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Journal articles on the topic 'Itraplasmatic injection of spermatozoa into the oocyte'

Author: Grafiati

Published: 3 June 2025

Last updated: 19 July 2025

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1

Abramova, S., and D. Korobkov. "Survey look at the problem of subsidiary reproductive technologies." Bulletin of Science and Practice, no. 8 (August 15, 2017): 120–27. https://doi.org/10.5281/zenodo.842907.

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This review the problems of modern reproductive technologies. Due to the global introduction and application of assisted reproductive technologies that contribute to the restoration of fertility in infertile couples and allow the realization of the function of procreation in various diseases that until recently were considered completely incompatible with the onset of pregnancy is one of the topical problems of reproductive medicine. The problem of restoring human fertility has largely stimulated the development of new directions in assisted reproductive technologies, which include in vitro fe

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2

Kasai, T., K. Hoshi, and R. Yanagimachi. "Effect of sperm immobilisation and demembranation on the oocyte activation rate in the mouse." Zygote 7, no. 3 (1999): 187–93. http://dx.doi.org/10.1017/s0967199499000568.

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To analyse the effect of the state of the sperm plasma membrane on oocyte activation rate following intracytoplasmic sperm injection (ICSI), three types of human and mouse spermatozoa (intact, immobilised and Triton X-100 treated) were individually injected into mouse oocytes. At 30, 60 and 120 min after injection, maternal chromosomes and sperm nuclei within oocytes were examined. Following human sperm injection, the fastest and the most efficient oocyte activation and sperm head decondensation occurred when the spermatozoa were treated with Triton X-100. Intact spermatozoa were the least eff

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3

Yazawa, Hiroyuki, Kaoru Yanagida, Shoutaro Hayashi, and Akira Sato. "The oocyte activation and Ca2+ oscillation-inducing abilities of mouse and human dead (sonicated) spermatozoa." Zygote 17, no. 2 (2009): 175–84. http://dx.doi.org/10.1017/s0967199408005157.

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SummaryIn ICSI procedures, it is well known that the selection of viable (live) spermatozoa and certain types of immobilization prior to injection is very important for obtaining successful results, but unfortunately there are rare situations when only immotile spermatozoa are available (such as in severe asthenozoospermia or necrozoospermia). In such cases, failure of oocyte activation after ICSI often occurs and may be due to the lack of SOAF (sperm-borne oocyte activating factor) activity. In order to investigate the SOAF activities of dead spermatozoa, mouse and human spermatozoa were immo

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Katayama, Mike, Takashi Miyano, Masashi Miyake, and Seishiro Kato. "Progesterone treatment of boar spermatozoa improves male pronuclear formation after intracytoplasmic sperm injection into porcine oocytes." Zygote 10, no. 2 (2002): 95–104. http://dx.doi.org/10.1017/s0967199402002137.

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Boar spermatozoa were prepared for intracytoplasmic sperm injection (ICSI) by two different treatments to facilitate sperm chromatin decondensation and improve fertilisation rates after ICSI in pigs: spermatozoa were either frozen and thawed without cryoprotectants, or treated with progesterone. Morphological changes of the sperm heads after the treatments were examined and then the activation of oocytes and the transformation of the sperm nucleus following ICSI were assessed. After freezing and thawing, the plasma membrane and acrosomal contents over the apical region of sperm head were lost

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Rybouchkin, Andrei V., Paul De Sutter, and Marc Dhont. "Unprotected freezing of human spermatozoa exerts a detrimental effect on their oocyte activating capacity and chromosome integrity." Zygote 4, no. 04 (1996): 263–68. http://dx.doi.org/10.1017/s0967199400003208.

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SummaryThe influence of unprotected freezing of mammalian spermatozoa on their oocyte activating capacity and chromosome integrity is unknown. However, this type of sperm treatment has been used in assisted reproduction by intracytoplasmic sperm injection in cattle and humans. The mouse oocyte injection test was used to analyse the influence of unprotected freezing of human spermatozoa on their reproductive characteristics. Mouse oocytes were microinjected with intact human spermatozoa or spermatozoa treated with two cycles of unprotected freeze-thawing. Oocytes surviving the injection were ei

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Wakayama, T., T. Uehara, Y. Hayashi, and R. Yanagimachi. "The response of mouse oocytes injected with sea urchin spermatozoa." Zygote 5, no. 3 (1997): 229–34. http://dx.doi.org/10.1017/s096719940000366x.

