Relevant bibliographies by topics / ITS 16S-23S

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  2. Dissertations / Theses
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Academic literature on the topic 'ITS 16S-23S'

Author: Grafiati
Published: 4 June 2025
Last updated: 1 August 2025

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Journal articles on the topic "ITS 16S-23S"

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Song, Jaekyeong, Seong-Chan Lee, Jun-Won Kang, Hyung-Jin Baek, and Joo-Won Suh. "Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S–23S rDNA internally transcribed spacer sequences." International Journal of Systematic and Evolutionary Microbiology 54, no. 1 (2004): 203–9. http://dx.doi.org/10.1099/ijs.0.02624-0.

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The 16S rRNA gene sequences for 34 strains, including 11 isolates, were determined to classify scab-causing Streptomyces spp. and relatives isolated from potato scab lesions collected in Jeju, Korea. The 16S–23S rDNA internally transcribed spacer (ITS) sequences were determined to investigate whether the 16S–23S ITS region is useful for analysing intra- and interspecific relationships in these bacteria. On the basis of phylogenetic analysis of 16S rRNA gene sequences, most of the isolates were classified as Streptomyces scabiei and Streptomyces acidiscabies. Isolate KJO61 was placed in an ambi
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Cuscó, Anna, Carlotta Catozzi, Joaquim Viñes, Armand Sanchez, and Olga Francino. "Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon." F1000Research 7 (August 1, 2019): 1755. http://dx.doi.org/10.12688/f1000research.16817.2.

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Background: Profiling the microbiome of low-biomass samples is challenging for metagenomics since these samples are prone to contain DNA from other sources (e.g. host or environment). The usual approach is sequencing short regions of the 16S rRNA gene, which fails to assign taxonomy to genus and species level. To achieve an increased taxonomic resolution, we aim to develop long-amplicon PCR-based approaches using Nanopore sequencing. We assessed two different genetic markers: the full-length 16S rRNA (~1,500 bp) and the 16S-ITS-23S region from the rrn operon (4,300 bp). Methods: We sequenced a
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Man, Si Ming, Nadeem O. Kaakoush, Sophie Octavia, and Hazel Mitchell. "The Internal Transcribed Spacer Region, a New Tool for Use in Species Differentiation and Delineation of Systematic Relationships within the Campylobacter Genus." Applied and Environmental Microbiology 76, no. 10 (2010): 3071–81. http://dx.doi.org/10.1128/aem.02551-09.

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ABSTRACT The Campylobacter genus consists of a number of important human and animal pathogens. Although the 16S rRNA gene has been used extensively for detection and identification of Campylobacter species, there is currently limited information on the 23S rRNA gene and the internal transcribed spacer (ITS) region that lies between the 16S and 23S rRNA genes. We examined the potential of the 23S rRNA gene and the ITS region to be used in species differentiation and delineation of systematic relationships for 30 taxa within the Campylobacter genus. The ITS region produced the highest mean pairw
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Huang, Y., G. W. Stemke, and J. A. Robertson. "An unusual rRNA gene organization in Mycoplasma fermentans (incognitus strain)." Canadian Journal of Microbiology 41, no. 4-5 (1995): 424–27. http://dx.doi.org/10.1139/m95-056.

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The macro-restriction map of Mycoplasma fermentans (incognitus strain) was constructed and its rRNA genes were located on the map. It was found that this organism contains two sets of rRNA genes. The 16S and 23S rRNA genes were closely linked as two clusters. However, both 5S rRNA genes were separated from the 16S and 23S genes. The two 16S–23S rRNA gene clusters were arranged in an unusual tail to tail orientation.Key words: physical map, rRNA, Mycoplasma fermentans, genome, gene organization.
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MIKHAILYUK, TATIANA, OKSANA VINOGRADOVA, ANDREAS HOLZINGER, KARIN GLASER, YURI AKIMOV, and ULF KARSTEN. "Timaviella dunensis sp. nov. from sand dunes of the Baltic Sea, Germany, and emendation of Timaviella edaphica (Elenkin) O.M. Vynogr. & Mikhailyuk (Synechococcales, Cyanobacteria) based on an integrative approach." Phytotaxa 532, no. 3 (2022): 192–208. http://dx.doi.org/10.11646/phytotaxa.532.3.1.

