279.

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ABSTRACT Background In the context of developing plant protein sources for humans, sunflower is a good candidate in its form as an oilseed coproduct. Objectives We aimed to compare the real digestibility in rats of a sunflower isolate to that of goat whey protein. We also studied the efficiency of 15N and 2H intrinsic labeling in this assessment. Methods Sunflower seeds and goat milk were labeled with 15N and 2H. Male Wistar rats (10 wk old) were fed a meal containing 12% of either sunflower isolate (n = 8) or whey (n = 8). Six hours after meal ingestion, protein and amino acid digestibility w

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Shih, Lu-Min, Hsiang-Yu Tang, Ke-Shiuan Lynn, Cheng-Yu Huang, Hung-Yao Ho, and Mei-Ling Cheng. "Stable Isotope-Labeled Lipidomics to Unravel the Heterogeneous Development Lipotoxicity." Molecules 23, no. 11 (2018): 2862. http://dx.doi.org/10.3390/molecules23112862.

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Non-alcoholic fatty liver disease (NAFLD) as a global health problem has clinical manifestations ranging from simple non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH), cirrhosis, and cancer. The role of different types of fatty acids in driving the early progression of NAFL to NASH is not understood. Lipid overload causing lipotoxicity and inflammation has been considered as an essential pathogenic factor. To correlate the lipid profiles with cellular lipotoxicity, we utilized palmitic acid (C16:0)- and especially unprecedented palmitoleic acid (C16:1)-induced lipid over

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Allen, Lindsay Helen. "Bioavailability of Vitamin B12." International Journal for Vitamin and Nutrition Research 80, no. 45 (2010): 330–35. http://dx.doi.org/10.1024/0300-9831/a000041.

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Vitamin B12 deficiency is common in people of all ages who consume a low intake of animal-source foods, including populations in developing countries. It is also prevalent among the elderly, even in wealthier countries, due to their malabsorption of B12 from food. Several methods have been applied to diagnose vitamin B12 malabsorption, including Schillings test, which is now used rarely, but these do not quantify percent bioavailability. Most of the information on B12 bioavailability from foods was collected 40 to 50 years ago, using radioactive isotopes of cobalt to label the corrinoid ring.

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Starrs, D., B. C. Ebner, S. M. Eggins, and C. J. Fulton. "Longevity in maternal transmission of isotopic marks in a tropical freshwater rainbowfish and the implications for offspring morphology." Marine and Freshwater Research 65, no. 5 (2014): 400. http://dx.doi.org/10.1071/mf13150.

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Transgenerational marking is increasingly being used to study the early life history, biology and ecology of fishes. However, the timeframe over which the injected enriched stable isotopes remain in the mother and are passed onto her offspring is largely unknown. Similarly, we have relatively little knowledge of the effects of isotope labelling on the morphology of offspring. In this study, we injected adult female eastern rainbowfish (Melanotaenia splendida) with two doses (20 µg g–1 and 40 µg g–1) of enriched 137Ba or 87Sr stable isotopes to mark the otoliths of their larvae and examine the

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Moon, Euy Sung, Yentl Van Rymenant, Sandeep Battan, et al. "In Vitro Evaluation of the Squaramide-Conjugated Fibroblast Activation Protein Inhibitor-Based Agents AAZTA5.SA.FAPi and DOTA.SA.FAPi." Molecules 26, no. 12 (2021): 3482. http://dx.doi.org/10.3390/molecules26123482.

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Recently, the first squaramide-(SA) containing FAP inhibitor-derived radiotracers were introduced. DATA5m.SA.FAPi and DOTA.SA.FAPi with their non-radioactive complexes showed high affinity and selectivity for FAP. After a successful preclinical study with [68Ga]Ga-DOTA.SA.FAPi, the first patient studies were realized for both compounds. Here, we present a new squaramide-containing compound targeting FAP, based on the AAZTA5 chelator 1,4-bis-(carboxylmethyl)-6-[bis-(carboxymethyl)-amino-6-pentanoic-acid]-perhydro-1,4-diazepine. For this molecule (AAZTA5.SA.FAPi), complexation with radionuclides

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