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SummarySea urchin spermatozoa were injected into mature mouse oocytes to determine whether they can activate mouse oocytes and, if so, how they behave within the oocyte cytoplasm of such a distant species. While injection of a single spermatozoon into each oocyte did not activate any of the oocytes, injection of 10 spermatozoa activated about 20%. Within the cytoplasm of unactivated oocytes, sperm heads commonly transformed into chromosome-like structures. When a single spermatozoon was injected, and oocytes were then activated by Sr2+, about 30% of the activated oocytes had both female (mouse

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7

Okitsu, Osamu, Shuji Yamano, and Toshihiro Aono. "Activation of bovine oocytes matured in vitro by injection of bovine and human spermatozoa or their cytosolic fractions." Zygote 9, no. 1 (2001): 89–95. http://dx.doi.org/10.1017/s0967199401001095.

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The aim of this study was to investigate whether bovine spermatozoa possess so-called sperm factor in the cytosolic fraction (CF) which activates bovine oocytes, and whether bovine oocytes matured in vitro are activated by microinjection of CF extracted from spermatozoa of other species. In the first experiment, bovine and human spermatozoa were microinjected into ooplasm of bovine oocytes matured in vitro. Secondly, CF from bovine and human spermatozoa were injected into bovine oocytes. In the third, CF from human spermatozoa was injected into human unfertilised oocytes obtained 18-20 h after

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8

Kobayashi, Toshihiro, Kazue Amemiya, Kana Takeuchi, et al. "Contribution of spermatozoal centrosomes to the microtubule-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)." Zygote 14, no. 1 (2006): 45–51. http://dx.doi.org/10.1017/s0967199406003522.

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Using an interspecies microinsemination assay with bovine oocytes, it was examined whether centrosomes of Antarctic minke whale spermatozoa function as the microtubule-organizing centre (MTOC). Bull and rat spermatozoa were used as positive and negative controls, respectively. Vitrified-warmed bovine mature oocytes were subjected to immunostaining against α-tubulin 4–6 h after intracytoplasmic injection (ICSI) of 5 mM dithiothreitol-treated spermatozoa. Aster formation occurred from whale spermatozoa (33%) and bull spermatozoa (33%), but very little from rat spermatozoa (3%). Activation treatm

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9

Gonzalez-Castro, Raul A., Fabio Amoroso-Sanches, JoAnne E. Stokes, James K. Graham та Elaine M. Carnevale. "Localisation of phospholipase Cζ1 (PLCZ1) and postacrosomal WW-binding protein (WBP2 N-terminal like) on equine spermatozoa and flow cytometry quantification of PLCZ1 and association with cleavage in vitro". Reproduction, Fertility and Development 31, № 12 (2019): 1778. http://dx.doi.org/10.1071/rd19217.

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Oocyte activation is initiated when a fertilising spermatozoon delivers sperm-borne oocyte-activating factor(s) into the oocyte cytoplasm. Candidates for oocyte activation include two proteins, phospholipase Cζ1 (PLCZ1) and postacrosomal WW-binding protein (PAWP; also known as WBP2 N-terminal like (WBP2NL)). We localised PLCZ1 and WBP2NL/PAWP in stallion spermatozoa and investigated the PLCZ1 content and sperm parameters as well as cleavage after intracytoplasmic sperm injection (ICSI). PLCZ1 was identified as 71-kDa protein in the acrosomal and postacrosomal regions, midpiece and principal pi

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Ishchuk, Mariia A., Evgeniia M. Komarova, Elena A. Lesik, et al. "DNA-fragmented sperm ability to bind to the zona pellucida." Journal of obstetrics and women's diseases 72, no. 6 (2023): 63–76. http://dx.doi.org/10.17816/jowd569417.

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BACKGROUND: A high percentage of the infertile couples are classified as having unexplained infertility. At the same time, male partners may have both normozoospermia and high level of sperm DNA fragmentation. The investigation of the selectivity of the oocyte zona pellucida for spermatozoa with fragmented DNA is an extremely promising direction. The novel data could improve sperm selection methods, which in turn will increase the efficiency of IVF cycles. AIM: The aim of this study was to assess the ability of spermatozoa with fragmented DNA to bind to the zona pellucida. MATERIAL

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Smith, Gary D., Clementina Cantatore, and Dana A. Ohl. "Microfluidic Systems for Isolation of Spermatozoa from Testicular Specimens of Non-Obstructive Azoospermic Men: Does/Can It Improve Sperm Yield?" Journal of Clinical Medicine 10, no. 16 (2021): 3667. http://dx.doi.org/10.3390/jcm10163667.

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Intracytoplasmic sperm injection (ICSI) has allowed reproduction options through assisted reproductive technologies (ARTs) for men with no spermatozoa within the ejaculate (azoospermia). In men with non-obstructive azoospermia (NOA), the options for spermatozoa retrieval are testicular sperm extraction (TESE), testicular sperm aspiration (TESA), or micro-surgical sperm extraction (microTESE). At the initial time of spermatozoa removal from the testis, spermatozoa are immobile. Independent of the means of spermatozoa retrieval, the subsequent steps of removing spermatozoa from seminiferous tubu

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Kani, C., M. Takenaka, T. Muneto, M. Yamamoto, and T. Horiuchi. "373 OOCYTE-ACTIVATING CAPACITY OF BOVINE SPERMATOGENIC CELLS AND ACTIVATION PROTOCOLS FOR INTRACYTOPLASMIC INJECTION OF BOVINE ROUND SPERMATIDS." Reproduction, Fertility and Development 19, no. 1 (2007): 302. http://dx.doi.org/10.1071/rdv19n1ab373.