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Timaviella Sciuto & Moro is a recently established cryptic genus of cyanobacteria separated from the morphologically close Leptolyngbya due to clear differences in the 16S rRNA gene sequence and the 16S-23S ITS region secondary structure. Conducting research on biological soil crusts in coastal ecotopes of Ukraine and Germany, we repeatedly observed thin filamentous cyanobacteria morphologically corresponding to the common terrestrial species Leptolyngbya edaphica (Elenkin) Anagnostidis & Komárek. Molecular data based on 16S rRNA gene sequence comparison of the original strains of the
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Wolde-meskel, Endalkachew, Zewdu Terefework, Åsa Frostegård, and Kristina Lindström. "Genetic diversity and phylogeny of rhizobia isolated from agroforestry legume species in southern Ethiopia." International Journal of Systematic and Evolutionary Microbiology 55, no. 4 (2005): 1439–52. http://dx.doi.org/10.1099/ijs.0.63534-0.

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The genetic diversity within 195 rhizobial strains isolated from root nodules of 18 agroforestry species (15 woody and three herbaceous legumes) growing in diverse ecoclimatic zones in southern Ethiopia was investigated by using PCR–RFLP of the ribosomal operon [16S rRNA gene, 23S rRNA gene and the internal transcribed spacer (ITS) region between the 16S rRNA and 23S rRNA genes] and 16S rRNA gene partial sequence (800 and 1350 bp) analyses. All of the isolates and the 28 reference strains could be differentiated by using these methods. The size of the ITS varied among test strains (500–1300 bp
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Gaysina, Lira A., Jeffrey R. Johansen, Aniket Saraf, Rezeda Z. Allaguvatova, Sagarika Pal, and Prashant Singh. "Roholtiella volcanica sp. nov., a New Species of Cyanobacteria from Kamchatkan Volcanic Soils." Diversity 14, no. 8 (2022): 620. http://dx.doi.org/10.3390/d14080620.

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During a study of biodiversity of cyanobacteria in Gorely volcano soils (Kamchatka Peninsula), a strain of heterocytous, a false branching cyanobacterium with gradually tapered filaments, was isolated. Prominent features of the strain were purplish-grey trichomes and firm, distinct multilayered sheaths. Based on the results obtained from the morphological, ecological, and phylogenetic analysis using the 16S rRNA and 16S–23S ITS region, 16S–23S ITS secondary structure analysis, comparison of flanking regions of BoxB and V3 helices, and the p-distance between the 16S–23S ITS region, we describe
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Molinares-Pacheco, Cristian, Julieta Mariana Muñoz-Morales, Adriana Carbajal-Armenta, Ana Laura Hernández-Tenorio, Alejandro Cueto-Becerra, and Jesús Muñoz-Rojas. "Métodos de identificación molecular para Gluconacetobacter diazotrophicus." Alianzas y Tendencias BUAP 6, no. 21 (2021): 28–44. https://doi.org/10.5281/zenodo.5104709.

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<strong>RESUMEN</strong> Los m&eacute;todos para la identificaci&oacute;n de microorganismos se valen de sus caracter&iacute;sticas morfol&oacute;gicas, fisiol&oacute;gicas, prote&oacute;micas y gen&oacute;micas. En la identificaci&oacute;n tradicional se emplean t&eacute;cnicas dependientes de cultivo que pueden presentar inconvenientes seg&uacute;n el tipo de microorganismo a identificar, su capacidad de crecimiento, su parecido con alg&uacute;n otro microbio y el tiempo total para su identificaci&oacute;n. El uso de PGPB en la agricultura ha repercutido positivamente reduciendo costos de pr
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Morandi, Stefano, Paola Cremonesi, Milena Povolo, and Milena Brasca. "Enterococcus lactis sp. nov., from Italian raw milk cheeses." International Journal of Systematic and Evolutionary Microbiology 62, Pt_8 (2012): 1992–96. http://dx.doi.org/10.1099/ijs.0.030825-0.

