Academic literature on the topic 'Isozyme polymorphism'

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Journal articles on the topic "Isozyme polymorphism"

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ANIEL KUMAR, Owk, Sape S. TATA, and Kancharla PAVAN KUMAR. "Analysis of Several Popular Cultivars of Madagascar Periwinkle (Catharanthus roseus (L.) G. Don.) using Biochemical Markers." Notulae Scientia Biologicae 5, no. 4 (2013): 458–61. http://dx.doi.org/10.15835/nsb549178.

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Band designs of esterase (EST), peroxidase (PO) and polyphenol oxidase (PPO) isozymes in several selected cultivars of Catharanthus roseus by using native polyacrylamide gel electrophoresis (PAGE) were investigated in this study. It was confirmed that cultivar differences in isozyme polymorphism can be revealed by applied electrophoretic patterns. Three isozyme systems produced a total of 16 bands with polymorphism ranged from 66.6-100%. Considering the patterns of isozyme variations in the five cultivars of Catharanthus roseus, it is evident that the cultivar ‘First kiss coral’ displayed crim
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WEEDEN, N. F., and A. C. EMMO. "ISOZYME CHARACTERIZATION OF KENTUCKY BLUEGRASS CULTIVARS." Canadian Journal of Plant Science 65, no. 4 (1985): 985–94. http://dx.doi.org/10.4141/cjps85-126.

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Twenty-two cultivars of Kentucky bluegrass (Poa pratensis L.) were examined for their glucose phosphate isomerase, triose phosphate isomerase, phosphoglucomutase, malate dehydrogenase, 6-phosphogluconate dehydrogenase and alpha-naphthyl esterase phenotypes. Polymorphism was observed in each isozyme system, and the first four systems were characterized for variation among and within the cultivars. Sixteen of the 22 cultivars could be uniquely identified by selecting isozymes which displayed clearly resolved bands and exhibited little or no intracultivar polymorphism. Analysis could be performed
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Degani, Chemda, Ruth El-Batsri, and Shmuel Gazit. "Enzyme Polymorphism in Mango." Journal of the American Society for Horticultural Science 115, no. 5 (1990): 844–47. http://dx.doi.org/10.21273/jashs.115.5.844.

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Forty-one (Mangifera indica L.) cultivars were characterized electrophoretically using the isozyme systems aconitase, isocitrate dehydrogenase, leucine aminopeptidase, phosphoglucose isomerase, phosphoglucomutase, and triosephosphate isomerase. The outcross origin of some of the mango cultivars was supported by the isozymic banding patterns. Reported parentage of some other cultivars was not consistent with their isozymic banding patterns.
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Samimy, Cyrus, and James N. Cummins. "Distinguishing Apple Rootstocks by Isozyme Banding Patterns." HortScience 27, no. 7 (1992): 829–31. http://dx.doi.org/10.21273/hortsci.27.7.829.

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Isozymes of six enzyme systems extracted from 13 apple (Malus domestica Borkh.) rootstocks were separated electrophoretically on a horizontal starch gel. Each rootstock was clearly distinguished by its unique isozyme banding patterns. All the rootstocks were distinguishable using only two of the enzyme systems, phosphoglucomutase and 6-phosphogluconate dehydrogenase, both of which exhibited considerable isozyme polymorphism.
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Haque, ME, MA Islam, and B. Sikdar. "Molecular divergence of locally grown pumpkin (Cucurbita maxima) cultivars through some isozyme tests." Journal of Bio-Science 19 (December 19, 2012): 89–93. http://dx.doi.org/10.3329/jbs.v19i0.13006.

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Context: Pumpkin (Cucurbita maxima) is highly polymorphic vegetable species and its polymorphism can be analyzed by isozyme molecular marker. Objective: To analyze genetic polymorphism among 10 locally grown pumpkin cultivars by isozyme. Materials and Methods: Fresh leaves of young plant of different cultivars were used for enzyme extraction. Enzyme extracts were prepared by homogenizing of 2 g sample of each cultivar. Prechilled mortar and pestle nestled in ice along with 1% polyvinylpyrrolidone and 2 ml of chilled extraction buffer were used prior to centrifuge. N-PAGE was conducted for diff
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Degani, C., A. Beiles, R. El-Batsri, M. Goren, and S. Gazit. "Identifying Lychee Cultivars by Isozyme Analysis." Journal of the American Society for Horticultural Science 120, no. 2 (1995): 307–12. http://dx.doi.org/10.21273/jashs.120.2.307.