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In vitro spermatogenesis can be applied to generate spermatids or spermatozoa and produce a genetically modified male germ line. Intracytoplasmic injection of the spermatids or spermatozoa is an important technique for effective production of offspring. The objective of this study is to evaluate oocyte-activation capacity of bovine spermatids or spermatozoa and to determine the effective activation treatment for in vitro development of bovine oocytes injected with round spermatids. Cryopreserved testicular spermatogenic cells and cauda epididymal spermatozoa obtained from a 1-year-old Japanese

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13

Li, Chong, Eiji Mizutani, Tetsuo Ono, and Teruhiko Wakayama. "Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI." REPRODUCTION 137, no. 5 (2009): 779–92. http://dx.doi.org/10.1530/rep-08-0476.

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In mammals, ICSI is now a very important tool for both assisted reproductive technology and studying the mechanisms of fertilization. In the latter experiments, it is important to use spermatozoa that have lost their oocyte activation capacity but still retain their developmental potential. In this study, we used high-concentration NaOH to remove oocyte activation potential from spermatozoa, and examined whether normal offspring could be generated from these spermatozoa after ICSI. The spermatozoa were treated with different concentrations of NaOH (1–100 mM) for 1 h and then neutralized with e

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Suarez, S. S. "Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction." Reproduction, Fertility and Development 19, no. 1 (2007): 103. http://dx.doi.org/10.1071/rd06101.

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Artificial insemination with sexed semen, in vitro fertilisation and intracytoplasmic sperm injection have been used to reproduce animals, but often not as successfully as natural mating. Learning more about how spermatozoa normally interact with the female tract can provide inspiration for developing improvements in assisted reproduction. The present review focuses on Bos taurus, because more is known about this species than others. At coitus, bull spermatozoa are deposited into the anterior vagina, where they rapidly enter the cervix. Cervical mucus quickly filters out seminal plasma from sp

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Loren, Jean, and Orly Lacham-Kaplan. "The employment of strontium to activate mouse oocytes: effects on spermatid-injection outcome." Reproduction 131, no. 2 (2006): 259–67. http://dx.doi.org/10.1530/rep.1.00894.

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The present research investigated the effects of various strontium concentrations, in combination with different incubation periods, on mouse parthenogentic oocyte activation and blastocyst development. The results for blastocyst development showed a trend indicating that 10 mM strontium for 3 h was the optimal strontium protocol. Ethanol, an agent that incites oocyte activation via a monotonic rise in calcium, was employed as a control. The outcome of blastocyst formation arising from parthenogenic ethanol activation was significantly less (P < 0.001) than that achieved by the optimal stro

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Cenariu, Mihai, Mihai Borzan, Sorin Dan, Remus Chiorean, and Emoke Pall. "Production of viable bovine embryos by intracytoplasmic sperm injection of oocytes harvested from slaughtered old cows." Cluj Veterinary Journal 26, no. 1 (2021): 7–14. http://dx.doi.org/10.52331/cvj.v26i1.8.

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Abstract: (1) Background: Intracytoplasmic sperm injection (ICSI) is currently used to increase fertilization success by avoiding several oocyte or sperm deficiencies that would normally prevent conception after in vivo fertilization or classical in vitro fertilization. This paper aimed at improving the in vitro fertilization protocol of bovine oocytes, harvested from old cows after slaughtering, using intracytoplasmic sperm injection; (2) Methods: Oocytes were harvested by puncture of follicles from ovaries obtained from slaughtered old cows, followed by aspiration. Out of the 127 cumulus-ooc

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Goto, K., A. Kinoshita, A. Kuroda, Y. Nakanishi, and K. Ogawa. "Cleavage of mouse oocyte after the injection of immobilized killed spermatozoa." Asian-Australasian Journal of Animal Sciences 4, no. 3 (1991): 251–54. http://dx.doi.org/10.5713/ajas.1991.251.

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Tateno, Hiroyuki, Teruhiko Wakayama, W. Steven Ward, and R. Yanagimachi. "Can alcohol retain the reproductive and genetic potential of sperm nuclei? Chromosome analysis of mouse spermatozoa stored in alcohol." Zygote 6, no. 3 (1998): 233–38. http://dx.doi.org/10.1017/s0967199498000173.