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Ten atypical Enterococcus strains were isolated from Italian raw milk cheeses. The 16S rRNA gene, phenylalanyl-tRNA synthase alpha subunit (pheS), RNA polymerase alpha subunit (rpoA) and the 16S–23S rRNA intergenic transcribed spacer (ITS) sequences, randomly amplified polymorphic DNA (RAPD) PCR and the phenotypic properties revealed that the isolates represent a novel enterococcal species. On the basis of 16S rRNA gene sequence analysis, the isolates were closely related to Enterococcus hirae ATCC 8043T, Enterococcus durans CECT 411T and Enterococcus faecium ATCC 19434T, with 98.8, 98.9 and 9
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Asil, A., No author No author, S. Sanousi, et al. "Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan." Open Veterinary Journal 5, no. 2 (2014): 16. http://dx.doi.org/10.5455/ovj.2014.v4.i1.p16.

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This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments ou
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Dissertations / Theses on the topic "ITS 16S-23S"

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Santos, Viviane Piccin dos [UNESP]. "Filogenia molecular de cianobactérias baseada em sequências do 16S-23S-ITS rDNA e PC-IGS: investigação de transferência lateral do PC." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/87867.

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Made available in DSpace on 2014-06-11T19:23:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-17Bitstream added on 2014-06-13T19:28:52Z : No. of bitstreams: 1 santos_vp_me_rcla.pdf: 692384 bytes, checksum: 87577700198a680d7662c5fe0990efe7 (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>As cianobactérias apresentam uma ampla variabilidade fenotípica e ecológica. Porém, esta variabilidade, muitas vezes, não corresponde à sua diversidade genética. Assim, o uso de marcadores moleculares é fundamental para os estudos de filogenia neste grupo. Entretanto, a fi
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Santos, Viviane Piccin dos. "Filogenia molecular de cianobactérias baseada em sequências do 16S-23S-ITS rDNA e PC-IGS : investigação de transferência lateral do PC /." Rio Claro : [s.n.], 2011. http://hdl.handle.net/11449/87867.

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Orientador: Maria do Carmo Bittencourt de Oliveira<br>Banca: Luiz Henrique Zanini Branco<br>Banca: Mariana Cabral de Oliveira<br>Resumo: As cianobactérias apresentam uma ampla variabilidade fenotípica e ecológica. Porém, esta variabilidade, muitas vezes, não corresponde à sua diversidade genética. Assim, o uso de marcadores moleculares é fundamental para os estudos de filogenia neste grupo. Entretanto, a filogenia molecular enfrenta um desafio na seleção dos marcadores devido à ocorrência relativamente frequente da transferência de genes de forma lateral entre os procariotos. Em cianobactérias
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Mesfin, Melaku. "COMPARATIVE STUDY OF CYANOBACTERIA OF DESERT AND SEMI-DESERT CRUSTS OF TWO DIFFERENT CONTINENTS: AFRICA (ETHIOPIA) AND NORTH AMERICA (USA)." Cleveland State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1245854204.

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Buch, Bruna. "Caracterização taxonômica e prospecção de toxinas de cianobactérias bentônicas de ambientes lênticos da região noroeste do estado de São Paulo." Universidade Estadual Paulista (UNESP), 2018. http://hdl.handle.net/11449/180623.

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Submitted by Bruna Buch (bruna.buch@gmail.com) on 2019-01-31T14:18:48Z No. of bitstreams: 1 BrunaBuch_Tese.pdf: 6059154 bytes, checksum: ab596bb37969e3a6ac3aea0557b5363e (MD5)<br>Rejected by Elza Mitiko Sato null (elzasato@ibilce.unesp.br), reason: Solicitamos que realize correções na submissão seguindo as orientações abaixo: Problema 01) Solicitamos que corrija o ano descrita na capa para 2019, o ano de entrega da dissertação na Seção Técnica de Pós-Graduação Problema 02) Segundo a Portaria nº 206, de 4 de setembro de 2018, todos os trabalhos que tiveram financiamento CAPES deve c
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Villanueva, Chelsea Denise. "Molecular and Evolutionary Analysis of Cyanobacterial Taxonomic Methods." UNF Digital Commons, 2018. https://digitalcommons.unf.edu/etd/810.