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Leaf isozyme banding patterns were studied in 30 cultivars and selections of lychee (Litchi Chinensis Sonn.) by means of starch gel electrophoresis. Polymorphism in aconitase, aspartate aminotransferase, isocitrate dehydrogenase, phosphoglucomutase, shikimate dehydrogenase, superoxide dismutase and triosephosphate isomerase is demonstrated for the first time and observations are extended for the previously described polymorphism in phosphoglucose isomerase. In this study we found five groups of cultivars with identical electrophoretic genotypes. The 18 different cultivars were clustered by the
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Ipek, Meryem, Ahmet Ipek, and Philipp W. Simon. "Comparison of AFLPs, RAPD Markers, and Isozymes for Diversity Assessment of Garlic and Detection of Putative Duplicates in Germplasm Collections." Journal of the American Society for Horticultural Science 128, no. 2 (2003): 246–52. http://dx.doi.org/10.21273/jashs.128.2.0246.

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Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diversity. Studies which have assessed garlic diversity with isozymes and randomly amplified polymorphic DNA (RAPD) markers generally agreed with the morphological observations but sometimes failed to discriminate clones. To discriminate among closely related garlic clones in more detail, we introduced amplified fragment-length polymorphism (AFLPs) to evaluate the genetic diversity and phenetic relatedness of 45 garlic clones and three A. longicuspis clones and we compared AFLP results with RAPD markers
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Ipek, Meryem, Ahmet Ipek, and Philipp W. Simon. "Comparison of AFLPs, RAPD Markers, and Isozymes for Diversity Assessment of Garlic and Detection of Putative Duplicates in Germplasm Collections." Journal of the American Society for Horticultural Science 128, no. 2 (2003): 246–52. https://doi.org/10.21273/jashs.128.2.246.

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Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diversity. Studies which have assessed garlic diversity with isozymes and randomly amplified polymorphic DNA (RAPD) markers generally agreed with the morphological observations but sometimes failed to discriminate clones. To discriminate among closely related garlic clones in more detail, we introduced amplified fragment-length polymorphism (AFLPs) to evaluate the genetic diversity and phenetic relatedness of 45 garlic clones and three A. longicuspis clones and we compared AFLP results with RAPD markers
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Weeden, Norman F., Bruce I. Reisch, and Mary-Howell E. Martens. "Genetic Analysis of Isozyme Polymorphism in Grape." Journal of the American Society for Horticultural Science 113, no. 5 (1988): 765–69. http://dx.doi.org/10.21273/jashs.113.5.765.

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Abstract Genetic analysis of 11 allozyme polymorphisms was performed on the progeny of ‘Cayuga White’ × ‘Aurora’, two complex interspecific grape (Vitis) hybrids. Segregation for most of the polymorphisms closely approximated monogenic Mendelian ratios, and eight new isozyme loci were defined for grape. Joint segregation analysis among the isozyme loci revealed three multilocus linkage groups. These results demonstrate that sufficient allozyme polymorphism exists in grape to establish many multilocus linkage groups and that this genetic analysis can be accomplished using extant progeny or prog
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Sun, Gen-Lou, Oscar Díaz, Björn Salomon, and Roland von Bothmer. "Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers." Genome 42, no. 3 (1999): 420–31. http://dx.doi.org/10.1139/g98-130.

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Genetic diversity of 33 Elymus caninus accessions was investigated using isozyme, RAPD, and microsatellite markers. The three assays differed in the amount of polymorphism detected. Microsatellites detected the highest polymorphism. Six microsatellite primer pairs generated a total of 74 polymorphic bands (alleles), with an average of 15.7 bands per primer pair. Three genetic similarity matrices were estimated based on band presence or absence. Genetic diversity trees (dendrograms) were derived from each marker technique, and compared using Mantel's test. The correlation coefficients were 0.20
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Dissertations / Theses on the topic "Isozyme polymorphism"

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Burges, Nichole. "Characterisation of Rhizoctonia solani isolates from potato in Western Australia using Anastomosis groupings, Pectic Isozyme Groupings, Polymorphism of the ITS Region of the rRNA." Thesis, Burges, Nichole (2002) Characterisation of Rhizoctonia solani isolates from potato in Western Australia using Anastomosis groupings, Pectic Isozyme Groupings, Polymorphism of the ITS Region of the rRNA. Honours thesis, Murdoch University, 2002. https://researchrepository.murdoch.edu.au/id/eprint/32630/.

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The basidiomycete fungus, Rhizoctonia solani is a complex soilborne plant pathogen. As a species R. solani is comprised of many strains, which differ in host specificity as well as physiological characteristics such as colony morphology. R. solani is a major pathogen of the cultivated potato, Solanum tuberosum, and causes a number of economically important diseases worldwide. The aim of this study was to characterise R. solani isolates associated with diseases of potatoes in Western Australia. A hierarchical and more general survey of the major potato growing regions across the state were
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Kongkiatngam, Prasert. "Genetic studies of red clover (Trifolium pratense L.) using morphological, isozyme and random amplified polymorphic DNA (RAPD) markers." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29066.