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Alcohol is known to preserve genomic DNA and the primary structure of sperm protamines. To determine whether alcohol can retain the genetic and reproductive potential of mammalian sperm nuclei, mature mouse spermatozoa were stored in 70% ethanol or propanol for up to 2 months before injection into oocytes. Live offspring were obtained after injection of spermatozoa stored in 70% ethanol for 1 day at -20 °C. About 20% of the spermatozoa stored under this condition had normal chromosomes. The remaining 80% of spermatozoa and all the spermatozoa stored in 70% ethanol for 2 months had structurally

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Parrella, Alessandra, Llanos Medrano, Jon Aizpurua, and María José Gómez-Torres. "Phospholipase C Zeta in Human Spermatozoa: A Systematic Review on Current Development and Clinical Application." International Journal of Molecular Sciences 25, no. 2 (2024): 1344. http://dx.doi.org/10.3390/ijms25021344.

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During fertilization, the fusion of the spermatozoa with the oocytes causes the release of calcium from the oocyte endoplasmatic reticulum. This, in turn, triggers a series of calcium ion (Ca2+) oscillations, a process known as oocyte activation. The sperm-specific factor responsible for oocyte activation is phospholipase C zeta (PLCζ). Men undergoing intracytoplasmic sperm injection (ICSI) with their spermatozoa lacking PLCζ are incapable of generating Ca2+ oscillation, leading to fertilization failure. The immunofluorescence assay is the most used technique to assess the expression and local

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Watanabe, H., H. Tateno, H. Kusakabe, et al. "Fertilizability and chromosomal integrity of frozen-thawed Bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes." Zygote 15, no. 1 (2007): 9–14. http://dx.doi.org/10.1017/s0967199406003923.

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SUMMARYPrior to attempting the in vitro production of embryos in the Bryde's whale (Balaenoputera edeni), we investigated whether spermatozoa can retain the capacity for oocyte activation and pronucleus formation as well as chromosomal integrity under cryopreservation by using intracytoplasmic sperm injection (ICSI) into mouse oocytes. Regardless of motility and viability, whale spermatozoa efficiently led to the activation of mouse oocytes (90.3–97.4%), and sperm nuclei successfully transformed into male pronucleus within activated ooplasm (87.2–93.6%). Chromosome analysis at the first cleava

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Yazawa, H., K. Yanagida, and A. Sato. "Human round spermatids from azoospermic men exhibit oocyte-activation and Ca2+ oscillation-inducing activities." Zygote 15, no. 4 (2007): 337–46. http://dx.doi.org/10.1017/s0967199407004339.

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SummaryDuring mammalian fertilization, intracellular Ca2+ oscillations are important for both oocyte activation and embryonic development. As the ability of round spermatids (ROS) to induce Ca2+ oscillations and oocyte activation is different between species, we examined Ca2+ oscillation- and oocyte activation-inducing abilities of human ROS originating from patients with non-obstructive azoospermia. Human ROS from 11 non-obstructive azoospermic patients were collected during their TESE–ICSI cycles. Following injection into mature unfertilized mouse oocytes, we examined the oocyte-activating a

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Tanaka, Atsushi, Motoi Nagayoshi, Youichi Takemoto, et al. "Fourteen babies born after round spermatid injection into human oocytes." Proceedings of the National Academy of Sciences 112, no. 47 (2015): 14629–34. http://dx.doi.org/10.1073/pnas.1517466112.

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During the human in vitro fertilization procedure in the assisted reproductive technology, intracytoplasmic sperm injection is routinely used to inject a spermatozoon or a less mature elongating spermatid into the oocyte. In some infertile men, round spermatids (haploid male germ cells that have completed meiosis) are the most mature cells visible during testicular biopsy. The microsurgical injection of a round spermatid into an oocyte as a substitute is commonly referred to as round spermatid injection (ROSI). Currently, human ROSI is considered a very inefficient procedure and of no clinical

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Tang, Van, Huyen Dang Thi, Loc Ly Thai, et al. "#230 : The Effectiveness of Spermatid Injection (ROSI, ELSI) Versus Spermatozoa Injection (ICSI) in Infertility Treatment for Men with Non-Obstructive Azoospermia (NOA)." Fertility & Reproduction 05, no. 04 (2023): 562–63. http://dx.doi.org/10.1142/s2661318223743096.

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Background and Aims: About 1% of men suffer from azoospermia, a condition of having no sperm in the ejaculate. Non-obstructive azoospermia, which affects 60% of azoospermia patients, is the most severe form of male infertility and requires testicular sperm extraction for sperm retrieval. Despite a 50% success rate, if no mature sperm is found, NOA men have no choice become biological fathers. To overcome this, researchers have attempted to use spermatid injection techniques, such as round spermatid injection (ROSI) or elongating/elongated spermatid injection (ELSI), to give hope for pregnancy.