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Cyanobacteria are a group of photo-oxygenic bacteria found in nearly every ecosystem, but much cyanobacterial diversity, in various habitats, has yet to be explored. Cyanobacteria are often conspicuous components of photosynthetic flora, providing significant carbon and nitrogen inputs to surrounding systems. As possible primary colonizers of stone substrates not native to this region, cyanobacteria isolated from headstones may provide biogeographically informative data. An exploratory study of lichen-dominated microbial consortia, growing on headstones, was conducted to isolate and identify n
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BOHUNICKÁ, Markéta. "Towards a modern revision of the cyanobacteria, a critically important prokaryotic phylum." Doctoral thesis, 2015. http://www.nusl.cz/ntk/nusl-200683.

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With an adoption of modern methods of polyphasic approach to the study of cyanobacteria, an increased demand for the revision of the traditional taxonomy has emerged. This thesis is devoted to the systematic revisions of selected terrestrial cyanobacteria at several taxonomic levels. The methodology included thorough morphological characterization of cultured cyanobacterial strains using light and electron microscopy complemented with analyses of the molecular data: DNA sequencing, phylogenetic analyses based on 16S rRNA gene and the adjacent 16S-23S ITS region, and comparison of the predicted
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Wei, Yu-Fang, and 魏郁芳. "Construction of a Sequence Database of 16S-23S rDNA Spacer (ITS) of Nonfermentative Gram-Negative Bacilli and Identification of Acinetobacter calcoaceticus-Acinetobacter baumannii (Acb) Complex by ITS Sequence Analysis." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/61532068069944098440.

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碩士<br>國立成功大學<br>醫學工程研究所<br>91<br>Members of the genus Acinetobacter and other nonfermentative gram-negative bacteria are clinically important pathogens. However, clinical laboratories still lack simple methods that allow accurate identification of these species. For example, species of the Acinetobacter calcoaceticus— Acinetobacter baumannii complex (Acb complex) that includes Acinetobacter calcoaceticus (genospecies 1), A. baumannii (genospecies 2), unnamed genospecies 3 and 13TU are difficult to be differentiated. In fact, a complete discrimination between these species is possible only by D
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Chia-Wen, Cheng, and 鄭嘉雯. "Using of conjugation method, enterotoxin gene detection and 16S-23S rDNA ITS sequence analysis to study the phylogenetic relationship between B. cereus and B. thuringiensis." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/21559640910137020802.

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碩士<br>國立中興大學<br>食品科學系<br>89<br>In this research, plasmid pBTIAb was the marker in mating experiment, while strain BT16Te was the donor. The results showed that plasmid was conjugally transferable between B. cereus and B. thuringiensis. B. cereus is food-poisoning for it''s production of hemolysin BL(HBL) and non-hemolytic enterotoxin (NHE). This research adopted primer sets hblA1/hblA2, nheB1500S/nheB2269A, and nheC2820S/nheC3401A, as well as, from this Lab, sets of L2F/L2b, L1b/L1F and nheaF/nheaR. Via polymerase chain reaction (PCR), 79 strains of B. thuringiensis from diffe
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Book chapters on the topic "ITS 16S-23S"

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Gürtler, Volker, Gangavarapu Subrahmanyam, Malathi Shekar, Biswajit Maiti, and Indrani Karunasagar. "Bacterial Typing and Identification By Genomic Analysis of 16S–23S rRNA Intergenic Transcribed Spacer (ITS) Sequences." In Methods in Microbiology. Elsevier, 2014. http://dx.doi.org/10.1016/bs.mim.2014.07.004.

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Galperin, Michael Y., and Eugene V. Koonin. "Complete Prokaryotic Genomes: Reading and Comprehension." In Systems Biology. Oxford University PressNew York, NY, 2006. http://dx.doi.org/10.1093/oso/9780195300819.003.0006.

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Abstract The windfall of complete genomic sequences in the past ten years has dramatically changed the face of biology, which has started to lose its purely descriptive character. Instead, biology is gradually becoming a quantitative discipline that deals with firmly established numerical values and can be realistically described by mathematical models. Indeed, we now know that, for example, the bacteriumMycoplasma genitalium has a single chromosome which consists of 580,074 base pairs and carries genes for three ribosomal RNAs (5S, 16S, and 23S), 36 tRNAs, and 478 proteins [1,2]. We also know
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