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Genetic variation within and between two cultivars of red clover (Trifolium pratense L.), Essi from Europe and Ottawa from Canada was estimated using morphological, isozyme and random amplified polymorphic DNA (RAPD) markers. A total of 21 enzyme-coding loci with 43 alleles was detected using twelve enzyme systems. The mean number of alleles per locus was 1.81 in Essi and 1.67 in Ottawa. Nine 10-mer primers were used to assay 20 individuals from each cultivar for RAPD markers. Each primer gave from 7 to 20 amplified bands with an average of 14.8 bands per primer. High within-cultivar variation
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Akromah, Richard. "Isozymes, DNA polymorphisms and stigma morphology in the study of species relationships and evolution of the cultivated peanut (Arachis hypogaea L.)." Thesis, University of Reading, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267413.

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Triest, Ludwig. "Isozymes in water plants : molecular systematics and biogeography of alisma, baldellia, hydrilla, lagarosiphon, potamogeton, ruppia, zannichellia, najas and the seagrasses : enzyme polymorphism and its relationships to biological features in aquatic macrophytes (including a comparison with terrestrial plants) : conservation of genetic diversity /." Meise : National botanic garden of Belgium, 1991. http://catalogue.bnf.fr/ark:/12148/cb41157870w.

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O'Leary, Maureen Catherine. "Isozyme polymorphism and inheritance in Hatiora and Schlumbergera (Cactaceae)." 1996. https://scholarworks.umass.edu/dissertations/AAI9639012.

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Isozyme analysis was used to identify clones, measure levels of genetic variation within groups of clones, and analyze mating systems in two Cactaceae genera--Hatiora and Schlumbergera. Isozymes were extracted from phylloclades and pollen and were separated by polyacrylamide gel electrophoresis. The inheritance of aspartate aminotransferase (AAT), glucose-6-phosphate isomerase (GPI), malate dehydrogenase (MDH), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI) was examined in Hatiora. Six loci (Aat-1, Gpi-1, Mdh-1, Pgm-1, Pgm-2, and Tpi-2) were analyzed, and results were generally
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Wang, Jau-Yueh, and 王昭月. "Identifications of Capsicum spp. by Isozymes and Randomly Amplified Polymorphic DNA (RAPD) Markers." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/94172284351782431844.

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碩士<br>國立中興大學<br>園藝學系<br>83<br>Morphological traits,isozymes and randomly amplified polymor- phic DNA (RAPD) markers were used to study the identification of Capsicum species.The purposes of this study were to establish molecular markers for classification of the Capsicum spp., to establish the genetic linkage relationships and a quick, technique for the identification of seed purity of Capsicum spp. The results of isozyme analysis indicated that there were significantly different in the zy
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Wang, Zhao-Yue, and 王昭月. "Identifications of capsicum spp. by isozymes and randomly amplified polymorphic DNA (RAPD) markers." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/25557591573302930114.

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Books on the topic "Isozyme polymorphism"

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Ludwig, Triest, and Jardin botanique national de Belgique., eds. Isozymes in water plants: Molecular systematics and biogeography of Alisma, Baldellia, Hydrilla, Lagarosiphon, Potamogeton, Ruppia, Zannichellia, Najas and the seagrasses : enzyme polymorphism and its relationships to biological features in aquatic macrophytes (including a comparison with terrestrial plants) : conservation of genetic diversity. National Botanic Garden of Belgium, 1991.

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Liou, Pan-Chi. A molecular study of the citrus genome through restriction fragment length polymorphism and isozyme mapping. 1990.

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Tahir, Muhammad. Use of isozyme polymorphisms in lentil (LENS· culinaris Medik.) for gene mapping and detection of quantitative trait loci. 1990.

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Book chapters on the topic "Isozyme polymorphism"

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Scaltsoyiannes, A. "Isozyme Polymorphism at the 6-Phosphoglucose Dehydrogenase (6Pgd) and Leucine Aminopeptidase (Lap) Loci in 18 Populations of Cedar Species." In Progress in Botanical Research. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5274-7_128.

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Triest, Ludwig, and Bart De Greef. "The use of isozymes and PCR-based DNA polymorphism in aquatic weed management: a case-study on introduced and native clones of a hybrid complex." In Biology, Ecology and Management of Aquatic Plants. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-017-0922-4_12.

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Schreiber, Arnd, and Herbert Tichy. "MHC polymorphisms and the conservation of endangered species." In Biotechnology and the Conservation of Genetic Diversity. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780198540304.003.0009.

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Abstract Southern blot analysis using cloned sequences from the major histocompatibility genes of the human and the chimpanzee revealed extensive MHC polymorphisms in several mammals of conservation interest. Hybridization patterns were obtained with class I and class II probes in all 27 ungulate and primate species tested. Despite their high variability, a partial conservation of MHC restriction fragment patterns suggests a trans-specific mode of evolution of this polymorphism in ruminants, which might permit the differentiation of taxa that are little differentiated at isozyme level and the
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Atanasova Staykova, Teodora, and Panomir Ivanov Tzenov. "Population genetic studies of silkworm (Bombyx mori L.) and phylogenetic relationships." In Lepidoptera - Recent Advancements [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.1002229.