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Edwards, RG. "Cell cycle factors in the human oocyte and the intracytoplasmic injection of spermatozoa." Reproduction, Fertility and Development 7, no. 2 (1995): 143. http://dx.doi.org/10.1071/rd9950143.

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Boitrelle, F., B. Guthauser, L. Alter, et al. "High-magnification selection of spermatozoa prior to oocyte injection: confirmed and potential indications." Reproductive BioMedicine Online 28, no. 1 (2014): 6–13. http://dx.doi.org/10.1016/j.rbmo.2013.09.019.

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Watanabe, Hiroyuki, Junko Akiyama, Mohammed Musharraf Uddin Bhuiyan, and Yutaka Fukui. "Enhanced Oocyte Activation by Intracytoplasmic Injection of Porcine Spermatozoa Pre-treated with Dithiothreitol." Journal of Mammalian Ova Research 26, no. 1 (2009): 54–60. http://dx.doi.org/10.1274/jmor.26.54.

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Rossini, J. B., J. Rodriguez, D. R. Bresnahan, J. E. Stokes, and E. M. Carnevale. "Autogenous transfer of intracytoplasmic sperm injection-produced equine embryos into the uterus of the oocyte donor during the same oestrous cycle." Reproduction, Fertility and Development 31, no. 12 (2019): 1912. http://dx.doi.org/10.1071/rd19253.

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The clinical use of intracytoplasmic sperm injection (ICSI) in horses usually involves the transfer of embryos into recipient mares, resulting in substantial cost increases. This is essential when subfertile mares are oocyte donors; but some donors are fertile, with ICSI compensating for limited or poor-quality spermatozoa. Fertile oocyte donors could carry pregnancies, eliminating the need for a recipient. We assessed the potential of using oocyte donors as recipients for their own ICSI-produced embryos during the same cycle. Donors in oestrus and with large dominant follicles were administer

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Nie, Hua, Yunge Tang, and Weibing Qin. "Beyond Acephalic Spermatozoa: The Complexity of Intracytoplasmic Sperm Injection Outcomes." BioMed Research International 2020 (February 10, 2020): 1–7. http://dx.doi.org/10.1155/2020/6279795.

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This review analyses the genetic mechanisms of acephalic spermatozoa (AS) defects, which are associated with primary infertility in men. Several target genes of headless sperms have been identified but intracytoplasmic sperm injection (ICSI) outcomes are complex. Based on electron microscopic observations, broken points of the sperm neck are AS defects that are based on various genes that can be classified into three subtypes: HOOK1, SUN5, and PMFBP1 genes of subtype II; TSGA10 and BRDT genes of subgroup III, while the genetic mechanism(s) and aetiology of AS defects of subtype I have not been

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Trávník, Pavel, Michal Ješeta, Renata Hüttelová, et al. "Assisted oocyte activation." Česká gynekologie 88, no. 6 (2023): 459–62. http://dx.doi.org/10.48095/cccg2023459.

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Objective: Currently, there is a rapid increase in studies on assisted oocyte activation, which can significantly improve the process of in vitro fertilization. Fertilization of oocytes by conventional methods and by intracytoplasmic sperm injection can be affected by insufficient activation of the oocyte. The reason is mainly deviations in the enzymatic equipment of sperm or oocytes or a non-functional activation cascade. In many cases, fertilization can be achieved using artificial oocyte activation by applying calcium ion donors to the oocytes after sperm microinjection. However, opinions o

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Wang, Bin, H. Baldassarre, J. Pierson, F. Cote, K. M. Rao, and C. N. Karatzas. "The in vitro and in vivo development of goat embryos produced by intracytoplasmic sperm injection using tail-cut spermatozoa." Zygote 11, no. 3 (2003): 219–27. http://dx.doi.org/10.1017/s0967199403002260.

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The objective of this study was to assess the efficacy of a novel intracytoplasmic sperm injection (ICSI) procedure, as well as the in vitro and in vivo developmental competence of goat embryos produced by ICSI. Oocyte-cumulus complexes recovered by LOPU from donors stimulated with gonadotrophins were matured in vitro. Fresh goat semen was used for ICSI following Percoll gradient washing. Tail-cut spermatozoa were microinjected into the ooplasm of goat oocytes using a piezo micropipette-driving system (PiezoDrill). In order to assess developmental competence, the ICSI-derived zygotes were cult

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Van Steirteghem, AC, P. Nagy, J. Liu, et al. "Intracytoplasmic sperm injection — ICSI." Reproductive Medicine Review 3, no. 3 (1994): 199–207. http://dx.doi.org/10.1017/s0962279900000879.

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For more than a decade in vitro fertilization (IVF) has been successful in the treatment of couples with long-standing infertility due to various aetiologies such as tubal disease, male-factor infertility, unexplained infertility and endometriosis. The usual fertilization rate in IVF for nonmale infertility cases is 60–70% of the inseminated cumulus-oocyte complexes and in andrological infertility it is only 20–30%. The lower the number of normally fertilized oocytes, the less chance there is of available embryos, so that patients may have no embryos to transfer. It has been the experience of

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Kim, Bong-Ki, Sun Hong Cheon, Youn Jeong Lee, Sun Ho Choi, Xiang Shun Cui, and Nam-Hyung Kim. "Pronucleus formation, DNA synthesis and metaphase entry in porcine oocytes following intracytoplasmic injection of murine spermatozoa." Zygote 11, no. 3 (2003): 261–70. http://dx.doi.org/10.1017/s0967199403002314.

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The onset of pronucleus formation and DNA synthesis in porcine oocytes following the injection of porcine or murine sperm was determined in order to obtain insights into species-specific paternal factors that contribute to fertilisation. Similar frequencies of oocytes with female pronuclei were observed after injection with porcine sperm or with murine sperm. In contrast, male pronuclei formed 8-9 h following the injection of porcine sperm, and 6-8 h following the injection of murine sperm. After pronucleus formation maternally derived microtubules were assembled and appeared to move both male

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Wei, Hong, and Yukata Fukui. "Births of calves derived from embryos produced by intracytoplasmic sperm injection without exogenous oocyte activation." Zygote 10, no. 2 (2002): 149–53. http://dx.doi.org/10.1017/s0967199402002204.

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Tail-cut bovine spermatozoa were microinjected into ooplasmic lipid polarised, in vitro matured bovine oocytes using a piezomicropipette-driving system. No exogenous oocyte activation treatment was used. Of the sperm-injected oocytes, 86.3% were activated, 71.8% cleaved and 22.7% developed to the blastocyst stage. The average cell count of the blastocysts was 122.5 ± 15 and a majority (81.8%) of the blastocysts were cytologically normal (diploid). When transferred to recipient cows, 5 of 8 blastocysts developed to fetuses and 4 of 7 recipients became pregnant. Normal offspring were born.

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Basnet, Robin Bahadur, Mira Thapa, Rashmi Shrish, and Preeti Bista. "Testicular Sperm Extraction Techniques in Subfertile Males." Nepalese Medical Journal 3, no. 1 (2020): 276–78. http://dx.doi.org/10.3126/nmj.v3i1.28296.

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Introduction: Assisted conception is an option for subfertile couples. Surgical sperm retrieval by testicular sperm aspiration and testicular sperm extraction are widely used safe techniques to yield sperm for intracytoplasmic sperm injection. Experience with these techniques is presented. Materials and Methods: A retrospective study of testicular sperm retrieval for assisted reproduction is presented. Testicular sperm aspiration is attempted on all azoospermic males with normal sexual characteristics. Testicular sperm extraction is attempted on consenting patients where aspiration has f

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Asada, Masatsugu, Hong Wei, Rie Nagayama, et al. "An attempt at intracytoplasmic sperm injection of frozen-thawed minke whale (Balaenoptera bonaerensis) oocytes." Zygote 9, no. 4 (2001): 299–307. http://dx.doi.org/10.1017/s0967199401001344.

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Little is known about the characteristics of fertilisation events in minke whales. Cryopreserved minke whale oocytes and spermatozoa do not fertilise in a standard IVF. This study was conducted to investigate the pronucleus formation ability of cryopreserved minke whale oocytes and their subsequent development following intracytoplasmic sperm injection (ICSI). In experiment 1, frozen-thawed minke whale immature oocytes were cultured for in vitro maturation (IVM) in a maturation medium (TCM199) supplemented with either porcine follicle stimulating hormone (pFSH)/estradiol-17β(E2) or pregnant ma

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Zhang, Liuguang, Yuhu Li, Yuqun Huang, and Zongqiang Li. "Successful birth after ICSI with testicular immotile spermatozoa from a patient with total MMAF in the ejaculates: a case report." Zygote 30, no. 2 (2021): 169–75. http://dx.doi.org/10.1017/s096719942100068x.

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SummaryThere has been no report on the outcome of vitrified blastocyst transfer from a vitrified oocyte injected with immotile testicular spermatozoa with only multiple morphological abnormalities of the sperm flagella (MMAF). A couple diagnosed with MMAF returned to the clinic to attempt pregnancy using their vitrified oocytes. Testicular spermatozoa were injected intracytoplasmically, and the following intracytoplasmic sperm injection results were observed. In the second cycle, surplus vitrified oocytes and testicular retrieved sperm were used, but no pregnancy ensued. In the third cycle, a

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Merlo, B., P. Gugole, E. Iacono, et al. "142 Horse oocyte intracytoplasmic sperm injection with freeze-dried and frozen donkey spermatozoa: preliminary results." Reproduction, Fertility and Development 35, no. 2 (2022): 199. http://dx.doi.org/10.1071/rdv35n2ab142.