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The study aimed to evaluate the degree of genetic variability and phylogenetic relationships between 13 breeds of mulberry silkworm (Bombyx mori L.) from genetic resources of Bulgaria through isozyme polymorphism. PAGE was used. Among nine studied isoenzyme loci, by eight loci (Bes A, Bes B, Bes D, Bes E, Pgm A, Mdh A, Bph, and Alp A) we found intra-breed and inter-breed polymorphism. At the Hk locus, we found inter-breed polymorphism only. The number of alleles per polymorphic locus ranged from one to two. The degree of polymorphism ranged from 0% to 77.80%. Low levels of observed heterozygos
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GLASZMANN, J. C. "A VARIETAL CLASSIFICATION OF ASIAN CULTIVATED RICE (Oryza sativaL.) BASED ON ISOZYME POLYMORPHISM." In Rice Genetics Collection. World Scientific Publishing Company, 2008. http://dx.doi.org/10.1142/9789812814265_0008.

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Gabriel, Dean W., and Robert De Feyter. "RFLP analyses and gene tagging for bacterial identification and taxonomy." In Molecular Plant Pathology. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199631032.003.0006.

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Abstract Accurate identification of bacterial isolates to species, subspecies, and pathovar groups coupled with an objective bacterial taxonomy are of paramount importance to microbiologists and plant pathologists. Highly refined genetic technologies facilitate high resolution distinctions such that taxonomies based on phenotype (phenetic taxonomies) are proving to be artificial by genetic standards. The availability of powerful analytical tools such as DNA sequencing, DNA-DNA hybridizations, multilocus isozyme analyses, and restriction fragment length polymorphism (RFLP) hybridization analyse
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Fritsch, Peter, and Loren H. Rieseberc. "The Use of Random Amplified Polymorphic DNA (RAPD) in Conservation Genetics." In Molecular Genetic Approaches in Conservation. Oxford University PressNew York, NY, 1996. http://dx.doi.org/10.1093/oso/9780195095265.003.0004.

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Abstract A primary goal of conservation genetics is to estimate the level and distribution of genetic variation in endangered species. Genetic data are often critical to understanding the structure, evolutionary relationships, demographics, and taxonomy of populations, races, and species; thus, they often play a significant role in the formulation of appropriate management strategies directed toward the conservation of taxa (Beardmore, 1983; Allendorf and Leary, 1986; Lacy, 1988). Currently, the most popular and cost-effective means by which conservation geneticists infer variation at the mole
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Georgiadis, Nicholas. "Reconstructing Population History Using PCR-Restriction Site Data." In Molecular Genetic Approaches in Conservation. Oxford University PressNew York, NY, 1996. http://dx.doi.org/10.1093/oso/9780195095265.003.0010.

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Abstract Many recent advances in genetic technology have had almost instantaneous applications to conservation, as this volume attests. Examples include a growing variety of genetic markers, accessible with the polymerase chain reaction (PCR), that are suitable for forensic detection, and the definition of systematically inclusive “units” for conservation at population and species levels (Morin et al., 1994, Moritz 1994, Volgler and de Salle, 1994). While many of these markers are informative in the sense that their presence or absence is diagnostic, or that degrees of phenetic similarity can
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Conference papers on the topic "Isozyme polymorphism"

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Рудакова, Анжела, Сергей Рудаков та Юрий Чесноков. "Изучение полиморфизма эстеразных изоферментов в семенах картирующей популяции яровой мягкой пшеницы". У VIIth International Scientific Conference “Genetics, Physiology and Plant Breeding”. Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2021. http://dx.doi.org/10.53040/gppb7.2021.44.

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Using electrophoresis, in 114 recombinant hybrid lines of the mapping population of spring bread wheat and in 2 parental forms, 7 esterase isoenzymes were found: A1-A7 (Mr 93-138 kDa). According to their esterase composition, all samples were subdivided into 17 zymotypes. Isoforms A4 and A6 are pre-sent in all zymotypes, i.e. are monomorphic. The other 5 isozymes provide a high level of polymorphism in the population. The majority of genotypes belong to the zymotype Gr. 1 (27%), which includes 6 isoforms. Among them there are isoforms A1 and A7, characteristic only for each of the parent forms
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Reports on the topic "Isozyme polymorphism"

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Reisch, Bruce, Pinhas Spiegel-Roy, Norman Weeden, Gozal Ben-Hayyim, and Jacques Beckmann. Genetic Analysis in vitis Using Molecular Markers. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613014.bard.

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Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and be
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