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Takahashi, C., S. Mizuta, R. Nishiyama, et al. "Effect of artificial oocyte activation in intracytoplasmic sperm injection using testicular spermatozoa on sibling oocytes." Fertility and Sterility 104, no. 3 (2015): e317. http://dx.doi.org/10.1016/j.fertnstert.2015.07.993.

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Prasetyo, Dony, Asda Laining, and Agus Oman Sudrajat. "Reproductive performances of wild male tiger shrimp Penaeus monodon post-injection of oocyte developer without eyestalk ablation." Jurnal Akuakultur Indonesia 16, no. 2 (2017): 193. http://dx.doi.org/10.19027/jai.16.2.193-204.

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<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><em> </em></p><p class="NoParagraphStyle">The aim of this study was to evaluate the effect of oodev on the gonadal maturation and characteristic of spermatophore and spermatozoa produced by the wild male black tiger shrimp against eyestalk ablation. The treatments were two doses of oodev injection at 0.5 (OD0.5) and 1 (OD1.0) mL/kg of body weight and a control was eyestalk ablation (AB). The male stock of tiger shrimp used was from wild with body weight ran

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Prasetyo, Dony, Asda Laining, and Agus Oman Sudrajat. "Reproductive performances of wild male tiger shrimp, Penaeus monodon post-injection of oocyte developer without eyestalk ablation." Jurnal Akuakultur Indonesia 16, no. 2 (2017): 204. http://dx.doi.org/10.19027/jai.16.2.204-215.

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<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><em> </em></p><p class="NoParagraphStyle">The aim of this study was to evaluate the effect of oodev on the gonadal maturation and characteristic of spermatophore and spermatozoa produced by the wild male black tiger shrimp against eyestalk ablation. The treatments were two doses of oodev injection at 0.5 (OD0.5) and 1 (OD1.0) mL/kg of body weight and a control was eyestalk ablation (AB). The male stock of tiger shrimp used was from wild with body weight ran

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Yanagimachi, R. "Gamete manipulation for development: new methods for conception." Reproduction, Fertility and Development 13, no. 1 (2001): 3. http://dx.doi.org/10.1071/rd00047.

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Mammalian oocytes microsurgically injected with spermatozoa can develop into normal offspring. Apparently the oocyte has the ability to decompose or eliminate such sperm components as the plasma membrane and acrosomal contents, which normally do not enter its cytoplasm. Species in which normal offspring were obtained by direct sperm injection include: human, mouse, rabbit, horse, sheep, cattle, pig, and monkey. In the mouse, normal offspring can also be obtained routinely by the injection of round spermatid nuclei into oocytes. This suggests that all post-meiotic modifications of spermatozoa (

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Yong, Hwan Yul, Ji Young Hong, Sun Il Pak, et al. "Effect of centrifugation and electrical activation on male pronucleus formation and embryonic development of porcine oocytes reconstructed with intracytoplasmic sperm injection." Reproduction, Fertility and Development 17, no. 5 (2005): 557. http://dx.doi.org/10.1071/rd04022.

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Oocyte centrifugation and electrical activation are commonly used in intracytoplasmic sperm injection (ICSI) of bovine and porcine oocytes, to facilitate visual identification of sperm release into the ooplasm and to support oocyte activation following injection with tail membrane-damaged sperm. The present study evaluated the necessity of these steps in porcine modified ICSI. In the first series of experiments, in vitro-matured gilt oocytes with or without centrifugation were injected with head membrane-damaged spermatozoa aspirated tail first. Oocytes without centrifugation exhibited a signi

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Ogonuki, N., K. Inoue, H. Miki, et al. "322 DIFFERENTIAL DEVELOPMENT OF RABBIT EMBRYOS FOLLOWING MICROINSEMINATION USING SPERM AND SPERMATIDS." Reproduction, Fertility and Development 17, no. 2 (2005): 312. http://dx.doi.org/10.1071/rdv17n2ab322.

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Microinsemination is a technique that delivers male germ cells directly into the ooplasm. The efficiency of fertilization and subsequent embryo development after microinsemination varies with species and the male germ cells used. This study examined the developmental ability of rabbit embryos in vitro and in vivo following microinsemination using haploid male germ cells at different stages. First, we injected rabbit spermatozoa, elongated spermatids, and round spermatids into mouse oocytes to assess their oocyte-activating capacity. Mouse oocytes are a good experimental model for assessing the

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Wirtu, G., C. E. Pope, M. C. Gomez, R. A. MacLean, D. L. Paccamonti, and B. L. Dresser. "274 LYSOLECITHIN TREATMENT OF ELAND, BONGO, AND BOVINE SPERMATOZOA AND CLEAVAGE OF BOVINE OOCYTES AFTER INTERSPECIES INTRACYTOPLASMIC SPERM INJECTION." Reproduction, Fertility and Development 20, no. 1 (2008): 217. http://dx.doi.org/10.1071/rdv20n1ab274.

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Compared to success rates in human, intracytoplasmic sperm injection (ICSI) is inefficient in ungulate species. Although factors such as injection of membrane-intact sperm and toxic effects of acrosome contents are suspected causes, the reasons for the inefficiency are unclear. A recent report in mice demonstrated that ICSI using spermatozoa treated with a physiological detergent, lysolecithin, improved oocyte activation, cleavage, and offspring production after embryo transfer (Morozumi K et al. 2006 PNAS 109, 17 661–17 666). The objectives of the present study were to evaluate the effects of

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Tesarik, Jan, and Raquel Mendoza Tesarik. "Sperm-Derived Dysfunction of Human Embryos: Molecular Mechanisms and Clinical Resolution." International Journal of Molecular Sciences 26, no. 13 (2025): 6217. https://doi.org/10.3390/ijms26136217.

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In addition to the male genome, the fertilizing spermatozoon delivers to the oocyte several factors whose deficiency can cause embryo dysfunction. Sperm oocyte-activating factor, identified as phoshoplipase C zeta (PLCζ), drives oocyte exit from meiotic arrest through a signaling pathway initiated by periodic rises of free cytosolic Ca2+ concentration (calcium oscillations). Sperm centrioles, together with oocyte proteins, form centrosomes that are responsible for aster formation, pronuclear migration, and DNA polarization before nuclear syngamy and subsequent mitotic divisions. Sperm DNA frag

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Li, Qiuyan, Jian Hou, Sheng Wang, et al. "Viable rabbits derived from oocytes by intracytoplasmic injection of spermatozoa from an infertile male." Zygote 17, no. 2 (2009): 157–62. http://dx.doi.org/10.1017/s0967199408005078.

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SummaryIntracytoplasmic sperm injection (ICSI) is an assisted fertilization technique and has been widely applied in human medicine to overcome some obstacles of infertility. However, this technology has not yet been used as a mainstream technique for animal production, including the rabbit, due to its limited success. The aim of this study was to improve ICSI techniques and establish an efficient ICSI method for rabbits. Spermatozoa used for ICSI were collected from mature New Zealand white male rabbits. They were washed two to three times with HEPES-buffered TCM199 containing 10% FBS and the

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Nakai, M., N. Kashiwazaki, N. Maedomari, et al. "376 EFFECT OF SPERM TREATMENT ON THE ABILITY TO ACTIVATE OOCYTES AFTER ICSI IN PIGS." Reproduction, Fertility and Development 19, no. 1 (2007): 303. http://dx.doi.org/10.1071/rdv19n1ab376.

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During fertilization, sperm penetration (gamete membrane fusion and exposure of sperm cytoplasm) allows oocyte activation (resumption of oocyte meiosis, pronuclear formation, etc.) by inducing an elevation of the intracellular free Ca2+ concentration. So a spermatozoon ought to be able to fully activate an oocyte. However, in pig ICSI oocytes, although a spermatozoon is injected successfully into ooplasm, complete activation is deficient in some of the oocytes. A variety of sperm pre-treatments before ICSI have been reported; however, there is a possibility that the treatment affects the abili

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Jiang, Man-Xi, Yue-Liang Zheng, Shu-Zhen Liu, et al. "Effects of oocyte collection time and injection position on pronucleus formation and blastocyst development in round spermatid injection in mouse." Zygote 13, no. 3 (2005): 249–53. http://dx.doi.org/10.1017/s0967199405003308.

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The injection of spermatozoa into mouse, human and rabbit oocytes at specific times and positions can result in different rates of viable embryo development. However, it is not clear how the timing and position of round spermatid injection (ROSI) affect pronucleus (PN) formation and blastocyst development of mice. First, we determined the changes in relative position of the first polar body and the spindle, carried out ROSI from 11.5 to 13 h post-hCG administration, then activated by Sr2+, and finally compared the development of ROSI zygotes, including the formation of pronuclei and developmen

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Borges, E., D. P. A. F. Braga, T. C. S. Bonetti, A. Iaconelli, and J. Franco. "Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa." Fertility and Sterility 90 (September 2008): S190. http://dx.doi.org/10.1016/j.fertnstert.2008.07.673.

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Borges Jr., Edson, Daniela Paes de Almeida Ferreira Braga, Tatiana Carvalho de Sousa Bonetti, Assumpto Iaconelli Jr., and José Gonçalves Franco Jr. "Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa." Fertility and Sterility 92, no. 1 (2009): 131–36. http://dx.doi.org/10.1016/j.fertnstert.2008.04.046.

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